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Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology

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https://www.readbyqxmd.com/read/28316431/on-the-recovery-of-horsehair-worms-gordius-sp-nematomorpha-gordiida-from-pork-in-shillong-india
#1
Arun K Yadav, Andreas Schmidt-Rhaesa, R Laha, A Sen
The members of the phylum Nematomorpha, called as horsehair or gordian worms, are the parasites of arthropods and emerge from their host for reproduction and early development in water. There are about 360 species of nematomorphs described till date. Although, horsehair worms are parasites of arthropod species, sporadic cases of their occurrence have also been reported from several other hosts, including man. This paper describes a case history of the occurrence of two nematomorph worms in the meat of a pig in Shillong, India...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28316430/comparison-of-three-cost-effective-staining-methods-for-detection-of-pneumocystis-jirovecii
#2
Mohamad Mohsen Homayouni, Ali Rostami, Hamed Gholizadeh, Amir Sayed Ali Mehbod, Maryam Ebrahimi, Saeed Mehravar
Pneumocystis pneumonia due to Pneumocystis jirovecii infection is an emerging health problem not only for HIV-infected patients but also for other immunocompromised patients in many countries. We compared Gomori methenamine silver (GMS), Toluidine Blue O (TBO) and Giemsa staining methods using standard procedures. The sensitivity and specificity of GMS were 100 %. The sensitivity and specificity of TBO were 96 and 100 %, respectively. The sensitivity and specificity of Giemsa stain were 84 and 90 %, respectively...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28316429/pseudomalaria-in-a-domestic-pigeon-a-case-report
#3
B R Maharana, Binod Kumar
Microscopic examination of thin blood smear of a domestic pigeon brought to Teaching Veterinary Clinical Complex, Junagadh, Gujarat revealed mature and immature halter shaped gametocytes of Haemoproteus columbae encircling the nucleated erythrocytes. The disease was diagnosed as acute infection of pigeon malaria more notably known as pseudomalaria. The parasitic infection was further confirmed by polymerase chain reaction assay targeting the Cytochrome b (cyt b) gene fragment amplifying 207 base pair (bp) fragment...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28316428/first-report-of-seuratascaris-numidica-seurat-1917-nematoda-ascarididae-and-other-helminths-from-amphibians-of-west-bengal-india
#4
Sujan K Sou, Charles R Bursey
Studies on nematode parasites from anuran hosts from Eastern India, particularly West Bengal, are rare. To our knowledge, there is a report of larvae of Monhysterides sp. (a fish parasite) from cysts within the body cavity and musculature of Duttaphrynus melanostictus, Hoplobatrachus tigerinus and Euphlyctis hexadactylus as well as descriptions of Aplectana duttaphryni from D. melanostictus, Aplectana dubrajpuri from H. tigerinus, Cosmocerca microhylae from Microhyla rubra and Rhabdias bulbicauda from D. melanostictus...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28316427/evaluation-of-toxoplasma-gondii-soluble-whole-and-excretory-secretary-antigens-for-diagnosis-of-toxoplasmosis-by-elisa-test
#5
S Pishkari, S Shojaee, H Keshavarz, M Salimi, M Mohebali
The present study was performed to compare the soluble, whole and excretory/secretary antigens of Toxoplasma gondii (RH strain) in diagnosis of toxoplasmosis by ELISA method. Tachyzoites of T. gondii, RH strain were injected in intra-peritoneal cavity of BALB/c mice, after 4 days tachyzoites were harvested by peritoneal washing of the mice. For soluble antigen, exudates were centrifuged and sediment sonicated and then centrifuged at 4 °C, 1 h, supernatant collected and density of protein determined by Bradford method...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28316426/genotyping-of-benzimidazole-resistant-and-susceptible-isolates-of-haemonchus-contortus-from-sheep-by-allele-specific-pcr
#6
Karthik Mohanraj, Subhra Subhadra, Aravindan Kalyanasundaram, Manikkavasagan Ilangopathy, Muthusamy Raman
Extensive and indiscriminate use of the benzimidazole class of drugs has led to the onset of anthelmintic resistance. In tropical countries like India, Haemonchus contortus is the most pathogenic parasite infecting sheep and goats. The widespread presence of resistant helminths (especially H. contortus) threatens the livestock farming. The use of various drugs has led to single nucleotide polymorphism that causes specific amino acid substitutions in β-tubulin protein of H. contortus to confer resistance. This emphasizes the need for a survey on the present status of resistance in India...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28316425/molecular-characterization-of-leishmania-parasites-isolated-from-sandflies-species-of-a-zoonotic-cutaneous-leishmaniasis-in-musiyan-south-west-iran
#7
Farzaneh Kavarizadeh, Shahram Khademvatan, Babak Vazirianzadeh, Mohammad Hossein Feizhaddad, Mehdi Zarean
Cutaneous leishmaniasis (CL) is vector borne parasitic disease, considered as public health problem especially in border of Iran and Iraq, Dehloran County (Musian district). The aim of this study was molecular identification of Leishmania parasites in sandfly as vectors of Leishmaniasis. Totally 280 female sandflies were trapped by sticky traps from 7 rural areas of Musiyan in September-November 2012. All sandflies were identified using morphological characters of the head and abdominal terminalia. DNA was extracted from female sandflies and Leishmania was identified using PCR and sequencing...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28316424/ovicidal-effect-of-chitinase-and-protease-enzymes-produced-by-soil-fungi-on-the-camel-tick-hyalomma-dromedarii-eggs-acari-ixodidae
#8
Salwa M Habeeb, Heba M Ashry, Moataza M Saad
The aim of this study was to evaluate the effect of chitinase and protease enzymes produced by environmentally safe soil Fungi; Aspergillus sp. NRC 4/5H; Mucor sp. NRC 5; Trichoderma sp. NRC 4/56; Aspergillus sojae; Mucor ranosisinus; Aspergillus oryzae on embryo development of the camel tick Hyalomma dromedarii eggs. The experiment was carried out on the 7 day aged eggs. Concentrations of the chitinase and protease crude enzymes [1:1, 1:2, 1:3, 1:4 and 1:5 (v/v)] were prepared from six stock solutions whose concentrations were 5 units/ml each...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28316423/comparison-and-evaluation-of-four-methods-for-extracting-dna-from-giardia-duodenalis-cysts-for-pcr-targeting-the-tpi-gene
#9
Akram Sepahvand, Nader Pestehchian, Hossein Ali Yousefi, Reza Pahlavan Gharehbaba
Giardia duodenalis is an intestinal flagellated protozoan and the common cause of gastrointestinal diseases in human. This parasite can be seen in two different forms in its life cycle including as cyst and trophozoite. Due to presence of resistant cyst wall, DNA extraction inhibitors along with artifact in stool specimens, this study was performed aiming to evaluate four methods for DNA extraction from G. duodenalis cysts. Seventy G. duodenalis positive stool specimens that were confirmed by light microscope were included in this study...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28316422/validation-of-romanowsky-staining-as-a-novel-screening-test-for-the-detection-of-faecal-cryptosporidial-oocysts
#10
A P S Brar, N K Sood, L D Singla, P Kaur, K Gupta, B S Sandhu
Cryptosporidiosis is an emerging waterborne protozoan disease and one of the major causes of neonatal diarrhea in humans and animals. But the disease remains under diagnosed due to lack of availability of special stains in majority of laboratories at primary health centers. Therefore, it requires a rapid screening test for routine diagnosis in conventional laboratory set up. In this pursuit, the present study was planned. During this study, fecal samples from 100 representative animals randomly selected from 17 out breaks of bovine calf diarrhea, were stained with modified Ziehl Neelsen staining (mZN) and Leishman's stain to demonstrate cryptosporidial oocysts and for routine fecal examination, respectively...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28316421/immunophenotyping-of-blood-mononuclear-cells-from-p-berghei-nk-65-infected-and-immunized-balb-c-mice
#11
Vineet Kumar, Aruna Rakha, Ruchika Saroa, Upma Bagai
The present study demonstrates the alterations in the frequencies of helper, cytotoxic and suppressor T cells in blood of P. berghei infected and TPA immunized rodent host towards the induction of protective immune response. Statistically significant (p < 0.005) number of CD4(+) T cells was recorded on D9 [Post Challenge (PC)] meanwhile CD4(+) Treg cells were found to be declined in immunized mice as compared to infected. This increase in frequencies of T helper cells points towards the stimulation of strong Th2 immune response in immunized mice...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28316420/lb-broth-lyophilized-rabbit-serum-llr-as-a-new-and-suitable-culture-medium-for-cultivation-of-promastigotes-of-leishmania-major
#12
Vahid Nasiri, Abdolhossein Dalimi, Fatemeh Ghaffarifar
Fetal calf serum is the major part and the most expensive ingredient of the Leishmania culture media. Here, the efficacy of the LB broth-lyophilized Rabbit serum medium (LLR) was evaluated in cultivation of Leishmaniamajor. Conventional Luria-Bertani (LB) broth medium was prepared and autoclaved for 15 min at 121 °C and then lyophilized Rabbit serum was added at the 1, 2.5, 5 and 10 % final concentrations. The efficacy of medium was evaluated by assessing the growth ability and replication pattern of the promastigotes of L...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28316419/comparison-between-conventional-and-molecular-methods-for-diagnosis-of-bovine-babesiosis-babesia-bovis-infection-in-tick-infested-cattle-in-upper-egypt
#13
Amira A T Al-Hosary
Ticks and tick-borne diseases are the main problems affecting the livestock production in Egypt. Bovine babesiosis has adverse effects on the animal health and production. A comparison of Giemsa stained blood smears, polymerase chain reaction (PCR) and nested PCR (nPCR) assays for detection of Babesia bovis infection in Egyptian Baladi cattle (Bos taurus) in reference to reverse line blot was carried out. The sensitivity of PCR and nested PCR (nPCR) assays were 65 and 100 % respectively. Giemsa stained blood smears showed the lowest sensitivity (30 %)...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28316418/seroepidemiology-of-toxoplasma-gondii-infection-in-pregnant-women-in-west-iran-determined-by-elisa-and-pcr-analysis
#14
Gholam Reza Ahmadpour, Behrouz Ezatpour, Ramtin Hadighi, Hormoz Oormazdi, Lame Akhlaghi, Fatemeh Tabatabaei, Mehdi Azami, Mozafar Mohammadi Nejad, Hossein Mahmoudvand
Congenital toxoplasmosis can lead to severe damage for the fetus and newborn. Considering that the seroepidemiology of Toxoplasma infection in the pregnant women is poorly studied in west of Iran, the main objective of this study was to estimate the seroprevalence and potential risk factors for congenital toxoplasmosis in Delfan, Iran. In this cross-sectional study, the serum samples obtained from pregnant women who were referred to health centers for routine monitoring of the pregnancy. Totally, 264 sera were screened for IgG and IgM anti-T...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28316417/molecular-characterization-and-phylogenetic-analysis-of-plasmodium-vivax-plasmodium-falciparum-plasmodium-ovale-plasmodium-malariae-and-plasmodium-cynomolgi
#15
Soumendranath Chatterjee, Priyanka Mukhopadhyay, Raktima Bandyopadhyay, Paltu Dhal, Debraj Biswal, Prabir Kumar Bandyopadhyay
18S ribosomal RNA gene sequences of different species of Plasmodium were aligned and analyzed to determine the molecular diversity among different species of Plasmodium. AT content of P. cynomolgi, P. ovale, P. falciparum, P. vivax and P. malariae ranged from 62.30 to 63.15, 63.90 to 65.29, 66.67 to 68.40, 61.66 to 63.25 and 64.09 to 76.36 in case respectively. GC content of P. cynomolgi, P. ovale, P. falciparum, P. vivaxand P. malariae ranged from 36.85 to 37.70, 34.71 to 36.43, 31.60 to 33.27, 23.64 to 35...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28316416/murine-hepatocytes-dna-changes-as-an-assessment-of-the-immunogenicity-of-potential-anti-schistosomal-vaccines-experimentally
#16
Samia E Etewa, Mohamed H A Hegab, Ashraf S Metwally, Somia H Abd Allah, Sally M Shalaby, Amal S El-Shal, Mohamed Baredy, Mahmoud A El Shafey, Howayda S F Moawad
Hepatic affection by granulomatous inflammation in schistosomiasis suggested that a potential anti-pathology vaccine could be generated based on limiting the presence of hazardous hepatocytes induced apoptosis and caused reduction of granulomas number and size . So, this work is concerned with experimental assessment of the efficacy of different Schistosoma mansoni antigens (SEA, SWAP and combined SEA and SWAP) on murine liver after challenge by Schistosoma infection, histopathological, histochemical and molecular investigations were performed on sixty male laboratory bred Swiss Albino mice...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28316415/molecular-and-pathological-studies-on-natural-cases-of-bovine-theileriosis
#17
Anamika Gupta, Kuldip Gupta, Geeta Devi Leishangthem, Mandeep Singh Bal, N K Sood, Amarjit Singh
The present communication is a part of study conducted on 32 cases of bovine lymphadenopathies. Out of which, six cases of bovine theileriosis were diagnosed on the basis of peripheral blood smear examination and gross lesions in lymph nodes and visceral organs. Nested PCR using two primer sets N516/N517 and 14136/294 was conducted on samples collected from affected lymph nodes confirms Theileria annulata infection in five out of six cases. Sequencing analysis of amplified product showed 92, 94 and 93 % homology of isolate TH1_Bovine, TH2_Bovine and THEN_Bovine for T...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28316414/first-occurrence-of-argathona-macronema-bleeker-1857-isopoda-cirolanidae-on-coral-reef-fishes-along-the-tuticorin-coastal-water-gulf-of-mannar
#18
Gurusamy Chelladurai, Jayaraj Mohanraj, Govinda Rajalu Jayanthi
Isopod Parasites are considered as a major threat to the fishing industry, due to rigorous disease outbreaks. The parasites from the two economically important reef fish family Lutjanidae and Serranidae are analyzed i.e. Epinephelus malabaricus and Lutjanus rivulatus. The samples are obtained from the landings of Trawl net from Gulf of Mannar region of South east coast of India. They are severely infested by the isopod parasite Agrathona macronema in the Pectoral fins and ventral region of the body. This species is found to be the new record in the Gulf of Mannar region and deposited in the Marine laboratory of Kamaraj College...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28316413/detection-and-genotyping-of-toxoplasma-gondii-isolated-from-soil-in-ahvaz-southwest-of-iran
#19
J Saki, S Khademvatan, E Yousefi, M Tavalla, R Abdizadeh
To detection and genotype of Toxoplasma gondii isolated from soil in Ahvaz, southwest of Iran. Between August 2011 and May 2012 at different sites located in the area of the Ahvaz city south west Iran. A total of 200 soil samples were taken from different points of the region. Oocysts were recovered using the flotation method. Then, PCR reactions targeting the GRA6 gene were performed for specific T. gondii detection. The positive samples were studied by RFLP (random amplified fragment length polymorphism) using MseI enzymes to confirm the parasite linage...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28316412/larval-infestation-in-a-common-pariah-kite-milvus-migrans-boddaert
#20
Shuvra Kanti Sinha, Arup Kumar Sinha, Pradip De, Somnath Bhakat
This paper describes a case of larval infestation in a common Pariah Kite Milvus migrans (Boddaert). Examination on the larvae revealed that the larvae were of the family Sarcophagidae (Diptera).
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
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