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Nitric Oxide: Biology and Chemistry

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https://www.readbyqxmd.com/read/28315470/the-nitric-oxide-synthase-2-pathway-is-targeted-by-both-pro-and-anti-inflammatory-treatments-in-the-immature-human-intestine
#1
Emanuela Ferretti, Eric Tremblay, Marie-Pier Thibault, David Grynspan, Karolina M Burghardt, Marcos Bettolli, Corentin Babakissa, Emile Levy, Jean-François Beaulieu
BACKGROUND AND AIM: NO synthase 2 (NOS2) was recently identified as one the most overexpressed genes in intestinal samples of premature infants with necrotizing enterocolitis (NEC). NOS2 is widely implicated in the processes of epithelial cell injury/apoptosis and host immune defense but its specific role in inflammation of the immature human intestinal mucosa remains unclear. Interestingly, factors that prevent NEC such as epidermal growth factor (EGF) attenuate the inflammatory response in the mid-gestation human small intestine using serum-free organ culture while drugs that are associated with NEC occurrence such as the non-steroidal anti-inflammatory drug, indomethacin (INDO), exert multiple detrimental effects on the immature human intestine...
March 14, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28315469/nitric-oxide-dioxygenase-and-peroxidase-activity-of-arabidopsis-phytoglobin3-and-its-potential-role-in-pathogen-defense
#2
Nitika Mukhi, Suman Kundu, Jagreet Kaur
Phytoglobin 3 appears to be ubiquitous in plants, yet there has been dearth of evidence for their potent physiological functions. Previous crystallographic studies suggest a potent NO dioxygenase activity of Arabidopsis phytoglobin 3 (AHb3). The present work examined the in vivo function of AHb3 in plant physiology and its role in biotic stress using Arabidopsis- Sclerotinia sclerotorium pathosystem. The gene was found to be ubiquitously expressed in all plant tissues, with moderately increased expression in roots...
March 14, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28302517/exercise-training-rescues-high-fat-diet-induced-neuronal-nitric-oxide-synthase-expression-in-the-hippocampus-and-cerebral-cortex-of-mice
#3
Yuki Tomiga, Saki Yoshimura, Ai Ito, Shihoko Nakashima, Kentaro Kawanaka, Yoshinari Uehara, Hiroaki Tanaka, Yasuki Higaki
Consumption of a high fat diet (HFD) and being overweight both induce functional deterioration and atrophy of the hippocampus. These alterations are associated with mental disorders such as depression and anxiety. Exercise combats obesity and enhances brain health. There is substantial evidence that neuronal nitric oxide synthase (nNOS) is a key regulator of affective behavior, and that increased brain nNOS leads to anxiety while environmental enrichment (EE), which reduces brain nNOS, has anxiolytic effects...
March 14, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28274830/alternative-fluorimetric-based-method-to-detect-and-compare-total-s-nitrosothiols-in-plants
#4
Paulo Tamaso Mioto, Marta Rodríguez-Ruiz, Augustin Catalin Mot, Rafael Zuccarelli, Francisco J Corpas, Luciano Freschi, Helenice Mercier
Nitric oxide (NO) is an important signaling molecule occurring in virtually all organisms, whose mechanism of action relies mainly on its interaction with proteins or peptides by nitrosylation, forming compounds such as S-nitrosothiols (SNO). The Saville reaction and the ozone-based chemiluminescence method are the main techniques used for nitrosylated protein quantification. While the Saville assay is not very sensitive, the ozone-based chemiluminescence is expensive and time-consuming. Here we propose a method in which the protein-bound NO groups are exposed to UV light, cleaving the bond and allowing the quantification of the derived NO molecules by diamino-rhodamine (DAR) dyes...
March 6, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28268114/s-mansoni-t-cruzi-co-infection-modulates-arginase-1-inos-expression-liver-and-heart-disease-in-mice
#5
João Paulo Ferreira Rodrigues, Ivo Santana Caldas, Reggiani Vilela Gonçalves, Leonardo Augusto Almeida, Raquel Lopes Martins Souza, Rômulo Dias Novaes
Although Schistosoma species and Trypanosoma cruzi share common endemic areas, co-infections by these parasites remains overlooked. By using a murine model of S. mansoni and T. cruzi co-infection, we investigated if and to what extent these infections might interact to change the pathological outcomes typically observed when the host is infected by a single parasite species. Swiss mice were randomized into four groups: uninfected (NI) and those infected by S. mansoni (SM), T. cruzi (TC) or co-infected (SM + TC)...
March 4, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28267592/sfrp4-signalling-of-apoptosis-and-angiostasis-uses-nitric-oxide-cgmp-permeability-axis-of-endothelium
#6
Uttara Saran, Mani Krishna Priya, Uma Maheswari Balaguru, Akila Swaminathan, Shunmugam Nagarajan, Arun Dharmarajan, Suvro Chatterjee
Nitric oxide (NO) plays a critical role in endothelial functions such as cellular migration, vascular permeability and angiogenesis. Angiogenesis, the formation of new blood vessels from "pre-existing" ones is a carefully regulated process and essential during reproduction, development and wound healing. Previously our lab group reported that Secreted Frizzled-Related Protein 4 (sFRP4) could inhibit angiogenesis in both in vitro and in vivo conditions. sFRP4 belongs to a family of secreted glycoproteins that function as antagonists of the canonical Wnt signalling pathway...
March 3, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28257997/carbon-monoxide-does-not-contribute-to-vascular-tonus-improvement-in-exercise-trained-rats-with-chronic-nitric-oxide-synthase-inhibition
#7
Seher Nasırcılar Ülker, Günnur Koçer, Ümit Kemal Şentürk
Carbon monoxide (CO), an end product of heme oxygenase (HO) that is involved in the regulation of vascular tonus, may show a compensatory effect in nitric oxide (NO) deficiency. This study aimed to assess the effect of the HO/CO system on the vascular tone in exercise-trained rats with hypertension induced by chronic NO synthase (NOS) inhibition. Hypertension was induced by N-nitro-l-arginine methyl ester (25 mg/kg/day in drinking water), and exercise training comprised swimming 1 h/day, 5 days/week, for 6 weeks...
March 1, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28257996/linking-of-mpges-1-and-inos-activates-stem-like-phenotype-in-egfr-driven-epithelial-tumor-cells
#8
Erika Terzuoli, Federica Finetti, Filomena Costanza, Antonio Giachetti, Marina Ziche, Sandra Donnini
Inflammatory prostaglandin E-2 (PGE-2) favors cancer progression in epithelial tumors characterized by persistent oncogene input. However, its effects on tumor cell stemness are poorly understood at molecular level. Here we describe two epithelial tumor cells A431 and A459, originating from human lung and skin tumors, in which epithelial growth factor (EGF) induces sequential up-regulation of mPGES-1 and iNOS enzymes, producing an inflammatory intracellular milieu. We demonstrated that concerted action of EGF, mPGES-1 and iNOS causes sharp changes in cell phenotype demonstrated by acquisition of stem-cell features and activation of the epithelial-mesenchymal transition (EMT)...
March 1, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28238751/drastic-decrease-of-global-l-arginine-bioavailability-during-orthotopic-liver-transplantation-a-matter-of-atp-deficiency-of-the-graft
#9
LETTER
Dimitrios Tsikas, Erik Hanff, Thomas Becker
No abstract text is available yet for this article.
February 23, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28235634/platelet-inhibition-and-increased-phosphorylated-vasodilator-stimulated-phosphoprotein-following-sodium-nitrite-inhalation
#10
Tipparat Parakaw, Kran Suknuntha, Pornpun Vivithanaporn, Axel Schlagenhauf, Supachai Topanurak, Suthat Fucharoen, Kovit Pattanapanyasat, Alan Schechter, Nathawut Sibmooh, Sirada Srihirun
In the presence of red blood cells (RBCs), nitrite inhibits platelets through its conversion to nitric oxide (NO) by the reductase activity of partially deoxygenated hemoglobin. Inhaled sodium nitrite is being investigated as a therapy for pulmonary hypertension. Here, we measured platelet aggregation, P-selectin expression, platelet-leukocyte aggregates and phosphorylated vasodilator-stimulated phosphoprotein (P-VASP(Ser239)) following sodium nitrite inhalation in healthy subjects. In vitro incubation of nitrite with deoxygenated whole blood showed an increase in P-VASP(Ser239), which was inhibited by ODQ, a soluble guanylyl cyclase (sGC) inhibitor...
February 21, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28232085/angiotensin-ii-dependent-cardiac-remodeling-in-the-eel-anguilla-anguilla-involves-the-nos-no-system
#11
Mariacristina Filice, Daniela Amelio, Filippo Garofalo, Sabrina David, Alberto Fucarino, Frank Bo Jensen, Sandra Imbrogno, Maria Carmela Cerra
Angiotensin II (AngII), the principal effector of the Renin-Angiotensin System (RAS), plays an important role in controlling mammalian cardiac morpho-functional remodelling. In the eel Anguilla anguilla, one month administration of AngII improves cardiac performance and influences the expression and localization of molecules which regulate cell growth. To deeper investigate the morpho-functional chronic influences of AngII on the eel heart and the molecular mechanisms involved, freshwater eels (A. anguilla) were intraperitoneally injected for 2 months with AngII (1 nmol g BW(-1))...
February 20, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28216239/interaction-between-nitric-oxide-signaling-and-gap-junctions-during-ischemic-preconditioning-importance-of-s-nitrosylation-vs-protein-kinase-g-activation
#12
REVIEW
Fei Xie, Bing Rong, Tian-Cheng Wang, Li Hao, Ming-Jie Lin, Jing-Quan Zhong
Much effort has been dedicated to exploring the mechanisms of IPC, and the GJ is one of the proposed targets of IPC. Several lines of evidence have indicated that NO affects GJ permeability regulation and expression of connexin isoforms. NO-induced stimulation of the sGC-cGMP pathway and the subsequent PKG activation could lead directly to connexin phosphorylation and GJ coupling modification. Additionally, because NO-induced cardioprotection against I/R injury beyond the cGMP/PKG-dependent pathway has been reported in isolated cardiomyocytes, it has been posited that NO-mediated GJ coupling might be independent from the activation of the NO-induced cGMP/PKG pathway during IPC...
February 12, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28216238/dinitrosyl-iron-complexes-with-natural-thiol-containing-ligands-in-aqueous-solutions-synthesis-and-some-physico-chemical-characteristics-a-methodological-review
#13
REVIEW
Anatoly F Vanin, Rostislav R Borodulin, Vasak D Mikoyan
Two approaches to the synthesis of dinitrosyl iron complexes (DNIC) with glutathione and l-cysteine in aqueous solutions based on the use of gaseous NO and appropriate S-nitrosothiols, viz., S-nitrosoglutathione (GS-NO) or S-nitrosocysteine (Cys-NO), respectively, are considered. A schematic representation of a vacuum unit for generation and accumulation of gaseous NO purified from the NO2 admixture and its application for obtaining aqueous solutions of DNIC in a Thunberg apparatus is given. To achieve this, a solution of bivalent iron in distilled water is loaded into the upper chamber of the Thunberg apparatus, while the thiol solution in an appropriate buffer (рН 7...
February 12, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28193486/post-translational-modifications-of-medicago-truncatula-glutathione-peroxidase-1-induced-by-nitric-oxide
#14
Claude Castella, Ioanna Mirtziou, Aurélie Seassau, Alexandre Boscari, Françoise Montrichard, Kalliopi Papadopoulou, Nicolas Rouhier, Alain Puppo, Renaud Brouquisse
Plant glutathione peroxidases (Gpx) catalyse the reduction of various peroxides, such as hydrogen peroxide (H2O2), phospholipid hydroperoxides and peroxynitrite, but at the expense of thioredoxins rather than glutathione. A main function of plant Gpxs is the protection of biological membranes by scavenging phospholipid hydroperoxides, but some Gpxs have also been associated with H2O2 sensing and redox signal transduction. Nitric oxide (NO) is not only known to induce the expression of Gpx family members, but also to inhibit Gpx activity, presumably through the S-nitrosylation of conserved cysteine residues...
February 11, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28185882/s-transnitrosation-reactions-of-hydrogen-sulfide-h2s-hs-s-2-with-s-nitrosated-cysteinyl-thiols-in-phosphate-buffer-of-ph-7-4-results-and%C3%A2-review-of-the-literature
#15
Dimitrios Tsikas, Anke Böhmer
Cysteine (CysSH) and its derivatives including N-acetylcysteine (NAC) and glutathione (GSH), and cysteine residues in proteins and enzymes are nitrosated with nitric oxide (NO) reaction products such as N2O3 to form S-nitrosated cysteine thiols (RCysSNO). RCysSNO undergo with cysteine thiols (RCysSH) S-transnitrosation reactions, thereby transferring reversibly their nitrosyl ((+)NO) group to RCysSH to form RCysSNO. (•)NO release from RCysSNO and S-transnitrosation are considered the most important features and signalling pathways of RCysSNO...
February 6, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28167162/disparate-response-to-microoxia-and-nitrogen-oxides-of-the-bradyrhizobium-japonicum-napedabc-nirk-and-norcbqd-denitrification-genes
#16
Emilio Bueno, Eloy F Robles, María J Torres, Tino Krell, Eulogio J Bedmar, María J Delgado, Socorro Mesa
Expression of the Bradyrhizobium japonicum napEDABC, nirK and norCBQD denitrification genes requires low oxygen (O2) tension and nitrate (NO3(-)), through a regulatory network comprised of two coordinated cascades, FixLJ-FixK2-NnrR and RegSR-NifA. To precisely understand how these signals are integrated in the FixLJ-FixK2-NnrR circuit, we analyzed β-Galactosidase activities from napE-lacZ, nirK-lacZ and norC-lacZ fusions, and performed analyses of NapC and NorC levels as well as periplasmic nitrate reductase (Nap) activity, in B...
February 3, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28137611/analgesic-effect-of-dimethyl-trisulfide-in-mice-is-mediated-by-trpa1-and-sst4-receptors
#17
Gábor Pozsgai, Maja Payrits, Éva Sághy, Réka Sebestyén-Bátai, Elise Steen, Éva Szőke, Zoltán Sándor, Margit Solymár, András Garami, Péter Orvos, László Tálosi, Zsuzsanna Helyes, Erika Pintér
TRPA1 receptors are calcium-permeable ligand-gated channels expressed in primary sensory neurons and involved in inflammation and pain. Activation of these neurons might have analgesic effect. Suggested mechanism of analgesic effect mediated by TRPA1 activation is the release of somatostatin (SOM) and its action on sst4 receptors. In the present study analgesic effect of TRPA1 activation on primary sensory neurons by organic trisulfide compound dimethyl trisulfide (DMTS) presumably leading to SOM release was investigated...
January 28, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28109803/shedding-light-on-no-homeostasis-light-as-a-key-regulator-of-glutathione-and-nitric-oxide-metabolisms-during-seedling-deetiolation
#18
Rafael Zuccarelli, Aline C P Coelho, Lazaro E P Peres, Luciano Freschi
Despite the significant impacts of light on nitric oxide (NO) levels in plants, the mechanism underlying the influence of this environmental factor on NO metabolism remains poorly understood. A critical mechanism controlling NO levels in plant cells relies on the S-nitrosylation of glutathione (GSH), giving rise to S-nitrosoglutathione (GSNO), which can be either stored or degraded depending on the cellular context. Here, we demonstrate that a strict balance is maintained between NO generation and scavenging during tomato (Solanum lycopersicum) seedling deetiolation...
January 18, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28062279/nitric-oxide-induced-synthesis-of-hydrogen-sulfide-alleviates-osmotic-stress-in-wheat-seedlings-through-sustaining-antioxidant-enzymes-osmolyte-accumulation-and-cysteine-homeostasis
#19
M Nasir Khan, M Mobin, Zahid Khorshid Abbas, Manzer H Siddiqui
Nitric oxide (NO) and hydrogen sulfide (H2S) have been shown to act as signaling molecules in various physiological processes, play significant roles in plant cellular processes, and also mediate responses to both biotic and abiotic stresses in plants. The present investigation was carried out to test the effect of exogenous NO on endogenous synthesis of H2S in osmotic-stressed wheat (Triticum aestivum L.) seedlings. The results show that application of NO to wheat seedlings, suffered from PEG8000-induced osmotic stress, considerably enhanced the activities of H2S-synthesizing enzymes l-cysteine desulfhydrase (LCD) and d-cysteine desulfhydrase (DCD) leading to enhanced level of endogenous H2S content...
January 3, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28235635/cephalosporin-no-donor-prodrug-pyrro-c3d-shows-%C3%AE-lactam-mediated-activity-against-streptococcus-pneumoniae-biofilms
#20
Raymond N Allan, Michael J Kelso, Ardeshir Rineh, Nageshwar R Yepuri, Martin Feelisch, Odel Soren, Sanjita Brito-Mutunayagam, Rami J Salib, Paul Stoodley, Stuart C Clarke, Jeremy S Webb, Luanne Hall-Stoodley, Saul N Faust
Bacterial biofilms show high tolerance towards antibiotics and are a significant problem in clinical settings where they are a primary cause of chronic infections. Novel therapeutic strategies are needed to improve anti-biofilm efficacy and support reduction in antibiotic use. Treatment with exogenous nitric oxide (NO) has been shown to modulate bacterial signaling and metabolic processes that render biofilms more susceptible to antibiotics. We previously reported on cephalosporin-3'-diazeniumdiolates (C3Ds) as NO-donor prodrugs designed to selectively deliver NO to bacterial infection sites following reaction with β-lactamases...
May 1, 2017: Nitric Oxide: Biology and Chemistry
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