journal
MENU ▼
Read by QxMD icon Read
search

Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology

journal
https://www.readbyqxmd.com/read/28196764/nuclear-and-membrane-progestin-receptors-in-the-european-eel-characterization-and-expression-in-vivo-through-spermatogenesis
#1
Marina Morini, David S Peñaranda, María C Vílchez, Rasoul Nourizadeh-Lillabadi, Anne-Gaëlle Lafont, Sylvie Dufour, Juan F Asturiano, Finn-Arne Weltzien, Luz Pérez
Characterization of all the progestin receptor genes (PRs) found in the European eel has been performed. There were five membrane PRs (mPRs): mPRα (alpha), mPRAL1 (alpha-like1), mPRAL2 (alpha-like2), mPRγ (gamma), mPRδ (delta) and two nuclear PRs (nPRs or PGRs): pgr1 and pgr2. In silico studies showed that the C and E(F) domains of Pgr are well conserved among vertebrates whereas the A/B domain is not. Phylogeny and synteny analyses suggest that eel duplicated pgr (pgr1 and pgr2) originated from the teleost-specific third whole genome duplication (3R)...
February 11, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28192243/the-influence-of-mechanical-ventilation-on-physiological-parameters-in-ball-pythons-python-regius
#2
Sashia L Jakobsen, Catherine J A Williams, Tobias Wang, Mads F Bertelsen
Mechanical ventilation is widely recommended for reptiles during anesthesia, and while it is well-known that their low ectothermic metabolism requires much lower ventilation than in mammals, very little is known about the influence of ventilation protocol on the recovery from anesthesia. Here, 15 ball pythons (Python regius) were induced and maintained with isoflurane for 60min at one of three ventilation protocols (30, 125, or 250ml min(-1)kg(-1) body mass) while an arterial catheter was inserted, and ventilation was then continued on 100% oxygen at the specified rate until voluntary extubation...
February 10, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28192242/molecular-cloning-characterization-and-temporal-expression-of-the-clock-genes-period-and-timeless-in-the-oriental-river-prawn-macrobrachium-nipponense-during-female-reproductive-development
#3
SuHua Chen, Hui Qiao, HongTuo Fu, Shengming Sun, WenYi Zhang, ShuBo Jin, Yongsheng Gong, Sufei Jiang, Weiyi Xiong, YanWu
The circadian clock is crucial for sustaining rhythmic biochemical, physiological, and behavioral processes in living creatures. In this study, we isolated and characterized two circadian clock genes in Macrobrachium nipponense, period (Mnper) and timeless (Mntim). The complete Mnper cDNA measures 4283bp in length with an open reading frame encoding 1292 amino acids, including functional domains such as PER-ARNT-SIM (PAS), cytoplasmic localization domain (CLD), TIM interaction site (TIS), and nuclear localization signal (NLS)...
February 10, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28192241/molecular-cloning-mrna-expression-and-characterization-of-membrane-bound-hemoglobin-in-oriental-river-prawn-macrobrachium-nipponense
#4
Shengming Sun, Fujun Xuan, Hongtuo Fu, Jian Zhu, Xianping Ge, Xugan Wu
Most hemoglobins are respiratory proteins and are ubiquitous in animals, bacteria, fungi, protists, and plants. In this study, we describe a membrane-bound hemoglobin in the oriental river prawn Macrobrachium nipponense (MnHb), which also expresses hemocyanin. MnHb cDNA was cloned using the rapid amplification of cDNA ends (RACE) approach, which afforded a 1201bp gene encoding a 193 amino acid polypeptide. Bioinformatic evaluation suggested MnHb is membrane anchored by N-myristoylation, and immunofluorescence confirmed its location in the membrane of chief cells in the gill...
February 10, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28188883/effects-of-pinealectomy-on-the-neuroendocrine-reproductive-system-and-locomotor-activity-in-male-european-sea-bass-dicentrarchus-labrax
#5
Mairi Cowan, José A Paullada-Salmerón, José Fernando López-Olmeda, Francisco Javier Sánchez-Vázquez, José A Muñoz-Cueto
The seasonally changing photoperiod controls the timing of reproduction in most fish species, however, the transduction of this photoperiodic information to the reproductive axis is still unclear. This study explored the potential role of two candidate neuropeptide systems, gonadotropin-inhibitory hormone (Gnih) and kisspeptin, as mediators between the pineal organ (a principle transducer of photoperiodic information) and reproductive axis in male European sea bass, Dicentrarchus labrax. Two seven-day experiments of pinealectomy (Px) were performed, in March (end of reproductive season) and August (resting season)...
February 8, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28185867/an-experimental-evaluation-of-the-role-of-the-stress-axis-in-mediating-predator-prey-interactions-in-wild-marine-fish
#6
Michael J Lawrence, Erika J Eliason, Jacob W Brownscombe, Kathleen M Gilmour, John W Mandelman, Steven J Cooke
The hypothalamic-pituitary-interrenal (HPI) axis, through corticosteroid secretion, is an integral mechanism regulating internal homeostasis when vertebrates are faced with a stressor. However, continued HPI-axis stimulation can produce homeostatic overload, where corticosteroids are detrimental to organismal function. This overload condition may play an important role in mediating predator-prey interactions, because chronically/previously stressed animals may have higher rates of predator-induced mortality...
February 6, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28179140/temperature-dependent-growth-feeding-nutritional-condition-and-aerobic-metabolism-of-juvenile-spiny-lobster-sagmariasus-verreauxi
#7
Quinn P Fitzgibbon, Cedric J Simon, Gregory G Smith, Chris G Carter, Stephen C Battaglene
We examined the effects of temperature on the growth, feeding, nutritional condition and aerobic metabolism of juvenile spiny lobster, Sagmariasus verreauxi, in order to determine if temperature acclimated aerobic scope correlates with optimum for growth and to establish the thermal tolerance window for this emerging aquaculture species. Juvenile lobsters (initial weight=10.95±0.47g) were reared (n=7) at temperatures from 11.0 to 28.5°C for 145days. All lobsters survived from 14.5 to 25.0°C while survival was reduced at 11...
February 5, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28179141/understanding-evolutionary-variation-in-basal-metabolic-rate-an-analysis-in-subterranean-rodents
#8
Facundo Luna, Hugo Naya, Daniel E Naya
Understanding how evolutionary variation in energetic metabolism arises is central to several theories in animal biology. Basal metabolic rate (BMR) -i.e., the minimum rate of energy necessary to maintain thermal homeostasis in endotherms- is a highly informative measure to increase our understanding, because it is determined under highly standardized conditions. In this study we evaluate the relationship between taxa- and mass-independent (residual) BMR and ten environmental factors for 34 subterranean rodent species...
February 4, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28130071/an-energetic-perspective-on-tissue-regeneration-the-costs-of-tail-autotomy-in-growing-geckos
#9
Zuzana Starostová, Lumír Gvoždík, Lukáš Kratochvíl
Tail autotomy is a crucial antipredatory lizard response, which greatly increases individual survival, but at the same time also compromises locomotor performance, sacrifices energy stores and induces a higher burden due to the ensuing response of regenerating the lost body part. The potential costs of tail autotomy include shifts in energy allocation and metabolic rates, especially in juveniles, which invest their energy primarily in somatic growth. We compared the metabolic rates and followed the growth of juvenile males with and without regenerating tails in the Madagascar ground gecko (Paroedura picta), a nocturnal ground-dwelling lizard...
January 24, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28130070/identification-of-the-putative-goldfish-carassius-auratus-magnesium-transporter-slc41a1-and-functional-regulation-in-the-gill-kidney-and-intestine-in-response-to-dietary-and-environmental-manipulations
#10
Vladimir Kodzhahinchev, Drago Kovacevic, Carol Bucking
While magnesium requirements for teleost fish highlight the physiological importance of this cation for homeostasis, little is known regarding the molecular identity of transporters responsible for magnesium absorption or secretion. The recent characterization of the vertebrate magnesium transporter solute carrier 41a1 (SLC41a1) in the kidney of a euryhaline fish has provided a glimpse of possible moieties involved in piscine magnesium regulation. The present study obtained a novel SLC41a1 coding sequence for Carassius auratus and demonstrated ubiquitous expression in all tissues examined...
January 24, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28109839/ex-utero-culture-of-viviparous-embryos-of-the-lizard-zootoca-vivipara-provides-insights-into-calcium-homeostasis-during-development
#11
Tom W Ecay, James R Stewart, Greg Wiessner, Benoit Heulin
The chorioallantoic membrane resides adjacent to either the inner surface of the egg shell or uterine epithelium in oviparous and viviparous reptiles, respectively. Chorionic cells face the shell or uterine epithelium and transport calcium to underlying embryonic capillaries. Calcium transport activity of the chorioallantois increases in the final stages of development coincident with rapid embryonic growth and skeletal ossification. We excised embryos from viviparous Zootoca vivipara females at a stage prior to significant calcium accumulation and incubated them ex utero with and without calcium to test the hypothesis that chorioallantois calcium transport activity depends on developmental stage and not calcium availability...
January 18, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28109838/developmental-changes-and-day-night-expression-of-the-gonadotropin-inhibitory-hormone-system-in-the-european-sea-bass-effects-of-rearing-temperature
#12
José A Paullada-Salmerón, Guillaume Henri Loentgen, Mairi Cowan, María Aliaga-Guerrero, María Del Carmen Rendón-Unceta, José A Muñoz-Cueto
The role of rearing temperature on fish development, sex differentiation and puberty has been largely addressed, but the impact of water temperature on the ontogeny of the main neuroendocrine systems controlling reproduction has received little attention. Gonadotropin-inhibitory hormone (GnIH) has been shown to act on gonadotropin-releasing hormone (GnRH) neurons and on the pituitary to inhibit gonadotropin release and synthesis in vertebrates, including sea bass, Dicentrarchus labrax. In the present study we investigated the effects of rearing temperature during the thermosensitive period (5-60days post-fertilization, dpf) on the expression of the GnIH gene (gnih) and its receptor (gnihr)...
January 18, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28109837/interspecific-variation-and-plasticity-in-hemoglobin-nitrite-reductase-activity-and-its-correlation-with-oxygen-affinity-in-vertebrates
#13
Frank B Jensen, Rasmus A H Kolind, Natashia S Jensen, Gabriella Montesanti, Tobias Wang
Deoxygenated hemoglobin (Hb) is a nitrite reductase that reduces naturally occurring nitrite to nitric oxide (NO), supplying physiological relevant NO under hypoxic conditions. The nitrite reductase activity is modulated by the allosteric equilibrium between the R and T structures of Hb that also determines oxygen affinity. In the present study we investigated nitrite reductase activity and O2 affinity in Hbs from ten different vertebrate species under identical conditions to disclose interspecific variations and allow an extended test for a correlation between the rate constant for nitrite reduction and O2 affinity...
January 18, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28095297/dual-estrogenic-regulation-of-the-nuclear-progestin-receptor-and-spermatogonial-renewal-during-gilthead-seabream-sparus-aurata-spermatogenesis
#14
François Chauvigné, Janmejay Parhi, Judith Ollé, Joan Cerdà
Studies in teleosts suggest that progestins have crucial functions during early spermatogenesis. However, the role of the different progestin receptors in these mechanisms is poorly understood. In this work, we investigated the expression pattern and hormonal regulation of the classical nuclear progestin receptor (Pgr) in the gilthead seabream at three different stages of spermatogenesis: the resting (postspawning) phase, onset of spermatogenesis, and spermiation. Immunolocalization experiments using a seabream specific Pgr antibody revealed that the receptor was expressed in Sertoli and Leydig cells, and also in a subset of spermatogonia type A, throughout spermatogenesis...
January 15, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28089858/glucose-amino-acids-and-fatty-acids-directly-regulate-ghrelin-and-nucb2-nesfatin-1-in-the-intestine-and-hepatopancreas-of-goldfish-carassius-auratus-in-vitro
#15
Juan Ignacio Bertucci, Ayelén Melisa Blanco, Luis Fabián Canosa, Suraj Unniappan
Ghrelin and nesfatin-1 are two peptidyl hormones primarily involved in food intake regulation. We previously reported that the amount of dietary carbohydrates, protein and lipids modulates the expression of these peptides in goldfish in vivo. In the present work, we aimed to characterize the effects of single nutrients on ghrelin and nesfatin-1 in the intestine and hepatopancreas. First, immunolocalization of ghrelin and NUCB2/nesfatin-1 in goldfish hepatopancreas cells was studied by immunohistochemistry. Second, the effects of 2 and 4hour-long exposures of cultured intestine and hepatopancreas sections to glucose, l-tryptophan, oleic acid, linolenic acid (LNA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on ghrelin and nesfatin-1 gene and protein expression were studied...
January 13, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28089857/na-k-atpase-activity-in-the-anoxic-turtle-trachemys-scripta-brain-at-different-acclimation-temperature
#16
Jonathan A W Stecyk, Anthony P Farrell, Matti Vornanen
Survival of prolonged anoxia requires a balance between cellular ATP demand and anaerobic ATP supply from glycolysis, especially in critical tissues such as the brain. To add insight into the ATP demand of the brain of the anoxia-tolerant red-eared slider turtle (Trachemys scripta) during prolonged periods of anoxic submergence, we quantified and compared the number of Na(+)-K(+)-ATPase units and their molecular activity in brain tissue from turtles acclimated to either 21°C or 5°C and exposed to either normoxia or anoxia (6h 21°C; 14days at 5°C)...
January 13, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28089856/erythrocyte-heat-shock-protein-responses-to-chronic-in-vivo-and-acute-in-vitro-temperature-challenge-in-diploid-and-triploid-salmonids
#17
Pillai V Saranyan, Neil W Ross, Tillmann J Benfey
This research investigated how ploidy level (diploid versus triploid) affects the heat shock protein (HSP) response in erythrocytes under different thermal stress regimes, both in vivo and in vitro, in Atlantic salmon (Salmo salar) and brook charr (Salvelinus fontinalis) in order to address the question of why triploids typically have reduced thermal tolerance. A preliminary study confirmed that identical volumes of diploid and triploid erythrocytes (which equates to a smaller number of larger cells for triploids compared to diploids) did not differ in total protein synthesis rates...
January 13, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28087330/molecular-characterization-and-functional-analysis-of-a-salmon-louse-lepeophtheirus-salmonis-kr%C3%A3-yer-1838-heme-peroxidase-with-a-potential-role-in-extracellular-matrixes
#18
Aina-Cathrine Øvergård, Christiane Eichner, Frank Nilsen, Sussie Dalvin
Heme peroxidases are the most abundant type of peroxidase catalyzing a H2O2-dependent oxidation of a wide variety of substrates. They are involved in numerous processes like the innate immune response, hormone and prostaglandin synthesis and crosslinking of proteins within extracellular matrixes (ECM) as well as molecules within the cuticle and chorion of arthropods and nematodes. In the present study, a Lepeophtheirus salmonis heme peroxidase (LsHPX) 1 was characterized. Amino acids in the active site of heme peroxidases were conserved, and the predicted protein sequence showed the highest similarity to genes annotated as chorion peroxidases and genes suggested to be involved in cuticle hardening or adhesion...
January 11, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28087331/different-age-dependent-performance-in-drosophila-wild-type-canton-s-and-the-white-mutant-w1118-flies
#19
Shuang Qiu, Chengfeng Xiao, R Meldrum Robertson
Aging has significant effects on the locomotor performance of insects including Drosophila. Using a protocol for the high-throughput analysis of fly locomotion in a circular arena, we examined age-dependent behavioral characteristics in adult flies. There are widely used wild-type and genetically engineered background lines including the Canton-S strain and the w1118 strain, which has a null mutation of the white gene. Under standard rearing conditions, we found similar survival and median lifespans in Canton-S (50days) and w1118 (54days) strains, however, w1118 flies maintained stable body mass for up to 43days, whereas Canton-S flies gained body mass at young age, followed by a gradual decline...
January 10, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28062221/extreme-intra-clutch-egg-size-dimorphism-is-not-coupled-with-corresponding-differences-in-antioxidant-capacity-and-stable-isotopes-between-eggs
#20
Maud Poisbleau, Michaël Beaulieu, Nina Dehnhard, Laurent Demongin, Gilles Lepoint, Nicolas Sturaro, Marcel Eens
Oviparous females need to allocate resources optimally to their eggs in order to maximize their fitness. Among these resources, dietary antioxidants, acquired by females and transferred to the eggs during egg formation, can greatly affect the development and survival of the embryo and chick. In crested penguins, incubation starts after the second and last egg is laid and, as opposed to many other bird species, this egg hatches first, thereby enhancing the survival of the chick. Here, we assessed whether antioxidant and isotopic composition could underlie these differences between eggs within clutches of southern rockhopper penguins (Eudyptes chrysocome chrysocome)...
March 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
journal
journal
32965
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"