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Clinical Laboratory

Wen-Xing Li, Fei Cheng, A-Jie Zhang, Shao-Xing Dai, Gong-Hua Li, Wen-Wen Lv, Tao Zhou, Qiang Zhang, Hong Zhang, Tao Zhang, Fang Liu, Dahai Liu, Jing-Fei Huang
BACKGROUND: Hyperhomocysteinemia (HHcy) is an independent risk factor for cardiovascular diseases (CVDs). We aimed to investigate the joint effect of homocysteine metabolism gene polymorphisms, as well as the folate deficiency on the risk of HHcy in a Chinese hypertensive population. METHODS: This study enrolled 480 hypertensive patients aged 28 - 75 from six hospitals in different Chinese regions from 9/2005 - 12/2005. Known genotypes of methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C, methionine synthase (MTR) A2756G, and methionine synthase reductase (MTRR) A66G were detected by PCRRFLP methods...
March 1, 2017: Clinical Laboratory
Ilaria Crespi, Maria Giovanna Sulas, Riccardo Mora, Paola Naldi, Domizia Vecchio, Cristoforo Comi, Roberto Cantello, Giorgio Bellomo
BACKGROUND: Isoelectrofocusing (IEF) to detect oligoclonal bands (OBCs) in cerebrospinal fluid (CSF) is the gold standard approach for evaluating intrathecal immunoglobulin synthesis in multiple sclerosis (MS) but the kappa free light chain index (KFLCi) is emerging as an alternative marker, and the combined/sequential uses of IEF and KFLCi have never been challenged. METHODS: CSF and serum albumin, IgG, kFLC and lFLC were measured by nephelometry; albumin, IgG and kFLC quotients as well as Link and kFLC indexes were calculated; OCBs were evaluated by immunofixation...
March 1, 2017: Clinical Laboratory
Hyung-Seok Yang, Sun Young Cho
No abstract text is available yet for this article.
March 1, 2017: Clinical Laboratory
Fatma Erdem, Ayham Abulaila, Zerrin Aktas, Oral Oncul
BACKGROUND: Rapid, simple, and accurate laboratory detection of carbapenemases is very important for proper antibiotic therapy and infection control. METHODS: In this study, carbapenem-nonsusceptible Enterobacteriaceae (CRE) isolates were used to evaluate the performance of a new lateral flow immunochromatographic (IC) assay, the OXA-48 and KPC K-SeT assay, and modified Blue-Carba test (BCT) for the rapid detection of OXA-48 carbapenemase in comparison with polymerase chain reaction (PCR) amplification...
March 1, 2017: Clinical Laboratory
Junxiao Lin, Biao Ma, Jiehong Fang, Ye Wang, Haizhen He, Wei Lin, Wei Su, Mingzhou Zhang
BACKGROUND: Human papillomavirus (HPV) infection is linked to cervical cancer. With the technological development of molecular biology and epidemiology, detection and treatment of HPV has become an important mean to prevent cervical cancer. METHODS: A simple, rapid, and sensitive colorimetric loop-mediated isothermal amplification (LAMP) method was established herein to detect 23 HPV genotypes. The sequences of the primers for the LAMP reaction were located in the L1 gene of the HPV genome...
March 1, 2017: Clinical Laboratory
Xi Chen, Peiyan Zheng, Nili Wei, Huimin Huang, Wenting Luo, Yongping Lin, Baoqing Sun
BACKGROUND: Various new methods to diagnose allergens have gradually developed in recent years. The present study aimed to evaluate the diagnostic performance and clinical utility of Allergy-Q detection system (Proteometech Inc., Korea) on serum allergen-specific IgE (sIgE). METHODS: Immunoblotting (Allergy-Q system) and fluorescent enzyme immunoassay (ImmunoCAP system, Phadia Inc., Sweden) were used to detect serum total IgE and allergen sIgE of 244 cases of allergic diseases, followed by comparative analysis of the results...
March 1, 2017: Clinical Laboratory
Amira Moussa, Sonia Triki, Haithem Hamdouni, Ons Fekih, Marwa Ajmi, Ibtihel B hajMbarek, Afifa Koubaa, Fadoua Neffati, Asma Omezzine, Med-Fadhel Najjar, Ali Bouslama
BACKGROUND: The aim of this study was to evaluate the association of ACE, angiotensinogen (AGT) and angiotensin II receptor type I (AGTR1) polymorphisms with diabetic nephropathy (DN) in Tunisians. METHODS: The study population comprised 236 type 2 diabetic patients: with nephropathy (DN = 47) and without nephropathy (DM = 189). Genotyping of ACE-I/D-rs1799752, ACE-rs4343G>A, AGT-rs5050A>C, AGT-rs 4762C>T, AGT-rs699A>G, and AGTR1-rs5186A>C was performed by PCR-RFLP...
March 1, 2017: Clinical Laboratory
Min He, Pei-Feng Ke, Wei-Ye Chen, Hai-Biao Lin, Yun-Xiu Wang, Ze-Min Wan, Xin-Zhong Wu, Jun-Hua Zhuang, Xian-Zhang Huang
BACKGROUND: Cystatin C (CysC) is an endogenous filtration marker for estimation of kidney function. This study aimed to define the reference interval (RI) for serum CysC in a southeast Chinese adult population and to explore the variables that affect serum CysC levels. METHODS: 532 reference individuals (259 male, 273 female, aged 18 - 79 years) were recruited from Guangzhou, China. Multiple regression analysis (MRA) was used to investigate the association between serum CysC levels and clinical factors including age, gender, body mass index, lifestyle, and biochemistry parameters...
March 1, 2017: Clinical Laboratory
Tiejun Chen, Liping Yu, Bin Li, Fujuan Zhang, Yue Wang, Xi Wang, Sha Liu, Chunjiao Yang, Hui Qi, Meiling Yu, Shuying Meng, Tao Chen
BACKGROUND: We investigated the prognostic significance of carcinoembryonic antigen (CEA) and vascular endothelial growth factor (VEGF) and their associations with clinicopathological features in patients with sigmoid colon carcinoma (SCC). METHODS: We retrospectively reviewed patients with SCC treated with curative surgery from January 2001 to June 2010 at our hospital. Patients' general, clinical, histopathologic, and serum biomarkers were analyzed. We measured VEGF in 176 sets of SCC tissues and adjacent noncancerous tissue samples with immunohistochemical and Elivison staining...
March 1, 2017: Clinical Laboratory
Suekyeung Kim, Sun Young Cho, Hyung-Seok Yang
No abstract text is available yet for this article.
March 1, 2017: Clinical Laboratory
Wenhai Huang, Shuoyun Wu, Zhichao Lin, Peisong Chen, Guoyong Wu
BACKGROUND: The aim of our study was to evaluate the performance of HE4 in the diagnosis and follow up in patients with non-small cell lung cancer (NSCLC). METHODS: Serum levels of HE4, CEA, and Cyfra 21-1 were analyzed in 146 patients suspected with NSCLC and 30 healthy subjects to evaluate their diagnostic performance. A one year follow up was performed in 61 patients confirmed with NSCLC after surgery eradication at the interval of 1 month, 3 months, 6 months, and 12 months...
March 1, 2017: Clinical Laboratory
Jun-Chi Xu, Ming Li, Yuan Ma, Cui-Lin Shi, Xiao-Yan Zhu, Hua-Feng Song, Hui Chen, Chuan-Wu Zhu, Ping Xu
BACKGROUND: B7-H3 is frequently upregulated in response to autoantigens and pathogens during host T cell immune responses. However, the role of B7-H3 which express in CD14+ monocytes and CD4+CD25high T cells had not been investigated. METHODS: Cytometry and ELISA were used in this study. RESULTS: The study showed that B7-H3 expression in CD14+ monocytes, CD4+CD25high T cells, and plasma was significantly increased in AHB, CHB, HBV-LC, and HBV-HCC group...
March 1, 2017: Clinical Laboratory
Reham M Dawood, Eman M Mahmoud, Marwa K Ibrahim, Noha G Bader El Din, Ahmed Aboul-Enein, Naglaa Zayed, Yasmine S El Abd, Hadeel G Eldeen, Rasha Eletreby, Marwa Elsharkawy, Moataza Omran, Reem El-Shenawy, Sayed A Fayed, Mostafa K El Awady
BACKGROUND: Methylene tetrahydrofolate reductase (MTHFR) C677T polymorphism was reported as a genetic variant in liver steatosis and fibrosis. This is a study of the association between MTHFR C677T polymorphism and HCV core with severity of steatosis in HCV GT4 patients. METHODS: 111 HCV patients and 112 control subjects were recruited. Polymorphism was detected by RFLP analysis, core Ag was detected by ELISA. RESULTS: Combined HCV infection and MTHFR C677T polymorphism increases the risk to develop steatosis by 3...
March 1, 2017: Clinical Laboratory
Jiubo Fan, Ji Ma, Ning Xia, Li Sun, Baoan Li, Haiju Liu
BACKGROUND: Acute myocardial infarction (AMI) is a serious life-threatening and common heart disease that is based on coronary atherosclerosis. The aim is to study the changes in the level of kinase isoenzyme (CK-MB), myoglobin (MYO), cardiac troponin I (cTnI), and plasma N-terminal pro-B-type natriuretic peptide (NT-proBNP) in patients with acute myocardial infarction (AMI) and the four indicators of joint detection clinical value for diagnosis of AMI. METHODS: Plasma NT-proBNP, CK-MB, MYO, and cTnI were detected by CLIA in 208 AMI patients (AMI group) and 115 non-AMI patients (control group)...
March 1, 2017: Clinical Laboratory
Zhang Xiao-Lei, Deng Fang, Shan Wu-Lin, Ge Xin, Li Ming
BACKGROUND: Fungal spores can be observed on peripheral blood smears and may affect cell counting in an automated hematology counter. The purpose of this study was to evaluate the effects of fungal spores on cell counting in Sysmex-5000i automated hematology counter. METHODS: The common clinical fungal spores we selected were C. albicans, C. krusei, C. tropicalis, and C. glabrata, using 0.9% normal saline to formulate a final concentration of 4 - 6 x 109/L fungal liquid, mixed with peripheral blood (containing high, medium and low concentrations of white blood cells and platelets), and formulated to different concentrations (4 - 6 x 108/L, 4 - 6 x 107/L, 4 - 6 x 106/L, 4 - 6 x 105/L)...
March 1, 2017: Clinical Laboratory
Yao Wang, Yuanting Li, Zhiyong Chen, Tianhao Wang, Jie Gu, Xianhua Wu, Yi Yin, Maosheng Wang, Zhigang Pan
BACKGROUND: DESMIN is a novel prognostic predictor and therapeutic target for colorectal cancer (CRC). Enzyme-linked immunosorbent assay (ELISA) and electrochemiluminescence (ELC) assays are large-scale and highcost projects; therefore, it is necessary to develop a new, fast, and simple yet highly sensitive and specific method to detect DESMIN in serum. Semiconducting quantum dots (QDs) possess high fluorescence quantum yield, stability against photobleaching, and size-controlled luminescence properties, thus being utilized in photoelectrochemical tumor marker detection, especially in ameliorating the diagnostic value in complex biological ambient ionization...
March 1, 2017: Clinical Laboratory
Zhinan Liu, Jingsheng Cai, Yue Yu, Haisheng Fang, Yan Si, Jinista J Jankee, Meiping Shen
BACKGROUND: High level of serum tumor abnormal protein (TAP) may be useful in early diagnosis of cancers. This study aims to investigate the diagnostic value of TAP in patients with papillary thyroid carcinoma (PTC). METHODS: Abnormal sugar chain glycoproteins from peripheral blood of 139 patients with PTC, 39 patients with benign thyroid nodular and 35 healthy individuals were used. Receiver operating characteristic (ROC) curve was performed to evaluate diagnostic value of tumor abnormal protein (TAP)...
March 1, 2017: Clinical Laboratory
E Sonmez, A Dursun, B Gulen, H Metin, O F Ozer
BACKGROUND: SCUBE1 has recently been studied as a diagnostic biomarker for acute coronary syndrome, ischemic stroke, and acute mesenteric ischemia. The aim of this study is to evaluate the value of SCUBE1 and routine parameters used in patients diagnosed with acute appendicitis. METHODS: Of the 150 patients admitted to the emergency department whose initial diagnosis were acute appendicitis (AA), 103 patients were excluded from the study for various reasons. Forty-seven patients with a definitive diagnose of AA and 43 volunteers were enrolled in the study...
March 1, 2017: Clinical Laboratory
Afsaneh Labbaf, Hamid Ghaedi, Behnam Alipoor, Mir Davood Omrani, Faranak Kazerouni, Mehrnoush Shanaki, Maryam Ghaffarzadeh, Hossein Pashaiefar, Ali Rahimipour
BACKGROUND: Recent reports have suggested that single-nucleotide polymorphisms (SNPs) in microRNA genes may contribute to individual susceptibility to coronary artery disease (CAD). The purpose of this study was to evaluate the association between rs3746444 in pre-miR-499 with CAD. METHODS: We performed a case-control study including 288 CAD patients and 150 control subjects. DNA was extracted from blood samples and genotyped through the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method...
March 1, 2017: Clinical Laboratory
Lianli Yin, Xiang Chen, Yinghua Tang, Yifan Sun
BACKGROUND: Progesterone is a reliable indicator of either natural or induced ovulation, and it plays an important role in preparing for implantation in the uterus and maintaining pregnancy. Estradiol is the most powerful natural estrogen in humans. It adjusts reproductive function in females and, with progesterone, maintains a pregnancy. Prolactin is also an important indicator, and its major physiological action is the initiation and maintenance of lactation in women. Architect i2000sr and Cobas e601 are automated immunoassay systems that are widely used to measure progesterone, estradiol, and prolactin concentrations in the blood...
March 1, 2017: Clinical Laboratory
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