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Clinical Laboratory

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https://www.readbyqxmd.com/read/29226659/performance-evaluation-of-the-bio-rad-d-100-system-for-hemoglobin-a1c-assay
#1
Tae-Dong Jeong, Sholhui Park, Ki-Sook Hong
No abstract text is available yet for this article.
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29226658/application-of-commutable-erm-da474-ifcc-for-harmonization-of-c-reactive-protein-measurement-using-five-analytical-assays
#2
Chunying Wu, Shunli Zhang, Wei Liu, Jie Zeng, Tingting Zhao, Yuhong Yue, Rui Zhang, Huaian Ma, Qingtao Wang
BACKGROUND: ERM-DA474/IFCC has been used as a reference material for C-reactive protein (CRP) since 2012. However, the commutabilities and the capacity for harmonizing CRP results of the material and its dilutions have not yet been reported. In this study, we aimed to evaluate the harmonization of CRP results using commutable ERM-DA474/IFCC. METHODS: Twenty-one serum samples were collected and split into five vials. The samples were then analyzed using five popular assays (Siemens BN II, Beckman Immage 800, Roche, Diasys, and Leadman)...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29226657/short-centrifugation-to-ameliorate-turn-around-time-in-routine-coagulation-testing
#3
Albrecht Pfaefflin, Karen Schuster, Ruediger Braun
BACKGROUND: Fast and timely analysis of coagulation parameters is mandatory in hospital laboratories as the results are decisive for diagnosis and treatment in emergency patients. An important factor in TAT (turn-around time) prolongation is centrifugation of all samples prior to analysis. According to literature and most current SOPs (standard operation protocols) centrifugation time is given with 10 minutes at approximately 2,000 g and cannot be reduced without infringement of standards...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29226656/peripheral-blood-mir-181-5p-serves-as-a-marker-for-screening-patients-with-osteoarthritis-by-targeting-tnf%C3%AE
#4
Silong Xia, Hua Tian, Lei Fan, Jianying Zheng
BACKGROUND: Increasing evidence has suggested the important role of miRNAs in the progression of osteoarthritis (OA). In the current study, we explored the role of miR-181-5p in the peripheral blood and bone tissues of OA patients for the first time. METHODS: The level of miR-181-5p was evaluated in the peripheral blood and cartilage of OA patients using real time PCR analysis. ROC analysis was performed to explore whether peripheral blood and cartilage miR-181-5p could screen OA patients from normal controls...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29226655/increased-expression-of-pcna-as1-in-colorectal-cancer-and-its-clinical-association
#5
Fengbo Cai, Juntang Li, Xuefeng Pan, Changshan Zhang, Dong Wei, Chunfang Gao
BACKGROUND: The aim is to study the expression of PCNA-AS1 in colorectal cancer (CRC) tissue and paired-adjacent normal tissue and the relationship between its expression level and clinical pathological features. METHODS: Using real-time qPCR, PCNA-AS1 expression levels were detected in 114 cases to establish the relationship between its expression and clinicopathologic features. Moreover, the expressions of PCNA-AS1 were investigated in CRC and normal colonic epithelial cell lines...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29226654/evaluation-of-hepcidin-and-atherosclerosis-in-dialysis-patients
#6
Victor Manolov, Julia Petrova, Boris Bogov, Savina Hadjidekova, Vasil Vasilev, Dyana Yonova, Maria Petrova, Todor Kunchev, Yavor Jelev, Petar Jeliazkov, Borislav Marinov, Ivo Bogov, Zlatina Gramatikova, Silviya Voleva, Kamen Tzatchev, Latchezar Traykov
BACKGROUND: Chronic kidney disease (CKD) is a condition that involves 10% - 15% of population worldwide, which increases the risk of cardio-vascular diseases (CVD). Chronic kidney disease is one of the main reasons for illness and mortality in the world. Chronic kidney disease is a serious health problem caused by involvement of a large number of patients with kidney injury, especially in industrial countries. Among the main reasons for this are population living longer and the number of diseases in elderly persons, such as diabetes mellitus type 2, hypertension, and cardio-vascular diseases...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29226653/a-preliminary-study-of-the-value-of-plasma-microrna-193b-and-soluble-urokinase-type-plasminogen-activator-receptor-in-identifying-patients-with-early-stage-colorectal-cancer-and
#7
K L Liu, J L Luo, J Wu, Y D Wang, H J Fan
BACKGROUND: Early detection and management of colorectal cancer (CRC) and colorectal adenoma (CRA) reduces the mortality and morbidity of CRC, but there is a lack of ideal circulation biomarkers. METHODS: A total of 80 patients with early-stage CRC and CRA and 30 healthy controls were included in this preliminary study. Plasma samples were collected before colonoscopy and prepared for measurement of microRNA193b and soluble uPAR. RESULTS: Plasma level of miR-193b was decreased through the normal-adenoma-carcinoma sequence with no significant difference between patients with CRC and advanced CRA...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29226652/a-pooled-analysis-of-the-clinical-utilities-of-long-non-coding-rna-based-molecular-signature-for-diffuse-large-b-cell-lymphoma
#8
Pei Xu, Xiaoli Chen, Panke Su
BACKGROUND: Long non-coding RNAs (lncRNAs) have been highlighted as promising biomarkers for several types of malignancies. The current meta-analysis sought to investigate the clinical value of lncRNA-based molecular signatures as biomarkers for diagnosis, subtype classification, and prognosis in diffuse large B cell lymphoma (DLBCL). METHODS: A systematic search of the online databases was carried out and data were retrieved by pairs of independent reviewers. Effect sizes of the diagnostic parameters were combined using a quantitative meta-analysis...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29226651/quantitative-distinction-of-morphological-characteristics-of-white-blood-cells-in-peripheral-blood-through-texture-analysis-using-gray-level-co-occurrence-matrix
#9
Mai Yamamoto, Keigo Kono, Maiko Kuroki, Satoru Murakami, Ruka Hayata, Kana Nanato, Minrong Shao, Keiko Miwa, Yutaka Tsutsumi, Kazunori Okada, Sanae Kaga, Nobuo Masauzi
BACKGROUND: Texture features are valuable clues for skilled technicians to differentiate peripheral blood (PB) white blood cells (WBCs). Some studies have tried to distinguish WBCs automatically by using texture analysis. However, no study so far has applied a gray level co-occurrence matrix (GLCM) to images of PB WBCs. Here, we developed a new GLCM method, called the CM-Tx method, for automatically distinguishing PB WBCs. METHODS: We used a total of 199 images of six different types of PB WBCs, taken from PB smears of 12 healthy volunteers, as objective standard images for the analysis...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29226650/multiple-nonleukemic-myeloid-sarcoma-associated-hemophagocytic-lymphohistiocytosis-in-an-adult
#10
Jingwen Zhang, Yunxin Zeng, Xiangzhong Zhang, Wenxing Lai, Yuxin Chen, Ruozhi Xiao
BACKGROUND: Nonleukemic myeloid sarcoma (MS) occurs rarely. Hemophagocytic lymphohistiocytosis (HLH) is a rare and potentially fatal condition. We report a rare case of nonleukemic MS associated with HLH. METHODS: Hematologic investigation, 18F-FDG PET/CT, bone marrow aspirate and biopsy, and lymph node biopsy were performed in a 25-year-old male patient. RESULTS: The patient was given a short-term treatment of etoposide and dexamethasone to control HLH...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29226649/comparison-of-the-diagnostic-power-of-serum-il-6-il-8-and-tnf-%C3%AE-for-the-idiopathic-anterior-uveitis-in-children
#11
Beata Zelazowska-Rutkowska, Malgorzata Mrugacz, Bogdan Cylwik
BACKGROUND: Uveitis is the inflammation of the uvea that often occurs in children. There are many causes of disease, but some of them do not have any reasons and are then called idiopathic uveitis. Cytokines play an important role in the regulation of the immune response. Determination of cytokine profiles could contribute to the explanation of the etiology of uveitis and could serve to evaluate the inflammation intensity as well as be helpful in the early diagnosis this disease. The purpose of this study was to determine the serum level of selected inflammatory cytokines, such as interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor α (TNF-α) and to compare their diagnostic power as a markers of idiopathic anterior uveitis in children...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29226648/fibulin-levels-in-autosomal-dominant-polycystic-kidney-disease-and-its-relationship-to-arterial-stiffness
#12
A Inci, O Akin, H Y Ellidag, E Eren, S Dolu, F Sari
BACKGROUND: The objective of the present study was to evaluate fibulin 1 levels in different stages of patients with autosomal dominant polycystic kidney disease (ADPKD) and investigate possible connections between fibulin-1 and arterial stiffness. METHODS: For this cross-sectional study, we included 74 patients with ADPKD (mean age, 50.92 ± 15.70 years) and 32 healthy controls (mean age, 49.53 ± 7.32 years). Patients with ADPKD were classified based on CKD epidemiology collaboration (CKD-EPI) equation assessments of estimated glomerular filtration rate (eGFR)...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29226647/human-peripheral-blood-cells-mrna-levels-are-highly-sensitive-to-duration-of-ex-vivo-post-sampling-conditions-prior-to-rna-isolation
#13
Eva Bohackova, Pavlina Dankova
BACKGROUND: This study aimed to evaluate an effect of the time period between drawing the peripheral blood and specimen processing on the stability of mRNA levels of 7 selected genes. METHODS: Blood samples derived from 15 healthy volunteers were always processed at five consecutive time points 0.5, 1.5, 2, 3, and 9 hours; mRNA was quantified by real-time PCR. RESULTS: Anti-inflammatory genes CCL2 and IL10 showed a significant rise of expression between the 3rd and 9th hour after blood collection (p ≤ 0...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29226646/comparison-of-citrate-buffer-with-sodium-fluoride-as-additives-in-determining-glycemia
#14
Maria de Toro-Crespo, Jose M Maesa-Marquez, Victor Sanchez-Margalet, Concepcion Gonzalez-Rodriguez, Patricia Fernandez-Riejos
BACKGROUND: The objectives of this study are to compare the effect of sodium fluoride and citrate on the stability of glucose in samples maintained at room temperature up to three hours, and to assess the clinical impact in the O'Sullivan test results after changing the additives in the collecting tubes. METHODS: The selected population was pregnant women between the 24th and 28th week of gestation, who were at the health center to undergo the O'Sullivan test as part of the screening program for GDM (gestacional diabetes mellitus)...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29226645/thrombophilic-mutations-among-patients-with-sickle-cell-disease
#15
Rim B Nefissi, Faida Ouali, Taieb Massaoud, Nasreddine Gritli
BACKGROUND: Factor V-Leiden (FVL), Prothrombin (PRT) G20210A, and Methylene Tetrahydro Folate Reductase (MTHFR) C677T and A1298C mutations are major inherited risk factors of thrombotic complications. Our aim in this study was to investigate the prevalence of these mutations among Tunisian sickle cell patients. METHODS: Study subjects comprised 64 patients and 100 healthy controls. FVL, PRT G20210A, and MTHFR genotypes were determined using a reverse dot blot based method...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29226644/the-role-of-microrna-1207-5p-in-colorectal-cancer
#16
Xiaokun Wang, Xiaodong Wu
BACKGROUND: MicroRNA-1207-5p (MiR-1207-5p) has been identified as a tumor suppressor in many types of cancer. However, less research has been done with respect to the role of miR-1207-5p in colorectal cancer (CRC). The aim of our study was to analyze the role of miR-1207-5p in CRC. METHODS: Total RNA was extracted from clinical specimens and CRC cell line, and the expression of miR-1207-5p was examined by real-time PCR (q-PCR). The human CRC cell lines were transfected with the miR-1207-5p mimic, negative control (NC)...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29226643/prognostic-value-of-serum-lactate-dehydrogenase-in-patients-with-nasopharyngeal-carcinoma-a-meta-analysis
#17
Changyun Zhai, Ke Gu, Xiaoming Zhai, Jianping Wang, Junning Zhang
BACKGROUND: Serum-lactate dehydrogenase (S-LDH) is reported to be associated with poor survival in patients with nasopharyngeal carcinoma (NPC); however, the results are inconsistent. The aim of the study was to perform a meta-analysis to evaluate the prognostic value of S-LDH in patients with NPC. METHODS: PubMed and Web of Science were searched for relevant studies, and the fixed-effects model was employed to pool the hazard risks (HRs) from individual studies when no substantial heterogeneity was detected; otherwise, the random-effects model was used...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29226642/homoarginine-associates-with-zonulin-and-tryptophan-findings-in-a-high-risk-cohort-of-patients-carrying-an-aicd
#18
Elli Mann, Robert Kluesener, Felix Boschann, Jana Ruppert, Franz P Armbruster, Andreas Meinitzer, Christoph Melzer, Thomas B Dschietzig
BACKGROUND: Homoarginine (hArg) is known to have an impact on nitric oxide (NO) metabolism. It seems to increase NO generation and/or availability, thereby enhancing endothelial function. In addition, hArg is connected to energy metabolism since the key enzyme, L-arginine-glycine amidinotransferase (AGAT) for hArg synthesis in the kidneys, is also involved in the synthesis of energy metabolites like guanidinoacetate. Former studies indicate that low levels of hArg are linked to cardiovascular disease and increased all-cause mortality...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29226641/modified-complete-blood-count-indices-as-predicting-markers-of-preeclampsia-from-gestational-hypertension-neutrophil-to-lymphocyte-ratio-platelet-to-lymphocyte-ratio-and
#19
You La Jeon, Woo-In Lee, So Young Kang, Myeong Hee Kim
BACKGROUND: To evaluate modified complete blood count (CBC) indices as a predicting marker of preeclampsia (PE) from gestational hypertension (GH), we analyzed the neutrophil to lymphocyte ratio (NLR), platelet to lymphocyte ratio (PLR), and platelet to neutrophil ratio (PNR). PNR was a newly designed index in this study based on results of PE patients having a tendency toward higher neutrophil count and lower platelet count compared to normal pregnant women in previous studies. METHODS: We recruited 86 normal pregnant women, 33 patients with GH and 68 patients with PE...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29226640/association-of-paraoxonase-1-polymorphism-and-serum-25-hydroxyvitamin-d-with-the-risk-of-cardiovascular-disease-in-patients-with-rheumatoid-arthritis
#20
Sawsan O Khoja, Yasser El Miedany, Archana P Iyer, Sami M Bahlas, Khadijah S Balamash, Mohamed F Elshal
BACKGROUND: Patients with rheumatoid arthritis (RA) have significantly increased cardiovascular (CV) morbidity and mortality that are not accounted for by traditional risk factors alone. Paraoxonase 1 (PON1) and 25-hydroxyvitamin D have been shown to be involved in the pathogenesis of CV diseases. Objective: This study aimed to investigate PON1 gene polymorphism and serum 25-hydroxyvitamin D concentrations in RA patients, and to determine their association with CV risk in RA. METHODS: Serum samples from 46 RA patients and 45 healthy controls were tested for PON1 R192Q genotypes and serum vitamin D concentrations...
November 1, 2017: Clinical Laboratory
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