journal
https://read.qxmd.com/read/35753793/low-temperature-heating-induced-death-and-vacuole-injury-in-cladosporium-sphaerospermum-conidia
#21
JOURNAL ARTICLE
Shigetoshi Horikiri, Mami Harada, Ryoko Asada, Jin J Sakamoto, Masakazu Furuta, Tetsuaki Tsuchido
The mechanism of thermal death of mold conidia has not been understood in detail. The purpose of this study is to analyze the death kinetics of heated conidia of Cladosporium sphaerospermum and to ascertain the expectant cell injury responsible for the death. The death of the dormant (resting) conidia of Cladosporium sphaerospermum was examined at temperatures of between 43 and 54℃ with the conventional colony count method. The death reaction apparently followed the first order kinetics, but the Arrhenius plot of the death rate constant demonstrated seemingly a break...
2022: Biocontrol Science
https://read.qxmd.com/read/35314562/effects-of-violet-blue-light-emitting-diode-on-controlling-bacterial-contamination-in-boiled-young-sardine
#22
JOURNAL ARTICLE
Akihiro Shirai, Yu-Ko Yasutomo, Yuka Kanno
The aim of this study was to evaluate bacterial decontamination of boiled young sardine by treatment with violet-blue light followed by cooling storage of the irradiated boiled sardine. Viable cell count in the samples was evaluated after irradiation with four types of violet-blue light-emitting diodes (LEDs; peak wavelength at 405, 412, 421 or 455 nm) and subsequent cooling storage for two days. LED (405 nm) exhibited bactericidal and growth suppression effects. The irradiation gave a 47% bactericidal rate in comparison with no irradiation samples (control) and the two-day storage suppressed the increase in cell counts to 24%, while the rate of increase was 545% for the control...
2022: Biocontrol Science
https://read.qxmd.com/read/35314561/shellcoat-a-calcinated-calcium-solution-effectively-inactivates-sars-cov-2
#23
JOURNAL ARTICLE
Noritoshi Hatanaka, Bingting Xu, Yasuharu Yamashita, Hiroo Kawakami, Mayo Yasugi, Shinji Yamasaki
SARS-CoV-2, an acute respiratory syndrome-causing virus, suddenly emerged at the end of 2019 in China, and rapidly spread all over the world. In this study, we examined whether a calcinated calcium solution (ShellCoat) , which has been approved as a food additive in Japan can inactivate SARS-CoV-2. Furthermore, antiviral activity of ShellCoat against SARS-CoV-2 was also evaluated in the presence of organic matter, namely, fetal bovine serum (FBS) . When concentrated SARS-CoV-2 were treated with ShellCoat for 10 sec in presence or absence of FBS as organic matters, the viral titer was decreased more than 4 logs 50% tissue culture infective dose per mL (TCID50 /mL) but use of ShellCoat for 20 sec or more under similar experimental conditions the viral titer was below the detection limit (≦2...
2022: Biocontrol Science
https://read.qxmd.com/read/35314560/formation-of-a-specific-dmpo-oh-adduct-signal-related-to-hydroxy-radical-generation-in-a-long-term-stored-improved-iodide-formulation
#24
JOURNAL ARTICLE
Nori Kida
A specific DMPO-OH adduct signal (1:2:2:1)related to hydroxyl radical generation in a longterm stored improved iodide formulation, tentatively designated as the distilled KMT reagent which prepared from a pH 0.3 solution containing FeCl3 , EDTA, KI and ethanol termed the KMT reagent, was detected by electron spin resonance (ESR) spectroscopy.Although the color intensities of N,N,N,N-tetramethyl-p-phenylenediamine (TMPD) and N,N-diethyl-p-phenylenediamine (DPD) differ, the mixture of the long-term stored distilled KMT reagent and TMPD exhibited a purple color similar to Wurster's blue, and the mixture of the long-term stored distilled KMT reagent and DPD exhibited a pink color similar to Wurster's red...
2022: Biocontrol Science
https://read.qxmd.com/read/35314559/sterilizing-ability-of-high-voltage-pulsed-discharge-plasma-with-cavitation-for-microorganisms-including-radio-resistant-bacterium-in-water
#25
JOURNAL ARTICLE
Hiroaki Terato, Yuka Tokuyama, Hiroki Nishiyama, Takashi Matsunaga, Yuki Yoshida, Satoshi Ihara
There are various purification methods have been developed and applied to industrial wastewater with contaminated microorganisms. We previously reported that high-voltage pulsed discharge plasma with cavitation effectively kills Escherichia coli cells. We attempted to expand the application of this disinfection method by using microorganisms such as Bacillus subtilis, Deinococcus radiodurans, and Schizosaccharomyces pombe. These microbial cells were treated with the discharge plasma, and the cell viability, DNA damage, and morphological changes were analyzed to evaluate the bactericidal effect...
2022: Biocontrol Science
https://read.qxmd.com/read/35314558/ellagic-acid-combined-with-tacrolimus-showed-synergistic-cell-growth-inhibition-in-fission-yeast
#26
JOURNAL ARTICLE
Kanako Hagihara, Kousuke Hosonaka, Shuhei Hoshino, Kazuki Iwata, Naoki Ogawa, Ryosuke Satoh, Teruaki Takasaki, Takuya Maeda, Reiko Sugiura
Calcineurin (CN) is a conserved Ca2+ -calmodulin activated protein phosphatase, which plays important roles in immune regulation, cardiac hypertrophy, and apoptosis in humans. In pathogenic fungi, CN is essential for stress survival, sexual development, and virulence. The immunosuppressant tacrolimus (FK506) is a specific inhibitor of CN in humans and fungi including nonpathogenic fission yeast. Although calcineurin inhibition by FK506 or CN deletion in fission yeast does not induce growth defects, treatment with some anti-fungal drugs such as micafungin and valproic acid, induced synthetic lethality with calcineurin inhibition...
2022: Biocontrol Science
https://read.qxmd.com/read/35314557/effects-of-oakmoss-components-on-extra-and-intracellular-legionella-pneumophila-and-its-host-acanthamoeba-castellanii
#27
JOURNAL ARTICLE
Harue Nomura, Nobumasa Suda, Yasuyo Kawano, Yasunori Isshiki, Keisuke Sakuda, Katsuya Sakuma, Seiichi Kondo
Acanthamoeba castellanii is a ubiquitous organism found in environmental water. The amoeba is pathogenic to toward humans and is also a reservoir of bacteria of the genus Legionella, a causative agent of legionellosis. Oakmoss, a source of natural fragrance ingredients, and its components are antibacterial agents that are specifically active against the genus Legionella. In the present study, oakmoss and its components were investigated for their inhibitory effects on total (extra- and intracellular) Legionella pneumophila within A...
2022: Biocontrol Science
https://read.qxmd.com/read/35314556/ultraviolet-light-emitting-diode-uv-led-sterilization-of-citrus-bacterial-canker-disease-targeted-for-effective-decontamination-of-citrus-sudachi-fruit
#28
JOURNAL ARTICLE
Akihiro Suzuki, Akira Emoto, Akihiro Shirai, Kentaro Nagamatsu
A kind of citrus fruit with special flavor, Citrus sudachi harvested in Japan, are exported to various countries. However, the Citrus sudachi needs to be sterilized using aqueous solution of sodium hypochlorite because there is a possibility of the adhesion of citrus bacterial canker (CBC) which is not found in Europe. Due to the sterilization with time-consuming work, a more effective decontamination technique is required. A decontamination method using ultraviolet (UV) light irradiation is thus anticipated...
2022: Biocontrol Science
https://read.qxmd.com/read/35034931/two-potential-probiotic-bacillus-with-proteolytic-activity-to-dietary-protein-from-adult-feces
#29
JOURNAL ARTICLE
Haiqi Wang, Xichun Zhang, Zhaohua Chen, Gengxin Hao, Guiling Li
 Two Bacillus strains were screened and identified using 16S rRNA gene sequencing the phenotypic tests, and then characterized in vitro for the probiotic characteristics. They were able to tolerate pH 2.5 for 2.5 h, following 0.3% bile salts and 0.1% pancreatin treatment for 5 h. They exhibited good ability to attach to intestinal epithelial cells and were susceptible to most of the antibiotics and being killed by several. Further and more important, they showed good proteolytic activity to food protein as gelatin and milk, with even higher activity than the reference strain...
2021: Biocontrol Science
https://read.qxmd.com/read/35013019/comparison-of-benzo%C3%AF-a%C3%AF-pyrene-degrading-activities-between-olleya-sp-itb9-isolated-from-tokyo-bay-and-other-type-strains-of-the-genus-olleya
#30
JOURNAL ARTICLE
Naoto Urano, Aya Matsushima, Shione Yamamoto, Rintaro Endo, Masami Ishida, Masahiko Okai, Toshinori Takei, Masachika Takashio
Benzo[a]pyrene (BaP) is one of the strongest carcinogenic compounds among polycyclicaromatic hydrocarbons (PAHs) .We previously identified the ITB9 strain of Olleya species, which shows BaP-degrading activity; our report was the first about BaP degradation by the genus Olleya. In this study, BaP-degradation efficiency by ITB9 was about 50% when the strain was suspended in 20 ml of L9 liquid medium with 100 μg/ml BaP and 0.2 M NaCl, with pH 8.0, and incubated at 25℃ for 5 days. Under the same conditions, all four type strains (O...
2021: Biocontrol Science
https://read.qxmd.com/read/35013018/detection-time-distribution-of-microcolonies-formed-by-individual-heat-injured-cells-of-escherichia-coli
#31
JOURNAL ARTICLE
C Vo Khanh, Enami Tomii, Ryoko Asada, Jin J Sakamoto, Masakazu Furuta, Tetsuaki Tsuchido
The microcolony formation at 30℃ on an enriched minimal salts agar plates by individual Escherichia coli cells heated at 50℃ was monitored with a time-lapse shadow image analysis system, MicroBio μ3DTM AutoScanner. While the time course of microcolony count detected every half an hour for the unheated cells seemingly demonstrated a normal distribution, that for the heated cell population demonstrated totally the growth delay probably resulting from cell injury and also interestingly distributed in its rather deformed pattern with a tailing...
2021: Biocontrol Science
https://read.qxmd.com/read/35013017/influenza-outbreak-and-a-group-meal-in-a-geriatric-long-term-care-facility-in-japan
#32
JOURNAL ARTICLE
Akio Yoshimura, Teppei Sasahara, Ryusuke Ae, Koki Kosami, Dai Akine, Masanori Ogawa, Kenji Hamabata, Shuji Hatakeyama, Yuji Morisawa, Longzhu Cui
Influenza outbreaks at geriatric long-term care facilities (g-LTCFs) can be deadly and their prevention is important. However, the factors influencing disease transmission in g-LTCFs remain controversial. In this descriptive study, we tried to identify the potential risk factors influencing influenza outbreaks that occurred in different influenza seasons within a single g-LTCF with 100 residents in Gunma Prefecture. We reviewed the detailed facility records for all influenza cases in both residents and staff between January 2012 and June 2020...
2021: Biocontrol Science
https://read.qxmd.com/read/35013016/antifungal-prospect-of-bacillus-cereus-postbiotic-on-crustacean-pathogen-lagenidium-thermophilum
#33
JOURNAL ARTICLE
Joning Elliecpearl Jasca, Kian Yong Annita Seok, Lajimin Suraini, Yao Ang Chun, Ransangan Julian, Motohiko Sano, Shigeki Dan, Katsuyuki Hamasaki, Kasan Nor Azman, M Lal Tamrin
Pathogenic marine fungi, Lagenidium thermophilum is known causative agent in the crustacean industry. Current disinfection practice in hatchery has risks and negative impacts which prompts suitable substitute to synthetic antifungal agents. Thus, this study was conducted to evaluate the antifungal potential of postbiotic from four potential probiotics towards marine oomycetes, L. thermophilum IPMB 1401. The screening test showed that the Lactobacillus plantarum GS12 and Bacillus cereus GS15 postbiotics were positive for antifungal activity on L...
2021: Biocontrol Science
https://read.qxmd.com/read/35013015/effect-of-the-combination-of-high-hydrostatic-pressure-and-alkaline-electrolyzed-water-on-the-reduction-of-heat-resistance-of-bacterial-spores
#34
JOURNAL ARTICLE
Daisuke Hamanaka, Masaki Naka, Kyohei Arimura
The effect of combined use of alkaline electrolyzed water (AlEW) on the reduction of heat resistance of bacterial spores by high hydrostatic pressure processing( HPP) was investigated in this study. No reduction of heat resistance of bacterial spores, which was defined as the spore survival by heat treatment at 80℃ for 15 min, was observed by the treatment of single HPP with 30MPa at 50℃ even for 6 hours. However, a 3-log decrease in the viable bacterial spores was obtained by the combination of AlEW pretreatment with 1 hour of HPP treatment...
2021: Biocontrol Science
https://read.qxmd.com/read/35013014/microbial-contamination-of-rubber-ducks-floating-in-bathtubs-of-bathing-facilities-and-an-evaluation-of-their-washing-methods
#35
JOURNAL ARTICLE
Akiko Edagawa, Norihiro Matsuda, Toru Ogura, Kenichi Uezono, Shinji Izumiyama, Akira Fujii
Microbiological contamination inside rubber ducks floating in the bathtub at a "duck bath" of a bathing facility was analyzed by examining bacterial and amoebic counts. The results of microbial tests, such as standard plate count, heterotrophic plate count and Legionella-LAMP (Loopmediated isothermal amplification) , showed that microbial contamination increased in the rubber ducks over time. When the ducks were washed with sodium hypochlorite, those microbial contaminations were not detected; but when the ducks were washed with an electrolyzed water, the standard plate counts and the heterotrophic plate counts were detected in the amount of 103 per duck in the wipe samples...
2021: Biocontrol Science
https://read.qxmd.com/read/34556621/molecular-characterization-virulence-and-antimicrobial-susceptibility-testing-of-riemerella-anatipestifer-isolated-from-ducklings
#36
JOURNAL ARTICLE
Amany Shousha, Amal Awad, Gamal Younis
This pilot study aimed to characterize Riemerella anatipestifer from ducklings, testing their susceptibility to antimicrobial agents and to detect their virulence markers. Seven R. anatipestifer isolates with 11.67% infection rate were identified out of sixty freshly dead ducklings and confirmed by PCR assay targeting gyrB gene. The gyrB gene sequences of R. anatipestifer isolates were 100% identical to each other and also showed 100% sequence similarity to the published gyrB genes. Four virulence genes namely ompA, prtC, hagA, and sspA were identified in all isolates except sspA was detected in 5 isolates...
2021: Biocontrol Science
https://read.qxmd.com/read/34556620/ethanol-susceptibility-of-sars-cov-2-and-other-enveloped-viruses
#37
JOURNAL ARTICLE
Toshihito Nomura, Tanuza Nazmul, Reiko Yoshimoto, Akifumi Higashiura, Kosuke Oda, Takemasa Sakaguchi
Ethanol is an effective disinfectant against the novel coronavirus SARS-CoV-2. However, its effective concentration has not been shown, and we therefore analyzed the effects of different concentrations of ethanol on SARS-CoV-2. When SARS-CoV-2 was treated with varying ethanol concentrations and examined for changes in infectivity, the ethanol concentration at which 99% of the infectious titers were reduced was 24.1% (w/w) [29.3% (v/v)]. For reference, ethanol susceptibility was also examined with other envelope viruses, including influenza virus, vesicular stomatitis virus in the family Rhabdoviridae, and Newcastle disease virus in the family Paramyxoviridae, and the 99% inhibitory concentrations were found to be 28...
2021: Biocontrol Science
https://read.qxmd.com/read/34556619/virulent-and-multiple-antimicrobial-resistance-aeromonas-hydrophila-isolated-from-diseased-nile-tilapia-fish-oreochromis-niloticus-in-egypt-with-sequencing-of-some-virulence-associated-genes
#38
JOURNAL ARTICLE
Amany Saleh, Rasha Elkenany, Gamal Younis
Aeromonas hydrophila is a major waterborne pathogen, which induces various diseases in freshwater fish with the capability for zoonotic potential. This study was applied to investigate the prevalence of A. hydrophila in diseased Nile tilapia fish, genetic characterization of the virulence encoding genes (act, aerA, alt, and ast genes), and antibiotic susceptibility. Out of the 500 diseased Nile tilapia fish samples, 70% (350/500) Aeromonas species were isolated. From which 53.4% (187/350) of Aeromonas hydrophila strains were identified...
2021: Biocontrol Science
https://read.qxmd.com/read/34556618/isolation-and-characterization-of-the-polyhexamethylene-biguanide-hydrochloride-resistant-fungus-purpureocillium-lilacinum
#39
JOURNAL ARTICLE
Takako Yamamoto, Yikelamu Alimu, Hiroki Takahashi, Yoko Kusuya, Kouichi Hosoya, Naofumi Shigemune, Satoshi Nagai, Takashi Yaguchi
We isolated a fungus from a 20% (= 200,000 µg/mL) aqueous solution of polyhexamethylene biguanide hydrochloride (PHMB), a widely used antimicrobial and examined its morphology and drug resistance profile. Based on the sequence of the internal transcribed spacer region of ribosomal DNA, the fungus was identified as Purpureocillium lilacinum. Although the P. lilacinum type and resistant strains showed similar morphology, the latter had extremely low PHMB susceptibility and was able to grow in 20% aqueous solution of PHMB, which eliminated the type strain...
2021: Biocontrol Science
https://read.qxmd.com/read/34556617/spore-heat-resistance-and-growth-ability-at-refrigeration-temperatures-of-bacillus-spp-and-paenibacillus-spp
#40
JOURNAL ARTICLE
Tetsuya Kobayashi, Takanori Azuma, Daisuke Yasokawa, Shogo Yamaki, Koji Yamazaki
In this study, spore heat resistance and growth ability at refrigeration temperatures of Bacillus spp. and Paenibacillus spp. were determined. The spore D90°C of 67.6% (23 of 34 strains) of Bacillus and 73.9% (17 of 23 strains) of Paenibacillus was less than 15 min. The growth abilities of both genera were equivalent at 10°C. However, 71.1% (32 of 45 strains) of Paenibacillus and only 6.3% (3 of 48 strains) of Bacillus cereus group could grow at 4°C. Eight B. cereus strains formed spores with higher heat resistance compared to the other Bacillus strains assessed; however, they did not grow at tempreratures below 10°C...
2021: Biocontrol Science
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