Read by QxMD icon Read

Biocontrol Science

Noriko Shimasaki, Yasuhiro Nojima, Masaya Sakakibara, Ritsuko Kikuno, Chiori Iizuka, Akira Okaue, Shunji Okuda, Katsuaki Shinohara
 Recent studies have investigated the efficacy of air-cleaning products against pathogens in the air. A standard method to evaluate the reduction in airborne viruses caused by an air cleaner has been established using a safe bacteriophage instead of pathogenic viruses; the reduction in airborne viruses is determined by counting the number of viable airborne phages by culture, after operating the air cleaner. The reduction in the number of viable airborne phages could be because of "physical decrease" or "inactivation"...
2018: Biocontrol Science
Kishio Hatai, Takashi Kamada, Lik-Ming Lau, Julius Kulip, Chin-Soon Phan, Charles Santhanaraju Vairappan
 The antifungal activity of two Bornean medicinal wild gingers Plagiostachys megacarpa and Zingiber phillippsiae were examined against Lagenidium thermophilum. The most active extract was P. megacarpa at concentration of 320 µg/mL inhibiting both hyphal growth and zoospore production of L. thermophilum in 24 h. Toxicity tests were conducted using mud crab (Scylla tranquebarica) larva. Bath treatment of P. megacarpa at concentrations of 320 and 640 µg/mL for 24 h were highly effective against hyphae and zoospores of the strain and it is non-toxic to mud crab larva...
2018: Biocontrol Science
Hajime Teramura, Noriko Fukuda, Yumiko Okada, Hirokazu Ogihara
 The four types of chromogenic selective media that are commercially available in Japan were compared for establishing a Japanese standard method for detecting Cronobacter spp. based on ISO/TS 22964:2006. When assessed using 9 standard Cronobacter spp. strains and 29 non-Cronobacter strains, Enterobacter sakazakii isolation agar, ChromocultTM Enterobacter sakazakii agar, CHROMagarTM E. sakazakii, and XM-sakazakii agar demonstrated excellent inclusivity and exclusivity. Using the ISO/TS 22964:2006 method, the recovered numbers of 38 Cronobacter spp...
2018: Biocontrol Science
Xiaowen Cui, Hsu-Ming Sherman Wen, Yoshimasa Kinoshita, Shota Koishi, Chika Isowaki, Liushu Ou, Yoshimitsu Masuda, Ken-Ichi Honjoh, Takahisa Miyamoto
 Sublethally heat-injured cells of Salmonella in food can recover under favorable conditions, leading to foodborne illness. To elucidate the molecular mechanism of recovery from heat injury, the global changes in gene transcription of Salmonella Typhimurium were investigated in previous study. In this study, the functions of genes involved in phage shock response (viz., phage shock protein (psp) genes), the transcription levels of which were found in previous study to be increased during recovery from heat injury, were investigated in recovering cells...
2018: Biocontrol Science
Hiroya Okamoto, Shoko Koizumi, Hironori Shimizu, Otomi Cho, Takashi Sugita
 Malodorants in the human axilla are produced from human biogenic precursors by axillary bacterial enzymes. In the present study, we used pyrosequencing analysis to identify the axillary bacterial microbiota of 13 Japanese male subjects with cumin-like, spicy body odor (C type), and 9 with milky, skin-based body odor (M type). Anaerococcus, Corynebacterium, and Staphylococcus predominated in both C- and M-type subjects, followed by Moraxella and Peptoniphilus. These genera accounted for 96.2-99.9% of the total bacterial population, except in the microbiota of one C-type subject...
2018: Biocontrol Science
Eri Tagawa, Hidehisa Kawahara
 Most of the ice nucleation activity inhibitor reported so far are compounds processing the hydroxyl group such as the polyphenolic derivative. After examining the anti-ice nucleation activity of the purine base, the highest compound is theophylline, and the activity showed 3.80±0.32℃ at a final concentration of 0.1 mg/ml. We found that the activity of the adenine which was essential to genome information DNA was higher than that of guanine. After examining effect of adenine concentration, high activity showed 9...
2017: Biocontrol Science
Yoko Maehara, Shin-Ichi Miyoshi
 Linear alkylbenzene sulfonate (LAS) and polyoxyethylene lauryl ether (POLE) are major surfactants contained in the laundry detergents. In the present study, the antibacterial activities of the surfactants to aquatic microorganisms were compared. When freshwater samples from a small river in Okayama city were treated with each of the surfactants, only LAS showed the significant antibacterial activity. Several strains, which survived after the treatment with 2.0% LAS, were isolated and identified by sequencing of 16S rDNA...
2017: Biocontrol Science
Masayuki Ishihara, Kaoru Murakami, Koichi Fukuda, Shingo Nakamura, Masahiro Kuwabara, Hidemi Hattori, Masanori Fujita, Tomoharu Kiyosawa, Hidetaka Yokoe
 Hypochlorous acid (HOCl) solution (200 ppm, pH 6) was prepared and evaluated for their stabilities and microbicidal activities. We demonstrated that HOCl is unstable against ultraviolet (UV) light, sunshine, contact with air, and elevated temperature (≧25℃). Furthermore, in the HOCl solution, the presence of excess NH2 - or CHO-containing organic compounds such as proteins and carbohydrates, or of inorganic ions such as NO2 - , SO3 - , PO3 - , Fe2+ , Cu2+ , and CuS, resulted in the rapid consumption of HOCl by oxidation reactions, and significantly decreased the microbicidal activity of the HOCl solution against coliform bacteria and total viable cell count...
2017: Biocontrol Science
Munenori Furuta, Takayuki Nasu, Kouichi Umeki, Duc Hoang Minh, Ken-Ichi Honjoh, Takahisa Miyamoto
 The aim was to isolate Campylobacter jejuni-specific lytic phages from meats on the market in Japan. These phages were effectively isolated from 13 of 15 (86.7%) retail chicken meat samples (skin and liver) by the enrichment method using Preston Campylobacter Selective Enrichment Broth and 10 host Campylobacter strains. Among the 26 phage isolates, 14 were extracted by means of C. jejuni L26 as a host strain. Phage PHC10 showed the broadest lytic spectrum: active against 67.4% of the 46 C. jejuni strains tested...
2017: Biocontrol Science
Hidehisa Kawahara, Eri Tagawa, Chihiro Watanabe, Jun Hamada, Sayaka Hamada
 The supercooling-facilitating (SCF) activities, that is, the anti-ice nucleation activity of the hot water extracts from five types of processed food refuse was examined. The extract with the highest activity among five hot water extracts was coffee refuse, showing 1.50℃ of SCF activity at a final concentration of 0.1 mg/ml. From the hot water extract of coffee refuse, the coffee refuse extract containing various polyphenols was prepared by the ultrafiltration (less than MWCO 10,000), a solvent fractionation of ethyl acetate...
2017: Biocontrol Science
Sharon N Nuñal, Sheila Mae S Santander-DE Leon, Wei Hongyi, Adel Amer Regal, Takeshi Yoshikawa, Suguru Okunishi, Hiroto Maeda
 Natural biodegradation of heavy oil in the marine environment can be accelerated by the addition of nutrients or seeding of pre-selected microorganisms. In this study, a microcosm experiment was conducted to investigate the effects of inorganic nutrient supplementation (biostimulation) and bacterial consortium amendment (bioaugmentation) on the natural degradative processes of artificially contaminated sediment. Our results revealed that the addition of nutrients had greater effect on remediation than the addition of bacterial cells...
2017: Biocontrol Science
Rithy Chrun, Yukie Hosotani, Susumu Kawasaki, Yasuhiro Inatsu
 Fermented vegetables are common part of Cambodian diet. The food safety status for these foods has not been investigated. This study was conducted to evaluate the microbiological hazards that contaminated fermented vegetables. A total of 68 samples of fermented vegetables were purchased randomly from five wet markets in Phnom Penh. The conventional culture methods for microbiological analysis were used. Coliform bacteria (Escherichia coli, Cronobactersakazakii, and Enterobacter spp.), opportunistic non-Entrobacteriaceae, Enterococcus spp...
2017: Biocontrol Science
Kouichi Nakanishi, Akinori Kogure, Ritsuko Kuwana, Hiromu Takamatsu, Kiyoshi Ito
 Differences in the physical properties of individual cells cannot be evaluated with conventional experimental methods that are used to study groups of cells obtained from pure cultures. To examine the differences in the thermal tolerance of individual cells that are genetically identical, a method is needed to measure the thermal energy required to kill single cells. We developed a scanning thermal microscopy (SThM) system and measured the thermal conductivity of various bacterial cells, for example, spore formeing Bacillus genus and non spore-forming bacteria such as Escherichia coli...
2017: Biocontrol Science
Nao Eguchi, Kan Fujino, Khompakorn Thanasut, Motoko Taharaguchi, Masuro Motoi, Akitomo Motoi, Kenji Oonaka, Satoshi Taharaguchi
 Agaricus is known to have immunostimulatory and anti-tumor effects. However, the antiviral effects of Agaricus have not yet been examined. In the present study, the antiviral effects of an extract of Agaricus brasiliensis KA21 (AE) on the H1N1 influenza virus (PR8 strain) were investigated. The anti-influenza virus effects of AE were examined by using the plaque formation inhibition test. AE inhibited the plaque formation of PR8 in a dose-dependent manner: 98 and 50% (IC50) inhibition at 2.5 and 0.99 mg/mL, respectively...
2017: Biocontrol Science
Kazuyuki Sogawa, Masaharu Watanabe, Takayuki Ishige, Syunsuke Segawa, Akiko Miyabe, Syota Murata, Tomoko Saito, Akihiro Sanda, Katsunori Furuhata, Fumio Nomura
 Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major pathogens responsible for nosocomial infections. The presence of MRSA in a hospital is detrimental to patients and to hospital management. Thus, rapid identification of MRSA is needed. Here, we report on a prospective method to rapidly discriminate of MSSA from MRSA using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and support vector machine (SVM) analysis in 160 clinical isolates of S. aureus...
2017: Biocontrol Science
Aya Tanaka, Mariko Era, Yumeho Obata, Manami Masuda, Takayoshi Kawahara, Takahide Kanyama, Hiroshi Morita
 Acanthamoeba is found in seawater, fresh water, and soil and is an opportunistic pathogen that causes a potentially blinding corneal infection known as Acanthamoeba keratitis. The anti-amoeba activity of 9 fatty acid salts (potassium butyrate (C4K), caproate (C6K), caprylate (C8K), caprate (C10K), laurate (C12K), myristate (C14K), oleate (C18:1K), linoleate (C18:2K), and linolenate (C18:3K)) was tested on Acanthamoeba castellanii ATCC 30010 (trophozoites and cysts). Fatty acid salts (350 mM and pH 10.5) were prepared by mixing fatty acids with the appropriate amount of KOH...
2017: Biocontrol Science
Huifang Zhang, N A Wang, Yucheng Wang, Jinjie Wang, Hong Zheng, Zhihua Liu
 The subtilisin-like serine protease gene TghSS42 was cloned from biocontrol agent Trichoderma ghanense ACCC 30153. Its coding region is 1302 bp in length, encoding 433 aa with a predicted protein molecular weight of 42.5 kDa and pI of 5.53. The accession number of cDNA sequence of TghSS42 gene is KJ740359. Furthermore, the transcription of the TghSS42 gene was all up-regulated under nine different treatments by RT-qPCR analysis, and the highest transcription level of TghSS42 approached 177.29-fold at 4 h under induction with 1% (w/v) Alternaria alternata cell walls, indicating that TghSS42 could be induced by the plant or phytopathogen...
2017: Biocontrol Science
Kazuhisa Tomita, Jun Sawai
 The inhibition of microbial attachment to food is important for the prevention of cross-contamination during food processing. The effect of several chemicals that were added in an Escherichia coli growth medium on the attachment of the bacterium to lettuce was investigated. E. coli ATCC 25922, which is reportedly a useful surrogate for E. coli O157:H7 in surface attachment studies, was preincubated in a nutrient broth (NB) containing sodium chloride, potassium chloride, sodium deoxycholate, sodium linear alkylbenzene sulfonate, or sorbic acid...
2017: Biocontrol Science
Takako Nakayama, Takashi Yamazaki, Ayaka Yo, Kazuya Tone, Mohamed Mahdi Alshahni, Ryuichi Fujisaki, Koichi Makimura
 Loop-mediated isothermal amplification (LAMP) is a useful DNA detection method with high specificity and sensitivity. The LAMP reaction is carried out within a short time at a constant temperature without the need for thermal cycling. We developed a LAMP primer set for detecting a wide range of fungi by aligning the sequences of the large subunit ribosomal RNA gene of Candida albicans (Ascomycota), Cryptococcus neoformans (Basidiomycota), and Mucor racemosus (Mucorales). The threshold of C. albicans rDNA as template with our LAMP primer set was in the range of 10-100 copies per a reaction...
2017: Biocontrol Science
Haruo Mimura, Masahiro Yagi, Kazutoshi Yoshida
 We compared the TBT-resistant ability of resting cells prepared from isolates that formed colonies on nutrient agar plates containing 100 µM tributyltin (TBT) chloride, such as Photobacterium sp. TKY1, Halomonas sp. TKY2, and Photobacterium sp. NGY1, with those from taxonomically similar type strains. Photobacterium sp. TKY1 showed the highest ability among those three isolates. The number of surviving Photobacterium sp. TKY1 cells was hardly decreased after 1 h of exposure to 100 µM TBTCl, regardless of the number of resting cells in the range from 10(9...
2017: Biocontrol Science
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"