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Journal of Industrial Microbiology & Biotechnology

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https://www.readbyqxmd.com/read/28509952/high-yield-production-of-mannitol-by-leuconostoc-pseudomesenteroides-ctcc-g123-from-chicory-derived-inulin-hydrolysate
#1
Min Zhang, Lei Gu, Chao Cheng, Junru Zhu, Hao Wu, Jiangfeng Ma, Weiliang Dong, Xiangping Kong, Min Jiang, Pingkai Ouyang
Chicory is an agricultural plant with considerable potential as a carbohydrate substrate for low-cost production of biochemicals. In this work, the production of mannitol by Leuconostoc pseudomesenteroides CTCC G123 from chicory-derived inulin hydrolysate was investigated. The bioconversion process initially suffered from the leakage of fructose to the phosphoketolase pathway, resulting in a low mannitol yield. When inulin hydrolysate was supplemented with glucose as a substrate for mannitol production in combination with aeration induction and nicotinic acid induced redox modulation strategies, the mannitol yield greatly improved...
May 16, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28508118/effects-of-aeration-on-metabolic-profiles-of-mortierella-alpina-during-the-production-of-arachidonic-acid
#2
Xin Zhang, Ling Jiang, Li-Ying Zhu, Qin-Ke Shen, Xiao-Jun Ji, He Huang, Hong-Man Zhang
To investigate the metabolic regulation against oxygen supply, comparative metabolomics was performed to explore the metabolic responses of Mortierella alpina in the process of arachidonic acid (ARA) production. More than 110 metabolites involved in Embden-Meyerhof-Parnas pathway, pentose phosphate pathway, tricarboxylic acid cycle, inositol phosphate metabolism, fatty acid biosynthesis, and amino acid metabolism were identified by gas chromatography-mass spectrometry. Samples at different aeration rates were clearly distinguished by principal components analysis and partial least squares analysis, indicating that oxygen supply had a profound effect on the metabolism of M...
May 15, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28497282/highly-complex-substrates-lead-to-dynamic-bacterial-community-for-polyhydroxyalkanoates-production
#3
Diogo Queirós, Alexandre Fonseca, Simona Rossetti, Luísa S Serafim, Paulo C Lemos
Mixed microbial cultures (MMC) and waste/surplus substrates, as hardwood spent sulfite liquor, are being used to decrease polyhydroxyalkanoates' (PHA) production costs. The process involves two or three steps, being the selection step a crucial one. For the industrial implementation of this strategy, reactor stability in terms of both performance and microbial community presence has to be considered. A long-term operation of a sequencing batch reactor under feast/famine conditions was performed along with microbial community identification/quantification using FISH and DGGE...
May 11, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28451838/improved-cider-fermentation-performance-and-quality-with-newly-generated-saccharomyces-cerevisiae%C3%A2-%C3%A3-%C3%A2-saccharomyces-eubayanus-hybrids
#4
Frederico Magalhães, Kristoffer Krogerus, Virve Vidgren, Mari Sandell, Brian Gibson
Yeast cryotolerance may be advantageous for cider making, where low temperatures are usually employed. Here, we crossed the cryotolerant S. eubayanus with a S. cerevisiae wine strain and assessed the suitability of the hybrids for low-temperature cider fermentation. All strains fermented the juice to 5% ABV, but at different rates; hybrid strains outperformed S. cerevisiae, which was sensitive to low temperatures. The best hybrid fermented similarly to S. eubayanus. S. eubayanus produced sulphurous off flavours which masked a high concentration of fruity ester notes...
April 27, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28451837/high-level-productivity-of-%C3%AE-%C3%AF-dodecanedioic-acid-with-a-newly-isolated-candida-viswanathii-strain
#5
Weifeng Cao, Hongbao Li, Jianquan Luo, Junxiang Yin, Yinhua Wan
α,ω-Dicarboxylic acids (DC) are versatile chemical intermediates with different chain lengths, which are well-known as polymer building block. In this work, a new strain with high productivity of DC was isolated from oil-contaminated soil. Based on the morphology and phylogenetic analyses of the internal transcribed spacer sequences, it was characterized as Candida viswanathii. It was found that the contribution of carbon flux to the cell growth and DC production from n-dodecane could be regulated by the sucrose and yeast extract concentrations in the medium, and besides the broth pH, a suitable proportioning of sucrose and yeast extract was the key to achieve the optimal transition from cell growth phase to DC production phase...
April 27, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28444480/model-based-biotechnological-potential-analysis-of-kluyveromyces-marxianus-central-metabolism
#6
A Pentjuss, E Stalidzans, J Liepins, A Kokina, J Martynova, P Zikmanis, I Mozga, R Scherbaka, H Hartman, M G Poolman, D A Fell, A Vigants
The non-conventional yeast Kluyveromyces marxianus is an emerging industrial producer for many biotechnological processes. Here, we show the application of a biomass-linked stoichiometric model of central metabolism that is experimentally validated, and mass and charge balanced for assessing the carbon conversion efficiency of wild type and modified K. marxianus. Pairs of substrates (lactose, glucose, inulin, xylose) and products (ethanol, acetate, lactate, glycerol, ethyl acetate, succinate, glutamate, phenylethanol and phenylalanine) are examined by various modelling and optimisation methods...
April 25, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28439768/discrimination-of-wine-lactic-acid-bacteria-by-raman-spectroscopy
#7
Susan B Rodriguez, Mark A Thornton, Roy J Thornton
Species of Lactobacillus, Pediococcus, Oenococcus, and Leuconostoc play an important role in winemaking, as either inoculants or contaminants. The metabolic products of these lactic acid bacteria have considerable effects on the flavor, aroma, and texture of a wine. However, analysis of a wine's microflora, especially the bacteria, is rarely done unless spoilage becomes evident, and identification at the species or strain level is uncommon as the methods required are technically difficult and expensive. In this work, we used Raman spectral fingerprints to discriminate 19 strains of Lactobacillus, Pediococcus, and Oenococcus...
April 24, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28439767/a-bacillus-subtilis-strain-as-probiotic-in-poultry-selection-based-on-in-vitro-functional-properties-and-enzymatic-potentialities
#8
Houda Hmani, Lobna Daoud, Mouna Jlidi, Karim Jalleli, Manel Ben Ali, Adel Hadj Brahim, Mansour Bargui, Alaeddine Dammak, Mamdouh Ben Ali
We have proposed and validate an in vitro probiotic selection, based on enzymatic potentialities associated to well-established probiotic functional properties. A new Bacillus subtilis HB2 isolate, selected based on its high extracellular enzyme production, was chosen as a probiotic candidate for application as animal feed supplement. The HB2 strain showed an excellent acid and bile salts tolerance, a strong adhesion to chick enterocytes and produced antimicrobials against pathogens. An in vivo trial in poultry farming was conducted to evaluate the HB2 probiotic performance...
April 24, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28439766/enhanced-butyric-acid-tolerance-and-production-by-class-i-heat-shock-protein-overproducing-clostridium-tyrobutyricum-atcc-25755
#9
Yukai Suo, Sheng Luo, Yanan Zhang, Zhengping Liao, Jufang Wang
The response of Clostridium tyrobutyricum to butyric acid stress involves various stress-related genes, and therefore overexpression of stress-related genes can improve butyric acid tolerance and yield. Class I heat shock proteins (HSPs) play an important role in the process of protecting bacteria from sudden changes of extracellular stress by assisting protein folding correctly. The results of quantitative real-time PCR indicated that the Class I HSGs grpE, dnaK, dnaJ, groEL, groES, and htpG were significantly upregulated under butyric acid stress, especially the dnaK and groE operons...
April 24, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28429154/developing-elite-neurospora-crassa-strains-for-cellulosic-ethanol-production-using-fungal-breeding
#10
Joshua C Waters, Andrew Nixon, Morgan Dwyer, Justin C Biffinger, Kwangwon Lee
The demand for renewable and sustainable energy has generated considerable interest in the conversion of cellulosic biomass into liquid fuels such as ethanol using a filamentous fungus. While attempts have been made to study cellulose metabolism through the use of knock-out mutants, there have been no systematic effort to characterize natural variation for cellulose metabolism in ecotypes adapted to different habitats. Here, we characterized natural variation in saccharification of cellulose and fermentation in 73 ecotypes and 89 laboratory strains of the model fungus Neurospora crassa...
April 20, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28424881/screening-of-commercial-enzymes-for-poly-ethylene-terephthalate-pet-hydrolysis-and-synergy-studies-on-different-substrate-sources
#11
Aline Machado de Castro, Adriano Carniel, José Nicomedes Junior, Absai da Conceição Gomes, Érika Valoni
Poly(ethylene terephthalate) (PET) is one of the most consumed plastics in the world. The development of efficient technologies for its depolymerization for monomers reuse is highly encouraged, since current recycling rates are still very low. In this study, 16 commercial lipases and cutinases were evaluated for their abilities to catalyze the hydrolysis of two PET samples. Humicola insolens cutinase showed the best performance and was then used in reactions on other PET sources, solely or in combination with the efficient mono(hydroxyethyl terephthalate)-converting lipase from Candida antarctica...
April 19, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28382525/5-aminolevulinic-acid-production-from-inexpensive-glucose-by-engineering-the-c4-pathway-in-escherichia-coli
#12
Wenwen Ding, Huanjiao Weng, Guocheng Du, Jian Chen, Zhen Kang
5-Aminolevulinic acid (ALA), the first committed intermediate for natural biosynthesis of tetrapyrrole compounds, has recently drawn intensive attention due to its broad potential applications. In this study, we describe the construction of recombinant Escherichia coli strains for ALA production from glucose via the C4 pathway. The hemA gene from Rhodobacter capsulatus was optimally overexpressed using a ribosome binding site engineering strategy, which enhanced ALA production substantially from 20 to 689 mg/L...
April 5, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28353181/effects-of-upr-and-erad-pathway-on-the-prolyl-endopeptidase-production-in-pichia-pastoris-by-controlling-of-nitrogen-source
#13
Xiao-Dong Wang, Ting Jiang, Xiao-Wei Yu, Yan Xu
Prolyl endopeptidase (PEP) is very useful in various industries, while the high cost of enzyme production remains a major obstacle for its industrial applications. Pichia pastoris has been used for the PEP production; however, the fermentation process has not be investigated and little is known about the impact of excessive PEP production on the host cell physiology. Here, we optimized the nitrogen source to improve the PEP expression level and further evaluated the cellular response including UPR and ERAD...
March 28, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28332050/rapidly-directional-biotransformation-of-tauroursodeoxycholic-acid-through-engineered-escherichia-coli
#14
Jie Shi, Jie Wang, Lu Yu, Li Yang, Shujuan Zhao, Zhengtao Wang
Bear bile powder is a precious medicinal material. It is characterized by high content of tauroursodeoxycholic acid (TUDCA) at a ratio of 1.0-1.5 to taurochenodeoxycholic acid (TCDCA). Here, we reported the biotransformation of tauroursodeoxycholic acid (TUDCA) through Escherichia coli engineered with a two-step mimic biosynthetic pathway of TUDCA from taurochenodeoxycholic acid (TCDCA). Two 7α-hydroxysteroid dehydrogenase (7α-HSDH) and two 7β-hydroxysteroid dehydrogenase (7β-HSDH) genes (named as α1, α2, β1, and β2) were selected and synthesized to create four pathway variants using ePathBrick...
March 22, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28324236/efficient-de-novo-synthesis-of-resveratrol-by-metabolically-engineered-escherichia-coli
#15
Junjun Wu, Peng Zhou, Xia Zhang, Mingsheng Dong
Resveratrol has been the subject of numerous scientific investigations due to its health-promoting activities against a variety of diseases. However, developing feasible and efficient microbial processes remains challenging owing to the requirement of supplementing expensive phenylpropanoic precursors. Here, various metabolic engineering strategies were developed for efficient de novo biosynthesis of resveratrol. A recombinant malonate assimilation pathway from Rhizobium trifolii was introduced to increase the supply of the key precursor malonyl-CoA and simultaneously, the clustered regularly interspaced short palindromic repeats interference system was explored to down-regulate fatty acid biosynthesis pathway to inactivate the malonyl-CoA consumption pathway...
March 21, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28321646/selective-bio-oxidation-of-propane-to-acetone-using-methane-oxidizing-methylomonas-sp-%C3%A2-dh-1
#16
Dong Hoon Hur, Thu Thi Nguyen, Donghyuk Kim, Eun Yeol Lee
Propane is the major component of liquefied petroleum gas (LPG). Nowadays, the use of LPG is decreasing, and thus utilization of propane as a chemical feedstock is in need of development. An efficient biological conversion of propane to acetone using a methanotrophic whole cell as the biocatalyst was proposed and investigated. A bio-oxidation pathway of propane to acetone in Methylomonas sp. DH-1 was analyzed by gene expression profiling via RNA sequencing. Propane was oxidized to 2-propanol by particulate methane monooxygenase and subsequently to acetone by methanol dehydrogenases...
March 20, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28315963/enhancement-of-2-3-butanediol-production-from-jerusalem-artichoke-tuber-extract-by-a-recombinant-bacillus-sp-strain-brc1-with-increased-inulinase-activity
#17
Jang Min Park, Baek-Rock Oh, In Yeong Kang, Sun-Yeon Heo, Jeong-Woo Seo, Seung-Moon Park, Won-Kyung Hong, Chul Ho Kim
A Bacillus sp. strain named BRC1 is capable of producing 2,3-butanediol (2,3-BD) using hydrolysates of the Jerusalem artichoke tuber (JAT), a rich source of the fructose polymer inulin. To enhance 2,3-BD production, we undertook an extensive analysis of the Bacillus sp. BRC1 genome, identifying a putative gene (sacC) encoding a fructan hydrolysis enzyme and characterizing the activity of the resulting recombinant protein expressed in and purified from Escherichia coli. Introduction of the sacC gene into Bacillus sp...
March 18, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28303352/redirecting-carbon-flux-through-pgi-deficient-and-heterologous-transhydrogenase-toward-efficient-succinate-production-in-corynebacterium-glutamicum
#18
Chen Wang, Zhihui Zhou, Heng Cai, Zhongjun Chen, Hongtao Xu
Corynebacterium glutamicum is particularly known for its potentiality in succinate production. We engineered C. glutamicum for the production of succinate. To enhance C3-C4 carboxylation efficiency, chromosomal integration of the pyruvate carboxylase gene pyc resulted in strain NC-4. To increase intracellular NADH pools, the pntAB gene from Escherichia coli, encoding for transhydrogenase, was chromosomally integrated into NC-4, leading to strain NC-5. Furthermore, we deleted pgi gene in strain NC-5 to redirect carbon flux to the pentose phosphate pathway (PPP)...
March 16, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28289902/discovery-of-synthesis-and-secretion-of-polyol-esters-of-fatty-acids-by-four-basidiomycetous-yeast-species-in-the-order-sporidiobolales
#19
Luis A Garay, Irnayuli R Sitepu, Tomas Cajka, Oliver Fiehn, Erin Cathcart, Russell W Fry, Atit Kanti, Agustinus Joko Nugroho, Sarah Asih Faulina, Sira Stephanandra, J Bruce German, Kyria L Boundy-Mills
Polyol esters of fatty acids (PEFA) are amphiphilic glycolipids produced by yeast that could play a role as natural, environmentally friendly biosurfactants. We recently reported discovery of a new PEFA-secreting yeast species, Rhodotorula babjevae, a basidiomycetous yeast to display this behavior, in addition to a few other Rhodotorula yeasts reported on the 1960s. Additional yeast species within the taxonomic order Sporidiobolales were screened for secreted glycolipid production. PEFA production equal or above 1 g L(-1) were detected in 19 out of 65 strains of yeast screened, belonging to 6 out of 30 yeast species tested...
March 13, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28285402/a-semi-empirical-glycosylation-model-of-a-camelid-monoclonal-antibody-under-hypothermia-cell-culture-conditions
#20
Hengameh Aghamohseni, Maureen Spearman, Kaveh Ohadi, Katrin Braasch, Murray Moo-Young, Michael Butler, Hector M Budman
The impact of cell culture environment on the glycan distribution of a monoclonal antibody (mAb) has been investigated through a combination of experiments and modeling. A newly developed CHO DUXB cell line was cultivated at two levels of initial Glutamine (Gln) concentrations (0, 4 mM) and incubation temperatures of (33 and 37 °C) in batch operation mode. Hypothermia was applied either through the entire culture duration or only during the post-exponential phase. Beyond reducing cell growth and increasing productivity, hypothermia significantly altered the galactosylation index profiles as compared to control conditions...
March 11, 2017: Journal of Industrial Microbiology & Biotechnology
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