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Clinical Laboratory

Mesude Falay, Gul E Ulusan, Mehmet Şenes, Ismail O Acar
No abstract text is available yet for this article.
June 1, 2018: Clinical Laboratory
Maria Salinas, Maite Lopez-Garrigos, Emilio Flores, Carlos Leiva-Salinas
BACKGROUND: To study the urinalysis request, pre-analytical sample conditions, and analytical procedures. METHODS: Laboratories were asked to provide the number of primary care urinalyses requested, and to fill out a questionnaire regarding pre-analytical conditions and analytical procedures. RESULTS: 110 laboratories participated in the study. 232.5 urinalyses/1,000 inhabitants were reported. 75.4% used the first morning urine. The sample reached the laboratory in less than 2 hours in 18...
June 1, 2018: Clinical Laboratory
Elisabeth H Adam, Kai Zacharowski, Gudrun Hintereder, Florian Raimann, Patrick Meybohm
BACKGROUND: Blood loss due to phlebotomy leads to hospital-acquired anemia and more frequent blood transfusions that may be associated with increased risk of morbidity and mortality in critically ill patients. Multiple blood conservation strategies have been proposed in the context of patient blood management to minimize blood loss. Here, we evaluated a new small-volume sodium citrate collection tube for coagulation testing in critically ill patients. METHODS: In 46 critically adult ill patients admitted to an interdisciplinary intensive care unit, we prospectively compared small-volume (1...
June 1, 2018: Clinical Laboratory
Mai Kumeta, Kazuto Tanaka, Natsuki Kaneko, Takayuki Osanai, Hikaru Ajima, Yoshiko Tamai, Hiroyuki Kayaba, Etsuro Ito, Norihiro Saito
BACKGROUND: Only a few cases of infantile anti-red blood cell alloantibody production have been reported. METHODS: A 7-month-old girl with acute lymphoid leukemia developed anti-E alloantibody 13 days after transfusion of E-positive red blood cells. Antibody screening was performed before and at 2, 6, 13, 18, 27, 34, and 49 days after red blood cell transfusion. Identification test, direct immunoglobulin test, acid elution, and dithiothreitol test were also performed...
June 1, 2018: Clinical Laboratory
Tingting Liang, Zhifeng Han, Huan Zhao, Xuan Zhang, Yao Wang
BACKGROUND: Our previous studies revealed that concentrations of circulating antibodies to annexin A1 (ANXA1) were increased in non-small lung cancer (NSCLC). This study was thus designed to replicate this initial finding with an independent sample set. METHODS: An enzyme-linked immunosorbent assay (ELISA) was developed in-house to examine plasma antiANXA1 IgG levels in 220 patients with NSCLC and 200 control subjects. RESULTS: Mann-Whitney U test showed that patients with NSCLC had significantly higher anti-ANXA1 IgG levels than control subjects (Z = -4...
June 1, 2018: Clinical Laboratory
Lili Zhang, Bo Pang, Wenbin Zhang, Wei Bai, Weiying Yu, Yuanyuan Li, Wanqing Hua, Wenjun Li, Changgui Kou
BACKGROUND: DNA demethylase is a crucial enzyme in the epigenetic modification and regulation mechanisms of gene transcription. Based on previous assertions that the pathophysiology of schizophrenia is associated with epigenetics, we aimed to explore whether DNA demethylase activity might be related to schizophrenia in northeast China. METHODS: We recruited 25 patients with first-episode schizophrenia and 29 normal controls from a northeast Chinese Han population...
June 1, 2018: Clinical Laboratory
Sonja Hamed, Michael Behnes, Dominic Pauly, Dominic Lepiorz, Max Barre, Tobias Becher, Siegfried Lang, Ibrahim Akin, Martin Borggrefe, Thomas Bertsch, Ursula Hoffmann
BACKGROUND: The prognostic value of the acute phase protein Pentraxin 3 (PTX-3) is not well evaluated in patients with septic shock, which reveal an unacceptably high short- and long-term mortality. New Sepsis-3 definitions are not yet implemented in most biomarker studies. Therefore, this study assesses the prognostic value of PTX-3 for short- and mid-term mortality in patients with sepsis or septic shock, as defined by the latest definitions, treated at a medical intensive care unit (ICU)...
June 1, 2018: Clinical Laboratory
Yunxin Zeng, Jingwen Zhang, Xiaoqing Li, Ling Zhang, Jiajun Liu
BACKGROUND: T315I mutation is the most common BCR-ABL mutation and confers resistance to all the first and second generation BCR-ABL tyrosine kinases, including nilotinib and dasatinib. We report a high risk chronic myelogenous leukemia (CML) patient harboring the T315I mutation treated by Interferon-α (INF-α) solo and subsequently combined with dasatinib. METHODS: Hematological investigation, bone marrow cytology inspection, chromosomal analysis (G-banding), and real-time quantitative polymerase chain reaction (RQ-PCR) were performed on a 47-year-old male patient...
June 1, 2018: Clinical Laboratory
Marta E Martinuzzo, Cristina Duboscq, Marina S Lopez, Luis H Barrera, Estela S Vinuales, Jose Ceresetto, Ricardo R Forastiero, Jose Oyhamburu
BACKGROUND: Rivaroxaban oral anticoagulant does not need laboratory monitoring, but in some situations plasma level measurement is useful. The objective of this paper was to verify analytical performance and compare two rivaroxaban calibrated anti Xa assays/coagulometer systems with specific or other branch calibrators. METHODS: In 59 samples drawn at trough or peak from patients taking rivaroxaban, plasma levels were measured by HemosIL Liquid anti Xa in ACLTOP 300/500, and STA liquid Anti Xa in TCoag Destiny Plus...
June 1, 2018: Clinical Laboratory
Panpan Zhao, Hongliang Zhang, Ying Zhang, Li Sun
BACKGROUND: Marchiafava-Bignami disease (MBD) is a neurological degenerative disorder with a pathognomonic hallmark of symmetric demyelination in the corpus callosum (CC). Most reported cases were chronic alcoholics and some showed cortical lesions related to poor clinical prognosis. Herein we report a case of a chronic alcoholic who presented with confusion and generalized weakness. METHODS: Nerve fiber integrity and metabolic changes were evaluated with Magnetic resonance imaging (MRI) sequences including diffusion tensor imaging (DTI) and MRS...
June 1, 2018: Clinical Laboratory
Xuqin Wang, Ting Yang, Jingkun Miao, Huan Liu, Kaifeng Wu, Jing Guo, Jie Chen, Tingyu Li
BACKGROUND: Offspring of mothers with gestational diabetes mellitus (GDM) are far more likely to develop type 2 diabetes. The aim of this study was to investigate the effect of the insulin metabolism of pregnant women with GDM in late pregnancy on the insulin metabolism of the fetuses and their correlation. METHODS: This study enrolled 55 pregnant women with GDM and 87 control subjects. Fasting venous blood samples and umbilical venous blood samples (reflecting fetal metabolism) were collected from the study subjects...
June 1, 2018: Clinical Laboratory
Yu Du, Yong-Sheng Tu, Yong-Bo Tang, Yun-Ying Huang, Fang-Min Zhou, Tian Tian, Xiao-Yan Li
BACKGROUND: ClC-3 is involved in the proliferation and migration of several cancer cells. However, ClC-3 expression and its role of cell-cycle control in multiple myeloma (MM) has not yet been investigated. METHODS: MM cells were treated with different concentrations of IGF (30, 100, 300 ng/mL), and their proliferation was examined by CCK-8. The effects of ClC-3 on cell cycle progression was detected by flow cytometry. Western blot was used to analyze the relative levels of ClC3, CD138, P21, P27, CDK, p-Erk1/2, and t-Erk1/2 protein expression...
June 1, 2018: Clinical Laboratory
Farideh Noori, Sirous Naeimi, Mohammad J Zibaeenezhad, Fatemeh R Gharemirshamlu
BACKGROUND: Myocardial infarction (MI) is an irreversible damage of myocardial tissue caused by prolonged ischemia and hypoxia. A local hypoxia-induced inflammation causes recruitment of leukocytes to the inflammatory site to aid cardiac remodeling and tissue healing. Among various chemokines involved in the process, CCL22 plays an essential role in cardiac cell migrations. In this study, we evaluated the incidence of rs4359426 and rs2228428 SNPs in CCL22/CCR4 genes of MI patients and studied their association with the physiology of the disease...
June 1, 2018: Clinical Laboratory
Hui Zhu, Teng Zhao, Jingyao Liu
BACKGROUND: Paraoxonase1 (PON1) is an antioxidant which confers antioxidant properties to high-density lipoprotein (HDL) and prevents low-density lipoprotein (LDL) oxidation. The purpose of this study was to evaluate the activities of PON1 and oxidative/antioxidative stress markers in acute cerebral infarction. METHODS: In this study, 161 patients diagnosed with acute cerebral infarction and 161 gender- and age-matched healthy controls were recruited. Based on the clinicoradiological profiles, the patients were further classified into two groups: lacunar infarction group and large-artery atherosclerosis group...
June 1, 2018: Clinical Laboratory
Guang-Li Xu, Xiao-Fang Hu, Yong-Mei Han, Ai-Wu Wei
BACKGROUND: To study the clinical effect of low molecular heparin on unexplained recurrent spontaneous abortion (URSA). METHODS: A total of 120 URSA patients were collected in our hospital from October 2015 to September 2017. They were divided into two groups: control group (n = 60) and observation group (n = 60). The patients in the control group were administered with progesterone and human chorionic gonadotropin, and the observation group with low molecular heparin...
June 1, 2018: Clinical Laboratory
Piao Jing-Zi, He Zheng-Xin, Chen Wei-Jun, Jiang Yong-Qiang
BACKGROUND: Acute bacterial meningitis remains a life-threatening infectious disease with considerable morbidity and mortality. DNA-based detection methods are an urgent requisite for meningitis-causing bacterial pathogens for the prevention of outbreaks and control of infections. METHODS: We proposed a novel PCR-mass spectrometry (PCR-Mass) assay for the simultaneous detection of four meningitis-causing agents, Neisseria meningitidis, Streptococcus pneumoniae, Haemophilus influenzae, and Mycobacterium tuberculosis in the present study...
June 1, 2018: Clinical Laboratory
Edip Erkus, Mehmet Z Kocak, Gulali Aktas, Mehmet Ozen, Burcin M Atak, Tuba T Duman, Buket K Tekce, Haluk Savli
BACKGROUND: Cold agglutinin disease is a very rare condition associated with agglutination of erythrocytes in cold environment usually due to IgM type antibodies. Other than hemolytic anemias, it may interfere with routine hemogram tests due to miscalculation of red blood cell count (RBC) and other hemogram parameters calculated with involvement of RBC. Awareness of the condition is important to overcome laboratory errors. METHODS: We studied a peripheral blood smear and repeated the hemogram test at 37°C to establish the diagnosis of cold agglutinin disease...
June 1, 2018: Clinical Laboratory
Wenjun Tang, Chunhua Tang, Jianfeng Zheng, Yongling He, Fangfang Lu
BACKGROUND: Pseudothrombocytopenia, caused by platelet (PLT) clumping, is often found in clinical studies [1]. However, pseudothrombocytosis resulting from the fragmentation of red blood cells (RBCs) is a very rare phenomenon. METHODS: EDTA-K2 anticoagulation was used on a sample of venous blood extracted from the patient. A Symex XN9000 automatic blood analyzer was used to conduct CBC + DIFF mode and CBC + DIFF + RET mode tests, stained smear microscopy. RESULTS: The Symex XN9000 automatic blood analyzer was used to conduct CBC + DIFF mode test; PLTs were measured at 570 x 109/L...
June 1, 2018: Clinical Laboratory
A Biaz, J Uwingabiye, A Rachid, A Dami, S Bouhsain, Z Ouzzif, S El Machtani Idrissi
BACKGROUND: We report a case of immunoglobulin (Ig) D myeloma with hidden lambda light chains in a patient whose immunofixation test was very difficult to interpret: the IgD reacts with the anti-δ heavy chain antiserum but does not react with anti-lambda antiserum. The band in the D heavy chain lane is unmatched in light chain lanes and the band in lambda light chain lane migrates higher. METHODS: To distinguish between heavy chain disease and immunoglobulin with "hidden" light chains, the sample was exposed to a very high concentration of anti-lambda and anti-kappa antisera for 48 hours...
June 1, 2018: Clinical Laboratory
Yuan Dang, Xudong Wei, Laien Xue, Fuli Wen, Jianjun Gu, Heping Zheng
BACKGROUND: Glioma is one of the most common and aggressive malignant tumors of the central nervous system. METHODS: Here, we review and explore the use of long noncoding RNA (lncRNA) as a therapeutic strategy for the targeting of gliomas. RESULTS: LncRNA is a functional RNA molecule with no protein coding function and is involved in the occurrence and progression of glioma. It is reported that the activation of several signaling pathways, including the MAPK, p53, Wnt/β-catenin, PI3K/AKT/mTOR, and epithelial mesenchymal transformation (EMT) pathways, are involved in the regulation of gliomas...
June 1, 2018: Clinical Laboratory
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