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Clinical Laboratory

Xiao Hu, Bin He, Lin Zhou, Haiyang Xie, Shusen Zheng
BACKGROUND: To investigate the expression pattern of Gab2 in hepatocellular carcinoma (HCC) and explore the correlation between Gab2 expression and clinicopathological features of HCC patients. The prognostic significance of Gab2 expression is evaluated to determine the possible role in the progression of HCC. METHODS: Gab2 expression was detected by immunohistochemistry in 90 HCC samples and matched adjacent noncancerous liver tissues. The mRNA and protein of Gab2 in HCC and normal liver cell lines were examined by quantitative RT-PCR and western blot, respectively...
2016: Clinical Laboratory
Nermin Karakurt, Levent Filik
No abstract text is available yet for this article.
2016: Clinical Laboratory
Young Jin Kim, Hyung-Seok Yang, Tae Sang Oh, Min Jin Kim, Hee Joo Lee
No abstract text is available yet for this article.
2016: Clinical Laboratory
Radu Minciu, Raluca Dumache, Pupca Gheorghe, Livius Daminescu, Alexandru F Rogobete, Daniela Ionescu
BACKGROUND: Worldwide prostate cancer (PCa) represents the 2nd leading cause of cancer related deaths among men. Currently, the screening for early detection of PCa is based on determination of serum prostate-specific antigen (PSA) levels. But this biomarker presents some disadvantages related to its specificity and sensitivity. In our study, we want to determine if methylation levels of the glutathione S-transferase P1 (GSTP1) gene could be used as a new biomarker for the early detection of PCa and to distinguish between malignant and benign pros-tatic lesions...
2016: Clinical Laboratory
Xiaoran Duan, Chunyang Li, Tuanwei Wang, Xiaolei Feng, Liuxin Cui, Wu Yao, Wei Wang
BACKGROUND: In recent research, it has been shown that there have been variants of rs894160 within the PLINI gene which have been associated with obesity, type 2 diabetes, and other diseases. But the isoschizomers such as the Mn1I enzyme required for the detection of this polymorphism are expensive. METHODS: The study used an improved PCR-RFLP method with mismatched base for detection of the single nucleotide polymorphism rs894160. RESULTS: After detecting 550 Chinese Han individuals, the genotype frequencies were 26...
2016: Clinical Laboratory
Niels Straub, Ekaterina Bauer, Seema Agarwal, Patrick Meybohm, Kai Zacharowski, Alexander A Hanke, Christian F Weber
BACKGROUND: The economic effects of Point-of-Care (POC) coagulation testing including Multiple Electrode Aggregometry (MEA) with the Multiplate device have not been examined. METHODS: A health economic model with associated clinical endpoints was developed to calculate the effectiveness and estimated costs of coagulation analyses based on standard laboratory testing (SLT) or POC testing offering the possibility to assess platelet dysfunction using aggregometric measures...
2016: Clinical Laboratory
Yan Chen, Xiaoqin Xin, Zhaolei Cui, Yuhong Zheng, Junying Guo, Yansong Chen, Yingying Lin, Guangjian Su
BACKGROUND: Epstein-Barr virus capsid antigen immunoglobulin A (EBV VCA-IgA) exerts an important role in the diagnosis of nasopharyngeal carcinoma (NPC). This meta-analysis aimed to evaluate the pooled diagnostic performance of VCA-IgA for NPC. METHODS: Literature fulfilling the criteria was searched in PubMed and Embase databases. The quality of the studies was assessed in terms of the Quality Assessment of Diagnosis Accuracy Studies (QUADAS) criteria. The pooled diagnostic parameters were generated using a bivariate meta-analysis model...
2016: Clinical Laboratory
Jun Cai, Zhongtao Zhang
BACKGROUND: Patients with chronic inflammatory bowel disease (IBD) are at high risk of developing colon cancer and represent a valuable patient cohort for studying the correlation between chronic inflammation and cancer formation. Cytokines play key roles in the regulation of systemic inflammation, local tissue damage, and immunomodulation involved in tumor development and progression. Therefore, functional polymorphisms in genes that encode cytokines and cytokine receptors represent potential candidate genes associated with carcinogenesis...
2016: Clinical Laboratory
Fengfeng Zhang, Binbin Wang, Houlong Long, Jingkui Yu, Feng Li, Haitao Hou, Qifeng Yang
BACKGROUND: Recent findings have revealed that abnormal expression of microRNAs (miRNA, miR) contributes to the malignancies of various cancers. Here, we report a novel miRNA that regulates the expression of Beclin-1 in breast cancer cells. METHODS: The expression of miR-124-3p and Beclin-1 was identified in breast cancer tissues and breast cancer cell lines. To explore whether Beclin-1 was the target gene of miR-124-3p, luciferase reporter assay was applied. MIR-124-3p was overexpressed or inhibited with the corresponding mimics or inhibitors...
2016: Clinical Laboratory
Liangcai Xie, Wenli Cheng, Lei Xu, Wen Fan, Yirong Li
BACKGROUND: Rheumatoid factor (RF) can interfere both positively and negatively in immunoassays. It remains unclear whether the negative interference is an exceptional phenomenon or a denominator of immunoassays and which RF subgroup plays a key role in its causation. METHODS: Serum models comprising hepatitis B envelope antigen (HBeAg) and RF were made by blending HBeAg-positive sera and RF-positive sera at a ratio of 1:9. Paramagnetic microparticles coated with P human chorionic gonadotropin (betaHCG)-anti-betaHCG complexes were used to remove RFs from the models, and HBeAg was determined in models using the chemiluminescent microparticle immunoassay (CMIA)...
2016: Clinical Laboratory
Mohamed M Abdel-Aziz, Mohamed F Elshal, Aymn T Abass, Sherif El-Kafrawy, Sameera Ezzat, Mohamed Abdel-Wahab
BACKGROUND: Hepatocellular carcinoma (HCC) has the worst prognosis among all major cancers, largely due to the lack of sensitive diagnostic markers. We aimed to compare three HCC tumor markers, alpha-fetoprotein (AFP), p53, and AFP-L3%, to evaluate whether measuring serum p53 levels and AFP-L3% has an additive diagnostic value for detection of HCC. METHODS: A total of 86 patients with chronic liver diseases were included. HCC was detected in 68 (79.1%) patients...
2016: Clinical Laboratory
Mingmei Huangfu, Linlin Liu, Shuang Xu, Siyao Li, Kuo Jiang, Baosheng Sun, Kuang-Hui Lee, Shilong Sun
BACKGROUND: Over-expression of tumor-associated antigens (TAAs) may trigger secretion of their auto-antibodies. The present work was designed to test whether circulating antibody to P16 protein-derived antigens was altered in cervical cancer. METHODS: 141 cases of cervical cancer patients, 133 cases of cervical benign tumor patients, and 153 healthy volunteers matched in age were recruited. The level of circulating P16 auto-antibody was tested using an ELISA developed in-house with linear peptide antigens derived from the P16 protein...
2016: Clinical Laboratory
Paul Bălănescu, Anca Lădaru, Eugenia Bălănescu, Valer Pompilian, Daniela Gologanu, Simona Caraiola, Cristian Băicuş, Gheorghe A Dan
BACKGROUND: Proteomic candidate biomarkers for systemic sclerosis (Ssc) useful for appropriate patient evaluation and follow-up were identified in mass-spectrometry studies; however, most of these biomarkers were not evaluated and confirmed on independent patient samples. Up-regulation of reticulocalbin 1 (RCN1) and reticulocalbin 3 (RCN3) in the dermal fibroblast secretome originating from Ssc patients was previously described. The aim of the study was to evaluate circulating RCN1 and RCN3 as candidate biomarkers for Ssc clinical expression...
2016: Clinical Laboratory
Guilian Cheng, Nian Zhang, Yueqin Wang, Jiang Rui, Xiong Yin, Tianpen Cui
BACKGROUND: Recently, autoimmune mechanisms and pulmonary epithelial cells have attracted attention in chronic obstructive pulmonary disease (COPD). Circulating antibodies against human bronchial epithelial cells (anti-HBEC) bind to bronchial epithelial antigens and induce bronchial epithelial cell damage. This study aimed to detect the expression of IgG, IgM, and IgA anti-HBEC in patients with COPD. METHODS: The association of gender, age, body mass index (BMI), and pulmonary function with the presence of IgG, IgA, and IgM anti-HBEC in the plasma was determined in 170 patients with COPD and 150 age-matched healthy controls...
2016: Clinical Laboratory
Limin Sun, Ximing Qi, Qiang Tan, Hongmei Yang, Xiangqian Qi
BACKGROUND: Recently, butyrylcholinesterase (BChE) activity seems to have an independent prognostic role in acute coronary syndrome (ACS). However, the underlying mechanisms remain unclear. A previous study showed that serum BChE activity had a diagnostic value for chronic heart failure. This raises a question: whether BChE activity is associated with cardiac function in ACS, and if so, is this association related to the predictive value of BChE? The aim of this study was to determine the association between BChE activity with cardiac function assessed by Killip class and left ventricular ejection fraction (LVEF) in acute myocardial infarction (AMI) and to evaluate the independent prognostic role of BChE with consideration of these two indicators...
2016: Clinical Laboratory
Ruanmin Zou, Wangkai Xie, Huijing Wang, Jian Wang, Lanlan Xiao, Shuhui Ji, Xiangmin Chen, Lifang Zhang, Xiangyang Xue, Jun Chen
BACKGROUND: Persistent high-risk HPV infection is a major cause of cervical cancer and E6/E7 genes and the Li gene in the HPV genome are key targets to detect high-risk HPV. This study aims to explore the relationship between cervical lesions and E6/7 by establishing a polymerase chain reaction (PCR) to detect multiplex genes based on HPV EE7 genes. It is hoped that such methods will provide a more reliable method for clinical screening and the prevention of cervical cancer. METHODS: Based on alignment, specific primers were designed for HPV E6/E7 genes, the sequences of which came from five5 high-risk papillomaviruses that are common in China...
2016: Clinical Laboratory
Monika Gudowska, Ewa Gruszewska, Bogdan Cylwik, Anatol Panasiuk, Robert Filisiak, Maciej Szmitkowski, Lech Chrostek
BACKGROUND: The great significance for the metabolism of lipoproteins is the composition of carbohydrate chain of apolipoproteins, where sialic acid (SA) is located. In VILDL and LDL sialic acid is attached to apolipoprotein B. The sialylation of serum proteins including apolipoprotein B can be affected in the course of liver diseases. Therefore, the aim of this study was to assess the effect of liver diseases on the concentration and content of SA in ApoB-containing lipoproteins. METHODS: The tested group consisted of 165 patients (118 males, 47 females) with liver diseases: alcoholic cirrhosis, non-alcoholic cirrhosis, chronic hepatitis, toxic hepatitis, chronic viral hepatitis, and liver cancer...
2016: Clinical Laboratory
Christoph Edlinger, Bernhard Wernly, Michael Leisch, Jürgen Kammler, Alexander Kypta, Sarah Eder, Christian Jung, Uta C Hoppe, Michael Lichtenauer
BACKGROUND: Contradictory results of large clinical trials of stem cell therapy in acute myocardial infarction (AMI) have impeded a wider clinical use. As signalling via paracrine factors in AMI has received more and more attention recently, we sought to compare processing protocols with special emphasis on interleukin-6 (IL-6), a factor of major relevance for triggering cardioprotective signals via STAT3 and PI3K. METHODS: Bone marrow and peripheral blood mononuclear cells were processed according to protocols used in the REPAIR-AMI and ASTAMI study...
2016: Clinical Laboratory
Shu Feng, Bin Wei, Chenli Rao, Tingting Wang, Yuling Xiao, Chuanmin Tao, Lanlan Wang
BACKGROUND: Detection of serum hepatitis B virus (HBV) markers is important for rapid screening and diagnosis of HBV infection. In this study, the newly launched HISCL-5000 analyzer (Sysmex Corporation, Kobe, Japan) was appraised in parallel with the MODULAR E170 (Roche Diagnostics, Penzberg, Germany), with regard to the detection of serological HBV markers in China for comparing and evaluating their results and capability. METHODS: In this study, a total of 5,662 clinical serum samples were tested with the two automated systems...
2016: Clinical Laboratory
Ichiro Wakabayashi
BACKGROUND: Cardiometabolic index (CMI) is a new index for discriminating diabetes mellitus and has been demonstrated to be associated with the degree of atherosclerosis in patients with peripheral arterial disease. The purpose of this study was to clarify the relationship of CMI with smoking, a major risk factor for atherosclerotic disease. METHODS: The subjects included 31,742 Japanese men (35 - 60 years old) receiving health-checkup examinations at their workplaces...
2016: Clinical Laboratory
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