journal
https://read.qxmd.com/read/36567120/use-of-atp-bioluminescence-assay-to-evaluate-oral-streptococci
#1
JOURNAL ARTICLE
Akari Watanabe, Naofumi Tamaki, Kenji Yokota, Susumu Kokeguchi, Hiro-O Ito, Miwa Matsuyama
The statistical correlation between the number of oral streptococci and the results of ATP bioluminescence assay was examined and compared with the results from Streptococcus plate counts and an oral bacteria quantification system. Because a significant correlation was found between ATP (RLU) and the number of bacteria in the oral bacteria quantification system for all seven types of oral streptococci examined, ATP would reflect a conditions of oral hygiene. However, using this assay, it was observed it may be difficult to correctly evaluate bacteria that form aggregates...
2022: Biocontrol Science
https://read.qxmd.com/read/36567119/inactivation-of-sars-cov-2-by-commercially-available-disinfectants-and-cleaners
#2
JOURNAL ARTICLE
Y U Harada, Hitomi Amo, Takuto Ono, Hiroki Murai, Tadahiro Sasaki, Tatsuo Shioda, Masahito Ikawa, Yoshihiko Hirata
The recent emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a major burden for health care systems worldwide, and is a threat to our daily lives. Various effective ingredients against SARS-CoV-2 were already reported, however, since products contain various ingredients, it is also important to evaluate the effectiveness of commercially available disinfectants per se. In this study, the virucidal efficacy of forty-eight commercially available products were evaluated according to the standardized suspension method EN 14476 and the following results were obtained: Alcohol-based disinfectants, hand soaps, wet wipes, alkaline cleaners, quaternary ammonium compound sanitizers and oxygen bleach had great virucidal efficacy against SARS-CoV-2...
2022: Biocontrol Science
https://read.qxmd.com/read/36567118/remote-bactericidal-effect-of-anatase-tio-2-photocatalytic-nanoparticles-annealed-with-low-temperature-o-2-plasma
#3
JOURNAL ARTICLE
Retsuo Kawakami, Yuki Takao, Akihiro Shirai, Takashi Mukai
The remote bactericidal effect of TiO2 photocatalyst, i.e., the bactericidal effect away from the photocatalyst, was successfully achieved using a humidified airflow. The TiO2 photocatalyst used was anatase-type TiO2 nanoparticles (NPs) annealed with a low-temperature O2 plasma. For comparison, anatase-type TiO2 NPs annealed in the air were used. The bacteria, Bacillus subtilis, were placed away from the TiO2 NPs. The plasma-assisted-annealed TiO2 NPs significantly inactivated 99% of the bacterial cells in 5 h, whereas the pristine and air-annealed TiO2 NPs inactivated 88-90% of the bacterial cells...
2022: Biocontrol Science
https://read.qxmd.com/read/36567117/simple-and-rapid-detection-of-esbl-bla-shv-gene-from-an-urban-river-in-tokyo-by-loop-mediated-isothermal-amplification
#4
JOURNAL ARTICLE
Masahiko Okai, Rintaro Endo, Masachika Takashio, Masami Ishida, Naoto Urano
Extended-spectrum β-lactamases (ESBLs) are produced mainly by gram-negative bacteria in Enterobacteriaceae. One of the major types of ESBLs is sulfhydryl variable (SHV) -type ESBL. Herein, we attempted to develop a simple and rapid method for the detection of the ESBL blaSHV gene by loop-mediated isothermal amplification (LAMP) . The five-primer set designed could amplify blaSHV gene at an isothermal temperature of 65℃. The detection limit of the LAMP method with the LF loop primer was 1 copy/tube, which was 10,000-fold more sensitive than that of the conventional PCR...
2022: Biocontrol Science
https://read.qxmd.com/read/36567116/isolation-screening-and-identification-of-native-and-new-bacillus-subtilis-with-strong-antifungal-compound-against-fusarium-oxysporum
#5
JOURNAL ARTICLE
Fatemeh Rafiee, Mohammad Reza Fazeli, Abbas Akhavan Sepahi, Zahra Noormohammadi
The genus Fusarium causes a wide range of infections in human, animals and herbs. The purpose of this research was to investigate and identify the native strains of Bacillus subtilis playing an inhibitory role against Fusarium oxysporum by producing surfactin. B. subtilis was isolated from the soil of various parks in Tehran-Iran, and identified by biochemical tests. Growth inhibition zone, minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of B. subtilis were determined. After purification of surfactin, quantitative and qualitative analysis of surfactin conducted using high performance liquid chromatography (HPLC) ...
2022: Biocontrol Science
https://read.qxmd.com/read/36567115/the-effectiveness-of-neutral-electrolyzed-water-for-decontaminating-the-spray-nozzles-of-electric-tankless-and-tank-type-warm-water-bidet-toilet-seats
#6
JOURNAL ARTICLE
Toru Iyo, Keisuke Iwahori
Electrolyzed water is a novel disinfectant that is widely used in the food industry. We conducted an experimental model-based study to determine the effectiveness of neutral electrolyzed water (NEW) for the daily nozzle cleaning of artificially contaminated tankless and tank-type bidet toilet seats. The toilet seats were designed to automatically self-clean the spray nozzles using tap water or NEW after each use or at specified intervals. The numbers of Pseudomonas aeruginosa and Escherichia coli microorganisms in the spray water were measured twice or thrice per week...
2022: Biocontrol Science
https://read.qxmd.com/read/36567114/current-status-and-future-prospects-of-cladosporium-sp-a-biocontrol-agent-for-sustainable-plant-protection
#7
REVIEW
Md Touhidul Islam
The fungal biocontrol agents, Cladosporium sp. have great economic importance on account of their beneficial effects on the integrated pest management (IPM) program. The different species of this genus can control different arthropod pests like sweetpotato whitefly, sugarcane woolly aphid, two-spotted mite, cotton aphid, cotton leaf worm, red spider mite, armyworm; and different plant diseases like- rice blast, wheat stripe rust, chrysanthemum white rust, etc. Chemical pesticides are a common practice by the user to protect their crops from these pests...
2022: Biocontrol Science
https://read.qxmd.com/read/36216570/examination-of-conditions-for-external-quality-control-in-identification-of-microorganisms-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#8
JOURNAL ARTICLE
Kazuyuki Sogawa, Kohei Uechi, Makiko Kiyosuke, Syota Murata, Koji Kusaba, Yuji Saeki, Kazuki Horiuchi, Kazunari Yasuda, Hajime Okumura, Azumi Fujinaga, Masami Murakami
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was approved for medical use in 2011 and is currently used as a rapid, accurate and lowcost technique for bacterial identification. External quality control for medical analysis is monitored using tests of the Japanese Association of Medical Technologists and Prefectural Association of Clinical Laboratory Technologists and through user surveys of reagent and equipment manufacturers. However, external quality control of bacterial typing using MS is not performed...
2022: Biocontrol Science
https://read.qxmd.com/read/36216569/theoretical-base-for-the-application-of-the-divsal-method-to-bacterial-spores-to-evaluate-injured-populations-occurring-after-exposure-to-lethal-stress
#9
JOURNAL ARTICLE
Ryoko Asada, Jin J Sakamoto, Masakazu Furuta, Tetsuaki Tsuchido
To assess injury in bacterial spore populations exposed to lethal stress, we proposed a theoretical basis for applying the DiVSaL method, which has already been reported for general microorganisms as a double subculture method. We constructed a mathematical model in which both injuries to the germination system and the spore body were taken into the theory. In this theory, we reasonably assumed that the viable and germinable spore count is constant before the subsequent vegetative growth and that the delay of germination and outgrowth can be included in the concept of λ injury previously reported as the growth-independent injury...
2022: Biocontrol Science
https://read.qxmd.com/read/36216568/effect-of-low-temperature-stock-on-the-growth-of-bacteria
#10
JOURNAL ARTICLE
Mitsuyo Kita, Jun Usukura
It is necessary to know the appropriate bacterial culture temperature and stock temperature to carry out high-precision preservative efficacy tests. In this study, we investigated whether the temperature conditions within the culture or stock affect the survivability or growth activity of Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. We found that the cold stocking condition affected the survivability of P. aeruginosa, but not that of E. coli and S. aureus. Furthermore, stocking temperature affects the growth activity of E...
2022: Biocontrol Science
https://read.qxmd.com/read/36216567/effect-of-the-cleanliness-of-spray-nozzle-on-the-concentration-of-microorganisms-in-the-spray-water-in-warm-water-bidet-toilet-seats
#11
JOURNAL ARTICLE
Toru Iyo, Keiko Asakura, Kazuyuki Omae
Bidet-toilets are widely used in households and public spaces in Japan. The effect of nozzle cleanliness on spray water quality of warm-water bidet toilet seats is unknown. We conducted a long-term experimental model-based study (2015-2016) and two survey-based studies (A: 2018-2019 and B: 2020-2021) at a university campus. Survey A measured the effect of nozzle cleansing on total viable count (TVC) and heterotrophic plate counts (HPCs) in spray water. Survey B measured the total organic carbon, residual chlorine concentration, TVC, and HPC of different fractions of sprayed water...
2022: Biocontrol Science
https://read.qxmd.com/read/36216566/the-study-of-diversity-in-sporulation-among-closely-genetically-related-bacillus-cereus-strains
#12
JOURNAL ARTICLE
Ritsuko Kuwana, Ryuji Yamazawa, Kiyoshi Ito, Hiromu Takamatsu
Bacillus cereus is an important foodborne pathogenic bacterium. Although several B. cereus strains have been isolated from the environment, the differences among these strains with respect to spore formation ability and cell morphology need clarification. In this study, a phylogenetic tree was constructed based on the 16S rRNA gene sequences of nine strains of B. cereus. Spore formation and morphology of these nine strains were compared using both phase-contrast and fluorescence microscopy to create an index of the designated sporulation stages...
2022: Biocontrol Science
https://read.qxmd.com/read/36216565/evaluation-of-bactericidal-effects-of-h-2-and-o-3-filled-ultrafine-bubbles-water
#13
JOURNAL ARTICLE
Risako Morishita, Satoshi Itoh, Mariko Takeda-Morishita
Ultrafine bubbles (UFBs) are gaining attention in diverse industries as a new type of material with specific physical properties. Bactericidal activity has been reported as one of the unique properties of UFB water; however, the bactericidal activities of UFBs related to the gas type remain unclear. In particular, the bactericidal effect of hydrogen (H2 ) -filled UFB water has not been verified. Therefore, this study aimed to evaluate the bactericidal effects of H2 - or ozone (O3 ) -filled UFB water using a bacterial suspension test...
2022: Biocontrol Science
https://read.qxmd.com/read/36216564/effect-of-high-hydrostatic-pressure-treatment-combined-with-alkaline-electrolyzed-water-on-the-injury-and-growth-characteristics-of-bacterial-spores
#14
JOURNAL ARTICLE
Daisuke Hamanaka, Koki Morita
The characteristics of growth and injury of Bacillus subtilis spores whose heat resistance was reduced by the treatment of high hydrostatic pressure processing (HPP) combined with/without alkaline electrolyzed water (AlEW) were investigated. The delay in the lag phase of growth curve observed when used in combination with AlEW, especially at 50 MPa or less, and was prolonged by about 4 to 6 hours. However, the effects of temperature and time during treatment on the lag phase were not significantly different between solo-HPP and in combination with AlEW...
2022: Biocontrol Science
https://read.qxmd.com/read/36216563/mechanism-of-polyhexamethylene-biguanide-resistance-in-purpureocillium-lilacinum-strains
#15
JOURNAL ARTICLE
Yikelamu Alimu, Yoko Kusuya, Takako Yamamoto, Kana Arita, Naofumi Shigemune, Hiroki Takahashi, Takashi Yaguchi
Purpureocillium lilacinum has been recently found to contaminate a 20% (200,000 μg/mL) aqueous solution of polyhexamethylene biguanide hydrochloride (PHMB) . We aimed to elucidate the mechanism underlying the resistance of P. lilacinum to PHMB. First, we induced the PHMB-resistant (IR) strains IFM 67050 (IR) and IFM 65838 (IR) from the type strain P. lilacinum CBS 284.36T via cultivation in a medium containing high concentrations of PHMB. We then analyzed the DNA sequences via Illumina sequencing to evaluate the presence of genetic mutations in IFM 65838 (IR) ...
2022: Biocontrol Science
https://read.qxmd.com/read/35753798/impact-of-storage-conditions-and-mold-types-on-aflatoxin-b-1-concentration-in-corn-residue-used-as-dairy-feed-in-small-holder-dairy-farms-thailand
#16
JOURNAL ARTICLE
Veena Chupia, Suwimol Tangtrongsup, Arpussara Saedan, Jaturaporn Ounnunkad, Surachai Pikulkaew, Witaya Suriyasathaporn, Wasana Chaisri
The aims of this study were to determine the impact of storage practice and mold types on mold growth and aflatoxin B1 (AFB1 ) concentration in corn residue from local seed corn plants, the main roughage source of dairy farms in the northern region in Thailand. A total of 223 samples from 2 types of corn residue - dried and wet - were collected. Mold contamination was determined by spread plate technique, and aflatoxin B1 (AFB1 ) quantification was performed by a commercial enzyme-linked immunosorbent assay...
2022: Biocontrol Science
https://read.qxmd.com/read/35753797/genetic-characterization-and-antimicrobial-resistant-profiles-of-staphylococcus-aureus-isolated-from-different-food-sources
#17
JOURNAL ARTICLE
Reem Ghabbour, Amal Awad, Gamal Younis
This study determined the prevalence of Staphylococcus aureus in food of animal origin, investigated its antimicrobial susceptibility profiles and antimicrobial-resistant genes encoding resistance to methicillin (mecA), penicillin (blaZ), and vancomycin (vanA). Two hundred and sixty food samples, including raw retail milk, meat, and meat products, were obtained from local retail shops in Mansoura city, Egypt. The overall prevalence of S. aureus in the total examined food samples was 32.69% (85/260). Methicillin-resistant S...
2022: Biocontrol Science
https://read.qxmd.com/read/35753796/evaluation-of-serotyping-of-environmental-and-clinical-isolates-of-legionella-pneumophila-using-maldi-tof-ms
#18
JOURNAL ARTICLE
Kazuyuki Sogawa, Naoto Ishizaki, Takayuki Ishige, Syota Murata, Toshibumi Taniguchi, Katsunori Furuhata
Legionella pneumophila (L. pneumophila) is responsible for most Legionnaire's disease cases diagnosed worldwide. The species includes 16 serogroups, but most Legionnaire's disease cases (85.7% in Europe, 87.0% in Japan) are caused by L. pneumophila serogroup 1. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) can be used to identify the L. pneumophila serogroup. In this study, we compared three sample preparation methods that are compatible with MALDI-TOF MS: the direct colony transfer method (DCTM), on-target extraction method (OTEM), and in-tube extraction method (ITEM)...
2022: Biocontrol Science
https://read.qxmd.com/read/35753795/species-diversity-based-on-revised-systematics-of-xerophilic-aspergillus-section-restricti-isolated-from-storage-rooms-and-houses-in-japan
#19
JOURNAL ARTICLE
Ryo Hagiuda, Hisayuki Oda, Yuji Kawakami, Dai Hirose
Eighty-seven strains of Aspergillus section Restricti were isolated from five storage rooms (50 strains) and 21 houses (37 strains) between 2014 and 2020. Eleven species were identified based on their morphological characteristics and molecular phylogeny using the rRNA internal transcribed spacer (ITS) region, calmodulin (CaM), β-tubulin (benA), and RNA polymerase II second largest subunit (RPB2) sequences. A. penicillioides, which was known to cause the deterioration of cultural assets, was isolated at high frequency (73%) from the surfaces of 11 cultural assets in the storage rooms; A...
2022: Biocontrol Science
https://read.qxmd.com/read/35753794/inhibitory-effects-of-polymyxin-b-and-human-ll-37-on-the-flagellin-expression-in-vibrio-vulnificus
#20
JOURNAL ARTICLE
Shin-Ichi Miyoshi, Mika Kumagai, Ryousuke Tanida, Kohei Soda, Yuri Yoshimoto, Tamaki Mizuno
Vibrio vulnificus, an opportunistic human pathogen responsible for primary septicemia, initiates pathogenesis by attachment to the intestinal epithelial cells for which the motility by the polar flagellum plays an essential role. The proteomic analysis of outer membrane proteins showed that the treatment with the 1/2 minimum inhibitory concentration (MIC) of polymyxin B (a bacterial antimicrobial peptide) led to the reduced production of flagellin (a major component of the polar flagellum). Furthermore, the bacterial motility was inhibited in the presence of 1/2 MIC of polymyxin B...
2022: Biocontrol Science
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