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Clinical Laboratory

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https://www.readbyqxmd.com/read/30336540/early-detection-of-colorectal-cancer-a-multi-center-pre-clinical-case-cohort-study-for-validation-of-a-combined-dna-stool-test
#1
Matthias M Dollinger, Susanna Behl, Wolfgang E Fleig
BACKGROUND: Although colonoscopy-based screening has proven to be highly effective in detecting colorectal cancer (CRC), participation rates remain disappointing. Development of CRC is associated with a number of genetic or somatic mutations. New, non-invasive stool tests are currently being developed based on the detection of these alterations. We investigated if a non-invasive stool assay can offer sufficient sensitivity and specificity to supplement colonoscopy-based screening. METHODS: We compared a combined stool assay, which incorporates fecal occult blood testing (FOBT), quantification of human DNA (hDNA) as well as detection of genetic mutations of KRAS and BRAF (Combined DNA stool assay), with commercially available FOBT and M2-PK tests in a multi-centric six-armed pre-clinical case cohort study...
October 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/30336539/the-dynamic-trends-of-urinary-lh-and-fsh-assayed-by-icma-during-triptorelin-stimulation-tests-in-girls-a-pilot-study
#2
Yaping Ma, Shuang Nie, Ze Ben, Zhuangjian Xu, Huan Zhou, Jinling Zhao, Feng Ren, Qing Wang, Shuyan Xie, Tingting Zhang
BACKGROUND: Gonadotropin-releasing hormone stimulation test is a gold standard for evaluating the function of the hypothalamic-pituitary-gonadal axis (HPGA) in children. These tests are usually uncomfortable because of multi-venipunctures. A urine specimen is a good alternative because it is noninvasive and convenient. More studies have shown the correlation between sera and urine LH and FSH levels under different physiological and pathological conditions. METHODS: The study investigated the dynamic trends of urine LH (uLH) and FSH (uFSH) assayed by immunochemiluminometric assays (ICMA) during triptorelin stimulation tests in girls...
October 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/30336538/identification-of-potential-biomarkers-involved-in-gastric-cancer-through-integrated-analysis-of-non-coding-rna-associated-competing-endogenous-rnas-network
#3
Chao Xing, Zhengzhai Cai, Jian Gong, Jun Zhou, Jingjing Xu, Feng Guo
BACKGROUND: Gastric cancer is one of the most common malignant tumors worldwide. Increasing studies have indicated that non-coding RNAs (ncRNAs) play critical roles in cancer progression. They have shown great potential to be useful markers and therapeutic targets. METHODS: Bioinformatics analysis was conducted to detect mRNA and ncRNAs' expression changes between tumor samples and adjacent non-tumor samples. The gene expression profiles were obtained from the national center of biotechnology information gene expression omnibus (GEO)...
October 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/30336537/the-effect-of-over-expression-of-mir-20a-on-cell-proliferation-of-human-t-cell-leukemia-cell-line
#4
Razavi Hashemi M, Vahabpour R, Ranji N, Sanati Mh, Mapar M, Sadat Sm
BACKGROUND: Acute T Cell Leukemia (ALL) is an aggressive and prevalent human malignancy. Chemotherapy is the most frequent therapeutic strategy; however, cytotoxicity and recurrence of cancer are the main concerns. The discovery of microRNA (miRNA) drew the attention of scientists who were working on targeted cancer therapy to use the potential of gene regulation by using this small RNAs. METHODS: miRanda, TargetScan, miRDB databases, and miRWalk software were applied to find probable miRNAs targeting 3'UTR of JAK1, STAT3, SOCS6, AKT1, APAF, BID, and Caspase9 mRNA...
October 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/30336536/could-insulin-and-hemoglobin-a1c-levels-be-predictors-of-hunger-related-malnutrition-undernutrition-without-disease
#5
Seyit Uyar, Suheyla Gorar, Mehmet Kok, Hakan Ozer, Gokhan Koker, Feyzi Bostan, Ayhan H Cekin
BACKGROUND: Malnutrition results in functional changes in the liver and pancreas that negatively affect carbohydrate metabolism. The aim of this study was to evaluate whether insulin hormone and glycated hemoglobin A1c (HbA1c) could serve as predictors of hunger-related malnutrition/undernutrition without disease in adults. METHODS: The Malnutrition Universal Screening Tool (MUST) was used to assess malnutrition in this single-center, cross-sectional study. The malnourished group (n = 67) comprised patients with a MUST score of ≥ 2, and the control group (n = 31) included subjects with a MUST score of 0 - 1...
October 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/30336535/multianalyte-determination-of-noacs-using-lc-ms-ms-and-comparison-with-functional-coagulation-assays
#6
Ludek Slavik, Jiri Lukes, David Friedecky, Monika Zhanelova, Marketa Nemcova, Jana Ulehlova, Jana Prochazkova, Antonin Hlusi, Miroslava Palova, Jan Vaclavik
BACKGROUND: Detection of new oral anticoagulant (NOAC) levels by screening, special and global tests, and liquid chromatography-coupled tandem mass spectrometry (LC-MS/MS) is important in clinical situations when the cause of bleeding needs to be determined. METHODS: We compared a routine coagulation test, special function test for NOACs, global coagulation test, and an LC-MS/MS method that enables simultaneous determination of apixaban, dabigatran and rivaroxaban in human plasma within one analysis to determine the optimal indication of the comparison methods, including their limitations and interferences...
October 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/30336534/the-link-between-selenium-oxidative-stress-and-pregnancy-induced-hypertensive-disorders
#7
REVIEW
Teofana O Bizerea, Stefana G Dezsi, Otilia Marginean, Ramona Stroescu, Alexandru Rogobete, Otilia Bizerea-Spiridon, Constantin Ilie
BACKGROUND: The first line of defence against oxidative stress (OS) are the endogenous antioxidants, such as the Se containing compounds. During pregnancy, OS is caused by the intense growth activity of the fetus; therefore, the placenta is a key place for the activity of many seleno-compounds such as glutathione-peroxidase and thioredoxinreductase. METHODS: This review aims to establish the link between the type of selenium compounds, their concentration, their metabolic pathways, and their role in both physiologic and pathologic processes during pregnancy...
October 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/30336533/mir-33-may-be-a-biological-marker-for-coronary-heart-disease
#8
Zezhou Xie, Peipei Ma
BACKGROUND: The main aim of this study was to evaluate the expression and specific role of miR-33 in the progression of coronary heart disease (CAD), thereby evaluating their diagnostic ability and use in treatment in CAD patients. METHODS: Real time PCR was carried out to explore the level of miR-33 in the plasma of CAD patients and controls. ELISA was performed to analyze the level of placenta growth factor fragment (PLGF). Correlations between miR-33 and PLGF as well as other biochemical parameters were performed with Pearson's correlation analysis...
October 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/30336532/prognostic-value-of-alt-ast-and-aar-in-hepatocellular-carcinoma-with-b-type-hepatitis-associated-cirrhosis-after-radical-hepatectomy
#9
Li Zhou, Shao-Bin Wang, Shu-Guang Chen, Qiang Qu, Jing-An Rui
BACKGROUND: Alanine aminotransferase (ALT), aspartate aminotransferase (AST), and AST to ALT ratio (AAR) were shown to be associated with prognosis in some groups of hepatocellular carcinoma (HCC). However, their clinicopathologic and prognostic roles in HCC patients with B-type hepatitis-associated cirrhosis (HBAC) have not been comprehensively investigated. The present study aimed to address the issues. METHODS: A total of 125 patients with HCC and HBAC after radical hepatectomy were included...
October 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/30336531/a-case-report-of-46-xx-sex-reversal-syndrome
#10
Kangying Wang, Hua Lin, Haijian Tu, Qing Xu, Guanghui Xu
BACKGROUND: Sex reversal syndrome (SRS) is a human chromosomal abnormality disease with gender dysplasia, which is characterized by inconsistency between social sexuality and genetic sexuality. METHODS: We report a case of sex reversal syndrome with 46, XX. Chemiluminescence was used to detect serum sex hormones, including testosterone (T), luteinizing hormone (LH), and follicular stimulation (FSH), and 15 karyotype analysis. RESULTS: The levels of FSH and LH in serum were high, and the level of T in serum was low...
October 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/30336530/association-study-between-mtrr-taf4b-piwil1-variants-and-non-obstructive-azoospermia-in-northeast-chinese-han-population
#11
Xiao Yang, Hao Zhang, Yuting Jiang, Hongguo Zhang, Xiaonan Hu, Dongliang Zhu, Dongfeng Geng, Ruizhi Liu
BACKGROUND: Non-obstructive azoospermia (NOA) is an important factor leading to male infertility and the genetic mechanism is not yet clear. It requires investigation to reveal its occurrence based on sequencing technology from the genetic level. Our previous genome wide association study (GWAS) using targeted high-throughput sequencing technology has identified suspected genetic variants including rs162036, rs161870, rs1677016R and rs1106042R associated with non-obstructive azoospermia (data not published)...
October 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/30336529/five-fibrosis-biomarkers-together-with-serum-ferritin-level-to-diagnose-liver-fibrosis-and-cirrhosis
#12
Said M Afify, Ashraf Tabll, Hend M Nawara, Mohamed El Kassas, Ashraf Elfert, Masaharu Seno, Salah El-Kousy
BACKGROUND: Liver fibrosis is a dynamic procedure that results from an irregularity between fibrogenesis and fibrolysis. After time this procedure can lead to cirrhosis of the liver. Liver fibrosis and cirrhosis assessment is very important for both therapeutic decisions and prognostic evaluations. In this study, we tried to use serum ferritin (SF) together with five fibrosis tests (Age-Platelet index (API), aspartate aminotransferase to alanine aminotransferase ratio (AAR), AST to platelet ratio index (APRI), Fibrosis 4 score (FIB-4), and fibro-quotient (Fibro-Q)) to assess liver fibrosis and cirrhosis and estimate possible correlation between inflammation and SF...
October 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/30336528/study-on-the-correlation-between-urinary-transferrin-and-nonalcoholic-fatty-liver-disease
#13
Chuang Li, Weiwei Kong, Lixia Kang, Tiehan Zhang, Weiqun Zhang, Weidong Wang
BACKGROUND: Nonalcoholic fatty liver disease (NAFLD) can cause renal injury, and urinary transferrin (UTRF) is extremely sensitive to renal injury. We aimed to investigate the correlation between UTRF and NAFLD and to observe the distribution of NAFLD at different levels of UTRF. METHODS: A total of 711 subjects fulfilled the diagnostic criteria of NAFLD and 1,396 healthy control participants were enrolled in this study. UTRF levels and other clinical and laboratory parameters were measured...
October 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/30336527/polyparasitism-of-rhabditis-axei-and-enterobius-vermicularis-in-a-child-from-beijing-china
#14
Shuo Yang, Junxiong Wang, Dawei Shi, Liyan Cui, Rui Qiao, Shaogang Li
BACKGROUND: Rhabditis (Rhabditellae) axei is a common species in soil, which has been reported repeatedly in human urine and the digestive system. Humans exposed to sewage or mistakenly polluted sewage is the cause of larvae infecting the digestive tract or via the urethra. We reported a patient infected with Rhabditis axei and Enterobius Vermicularis. The migration of the nematodes caused true signs of hematuria, diarrhea, and high eosinophilia. METHODS: Stool and urine are collected to detect parasite eggs and genotype...
October 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/30336526/primary-cutaneous-nocardia-brasiliensis-in-a-spanish-child
#15
Maria Ercibengoa, Diego Vicente, Leonor Arranz, Aitor S de Ugarte, Jose M Marimon
BACKGROUND: We report a case of a primary cutaneous nocardiosis by autochthonous Nocardia brasiliensis in a Spanish immunocompetent 9-year-old boy. METHODS: N. brasiliensis caused cellulitis showing the patient recovery after drainage and treatment with trimethoprim-sulfamethoxazole. Nocardia grew in pure culture and its identification was confirmed by sequencing (16S rRNA) and by MALDI-TOF MS (Bruker, Daltonics, Germany). CONCLUSIONS: In Spain although N...
October 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/30336525/comparison-of-igg4-related-lymphadenopathy-and-multicentric-castleman-s-disease-a-retrospective-study
#16
Chuanyin Sun, Guanhua Xu, Jin Lin
BACKGROUND: It can be difficult to distinguish between IgG4-related lymphadenopathy and multicentric Castleman's disease (MCD) because these conditions cannot be differentially diagnosed using immunohistochemical staining alone. In this study, we analyzed the clinical features of IgG4-related lymphadenopathy and MCD patients. METHODS: We retrospectively analyzed 27 patients with MCD, including 20 with plasma cell-type (PC-type) and 7 with hyaline vascular (HV) features (mixed-type)...
October 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/30336524/serum-high-sensitivity-c-reactive-protein-improves-sensitivity-of-curb-65-in-predicting-icu-admission-and-mortality-in-community-acquired-pneumonia-patients
#17
Yan L Ge, Cong H Liu, Jing Xu, Zi Y Cui, Wei C Guo, Hui L Li, Ai S Fu, Hong Y Wang, Hai F Zhang, Xiao Y Zhu
BACKGROUND: The CURB-65 scoring system is a simple tool in assessment and prognosis prediction for communityacquired pneumonia (CAP) patients. However, the variations in performance of CURB-65 in young and elderly patients, underestimation or overestimation of the severity, and mortality have often been reported. The aim of this study was to investigate the usefulness of serum high-sensitivity C reactive protein (hs-CRP) combined with CURB-65 in predicting ICU admission and 30-day mortality in CAP patients...
October 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/30336523/mean-platelet-volume-shows-negative-correlation-with-anti-cyclic-citrullinated-peptide-antibodies-level
#18
LETTER
Jiyun Ryu, Anbok Lee, Sun Young Cho
No abstract text is available yet for this article.
October 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/30336522/rapidly-progressive-fata-infection-caused-by-bacillus-cereus-in-a-patient-with-acute-lymphoblastic-leukemia
#19
Jing-Wen Zhang, Yun-Xin Zeng, Jia-Yin Liang, Ling Zhang, Xin-Bao Zhao, Jian-Hua Pan, Ruo-Zhi Xiao
BACKGROUND: We herein report a fatal case of fulminant septicemia caused by Bacillus cereus in a 49-year-old female with T-cell acute lymphoblastic leukemia receiving chemotherapy. METHODS: Her two blood culture sets were positive for Gram-positive, rod-shaped bacterium. Bacillus cereus was identified by high-throughput MALDI-TOF mass spectrometry and 16S ribosomal RNA gene sequencing. RESULTS: The patient died within 12 hours from the onset of B cereus infection...
October 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/30336521/reduced-seroprevalence-of-hepatitis-a-virus-among-japanese-healthcare-workers-as-a-risk-factor-for-occupational-infection
#20
LETTER
Nori Yoshioka, Matsuo Deguchi, Hideharu Hagiya, Masanori Kagita, Kazunori Tomono
BACKGROUND: With the improvement in sanitation and hygiene, the incidence of hepatitis A virus (HAV) infection has declined, and its seroprevalence among Japanese people has been reduced. Universal HAV vaccination is yet to be implemented in Japan, and the healthcare workers (HCWs) are at higher risks of acquiring this infection. We herein report the seroepidemiology of HAV infection among HCWs at Osaka University Hospital. METHODS: Between September and October 2017, we collected serum samples submitted to our laboratory for HCWs health examination and tested for the anti-HAV antibody...
October 1, 2018: Clinical Laboratory
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