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Clinical Laboratory

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https://www.readbyqxmd.com/read/29035465/enhanced-specificity-of-braf-v600e-genotyping-using-wild-type-blocker-coupled-with-internal-competitive-reference-in-a-single-tube
#1
Jia Peng, Kun Wei, Shu Yu, Xiang Yang, Xiang Zhao, Yu Liu, Xiao-Yan Zhu, Na Zhao, Qing Huang, Wei-Ling Fu
BACKGROUND: Mutations in the BRAF gene have been strongly associated with failure in cancer treatment using epidermal growth factor receptor (EGFR) antibodies. To better diagnose and assess the prognosis of cancer patients, mutation screening of the BRAFV600E gene should be performed prior to clinical anti-tumor drug therapy to avoid ineffective treatment. METHODS: In our previous study, we developed a real-time wild-type blocking PCR (WTB-PCR), which can amplify the mutant allele at high efficiency while simultaneously inhibiting the amplification of wild-type alleles...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29035464/galectin-3-reflects-left-atrial-function-being-assessed-by-cardiac-magnetic-resonance-imaging
#2
Seung-Hyun Kim, Michael Behnes, Michele Natale, Sonja Hamed, Julia Hoffmann, Ursula Hoffmann, Johannes Budjan, Siegfried Lang, Martin Borggrefe, Theano Papavassiliu, Thomas Bertsch, Ibrahim Akin
BACKGROUND: This study aims to evaluate the association between galectin-3 and left atrial function (LAF) in patients undergoing cMRI. METHODS: Patients undergoing cMRI were prospectively enrolled. Right ventricular dysfunction (< 50%) was excluded. Blood samples for biomarker measurements of galectin-3 and NT-proBNP were collected at the time of cMRI examination. RESULTS: A total of 84 patients were included. Median LVEF was 59% (IQR 51 - 64%)...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29035463/effect-of-balanced-crystalloid-gelatin-and-hydroxyethyl-starch-on-coagulation-detected-by-rotational-thromboelastometry-in-vitro
#3
Silvie Sevcikova, Tomas Vymazal, Miroslav Durila
BACKGROUND: Fluid resuscitation with crystalloid and colloid solutions is a common treatment in perioperative medicine. However, a variety of unbalanced or balanced solutions are used in clinical practice and there is still vivid debate going on regarding selection of optimal fluid with minimal negative effect on coagulation to minimize bleeding and blood transfusion requirements. The aim of the study was to investigate adverse effects of balanced crystalloids and colloids on coagulation measured by thromboelastometry in vitro...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29035462/cpg-odn-stimulation-in-population-screening-monoclonal-b-cell-lymphocytosis
#4
Helena Podgornik, Petra Bajuk, Tadej Furlan, Hermina Cibej, Ana D Kebe, Peter Cernelc
BACKGROUND: Synthetic CpG-oligodeoxynucleotides (CpG-ODN) induce proliferation in normal and malignant lymphocytes B (LyB). This effect is widely exploited in CLL conventional chromosome banding analysis (CBA), which has become reliable only after the cultivation of CLL LyB using CpG-ODN stimulation in combination with IL-2. Monoclonal B-cell lymphocytosis (MBL) differs from CLL mainly in clone size. Only cytogenetic data on recurrent chromosomal aberrations analyzed by fluorescence in situ hybridization (FISH) are available for population screening MBL (sMBL)...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29035461/evaluating-efemp1-in-cerebrospinal-fluid-and-serum-as-a-potential-diagnosis-biomarker-for-meningiomas
#5
Li Jia, Chen Liu, Ying Xin, Aimin Zhang, Yunli Zhou, Dong Dong, Li Ren
BACKGROUND: The aim of this study was to determine the cerebrospinal fluid (CSF) and serum levels of EGF containing fibulin-like extracellular matrix protein 1 (EFEMP1) in the patients with meningiomas and explore its potential as a biomarker. METHODS: Forty-five patients with meningioma, 11 of whom underwent meningioma resection, as well as 30 healthy controls were enrolled in this study. CSF and blood samples were collected preoperatively and postoperatively. Expression levels of EFEMP1 were measured by enzyme-linked immunosorbent assay (ELISA)...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29035460/pleural-alveolar-rhabdomyosarcoma
#6
Hongyang Lu, Jing Qin, Zeng Wang
Alveolar rhabdomyosarcoma (ARMS) commonly occurring in children and adolescents, is a rare and aggressive soft tissue malignancy. Here, a rare case of pleural ARMS is reported in a boy presenting dyspnea for two weeks. The pathology of a biopsy specimen from pleura showed ARMS. PAX3-FKHR fusion protein is positive and the PAX7-FKHR fusion protein is negative as detected by fluorescence in situ hybridization. The prognosis of this patient was poor and nonresponsive to chemotherapy.
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29035459/level-of-platelet-distribution-width-and-outcome-prediction-in-patients-with-traumatic-brain-injury
#7
Biao Zhang, Jianjun Gu, Yizhen Qiu, Jin Zhao, Yi Song, Huojun Jiang, Aliang Ding, Xiaoqi Chen, Rongming Yu, Guofang Liu, Aihao Hu
BACKGROUND: This study examines the clinical utility of the level of increased platelet distribution width (PDW) as a predictor of outcome in patients with traumatic brain injury. METHODS: In this retrospective study, 120 patients with traumatic brain injury (TBI) were recruited. Age, gender, PDW, and Glasgow Coma Scale (GCS) scores were measured. These patients were divided into two groups based on their 30-day outcomes. Receiver operating curves (ROCs) were generated to identify predictors of 30-day mortality...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29035458/concordance-of-mutation-detection-in-circulating-tumor-dna-in-early-clinical-trials-using-different-blood-collection-protocols
#8
Lise B Ahlborn, Mette Madsen, Lars Jonson, Finn C Nielsen, Ulrik Lassen, Christina W Yde, Morten Mau-Sorensen
BACKGROUND: Small fragments of tumor DNA can be found in the circulation of cancer patients, providing a noninvasive access to tumor material (liquid biopsy). Analysis of circulating tumor DNA (ctDNA) has been used for diagnosis, treatment decisions, and detection of therapy resistance, including in patients with tumors inaccessible for biopsy, making ctDNA an important alternative source of tumor material. Immediate separation of plasma is widely used in standard isolation of cell-free DNA to ensure high quality plasma DNA...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29035457/evaluation-of-mir-203-expression-levels-and-dna-promoter-methylation-status-in-serum-of-patients-with-endometrial-cancer
#9
Marco Benati, Martina Montagnana, Elisa Danese, Elisa Paviati, Silvia Giudici, Massimo Franchi, Giuseppe Lippi
BACKGROUND: Endometrial cancer (EC) is currently considered the fourth most frequent female cancer in Europe. In an attempt to achieve an early diagnosis, many studies have identified some putative biomarkers for gynecologic cancers, including circulating microRNAs (miRs) and aberrant promoter methylation status. Previous studies which have investigated miR-203 expression profiles in EC tissues and normal endometrial tissues concluded that miR-203 is regulated by methylation promoter...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29035456/circulating-mir-29b-and-mir-424-as-prognostic-markers-in-patients-with-acute-cerebral-infarction
#10
Yue-Zhan Zhang, Jun Wang, Feng Xu
BACKGROUND: Down-regulation of miR-29b and miR-424 have been observed in patients with acute cerebral infarction (ACI); however, the clinical significance of circulating miR-29b and miR-424 as prognostic markers still requires further investigation. METHODS: A total of 45 patients diagnosed with ACI and 45 healthy volunteers were enrolled in this study, and the clinical information of the patients were collected. The serum samples of the participants were collected and stored at -80°C...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29035455/evaluation-of-a-magnetic-cellulose-based-dna-extraction-system-to-improve-the-performance-of-hybribio-human-papillomavirus-genotyping-and-screening-tests-for-cervical-swab-samples
#11
Zengge Wang, Yulin Zhou, Jun Ren, Zhongmin Xia, Qiwei Guo
BACKGROUND: To ensure the accuracy of clinical human papillomavirus (HPV) testing, the nucleic acid extraction procedure should be thoroughly evaluated for each clinical sample type. Therefore, we evaluated whether the MagCore® Automated Nucleic Acid Extraction system (MagCore system) could improve HybriBio HPV test performance for cervical swab samples. METHODS: We compared the performance of HybriBio HPV genotyping and screening tests using samples prepared with the MagCore system and Cell Lysis Kit, which was provided by the HPV test manufacturer...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29035454/the-relationship-between-mdm2-t309g-polymorphism-and-leukemia-in-the-chinese-population-evidence-from-a-meta-analysis
#12
Dewan Zhao, Ting Liu
BACKGROUND: Increasing numbers of studies have been carried out on the association of MDM2 T309G polymorphism with susceptibility to leukemia and have generated conflicting results. This meta-analysis updated and revaluated the possible associations between MDM2 T309G polymorphism and leukemia in the Chinese population. METHODS: The PubMed, Springer Link, Ovid, Chinese Wanfang Data Knowledge Service Platform, Chinese National Knowledge Infrastructure, and Chinese Biology Medicine were searched up to February 2017...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29035453/immunophenotyping-of-chronic-lymphocytic-leukemia
#13
Mesude Falay And Gulsum Ozet
BACKGROUND: Chronic Lymphocytic Leukemia (CLL) is one of the most common diagnoses made by flow cytometry laboratories. There is no consensus on which markers need to be used in flow cytometry for accurate immunophenotyping. Herein, we investigated the role of markers used in flow cytometry in the distinction between CLL and MCL. METHODS: A total 339 recently diagnosed B lymphoproliferative patient cases were retrospectively studied for their immunophenotypical propoerties using flow cytometry...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29035452/role-of-cytokines-in-the-pathogenesis-of-visceral-leishmaniasis
#14
REVIEW
Ramesh Kumar, Madhav Bhatia, Kalpana Pai
Infection in humans with Leishmania manifests into a spectrum of diseases. The manifestations of the disease depend on the resultant evasion of the parasite to immune responses namely macrophages, which is an exclusive host of leishmania. The B cells valiantly mount antibody responses, however to no avail as the Leishmania parasites occupy the intracellular niches of the macrophages. Extensive studies have been documented on the role of cell-mediated immunity (CMI) in protection and counter survival strategies of the parasites leading to down-regulation of CMI...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29035451/investigations-of-the-serum-free-light-chain-ratio-according-to-the-updated-2014-imwg-diagnostic-criteria-for-multiple-myeloma-in-korean-patients-with-newly-diagnosed-multiple-myeloma
#15
LETTER
Hyung-Seok Yang, Yujin Han, Young Jin Kim, Anbok Lee, Sun Young Cho
No abstract text is available yet for this article.
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29035450/imbalance-between-matrix-metalloproteinases-and-tissue-inhibitors-of-metalloproteinases-promotes-invasion-and-metastasis-of-head-and-neck-squamous-cell-carcinoma
#16
Adina E Stanciu, Adina Zamfir-Chiru-Anton, Marcel M Stanciu, Cristian R Popescu, Dan C Gheorghe
BACKGROUND: The balance between collagenolysis and its inhibition is critical during extracellular matrix remodeling in head and neck squamous cell carcinoma (HNSCC). An imbalance between matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) plays a critical role in the development of tumor cell invasion and metastasis. The purpose of the study was to determine MMP-9/TIMP-1 and MMP-2/TIMP-2 ratios in the sera of patients with HNSCC in relation to clinicopathological parameters...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29035449/performance-verification-of-the-iris-iq200-sprint-automated-urine-microscopy-analyzer-in-a-hospital-routine-laboratory
#17
Ming Cui, Shaoqing Ju, Yingjuan Shi, Rongrong Jing
BACKGROUND: Systematic performance verification is required before a laboratory can introduce a new measurement procedure for reporting results of patient testing. The aim of this study was to determine whether a new Iris iQ200 Sprint automated urine microscopy analyzer (iQ200 Sprint) could be incorporated into our routine laboratory. METHODS: A total of 421 fresh urine samples were selected from the Affiliated Hospital of Nantong University, including those from healthy individuals and those with a variety of abnormalities to ensure a wide range of results...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29035448/a-case-study-in-advanced-lung-cancer-patients-with-vimentin-over-expression
#18
Nagla A Karim, Ihab Eldessouki, Mahendar Yellu, Tariq Namad, Jiang Wang, Ola Gaber
BACKGROUND: Vimentin belongs to an intermediate filament (IF) family of proteins, mainly present in mesenchymal cells and has a crucial role in maintaining cellular integrity. Vimentin can induce epithelial to mesenchymal transition, and thus increase migration and invasion capacity of the cells. It has been shown to be a useful and reliable diagnostic and prognostic marker in several cancers including colon cancers, breast and hepatocellular cancers. Recent studies suggest that it may have a role in distant metastasis of non-small cell lung cancer (NSCLC) accounting for poor survival [1]...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29035447/porphyromonas-gingivalis-infection-accelerates-atherosclerosis-mediated-by-oxidative-stress-and-inflammatory-responses-in-apoe-mice
#19
Yan Xuan, Qiao Shi, Guo-Jing Liu, Qing-Xian Luan, Yu Cai
BACKGROUND: The periodontal pathogen Porphyromonas gingivalis (P. gingivalis) has been proven to accelerate the development of atherosclerosis in apolipoprotein E (ApoE)-deficient mice. In this study, we used an ApoE knockout (ApoE-/-) mouse model with chronic intravenous infection with P. gingivalis to investigate the possible mechanisms of P. gingivalis-induced atherosclerosis. METHODS: Eight-week-old ApoE-/- mice were randomly assigned to two groups: (a) ApoE-/- + PBS (n = 8); (b) ApoE-/- + P...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29035446/the-effects-of-insulin-and-glucose-on-different-characteristics-of-a-upec-alterations-in-growth-rate-and-expression-levels-of-some-virulence-genes
#20
Defne Gumus, Emre Yoruk, Fatma Kalayci-Yuksek, Gulsen Uz, Aysegul Topal-Sarikaya, Mine Ang-Kucuker
BACKGROUND: Host factors are known to modulate virulence, antibiotic susceptibility, and growth rate of bacteria. The effect of human insulin and glucose on growth rate and expression of virulence genes (usp, sfa/foc, cnf1) of a uropathogenic E. coli (UPEC) strain were investigated in this study. METHODS: E. coli C7 was grown in tryptic soy broth (TSB-control) and TSB containing 20 µU/mL insulin, 200 µU/mL insulin, 0.1% glucose, and 200 µU/mL insulin + 0.1% glucose...
October 1, 2017: Clinical Laboratory
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