journal
MENU ▼
Read by QxMD icon Read
search

Journal of Biochemical and Molecular Toxicology

journal
https://www.readbyqxmd.com/read/27925690/purification-and-characterization-of-a-platelet-aggregation-inhibitor-and-anticoagulant-cc-5_ntase-cd-73-like-from-cerastes-cerastes-venom
#1
Samah Saoud, Fatah Chérifi, Traki Benhassine, Fatima Laraba-Djebari
The present study is the first attempt to report the characterization of a nucleotidase from Cerastes cerastes venom. A 70 kDa 5'-nucleotidase (Cc-5'NTase) was purified to homogeneity. The amino acid sequence of Cc-5'NTase displayed high homology with many nucleotidases. Its activity was optimal at pH 7 with a specific hydrolytic activity toward mono-, di-, and triphosphate adenylated nucleotides. Cc-5'NTase preferentially hydrolyzed ADP and obeyed Michaelis-Menten kinetics. Among the metals and inhibitors tested, Ni(2+) and Mg(2+) completely potentiated enzyme activity, whereas EGTA, PMSF, iodoacetamide, vanillic acid, vanillyl mandelic acid, and 1,10-phenanthroline partially abolished its activity...
December 7, 2016: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/27918128/mulberroside-a-suppresses-pxr-mediated-transactivation-and-gene-expression-of-p-gp-in-ls174t-cells
#2
Yuhua Li, Ling Huang, Jiahong Sun, Xiaohua Wei, Jinhua Wen, Guoping Zhong, Min Huang, Huichang Bi
Mulberroside A (Mul A) is the main bioactive constituents of Sangbaipi, which is officially listed in the Chinese Pharmacopoeia. The pregnane X receptor (PXR) has been recognized as the critical mediator of human P-glycoprotein (P-gp) gene transactivation. In this study, the effect of Mul A on PXR-mediated transactivation and gene expression of P-gp was investigated. It was found that Mul A significantly suppressed PXR-mediated P-gp luciferase activity induced by rifampicin (Rif). Furthermore, Rif induced an elevation of P-gp expression and transport activity, which was apparently suppressed by Mul A...
December 5, 2016: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/27902864/in-vitro-and-in-vivo-metabolic-activation-of-berbamine-to-quinone-methide-intermediate
#3
Yao Sun, Tong Yao, Hui Li, Ying Peng, Jiang Zheng
Berbamine (BBM) is a bisbenzylisoquinoline alkaloid isolated from herbal medicine Berberis amurensis. BBM has been widely used for the treatment of leukemia. Recent studies demonstrated that exposure to BBM can give rise to cytotoxicity. The major objective of this study was to explore the metabolic activation of BBM in vitro and in vivo. Two oxidative metabolites (M1 and M2) and an N-acetylcysteine (NAC) conjugate (M3) were detected in human liver microsomal incubations of BBM supplemented with NAC, and the formation of all metabolites was NADPH dependent...
November 30, 2016: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/27900820/investigation-of-the-effects-of-kisspeptin-10-in-methionine-induced-lipid-peroxidation-in-testicle-tissue-of-young-rats
#4
Hatice Akkaya, Signem Eyuboglu, Gozde Erkanlı Senturk, Bayram Yilmaz
Disruption of the balance oxidants, antioxidants cause various pathophysiological conditions such as lipid peroxidation, protein degradation, or DNA damage. We have examined possible effects of kisspeptin-10 on the structural damage produced by methionine-induced lipid peroxidation in testicle tissue of young rats. Kisspeptin-10 did not significantly affect spermatogenic cells in seminiferous tubules. Testosterone levels decreased in the methionine group as compared with the control group but without statistical significance...
November 30, 2016: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/27900814/effects-of-intratracheal-exposure-of-2-chloroethyl-ethyl-sulfide-cees-on-the-activation-of-ccaat-enhancer-binding-protein-c-ebp-and-its-protection-by-antioxidant-liposome
#5
Somdutta Sinha Roy, Shyamali Mukherjee, Salil K Das
Exposure of 2-chloroethyl ethyl sulfide (CEES) to guinea pigs causes lung injury by infiltration of neutrophils in interstitial lung spaces. A unique MAPK-regulated transcription factor, C/EBP (CCAAT-enhancer-binding protein), regulates the expression of intracellular adhesion molecule-1 (ICAM-1), involved in recruiting neutrophils in lung. The present study was to determine if CEES exposure causes activation of C/EBP, in particular the predominant β-isoform and if so whether it can be prevented by intratracheal delivery of an antioxidant liposome containing N-acetyl cysteine and tocopherols...
November 30, 2016: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/27900802/therapeutic-potential-of-carfilzomib-an-irreversible-proteasome-inhibitor-against-acetaminophen-induced-hepatotoxicity-in-mice
#6
Abdulrazaq Alanazi, Saleh G Algfeley, Khaled A Al-Hosaini, Hesham M Korashy, Faisal Imam, Mahmoud N Nagi
Overdose of acetaminophen (APAP) is often associated with hepatotoxicity. Carfilzomib (CFZ) shows multiple pharmacological activities including anti-inflammatory potential. Therefore, this study was undertaken to evaluate the possible therapeutic effects of CFZ against APAP-induced hepatotoxicity. Hepatotoxicity was induced by administration of APAP (350 mg/kg, intraperitoneal). Mice were given CFZ (0.125, 0.25, or 0.5 mg/kg, intraperitoneal) 1.5 h after APAP administration. Animals were sacrificed on 6 h and blood and liver tissue samples were collected for analysis...
November 30, 2016: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/27891704/isoflavone-genistein-inhibits-estrogen-induced-chloride-and-bicarbonate-secretory-mechanisms-in-the-uterus-in-rats
#7
Asma Chinigarzadeh, Kamarulzaman Karim, Sekaran Muniandy, Naguib Salleh
We hypothesized that genistein could affect the chloride (Cl(-) ) and bicarbonate (HCO3(-) ) secretory mechanisms in uterus. Ovariectomized female rats were given estradiol or estradiol plus progesterone with 25, 50, or 100 mg/kg/day genistein. Following completion of the treatment, uterine fluid Cl(-) and HCO3(-) concentrations were determined by in vivo uterine perfusion. Uteri were subjected for molecular biological analysis (Western blot, qPCR, and immunohistochemistry) to detect levels of expression of Cystic Fibrosis transmembrane regulator (CFTR), Cl(-) /HCO3(-) exchanger (SLC26a6), Na(+) /HCO3(-) cotransporter (SLC4a4), and estrogen receptor (ER)-α and β...
November 28, 2016: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/27879033/regulation-of-the-placental-bcrp-transporter-by-ppar-gamma
#8
Yixin Lin, Kristin M Bircsak, Ludwik Gorczyca, Xia Wen, Lauren M Aleksunes
Identifying regulators of placental breast cancer resistance protein (BCRP) expression is critical as downregulation of this transporter may increase exposure of the fetus to xenobiotics. Here, we sought to test whether the nuclear receptor peroxisome proliferator-activated receptor γ (PPARγ) regulates BCRP expression in the placenta. To test this, human BeWo placental choriocarcinoma cells were cultured with the PPARγ agonist rosiglitazone or the PPARγ antagonist T0070907 for 24 h. Messenger RNA (mRNA) expression of syncytialization markers, GCM1 and hCGβ, as well as BCRP increased with PPARγ agonist treatment...
November 23, 2016: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/27879026/ionizing-radiation-promotes-ccl27-secretion-from-keratinocytes-through-the-cross-talk-between-tnf-%C3%AE-and-ros
#9
Qian Zhang, Linlin Zhu, Gang Wang, Ye Zhao, Na Xiong, Hegang Bao, Wensen Jin
The skin-associated chemokine CCL27 and its receptor CCR10 mediate the immune response of skin-homing T cells. The CCL27 secreted from keratinocytes was reportedly involved in inflammatory skin diseases such as atopic dermatitis, contact dermatitis, and psoriasis. However, whether ionizing radiation increases the levels of CCL27 secretion still remains unclear. In HaCaT cells, a human keratinocyte cell line, CCL27 secretion was markedly increased after X-ray irradiation. We further found that irradiation boosted the generation of reactive oxygen species (ROS), which was concomitant with the release of tumor necrosis factor-alpha (TNF-α)...
November 23, 2016: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/27870266/potential-urinary-biomarkers-of-nephrotoxicity-in-cyclophosphamide-treated-rats-investigated-by-nmr-based-metabolic-profiling
#10
Sa Rang Lim, Sun-Hee Hyun, Seul Gi Lee, Jin-Young Kim, So-Hyun Kim, Sang-Jin Park, Kyoung-Sik Moon, Donggeun Sul, Dong Hyun Kim, Hyung-Kyoon Choi
The anticancer-drug cyclophosphamide (CP) is known to have nephrotoxicity. The aim of this study was to identify urinary biomarkers indicating CP-induced nephrotoxicity. We investigated the urine metabolic profiles using nuclear magnetic resonance spectrometry of rats administered with single high-doses of CP (0, 30, and 100 mg/kg body weight) and daily low-doses over a 4-week period (0, 1, 3, and 10 mg/kg body weight). Among 18 identified urinary metabolites, 2-oxoglutarate, citrate, hippurate, formate, valine, and alanine for short-term and 2-oxoglutarate, citrate, hippurate, isoleucine, leucine, allantoin, valine, and lysine for long-term were selected as potential biomarkers...
November 21, 2016: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/27870162/galectin-3-contributes-to-vascular-fibrosis-in-monocrotaline-induced-pulmonary-arterial-hypertension-rat-model
#11
Xiaoyan Wang, Yanli Wang, Jinbo Zhang, Xue Guan, Minggang Chen, Yumei Li, Li Zhang
Galectin-3 (Gal-3) plays a critical role in vascular inflammation and fibrosis. The role of TGF-β1 in mediating pulmonary vascular fibrosis is well documented; thus, we suspected that Gal-3 could be an important factor in TGF-β1-induced fibrosis in pulmonary adventitial fibroblasts (PAFs). We treated rats with monocrotaline (MCT) and cultured PAFs with TGF-β1 to stimulate fibrosis. We found that MCT injection induced vessel thickening and extracellular matrix deposition in vivo. TGF-β1 stimulated the production of collagen and fibronectin (Fn) protein in vitro...
November 21, 2016: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/27862621/exploration-of-zea-cytotoxicity-to-mouse-endometrial-stromal-cells-and-rna-seq-analysis
#12
Haiqiang Xie, Jin Hu, Cheng Xiao, Yujian Dai, Xiaolin Ding, Yinxue Xu
Because zearalenone (ZEA) causes harmful influence to animals and widely exists in the world, the researches on ZEA have never stopped. However, the mechanisms of ZEA on proliferation, cycle, and apoptosis in endometrial stromal cells (ESCs) remain poorly defined. Therefore, the purpose of our study was to explore the effects of ZEA to ESCs and demonstrate them by transcriptomic analysis. The results showed that after ZEA treatment, ESCs appeared numerous adverse reactions, and the phenomena of cell viability decrease, DNA replication block and apoptosis were detected by flow cytometry, Annexin V-FITC/PI double-staining method, TUNEL assay, and so on...
November 9, 2016: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/27862620/the-prognostic-value-of-t-lymphoma-invasion-and-metastasis-1-tiam1-expression-in-oral-squamous-cell-carcinoma
#13
Lu Lin, Jingmin You, Yunmei Qian, Ying Han, Haofeng Xiong, Tengfei Zhu, Kun Xia, Tong Su
Oral squamous cell carcinoma (OSCC) is the most common malignant neoplasm with high rate of lymph node metastasis and recurrence. The aim of this study is to evaluate the expression profile of T Lymphoma Invasion and Metastasis 1 (TIAM1) in OSCC and its correlation with clinical outcomes including lymph node metastasis and recurrence. Detection of TIAM1 expression in tumor samples from 56 OSCC patients and oral mucosa samples from 20 healthy people was performed by immunohistochemistry. Then immunostaining score of each patient was calculated...
November 9, 2016: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/27780314/mir-192-5p-mediates-hypoxia-reoxygenation-induced-apoptosis-in-h9c2-cardiomyocytes-via-targeting-of-fabp3
#14
Yuefeng Zhang, Risheng Huang, Weihe Zhou, Qifeng Zhao, Zhenye Lü
Myocardial ischemia/reperfusion (I/R) injury is a leading cause of morbidity and mortality. In this study, we investigated the role of miR-192-5p in hypoxia/reoxygenation (H/R)-induced cardiomyocyte apoptosis. H9c2 cardiomyocytes were subjected to H/R and tested for miR-192-5p expression. Overexpression and knockdown experiments were performed to determine the effects of manipulating miR-192-5p on apoptotic responses. H/R-treated H9c2 cells exhibited a 2.2-fold increase in miR-192-5p levels. Overexpression of miR-192-5p significantly augmented apoptosis in H9c2 cells after H/R, which was accompanied by a significant increase in the ratio of Bax/Bcl-2...
October 25, 2016: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/27780313/synthesis-carbonic-anhydrase-i-and-ii-isoenzymes-inhibition-properties-and-antibacterial-activities-of-novel-tetralone-based-1-4-benzothiazepine-derivatives
#15
Mustafa Ceylan, Umit M Kocyigit, Necibe Canan Usta, Belma Gürbüzlü, Yusuf Temel, Saleh H Alwasel, İlhami Gülçin
Benzothiazepine compounds have a wide range of applications such as antibacterial, antidepressants, anticonvulsants, antihypertensives, antibiotics, antifungal, hypnotic, enzyme inhibitors, antitumor, anticancer and anti-HIV agents. In this study, the synthesis of novel tetralone-based benzothiazepine derivatives (1-16) and their in vitro antibacterial activity and human carbonic anhydrase isoenzymes I and II (hCA I and II) inhibitory effects were investigated. Both isoenzymes were purified by sepharose-4B-l-tyrosine-sulfanilamide affinity chromatography from fresh human red blood cells...
October 25, 2016: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/27762473/oxidative-damage-of-the-male-reproductive-system-induced-by-paraquat
#16
Qing Chen, Xin Zhang, Jin-Yan Zhao, Xiao-Ning Lu, Peng-Sheng Zheng, Xiang Xue
The effects of paraquat (PQ) on the male reproductive system are unclear. In this study, male rats were divided into four groups (0, 0.5, 2, and 8 mg/kg) and treated with PQ by oral gavage for 8 weeks. At the end of the experiment, a significant decline in sperm count, motility, and viability and an increase in teratospermia were observed in the PQ-treated group (P < 0.05). Further investigation found that PQ resulted in enhanced lipid peroxidation and more apoptosis in the testis tissues, and apoptosis was likely to be associated with activation of the mitochondrial pathway...
October 20, 2016: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/27762466/hepatoprotective-role-of-vardenafil-against-experimentally-induced-hepatitis-in-mice
#17
Nishat Ahmed, Nada F Bakhashwain, Alaa F Alsehemi, Dina S El-Agamy
Vardenafil is a selective phosphodiesterase-5 inhibitor used for erectile dysfunction treatment. The hepatoprotective role of vardenafil against acute hepatitis is not reported yet. Hence, this study aims to explore the protective role of vardenafil against concanavalin A (Con A) induced acute liver injury. Mice were pretreated with vardenafil (0.17 mg/kg/day) for seven consecutive days, and then subjected to a single IV injection of Con A. The results demonstrated that the vardenafil pretreatment significantly reduced the elevated serum levels of transaminases and alkaline phosphatase...
October 20, 2016: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/27762462/the-binding-properties-of-metandienone-and-human-serum-albumin-by-comparing-with-other-five-similar-compounds
#18
Xiang Lin, Qing Wang, Xilin Peng, Hui Li
Metandienone (MET) is an exogenous anabolic androgenic steroid. The interaction between MET and human serum albumin (HSA) was investigated by molecular modeling and different optical techniques. There was no possibility of energy transfer, and the fluorescence quenching of HSA induced by MET was mainly due to the complex formation. The differences of binding ability between MET and compounds 1-5 were significantly caused by space steric hindrance. The single crystallographic data of two steroids (compounds 4 and 5) were obtained in the methanol at the first time...
October 20, 2016: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/27762461/restoration-of-autophagy-by-puerarin-in-lead-exposed-primary-rat-proximal-tubular-cells-via-regulating-ampk-mtor-signaling
#19
Xiangbin Song, Zifa Li, Fei Liu, Zhenyong Wang, Lin Wang
Previous study has demonstrated that puerarin (PU) exerts nephroprotective effect against Pb-induced cytotoxicity in primary rat proximal tubular (rPT) cells. Autophagy can protect cells from various cytotoxic stimuli, but its role in the process of PU against Pb-induced nephrotoxicity is still unknown. This study aims to investigate whether PU can alleviate Pb-induced renal damage by recovering autophagy. Data showed that Pb inhibited the autophagic flux, as evidenced by the accumulation of LC3-II and p62 as well as the confocal microscopy analysis of GFP-LC3 puncta and punctate spots of monodansylcadaverine staining, whereas coadministration of PU could restore Pb-induced autophagy inhibition...
October 20, 2016: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/27712037/in-vivo-cytochrome-p450-activity-alterations-in-diabetic-nonalcoholic-steatohepatitis-mice
#20
Hui Li, John D Clarke, Anika L Dzierlenga, John Bear, Michael J Goedken, Nathan J Cherrington
Nonalcoholic steatohepatitis (NASH) has been identified as a source of significant interindividual variation in drug metabolism. A previous ex vivo study demonstrated significant changes in hepatic Cytochrome P450 (CYP) activity in human NASH. This study evaluated the in vivo activities of multiple CYP isoforms simultaneously in prominent diabetic NASH mouse models. The pharmacokinetics of CYP selective substrates: caffeine, losartan, and omeprazole changed significantly in a diabetic NASH mouse model, indicating attenuation of the activity of Cyp1a2 and Cyp2c29, respectively...
October 6, 2016: Journal of Biochemical and Molecular Toxicology
journal
journal
32894
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"