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Apoptosis: An International Journal on Programmed Cell Death

Debashri Manna, Rajabrata Bhuyan, Forid Saikh, Somnath Ghosh, Jayasri Basak, Rita Ghosh
1,4-Dihydropyridines (1,4-DHPs) are important as a class of heterocyclic compounds that exhibit wide range of biological actions. Many of its derivatives are already characterized as medicinally important drugs and used worldwide. In this study, we have screened some novel Hantzsch 1,4-DHP compounds using both in silico (QSAR and Pharmacophore) and in vitro (cytotoxic screening). 1,4-DHP showed selective cytotoxicity against five human cancerous cell lines; A375, A549, HeLa, HepG2 and SH-SY5Y but limited effect towards normal skin keratinocyte (HaCaT), lung fibroblast (WL-38) and healthy peripheral blood mononuclear cells...
September 10, 2018: Apoptosis: An International Journal on Programmed Cell Death
Tanusree Ray, Dwiprohi Kar, Ananda Pal, Shravanti Mukherjee, Chandrima Das, Amit Pal
A novel activating peptide was designed and synthesized from V. cholerae hemagglutinine protease (HAP) mediated cleavage site of mouse PAR1. The peptide "PFISED" interacts with PAR1 in a new site which is different from its thrombin mediated conventional activation site and induced a series of new downstream signaling pathways. The peptide showed apoptosis in human and mouse breast (MCF-7 and EAC) and colon (HT29 and CT26) cancer cells where as in the same peptide concentration in normal human breast epithelial cells (MCF-10A), normal human fibroblast cells (MRC-5), normal mouse peritoneal macrophage cells and normal mouse breast and colon tissues did not show any effect...
September 8, 2018: Apoptosis: An International Journal on Programmed Cell Death
Matthew A Taylor, Bhaskar C Das, Swapan K Ray
Autophagy is an evolutionarily conserved catabolic process that plays an essential role in maintaining cellular homeostasis by degrading unneeded cell components. When exposed to hostile environments, such as hypoxia or nutrient starvation, cells hyperactivate autophagy in an effort to maintain their longevity. In densely packed solid tumors, such as glioblastoma, autophagy has been found to run rampant due to a lack of oxygen and nutrients. In recent years, targeting autophagy as a way to strengthen current glioblastoma treatment has shown promising results...
August 31, 2018: Apoptosis: An International Journal on Programmed Cell Death
Bin Chen, Xianbin Cao, Huayi Lu, Pengbo Wen, Xiaojing Qi, Shaopeng Chen, Lijun Wu, Chi Li, An Xu, Guoping Zhao
As a quorum-sensing molecule for bacteria-bacteria communication, N-(3-oxododecanoyl)-homoserine lactone (C12) has been found to possess pro-apoptotic activities in various cell culture models. However, the detailed mechanism of how this important signaling molecule function in the cells of live animals still remains largely unclear. In this study, we systematically investigated the mechanism for C12-mediated apoptosis and studied its anti-tumor effect in Caenorhabditis elegans (C. elegans). Our data demonstrated that C12 increased C...
August 31, 2018: Apoptosis: An International Journal on Programmed Cell Death
Chunli Chi, Liguo Wang, Wenwen Lan, Long Zhao, Ying Su
Apoptosis is one of the main fundamental biological processes required for development of multicellular organisms. Inappropriate regulation of apoptosis can lead to severe developmental abnormalities and diseases. Therefore, the control of apoptosis, not only for its activation but also for its inhibition, is critically important during development. In contrast to the extensive studies of apoptosis induction, its inhibitory mechanisms that are even more vital in certain populations of cells actually are very far from being well understood...
August 29, 2018: Apoptosis: An International Journal on Programmed Cell Death
Jie Gao, Ruina Kong, Xiaoli Zhou, Lianmei Ji, Ju Zhang, Dongbao Zhao
Both miR-126 and IL-23R affect rheumatoid arthritis (RA) procession. This study aimed to investigate the association of miR-126 and IL-23R and the possible modulation of miR-126 to RA pathogenesis. Serum, synovial tissue and synovial fluid were collected from patients with RA, and expression of miR-126, IL-23R, TNF-α and IFN-γ were detected. Fibroblast-like synoviocytes (FLS) was established using a collagen-induced arthritis mice model. The expression of miR-126 was manual intervened using pro-miR-126 and anti-miR-126 encoding lentivirus plasmids, or miR-126 agonists and corresponding negative controls...
August 24, 2018: Apoptosis: An International Journal on Programmed Cell Death
Rui Chen, Meiping Jiang, Bo Li, Wei Zhong, Zhongqun Wang, Wei Yuan, Jinchuan Yan
Autophagy is a recycling process that degrades damaged or unneeded cellular components for renewal. Accumulating evidence suggests that dysregulation of autophagy is involved in pulmonary hypertension (PH). PH is a progressive disease characterized by persistent proliferation of apoptosis-resistant pulmonary vascular cells. However, reports on the role of autophagy in the development of PH are often conflicting. In this review, we discuss recent development in the field with emphasis on pulmonary arterial endothelial cells, pulmonary smooth muscle cells, right ventricular myocyte, as well as pharmacological strategies targeting the autophagic signaling pathway...
August 16, 2018: Apoptosis: An International Journal on Programmed Cell Death
Mansoureh Hashemi, Alireza Zali, Javad Hashemi, Saeed Oraee-Yazdani, Akhtar Akbari
Strong 14-3-3 zeta protein expression plays an important role in tumorigenesis, including in the maintenance of cell growth, resistance increase, and the prevention of apoptosis. In this study, we focus on two targets: (1) the expression of 14-3-3 zeta in the different grades of human astrocytoma (II-IV), (2) suppression of 14-3-3 zeta protein expression in glioblastoma derived astrocytes by 14-3-3 zeta shRNA lentiviral particles. The tissues of human astrocytoma were provided from 30 patients (ten of each grade of astrocytoma)...
August 12, 2018: Apoptosis: An International Journal on Programmed Cell Death
Wen-Xiang Cao, Ting Li, Zheng-Hai Tang, Le-Le Zhang, Zhao-Yu Wang, Xia Guo, Min-Xia Su, Xiuping Chen, Jin-Jian Lu
The pseudokinase mixed lineage kinase domain-like protein (MLKL) is a core effector of necroptosis, and its function in necroptosis is widely studied. However, the function of MLKL in apoptosis remains unclear. In the present study, the role of MLKL in chelerythrine (CHE)-promoted apoptosis was studied. A special band of MLKL (i.e., *MLKL) was observed after treatment with CHE. MLKL and *MLKL were accumulated in the nucleus upon treatment with CHE and MLKL silencing reversed the CHE-induced apoptosis. Blockade of CHE-triggered reactive oxygen species (ROS) generation or inhibition of CHE-activated protein kinase-like endoplasmic reticulum kinase (PERK)-eukaryotic initiation factor 2 α subunit (eIF2α) pathway reversed the apoptosis...
August 6, 2018: Apoptosis: An International Journal on Programmed Cell Death
Jian Gao, Qianqian Zheng, Yue Shao, Wei Wang, Chenghai Zhao
CD155 has been implicated in migration, invasion, proliferation and apoptosis of human cancer cells, and DNA damage response caused by chemotherapeutic agents or reactive oxygen species has been shown to attribute to CD155 induction. Adriamycin (Adr) is one of the most common chemotherapeutic drugs used to treat breast cancer. Here we reported that treatment with Adr upregulated CD155 expression on several in vitro cultured breast cancer cells and in breast cancer cell 4T1 xenografts. We also found that CD155 knockdown or Adr treatment induced apoptosis of in vitro cultured cancer cells and cancer cells in 4T1 xenografts, and a combination of CD155 knockdown with Adr treatment induced more cell death than either of them...
July 23, 2018: Apoptosis: An International Journal on Programmed Cell Death
Małgorzata Durbas, Paweł Pabisz, Katarzyna Wawak, Aneta Wiśniewska, Elżbieta Boratyn, Iwona Nowak, Irena Horwacik, Olga Woźnicka, Hanna Rokita
The process of autophagy and its role in survival of human neuroblastoma cell cultures was studied upon addition of an anti-GD2 ganglioside (GD2) 14G2a mouse monoclonal antibody (14G2a mAb) and an aurora A kinase specific inhibitor, MK-5108. It was recently shown that combination of these agents significantly potentiates cytotoxicity against IMR-32 and CHP-134 neuroblastoma cells in vitro, as compared to the inhibitor used alone. In this study we gained mechanistic insights on autophagy in the observed cytotoxic effects exerted by both agents using cytotoxicity assays, RT-qPCR, immunoblotting, and autophagy detection methods...
July 19, 2018: Apoptosis: An International Journal on Programmed Cell Death
Anna Kozłowska, Piotr Kozera, Mariusz Majewski, Janusz Godlewski
Neoplastic process may cause distinct changes in the morphology, i.e. size and number of the neurons of the neuronal plexuses forming the enteric nervous system (ENS) of the human intestine. Moreover, it was also reported that these changes were not directly associated with apoptosis. Thus, the main aim of this study was to determine the atrophic changes of myenteric plexuses (MPs) in the vicinity of cancer invasion and the potential reason which may be responsible for these changes if they occur. Tissue samples from the stomach were collected from ten patients which undergo organ resection due to cancer diagnosis...
July 17, 2018: Apoptosis: An International Journal on Programmed Cell Death
Sai-Juan Xu, Fan Zhang, Li-Juan Wang, Ming-Hua Hao, Xian-Jun Yang, Na-Na Li, Hong-Long Ji, Ping Xu
The present study evaluated the protective effect of the natural compound flavonoids of Rosa roxburghii Tratt (FRT) against γ-radiation-induced apoptosis and inflammation in mouse thymus cells in vivo and in vitro. Thymus cells and mice were exposed to 60 Co γ-ray at a dose of 6 Gy. The radiation treatment induced significant cell apoptosis and inflammation. Radiation increased the expressions of cleaved caspase 3/8-10, AIF, and PARP-1, and FRT could mitigate their activation and inhibit subsequent apoptosis in the thymus both in vitro or in vivo...
July 11, 2018: Apoptosis: An International Journal on Programmed Cell Death
Hui Ma, Shaoyu Liu, Ying Xiong, Zhanwen Zhang, Aixia Sun, Shu Su, Hong Liang, Gongjun Yuan, Ganghua Tang
Cardiomyocyte apoptosis has been observed in several cardiovascular diseases and contributes to the subsequent cardiac remodeling processes and progression to heart failure. Consequently, apoptosis imaging is helpful for noninvasively detecting the disease progression and providing treatment guidance. Here, we tested 18 F-labeled 2-(5-fluoropentyl)-2-methyl-malonic acid (18 F-ML-10) and 18 F-labeled 2-(3-fluoropropyl)-2-methyl-malonic acid (18 F-ML-8) for apoptosis imaging in rat models of myocardial infarction (MI) and compared them with 18 F-fluorodeoxyglucose (18 F-FDG)...
June 23, 2018: Apoptosis: An International Journal on Programmed Cell Death
Miao Liu, Yilu Wang, Qian Zhu, Junyu Zhao, Yao Wang, Man Shang, Minglin Liu, Yanna Wu, Junqiu Song, Yanxia Liu
Microvesicles (MVs) have been shown to be involved in pathophysiology of ischemic heart diseases. However, the underlying mechanisms are still unclear. Here we investigated the effects of MVs derived from ischemic preconditioning (IPC-MVs) on myocardial ischemic/reperfusion (I/R) injury in rats. Myocardial IPC model was elicited by three cycles of ischemia and reperfusion of the left anterior descending (LAD) coronary artery. IPC-MVs from the peripheral blood of the above animal model were isolated by ultracentrifugation and characterized by flow cytometry and transmission electron microscopy...
August 2018: Apoptosis: An International Journal on Programmed Cell Death
Michael A Model, Nathan J Mudrak, Priyanka S Rana, Robert J Clements
Apoptotic volume decrease (AVD) is a characteristic cell shrinkage observed during apoptosis. There are at least two known processes that may result in the AVD: exit of intracellular water and splitting of cells into smaller fragments. Although AVD has traditionally been attributed to water loss, direct evidence for that is often lacking. In this study, we quantified intracellular water in staurosporine-treated cells using a previously described optical microscopic technique that combines volume measurements with quantitative phase analysis...
August 2018: Apoptosis: An International Journal on Programmed Cell Death
Mustapha Kandouz
The original version of this article contained a mistake in reference. The references in Table 1 are incorrect. The corrected Table with proper citation is given below. The field codes ADDIN REFMGR.CITE inadvertently appeared along the article. This was overlooked during the process.
August 2018: Apoptosis: An International Journal on Programmed Cell Death
Bhanu Priya Awasthi, Kalyan Mitra
Natamycin, a Food and Drug Administration approved anti-fungal drug, and also used as a food additive was evaluated for anti-leishmanial activity since it is known to specifically bind to ergosterol, which is essential to these parasites but absent in mammals. Promising anti-proliferative activity was observed in both promastigote and amastigote forms of the parasite with IC50 values of 15 and 8 µM respectively and a selective index of 12.5. The ultrastructural effects of natamycin on both forms of the parasite and physiological effects on promastigotes were studied in detail for the first time...
August 2018: Apoptosis: An International Journal on Programmed Cell Death
Zhongyan Hua, Yue Zhan, Simeng Zhang, Yudi Dong, Min Jiang, Fei Tan, Zhihui Liu, Carol J Thiele, Zhijie Li
The over-expressions of brain-derived neurotrophic factor (BDNF) and its tyrosine kinase receptor TrkB have been reported to induce chemo-resistance in neuroblastoma (NB) cells. In this study, we investigated the roles of P53 and BCL2 family members in the protection of BDNF/TrkB from etoposide-induced NB cell death. TB3 and TB8, two tetracycline (TET)-regulated TrkB-expressing NB cell lines, were utilized. The expressions of P53 and BCL2 family members were detected by Western blot or RT-PCR. Transfection of siRNAs was used to knockdown P53 or PUMA...
August 2018: Apoptosis: An International Journal on Programmed Cell Death
Alexandre Iscaife, Sabrina Thalita Reis, Denis Reis Morais, Nayara Izabel Viana, Iran Amorim da Silva, Ruan Pimenta, Andre Bordini, Nelson Dip, Miguel Srougi, Katia Ramos Moreira Leite
Prostate cancer (PCa) is an incurable disease at the metastatic stage. Although there are different options for treatment, the results are limited. MicroRNAs (miRNAs) are a group of small, noncoding, regulatory RNAs with important roles in regulating gene expression. miR-145 is reported to be a key tumor suppressor miRNA (tsmiR) that controls important oncogenes, such as MYC and RAS. In this study, in vitro studies were performed to show the control of MYC and RAS by miR-145. Flow cytometry was used to analyze cell proliferation and apoptosis...
August 2018: Apoptosis: An International Journal on Programmed Cell Death
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