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Parasitology International

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https://www.readbyqxmd.com/read/28193534/implementation-of-new-tools-in-molecular-epidemiology-studies-of-echinococcus-granulosus-sensu-lato-in-south-america
#1
Héctor G Avila, Guilherme B Santos, Marcela A Cucher, Natalia Macchiaroli, Matías G Pérez, Germán Baldi, Oscar Jensen, Verónica Pérez, Raúl López, Perla Negro, Exequiel Scialfa, Arnaldo Zaha, Henrique B Ferreira, Mara Rosenzvit, Laura Kamenetzky
The aim of this work was to determine Echinococcus granulosus sensu lato species and genotypes in intermediate and definitive hosts and in human isolates from endemic regions of Argentina and Brazil including those where no molecular data is available by a combination of classical and alternative molecular tools. A total of 227 samples were isolated from humans, natural intermediate and definitive hosts. Amplification of cytochrome c oxidase subunit I gene fragment was performed and a combination of AluI digestion assay, High Resolution Melting analysis (HRM) assay and DNA sequencing was implemented for Echinococcus species/genotype determination...
February 11, 2017: Parasitology International
https://www.readbyqxmd.com/read/28189767/morphological-and-molecular-identification-of-the-liver-fluke-opisthorchis-viverrini-in-the-first-intermediate-host-bithynia-snails-and-its-prevalence-in-kampong-cham-province-cambodia
#2
Dusit Boonmekam, Suluck Namchote, Hajime Matsuda, Masashi Kirinoki, Kazuko Miyamoto, Muth Sinuon, Duangduen Krailas
No abstract text is available yet for this article.
February 8, 2017: Parasitology International
https://www.readbyqxmd.com/read/28167154/pilot-field-trial-of-the-eg95-vaccine-against-ovine-cystic-echinococcosis-in-rio-negro-argentina-humoral-response-to-the-vaccine
#3
Edmundo Larrieu, Thelma Verónica Poggio, Guillermo Mujica, Charles G Gauci, Jose Luis Labanchi, Eduardo Herrero, Daniel Araya, Claudia Grizmado, Arnoldo Calabro, Gabriel Talmon, Pablo Crowley, Graciela Santillán, Katherina Vizcaychipi, Marcos Seleiman, Luis Sepulveda, Marcos Arezo, Mariela García Cachau, Roberto Lamberti, Leonardo Molina, Lilia Gino, Meritxell Donadeu, Marshall W Lightowlers
No abstract text is available yet for this article.
February 4, 2017: Parasitology International
https://www.readbyqxmd.com/read/28163250/pampas-fox-lycalopex-gymnocercus-new-intermediate-host-of-sarcocystis-svanai-apicomplexa-sarcocystidae
#4
Nathalia P Scioscia, Leandro Olmos, Antonella Gorosábel, Lucía Bernad, Julieta Pedrana, Yanina P Hecker, Ignacio Gual, M Laura Gos, Guillermo M Denegri, Dadín P Moore, Gastón Moré
Several Sarcocystis spp. have carnivores as definitive host and sarcocysts are common in muscles of herbivores (intermediate host). However, sarcocysts have been found in muscles of wild and domestic carnivores suggesting they are intermediate host for some Sarcocystis spp. Here, we report mature sarcocysts in the muscles of Pampas fox (Lycalopex gymnocercus). A total of 36 free-living foxes were analyzed. Different skeletal muscles were assessed by microscopic and molecular methods. Cysts and/or DNA of Sarcocystis sp...
February 3, 2017: Parasitology International
https://www.readbyqxmd.com/read/28163249/activation-and-exhaustion-of-antigen-specific-cd8-t-cells-occur-in-different-splenic-compartments-during-infection-with-plasmodium-berghei
#5
Ganchimeg Bayarsaikhan, Mana Miyakoda, Kazuo Yamamoto, Daisuke Kimura, Masoud Akbari, Masao Yuda, Katsuyuki Yui
The spleen is the major organ in which T cells are primed during infection with malaria parasites. However, little is known regarding the dynamics of the immune responses and their localization within the splenic tissue during malaria infection. We examined murine CD8(+) T cell responses during infection with Plasmodium berghei using recombinant parasites expressing a model antigen ovalbumin (OVA) protein and compared the responses with those elicited by Listeria monocytogenes expressing the same antigen. OVA-specific CD8(+) T cells were mainly activated in the white pulp of the spleen during malaria infection, as similarly observed during Listeria infection...
February 3, 2017: Parasitology International
https://www.readbyqxmd.com/read/28137668/praobdellidae-hirudinida-arhynchobdellida-is-not-specific-only-to-the-mucous-membrane-after-all-discovery-of-a-praobdellid-leech-feeding-on-the-japanese-freshwater-crab-geothelphusa-dehaani
#6
Takafumi Nakano, Ko Tomikawa, Takahiro Sakono, Natsuhiko Yoshikawa
Praobdellid leech species have been known to infest vertebrate mucous-membrane; some of them have been assumed to be invertebrate bloodsuckers. Praobdellid individuals were found feeding on the Japanese freshwater crab, Geothelphusa dehaani, at Mt. Tsukuba, Ibaraki Prefecture, Japan. The leech had inserted its head into the intersegmental membrane between the crab's carapace and legs. Our findings represent a first invertebrate host record for praobdellid leeches. Additionally, molecular phylogenetic trees based on nuclear 18S and 28S rRNA, mitochondrial cytochrome c oxidase subunit I, and 12S rRNA sequences as well as the leech morphological characteristics showed that the present leech might belong to an unrecognized praobdellid lineage: a taxonomic revision of the Japanese praobdellid species is needed...
January 28, 2017: Parasitology International
https://www.readbyqxmd.com/read/28137670/effects-of-thai-piperaceae-plant-extracts-on-neospora-caninum-infection
#7
Arpron Leesombun, Sookruetai Boonmasawai, Yoshifumi Nishikawa
Neosporosis has a worldwide distribution and causes economic losses in farming, particularly by increasing the risk of abortion in cattle. This study investigated the effects of Thai piperaceae (Piper betle, P. nigrum, and P. sarmentosum) extracts on Neospora caninum infections in vitro and in vivo. In an in vitro parasite growth assay based on the green fluorescent protein (GFP) signal, P. betle was the most effective extract at inhibiting parasite growth in human foreskin fibroblast cells (IC50 of GFP-expressing N...
January 27, 2017: Parasitology International
https://www.readbyqxmd.com/read/28137669/effective-gene-delivery-to-trypanosoma-cruzi-epimastigotes-through-nucleofection
#8
Lisandro Pacheco-Lugo, Yirys Díaz-Olmos, José Sáenz-García, Christian Macagnan Probst, Wanderson Duarte DaRocha
New opportunities have raised to study the gene function approaches of Trypanosoma cruzi after its genome sequencing in 2005. Functional genomic approaches in Trypanosoma cruzi are challenging due to the reduced tools available for genetic manipulation, as well as to the reduced efficiency of the transient transfection conducted through conventional methods. The Amaxa nucleofector device was systematically tested in the present study in order to improve the electroporation conditions in the epimastigote forms of T...
January 27, 2017: Parasitology International
https://www.readbyqxmd.com/read/28132801/high-pathogenicity-and-strong-immunogenicity-of-a-chinese-isolate-of-eimeria-magna-p%C3%A3-rard-1925
#9
Geru Tao, Yunzhou Wang, Chao Li, Xiaolong Gu, Ping Cui, Sufang Fang, Xun Suo, Xianyong Liu
Coccidia infection of rabbits with one or several species of parasites of the genus Eimeria causes coccidiosis, a disease leading to huge economic losses in the rabbit industry. Eimeria magna, one of the causal agents of rabbit coccidiosis, was characterized as mildly pathogenic and moderately immunogenic in previous studies. In this study, we identified a Chinese isolate of E. magna by testing its biological features (oocyst morphology and size, prepatent time) and sequencing its internal transcribed spacer 1 (ITS-1) DNA fragment...
January 27, 2017: Parasitology International
https://www.readbyqxmd.com/read/28131768/brachylaima-ezohelicis-sp-nov-trematoda-brachylaimidae-found-from-the-land-snail-ezohelix-gainesi-with-a-note-of-an-unidentified-brachylaima-species-in-hokkaido-japan
#10
Minoru Nakao, Tsukasa Waki, Mizuki Sasaki, Jason L Anders, Daisuke Koga, Mitsuhiko Asakawa
In the Japanese Archipelago, Ezohelix gainesi, a member of bradybaenid land snails, is endemic mainly to the island of Hokkaido. During July to August of 2016, a survey to detect trematode infections from E. gainesi was carried out at a forest city park in Asahikawa, Hokkaido. Systemic infections of the snails with sporocysts containing short-tailed cercariae were found in 5.3% of 94 individuals examined. Furthermore, most of them (90.4%) harbored non-encysted metacercariae within their kidneys. A DNA sequence identification revealed that both of the sporocyst and the metacercaria belong to an unknown species of the family Brachylaimidae...
January 25, 2017: Parasitology International
https://www.readbyqxmd.com/read/28110081/isolation-and-partial-characterization-of-an-immunogenic-antigen-of-giardia-lamblia
#11
Jael Quintero, Alejandra Valdez, Brenda Samaniego, Gloria Lopez-Romero, Humberto Astiazaran-Garcia, Lucila Rascon, Linda Breci, Adriana Garibay-Escobar, Ramón Robles-Zepeda, Carlos Velazquez
Humoral and cellular immune responses play an important role during Giardia lamblia infection. Several Giardia proteins have been identified as immunogenic antigens based on their elicited humoral immune response. Poorly is known about Giardia antigens that stimulate a cellular immune response. The main purpose of this study was to isolate and partial characterize an immunogenic antigen (5G8) of G. lamblia. The 5G8 protein was isolated from G. lamblia trophozoite lysates by affinity chromatography using moAb 5G8-coupled CNBr-Sepharose...
January 18, 2017: Parasitology International
https://www.readbyqxmd.com/read/28069406/message-from-dean-of-faculty-of-medicine-khon-kaen-university
#12
EDITORIAL
Charnchai Panthongviriyakul
No abstract text is available yet for this article.
January 7, 2017: Parasitology International
https://www.readbyqxmd.com/read/28069405/message-from-permanent-secretary-of-public-health
#13
EDITORIAL
Sopon Mekthon
No abstract text is available yet for this article.
January 7, 2017: Parasitology International
https://www.readbyqxmd.com/read/28069407/a-historical-review-of-small-liver-fluke-infections-in-humans
#14
EDITORIAL
Banchob Sripa, Smarn Tesana, Nataliya Yurlova, Yukifumi Nawa
No abstract text is available yet for this article.
January 6, 2017: Parasitology International
https://www.readbyqxmd.com/read/27965165/suspected-cases-of-cholangiocarcinoma-seen-in-reference-hospitals-in-lao-people-s-democratic-republic
#15
Phonepasong Ayé Soukhathammavong, Youthanavanh Vonghachack, Christoph Hatz, Kongsap Akkhavong, Peter Odermatt
Cholangiocarcinoma (CCA) poses a significant public health problem in Southeast Asia, with chronic Opisthorchis viverrini infection being the main risk factor. Epidemiological data on CCA is not available for Lao People Democratic Republic (Lao PDR), though there is a high prevalence of O. viverrini infection in the country. A hospital record-based retrospective study was carried out in six referral hospitals (four in the capital city, one in Savannakhet, one in Pakse) to assess the number of suspected CCA cases admitted between January 2006 and December 2010...
December 11, 2016: Parasitology International
https://www.readbyqxmd.com/read/27956092/ultrasonographic-features-of-hepatobiliary-in-opisthorchiasis
#16
Eimorn Mairiang
Infection with the liver fluke, Opisthorchis viverrini (OV), is an important public health problem in northeast Thailand where people have a habit of eating raw or inadequately cooked fish. Opisthorchiasis Is associated with a number of asymptomatic hepatobiliary abnormalities and cholangiocarcinoma (CCA) that can be observed conveniently by ultrasonography. The ultrasonographicclassification of hepatobiliary pathology opisthorchiasis will be presented.
December 10, 2016: Parasitology International
https://www.readbyqxmd.com/read/27939972/opisthorchiasis-in-western-siberia-epidemiology-and-distribution-in-human-fish-snail-and-animal-populations
#17
Natalia I Yurlova, Elena N Yadrenkina, Natalia M Rastyazhenko, Elena А Serbina, Viktor V Glupov
Opisthorchiasis is a widespread helminth infection in Russia. The largest opisthorchiasis endemic focus in the world is the Ob river watershed in Western Siberia. The main causative agent of this condition is the liver fluke, Opisthorchis felineus. In addition, another liver fluke species in the Opisthorchiidae family, Metorchis bilis, causes a symptomatically similar disease, metorchiasis. Despite a long research history going back to 1927, opisthorchiasis remains a serious problem in Russia, and numerous questions related to the epidemiology of these liver fluke infections and their patterns of distribution in Western Siberia, the causes of high prevalence in different populations, and the prognosis of the epidemiological situation remain to be answered...
December 8, 2016: Parasitology International
https://www.readbyqxmd.com/read/27919744/co-infections-with-liver-fluke-and-helicobacter-species-a-paradigm-change-in-pathogenesis-of-opisthorchiasis-and-cholangiocarcinoma
#18
REVIEW
Banchob Sripa, Raksawan Deenonpoe, Paul J Brindley
Infection with the fish-borne liver fluke Opisthorchis viverrini is classified by the International Agency for Research on Cancer as a Group 1 carcinogen: definitely carcinogenic in humans. Cofactors likely contribute to bile duct cancer (cholangiocarcinoma) caused by this infection. Here we review recent findings that address the role of liver fluke associated H. pylori in hepatobiliary disease and malignancy. We hypothesize that co-infection by O. viverrini and the bacillus Helicobacter pylori is central of liver fluke infection associated cholangiocarcinoma...
December 3, 2016: Parasitology International
https://www.readbyqxmd.com/read/28115231/molecular-detection-and-species-identification-of-enterocytozoon-bieneusi-isolated-from-immunocompetent-orang-asli-in-malaysia
#19
Azah Ashikin, Hesham M Al-Mekhlafi, Norhayati Moktar, Tengku Shahrul Anuar
Most studies of opportunistic infections focus on immunocompromised patients. However, there is a lack of information on microsporidiosis in healthy people (immunocompetent) worldwide. This study aimed to detect and identify microsporidia species in immunocompetent Orang Asli living in Pahang, Malaysia. Orang Asli is a collective term for a group of indigenous people that usually reside in the interior regions of Peninsular Malaysia. They comprise about 0.7% of the total population in Malaysia and 76% of them lived below the poverty line i...
April 2017: Parasitology International
https://www.readbyqxmd.com/read/28110082/acute-larvicidal-toxicity-of-five-essential-oils-pinus-nigra-hyssopus-officinalis-satureja-montana-aloysia-citrodora-and-pelargonium-graveolens-against-the-filariasis-vector-culex-quinquefasciatus-synergistic-and-antagonistic-effects
#20
Giovanni Benelli, Roman Pavela, Angelo Canale, Kevin Cianfaglione, Giampiero Ciaschetti, Fabio Conti, Marcello Nicoletti, Sengottayan Senthil-Nathan, Heinz Mehlhorn, Filippo Maggi
Mosquito vector control is facing a number of important and timely challenges, mainly due to the rapid development of pesticide resistance and environmental concerns. In this scenario, screening of botanical resources for their mosquitocidal activity may offer effective and eco-friendly tools against Culicidae vectors. Culex quinquefasciatus Say (Diptera: Culicidae) is a vector of lymphatic filariasis and of dangerous arboviral diseases, such as West Nile and St. Louis encephalitis. In this study, the chemical composition of five essential oils obtained from different plants, namely Pinus nigra J...
April 2017: Parasitology International
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