journal
MENU ▼
Read by QxMD icon Read
search

Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology

journal
https://www.readbyqxmd.com/read/28822779/molecular-characterization-of-kiss2-and-differential-regulation-of-reproduction-related-genes-by-sex-steroids-in-the-hypothalamus-of-half-smooth-tongue-sole-cynoglossus-semilaevis
#1
Bin Wang, Quan Liu, Xuezhou Liu, Yongjiang Xu, Xuesong Song, Bao Shi
Kisspeptin (Kiss) plays a critical role in mediating gonadal steroid feedback to the gonadotropin-releasing hormone (GnRH) neurons in mammals. However, little information regarding the regulation of kisspeptin gene by sex steroids is available in teleosts. In this study, we examined the direct actions of estradiol (E2) and testosterone (T) on hypothalamic expression of kisspeptin and other key factors involved in reproductive function of half-smooth tongue sole. As a first step, a partial-length cDNA of kiss2 was identified from the brain of tongue sole and kiss2 transcript levels were shown to be widely expressed in various tissues, notably in the ovary...
August 16, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28807837/characterization-of-two-odorant-binding-proteins-in-spodoptera-exigua-reveals-functional-conservation-and-difference
#2
Nai-Yong Liu, Jia-Yao Zhu, Ting Zhang, Shuang-Lin Dong
As the first biochemical step of olfactory reception and recognition, odorant binding proteins (OBPs) have been demonstrated to be essential. Considering functional diversities of OBPs within a single species, we here extended the characterization of two other OBPs from Spodoptera exigua, belonging to insect Classic OBPs. With a combination of transcriptome and Rapid Amplification of cDNA End (RACE) approaches, two OBP genes in S. exigua were first identified, namely SexiOBP1 and OBP7. Expression pattern analysis revealed that both of them exhibited a distinct expression, where OBP1 was broadly and highly expressed in several tissues including antennae of adults whereas OBP7 was abundant only in the antennae of both sexes, strongly indicative of olfactory roles...
August 11, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28789975/peripheral-neuropeptide-y-differentially-influences-adipogenesis-and-lipolysis-in-chicks-from-lines-selected-for-low-or-high-body-weight
#3
Lingbin Liu, Guoqing Wang, Yang Xiao, Steven L Shipp, Paul B Siegel, Mark A Cline, Elizabeth R Gilbert
Neuropeptide Y (NPY) stimulates appetite and promotes lipid deposition. We demonstrated a differential sensitivity in the food intake response to central NPY in chicks from lines selected for low (LWS) or high (HWS) body weight, but have not reported whether such differences exist in the periphery. At 5days, LWS and HWS chicks were intraperitoneally injected with 0 (vehicle), 60, or 120μg/kg BW NPY and subcutaneous adipose tissue and plasma were collected at 1, 3, 6, 12, and 24h (n=12). NPY injection increased glycerol-3-phosphate dehydrogenase (G3PDH) activity at 1 and 3h and reduced plasma NEFAs at 1 and 12h...
August 5, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28774754/altitudinal-variation-in-metabolic-parameters-of-a-small-afrotropical-bird
#4
Lindy J Thompson, Colleen T Downs
Of the numerous factors affecting avian metabolic rate, altitude is one of the least studied. We used mass-flow respirometry to measure resting metabolic rate (RMR), evaporative water loss (EWL) and respiratory exchange ratio (RER) in two populations of a small (10-12g) Afrotropical bird, the Cape White-eye (Zosterops virens), in summer and in winter. In total, 51 freshly wild-caught adult Cape White-eyes were measured overnight. Altitude was included as a source of variation in the best approximating models for body mass, whole-animal RMR, RER, whole-animal standard EWL and whole-animal basal EWL...
July 31, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28774753/aerobic-scope-in-chicken-embryos
#5
Satoko T Ide, Ryoji Ide, Jacopo P Mortola
We investigated the aerobic scope of chicken embryos, that is, the margin of increase of oxygen consumption ( [Formula: see text] ) above its normal value. [Formula: see text] was measured by an open-flow methodology at embryonic ages E3, E7, E11, E15, E19 and at E20 at the internal (IP) and external pipping (EP) phases, at the normal incubation temperature (Ta=38°C), in hypothermia (Ta=30°C) and in hyperthermia (Ta=41 and 44°C). In the cold, Q10 averaged ~2 at all ages, except in IP and EP when lower values (~1...
July 31, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28764991/transcriptional-translational-and-systemic-alterations-during-the-time-course-of-osmoregulatory-acclimation-in-two-palaemonid-shrimps-from-distinct-osmotic-niches
#6
Rogério Oliveira Faleiros, Rosa P M Furriel, John Campbell McNamara
Palaemonid shrimps exhibit numerous adaptive strategies, both in their life cycles and in biochemical, physiological, morphological and behavioral characteristics that reflect the wide variety of habitats in which they occur, including species that are of particular interest when analyzing adaptive osmoregulatory strategies. The present investigation evaluates the short- (hours) and long-term (days) time courses of responses of two palaemonid shrimps from separate yet overlapping osmotic niches, Palaemon northropi (marine) and Macrobrachium acanthurus (diadromous, fresh water), to differential salinity challenges at distinct levels of structural organization: (i) transcriptional, analyzing quantitative expression of gill mRNAs that encode for subunits of the Na(+)/K(+)-ATPase and V(H(+))-ATPase ion transporters; (ii) translational, examining the kinetic behavior of gill Na(+)/K(+)-ATPase specific activity; and (iii) systemic, accompanying consequent adjustment of hemolymph osmolality...
July 29, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28760555/rhythmicity-and-plasticity-of-digestive-physiology-in-a-euryhaline-teleost-fish-permit-trachinotus-falcatus
#7
Carlo C Lazado, Per Bovbjerg Pedersen, Huy Quang Nguyen, Ivar Lund
Digestive physiology is considered to be under circadian control, but there is little evidence in teleost fish. The present study explored the rhythmicity and plasticity to feeding schedules of enzymatic digestion in a candidate aquaculture fish, the permit (Trachinotus falcatus). The first experiment identified the rhythms of digestive factors throughout the light-dark (LD) cycle. Gastric luminal pH and pepsin activity showed significant daily variation albeit not rhythmic. These dynamic changes were likewise observed in several digestive enzymes, in which the activities of intestinal protease, chymotrypsin and lipase exhibited significant daily rhythms...
July 29, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28756185/behavioural-physiological-and-biochemical-responses-to-aquatic-hypoxia-in-the-freshwater-crayfish-paranephrops-zealandicus
#8
Rebecca J Broughton, Islay D Marsden, Jonathan V Hill, Chris N Glover
Hypoxia resulting from aquatic eutrophication threatens the population health of the New Zealand freshwater crayfish (koura), Paranephrops zealandicus. An integrated study, combining behavioural, physiological and biochemical approaches, was therefore conducted to characterise the tolerance of this species to hypoxia. When provided with a choice between water flows of high or low dissolved oxygen in short-term laboratory assays, crayfish did not preferentially inhabit waters of higher PO2. However, when an aerial refuge was provided and dissolved oxygen was progressively decreased, crayfish emersed at a PO2 of 0...
July 27, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28746844/heart-rate-variability-reveals-that-a-decrease-in-parasympathetic-rest-and-digest-activity-dominates-autonomic-stress-responses-in-a-free-living-seabird
#9
Martina S Müller, Alexei L Vyssotski, Maki Yamamoto, Ken Yoda
The autonomic stress response, often referred to as the 'fight-or-flight' response, is a highly conserved physiological reaction to stress in vertebrates that occurs via a decrease in parasympathetic (PNS) activity, which promotes self-maintenance 'rest and digest' processes, and an increase in sympathetic (SNS) activity, which prepares an animal for danger ('fight-or-flight'). Though the PNS and SNS both innervate most organs, they often control different tissues and functions within those organs (though the pacemaker of the heart is controlled by both)...
July 23, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28732794/glutamine-protects-intestinal-calcium-absorption-against-oxidative-stress-and-apoptosis
#10
Luciana Moine, Gabriela Díaz de Barboza, Adriana Pérez, Mercedes Benedetto, Nori Tolosa de Talamoni
The aim of this study was to investigate whether glutamine (GLN) could block the inhibition of the intestinal Ca(2+) absorption caused by menadione (MEN), and elucidate the underlying mechanisms. To do this, one-month old chicks were divided in four groups: 1) controls, 2) MEN treated, 3) GLN treated and 4) GLN treated before or after MEN treatment. Intestinal Ca(2+) absorption as well as protein expression of molecules involved in the transcellular Ca(2+) pathway were determined. Glutathione (GSH) and superoxide anion and activity of enzymes of the antioxidant system were evaluated...
July 19, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28723584/the-relationship-between-growth-performance-and-metabolic-rate-flexibility-varies-with-food-availability-in-juvenile-qingbo-spinibarbus-sinensis
#11
Ling-Qing Zeng, Lei Wang, Guan-Nan Wang, Yue Zeng, Shi-Jian Fu
Phenotypic flexibility in traits can allow organisms to cope with environmental challenges. However, the ecological consequences (e.g., growth) of SMR flexibility in fish are poorly understood. Juvenile qingbo (Spinibarbus sinensis) were reared individually with two levels of food resources (satiation or limited) with either continuous feeding (CF) or starvation-refeeding (SR). In the CF experiment, SMR increased when individuals were fed either the satiation or limited diets, but no difference was found in average specific growth rate somatic growth (SGR) between the two food availabilities...
July 16, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28711355/characterization-of-the-foxl2-proximal-promoter-and-coding-sequence-from-the-common-snapping-turtle-chelydra-serpentina
#12
Lei Guo, Turk Rhen
Sex is determined by temperature during embryogenesis in snapping turtles, Chelydra serpentina. Previous studies in this species show that dihydrotestosterone (DHT) induces ovarian development at temperatures that normally produce males or mixed sex ratios. The feminizing effect of DHT is associated with increased expression of FoxL2, suggesting that androgens regulate transcription of FoxL2. To test this hypothesis, we cloned the proximal promoter (1.6kb) and coding sequence for snapping turtle FoxL2 (tFoxL2) in frame with mCherry to produce a fluorescent reporter...
July 12, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28711354/the-roles-of-metabolic-thermogenesis-in-body-fat-regulation-in-striped-hamsters-fed-high-fat-diet-at-different-temperatures
#13
Lu-Lu Shi, Wei-Jia Fan, Ji-Ying Zhang, Xiao-Ya Zhao, Song Tan, Jing Wen, Jing Cao, Xue-Ying Zhang, Qing-Sheng Chi, De-Hua Wang, Zhi-Jun Zhao
The metabolic thermogenesis plays important roles in thermoregulation, and it may be also involved in body fat regulation. The thermogenesis of brown adipose tissue (BAT) is largely affected by ambient temperature, but it is unclear if the roles in body fat regulation are dependent on the temperature. In the present study, uncoupling protein 1 (ucp1)-based BAT thermogenesis, energy budget and body fat content were examined in the striped hamsters fed high fat diet (HF) at cold (5°C) and warm (30°C) temperatures...
July 12, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28687414/ovarian-expression-and-localization-of-clathrin-cltc-components-in-cutthroat-trout-oncorhynchus-clarki-evidence-for-cltc-involvement-in-endocytosis-of-vitellogenin-during-oocyte-growth
#14
Hiroko Mizuta, Yuji Mushirobira, Jun Nagata, Takashi Todo, Akihiko Hara, Benjamin J Reading, Craig V Sullivan, Naoshi Hiramatsu
To evaluate potential involvement of clathrin in endocytosis of vitellogenin (Vtg) by teleost oocytes, cDNAs encoding clathrin heavy chain (cltc) were cloned from ovaries of cutthroat trout. Quantitative PCR revealed three types of cltc (cltc-a1, cltc-a2, cltc-b) to be expressed in 10 different tissues including the ovary. The cltc-a1 alone exhibited a significant decrease in ovarian expression during vitellogenesis; this was correlated with a corresponding decrease in transcripts encoding the major Vtg receptor (Vtgr)...
July 4, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28676379/effects-of-nutrient-and-water-restriction-on-thermal-tolerance-a-test-of-mechanisms-and-hypotheses
#15
Katherine A Mitchell, Leigh Boardman, Susana Clusella-Trullas, John S Terblanche
Nutritional deprivation or desiccation can influence thermal tolerance by impacting the insects' ability to evaporatively cool, maintain cell membrane integrity and conduct protective or repair processes. Recovery from chilling is also linked to the re-establishment of iono- and osmo-regulatory homeostasis. Here, using Mediterranean fruit fly (Ceratitis capitata, Diptera: Tephritidae), we manipulated water and nutrient availability to test the mechanistic expectation that changes in whole organism lipid and water content can elicit variation in cold or heat tolerance (scored as chill coma recovery time and heat knockdown time)...
July 1, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28668499/the-difficulty-with-correlations-energy-expenditure-and-brain-mass-in-bats
#16
Brian K McNab, Meike Köhler
Brain mass has been suggested to determine a mammal's energy expenditure. This potential dependence is examined in 48 species of bats. A correlation between characters may be direct or derived from shared correlations with intervening factors without a direct interaction. Basal rate of metabolism in these bats increases with brain mass: large brains are more expensive than small brains, and both brain mass and basal rate increase with body mass. Basal rate and brain mass also correlate with food habits in bats...
June 29, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28647176/mitochondrial-oxidative-phosphorylation-efficiency-is-upregulated-during-fasting-in-two-major-oxidative-tissues-of-ducklings
#17
Pierre-Axel Monternier, Loïc Teulier, Jocelyne Drai, Aurore Bourguignon, Delphine Collin-Chavagnac, Frédéric Hervant, Jean-Louis Rouanet, Damien Roussel
Fasted endothermic vertebrates must develop physiological responses to maximize energy conservation and survival. The aim of this study was to determine the effect of 1-wk. fasting in 5-wk. old ducklings (Cairina moschata) from whole-body resting metabolic rate and body temperature to metabolic phenotype of tissues and mitochondrial coupling efficiency. At the level of whole organism, the mass-specific metabolic rate of ducklings was decreased by 40% after 1-wk. of fasting, which was associated with nocturnal Tb declines and shallow diurnal hypothermia during fasting...
June 21, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28642099/developmental-cardiovascular-physiology-of-the-olive-ridley-sea-turtle-lepidochelys-olivacea
#18
Dane Alan Crossley, Janna Lee Crossley, Camilla Smith, Martha Harfush, Hermilo Sánchez-Sánchez, Mónica Vanessa Garduño-Paz, José Fernando Méndez-Sánchez
Our understanding of reptilian cardiovascular development and regulation has increased substantially for two species the American alligator (Alligator mississippiensis) and the common snapping turtle (Chelydra serpentina) during the past two decades. However, what we know about cardiovascular maturation in many other species remains poorly understood or unknown. Embryonic sea turtles have been studied to understand the maturation of metabolic function, but these studies have not addressed the cardiovascular system...
September 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28629794/uncoupling-effect-of-palmitate-is-exacerbated-in-skeletal-muscle-mitochondria-of-sea-acclimatized-king-penguins-aptenodytes-patagonicus
#19
Benjamin Rey, Claude Duchamp, Damien Roussel
In king penguin juveniles, the environmental transition from a terrestrial to a marine habitat, occurring at fledging, drastically stimulates lipid catabolism and the remodelling of muscle mitochondria to sustain extensive swimming activity and thermoregulation in the cold circumpolar oceans. However, the exact nature of these mechanisms remains only partially resolved. Here we investigated, in vitro, the uncoupling effect of increasing doses of fatty acids in pectoralis muscle intermyofibrillar mitochondria isolated, either from terrestrial never-immersed or experimentally cold water immersed pre-fledging king penguins or from sea-acclimatized fledged penguins...
September 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28629793/the-effects-of-hypoxia-on-active-ionic-transport-processes-in-the-gill-epithelium-of-hyperregulating-crab-carcinus-maneas
#20
Čedomil Lucu, Andreas Ziegler
Effects of hypoxia on the osmorespiratory functions of the posterior gills of the shore crab Carcinus maenas acclimated to 12ppt seawater (DSW) were studied. Short-circuit current (Isc) across the hemilamella (one epithelium layer supported by cuticle) was substantially reduced under exposure to 1.6, 2.0, or 2.5mg O2/L hypoxic saline (both sides of epithelium) and fully recovered after reoxygenation. Isc was reduced equally in the epithelium exposed to 1.6mg O2/L on both sides and when the apical side was oxygenated and the basolateral side solely exposed to hypoxia...
September 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
journal
journal
32965
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"