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Die Pharmazie

Y Yoshida, K Komori, M Aoki, M Sandou, M Takagi, E Uejima
Chemotherapy is among the standard treatments for esophageal cancer. The docetaxel, 5-fluorouracil, and cisplatin (DCF) protocol yields a better response rate than 5-fluorouracil plus cisplatin. However, the incidence of side effects, such as febrile neutropenia and hematologic toxicity, is also significantly high with the DCF protocol. The granulocyte colony-stimulating factor and pegfilgrastim are prophylactically administered to prevent febrile neutropenia. This retrospective study evaluated the efficacy and safety of pegfilgrastim in patients receiving DCF therapy...
October 1, 2018: Die Pharmazie
Y Tominaga, T Aomori, T Hayakawa, D E Morisky, K Takahashi, M Mochizuki
A cross-sectional survey of 358 patients was conducted among type 2 diabetes patients recruited at medical institutions or via an online research company. Medication adherence was measured using the 8-item Morisky Medication Adherence Scale (MMAS-8). Univariate and multivariate regression analyses of glycosylated hemoglobin (HbA1c) were performed in addition to assessing demographic and disease characteristics and MMAS-8. In conclusion, medication adherence as measured by the MMAS-8 score independently contributes to altering the HbA1c level in the range of 1...
October 1, 2018: Die Pharmazie
M P Neininger, P Buchholz, W Kiess, M Siekmeyer, A Bertsche, T Bertsche
Drug incompatibilities can lead to loss of effectiveness of drugs or to increased risk for undesirable effects that can even be life-threatening. Especially children are at high risk. Databases are an important source of information in routine care to avoid incompatibilities. However, they were supposedly developed considering drugs for use in adults. Thus, we analysed to what extent databases are appropriate for the identification of incompatibilities in intravenous (i.v.) drug therapy in paediatric intensive care...
October 1, 2018: Die Pharmazie
T Tachi, A Yoshida, Y Kanematsu, I Sugita, Y Noguchi, T Osawa, M Yasuda, T Mizui, C Goto, H Teramachi
Over-the-counter (OTC) drugs and health foods/supplements are used as means of self-medication with the aim of preventing diseases and maintaining health. No reports have yet addressed the relationship between healthcare systems and self-medication. Here, we carried out a retrospective survey to identify healthcare system factors affecting OTC drug and health food/supplement usage. Patients hospitalized at Gifu Municipal Hospital between October 1, 2014 and March 31, 2015 were given a survey. The items surveyed were age, gender, disease, alcohol intake/smoking status, insurance classification, and medical pharmaceuticals, OTC drugs, and health foods/supplements used immediately before hospitalization...
October 1, 2018: Die Pharmazie
Bo Wu, Xi Chen, Ying Zhou, Pingjun Hu, Dan Wu, Guodong Zheng, Yi Cai
BACKGROUND: Andrographolide (Andro) belongs to the main bioactive ingredients of Andrographis paniculata. Many studies have shown that andro has a variety of pharmacological activities such as anti-inflammatory, anti-bacterial, anti-virus, anti-oxidant, immune regulation and liver protective effects. Moreover andro has been reported to have anticancer activity in multiple types of cancer, including gastric cancer, breast cancer, lung cancer and so on. However, there is no report about the effect of andro on the human NPC cell line C666-1 and the molecular mechanisms of andro-mediated apoptosis in C666-1 cells remain to be clarified...
October 1, 2018: Die Pharmazie
Jinxia Li, Lu Xu, Rui Sang, Yifan Yu, Bingjie Ge, Xuemei Zhang
Astragalus membranaceus Bunge has long been used to improve immune function in traditional Chinese medicine. The total flavonoids of Astragalus (TFA) are the main active components isolated from Astragalus membranaceus Bunge. Our recent study has shown that TFA has in vivo and in vitro immunomodulatory and anti-inflammatory effects; however, its potential mechanisms have not yet been elucidated. The present study aims to confirm the immunomodulatory and anti-inflammatory mechanisms of the action involved. Murine RAW 264...
October 1, 2018: Die Pharmazie
Ting Li, Youran Zhang, Baihui Zhu, Chunfang Wu, Yong Chen
Recent studies have shown that telmisartan (TMS) is effective for the protection against ischemia/brain damage in rat models. However, the specific underlying mechanism is poorly understood. In line with previous results, our data showed that TMS improves CBF and physiological variables, including pH, pCO2, pO2. Through CD31 immunofluorescence staining, reduction of blood vessel density was found in MCAO group, but TMS treatment could enhance the cerebral vascular density in the ischemic area. Meanwhile, TMS treatment could enhance the number of BrdU/lectin double-positive cells...
October 1, 2018: Die Pharmazie
Xianduo Sun, Xiaomin Tang, Quan Yang
An HPLC method for combined quantification of five major constituents (vicenin-1, schaftoside, isoschaftoside, vicenin-3 and isovitexin) of Desmodium styracifolium was developed to evaluate the quality consistency of this medicinal herb. The chromatographic separation was accomplished on an Agilent 5 TC-C18 column (4.6 × 250 mm, 5 μm) with gradient elution using acetonitrile and 0.1 % aqueous formic acid (v/v). The column temperature was 30 °C and the detection wavelength was 272 nm. The chromatographic data were analyzed with a novel systematic quantitative fingerprint method (SQFM)...
October 1, 2018: Die Pharmazie
P A Makoni, S M Khamanga, K Wa Kasongo, R B Walker
A high performance liquid chromatography with electrochemical detection (HPLC-ECD) method for the quantitation of efavirenz (EFV) was developed, since traditional HPLC-UV methods may be inappropriate, given that EFV undergoes photolytic degradation following exposure to UV light. This work describes the use of response surface methodology (RSM) based on a central composite design (CCD) to develop a stability-indicating HPLC method with pulsed ECD in direct current (DC) mode at an applied potential difference and current of +1400 mV and 1...
October 1, 2018: Die Pharmazie
F P Viljoen, J L du Preez, J C Wessels, M E Aucamp
The monitoring of monoamines and their metabolites in CNS samples can be very valuable in pharmaceutical and biomedical research. A specific high performance liquid chromatography, coupled to a coulometric electrochemical detection method, for the assay of monoamines (dopamine, norepinephrine, epinephrine and serotonin) and their metabolites in rat brain tissue samples was developed. The chromatographic separation was achieved on a C8 reversed phase column with a mobile phase consisting of 0.1 M sodium formate buffer, 5 mM sodium 1-heptanesulfonate, 0...
October 1, 2018: Die Pharmazie
P Phattanawasin, U Sotanaphun, J Burana-Osot, N Piyapolrungroj
Andrographolide was subjected to degradation under acidic and basic conditions. Three degradation products including two novel compounds, 8,9-didehydroandrographolide (AG-A) and 15-seco-andrographolide (AG-B2), and a known product, 14-deoxy-11,12-didehydroandrographolide (AG-B1), were isolated by chromatography and identified on the basis of their NMR and MS data. The degradation product, AG-A, may result from the isomerization of andrographolide under acidic conditions whereas the base degradation products, AG-B1 and AG-B2, were formed most likely due to the dehydration of allyl alcohol and the hydrolysis of andrographolide at the lactone ring, respectively...
October 1, 2018: Die Pharmazie
B Lohrer, F Bracher
The "second identification" of drugs in pharmacies and hospital pharmacies affords the availability of easy to perform and robust tests. Besides determination of melting points and simple chromatography (TLC), reliable color and precipitation reactions are common techniques for this purpose. Preferentially, these reactions allow for the identification of typical functional groups in the drugs. Here we present a reaction for identification of carbamate-type drugs, based on the precipitation of barium carbonate upon treating the analytes with barium hydroxide solution at 80 °C...
October 1, 2018: Die Pharmazie
Y Ino, T Tachi, A Ueno, M Otsubo, H Katsuno, Y Noguchi, C Sakai, K Iguchi, C Kawakami, K Fujisaki, H Teramachi
The aim of this study was to clarify the clinic-level factors related to experiences of and attitudes toward collaboration with community pharmacies. We conducted a postal questionnaire survey of all clinics in Gifu, Japan, assessing the experiences and attitudes of representative clinical staff regarding the following activities in collaboration with community pharmacists: regional care meetings/service adjustment meetings, case study conferences, joint workshops/continuing education conferences, community services, information sharing through medical cooperation networks, and accompanying community pharmacists during home care...
September 1, 2018: Die Pharmazie
Deng Guolan, Wang Lingli, He Wenyi, Zhou Wei, Chen Baowei, Bing Sen
This study aimed to evaluate the anti-inflammatory mechanism of neferine, a bisbenzylisoquinoline alkaloid, on the lipopolysaccharide (LPS)-induced inflammation in Human Umbilical Endothelial Cells (HUVECs) and pulmonary aorta cells. Production of pro-inflammatory cytokines and nitric oxide (NO) was determined using Griess reaction in human endothelial cells. The levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and mitogen-activated protein kinases (MAPKs) were analyzed using real time PCR and western blotting...
September 1, 2018: Die Pharmazie
I Cura-Esquivel, E N Delgado-Chávez, J H García-Narro, L Torres-González, G Alarcón-Galván, D P Moreno-Peña, D Esquivel-Figueroa, D V Cantú-Machuca, L E Muñoz-Espinosa, L Garza-Ocañas, P Cordero-Pérez
The ischemia/reperfusion (I/R) process alters metabolic pathways, releasing reactive oxygen species and pro-inflammatory cytokines that cause tissue necrosis and activate cellular apoptotic pathways. Misoprostol (MSP) is a prostaglandin E1 analog that has demonstrated a cytoprotective role in the I/R process. The study objective was to evaluate the effects of MSP on the regulation of pro-inflammatory and oxidative stress mediators in an I/R-induced acute kidney injury rat model. Wistar rats were divided into 3 groups...
September 1, 2018: Die Pharmazie
Fangyi Long, Weixin Liu, Ping Jia, Huafei Wang, Gang Jiang, Ting Wang
In this study, the viability and apoptosis of ovarian cancer cell line OVCAR-3 were assessed using MTT and flow cytometry analysis. GFP-tagged LC3 plasmid transfection was utilized to demonstrate the occurrence of autophagy. The expression of HIF-1α, Beclin1, LC3 and β-actin were determined with Western blot analysis. We found that hypoxia could inhibit cisplatin induced apoptosis in OVCAR-3 cells and enhance the chemoresistance to cisplatin. Furthermore, GFP-tagged LC3 plasmid transfection and western blot were used to demonstrate that hypoxia induced chemoresistance of OVCAR-3 cells to cisplatin is related to HIF-1α-induced autophagy...
September 1, 2018: Die Pharmazie
Qing-Fang Meng, Xin-Xin Zhang, Zheng Zhang, Wei Chen, Xiao-Li Li, Yan-Jun Wang, Fei-Fei Li, Yan-Bin Li
Artesunate is a semi-synthetic derivative of a Chinese herb named Artemisia annua L. that is commonly used as an antimalarial agent in the history of traditional Chinese medicine. Many studies have reported artesunate possesses anti-inflammatory and immunoregulation properties. The present study was conducted to explore whether artesunate was effective in experimental autoimmune myasthenia gravis (EAMG) in Lewis rats. Our data showed that artesunate could improve the clinical symptoms and suppress the development of EAMG...
September 1, 2018: Die Pharmazie
Y Sugino, F Kugawa
Here we searched for microRNAs that could interact with cytochrome P450 (CYP) enzymes in silico, and then investigated their effects on Cyp gene expressions using the cultured mouse liver cell line AML12. Among the mouse Cyp3a genes, some miRNAs were found to interact with Cyp3a11, 13, 16, and 44 by the in silico analysis using the miRWalk2.0 database. In addition to this software, which included twelve miRNA target prediction algorithms, we also applied our in-house-developed Excel VBA algorithm to obtain predictions more efficiently...
September 1, 2018: Die Pharmazie
Zhenglei Xu, Bihong Liao, Ru Zhang, Yingxue Li, Li Mei, Lisheng Wang
This study aimed to investigate the role of microRNA-128-3p in regulating hydrochloric acid (HCl)-induced cell injury in human esophageal squamous Het-1A cells. Het-1A cells were treated with HCl (pH 4.0) to induce esophageal injury like this caused by reflux. Whether HCl induced cell injury was then assessed by detecting cell proliferation and apoptosis. The expression of miR-128-3p in HCl-treated Het-1A cells was investigated, followed by investigating the effects of miR-128-3p overexpression and suppression on HCl-induced Het-1A cell injury...
September 1, 2018: Die Pharmazie
Wei You, Zhiming Wu, Fei Ye, Xiangqi Wu
The mTORC1-dependent signaling pathway is mainly involved in the adverse left ventricular remodeling (ALVR) process after myocardial infarction (MI). However, whether mTORC1 inhibition by cardamonin attenuates ALVR after MI is still not reported. Twenty mice were randomly assigned into three groups: sham group (10 ml/kg/day PBS, n=6), model group (MI and 10 ml/kg/day PBS, n=7) and cardamonin-treated group (MI and 20 mg/kg/day cardamonin, n=7). All groups received an intraperitoneal injection accordingly for two weeks...
September 1, 2018: Die Pharmazie
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