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Clinical Oral Investigations

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https://www.readbyqxmd.com/read/28726036/proteolytic-effects-of-gingipains-on-trefoil-factor-family-peptides
#1
Ponlatham Chaiyarit, Janthima Jaresitthikunchai, Narumon Phaonakrop, Sittiruk Roytrakul, Barbara Potempa, Jan Potempa
OBJECTIVES: The present study was aimed to determine whether trefoil factor family (TFF) peptides which were generally considered to be resistant to proteolysis could be digested by gingipains, a major proteinases produced by Porphyromonas gingivalis. MATERIALS AND METHODS: Recombinant human TFF1, TFF2, and TFF3 peptides were used as substrates. Gingipains including arginine gingipain (RgpB) and lysine gingipain (Kgp) were used as enzymes. Trypsin was used as a control protease...
July 19, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28721528/soft-tissue-profile-changes-after-functional-mandibular-advancer-or-herbst-appliance-treatment-in-class-ii-patients
#2
Jan Hourfar, Jörg Alexander Lisson, Ulrich Gross, Linda Frye, Gero Stefan Michael Kinzinger
OBJECTIVE: The objective of the present study is to compare the effects on soft tissue profile in class II patients after treatment with either "Functional Mandibular Advancer" (FMA) or Herbst appliance. MATERIALS AND METHODS: The study included n = 42 patients treated with either FMA (n = 21) or Herbst appliance (n = 21) by the same experienced orthodontist. The treatment followed a single-step advancement protocol. Lateral cephalograms were analyzed through a set of customized measurements...
July 18, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28721527/fesem-evaluation-of-smear-layer-removal-using-different-irrigant-activation-methods-endoactivator-endovac-pui-and-lai-an-in-vitro-study
#3
Manuele Mancini, Loredana Cerroni, Lorenzo Iorio, Lorenzo Dall'Asta, Luigi Cianconi
OBJECTIVES: This study was to compare the efficacy of EndoActivator, EndoVac, PUI, and LAI methods in removing the smear layer from root canals. MATERIALS AND METHODS: Eighty single-rooted mandibular premolars were decoronated to a standardized length of 15 mm. Specimens were shaped to ProTaper F4 (Dentsply Maillefer) and irrigated with 5.25% NaOCl at 37 °C. Teeth were divided into six groups (two control groups [n = 10] and four test groups [n = 15]) according to the final irrigation activation/delivering technique (sonic irrigation [EndoActivator], passive ultrasonic irrigation [PUI], negative apical pressure [EndoVac], and laser activated irrigation [LAI])...
July 18, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28714041/was-molar-incisor-hypomineralisation-mih-present-in-archeological-case-series
#4
LETTER
Jan Kühnisch
No abstract text is available yet for this article.
July 16, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28710653/mechanical-performance-of-cement-and-screw-retained-all-ceramic-single-crowns-on-dental-implants
#5
Matthias Obermeier, Oliver Ristow, Kurt Erdelt, Florian Beuer
OBJECTIVES: This in-vitro study was performed to compare the contact wear, fracture strength and failure mode of implant-supported all-ceramic single crowns manufactured with various fabrication and fixation concepts. MATERIALS AND METHODS: Fifty dental implants (Conelog Ø 4,3mm/L11mm, Camlog Biotechnologies AG) were embedded and treated with all-ceramic molar single-crowns. Three groups received hand-layered zirconia crowns (IPS e.max Ceram/ IPS e.max ZirCAD, Ivoclar Vivadent AG): CZL (cement-retained zirconia-based layered) group crowns were cemented conventionally, SZL (screw-retained zirconia-based layered) group crowns were screw-retained, MZL (modified zirconia-based layered) group crowns showed a different coping design with screw retention...
July 15, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28710652/comparison-between-inflammation-related-markers-in-peri-implant-crevicular-fluid-and-clinical-parameters-during-osseointegration-in-edentulous-jaws
#6
Amália M Bielemann, Raissa M Marcello-Machado, Fábio Renato Manzolli Leite, Frederico Canato Martinho, Otacílio Luiz Chagas-Júnior, Altair Antoninha Del Bel Cury, Fernanda Faot
OBJECTIVES: The aim of this study is to improve the understanding of interleukin mechanisms during osseointegration to enhance the monitoring of implant failure and success. Clinical parameters, implant stability, and cytokine levels in peri-implant crevicular fluid (PICF) during early bone healing after implant placement were investigated. MATERIAL AND METHODS: Sixty narrow implants were placed in mandible anterior region of 30 edentulous patients (67.23 ± 7...
July 14, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28707226/the-role-of-macrophage-polarization-on-fibroblast-behavior-an-in-vitro-investigation-on-titanium-surfaces
#7
Xuzhu Wang, Yulan Wang, Dieter D Bosshardt, Richard J Miron, Yufeng Zhang
OBJECTIVES: This study investigated the effect of smooth and rough titanium surface topographies on macrophage polarization and their influence on gingival fibroblast behavior cultured on titanium surfaces. MATERIALS AND METHODS: RAW 264.7 macrophages were seeded on smooth (pickled titanium (PT)) and rough Sand-blasted with Large grit particles followed by Acid-etching (SLA) titanium surfaces and first investigated for macrophage polarization towards tissue-inflammatory M1 macrophages or wound-healing M2 macrophages...
July 14, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28699091/the-effects-of-ozone-application-on-genotoxic-damage-and-wound-healing-in-bisphosphonate-applied-human-gingival-fibroblast-cells
#8
Sıdıka Sinem Akdeniz, E Beyler, Y Korkmaz, E Yurtcu, U Ates, K Araz, F I Sahin, O Y Torun
OBJECTIVES: Medication-related osteonecrosis of the jaws (MRONJ) is an extremely therapy-resistant disease involving the jaws especially following bisphosphonate treatment. Bisphosphonates accumulate in bone in concentrations sufficient to be directly toxic to the oral epithelium. Current therapeutic options are inadequate for the prevention and treatment of MRONJ. The aim of this study was to investigate effects of ozone gas plasma therapy on wound healing in bisphosphonate-applied human fibroblasts...
July 11, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28695450/biofunctionalization-of-porcine-derived-collagen-matrix-using-enamel-matrix-derivative-and-platelet-rich-fibrin-influence-on-mature-endothelial-cell-characteristics-in-vitro
#9
Jung Soo Park, Andreas Max Pabst, Maximilian Ackermann, Maximilian Moergel, Junho Jung, Adrian Kasaj
OBJECTIVES: The present study evaluated the effect of an enamel matrix derivative (EMD) and platelet-rich fibrin (PRF)-modified porcine-derived collagen matrix (PDCM) on human umbilical vein endothelial cells (HUVEC) in vitro. MATERIALS AND METHODS: PDCM (mucoderm®) was prepared to 6 mm (±0.1 mm) diameter discs. PDCM samples were incubated with either EMD, PRF, or control solutions for 100 min at 4 °C before the experiments. Cell-inducing properties of test materials on HUVEC cells were tested with cell proliferation assays (MTT, PrestoBlue®), a cytotoxicity assay (ToxiLight®), a Boyden chamber migration assay, and a cell attachment assay...
July 11, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28691145/the-effect-of-saliva-on-the-fate-of-nanoparticles
#10
Birgit J Teubl, Biljana Stojkovic, Dominic Docter, Elisabeth Pritz, Gerd Leitinger, Igor Poberaj, Ruth Prassl, Roland H Stauber, Eleonore Fröhlich, Johannes G Khinast, Eva Roblegg
OBJECTIVES: The design of nanocarriers for local drug administration to the lining mucosa requires a sound knowledge of how nanoparticles (NPs) interact with saliva. This contact determines whether NPs agglomerate and become immobile due to size- and interaction-filtering effects or adsorb on the cell surface and are internalized by epithelial cells. The aim of this study was to examine the behavior of NPs in saliva considering physicochemical NP properties. MATERIALS AND METHODS: The salivary pore-size distribution was determined, and the viscosity of the fluid inside of the pores was studied with optical tweezers...
July 9, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28689366/micro-ct-and-fe-sem-enamel-analyses-of-calcium-based-agent-application-after-bleaching
#11
Mauricio Neves Gomes, Flávia Pires Rodrigues, Nick Silikas, Carlos Eduardo Francci
OBJECTIVES: The objective of the present study is to evaluate the effects of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) on bleached enamel. MATERIALS AND METHODS: A bleaching agent (35% hydrogen peroxide) was applied, 4 × 8 min on premolar teeth (n = 8). A CPP-ACP paste was applied for 7 days. Prior and post-treatment, microtomography images were obtained and 3D regions of interest (ROIs) were selected, from outer enamel, extending to 110...
July 8, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28689365/compromised-inflammatory-cytokine-response-to-p-gingivalis-lps-by-fibroblasts-from-inflamed-human-gingiva
#12
Tracy R Fitzsimmons, Shaohua Ge, P Mark Bartold
OBJECTIVES: The aims of this study were to compare the in vitro cytokine response of gingival fibroblasts (GF's) from healthy and inflamed human gingival tissues and to assess whether GF's from inflamed gingivae are capable of mounting a secondary inflammatory response after exposure to P. gingivalis LPS. MATERIALS AND METHODS: GF's were obtained from healthy donors and periodontitis patients and cultured in vitro. Cells were exposed to P. gingivalis LPS for 24h before measurement of MCP-1, GRO, IL-6, IL-8 and VEGF using a bead-based multiplex assay...
July 8, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28688093/influence-of-bisphosphonates-on-the-adherence-and-metabolism-of-epithelial-cells-and-gingival-fibroblasts-to-titanium-surfaces
#13
Fernanda Gonçalves Basso, Taisa N Pansani, Diana G Soares, Lais M Cardoso, Josimeri Hebling, Carlos Alberto de Souza Costa
OBJECTIVES: To evaluate the effects of sodium alendronate (SA) and zoledronic acid (ZA), on the adhesion and metabolism of epithelial cells and gingival fibroblasts to titanium surfaces considering cell functions related to an effective mucosal barrier around the implant. MATERIALS AND METHODS: Cells were seeded onto titanium discs and incubated for 24 h. Then, serum-free DMEM containing selected bisphosphonates (0, 0.5, 1, or 5 μM) was added for 24 and 48 h...
July 8, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28688092/mechanical-loading-increases-pro-inflammatory-effects-of-nitrogen-containing-bisphosphonate-in-human-periodontal-fibroblasts
#14
Collin Jacobs, Sabrina Schramm, Isabelle Dirks, Christian Walter, Andreas Pabst, Dan Meila, Cornelius Jacobs, Heinrich Wehrbein
OBJECTIVES: There is increasing evidence that inflammation and biomechanical loading can influence the effects of bisphosphonates (BP). The aim of this study was to investigate the influence of tensile strain application combined with IL-1ß and clodronate or zoledronate on human periodontal ligament fibroblasts (HPdLF) in vitro. MATERIALS AND METHODS: HPdLF were cultured with 10 nM IL-1ß and 5 μM clodronate or zoledronate for 48 h. Cells were applied to cyclic tensile strain (CTS; 3% elongation) for 12 h in vitro...
July 7, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28688091/erratum-to-clinical-comparison-of-the-effectiveness-of-7-and-14-day-intracanal-medications-in-root-canal-disinfection-and-inflammatory-cytokines
#15
Frederico C Martinho, Cinthya C Gomes, Gustavo G Nascimento, Ana P M Gomes, Fábio R M Leite
No abstract text is available yet for this article.
July 7, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28681248/sinus-floor-elevation-using-implants-coated-with-recombinant-human-bone-morphogenetic-protein-2-micro-computed-tomographic-and-histomorphometric-analyses
#16
Daniel S Thoma, So-Ra Yoon, Jae-Kook Cha, Hyun-Chang Lim, Jung-Seok Lee, Seong-Ho Choi, Ui-Won Jung
OBJECTIVES: The objective of this study was to determine the validity of a graft-free sinus floor elevation (SFE) procedure with simultaneous placement of recombinant morphogenetic protein-2 (rhBMP-2)-coated implants compared to uncoated control implants. METHODS: In 10 rabbits, SFE was performed on both sides. Dental implants were randomly placed in the sinus filled with a blood clot. Test implants were coated with rhBMP-2, whereas in the control group, implants were uncoated...
July 6, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28681247/impact-of-manual-control-point-selection-accuracy-on-automated-surface-matching-of-digital-dental-models
#17
Kathrin Becker, Benedict Wilmes, Chantal Grandjean, Dieter Drescher
OBJECTIVES: Treatment outcomes are frequently evaluated based on the superimposition of digital dental models. However, errors from surface matching may distort these findings. The aims of this study were (i) to develop a simulation unit to mimic point set registrations and (ii) to evaluate the impact and clinical relevance of manual landmark selection errors on registration accuracy. MATERIAL AND METHODS: Ten randomly selected dental casts were digitized using a 3D laser scanner, and were loaded by an in-house developed simulation unit (MATLAB R2014a)...
July 5, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28676903/estimated-prevalence-of-halitosis-a-systematic-review-and-meta-regression-analysis
#18
REVIEW
Manuela F Silva, Fábio R M Leite, Larissa B Ferreira, Natália M Pola, Frank A Scannapieco, Flávio F Demarco, Gustavo G Nascimento
OBJECTIVE: This study aims to conduct a systematic review to determine the prevalence of halitosis in adolescents and adults. METHODS: Electronic searches were performed using four different databases without restrictions: PubMed, Scopus, Web of Science, and SciELO. Population-based observational studies that provided data about the prevalence of halitosis in adolescents and adults were included. Additionally, meta-analyses, meta-regression, and sensitivity analyses were conducted to synthesize the evidence...
July 4, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28676902/finding-an-upper-limit-of-what-might-be-achievable-by-patients-oral-cleanliness-in-dental-professionals-after-self-performed-manual-oral-hygiene
#19
Renate Deinzer, René Schmidt, Daniela Harnacke, Jörg Meyle, Dirk Ziebolz, Thomas Hoffmann, Bernd Wöstmann
AIMS: Though patients have been shown to have difficulties in achieving oral cleanliness after self-performed oral hygiene, scientifically and empirically justified standards for the degree of oral cleanliness they should achieve are lacking. Oral cleanliness of dental staff was therefore assessed as an indicator of what might be an upper limit of what can be expected by patients. MATERIALS AND METHODS: In a multicentre study, N = 64 university dentists, N = 33 dental students and N = 30 dental assistants were asked to perform manual oral hygiene to the best of their abilities...
July 4, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28674819/does-a-face-bow-lead-to-better-occlusion-in-complete-dentures-a-randomized-controlled-trial-part-i
#20
Manja von Stein-Lausnitz, Guido Sterzenbach, Iven Helm, Antje Zorn, Felix H Blankenstein, Sebastian Ruge, Bernd Kordaß, Florian Beuer, Ingrid Peroz
OBJECTIVES: In a double-blinded randomized controlled clinical trial, the impact of face-bow registration for remounting complete dentures (CDs) on the occlusal parameters (part I) was evaluated. MATERIALS AND METHODS: New CDs of 32 patients were duplicated and mounted after intraoral pin registration according to mean settings (group 1) and (group 2) using a face-bow (arbitrary hinge axis). The vertical dimension was reduced to the first occlusal contact point, and a bite record was fabricated in the articulator...
July 4, 2017: Clinical Oral Investigations
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