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Journal of Biochemical and Molecular Toxicology

Weidong Jiang, Dacheng Wen, Zhaohua Cheng, Yongsheng Yang, Ge Zheng, Fangying Yin
The target of the current research was to investigate the anticancer activity of sitagliptin on diethylnitrosamine (DENA)-induced cancer in the liver. Wistar rats were treated with or without sitagliptin before DENA treatment. We detected liver weight, blood glucose, and histopathology of the liver. Serum biochemical markers like serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), serum alkaline phosphatase (SALP), gamma-glutamyl transpeptidase (GGTP), total bilirubin (TBR), total protein (TPR), and albumin (ALB) were also evaluated...
September 15, 2018: Journal of Biochemical and Molecular Toxicology
Faik Gökalp
Garlic has been used as a traditional medicine to treat various diseases. Garlic reduces the risk of some diseases. This protective effect is due to the organosulfur compounds of garlic. The aim of this study was to investigate the inhibition effects of garlic-derived compounds on human immunodeficiency virus type 1 (HIV-1) and as the most important anti-HIV-1 medicine. The activation of saquinavir is believed to be the principal mechanism behind the protective effects of HIV-1. Our theoretical calculations are performed for blood phase by using the density functional theory for the main compounds of garlic...
September 8, 2018: Journal of Biochemical and Molecular Toxicology
Mohammed S El-Awady, Eman Said, Hany N Baraka
Acute lung injury (ALI) is a universal cause of respiratory failure and death especially after sepsis. The current study evaluates the protective efficacy of acylated catalpol diglycoside (ACD), a plant iridoid glycoside, against lipopolysaccharides (LPS)-induced ALI in rats. ACD prevented LPS-induced elevations in total and differential cell counts and total protein content in bronchoalveolar lavage fluid, lung malondialdehyde content, and serum lactate dehydrogenase activity. Moreover, ACD significantly increased lung glutathione and superoxide dismutase and improved lung histopathology with significant reduction in lung tumor necrosis factor-α (TNF-α) content mediated mainly via inhibition of TNF-α messenger RNA (mRNA) expression...
September 8, 2018: Journal of Biochemical and Molecular Toxicology
Radhiga Thangaiyan, Beaulah Mary Robert, Sundaresan Arjunan, Kanimozhi Govindasamy, Rajendra Prasad Nagarajan
We investigated the effect of apigenin, a dietary flavonoid, on isoproterenol hydrochloride (ISO)-induced apoptotic signaling in cardiomyoblast H9C2 cells. The results showed that apigenin treatment (10 µM) prevented ISO (31.25 μM)-induced lipid peroxidative levels and antioxidants status in H9C2 cells. Furthermore, apigenin inhibited expression of inflammatory markers in ISO-treated cells. In addition, apigenin prevented ISO-induced DNA damage and apoptotic signaling through modulating the expression of Bax, caspase-3, -8 and -9, cytochrome c, and Fas proteins in H9C2 cells...
August 28, 2018: Journal of Biochemical and Molecular Toxicology
Nahid Ahmadi, Vahid Ghanbarinejad, Mohammad Mehdi Ommati, Akram Jamshidzadeh, Reza Heidari
Brain tissue manganese (Mn) accumulation is a cirrhosis-associated complication. Cellular mitochondria are among the potential targets for Mn-induced cytotoxicity. Taurine is one of the most abundant amino acids with high concentrations in human brain tissue. Several pharmacological properties including regulation of mitochondrial function are attributed to taurine. The current investigation was designed to evaluate the effect of taurine on Mn-induced mitochondrial impairment in isolated mice brain mitochondria...
August 28, 2018: Journal of Biochemical and Molecular Toxicology
Maha G Soliman, Hanaa A Mansour, Wedad A Hassan, Rasha A El-Sayed, Nahla A Hassaan
Inhalation of bacterial endotoxin induces an acute inflammation in the lower respiratory tract. The current study examined the therapeutic effects of bone marrow mesenchymal stem cells (BM-MSCs) in lipopolysaccharide (LPS)-induced pulmonary congestion in rats as compared with dexamethasone (Dexa) and sodium bicarbonate (NaHCO3 ). LPS (20 µL of LPS of Escherichia coli in each nostril for two consecutive days) induced lung injury as marked by an elevation of number of inflammatory cells especially neutrophils, increased total protein levels, elevation of lipid peroxidation, and reduction of reduced glutathione in bronchoalveolar lavage along with the reduction of reduced glutathione...
August 28, 2018: Journal of Biochemical and Molecular Toxicology
Yong Zhang, Xuan Wang, Zhaoguo Liu, Lingzhi Yu
Dexmedetomidine (Dex) is an agonist of α2-adrenergic receptors, and it is used as an anxiety reducing, sedative, and pain medication in clinical. Studies have shown that dexmedetomidine protects against lipopolysaccharide (LPS)-induced acute lung injury; however, the underlying mechanism is still unclear. To investigate, an acute lung injury mouse model was induced by intraperitoneal injection of LPS. Histopathological changes were determined by hematoxylin and eosin staining. Enzyme-linked immunosorbent assay was used to detect cytokines in serum...
August 13, 2018: Journal of Biochemical and Molecular Toxicology
Manickam Kalappan Vanitha, Pandi Anandakumar, Dhanapal Sakthisekaran
Breast cancer is one of the most severe problems in oncology. Taurine is a sulfur-containing amino acid with vital biological functions. The current study was intended to investigate the abnormalities in the expression of apoptosis-associated proteins that lead to the progression of 7,12-dimethyl benz[a]anthracene (DMBA)-induced breast cancer and to expose the protective effect of taurine on it. Rats were induced with DMBA by gastric intubation to induce breast cancer. Breast cancer-bearing animals were posttreated with taurine...
August 13, 2018: Journal of Biochemical and Molecular Toxicology
Isil Ezgi Eryilmaz, Gamze Guney Eskiler, Unal Egeli, Beste Yurdacan, Gulsah Cecener, Berrin Tunca
The aim of the current study was first to investigate cytotoxic activity of usnic acid (UA) on hormone-dependent breast and prostate cancer, and normal cells. Cells were treated with increasing concentrations (25 to 150 µM) of UA for 48 hours and cell viability, quantitative and morphological analysis of cell death, and cell cycle analysis were performed. UA was shown to have selective cytotoxicity on hormone-dependent cancer cells with the IC50 levels of 71.4 and 77.5 µM for MCF7 and LNCaP cells, respectively...
August 13, 2018: Journal of Biochemical and Molecular Toxicology
Fikret Türkan, Zübeyir Huyut, Mehmet Nuri Atalar
The drugs of the class avermectins are antiparasitic agents, which are widely used in medical and agricultural fields, especially in veterinary medicine. The aim of this study was to investigate the inhibitory effects of avermectin derivatives such as abamectin, doramectin, eprinomectin, ivermectin, and moxidectin, which are used for internal and external mammalian parasites. Glutathione S-transferase (GST, E.C. was purified from fresh human erythrocytes. The purification of the GST enzyme was performed separately by affinity chromatography with a yield of 34...
August 8, 2018: Journal of Biochemical and Molecular Toxicology
Sanjay Kumar, Ibrahim O Farah, Paul B Tchounwou
Trisenox (TX) has been used successfully for the treatment of acute promyelocytic leukemia (APL) patients. TX-induced cytotoxicity in APL cells remains poorly understood. In this study, we investigated the molecular mechanism of TX cytotoxicity using APL cell lines. We assessed TX toxicity by quantitatively measuring lactate dehydrogenase levels. Inhibition of cell cycle progression was assessed by confocal microscopy of Ki-67 expression. Apoptosis was evaluated by Western blot analysis of apoptotic proteins expression, immunocytochemistry, and confocal imaging of annexin V and propidium iodide...
August 8, 2018: Journal of Biochemical and Molecular Toxicology
Jian Ying Yang, Yong Fa Zhang, Yuan Xiao Li, Xiang Ping Meng, Jian Feng Bao
To explore the protective mechanism of l-arginine against T-2 toxin-induced oxidative damage in mouse Leydig cells, Leydig cells were isolated and cultured with control, T-2 toxin (10 nM), l-arginine (0.25, 0.5, and 1.0 mM), and T-2 toxin (10 nM T-2 toxin) with l-arginine (0.25, 0.5, or 1.0 mM) for 24 hours. Cells and supernatants were harvested to examine cell viability, activities, and messenger RNA (mRNA) expression of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT), malondialdehyde (MDA) content, and DNA damage...
August 8, 2018: Journal of Biochemical and Molecular Toxicology
Debjani P Mansingh, Sunanda O J, Veeresh Kumar Sali, Hannah R Vasanthi
Ginger (Zingiber officinale Roscoe), a monocotyledonous herb, is widely used as an herbal medicine owing to the phytoconstituents it possesses. In the current study, the quantity of [6]-gingerol, the major phenolic ketone, in the fresh ginger and dried ginger rhizome was found to be 6.11 µg/mg and 0.407 µg/mg. Furthermore, [6]-gingerol was assessed for its antiapoptotic effects in human gastric adenocarcinoma (AGS) cells evidenced by acridine orange/ethidium bromide staining technique and Annexin-V assay...
August 8, 2018: Journal of Biochemical and Molecular Toxicology
Ke Wang, Xue Zhu, Dan Mei, Zhongyang Ding
Anoikis resistance is considered to be an essential prerequisite of tumor metastasis and which is an important mechanism in the metastatic process of gastric cancer (GC). Caveolin-1 (CAV-1), a protein component of caveolae, has been reported to regulate several cancer cell behaviors including anoikis resistance. However, the role of CAV-1 in the acquisition of anoikis resistance in GC cells has never been explored. In this study, we investigated the promoting effect of CAV-1 on anchorage-independent growth and anoikis resistance, and the involvement of the related signaling pathways in such process in SGC-7901 cells...
August 8, 2018: Journal of Biochemical and Molecular Toxicology
Mohamed Fizur Nagoor Meeran, Farah Laham, Hasan Al-Taee, Sheikh Azimullah, Shreesh Ojha
The effect of α-bisabolol on hemodyanimcs, lipid peroxidation, and nonenzymatic antioxidants was evaluated in isoproterenol-induced myocardial infarction in rats. They were pre- and cotreated with α-bisabolol (25 mg/kg body weight) daily for 10 days along with the subcutaneous injection of isoproterenol (85 mg/kg body weight) at an interval of 24 hours for 2 days (9th and 10th days). Increased activities of serum creatine kinase and creatine kinase-MB along with altered levels/concentrations of lipid peroxidation products and nonenzymatic status were observed in the plasma and heart tissues of rats...
August 8, 2018: Journal of Biochemical and Molecular Toxicology
Kani Zilbeyaz, Nashia Stellenboom, Murat Guney, Aykut Oztekin, Murat Senturk
There is a dire need for new treatments for Alzheimer's disease (AD). Principal drugs have reached maturity, and the number of people affected by AD is growing at a rapid rate. After years of research and many clinical trials, only symptomatic treatments are available. An effective disease-modifying drug for AD needs to be discovered. The research presented in this paper aims to facilitate in the discovery of new potential targets that could help in the ongoing AD research. Aryl methanesulfonate derivatives were screened for their acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitory activities...
August 8, 2018: Journal of Biochemical and Molecular Toxicology
Gulnar Gondolova, Parham Taslimi, Ajdar Medjidov, Vagif Farzaliyev, Afsun Sujayev, Mansura Huseynova, Onur Şahin, Bahattin Yalçın, Fikret Turkan, İlhami Gulçin
[Ni(C11 H9 N2 O5 )2 (H2 O)2 ]•3(C3 H7 NO) (1) and [Co(C11 H9 N2 O5 )2 (H2 O)2 ]•3(C3 H7 NO) (2) are synthesized and characterized by elemental analysis, FT-IR spectra, magnetic susceptibility, and thermal analysis. In addition, the crystal structure of Ni(II) complex is presented. Both complexes show distorted octahedral geometry. In 1 and 2, metal ions are coordinated by two oxygen atoms of salicylic residue and two nitrogen atoms of maleic amide residue from two ligands, and two oxygen atoms from two water molecules...
July 25, 2018: Journal of Biochemical and Molecular Toxicology
Muhammet Serhat Özaslan, Yeliz Demir, Mine Aksoy, Ömer Irfan Küfrevioğlu, Şükrü Beydemir
Glutathione-S-transferases (GSTs) have a function in xenobiotic metabolism. They are a significant multifunctional family with a wide variety of catalytic activities. In the current study, we determined in vitro inhibition effects of 2,4-dichlorophenoxyacetic acid dimethylamine salt (2,4-D DMA), haloxyfop-P-methyl, glyphosate isopropylamine, dichlorvos, and λ-cyhalothrin on purified GST. For this purpose, GST were purified from Van Lake fish (Chalcalburnus tarichii Pallas) liver with 29.25 EU mg-1 specific activity and 10...
July 17, 2018: Journal of Biochemical and Molecular Toxicology
Dehong Chen, Xiaosong Sun, Xuejun Zhang, Jun Cao
Targeting mitochondria respiration is an effective therapeutic strategy in renal cell carcinoma (RCC). Atovaquone is a FDA-approved antibiotic but is also known as a mitochondrial inhibitor. We found that atovaquone inhibited proliferation and induced apoptosis of RCC cells. Mechanistically, atovaquone inhibits mitochondrial respiration in a concentration-dependent and time-dependent manner, via targeting mitochondrial respiratory complex III. Although increased glycolysis was observed in atovaquone-treated cells, atovaquone decreased ATP levels...
July 13, 2018: Journal of Biochemical and Molecular Toxicology
Nahed M A Hassanein, Eman S G Hassan, Amany M Hegab, Helmy M Sayd Ahmed
A systematic investigation of the chemopreventive effect of sulindac (SL) in combination with either epigallocatechin gallate (EGCG) or kaempferol similar (KMP) has been carried out 1,2-dimethyl hydrazine-treated rats (DMH). Those SL combinations with KMP and EGCG have enhanced the SL activity producing greater antioxidant, anti-inflammatory, antiproliferating, and apoptotic activities in both combinations than SL alone. The chemopreventive effects of SL with both EGCG and KMP were demonstrated by a decrease in thiobaribituric acid reactive substances level, tissue nitric oxide (NO), serum, and tissue β-catenin as well as a reduction in the multiplicity of aberrant crypt foci (ACF) with alleviation in the dysplastic changes that resulted from DMH administration...
July 12, 2018: Journal of Biochemical and Molecular Toxicology
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