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Biocontrol Science

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https://www.readbyqxmd.com/read/28954962/microbioligical-hazard-contamination-in-fermented-vegetables-sold-in-local-markets-in-cambodia
#1
Rithy Chrun, Yukie Hosotani, Susumu Kawasaki, Yasuhiro Inatsu
 Fermented vegetables are common part of Cambodian diet. The food safety status for these foods has not been investigated. This study was conducted to evaluate the microbiological hazards that contaminated fermented vegetables. A total of 68 samples of fermented vegetables were purchased randomly from five wet markets in Phnom Penh. The conventional culture methods for microbiological analysis were used. Coliform bacteria (Escherichia coli, Cronobactersakazakii, and Enterobacter spp.), opportunistic non-Entrobacteriaceae, Enterococcus spp...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28954961/development-of-a-novel-scanning-thermal-microscopy-sthm-method-to-measure-the-thermal-conductivity-of-biological-cells
#2
Kouichi Nakanishi, Akinori Kogure, Ritsuko Kuwana, Hiromu Takamatsu, Kiyoshi Ito
 Differences in the physical properties of individual cells cannot be evaluated with conventional experimental methods that are used to study groups of cells obtained from pure cultures. To examine the differences in the thermal tolerance of individual cells that are genetically identical, a method is needed to measure the thermal energy required to kill single cells. We developed a scanning thermal microscopy (SThM) system and measured the thermal conductivity of various bacterial cells, for example, spore formeing Bacillus genus and non spore-forming bacteria such as Escherichia coli...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28954960/in-vitro-anti-influenza-virus-activity-of-agaricus-brasiliensis-ka21
#3
Nao Eguchi, Kan Fujino, Khompakorn Thanasut, Motoko Taharaguchi, Masuro Motoi, Akitomo Motoi, Kenji Oonaka, Satoshi Taharaguchi
 Agaricus is known to have immunostimulatory and anti-tumor effects. However, the antiviral effects of Agaricus have not yet been examined. In the present study, the antiviral effects of an extract of Agaricus brasiliensis KA21 (AE) on the H1N1 influenza virus (PR8 strain) were investigated. The anti-influenza virus effects of AE were examined by using the plaque formation inhibition test. AE inhibited the plaque formation of PR8 in a dose-dependent manner: 98 and 50% (IC50) inhibition at 2.5 and 0.99 mg/mL, respectively...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28954959/rapid-discrimination-between-methicillin-sensitive-and-methicillin-resistant-staphylococcus-aureus-using-maldi-tof-mass-spectrometry
#4
Kazuyuki Sogawa, Masaharu Watanabe, Takayuki Ishige, Syunsuke Segawa, Akiko Miyabe, Syota Murata, Tomoko Saito, Akihiro Sanda, Katsunori Furuhata, Fumio Nomura
 Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major pathogens responsible for nosocomial infections. The presence of MRSA in a hospital is detrimental to patients and to hospital management. Thus, rapid identification of MRSA is needed. Here, we report on a prospective method to rapidly discriminate of MSSA from MRSA using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and support vector machine (SVM) analysis in 160 clinical isolates of S. aureus...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28954958/role-of-fatty-acid-salts-as-anti-acanthamoeba-agents-for-disinfecting-contact-lens
#5
Aya Tanaka, Mariko Era, Yumeho Obata, Manami Masuda, Takayoshi Kawahara, Takahide Kanyama, Hiroshi Morita
 Acanthamoeba is found in seawater, fresh water, and soil and is an opportunistic pathogen that causes a potentially blinding corneal infection known as Acanthamoeba keratitis. The anti-amoeba activity of 9 fatty acid salts (potassium butyrate (C4K), caproate (C6K), caprylate (C8K), caprate (C10K), laurate (C12K), myristate (C14K), oleate (C18:1K), linoleate (C18:2K), and linolenate (C18:3K)) was tested on Acanthamoeba castellanii ATCC 30010 (trophozoites and cysts). Fatty acid salts (350 mM and pH 10.5) were prepared by mixing fatty acids with the appropriate amount of KOH...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28954957/subtilisin-like-serine-protease-gene-tghss42-from-trichoderma-ghanense-accc-30153-was-successfully-expressed-in-escherichia-coli-and-recombinant-protease-rtghss42-exhibited-antifungal-ability-to-five-phytopathogens
#6
Huifang Zhang, N A Wang, Yucheng Wang, Jinjie Wang, Hong Zheng, Zhihua Liu
 The subtilisin-like serine protease gene TghSS42 was cloned from biocontrol agent Trichoderma ghanense ACCC 30153. Its coding region is 1302 bp in length, encoding 433 aa with a predicted protein molecular weight of 42.5 kDa and pI of 5.53. The accession number of cDNA sequence of TghSS42 gene is KJ740359. Furthermore, the transcription of the TghSS42 gene was all up-regulated under nine different treatments by RT-qPCR analysis, and the highest transcription level of TghSS42 approached 177.29-fold at 4 h under induction with 1% (w/v) Alternaria alternata cell walls, indicating that TghSS42 could be induced by the plant or phytopathogen...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28954956/preincubation-of-escherichia-coli-atcc-25922-with-nacl-increases-its-attachment-to-lettuce-surfaces-compared-with-other-chemicals
#7
Kazuhisa Tomita, Jun Sawai
 The inhibition of microbial attachment to food is important for the prevention of cross-contamination during food processing. The effect of several chemicals that were added in an Escherichia coli growth medium on the attachment of the bacterium to lettuce was investigated. E. coli ATCC 25922, which is reportedly a useful surrogate for E. coli O157:H7 in surface attachment studies, was preincubated in a nutrient broth (NB) containing sodium chloride, potassium chloride, sodium deoxycholate, sodium linear alkylbenzene sulfonate, or sorbic acid...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28659561/detection-of-fungi-from-an-indoor-environment-using-loop-mediated-isothermal-amplification-lamp-method
#8
Takako Nakayama, Takashi Yamazaki, Ayaka Yo, Kazuya Tone, Mohamed Mahdi Alshahni, Ryuichi Fujisaki, Koichi Makimura
 Loop-mediated isothermal amplification (LAMP) is a useful DNA detection method with high specificity and sensitivity. The LAMP reaction is carried out within a short time at a constant temperature without the need for thermal cycling. We developed a LAMP primer set for detecting a wide range of fungi by aligning the sequences of the large subunit ribosomal RNA gene of Candida albicans (Ascomycota), Cryptococcus neoformans (Basidiomycota), and Mucor racemosus (Mucorales). The threshold of C. albicans rDNA as template with our LAMP primer set was in the range of 10-100 copies per a reaction...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28659560/environmental-impact-of-tributyltin-resistant-marine-bacteria-in-the-indigenous-microbial-population-of-tributyltin-polluted-surface-sediments
#9
Haruo Mimura, Masahiro Yagi, Kazutoshi Yoshida
 We compared the TBT-resistant ability of resting cells prepared from isolates that formed colonies on nutrient agar plates containing 100 µM tributyltin (TBT) chloride, such as Photobacterium sp. TKY1, Halomonas sp. TKY2, and Photobacterium sp. NGY1, with those from taxonomically similar type strains. Photobacterium sp. TKY1 showed the highest ability among those three isolates. The number of surviving Photobacterium sp. TKY1 cells was hardly decreased after 1 h of exposure to 100 µM TBTCl, regardless of the number of resting cells in the range from 10(9...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28659559/microflora-in-the-soft-tissue-of-the-pacific-oyster-crassostrea-gigas-exposed-to-the-harmful-microalga-heterosigma-akashiwo
#10
Shinkuro Takenaka, Takeshi Yoshikawa, Shusaku Kadowaki, Suguru Okunishi, Hiroto Maeda
 A marine raphidophyte Heterosigma akashiwo is a causative agent of harmful microalgal blooms, which often cause the massive mortality of aquacultured finfish. In the present study, the Pacific oyster Crassostrea gigas was reared with H. akashiwo, and effect of the microalga on filter-feeding behavior and microflora of the gastrointestinal tract was investigated. The intake of the raphidophyte cells inhibited the molluscan filter-feeding activities, suggesting the negative physiological effect of the microalgal cell contents...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28659558/biopreservative-efficacy-of-bacteriocin-bacfl31-in-raw-ground-turkey-meat-in-terms-of-microbiological-physicochemical-and-sensory-qualities
#11
Ahlem Chakchouk-Mtibaa, Slim Smaoui, Naourez Ktari, Imen Sellem, Soumaya Najah, Ines Karray-Rebai, Lotfi Mellouli
 The effect of the semi purified bacteriocin BacFL31 at 200 and 400 AU/g on the shelf life of refrigerated raw ground turkey meat was investigated. The microbiological, physicochemical, and sensory properties of the meat samples were examined during refrigerated storage. The findings indicated that BacFL31 treatments were effective (p<0.05) against the proliferation of various spoilage microorganisms and suppressed the growth of Listeria monocytogenes and Salmonella Typhimurium. The pH, % Met-MB, and TBA-RS values of the treated samples were lower (p<0...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28659557/biocontrol-science-vol-21-no-2-june-2016
#12
(no author information available yet)
There are three mistakes in Biocontrol Science, Vol.21 (2), p.81-89 (2016). The detail is as follows.
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28659556/a-novel-double-subculture-method-and-its-theory-for-the-enumeration-of-injured-cells-in-stressed-microbial-population
#13
Tetsuaki Tsuchido
 A novel double subculture method, termed DiVSaL (Differential Viabilities between Solid and Liquid media) method, for the enumeration of injured cell population of a microorganism, which occurs after some sublethal to lethal treatment, was proposed. In this method injured cells were enumerated as the differential value between viabilities determined with two different techniques, the conventional plate counting using a solid agar medium and the growth delay analysis using a liquid medium. In the former technique, the viable cell number is obtained as colony forming unit (CFU) formed on an agar medium where sublethally injured cells are as much rescued as possible...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28659555/the-effects-of-temperature-on-the-growth-and-heat-resistance-of-cronobacter-spp
#14
Shigeko Ueda
 The growth and survival of Cronobacter isolates were examined under incubation at different temperatures, and their thermal death behavior was investigated at high temperature conditions of above 50℃. Seventy three strains isolated from fresh vegetables, dried foods and soil were tested: 28 of Cronobacter sakazakii, 5 of C. dublienensis, 27 of C. malonaticus and 13 of C. turicensis. All Cronobacter strains grew and multiplied predominantly at 35 and 44℃ until 16 hours of incubation, but showed poor growth at 15℃, and no growth at 5℃...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28659554/importance-of-the-high-expression-of-proline-transporter-putp-to-the-adaptation-of-escherichia-coli-to-high-salinity
#15
Hideaki Sasaki, Daichi Sato, Akinobu Oshima
 The effect of the amount of the proline transporter PutP expression on the mechanism of adaptation of E. coli cells to high salinity was analyzed. The PutP gene derived from the E. coli expression plasmid was introduced into the E. coli cell, and a high PutP expression strain was developed. At 1.2 M NaCl culture condition, the growth of normal E. coli cells was inhibited, whereas high ProP expression cells showed growth under 2.5 M NaCl conditions. The uptake of proline by E. coli as a compatible solute and substrate for metabolization was in good accordance with those seen in cell growth...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28659553/microbial-fouling-in-a-water-treatment-plant-and-its-control-using-biocides
#16
T S Rao, Rajesh Kumar, P Balamurugan, G K Vithal
 Water treatment plants (WTP) are vital in the food, pharmaceutical and chemical process industries. This investigation describes the dense microbial fouling by microbes and organic compounds in a WTP of a heavy water producing industrial unit. On-site observations showed severe algal and bacterial growth in the various units of the WTP which are open to the atmosphere and very dense fungal fouling in the closed vacuum degasser unit. Digital and microscopic images showed that the microbial fouling problem was primarily due to a fungus...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28367872/detection-method-for-aquatic-bacteria-of-the-fingers-as-a-potential-origin-of-the-aqueous-solution-contamination
#17
Eiichi Osono, Kazumi Honda, Yuki Inoue, Yoshihiko Norose, Megumi Takahashi, Kyoko Ichimura, Chisako Kamano, Eiji Shinya, Shun Takaku, Kentaro Okamatsu, Shinya Kawamoto, Hideaki Takizawa, Hidemi Takahashi
 Aquatic bacteria were isolated from the hands of working staffs by an adapted culture protocol. When the sample solution obtained by the" glove juice method" was incubated for 3 days at room temperature, viable cell counts increased up to 10(5)-fold, and the majority of the isolated colonies were shown to be Gram-negative aquatic bacteria, which carry the risk of contaminating water. Using R2A medium, coagulase-negative staphylococci were the dominant microbes immediately after recovery from the hands. Here it was revealed that bacteria of the phylum Proteobacteria isolated from the hand can be the causative bacteria of aqueous contamination...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28367871/occurrence-of%C3%A2-cronobacter%C3%A2-spp-in-dried-foods-%C3%A2-fresh-vegetables-and-soil
#18
Shigeko Ueda
 The present study surveyed the occurrence of Cronobacter spp. in dried foods including milk powder, spices and herbs and others, and fresh vegetables commercially available in markets, and ground soil materials for the agriculture. Cronobacter spp. were isolated from 15% of 33 spice and herb samples and 3% of 36 taste foods, and these were C. turicensis, C. malonaticus, C. sakazakii and C. dubliensis. Cronobacter spp. from fresh vegetables were detected in 12% of field vegetables and 13% of hydroponic vegetables...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28367870/comparison-of-quantitative-antifungal-testing-methods-for-textile-fabrics
#19
Yasuo Imoto, Satoshi Seino, Takashi Nakagawa, Takao A Yamamoto
 Quantitative antifungal testing methods for textile fabrics under growth-supportive conditions were studied. Fungal growth activities on unfinished textile fabrics and textile fabrics modified with Ag nanoparticles were investigated using the colony counting method and the luminescence method. Morphological changes of the fungi during incubation were investigated by microscopic observation. Comparison of the results indicated that the fungal growth activity values obtained with the colony counting method depended on the morphological state of the fungi on textile fabrics, whereas those obtained with the luminescence method did not...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28367869/aflatoxin-b1-contamination-of-dairy-feeds-after-storage-in-farm-practice-in-tropical-environmen
#20
Wantanwa Mongkon, Yoshiko Sugita-Konishi, Wasana Chaisri, Witaya Suriyasathaporn
 The objective of this study was to determine the contamination of aflatoxin B1 (AF-B1) when keeping various dairy feeds in a farm environment. The study was carried out from March to May 2011 and involved 63 small holder dairy farms belonging to a single dairy cooperative in Chiang Mai province, Thailand. All feed samples used for milking cows including 4 commercial concentrates (CC1 to CC4), by-products from local corn processing factories fermented in plastic bags (SIL), and corn and cob meal or corn dust (CCD)...
2017: Biocontrol Science
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