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Clinical Hemorheology and Microcirculation

Giuseppe Di Stolfo
BACKGROUND: Carotid stenting stimulates intimal proliferation through platelet and stem cell activation. OBJECTIVE: The aim of this study is to evaluate whether the administration before or after carotid stenting of clopidogrel loading dose may play a role on circulating endothelial progenitor cells, stromal cell-derived factor-1α (SDF-1α) and neointimal hyperplasia. METHODS: We recruited 13 patients (aged 74.52±7.23) with indication of carotid revascularization and in therapy with salicylic acid and statin...
September 29, 2019: Clinical Hemorheology and Microcirculation
David Astapenko, Vlasta Dostalova, Vlasta Dostalova, Jaroslav Kraus, Vera Radochova, Pavel Dostal, Alena Ticha, Radomir Hyspler, Christian Lehmann, Vladimir Cerny
BACKGROUND AND OBJECTIVE: The endothelial glycocalyx (EG) is fragile and sensitive to damage such as exposure to hypernatremia. Our aim was to describe the influence of hypernatremia on the EG in sublingual and brain microcirculation in rabbits. METHODS: Hypernatremia was induced by intravenous administration of 10% NaCl solution. The sublingual and brain microcirculation were evaluated by the Side-stream Dark Field imaging before (T1) and 20 minutes after infusion of 10% saline (T2)...
November 2, 2018: Clinical Hemorheology and Microcirculation
Juliane Rieger, Carsten Hopperdietzel, Sabine Kaessmeyer, Ilka Slosarek, Sebastian Diecke, Ken Richardson, Johanna Plendl
BACKGROUND: Human and equine patients are known to frequently develop vascular complications, particularly thrombosis both in veins and arteries as well as in the microvasculature. OBJECTIVE: The aim of the present study was to investigate and compare the angiogenic response of human and equine endothelial cells to lesions in an in vitro scratch assay. METHODS: Endothelial cells from human umbilical vein (HUVEC), abdominal aorta (HAAEC) and dermal microvasculature (HDMEC) as well as equine carotid artery (EACEC) and jugular vein (EVJEC) were cultured and an elongated defect was created (scratch or "wound")...
October 29, 2018: Clinical Hemorheology and Microcirculation
Philipp Kauffmann, Markus Troeltzsch, Phillipp Brockmeyer, Hanibal Bohnenberger, Marietta Manzke, Canis Martin, Suntharalingam Gaayathiri, Henning Schliephake, Lukas Prantl, Thiha Aung
INTRODUCTION: The oral squamous cell carcinoma (OSCC) is a leading cause of death in human malignancies. The aim of this study is to integrate the CAM Assay as a reliable and good working in vivo model for the evaluation of OSCC tumor samples and its growth into the clinical work flow. MATERIAL AND METHODS: Fresh human Tumor samples (OSCCs) 1×1 cm in size were cut into 350-450μm thick slices by a Vibratome and put on the prepared CAM model.After growth of the tumor tissue on the CAM, we started with topical induction of proinflammatory cytokines (TNFα) and growth factors (TGFβ)...
October 29, 2018: Clinical Hemorheology and Microcirculation
Ute Probst, Dominik Sieron, Karin Bruenn, Irene Fuhrmann, Niklas Verloh, Christian Stroszczynski, Ernst-Michael Jung, Philipp Wiggermann, Michael Haimerl
PURPOSE: Contrast enhanced magnetic resonance imaging (MRI) is able to assess liver function by characteristic changes of signal intensity (SI).The aim was to evaluate dynamic contrast-enhanced SI-indices of the abdominal aorta, portal vein and liver. METHODS: 72 patients underwent Gd-EOB-DTPA-enhanced MRI and a 13C-methacetin-based liver breath test (13C-MBT) for evaluation of liver function. Region-of-interest measurements in the liver, abdominal aorta and portal vein during native, arterial (AP), late arterial (LAP), portal venous (PVP) and hepatobiliary phase (HBP) were applied to analyze SI-indices in T1-weighted volume-interpolated breath-hold examination (VIBE) sequences with fat-suppression and relative enhancement (RE) analysis was performed...
October 22, 2018: Clinical Hemorheology and Microcirculation
Jie Zou, Weiwei Wang, Karl Kratz, Xun Xu, Yan Nie, Nan Ma, Andreas Lendlein
Polycarbonate (PC) substrate is well suited for culturing human mesenchymal stem cells (MSCs) with high proliferation rate, low cell apoptosis rate and negligible cytotoxic effects. However, little is known about the influence of PC on MSC activity including senescence, differentiation and secretion. In this study, the PC cell culture insert was applied for human MSC culture and was compared with polystyrene (PS) and standard tissue culture plate (TCP). The results showed that MSCs were able to adhere on PC surface, exhibiting a spindle-shaped morphology...
October 20, 2018: Clinical Hemorheology and Microcirculation
Irene Fuhrmann, Karin Brünn, Ute Probst, Niklas Verloh, Christian Stroszczynski, Ernst Michael Jung, Philipp Wiggermann, Michael Haimerl
BACKGROUND: The diagnostic value of Doppler ultrasonography of the portal vein for the evaluation of liver function is still contradictory. OBJECTIVE: Aim of this study was to test the relationship between clinical liver function tests based on MRI and breath testing and blood flow in the portal vein. METHODS: The portal vein velocity was measured by color coded Doppler ultrasonography (CCDS) and tested against the relative enhancement (RE), a MRI-based index of liver function...
October 19, 2018: Clinical Hemorheology and Microcirculation
Sarah Kammerer, Jan-Heiner Küpper
BACKGROUND: Drug induced liver injury (DILI) is the most frequent cause for failure of new drugs in clinical studies. Thus, toxicity studies are indispensable during drug development. The proliferative human liver cell strain HepaFH3 with promising primary-like cellular properties might be a suitable liver model for such studies, but their cytochrome-P450 (CYP) expression is still in low ranges compared to freshly isolated primary human hepatocytes. OBJECTIVE: We aimed to optimize the induction protocol for CYP1A2 and CYP3A4 in HepaFH3 to obtain a physiologically relevant in vitro liver model...
October 19, 2018: Clinical Hemorheology and Microcirculation
Juan Zhou, Hyewon Yang, Christian Lehmann
Sepsis is a medical condition caused by dysregulated systemic inflammatory response against infection, resulting in high mortality. Despite intensive research over the last few decades, the results from multiple clinical trials targeting specific inflammatory mediators have been disappointing. In the present study, we investigated the role of G protein-coupled receptor GPR55 modulation on immune response in an experimental sepsis model (endotoxemia). Immune response was evaluated by analyzing leukocyte-endothelial interactions and capillary perfusion in the intestinal microcirculation using intravital microscopy...
October 19, 2018: Clinical Hemorheology and Microcirculation
Sonia Salha, Sebastian Gehmert, Vanessa Brébant, Alexandra Anker, Markus Loibl, Lukas Prantl, Sanga Gehmert
INTRODUCTION: Mesenchymal stem cells (MSCs) have been described in breast cancer models to migrate towards carcinoma and integrate into tumor associated stroma supporting tumor growth, increasing their metastatic potency and contributing to tumor-angiogenesis. Platelet-derived growth factor (PDGF) isoforms (AA, BB, CC) stimulate growth, survival and motility of MSCs and certain other cell types. Noteworthy, breast carcinomas are known to express PDGF. We aim to further shed light on i) the relevance of the different PDGF isoforms on adipose tissue derived stem cells (ASCs) migration and ii) the underlying pathway dependent on PDGF stimulation...
October 19, 2018: Clinical Hemorheology and Microcirculation
Yan Nie, Weiwei Wang, Xun Xu, Jie Zou, Thanga Bhuvanesh, Burkhard Schulz, Nan Ma, Andreas Lendlein
Bioengineered cell substrates are a highly promising tool to govern the differentiation of stem cells in vitro and to modulate the cellular behavior in vivo. While this technology works fine for adult stem cells, the cultivation of human induced pluripotent stem cells (hiPSCs) is challenging as these cells typically show poor attachment on the bioengineered substrates, which among other effects causes substantial cell death. Thus, very limited types of surfaces have been demonstrated suitable for hiPSC cultures...
October 17, 2018: Clinical Hemorheology and Microcirculation
Nana-Yaw Bimpong-Buta, Peter Jirak, Bernhard Wernly, Michael Lichtenauer, Thorben Knost, Thaer Abusamrah, Malte Kelm, Christian Jung
BACKGROUND: Parabolic flights offer a unique platform for human experiments in short-term weightlessness. It is generally known that human organ systems react to changes of gravity. Yet, little is known about alterations of blood parameters under these conditions with special emphasis on blood rheology. OBJECTIVE: We investigated the alterations of distinct blood parameters after exposure to weightlessness. METHODS: 14 healthy volunteers underwent short-term phases of weightlessness induced by parabolic flight...
October 17, 2018: Clinical Hemorheology and Microcirculation
Janis L Vahldiek, Christoph Erxleben, Keno Kyrill Bressem, Ole Gemeinhardt, Franz Poch, Bernhard Hiebl, Kai S Lehmann, B Hamm, Stefan M Niehues
BACKGROUND: Radiofrequency ablation (RFA) is an important treatment option for hepatic tumors and metastases. Post-ablation recurrence rates are reported up to 36.5 percent and seem to depend on tumor size, intrahepatic localization of tumors and adjacent hepatic vessels. Multipolar RFA has the potential to overcome/reduce these limitations. Experimental and standardized data on achievable lesion sizes, influence of hepatic vessels and non-invasive evaluation of complete ablation is still insufficient...
October 17, 2018: Clinical Hemorheology and Microcirculation
Pamela Zengel, Florian Notter, Dirk A Clevert
BACKGROUND: Ultrasound is the method of choice for preoperative evaluation of masses of the parotid glands. However, existing methods do not allow for definite differentiation between the most common benign and malignant tumors. OBJECTIVE: Thus, we evaluated the benefits of Virtual Touch Quantification (VTQ) and Virtual Touch imaging quantification (VTIQ) for improving preoperative evaluation of parotid tumors. METHODS: We investigated eight lymph nodes and 41 tumors of the parotid gland via ultrasound, color Doppler ultrasound, VTIQ and VTQ shear wave imaging...
October 16, 2018: Clinical Hemorheology and Microcirculation
T Aung, C Taeger, S Geis, D Schiltz, E Brix, C Wenzel, P Lamby, A Kehrer, L Prantl, V Brebant
INTRODUCTION: Supermicrosurgical lymphaticovenular anastomosis (LVA) toward becoming a treatment alternative for treatment and for surgical management of refractory lymphedema. Effective LVA requires supermicrosurgical systems to detect and anastomose lymphatic vessels, as they have a small vessel gauge measuring less than 0.5 mm. METHOD: The antro- and retrograd deep LVAs were performed with the combination of superior-edge-of-the-knee incision method and ventral ankle joint incision method...
October 15, 2018: Clinical Hemorheology and Microcirculation
V Schwarze, K Mueller-Peltzer, G Negrão de Figueiredo, F Lindner, J Rübenthaler, D-A Clevert
BACKGROUND: Hepatic echinococcosis (HE) is a zoonosis and depicts a rare but potentially lethal disease caused by larval infestation of E. multilocularis (alveolar echinococcosis, AE) and E. granulosus (cystic echinocococcosis, CE). In many countries, HE is a critical public health problem. Clinically, HE patients initially are often asymptomatic for years. Depending on the echinococcal manifestations patients can later develop unspecific symptoms as fatigue, abdominal pain and may present with elevated transaminases, jaundice and hepatomegaly...
October 15, 2018: Clinical Hemorheology and Microcirculation
T Aung, P I Heidekrueger, S Geis, F Von Kunow, C Taeger, C Strauss, C Wendl, V Brebant, L Prantl, A Hillmann
No abstract text is available yet for this article.
October 15, 2018: Clinical Hemorheology and Microcirculation
B Schlenker, M Apfelbeck, A Buchner, C Stief, D-A Clevert
INTRODUCTION: Prostate cancer (PCa) is one of the most common malignancies in men. The diagnostic standard to confirm prostate cancer is the transrectal ultrasound-guided biopsy. However, this procedure is associated with the underdetection of clinically significant prostate cancer and therefore needs to be improved. In the last years MRI fusion based targeted biopsy gained importance as consequence. In this study, we evaluated the quality of MRI ultrasound image fusion and evaluated factors influencing the image fusion quality...
October 15, 2018: Clinical Hemorheology and Microcirculation
Natascha Platz Batista da Silva, Ernst Michael Jung, Friedrich Jung, Hans J Schlitt, Matthias Hornung
AIM: Aim of this study was to firstly describe reproducible, objective perfusion parameters of contrast-enhanced ultrasound (CEUS) kinetics of parathyroid gland adenoma (PA) using perfusion analysis software (VueBox®, Bracco, Italy). Thereby the efficiency of quantitative CEUS for characterization of PA should be evaluated comparing US to postoperative histopathological findings after PA resection. MATERIAL AND METHODS: 42 patients with symptoms/lab work suggestive of pHPT presented a parathyroid gland lesion in B-mode US, which was consequently analyzed by dynamic CEUS...
October 15, 2018: Clinical Hemorheology and Microcirculation
M Ahmed, S Lutze, M Tembulatow, G Daeschlein, M Jünger, A Arnold
BACKGROUND: Chronic venous disease (CVD) is extremely common worldwide. The prevalence is increasing with age and is associated with a reduced quality of life, particularly in relation to pain, physical function and mobility. Symptomatic chronic venous insufficiency (CVI) with venous ulcer at its' endpoint, indicates interventional surgery to cure venous reflux therewith promoting wound healing and preventing recurrence. To our knowledge up to now no coherent study has been undertaken in patients with CVI who underwent an open venous surgery concerning complications, venous hemodynamics and perioperative symptomology in one study population...
October 11, 2018: Clinical Hemorheology and Microcirculation
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