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Human Antibodies

Marzieh Khoshbin Nazdik, Mohammad Taheri, Mir Davood Omrani, Elham Sajjadi, Shahram Arsang-Jang, Zeinab Kazaei Koohpar, Hidetoshi Inoko, Arezou Sayad
OBJECTIVES: Multiple sclerosis (MS) is an autoimmune disease involving the central nervous system (CNS) with unknown immunopathogenic mechanisms. Matrix metalloproteinase-9 (MMP-9) facilitates T-cell migration into the CNS while the tissue inhibitor matrix metalloproteinase-1 (TIMP-1) inhibits MMP-9 actions. The aim of this study was to evaluate the expression of TIMP-1 RNA and MMP-9/TIMP-1 RNA ratio in blood cells of Iranian patients with relapsing-remitting multiple sclerosis (RRMS) treated with IFNb...
September 22, 2016: Human Antibodies
Mohammad Mahdi Eftekharian, Jamshid Karimi, Mojgansadat Safe, Ahmad Sadeghian, Shiva Borzooei, Ehsan Siahpoushi
AIMS/INTRODUCTION: Since changes in some immune system indicators such as leukocyte count and neutrophil to lymphocyte ratio in the blood and Inflammation is directly in relation with the pathogenesis of type 2 diabetes, metabolic syndrome and atherosclerosis, measuring these indicators in patients with type 2 diabetes and comparing them with control group can be a suitable indicator of Inflammation and prognosis of the disease. MATERIALS AND METHODS: The current study was done on 75 patients with type 2 diabetes and 72 healthy individuals as patient and healthy group respectively...
June 8, 2016: Human Antibodies
Mohammad Mahdi Eftekharian, Masoud Sabouri Ghannad, Mohammad Taheri, Ghodratollah Roshanaei, Mehrdokht Mazdeh, Mehrnoosh Musavi, Mona Bahmani Hormoz
OBJECTIVES: Multiple sclerosis (MS) is a complicated disease which occurs due to relationship between genes and environmental factors that causes tissue damage by autoimmune mechanisms.We investigated and illustrated the hypotheses correlated to the evidence of several putative environmental risk factors for MS onset and progression in this part of Iran. MATERIALS AND METHODS: Univariate logistic regression was used to detect the effects of environmental factors on the risk of MS...
June 8, 2016: Human Antibodies
Axel R S X Dubois, Jean-Philippe Buerckert, Regina Sinner, William J Faison, Anne M Molitor, Claude P Muller
OBJECTIVE: The hybridoma technology is one of the most important advances in clinical immunology. Little is known about the differences between the antibodies produced during the in vivo immune response and those recovered in hybridoma libraries. Here, we investigate a potential fusion bias inherent to the hybridoma production process. METHODS: Transgenic rats carrying human Ig heavy and light chain loci were immunized with measles virus (MV) to generate human mAbs...
June 8, 2016: Human Antibodies
Mohammad Taheri, Shirin Nemati, Abolfazl Movafagh, Mohammad Saberi, Reza Mirfakhraie, Mohammad Mahdi Eftekharian, Shahram Arsang-Jang, Amir Rezagholizadeh, Arezou Sayad
BACKGROUND: Multiple sclerosis (MS) as an autoimmune disorder in which the insulating covers of neurons in the Central Nervous System are destructed. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is an immunomodulatory molecule to protect against T cells hyper activation. METHODS: In this Case-control study, we compare TRAIL gene expression in peripheral blood between 50 relapse remitting MS patients and 50 healthy controls by TaqMan Real time PCR...
May 20, 2016: Human Antibodies
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December 23, 2015: Human Antibodies
(no author information available yet)
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December 23, 2015: Human Antibodies
Sanam Sadreddini, Mehrnosh Seifi-Najmi, Babollah Ghasemi, Hossein Samadi Kafil, Vahideh Alinejad, Sevil Sadreddini, Vahid Younesi, Farhad Jadidi-Niaragh, Mehdi Yousefi
BACKGROUND: Tetanus neurotoxin (TeNT) is composed of a light (LC) and heavy chain (HC) polypeptides, released by anaerobic bacterium Clostridium tetani and can cause fatal life-threatening infectious disease. Toxin HC and LC modules represents receptor binding and zinc metalloprotease activity, respectively. The passive administration of animal-derived antibodies against tetanus toxin has been considered as the mainstay therapy for years. However, this treatment is associated with several adverse effects due to the presence of anti-isotype antibodies...
December 23, 2015: Human Antibodies
Amjad Hayat Khan, Esmaeil Sadroddiny
Monoclonal antibodies (mAbs) are the leading class of targeted therapeutics and remarkably effective in addressing autoimmune diseases, inflammations, infections, and various types of cancer. Several mAbs approved by US food and drug administration (FDA), are available on the market and a number are pending for approval. Luckily, FDA approved mAbs have played a pivotal role in the treatment and prevention of lethal diseases. However, claiming that licensed mAbs are 100% safe is still debatable, because infections, malignancies, anaphylactoid, and anaphylactic reactions are the more frequently associated adverse events...
December 23, 2015: Human Antibodies
A N Okwesili, F U Onuigwe, K Ibrahim, H Buhari, A Ibrahim, H Jafaru, O Erhabor, F U Onuigwe, Z Isaac, M H Ahmed, M Y Mainasara, T C Adias, M H Yeldu, E K Uko, F Udoma
BACKGROUND: Hepatitis B (HB) is a serious global public health problem that put health professionals particularly at risk. OBJECTIVE: The aim of this study was to investigate the prevalence of Hepatitis B surface antigen (HBsAg) among Biomedical Students of African descent attending Usmanu Danfodiyo University Sokoto in North-Western Nigeria. METHODS: The Onsite HBsAg (CTK Biotech, USA) was used to detect the presence of hepatitis B surface antigen...
December 23, 2015: Human Antibodies
Andrew Hiatt, Michael Pauly, Kevin Whaley, Xiangguo Qiu, Gary Kobinger, Larry Zeitlin
This review describes the history of Ebola monoclonal antibody (mAb) development leading up to the recent severe Ebola outbreak in West Africa. The Ebola virus has presented numerous perplexing challenges in the long effort to develop therapeutic antibody strategies. Since the first report of a neutralizing human anti-Ebola mAb in 1999, the straightforward progression from in vitro neutralization resulting in in vivo protection and therapy has not occurred. A number of mAbs, including the first reported, failed to protect non-human primates (NHPs) in spite of protection in rodents...
December 23, 2015: Human Antibodies
Somanath Kallolimath, Herta Steinkellner
Human immunoglobulins circulate as highly heterogeneously glycosylated mixture of otherwise homogeneous protein backbones. A series of studies, mainly on IgG, have unequivocally proven that antibodies modulate their effector function through sugars present in the Fc domain. However, our limited technology in producing complex proteins such as antibodies, with defined glycan structures hamper in depths studies. This review introduces a plant based expression platform enabling engineering of antibody glycans...
December 23, 2015: Human Antibodies
Vikram Virdi, Paloma Juarez, Ann Depicker
BACKGROUND: Antibodies for human clinical applications are predominantly produced in mammalian expression systems, with Chinese hamster ovary (CHO) cells being the gold standard. CHO cells are ideal for the manufacturing of the IgG class of antibodies, but not for the production of complex antibodies like secretory IgAs (SIgAs) and IgMs, which remain unavailable for clinical use. In contrast, plant seeds and leaves hold the promise to produce SIgAs, IgMs and similar complex antibody formats to scalable amounts...
December 23, 2015: Human Antibodies
Mohammad Mahdi Eftekharian, Mehrnoush Mousavi, Mona Bahmani Hormoz, Ghodratollah Roshanaei, Mehrdokht Mazdeh
AIM: Multiple sclerosis is a demyeliting autoimmune inflammatory disorder of central nervous system (CNS) with unknown etiology, which afflicts more than 2.5 millions of the world's population. The aim of this study was to investigate the association between some immunological-related risk factors (tonsillectomy, appendectomy and hepatitis B vaccination) and multiple sclerosis in 2014 in Hamadan city, west of Iran. METHODS: As a case-control study, data of filled questionnaire with written consents from 250 patients and 250 age and sex matched controls, were collected and analyzed using SPSS version 16 software and Logistic regression test...
2014: Human Antibodies
Lior Sagi, Sharon Baum, Ori Barzilai, Maya Ram, Nicola Bizzaro, Marielle SanMarco, Yehuda Shoenfeld, Yaniv Sherer
BACKGROUND: Pemphigus and bullous pemphigoid are two autoimmune diseases that have similar pathogenesis. Both have a genetic predisposition, which promotes the production of auto antibodies targeted against different components of the epidermal desmosome and hemidesmosome. Antiphospholipid antibodies (aPL) are heterogeneous group of antibodies found in patients with autoimmune diseases and inflammatory conditions and are associated with thrombotic events. OBJECTIVE: We sought to determine the expression profile of eight non classical aPLs in ABD patients...
2014: Human Antibodies
Hamideh Rouhani Nejad, Jalil Fallah Mehrabadi, Jamile Nourozi, Mehdi Mahdavi
BACKGROUND: Over the last three decades, monoclonal antibodies have become powerful therapeutics and diagnostics tools. Progress in the antibody engineering, and the appearance of great selection technology such as phage display has made human antibodies production possible against antigens with high affinities. OBJECTIVE: The purpose of this study was to construct an immune antibody library from a vaccinated donor against tetanus toxin. METHODS: A blood sample was drawn from the donor who was vaccinated with tetanus toxoid...
2014: Human Antibodies
Behzad Baradaran, Jafar Majidi, Safar Farajnia, Jaleh Barar, Yadollah Omidi
Tumor growth and progression depends largely on the activity of cell membrane receptors like epidermal growth factor receptor (EGFR) that plays a pivotal role in the progression and invasion of different solid tumors. As one of the most promising approaches for targeting and therapy, monoclonal antibodies (mAbs) have widely been used in the treatment of various malignancies. However, the clinical effects of mAbs appear to be dependent upon its specificity and potency. In the current investigation, a series of mAbs were produced against human EGFR using hybridoma technology...
2014: Human Antibodies
Fatemeh Ezzatifar, Jafar Majidi, Behzad Baradaran, Leili Aghebati Maleki, Jalal Abdolalizadeh, Mehdi Yousefi
OBJECTIVE: Immunoglobulin A (IgA) is the second immunoglobulin in human serum. Monoclonal antibodies have many potential uses in diagnosis, treatment and purification. The conjugated monoclonal antibodies against human IgA are utilized in diagnostic kits. METHODS: For production of monoclonal antibodies against human Immunoglobulin A, spleen cells of the immune mouse were fused with SP2/0 as myeloma cells and Poly Ethylene Glycol (PEG). Supernatant of hybridoma cells was screened to determine the antibody by ELISA...
2014: Human Antibodies
Mohsen Naseri, Mohammad Moazzeni, Ali Akbar Purfathollah, Rahbarizadeh Fatemeh
BACKGROUND: Dissociation constant (Kd) is of major significance in immunoassay. Since affinity may be influenced by the immunoassay methodology it is important to determine this parameter under the condition of the assay used. OBJECTIVE: To employ a rapid and simple ELISA- based method for measuring dissociation constants of two Alkaline Phosphatase (ALP) specific MAbs (A1G8F7 and A1G9G3) established in our laboratory. METHODS: A simple and rapid method on enzyme- linked immunosorbent assay (ELISA) was developed for measuring the dissociation constant of antibody – antigen reactions...
2014: Human Antibodies
Karen Roye-Green, Rohan Willis, Nathlee Mc Morris, Jacqueline Dawson, Dwight Whittle, Everard Barton, Monica Smikle
BACKGROUND: The prevalence and characteristics of autoimmune hepatitis (AIH) in black populations are not well documented. OBJECTIVES: To describe the clinical and laboratory features of AIH in patients presenting at a Jamaican hospital, 1969-2009. METHODS: A retrospective review of hospital records was done and patients were classified by the revised international scoring system. RESULTS: Fifty patients satisfied criteria for diagnosis of AIH type-1 and most presented late at hospital...
2013: Human Antibodies
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