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Clinical Laboratory

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https://www.readbyqxmd.com/read/28182364/a-rare-case-of-etv6-mecom-rearrangement-in-therapy-related-acute-myeloid-leukemia-with-t-3-12-and-monosomy-7
#1
LETTER
Young Jin Kim, John J Yang, Yujin Han, Suekyeung Kim, Hyung-Seok Yang, Hwi-Joong Yoon, Ju Hie Lee, Woo-In Lee, Tae Sung Park
No abstract text is available yet for this article.
February 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28182363/comprehensive-analysis-of-lncrna-and-mrna-expression-profiles-in-lung-cancer
#2
Ran Chen, Wen-Xing Li, Yan Sun, Yong Duan, Qing Li, Ai-Xing Zhang, Jia-Lun Hu, Yu-Ming Wang, Yue-Dong Gao
BACKGROUND: Lung cancer is one of the most common malignancies across the world. Long noncoding RNAs (lncRNAs) play an important role in the pathology. This study was to compare the lncRNA and mRNA of lung cancer. The aim of this study was to compare the lncRNA and mRNA expression profiles, their related biological functions, and pathways in lung cancer. METHODS: Human lung cancer mRNA expression datasets were searched and downloaded from NCBI-GEO. LncRNA expression profiles in lung cancer were detected by the Agilent Human LncRNA Microarray V3...
February 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28182362/upregulation-of-foxo-1-signaling-mediates-the-proinflammatory-cytokine-upregulation-in-the-macrophage-from-polycystic-ovary-syndrome-patients
#3
Ning Li, Xiaoyan Wang, Xiaojie Wang, Hongna Yu, Li Lin, Chengming Sun, Peng Liu, Yongli Chu, Jianqing Hou
BACKGROUND: Chronic activation of macrophage-mediated inflammatory signals in insulin-sensitive metabolic tissues is thought to be one of the causes of insulin resistance-one of the hallmarks of the metabolic syndrome. Insulin resistance is a feature of polycystic ovary syndrome (PCOS) and is related to mitochondrial and endothelial function. METHODS: In the present study, we investigated the phosphorylation level of FoxO 1, which is suppressed by the action of AKT, triggers the TLR4 inflammatory signaling pathway in the macrophages, from polycystic ovary syndrome patients or normal subjects...
February 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28182361/evaluation-of-line-immunoassay-to-detect-htlv-1-infection-in-an-endemic-area-southwestern-japan-comparison-with-polymerase-chain-reaction-and-western-blot
#4
Kazumi Umeki, Kunihiko Umekita, Yuuki Hashikura, Ikuo Yamamoto, Kazuyoshi Kubo, Yasuhiro Nagatomo, Akihiko Okayama
BACKGROUND: Human T-lymphotropic virus type 1 (HTLV-1) has been recognized as a cause of adult T-cell leukemia/lymphoma, HTLV-1-associated myelopathy/tropical spastic paraparesis, and HTLV-1-associated uveitis. HTLV-1 infection is normally detected by screening for HTLV-1 antibodies, and positive samples are confirmed by Western blot (WB). However, WB fails to confirm some samples that were positive for HTLV-1 antibodies on screening. Line immunoassay (LIA) is commonly used in Europe and Brazil, but not in Japan...
February 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28182360/mutation-analysis-in-classical-phenylketonuria-patients-followed-by-detecting-haplotypes-linked-to-some-pah-mutations
#5
Fatemeh Dehghanian, Mohammad Silawi, Seyed M B Tabei
BACKGROUND: Deficiency of phenylalanine hydroxylase (PAH) enzyme and elevation of phenylalanine in body fluids cause phenylketonuria (PKU). The gold standard for confirming PKU and PAH deficiency is detecting causal mutations by direct sequencing of the coding exons and splicing involved sequences of the PAH gene. Furthermore, haplotype analysis could be considered as an auxiliary approach for detecting PKU causative mutations before direct sequencing of the PAH gene by making comparisons between prior detected mutation linked-haplotypes and new PKU case haplotypes with undetermined mutations...
February 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28182359/fluorescence-in-situ-hybridization-with-the-urovysion-kit-for-the-detection-of-biliary-cancer-in-chinese-patients
#6
Xi Wu, Xuan Zeng, Aiming Yang, Fang Yao, Tao Guo, Dongsheng Wu, Shafei Wu, Hongmei Qiu, Weixun Zhou, Zhen Huo, Xinghua Lu, Jiaming Qian
BACKGROUND: Conventional biliary brush via ERCP has low clinical detection for biliary malignancy. Therefore, new approaches are needed to facilitate diagnosis. We therefore explored the application of fluorescent in situ hybrization (FISH) using a UroVysion kit for the detection of malignancy in the bile duct. METHODS: Genetic alterations of target chromosomes such as aneuploidy in Chinese biliary cancer cell lines and tissues were measured using a UroVysion kit...
February 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28182358/aetiology-and-significance-of-hospital-acquired-infections-in-mexico
#7
REVIEW
Nayelli M Alonso-Aguilar, Sara R Juarez-Enriquez, Graciela Castro-Escarpulli, Gildardo Rivera, Virgilio Bocanegra-Garcia, Xianwu Guo, Julieta Luna-Herrera, M G Aguilera-Arreola
Hospital-acquired infections (HAIs) are infections that develop in the hospital environment and can be acquired by a patient or hospital staff. They are complications that combine diverse risk factors that make an individual susceptible and are frequently caused by endogenous and exogenous bacterial agents. The most commonly studied etiological agents are bacteria and fungi, with the former representing the most common etiological agents reported to the Hospital Epidemiological Surveillance Network (RHOVE) between 2007 and 2012...
February 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28182357/influence-of-in-vitro-hemolysis-on-80-different-laboratory-tests
#8
Yasuhiro Yanagisawa, Kazumasa Isobe, Asami Naito, Michikuni Ishijima, Toru Nanmoku, Takayuki Yamamoto, Etsu Suzuki, Yasushi Kawakami
BACKGROUND: In vitro hemolysis is probably the most common pre-analytic problem in laboratory medicine. However, it introduces variation into results in unknown ways. Therefore, the purpose of this study was to assess the quantitative effects of hemolysis on 80 different, routine laboratory tests. METHODS: We examined the ratio of hemolysis in our hospital from January 1 to March 31, 2015. Next, to study the effect of in vitro hemolysis of whole blood, we added lysed erythrocytes to pooled specimens of serum or plasma to give hemoglobin concentrations of 0...
February 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28182356/association-between-n-acetyltransferase-2-polymorphism-and-bladder-cancer-risk-a-meta-analysis-in-a-single-ethnic-group
#9
Wan-Jiang Xu, Li-Ping Wen, Xiang-Xin Jiang, Li-Yin Ye, Fan-Hua Meng, Sheng Guan, Ying-Jun Qian, Jing-Feng Wei
BACKGROUND: Many studies have evaluated the correlation between N-acetyltransferase 2 (NAT2) slow acetylation genotype and bladder cancer risk. However, the results are inconsistent and remain to be confirmed in each ethnic group. To assess the effects of NAT2 acetylation status on the risk of bladder cancer in the Chinese population, a meta-analysis was performed. METHODS: Studies were identified using PubMed and Chinese databases through February 2016. The associations were assessed with pooled odds ratios (ORs) and 95% confidence intervals (CIs)...
February 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28182355/pure-red-cell-aplasia-associated-with-monoclonal-gammopathy-of-undetermined-significance-and-literature-review
#10
Hyunjung Gu, Woo-In Lee, You La Jeon, So Young Kang, Myeong Hee Kim, Tae Sung Park
BACKGROUND: Pure red cell aplasia (PRCA) is an uncommon disease which involves an almost complete absence of the erythroid lineage in bone marrow (BM) and causes severe anemia. Cases due to monoclonal gammopathy occurring in plasma cell disorder have been infrequently reported. Here we report a case of PRCA associated plasma cell disorder, especially monoclonal gammopathy of undetermined significance (MGUS). METHODS: A 55-year-old male visited the ER due to general weakness...
February 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28182354/exploring-the-molecular-mechanism-and-biomarker-of-recurrent-aphthous-stomatitis-based-on-gene-expression-microarray
#11
Shang Dan, Zhang Jinwei, Zhang Qiang, Shang Jianwei, Zhang Weijun
BACKGROUND: Recurrent aphthous stomatitis (RAS) is one of the most common chronic oral diseases with morbidity ranging from 5% to 20%. The pathogenesis is still not fully understood though immune dysregulation and local trauma have been implicated and the curative effect that can be achieved through current therapy is mainly to alleviate the pain. So, the understanding of its molecular mechanisms can allow the prevention and treatment of RAS at the molecular level. METHODS: 14 normal tissues and 14 ulcerated tissues were subjected to Affymetrix Human Genome U133 Plus 2...
February 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28182353/prognostic-value-of-ki67-expression-for-patients-with-surgically-resected-hepatocellular-carcinoma-perspectives-from-a-high-incidence-area
#12
Kai Bai, Yi Cao, Qiaojia Huang, Yi Jiang, Lizhi Lv
BACKGROUND: Antigen Ki-67 (Ki67) is a documented tumor biomarker known to be aberrantly expressed in many human cancers. However, to our knowledge, its prognostic value for surgically resected hepatocellular carcinoma (HCC) for high incidence areas of China has not been described. METHODS: We retrospectively investigated 339 patients with HCC who underwent surgery to remove the primary cancer at one center in Fujian, China, an area with a high incidence of HCC from 2004 to 2012...
February 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28182352/changed-profile-of-serum-transferrin-isoforms-in-liver-diseases
#13
Monika Gudowska, Ewa Gruszewska, Anatol Panasiuk, Bogdan Cylwik, Magdalena Swiderska, Robert Filisiak, Maciej Szmitkowski, Lech Chrostek
BACKGROUND: The aim of the study was to assess the effect of liver diseases on the serum profile of transferrin isoforms. METHODS: Patients with alcoholic cirrhosis (AC) - 63 subjects, non-alcoholic cirrhosis (NAC) - 28, and toxic hepatitis (HT) - 32 were studied. The cirrhotic patients were classified according to the Child-Pugh scale. Samples were analyzed by capillary electrophoresis with the MINICAP system. RESULTS: Significant differences were noted in the relative concentrations of disialotransferrin in HT patients (mean ± SD; 1...
February 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28182351/increased-red-cell-distribution-width-is-strong-inflammatory-marker-of-liver-diseases-in-a-guangxi-population
#14
Fang Lan, Huiping Wei, Xuan Zhu, Shan Li, Xue Qin
BACKGROUND: Red cell distribution width (RDW), part of a routine complete blood count in a clinical laboratory, has been widely and routinely used in the diagnosis of various diseases. The aim of the present study was to investigate the relationship between increased RDW and liver diseases and whether RDW is a new inflammatory marker for liver diseases in a Guangxi population. METHODS: A total of 735 patients were enrolled in our study, including 113 patients with chronic hepatitis B (CHB), 133 liver cirrhosis (LC) patients, 105 patients with hepatocellular carcinoma (HCC), 55 alcoholic hepatitis (AH) patients, 44 chronic hepatitis C (CHC) patients, and 285 healthy persons...
February 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28182350/diagnostic-value-of-serum-hsa-mir-92a-in-patients-with-cervical-cancer
#15
Qianqian Kong, Zhenhua Tang, Fenfen Xiang, Jiemin Jiang, Honghong Yue, Rong Wu, Xiangdong Kang
BACKGROUND: Hsa-mir-92a acts as an onco-miRNA and may contribute to the progression and invasion of cervical cancer, which is a common malignant tumor in women worldwide. The objective of the present study was to evaluate whether serum hsa-mir-92a could serve as a diagnostic biomarker for cervical cancer patients. METHODS: The expression levels of hsa-mir-92a were analyzed in the serum of patients with CIN I, CIN II, CIN III, cervical cancer Ia - IIa and compared with those of the control group samples...
February 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28182349/the-a445c-variant-in-apelin-receptor-and-diabetic-retinopathy-in-tunisian-patients
#16
Hayet Soualmia, Fatma Midani, Sondess Hadj-Fradj, Nedra Saadi, Mehdi Lassoued, Taieb Messoud, Monia Gharbi, Zohra Ben-Amor
BACKGROUND: Apelin and apelin receptor (APLN/APLNR) are involved in the retinal neovascularization of diabetic retinopathy (DR). METHODS: This study investigated the impact of the APLNR A445C variant on the risk of DR in a sample of the Tunisian population (100 patients with DR and 105 healthy controls) using PCR-RFLP. RESULTS: The genotype frequencies of the APLNR A445C variant were not significantly different between the patient and control groups...
February 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28182348/a-comparison-of-four-methods-immunochemistry-and-hplc-for-determination-of-25-oh-vitamin-d-in-postmenopausal-women
#17
Eva Klapkova, Jana Cepova, Marta Pechova, Katerina Dunovska, Karel Kotaska, Richard Prusa
BACKGROUND: Three immunochemical methods for the determination of 25-(OH)-vitamin D and validated HPLC method for the determination of 25-(OH)-vitamin D3 and 25-(OH)-vitamin D2 were compared. 62 patient samples from postmenopausal women were measured and the results obtained by all these methods were compared. METHODS: We used three chemiluminescent assays for determination of 25-(OH)-vitamin D. 25-(OH)-vitamin D3 and 25-(OH)-vitamin D2 were determined by HPLC with UV detection (Agilent 1200)...
February 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28182347/the-diagnostic-value-of-procalcitonin-versus-other-biomarkers-in-prediction-of-bloodstream-infection
#18
Ya-Ping Pan, Ya-Ping Fang, Yuan-Hong Xu, Zhong-Xin Wang, Ji-Lu Shen
BACKGROUND: We compared the diagnostic utility of procalcitonin (PCT), C-reactive protein (CRP), and hematological markers, including white blood cell count (WBC), neutrophils (NEU), percentage of neutrophils (NEU%), lymphocytes (LYM), neutrophil-lymphocyte count ratio (NLCR), and platelet count (PLT) for predicting bloodstream infection (BSI), which was confirmed by blood culture (BC). METHODS: A retrospective analysis was conducted for 1807 inpatients. The level of PCT, CRP, blood cells, and blood culture results were compared between the positive blood culture group and negative blood culture group; each indicator was analyzed in the performance of bacterial BSI diagnosis by drawing ROC curves...
February 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28182346/correction-equation-for-estimation-of-actual-potassium-concentration-in-hemolyzed-specimen
#19
Tae Sic Lee, Juwon Kim, Young Uh, Yon Chul Park, Gil Sung Yoo, Kap Jun Yoon
BACKGROUND: A spuriously elevated serum potassium value could possibly cause medical decision errors because it leads to masked hypokalemia or pseudohyperkalemia. The aim of this study was to develop a correction equation for falsely elevated potassium level caused by hemolysis. METHODS: A total of 988 samples with a hemolysis index (HI) value greater than the potassium alert HI value were recollected within two hours from initial collection. We divided 988 paired samples into 3 groups: hypokalemia, normal, and hyperkalemia...
February 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28182345/a-cross-validation-design-using-age-and-hla-alleles-for-the-assessment-of-clinical-schizophrenia-risk
#20
Song Laiyu, Zhang Lei, Xie Soufu, Xv Junting, Zhou Jie, Liu Hui
BACKGROUND: Schizophrenia (SCH) is a highly heritable disease that occurs mostly in young adults. Genetic factors usually play important roles in the onset of SCH. Human leukocyte antigen complex (HLA) genes are considered to be the important genetic predisposition factor and the genetic markers in SCH and other diseases. METHODS: To screen SCH-associated HLA alleles, alleles for HLA-DRB1 and HLA-DQB1 loci were determined using the PCR-SSP typing kit. Statistically significant differences between observed and expected frequencies of allele combinations were identified...
February 1, 2017: Clinical Laboratory
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