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Parasitology International

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https://www.readbyqxmd.com/read/28288843/non-invasive-diagnosis-of-cutaneous-leishmaniasis-by-the-direct-boil-loop-mediated-isothermal-amplification-method-and-minion%C3%A2-nanopore-sequencing
#1
Kazuo Imai, Norihito Tarumoto, Kiyoko Amo, Makoto Takahashi, Naoya Sakamoto, Atsushi Kosaka, Yasuyuki Kato, Kei Mikita, Jun Sakai, Takashi Murakami, Yutaka Suzuki, Shigefumi Maesaki, Takuya Maeda
Cutaneous leishmaniasis (CL) is gaining attention as a public health problem. We present two cases of CL imported from Syria and Venezuela in Japan. We diagnosed them as CL non-invasively by the direct boil loop-mediated isothermal amplification method and an innovative sequencing method using the MinION™ sequencer. This report demonstrates that our procedure could be useful for the diagnosis of CL in both clinical and epidemiological settings.
March 11, 2017: Parasitology International
https://www.readbyqxmd.com/read/28263882/two-new-species-of-elopicola-digenea-aporocotylidae-from-hawaiian-ladyfish-elops-hawaiensis-eastern-sea-and-atlantic-tarpon-megalops-atlanticus-gulf-of-mexico-with-a-comment-on-monophyly-of-elopomorph-blood-flukes
#2
Raphael Orélis-Ribeiro, Kenneth M Halanych, Binh T Dang, Micah D Bakenhaster, Cova R Arias, Stephen A Bullard
Elopicola bristowi sp. n. infects the blood vascular system of Hawaiian ladyfish, Elops hawaiensis, in the Eastern Sea. It differs from the only nominal congener Elopicola nolancribbi by the combination of having rows of ventrolateral tegumental spines, a proportionally long oesophagus, anterior caeca, vasa efferentia coalescing ventral to the posterodextral margin of the testis, a post-testicular metraterm, a dextral common genital pore lateral to the oötype, and genitalia that are enantiomorphic relative to those of E...
March 3, 2017: Parasitology International
https://www.readbyqxmd.com/read/28263883/asymptomatic-plasmodium-malariae-infections-in-children-from-suburban-areas-of-yaound%C3%A3-cameroon
#3
Dongang Nana Rodrigue Roman, Ngono Ngane Annie Rosalie, Amit Kumar, Koanga Mogtomo Martin Luther, Vineeta Singh, Mouelle Sone Albert
The gold standard for malaria diagnosis is the microscopic examination of Giemsa stained thick blood smears though microscopy mostly may not detect the presence of Plasmodium species infections in asymptomatic samples. In the reported study, we used two diagnostic methods viz. the conventional microscopic examination and polymerase chain reaction (PCR) assay to analyse the asymptomatic malaria samples. PCR assay amplifying 18S small-subunit ribosomal RNA (SSU rRNA) gene of Plasmodium in 122 samples confirmed 68% of isolates as asymptomatic P...
March 2, 2017: Parasitology International
https://www.readbyqxmd.com/read/28257952/in-vitro-immunomodulatory-effects-of-microemulsions-with-levamisole-delivery-systems-on-blood-phagocytes-interacting-with-giardia-lamblia
#4
Mara Rosa Gil Hernandes, Lucélia Campelo Albuquerque Moraes, Elton Brito Ribeiro, Danny Laura Gomes Fagundes, Adenilda Cristina Honorio-França, Eduardo Luzía França
BACKGROUND: Giardiasis is one of the main parasites that infect the gastrointestinal tract of humans, affecting hundreds of millions of people worldwide, particularly in developing countries. Antiparasitics administered to treat giardiasis are inefficient in 20% of the cases, usually because of parasite resistance and side effects. In this scenario, microemulsions are a promising pharmaceutical alternative as carriers of molecules with therapeutic action that stimulate the immune system...
February 28, 2017: Parasitology International
https://www.readbyqxmd.com/read/28257951/gobioecetes-biwaensis-n-g-n-sp-monogenea-dactylogyridae-from-the-gills-of-a-freshwater-gobiid-fish-rhinogobius-sp-bw-takahashi-okazaki-2002-with-a-redescription-of-parancyrocephaloides-daicoci-yamaguti-1938
#5
Kazuo Ogawa, Naoki Itoh
A description is given of Gobioecetes biwaensis n. g., n. sp. (Monogenea: Dactylogyridae) on the gills of a freshwater gobiid fish, Rhinogobius sp. BW Takahashi & Okazaki, 2002 from Lake Biwa, Shiga Prefecture and Rhinogobius sp. from Sento Imperial Palace, Kyoto Prefecture, Japan. This monogenean is most similar to Parancyrocephaloides rhinogobius Ling, 1973, proposed here as Gobioecetes rhinogobius n. comb., collected from the gills of Rhinogobius giurinus (Rutter, 1897) in Hupei Province, China, but can be differentiated from it in that ventral hamuli of G...
February 28, 2017: Parasitology International
https://www.readbyqxmd.com/read/28257950/redescription-and-molecular-characterization-of-anoplocephala-manubriata-railliet-et-al-1914-cestoda-anoplocephalidae-from-a-sri-lankan-wild-elephant-elephas-maximus
#6
K U E Perera, Susiji Wickramasinghe, B V P Perera, K B A T Bandara, R P V J Rajapakse
The present work provides a detailed morphological and molecular description of Anoplocephala manubriata in elephants. Adult worms were recovered during an autopsy of a wild elephant in Elephant Transit Home, Udawalawe, Sri Lanka. Necropsy findings revealed a severe cestode infection in the small intestine. These tapeworms were tightly attached to the intestinal mucosae, resulted in hyperemic thickened intestinal mucosae, variable size irregular well-demarcated multifocal ulcerative regions sometimes covered with necrotic membranes and variable size, diffuse, well-demarcated raised nodular masses were evident in the small intestine...
February 28, 2017: Parasitology International
https://www.readbyqxmd.com/read/28232063/myxobolus-chushi-n-sp-myxozoa-myxosporea-parasitizing-schizothorax-niger-heckel-a-native-cyprinid-fish-from-wullar-lake-in-kashmir-himalayas
#7
Shoaib Ali Dar, Harpreet Kaur, M Z Chishti
In the study, a new species, Myxobolus chushi n. sp. infecting gills of wild specimens of Schizothorax niger (Heckel) inhabiting Wullar Lake in Kashmir Himalayas, (J&K) India has been described based on morphology of the myxospore and using partial 18S rDNA sequencing. Pathological changes in the gills have been studied with the help of histological sections stained with Luna's method. Twenty fish specimens were examined, out of which four had oval, white plasmodia in gills measuring 2.0×0.5mm. The myxospores were spherical to ovoidal in shape with slightly attenuated posterior end, measuring 11...
February 21, 2017: Parasitology International
https://www.readbyqxmd.com/read/28232044/clinical-and-pathological-effects-of-dirofilaria-repens-and-dirofilaria-immitis-in-a-dog-with-a-natural-co-infection
#8
Mircea Mircean, Angela Monica Ionică, Viorica Mircean, Adriana Györke, Andrei Răzvan Codea, Flaviu Alexandru Tăbăran, Marian Taulescu, Mirabela Oana Dumitrache
Canine dirofilarioses are mosquito-borne zoonotic diseases with a continuous expansion of their geographical distribution, as a consequence of different climatic and ecological factors. Dirofilaria immitis, the aetiological agent of heartworm disease, has gained a major veterinary interest, mainly due to its severe clinical implication. In the last decades, D. repens, despite of being regarded as a less pathogenic species, regained attention due to its recognized zoonotic potential. Romania has been traditionally regarded as a non-endemic country, but recent epidemiological surveys are highlighting the presence of both D...
February 13, 2017: Parasitology International
https://www.readbyqxmd.com/read/28232043/musculature-arrangement-and-locomotion-in-notocotylid-cercariae-digenea-notocotylidae-from-mud-snail-ecrobia-ventrosa-montagu-1803
#9
Darya Krupenko, Anna Gonchar
Cercariae of digenean family Notocotylidae are characterized by a set of morphological traits which make them easily distinguishable from any other. One of the key features is absence of ventral sucker. This affects basic ways of locomotion and attachment. To understand how these functions are fulfilled we studied musculature arrangement in cercariae of two species by means of fluorescent-phalloidin staining and confocal microscopy. We used Cercaria Notocotylidae sp. No. 11 and 12 Deblock, 1980 from mud snails Ecrobia (=Hydrobia) ventrosa...
February 13, 2017: Parasitology International
https://www.readbyqxmd.com/read/28193534/implementation-of-new-tools-in-molecular-epidemiology-studies-of-echinococcus-granulosus-sensu-lato-in-south-america
#10
Héctor G Avila, Guilherme B Santos, Marcela A Cucher, Natalia Macchiaroli, Matías G Pérez, Germán Baldi, Oscar Jensen, Verónica Pérez, Raúl López, Perla Negro, Exequiel Scialfa, Arnaldo Zaha, Henrique B Ferreira, Mara Rosenzvit, Laura Kamenetzky
The aim of this work was to determine Echinococcus granulosus sensu lato species and genotypes in intermediate and definitive hosts and in human isolates from endemic regions of Argentina and Brazil including those where no molecular data is available by a combination of classical and alternative molecular tools. A total of 227 samples were isolated from humans, natural intermediate and definitive hosts. Amplification of cytochrome c oxidase subunit I gene fragment was performed and a combination of AluI digestion assay, High Resolution Melting analysis (HRM) assay and DNA sequencing was implemented for Echinococcus species/genotype determination...
February 11, 2017: Parasitology International
https://www.readbyqxmd.com/read/28189767/morphological-and-molecular-identification-of-the-liver-fluke-opisthorchis-viverrini-in-the-first-intermediate-host-bithynia-snails-and-its-prevalence-in-kampong-cham-province-cambodia
#11
Dusit Boonmekam, Suluck Namchote, Hajime Matsuda, Masashi Kirinoki, Kazuko Miyamoto, Muth Sinuon, Duangduen Krailas
No abstract text is available yet for this article.
February 8, 2017: Parasitology International
https://www.readbyqxmd.com/read/28167154/pilot-field-trial-of-the-eg95-vaccine-against-ovine-cystic-echinococcosis-in-rio-negro-argentina-humoral-response-to-the-vaccine
#12
Edmundo Larrieu, Thelma Verónica Poggio, Guillermo Mujica, Charles G Gauci, Jose Luis Labanchi, Eduardo Herrero, Daniel Araya, Claudia Grizmado, Arnoldo Calabro, Gabriel Talmon, Pablo Crowley, Graciela Santillán, Katherina Vizcaychipi, Marcos Seleiman, Luis Sepulveda, Marcos Arezo, Mariela García Cachau, Roberto Lamberti, Leonardo Molina, Lilia Gino, Meritxell Donadeu, Marshall W Lightowlers
No abstract text is available yet for this article.
February 4, 2017: Parasitology International
https://www.readbyqxmd.com/read/28163250/pampas-fox-lycalopex-gymnocercus-new-intermediate-host-of-sarcocystis-svanai-apicomplexa-sarcocystidae
#13
Nathalia P Scioscia, Leandro Olmos, Antonella Gorosábel, Lucía Bernad, Julieta Pedrana, Yanina P Hecker, Ignacio Gual, M Laura Gos, Guillermo M Denegri, Dadín P Moore, Gastón Moré
Several Sarcocystis spp. have carnivores as definitive host and sarcocysts are common in muscles of herbivores (intermediate host). However, sarcocysts have been found in muscles of wild and domestic carnivores suggesting they are intermediate host for some Sarcocystis spp. Here, we report mature sarcocysts in the muscles of Pampas fox (Lycalopex gymnocercus). A total of 36 free-living foxes were analyzed. Different skeletal muscles were assessed by microscopic and molecular methods. Cysts and/or DNA of Sarcocystis sp...
February 3, 2017: Parasitology International
https://www.readbyqxmd.com/read/28163249/activation-and-exhaustion-of-antigen-specific-cd8-t-cells-occur-in-different-splenic-compartments-during-infection-with-plasmodium-berghei
#14
Ganchimeg Bayarsaikhan, Mana Miyakoda, Kazuo Yamamoto, Daisuke Kimura, Masoud Akbari, Masao Yuda, Katsuyuki Yui
The spleen is the major organ in which T cells are primed during infection with malaria parasites. However, little is known regarding the dynamics of the immune responses and their localization within the splenic tissue during malaria infection. We examined murine CD8(+) T cell responses during infection with Plasmodium berghei using recombinant parasites expressing a model antigen ovalbumin (OVA) protein and compared the responses with those elicited by Listeria monocytogenes expressing the same antigen. OVA-specific CD8(+) T cells were mainly activated in the white pulp of the spleen during malaria infection, as similarly observed during Listeria infection...
February 3, 2017: Parasitology International
https://www.readbyqxmd.com/read/28137668/praobdellidae-hirudinida-arhynchobdellida-is-not-specific-only-to-the-mucous-membrane-after-all-discovery-of-a-praobdellid-leech-feeding-on-the-japanese-freshwater-crab-geothelphusa-dehaani
#15
Takafumi Nakano, Ko Tomikawa, Takahiro Sakono, Natsuhiko Yoshikawa
Praobdellid leech species have been known to infest vertebrate mucous-membrane; some of them have been assumed to be invertebrate bloodsuckers. Praobdellid individuals were found feeding on the Japanese freshwater crab, Geothelphusa dehaani, at Mt. Tsukuba, Ibaraki Prefecture, Japan. The leech had inserted its head into the intersegmental membrane between the crab's carapace and legs. Our findings represent a first invertebrate host record for praobdellid leeches. Additionally, molecular phylogenetic trees based on nuclear 18S and 28S rRNA, mitochondrial cytochrome c oxidase subunit I, and 12S rRNA sequences as well as the leech morphological characteristics showed that the present leech might belong to an unrecognized praobdellid lineage: a taxonomic revision of the Japanese praobdellid species is needed...
January 28, 2017: Parasitology International
https://www.readbyqxmd.com/read/28137670/effects-of-thai-piperaceae-plant-extracts-on-neospora-caninum-infection
#16
Arpron Leesombun, Sookruetai Boonmasawai, Yoshifumi Nishikawa
Neosporosis has a worldwide distribution and causes economic losses in farming, particularly by increasing the risk of abortion in cattle. This study investigated the effects of Thai piperaceae (Piper betle, P. nigrum, and P. sarmentosum) extracts on Neospora caninum infections in vitro and in vivo. In an in vitro parasite growth assay based on the green fluorescent protein (GFP) signal, P. betle was the most effective extract at inhibiting parasite growth in human foreskin fibroblast cells (IC50 of GFP-expressing N...
January 27, 2017: Parasitology International
https://www.readbyqxmd.com/read/28137669/effective-gene-delivery-to-trypanosoma-cruzi-epimastigotes-through-nucleofection
#17
Lisandro Pacheco-Lugo, Yirys Díaz-Olmos, José Sáenz-García, Christian Macagnan Probst, Wanderson Duarte DaRocha
New opportunities have raised to study the gene function approaches of Trypanosoma cruzi after its genome sequencing in 2005. Functional genomic approaches in Trypanosoma cruzi are challenging due to the reduced tools available for genetic manipulation, as well as to the reduced efficiency of the transient transfection conducted through conventional methods. The Amaxa nucleofector device was systematically tested in the present study in order to improve the electroporation conditions in the epimastigote forms of T...
January 27, 2017: Parasitology International
https://www.readbyqxmd.com/read/28132801/high-pathogenicity-and-strong-immunogenicity-of-a-chinese-isolate-of-eimeria-magna-p%C3%A3-rard-1925
#18
Geru Tao, Yunzhou Wang, Chao Li, Xiaolong Gu, Ping Cui, Sufang Fang, Xun Suo, Xianyong Liu
Coccidia infection of rabbits with one or several species of parasites of the genus Eimeria causes coccidiosis, a disease leading to huge economic losses in the rabbit industry. Eimeria magna, one of the causal agents of rabbit coccidiosis, was characterized as mildly pathogenic and moderately immunogenic in previous studies. In this study, we identified a Chinese isolate of E. magna by testing its biological features (oocyst morphology and size, prepatent time) and sequencing its internal transcribed spacer 1 (ITS-1) DNA fragment...
January 27, 2017: Parasitology International
https://www.readbyqxmd.com/read/28131768/brachylaima-ezohelicis-sp-nov-trematoda-brachylaimidae-found-from-the-land-snail-ezohelix-gainesi-with-a-note-of-an-unidentified-brachylaima-species-in-hokkaido-japan
#19
Minoru Nakao, Tsukasa Waki, Mizuki Sasaki, Jason L Anders, Daisuke Koga, Mitsuhiko Asakawa
In the Japanese Archipelago, Ezohelix gainesi, a member of bradybaenid land snails, is endemic mainly to the island of Hokkaido. During July to August of 2016, a survey to detect trematode infections from E. gainesi was carried out at a forest city park in Asahikawa, Hokkaido. Systemic infections of the snails with sporocysts containing short-tailed cercariae were found in 5.3% of 94 individuals examined. Furthermore, most of them (90.4%) harbored non-encysted metacercariae within their kidneys. A DNA sequence identification revealed that both of the sporocyst and the metacercaria belong to an unknown species of the family Brachylaimidae...
January 25, 2017: Parasitology International
https://www.readbyqxmd.com/read/28110081/isolation-and-partial-characterization-of-an-immunogenic-antigen-of-giardia-lamblia
#20
Jael Quintero, Alejandra Valdez, Brenda Samaniego, Gloria Lopez-Romero, Humberto Astiazaran-Garcia, Lucila Rascon, Linda Breci, Adriana Garibay-Escobar, Ramón Robles-Zepeda, Carlos Velazquez
Humoral and cellular immune responses play an important role during Giardia lamblia infection. Several Giardia proteins have been identified as immunogenic antigens based on their elicited humoral immune response. Poorly is known about Giardia antigens that stimulate a cellular immune response. The main purpose of this study was to isolate and partial characterize an immunogenic antigen (5G8) of G. lamblia. The 5G8 protein was isolated from G. lamblia trophozoite lysates by affinity chromatography using moAb 5G8-coupled CNBr-Sepharose...
January 19, 2017: Parasitology International
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