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Parasitology International

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https://www.readbyqxmd.com/read/28629829/morphological-variability-and-molecular-characterization-of-pomphorhynchus-zhoushanensis-sp-nov-acanthocephala-pomphorhynchidae-with-comments-on-the-systematic-status-of-pomphorhynchus-monticelli-1905
#1
Liang Li, Hui-Xia Chen, Omar M Amin, Yue Yang
Species of Pomphorhynchus Monticelli, 1905 commonly parasitize the digestive tract of freshwater fishes, and rarely occur in marine fishes and amphibians. In the present study, Pomphorhynchus zhoushanensis sp. nov., collected from the barred knifejaw Oplegnathus fasciatus (Temminck & Schlegel) (Perciformes: Oplegnathidae) in the East China Sea, was described using integrated approaches, including light and scanning electron microscopy, and the sequencing and analysing of ribosomal [small ribosomal DNA (18S) and internal transcribed spacer (ITS)] and mitochondrial [cytochrome c oxidase subunit 1 (cox1)] target regions...
June 16, 2017: Parasitology International
https://www.readbyqxmd.com/read/28624295/molecular-characterization-of-sarcocystis-spp-in-intestine-mucosal-scrapings-and-fecal-samples-of-pampas-fox-lycalopex-gymnocercus
#2
Nathalia Paula Scioscia, María Laura Gos, Guillermo María Denegri, Gastón Moré
Sarcocystis spp. are obligatory intracellular protozoan parasites which can infect humans and animals. Most of Sarcocystis species were identified based on the detection of muscle cysts in different intermediate hosts (IH). Regarding to natural infection in definitive host, there are few reports which have reached to determining species of Sarcocystis. The present work was aimed to studying the occurrence of Sarcocystis spp. (oocysts and sporocysts) in mucosal scrapings of small intestine and fecal samples of one the most abundant wild canids from South America, Lycalopex gymnocercus (Pampas fox), and to identify the Sarcocystis spp...
June 14, 2017: Parasitology International
https://www.readbyqxmd.com/read/28602862/sag5b-and-sag5c-combined-vaccine-protects-mice-against-toxoplasma-gondii-infection
#3
Gang Lu, Jian Zhou, Aihua Zhou, Yali Han, Jingjing Guo, Pengxia Song, Huaiyu Zhou, Hua Cong, Ming Hou, Lin Wang, Shenyi He
Infections with the protozoan parasite Toxoplasma gondii, which are common around the world, can lead to congenital infections in humans. T. gondii surface antigen protein 5B (SAG5B) and SAG5C are potential stimulators of humoral and cellular immune responses. In this study, a multi-antigenic DNA vaccine constructed to express T. gondii SAG5B and SAG5C proteins simultaneously was used to immunize BALB/c mice to evaluate the protective efficacy of the vaccine. IgG antibody and gamma interferon (IFN-γ) cytokine production in the pSAG5B/SAG5C DNA vaccine group were significantly higher (0...
June 8, 2017: Parasitology International
https://www.readbyqxmd.com/read/28602861/qpcr-for-the-detection-of-foodborne-trypanosoma-cruzi
#4
Poliana Alves de Souza Godoi, Claudio Adriano Piechnik, Ana Caroline de Oliveira, Michelle Zibetti Sfeir, Emanuel Maltempi de Souza, Hervé Rogez, Vanete Thomaz Soccol
Here we presented a potential real-time PCR (qPCR) method with public health importance and relevance for detection of Trypanosoma cruzi in açai pulp. There is not a current process to identify T. cruzi in açai, that ensures innocuity of this food concerning oral transmission. First, six new primers were designed using the DNA sequences of T. cruzi y152 and Emerald strains obtained from GenBank. For primers evaluation and titration they were validated regarding the amplification and not with the fluorophore chosen 1ngμL(-1) of the T...
June 8, 2017: Parasitology International
https://www.readbyqxmd.com/read/28571766/evaluation-of-novel-oocyst-wall-protein-candidates-of-toxoplasma-gondii
#5
Doaa Salman, Liria Hiromi Okuda, Akio Ueno, George Dautu, Feifei Zhang, Makoto Igarashi
Oocyst stage of Toxoplasma gondii is characterized by a durable wall that confers a strong protection to this protozoan parasite in face of harsh environmental conditions. Thus, it is considered the key for transmission of T. gondii. Analysis of oocyst wall composition is mandatory therefore; the aim of this study was to identify novel T. gondii oocyst wall proteins and test their use in detection of these oocysts in environmental samples. Five candidates of novel T. gondii oocyst wall proteins (TgOWPs) were identified and named TgOWP8 through TgOWP12...
May 29, 2017: Parasitology International
https://www.readbyqxmd.com/read/28571765/potential-of-synthetic-endoperoxides-against-trichomonas-vaginalis-in-vitro
#6
Min-Young Seo, Jae-Sook Ryu, Akira Sato, Yuji Kurosaki, Kyung Soo Chang, Hye-Sook Kim
Metronidazole is well known for medicine against Trichomonas vaginalis infection, but it has side effects though it is effective, and especially because reports of metronidazole-tolerant species are increasing, the development of new medicine is being required. Here, we noticed the killing effects of endoperoxide compounds, N-89 and N-251 as new antimalarial drug candidates, on T. vaginalis and searched the possibility of development of new medicine. We added each of metronidazole, artemisinin, and two of new endoperoxides (N-89 and N-251) to metronidazole-resistant and -sensitive species and compared its anti-trichomonal efficacy...
May 29, 2017: Parasitology International
https://www.readbyqxmd.com/read/28552633/early-and-late-gene-expression-profiles-of-the-ovine-mucosa-in-response-to-haemonchus-contortus-infection-employing-illumina-rna-seq-technology
#7
Saeed El-Ashram, Ibrahim Al Nasr
We conducted herein transcriptome sequencing of the ovine abomasal tissues using the Illumina HiSeq 4000 platform to segregate early and late H. contortus-infected sheep (7 and 50days post-infected groups, respectively) from the control naive ones. A total of 548, 357 and 7 were substantially induced genes in 7days post-infection versus uninfected-control group, 50days post-infection versus 7days post-infection (7dpi), and 50days post-infection (7dpi) versus uninfected-control group, respectively. However, a total of 301, 355 and 11 were significantly repressed genes between 7dpi versus uninfected-control group, 50dpi versus 7dpi, and 50dpi versus uninfected-control group, correspondingly...
May 24, 2017: Parasitology International
https://www.readbyqxmd.com/read/28527785/the-effectiveness-of-a-fixed-dose-combination-pour-on-formulation-of-1-25-fipronil-and-2-5-fluazuron-against-economically-important-ectoparasites-and-associated-pharmacokinetics-in-cattle
#8
Welber Daniel Zanetti Lopes, Rafael Marin Chiummo, Luis Fernando Vettorato, Daniel de Castro Rodrigues, Rafael Bazaglia Sonada
The present work consisted of eight studies to evaluate the ectoparasiticidal spectrum and determine the pharmacokinetic parameters of a pour-on combination of fipronil 1.25mg/kg+fluazuron 2.5mg/kg for cattle against Rhipicephalus microplus, Haematobia irritans and the larvae of Dermatobia hominis and Cochliomyia hominivorax. The analysis fipronil and fluazuron were performed by liquid chromatography using a mass detector for the detection and quantification of analytes (LC-MS/MS). Additionally, in two of these studies, the animals were artificially infested with R...
May 17, 2017: Parasitology International
https://www.readbyqxmd.com/read/28526420/responses-to-comments-in-a-letter-to-the-editor-parint_2017_67-anonymized
#9
EDITORIAL
Yasushi Kawakami
No abstract text is available yet for this article.
May 16, 2017: Parasitology International
https://www.readbyqxmd.com/read/28501634/helminth-infracommunity-of-the-cane-toad-rhinella-marina-anura-bufonidae-within-its-native-distribution-range
#10
Nallely Ruiz-Torres, Luis García-Prieto, Omar Lagunas-Calvo, Juan Violante-González, David Osorio Sarabia
During August 2010, a total of 66 cane toad Rhinella marina were collected in Laguna de Coyuca, Guerrero, Mexico and examined for the presence of helminths. Ninety-two percent were parasitized by 1 or more parasite species. A total of 4624 individuals were found, belonging to 8 helminth taxa (1 digenean, 1 cestode and 6 nematodes). Infracommunities harbored by this host species are highly variable (mean Jaccard similarity index 0.31±0.27); mean percent similarity index 0.26±0.32) and depauperate (mean richness 1...
May 10, 2017: Parasitology International
https://www.readbyqxmd.com/read/28499980/morphological-and-molecular-identification-of-sarcocystis-arctica-sarcocysts-in-three-red-foxes-vulpes-vulpes-from-the-czech-republic
#11
Ivan Pavlásek, Ondřej Máca
Muscular sarcocystosis by Sarcocystis arctica was found for the first time in the Czech Republic, in different muscles of red fox (Vulpes vulpes). Cysts were slim, elongated, thread-like, whitish, 1-7mm long, and 206-270μm wide; bradyzoites were 7.9×2.7μm in unstained wet mounts and 9.2×2.9μm in cyst Giemsa-stained smears. The cyst wall was thin, with short villi-like protrusions, and no host response was observed in the histological sections. Examination of the distribution and intensity of sarcocysts in 17 different muscle groups revealed that the highest intensity was in the cranial tibial muscle (>15 cysts in compressoria), followed by the diaphragm, forearm, and other groups (with intensities of 3-15 cysts in compressoria)...
May 9, 2017: Parasitology International
https://www.readbyqxmd.com/read/28495225/detection-and-molecular-status-of-isospora-sp-from-the-domestic-pigeon-columba-livia-domestica
#12
Ryuma Matsubara, Yasuhiro Fukuda, Fumi Murakoshi, Osamu Nomura, Toru Suzuki, Chika Tada, Yutaka Nakai
The domestic pigeon, Columba livia domestica, is reared for meat production, as a pet, or for racing. Few reports have characterized the parasitic protists from the genus Isospora isolated from Columbiformes. We detected Isospora-like oocysts from C. livia reared for racing. The oocyst contained two sporocysts, and each sporocyst included four sporozoites. The sporulated oocysts (n=4) were spherical; their mean diameters were 25.6 (24.0-27.2)×24.7 (23.4-26.0) μm. Micropyles, polar granules, and oocyst residuum were absent...
May 8, 2017: Parasitology International
https://www.readbyqxmd.com/read/28483708/gamasoidosis-by-the-special-lineage-l1-of-dermanyssus-gallinae-acarina-dermanyssidae-a-case-of-heavy-infestation-in-a-public-place-in-italy
#13
Marco Pezzi, Marilena Leis, Milvia Chicca, Lise Roy
Among Gamasina (Acari: Mesostigmata) mites, some dermanyssoid species are known to cause gamasoidosis, a human dermatitis characterized by papulosquamous eruptions and urticarian lesions. We describe a case of mite infestation which occurred in public conference halls in Ferrara (Italy), affecting four people who attended the place and showed signs of gamasoidosis. The mites were collected and characterized using scanning electron microscopy, light microscopy and mitochondrial DNA sequencing (Cytochrome c oxidase subunit I partial CDS)...
May 5, 2017: Parasitology International
https://www.readbyqxmd.com/read/28476340/first-report-of-human-intestinal-sarcocystosis-in-cambodia
#14
Virak Khieu, Hanspeter Marti, Saomony Chhay, Meng Chuor Char, Sinuon Muth, Peter Odermatt
Human intestinal sarcocystosis (HIS), caused by Sarcocystis species, is acquired by eating undercooked meat from sarcocyst-containing cattle (S. hominis, S. heydorni) and pigs (S. suihominis). We report on the detection of human intestinal Sarcocystis infections in a cross-sectional survey of Strongyloides stercoralis in early 2014, in Rovieng District, Preah Vihear Province, northern Cambodia. Among 1081 participants, 108 (10.0%) were diagnosed with Sarcocystis spp. oocysts in stool samples. Males had a significantly higher risk of infection than females (OR: 1...
May 2, 2017: Parasitology International
https://www.readbyqxmd.com/read/28465107/seroprevalence-of-toxoplasma-gondii-in-humans-and-pigs-in-north-sulawesi-indonesia
#15
Josef Tuda, Sri Adiani, Madoka Ichikawa-Seki, Kousuke Umeda, Yoshifumi Nishikawa
Toxoplasma gondii, an intracellular protozoan parasite, is a major public health concern throughout the world. Importantly, toxoplasmosis has several adverse effects, including neurological and ocular diseases. There are currently no data on the prevalence of T. gondii infection in humans or animals in North Sulawesi, although Indonesia is known to have a high seroprevalence of this parasite. In this study, the prevalence of T. gondii was determined in samples of humans and pigs from North Sulawesi using the latex agglutination test...
April 29, 2017: Parasitology International
https://www.readbyqxmd.com/read/28456494/phosphoinositide-binding-profiles-of-the-px-domains-of-giardia-lamblia
#16
Ananya Jana, Abhishek Sinha, Srimonti Sarkar
The membrane trafficking machinery that functions at the endomembrane system of Giardia lamblia appears to be significantly different from that present in most model eukaryotes. This machinery is important for encystation as cyst wall material is trafficked to the cell surface via encystation-specific vesicles. Since proteins containing the phosphoinositide-binding PX domains are known regulators of vesicular trafficking, BLAST search was used to identify the PX domains of G. lamblia. Six putative PX domain-containing ORFs were identified...
April 26, 2017: Parasitology International
https://www.readbyqxmd.com/read/28449979/morphological-molecular-and-phylogenetic-analyses-of-diplotriaena-bargusinica-skrjabin-1917-nematoda-diplotriaenidae
#17
Thainá Dutra Vieira, Marcia Raquel Pegoraro de Macedo, Fabiana Fedatto Bernardon, Gertrud Müller
The nematode Diplotriaena bargusinica is a bird air sac parasite, and its taxonomy is based mainly on morphological and morphometric characteristics. Increasing knowledge of genetic information variability has spurred the use of DNA markers in conjunction with morphological data for inferring phylogenetic relationships in different taxa. Considering the potential of molecular biology in taxonomy, this study presents the morphological and molecular characterization of D. bargusinica, and establishes the phylogenetic position of the nematode in Spirurina...
April 24, 2017: Parasitology International
https://www.readbyqxmd.com/read/28445773/taxonomic-comments-on-diphyllobothrium-hottai-yazaki-fukumoto-abe-1988-cestoda-diphyllobothriidae
#18
EDITORIAL
Minoru Nakao, Tetsuya Yanagida
No abstract text is available yet for this article.
April 23, 2017: Parasitology International
https://www.readbyqxmd.com/read/28435029/activity-of-carboxylesterases-glutathione-s-transferase-and-monooxygenase-on-rhipicephalus-microplus-exposed-to-fluazuron
#19
Fabrício Nascimento Gaudêncio, Guilherme Marcondes Klafke, Vinícius Menezes Tunholi-Alves, Thaís Paes Ferreira, Cristiane Nunes Coelho, Adivaldo Henrique da Fonseca, Isabele da Costa Angelo, Jairo Pinheiro
The objective of this study was to assess the effect of the exposure to fluazuron on the activity of common pesticide detoxification enzyme groups in the cattle tick (Rhipicephalus microplus). Engorged females of a susceptible strain (POA) and a resistant strain (Jaguar) were exposed in vitro to fluazuron and their eggs and larvae were used to compare the activities of the general esterases, mixed-function oxidases (MFO) and glutathione-S-transferase (GST). The results showed significant elevation in MFO contents and esterases activity in the resistant strain when compared with the susceptible strain, in eggs and larvae respectively...
April 20, 2017: Parasitology International
https://www.readbyqxmd.com/read/28408356/possible-antifibrotic-effect-of-gdc-0449-vismodegib-a-hedgehog-pathway-inhibitor-in-mice-model-of-schistosoma-induced-liver-fibrosis
#20
Abeer A Elhenawy, Rehab H Ashour, Nairmen Nabih, Naglaa M Shalaby, Nirmeen Megahed
Liver fibrosis is a pathological process complicating schistosomiasis. It is an active process of continuous extracellular matrix accumulation. In Egypt, schistosomiasis re-infection is a continuing problem especially in rural areas. In this study we examined the antifibrotic effect of GDC-0449 (Vismodegib), a hedgehog-pathway inhibitor as a new molecular target for Schistosoma-induced liver fibrosis, in addition to exploring its effect as antischistosomal drug. The effect of GDC-0449 alone or combined with Praziquantel was tried experimentally in infected mice with Schistosoma mansoni...
April 10, 2017: Parasitology International
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