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Human Antibodies

journal
https://www.readbyqxmd.com/read/29689715/antibody-fusion-proteins-with-human-ribonucleases-1-to-8
#1
Xenia Wezler, Stefan Dübel, Thomas Schirrmann
ImmunoRNases combine tumor targeting by antibodies with the cytotoxic action of ribonucleases from the RNase A superfamily. This study investigated for the first time all catalytic active human RNase A family members (1 to 8) as effector components of antibody fusion proteins. ImmunoRNase fusion proteins were constructed using the CD30-specific bivalent recombinant scFv-Fc antibody SH313-B5. Production of the resulting entirely human immunoRNases 1 to 8 was done in mammalian cells by secretion of active forms...
April 10, 2018: Human Antibodies
https://www.readbyqxmd.com/read/29689714/production-and-characterization-of-anti-human-igg-f-ab-2-antibody-fragment
#2
Zahra Valedkarimi, Hadi Nasiri, Leili Aghebati-Maleki, Jalal Abdolalizadeh, Mojghan Esparvarinha, Jafar Majidi
In present study an optimized protocol for the separation of antibodies into antigen-binding fragments F(ab')2 using pepsin digestion was investigated. The production of these fragments is a consequential step in the development of medical research, treatment and diagnosis. For production of polyclonal antibody rabbit received antigen in four steps. The rabbit serum at 1/128000 dilution showed high absorbance in reaction with human IgG at the designed ELISA method. Rabbit IgG was purified by Ion-Exchange Chromatography (IEC) method...
April 10, 2018: Human Antibodies
https://www.readbyqxmd.com/read/29689713/evaluation-of-antibodies-to-cytomegalovirus-and-epstein-barr-virus-in-patients-with-autism-spectrum-disorder
#3
Shahla Valayi, Mohammad Mahdi Eftekharian, Mohammad Taheri, Mohammad Yousef Alikhani
Autism is a neurodevelopmental disease that manifested by a wide range of behavioral disorders. Although the etiology of autism is remained unknown but it is suggested that ASD have a complex etiology, including genetic and environmental factors, which may explain the observed different behavioral disorders in these patients. One of the proposed reasons for autism is viral infection in the early stages of development. The mechanism by which viral infection could lead to autism is still unclear.Previous studiesemphasized on the role of family membersof Herpesviruses in autism susceptibility...
April 10, 2018: Human Antibodies
https://www.readbyqxmd.com/read/29439321/knowledge-attitude-and-practice-of-hiv-aids-among-workers-at-china-first-highway-engineering-company-at-bilibila-sokota-road-project-sokota-ethiopia
#4
Mebratu Tarekegne, Addis Adera, Kalab Haile, Derara Guyo
OBJECTIVE: The aim of this study was to assess Bilbila Sokota Road Project workers' knowledge, attitude and practice on HIV/AIDS in China first highway engineering Company. METHODS: A cross sectional Survey was conducted among workers in China first high way engineering Company at Bilbila Sokota road project, Sokota, Ethiopia. Fifty-five workers were taken from project site/company. Data was collected using a validated self-administrated standardized questionnaire on Knowledge, attitude and practice including behavioral change regarding HIV/AIDS...
February 5, 2018: Human Antibodies
https://www.readbyqxmd.com/read/29439320/of-patents-and-patent-disputes-the-tnf%C3%AE-patent-files-part-2-enbrel-remicade-cimzia-and-simponi
#5
Ulrich Storz
This article is the second part of a trilogy that discusses IP issues related to anti-Tumor Necrosis factor α (TNFα) biologics. TNFα is the world's most valuable target, with accumulated sales of TNFα biologics of 34 bn USD in 2014. While in the first part of this trilogy, Humira was discussed, this second parts discusses the patent strategies of Enbrel, Remicade, Cimzia and Simponi.
February 5, 2018: Human Antibodies
https://www.readbyqxmd.com/read/29171990/development-of-nanoparticle-adjuvants-to-potentiate-the-immune-response-against-diphtheria-toxoid
#6
Fatimah M Alshanqiti, Saad Berki Al-Masaudi, Ahmed M Al-Hejin, Nawal Abd El-Baky, Elrashdy M Redwan
BACKGROUND: Over the years, diphtheria was known as contagious fatal infection caused by Corynebacterium diphtheria that affects upper respiratory system. The spread of diphtheria epidemic disease is best prevented by vaccination with diphtheria toxoid vaccine. Aluminum adjuvants were reported to stimulate the immune responses to killed and subunit vaccines. OBJECTIVE: Our study aimed to minimize adjuvant particles size, to gain insight of resulting immunity titer and impact on immune response antibody subtypes...
February 5, 2018: Human Antibodies
https://www.readbyqxmd.com/read/29036807/obtaining-and-characterization-of-monoclonal-antibodies-against-recombinant-extracellular-domain-of-human-epidermal-growth-factor-receptor-2
#7
Nurgul Sarina, Sailau Abeldenov, Aigerim Turgimbayeva, Assylbek Zhylkibayev, Yerlan Ramankulov, Bekbolat Khassenov, Saule Eskendirova
Human epidermal growth factor receptor 2 (HER2) is an important biomarker for detection and treatment of different types of cancers such as breast, ovarian, stomach cancer. In this study, we developed a monoclonal antibody against the extracellular domain (ECD) of HER2 biomarker of breast cancer. For this purpose, the ECD-HER2 gene was amplified and cloned into an expression vector. Gene was generated in Escherichia coli BL21 (DE3) strain for expression of recombinant protein. The expressed protein was separated by SDS-PAGE and detected by anti-his monoclonal antibody in immunoblotting...
February 5, 2018: Human Antibodies
https://www.readbyqxmd.com/read/29036806/pritumumab-the-first-therapeutic-antibody-for-glioma-patients
#8
Ivan Babic, Elmar Nurmemmedov, Venkata M Yenugonda, Tiffany Juarez, Natsuko Nomura, Sandeep C Pingle, Mark C Glassy, Santosh Kesari
Immunotherapy is now at the forefront of cancer therapeutic development. Gliomas are a particularly aggressive form of brain cancer for which immunotherapy may hold promise. Pritumumab (also known in the literature as CLNH11, CLN-IgG, and ACA-11) was the first monoclonal antibody tested in cancer patients. Pritumumab is a natural human monoclonal antibody developed from a B lymphocyte isolated from a regional draining lymph node of a patient with cervical carcinoma. The antibody binds ecto-domain vimentin on the surface of cancer cells...
February 5, 2018: Human Antibodies
https://www.readbyqxmd.com/read/29036805/determining-the-igm-and-igg-antibodies-titer-against-hsv1-hsv2-and-cmv-in-the-serum-of-schizophrenia-patients
#9
Masome Mohagheghi, Mohammad Mahdi Eftekharian, Mohammad Taheri, Mohammad Yousef Alikhani
Schizophrenia is a destructive clinical syndrome with diverse mental pathologies. Different mechanisms and factors have a role in this disease. A possible mechanism is that teratogenic viruses cause brain changes and results in the disease appearance. The schizophrenia patients were diagnosed by psychologists and with the consent of patients, five CC of venous blood was drawn. Than Serum samples were isolated and immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies against herpes simplex virus-1 (HSV-1), herpes simplex virus-2 (HSV-2) and cytomegalovirus (CMV) were quantified by ELISA sandwich kit...
February 5, 2018: Human Antibodies
https://www.readbyqxmd.com/read/28582853/investigating-the-exon-6-sequence-changes-of-interleukin-7-receptor-a-il7ra-gene-in-patients-with-relapsing-remitting-multiple-sclerosis
#10
Mohammad Taheri, Arezou Sayad
BACKGROUND: Interleukin 7 receptor alpha (IL7RA) gene that encodes a subunit of IL7 receptor has been reported to be associated with different immunologic disease. OBJECTIVE: Multiple Sclerosis (MS) patients have shown an aberrant blood level of soluble form of IL7R protein. The genomic changes in the sequence of this gene have been suggested to be correlated with its altered splicing specially, variants in the exon 6 of the gene have been reported to influence the maintenance or skipping of this exon and control the soluble or insoluble form of the final product...
February 5, 2018: Human Antibodies
https://www.readbyqxmd.com/read/29060935/frontiers-of-monoclonal-antibodies-applications-in-medical-practices
#11
Shridhar C Ghagane, Sridevi I Puranik, Siew Hua Gan, Murigendra B Hiremath, R B Nerli, M V Ravishankar
With the flourishing of innovation in drug discovery into a new era of personalized therapy, the use of monoclonal antibodies (mAbs) in the treatment of various ailments lies at the forefront. Major improvements in genetic sequencing and biomedical techniques as well as research into mAbs emphasize on determining new targets for advanced therapy while maximizing efficacy for clinical application. However, a balance has to be achieved concerning developing a target with low toxicity combined with high specificity and versatility, to allow a specific antibody to facilitate several biotic effects, ranging from neutralization of virus mechanisms to modulation of immune response and maintaining low global economic cost...
October 13, 2017: Human Antibodies
https://www.readbyqxmd.com/read/29036810/naturalizing-activity-and-safety-of-human-monoclonal-antibodies-against-of-hepatitis-c-virus
#12
Tawfeek H Abelhafez, Ashraf A Tabll, Mostafa K El-Awady, Mohammad M Mashaly, Reem El Shenawy, Yasmine S El-Abd, Maysa H Shaker, Camelia A Abdel Malak
AIM: Assessment of neutralizing activity of the human monoclonal antibodies against HCV and also study its safety in experimental small animals (Swiss mice). MATERIALS AND METHODS: Assessment of neutralizing activity of the human monoclonal antibodies against HCV envelope regions (E1, E2) by two methods (by HCV cc infectious system and by using positive HCV positive serum as source of HCV particles (neutralizing assay 2). Dot ELISA were used to study the activity of the generated antibodies...
September 29, 2017: Human Antibodies
https://www.readbyqxmd.com/read/29036809/role-of-fshr-rs6165-and-esr2-rs4986938-polymorphisms-in-ovarian-stimulation-of-iranian-women-who-underwent-assisted-reproduction-treatment
#13
Maryam Kaviani, Sayyed Mohammad Hossein Ghaderian, Soheila Arefi, Mehrdad Hashemi, Sarah Sadat Aghabozorg Afjeh
BACKGROUND: Gonadotropin therapy was used to stimulate the ovary in infertile women who underwent assisted reproduction treatment (ART). Numerous studies indicated that infertile women showed different responses to gonadotropin therapy. Follicle stimulating hormone receptor (FSHR) and Oestrogen receptor (ER) polymorphisms have been reported to involve induction of folliculogenesis and ovarian response to treatment. METHODS: In the present study, two polymorphisms, namely FSHR rs6165 and ESR2 rs4986938, were investigated in 198 Iranian infertile women aged less than 39 years who underwent ART...
September 29, 2017: Human Antibodies
https://www.readbyqxmd.com/read/29036808/ecotropic-viral-integration-site-5-evi5-expression-analysis-in-multiple-sclerosis-patients
#14
Mehrdokht Mazdeh, Mahnoosh Rahimi, Mohammad Mahdi Eftekharian, Mir Davood Omrani, Arezou Sayad, Mohammad Taheri, Soudeh Ghafouri-Fard
BACKGROUND: Multiple sclerosis (MS) is a complex immune-related disorder of the central nervous system (CNS) in which dysregulation of different classes of T cells are involved. Variants in Ecotropic Viral Integration Site 5 (EVI5) gene has been shown to be significantly associated with MS in different populations. OBJECTIVES: However, there is no data regarding relative expression of this gene in peripheral blood of MS patients compared with healthy controls. METHODS: In the present study we assessed expression of EVI5 in 50 Iranian MS patients compared with healthy subjects by means of quantitative real time RT-PCR...
September 8, 2017: Human Antibodies
https://www.readbyqxmd.com/read/28582852/human-anti-lipopolysaccharid-lps-antibodies-against-legionella-with-high-species-specificity
#15
Philipp Kuhn, Stefanie Thiem, Michael Steinert, Duncan Purvis, Veronika Lugmayr, Ulrich Treutlein, Lutz Plobner, Robert-Matthias Leiser, Michael Hust, Stefan Dübel
Legionella are Gram-negative bacteria that are ubiquitously present in natural and man-made water reservoirs. When humans inhale aerosolized water contaminated with Legionella, alveolar macrophages can be infected, which may lead to a life-threatening pneumonia called Legionnaires' disease. Due to the universal distribution of Legionella in water and their potential threat to human health, the Legionella concentration in water for human use must be strictly monitored, which is difficult since the standard detection still relies on lengthy cultivation and analysis of bacterial morphology...
July 19, 2017: Human Antibodies
https://www.readbyqxmd.com/read/28505966/on-the-antigen-antibody-interaction-a-thermodynamic-consideration
#16
Philip J G M Voets
Despite its relevance to many biomedical fields, relatively little effort has been put into a comprehensible quantitative description of the effect of reaction temperature on the interaction between antigens and their antibodies. In this article, a novel, straightforward mathematical model is proposed, which aims to describe the effect of temperature on antigen-antibody kinetics. The model proposed in this article could hopefully provide clinicians, immunologists, and biochemists with an improved insight into the kinetic effect of fluctuations in reaction temperature on antigen-antibody-dependent processes and therefore into the kinetics of the humoral adaptive immune response...
July 19, 2017: Human Antibodies
https://www.readbyqxmd.com/read/28505965/determining-the-igm-and-igg-antibody-titer-against-cmv-and-helicobacter-pylori-in-the-serum-of-multiple-sclerosis-patients-comparing-to-the-control-group-in-hamadan
#17
Masome Afiati Salim, Mohammad Mahdi Eftekharian, Mohammad Taheri, Mohammad Yousef Alikhani
BACKGROUND: Multiple sclerosis (MS) is a chronic autoimmune disease that disables central nervous system (CNS) system. Cytomegalovirus (CMV) probably has an important role in the MS pathology. The infection with helicobacter pylori also is recognized as a protective agent against MS in female. METHODS: Serum samples were isolated and frozen at -70∘C. The earlier mentioned anti-virus antibodies and antibacterial antibodies were quantified by Elisa kit. RESULTS: The results showed that IgG antibody average value against cytomegalovirus in the blood of multiple sclerosis patients not only decreased but also was significant statistically (p< 0...
July 19, 2017: Human Antibodies
https://www.readbyqxmd.com/read/28482631/keyword-index-volume-25-2016-2017
#18
(no author information available yet)
No abstract text is available yet for this article.
April 24, 2017: Human Antibodies
https://www.readbyqxmd.com/read/28482629/this-issue-is-dedicated-to-george-klein-1925-2016-a-giant-creative-and-stimulating-scientist-of-infinite-knowledge-friend-and-mentor
#19
(no author information available yet)
No abstract text is available yet for this article.
April 24, 2017: Human Antibodies
https://www.readbyqxmd.com/read/28128761/keyword-index-volume-24-2015-2016
#20
(no author information available yet)
No abstract text is available yet for this article.
January 17, 2017: Human Antibodies
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