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Clinical Laboratory

Teofana O Bizerea, Ramona Stroescu, Alexandru F Rogobete, Otilia Marginean, Constantin Ilie
BACKGROUND: Pregnancy-induced hypertension (PIH) is a multifactorial disorder that increases the risk of morbidity and mortality in both mother and newborn. Although there are many studies that track the effects of PIH on maternal and neonatal outcome, the results are contradictory. This retrospective study focuses on the effect of maternal PIH on neonatal hematological changes (thrombocytopenia and neutropenia). It also tries to determine whether neonatal thrombocytopenia and neutropenia are direct consequences of maternal PIH, rather than of the small for gestational age (SGA) status of the newborn...
July 1, 2018: Clinical Laboratory
Mehmet Demirci, Bilun Gemicioglu, Suat Saribas, Zeynep Taner, Muzeyyen Mamal-Torun, Belma Karatoka, Linda Asik-Cagli, Ozlem Guven, Suleyman Demiryas, Bekir S Kocazeybek, Hrisi Bahar-Tokman
BACKGROUND: Parapneumonic effusions usually occur secondary to an infection and produce pus (empyema) that accumulates in the pleural space. We aimed to evaluate the prevalence of anerobes in patients with empyema and to assess their resistance patterns for seven antimicrobials. METHODS: Pleural fluid specimens from 236 patients were inoculated on Schaedler agar. Anaerobic bacteria were identified via API 20 A. Susceptibility testing for penicillin, ampicillin + sulbactam, amoxicillin + clavulanate, cefoxitin, clindamycin, metronidazole, and imipenem were performed with the E-test...
July 1, 2018: Clinical Laboratory
Linfeng Chen, Yue Wang, Wei Wang, Meixiang Zhang, Hongxing Cai
BACKGROUND: Recently, esophageal cancer has become more common in China. To find a molecular biomarker will provide a handy way to improve precancerous diagnosis and evaluate the state of lymph node metastasis, improving prognosis. The present study aimed to investigate the expression level of hsa-miR-6743-5p in 25 esophageal tissues and to estimate the correlation between clinicopathological features of esophageal squamous cell cancer with miR-6743-5p expression. METHODS: Quantitative reverse transcription polymerase chain reaction was performed to examine the expression level of miR-6743-5p in 25 pairs of esophageal cancer tissues and adjacent non-cancerous tissues...
July 1, 2018: Clinical Laboratory
Meng Jiang, Yutong Yin, Lianna Xie, Huozhen He
BACKGROUND: This study aims to evaluate the expression of plasma miR-18, thereby evaluating its potential use as a biomarker to screen acute myocardial infarction (AMI) patients from healthy controls. METHODS: Real time PCR was carried out to evaluate the level of miR-18 in AMI patients and healthy controls. ROC analysis was performed to evaluate whether miR-18 could be used as a potential biomarker. Dual luciferase assay was used to identify the potential target of miR-18...
July 1, 2018: Clinical Laboratory
Huozhen He, Haichao Wang, Fuyang Pei, Meng Jiang
BACKGROUND: This main aim of the study was to evaluate the expression and specific role of miR-543 in the progression of chronic obstructive pulmonary disease (COPD), thereby evaluating their diagnostic ability and treatment in COPD patients. METHODS: Real time PCR was carried out to explore the level of miR-543 in the plasma and lung tissues of COPD patients and controls. ELISA was performed to analyze the level of interleukin-33 (IL-33). Dual luciferase was used to validate the target gene of miR-543...
July 1, 2018: Clinical Laboratory
Raluca Dumache, Veronica Ciocan, Camelia Muresan, Alexandra Enache
BACKGROUND: The aim of the present study was the identification of an unknown person found in an advanced decomposed state using DNA samples provided by two alleged brothers as reference samples. To obtain an increased reliability of the test, we used autosomal and Y-STR markers. METHODS: Tissue fragments were obtained for the DNA isolation during the autopsy examination from the unidentified person. DNA was isolated from the reference samples obtained from buccal swabs of the two alleged brothers...
July 1, 2018: Clinical Laboratory
Yan-Yan Ma, Yu-Peng Liu, Dongxiao Li, Xi-Yuan Li, Jin-Qing Song, Yan-Ling Yang
BACKGROUND: Cystinuria is a rare inherited renal stone disease caused by mutations in the SLC3A1 and SLC7A9 genes. The Chinese cystinuria phenotype and genotype have rarely been reported in the literature. METHODS: For this research, the clinical features and genetic etiology were analyzed in seven children, and the clinical characteristics were summarized. The blood and urine amino acids and acylcarnitine were analyzed. Additionally, the whole coding sequence and exon-intron junctions of the SLC3A1 and SLC7A9 genes were analyzed...
July 1, 2018: Clinical Laboratory
Shiva Poorang, Shokoufeh Abdollahi, Zahra Anvar, Seyed M B Tabei, Bahia-Namavar Jahromi, Najmeh Moein-Vaziri, Behrouz Gharesi-Fard, Mahnaz Banaei, Seyed A Dastgheib
BACKGROUND: Recurrent pregnancy loss (RPL) defined as three or more consecutive spontaneous miscarriages before the 20th week of gestation is caused by different factors including genetic and epigenetic background. However the involvement of paternal background on RPL is an interesting novel argument, which is not well studied. The main focus of the present study was to investigate for the association of paternal methylenetetrahydrofolate reductase (MTHFR) epigenotypes with sperm parameters and RPL...
July 1, 2018: Clinical Laboratory
Laura Heireman, Jan Stroobants, Wim Uyttenbroeck, Yvonne Goossens, Christa Dreezen, Dave Luyts, Lutgarde Van Den Broeck, Joris Delanghe, Evelien Heylen, Boris Mahieu
BACKGROUND: The use of pneumatic tube system (PTS) transport has gained considerable popularity in modern hospitals but is also associated with sample hemolysis. The potential contribution of PTS-associated acceleration forces to high hemolysis rates observed in the emergency department (ED) has not been investigated before and can be easily examined nowadays using smartphone applications. The first aim of our study was to investigate whether our PTS induces hemolysis of patient samples obtained from our ED...
July 1, 2018: Clinical Laboratory
Aidan Huang, Chunni Huang, Meiyu Zhang, Xuan Zhu, Xue Qin
BACKGROUND: The aim of this study was to use a lectin microarray to detect differential glycan profiling of serous glycoprotein in iron overload thalassemia patients. METHODS: This study enrolled iron overload α/β-thalassemia patients and no iron overload α/β-thalassemia patients. Lectin microarray was used to detect the alteration of protein glycosylation. The reliability of the lectin microarray results was verified by the lectin blotting technique. Expression level of hepcidin, erythropoietin, ferritin, and transferrin were measured by western blotting...
July 1, 2018: Clinical Laboratory
Yan-Long Hou, Chong-Ge You
BACKGROUND: High-resolution melting curve analysis has been widely applied as one of the mainstream technologies used for scanning and detecting genetic mutations. After having realized the integration of PCR amplification and HRM detection on the same instrument, researchers have begun to focus on the throughput and speed of PCR-HRM detection. METHODS: A systematic literature search was conducted with the use of PubMed and ScienceDirect for articles on high resolution melting curve and microfluidic technologies...
July 1, 2018: Clinical Laboratory
Fujun Wang, Zongjun Wang, Zengnan Mo
BACKGROUND: This study explores the role of prostaglandin E2 (PGE2) in the etiology of urolithiasis in acute renal colic by analyzing the PGE2 levels in blood and urine throughout the clinical course of renal colic. METHODS: From June 2015 to November 2017, a total of 60 patients with acute renal colic were enrolled in the study. Blood and urine samples were taken before and after administration of drug to inhibit prostaglandin synthesis; further blood and urine samples were obtained from the patients 2 weeks after their recovery...
July 1, 2018: Clinical Laboratory
Jong Ho Lee, Chae Hoon Lee, Il Kwon Bae
BACKGROUND: IMP-4 class B metallo-β-lactamase-producing Enterobacteriaceae are resistant to carbapenems. The aim of this study was to characterize of IMP-4 metallo-β-lactamase (MBL)-producing Enterobacter aerogenes clinical isolate. METHODS: IMP-4 MBL-producing E. aerogenes clinical isolate was collected from a Korean Hospital in 2017. Antimicrobial susceptibility was determined by disk diffusion methods. Further, minimum inhibitory concentrations of β-lactams were determined by Etest...
July 1, 2018: Clinical Laboratory
Kengo Moriyama
BACKGROUND: Levels of uric acid (UA) and low-density lipoprotein (LDL) subclasses are both associated with coronary heart disease and metabolic syndrome (MetS). However, the relationship between UA and LDL subclasses is not well understood. METHODS: Subjects included 633 Japanese subjects not receiving medication for hyperuricemia, diabetes mellitus, dyslipidemia, or chronic renal disease who underwent an annual health examination that included determination of small, dense low-density lipoprotein cholesterol (sdLDL-C) and malondialdehyde-modified low-density lipoprotein (MDA-LDL) levels...
July 1, 2018: Clinical Laboratory
Ling Zhang, Xiaozhen Wang, Na Cheng, Jingwen Zhang, Zhigang Fang, Bing Long, Dongjun Lin
BACKGROUND: Diffuse large B cell lymphoma (DLBCL) and follicular cell lymphoma (FL) synchronously occurring in the same individual is unusual. The authors describe a rare case of DLBCL coexisting with FL at diagnosis in a male patient, with intestinal and lymph node involvement. METHODS: Hematologic investigation, intestinal tumor biopsy, bone marrow examination, cytogenetic analysis, and PCR were performed. RESULTS: The patient received three courses of R-CHOP regimen chemotherapy, the mesentery lymph nodes were reduced to 2...
July 1, 2018: Clinical Laboratory
Youqiong Li, Mao Tian, Ting Qin, Likai Wan
BACKGROUND: Hb I and Hb Shaare Zedek are rare hemoglobin variants in the Chinese population. High-performance liquid chromatography (HPLC) is a widely used technique for screening of thalassemias and hemoglobin variants in Chinese primary hospitals. However, some rare hemoglobin variants cannot be effectively separated by HPLC. Here, we report one case of Hb I and one case of Hb Shaare Zedek which could not be detected by HPLC but required capillary electrophoresis (CE) in our hospital...
July 1, 2018: Clinical Laboratory
Lei Huang, Binghuai Lu, Ranran Qin, Liying Sun
BACKGROUND: PCR-ESI/MS is a commercial molecular method that can identify bloodstream infection (BSI) pathogens directly from blood. Previous studies showed its sensitivity varied greatly. Its diagnostic accuracy has not been systematically evaluated yet, thus we aimed to assess its accuracy by systematic review and meta-analysis. METHODS: Studies were searched on PubMed and Embase up to November 2017, for studies using PCR/ESI-MS to diagnose BSI directly from blood and providing sufficient data to construct two-by-two tables...
July 1, 2018: Clinical Laboratory
Nairo M Sumita, Carlos E S Ferreira, Marines D V Martino, Carolina N Franca, Adriana C L Faulhaber, Marileia Scartezini, Joao R R Pinho, Claudia M Dias, Katia R Cesar, Vitor M Pariz, Joao C C Guerra, Ismar V Barbosa, Marcelo H W Faulhaber, Marcelo C Batista, Adagmar Andriolo, Maria E Mendes, Antonia M O Machado, Marjorie P Colombini, Natasha Slhessarenko, Wilson Shcolnik, Cristina Khawali, Gustavo A Campana, Fernando Berlitz, Cesar A Galoro
BACKGROUND: The use of point-of-care testing (POCT) in different clinical applications is justified by the fact that the time to release the result is shortened, allowing the physician to define the diagnosis and most appropriate therapy in a shorter time. However, the negative aspects must also be highlighted and studied so that we can move forward with the use of these devices. These negative aspects include greater analytical imprecision compared to laboratory automation, the variability between different equipment from different manufacturers, the risk of inappropriate use, a low level of global regulation, higher costs compared with laboratory testing and cost ineffectiveness in terms of health care...
July 1, 2018: Clinical Laboratory
Susan J Louw, Yuen O Wan, Elizabeth S Mayne
BACKGROUND: The validity of laboratory results depends on pre-analytical variables not detected by conventional quality control. Recommendations are for post-centrifugation coagulation samples to remain capped with cappiercing primary tube analysis. Total laboratory automation integrates analytical platforms with potential incompatibilities necessitating changes including pre-analytical uncapping of samples. METHODS: Samples analyzed for Prothrombin Time (PT), activated Partial Thromboplastin Time (aPTT), D-dimers, Antithrombin and Fibrinogen at baseline, and after 60 and 120 minutes were left at ambient temperature, either re-capped or uncapped, in order to simulate changes from baseline that could occur in uncapped samples on an automation track prior to analysis...
July 1, 2018: Clinical Laboratory
Lili Meng, Guiqin Li, Xiaoqin Liu, Juan Jiang, Min Zhu, Yuanbo Sun
BACKGROUND: The current study aims to evaluate the expression pattern changes of miR-199-3p in diabetic nephropathy (DN) patients and explore whether it could be used as a potential biomarker. METHODS: Real time PCR analysis was performed to examine the level of miR-199-3p in normoalbuminuria group (diabetes mellitus [DM]), microalbuminuria group (DNE), macroalbuminuria group (DNC), and healthy controls. ROC analysis was carried out to determine whether urine miR-199-3p could be used as a potential biomarker...
July 1, 2018: Clinical Laboratory
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