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Journal of Industrial Microbiology & Biotechnology

Yiyi Zhong, Xi Lu, Lei Xing, Shiu Woon Allen Ho, Hoi Shan Kwan
The filamentous fungus Aspergillus oryzae is used in soy sauce koji making due to its high productivity of hydrolytic enzymes. In this study, we compared the genomes and transcriptomes of an industrial strain RD2 and a strain with decreased fermentation performance TS2, aiming to explain their phenotypic differences at the molecular level. Under the regulation of conidiation and fermentation conditions, the enhanced hydrolytic enzyme production and flavor precursor formation in RD2 described a complete expression profile necessary to maintain desirable fermentation performance...
July 5, 2018: Journal of Industrial Microbiology & Biotechnology
T S Oberg, J L Steele, S C Ingham, V V Smeianov, E P Briczinski, A Abdalla, J R Broadbent
In the published article, the co-author Abdelmoneim Abdalla's affiliation has been published incompletely. The additional affiliation is given below.
June 29, 2018: Journal of Industrial Microbiology & Biotechnology
Xue Lin, Cui-Ying Zhang, Lu Meng, Xiao-Wen Bai, Dong-Guang Xiao
Maltose metabolism of baker's yeast (Saccharomyces cerevisiae) in lean dough is suppressed by the glucose effect, which negatively affects dough fermentation. In this study, differences and interactions among SNF4 (encoding for the regulatory subunit of Snf1 kinase) overexpression and REG1 and REG2 (which encodes for the regulatory subunits of the type I protein phosphatase) deletions in maltose metabolism of baker's yeast were investigated using various mutants. Results revealed that SNF4 overexpression and REG1 and REG2 deletions effectively alleviated glucose repression at different levels, thereby enhancing maltose metabolism and leavening ability to varying degrees...
June 23, 2018: Journal of Industrial Microbiology & Biotechnology
Leonard Katz, Yvonne Y Chen, Ramon Gonzalez, Todd C Peterson, Huimin Zhao, Richard H Baltz
Synthetic biology is a logical extension of what has been called recombinant DNA (rDNA) technology or genetic engineering since the 1970s. As rDNA technology has been the driver for the development of a thriving biotechnology industry today, starting with the commercialization of biosynthetic human insulin in the early 1980s, synthetic biology has the potential to take the industry to new heights in the coming years. Synthetic biology advances have been driven by dramatic cost reductions in DNA sequencing and DNA synthesis; by the development of sophisticated tools for genome editing, such as CRISPR/Cas9; and by advances in informatics, computational tools, and infrastructure to facilitate and scale analysis and design...
June 18, 2018: Journal of Industrial Microbiology & Biotechnology
Kristina M Mahan, Rosemary K Le, Tyrone Wells, Seth Anderson, Joshua S Yuan, Ryan J Stoklosa, Aditya Bhalla, David B Hodge, Arthur J Ragauskas
Livestock and fish farming are rapidly growing industries facing the simultaneous pressure of increasing production demands and limited protein required to produce feed. Bacteria that can convert low-value non-food waste streams into singe cell protein (SCP) present an intriguing route for rapid protein production. The oleaginous bacterium Rhodococcus opacus serves as a model organism for understanding microbial lipid production. SCP production has not been explored using an organism from this genus. In the present research, R...
June 18, 2018: Journal of Industrial Microbiology & Biotechnology
Ying Xia, Lirong Yang, Liming Xia
The industrial application of Trichoderma reesei has been greatly limited by insufficient β-glucosidase activity in its cellulase system. In this study, a novel β-glucosidase expression cassette was constructed and integrated at the target site in T. reesei ZU-02, which achieved the overexpression of β-glucosidase gene and in situ disruption of the cellulase transcriptional repressor ACE1. The resulting transformants showed significant increase in both β-glucosidase activity (BGA) and filter paper activity (FPA)...
June 16, 2018: Journal of Industrial Microbiology & Biotechnology
Ping Song, Kai Yuan, Tingting Qin, Ke Zhang, Xiao-Jun Ji, Lujing Ren, Rongfeng Guan, Jianping Wen, He Huang
Metabolic profiling was used to discover mechanisms of increased pneumocandin B0 production in a high-yield strain by comparing it with its parent strain. Initially, 79 intracellular metabolites were identified, and the levels of 15 metabolites involved in six pathways were found to be directly correlated with pneumocandin B0 biosynthesis. Then by combining the analysis of key enzymes, acetyl-CoA and NADPH were identified as the main factors limiting pneumocandin B0 biosynthesis. Other metabolites, such as pyruvate, α-ketoglutaric acid, lactate, unsaturated fatty acids and previously unreported metabolite γ-aminobutyric acid were shown to play important roles in pneumocandin B0 biosynthesis and cell growth...
June 14, 2018: Journal of Industrial Microbiology & Biotechnology
Xiangbin Chen, Linping Yu, Guanqing Qiao, Guo-Qiang Chen
Halomonas spp. are able to grow under a high salt concentration at alkali pH, they are able to resist contamination by other microbes. Development of Halomonas spp. as platform production strains for the next-generation industrial biotechnology (NGIB) is intensively studied. Among Halomonas spp., Halomonas bluephagenesis is the best studied one with available engineering tools and methods to reprogram it for production of various polyhydroxyalkanoates, proteins, and chemicals. Due to its contamination resistance, H...
June 8, 2018: Journal of Industrial Microbiology & Biotechnology
Lijie Cao, Dongmei Liang, Panlong Hao, Qianqian Song, Ershu Xue, Qinggele Caiyin, Zihao Cheng, Jianjun Qiao
Cell wall is closely related to bacterial robustness and adsorption capacity, playing crucial roles in nisin production in Lactococcus lactis. Peptidoglycan (PG), the essential component of cell wall, is usually modified with MurNAc O-acetylation and GlcNAc N-deacetylation, catalyzed by YvhB and XynD, respectively. In this study, increasing the two modifications in L. lactis F44 improved autolysis resistance by decreasing the susceptibility to PG hydrolases. Furthermore, both modifications were positively associated with overall cross-linkage, contributing to cell wall integrity...
June 6, 2018: Journal of Industrial Microbiology & Biotechnology
Victor E Balderas-Hernández, Kevin Correia, Radhakrishnan Mahadevan
Toxic concentrations of monocarboxylic weak acids present in lignocellulosic hydrolyzates affect cell integrity and fermentative performance of Saccharomyces cerevisiae. In this work, we report the deletion of the general catabolite repressor Mig1p as a strategy to improve the tolerance of S. cerevisiae towards inhibitory concentrations of acetic, formic or levulinic acid. In contrast with the wt yeast, where the growth and ethanol production were ceased in presence of acetic acid 5 g/L or formic acid 1.75 g/L (initial pH not adjusted), the m9 strain (Δmig1::kan) produced 4...
June 6, 2018: Journal of Industrial Microbiology & Biotechnology
Hugo Ramírez-Aldaba, Jorge Vázquez-Arenas, Fabiola S Sosa-Rodríguez, Donato Valdez-Pérez, Estela Ruiz-Baca, Gabriel Trejo-Córdoba, Miguel A Escobedo-Bretado, Luis Lartundo-Rojas, Patricia Ponce-Peña, René H Lara
Chemical and surface analyses are carried out using Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM-EDS), atomic force microscopy (AFM), confocal laser scanning microscopy (CLSM), glow discharge spectroscopy (GDS) and extracellular surface protein quantification to thoroughly investigate the effect of supplementary As(V) during biooxidation of arsenopyrite by Acidithiobacillus thiooxidans. It is revealed that arsenic can enhance bacterial reactions during bioleaching, which can strongly influence its mobility...
June 1, 2018: Journal of Industrial Microbiology & Biotechnology
Joseph Groom, Daehwan Chung, Sun-Ki Kim, Adam Guss, Janet Westpheling
A limitation to the engineering of cellulolytic thermophiles is the availability of functional, thermostable (≥ 60 °C) replicating plasmid vectors for rapid expression and testing of genes that provide improved or novel fuel molecule production pathways. A series of plasmid vectors for genetic manipulation of the cellulolytic thermophile Caldicellulosiruptor bescii has recently been extended to Clostridium thermocellum, another cellulolytic thermophile that very efficiently solubilizes plant biomass and produces ethanol...
May 28, 2018: Journal of Industrial Microbiology & Biotechnology
Shaoxiong Liang, Dacheng Gao, Huanhuan Liu, Cheng Wang, Jianping Wen
As an important feedstock monomer for the production of biodegradable stereo-complex poly-lactic acid polymer, D-lactate has attracted much attention. To improve D-lactate production by microorganisms such as Lactobacillus delbrueckii, various fermentation conditions were performed, such as the employment of anaerobic fermentation, the utilization of more suitable neutralizing agents, and exploitation of alternative nitrogen sources. The highest D-lactate titer could reach 133 g/L under the optimally combined fermentation condition, increased by 70...
May 28, 2018: Journal of Industrial Microbiology & Biotechnology
Sumit Joshi, Shweta Goyal, M Sudhakara Reddy
Microbial-induced carbonate precipitation (MICP) has a potential to improve the durability properties and remediate cracks in concrete. In the present study, the main emphasis is placed upon replacing the expensive laboratory nutrient broth (NB) with corn steep liquor (CSL), an industrial by-product, as an alternate nutrient medium during biocementation. The influence of organic nutrients (carbon and nitrogen content) of CSL and NB on the chemical and structural properties of concrete structures is studied...
May 28, 2018: Journal of Industrial Microbiology & Biotechnology
Huadong Peng, Lizhong He, Victoria S Haritos
Microbially produced lipids have attracted attention for their environmental benefits and commercial value. We have combined lipid pathway engineering in Saccharomyces cerevisiae yeast with bioprocess design to improve productivity and explore barriers to enhanced lipid production. Initially, individual gene expression was tested for impact on yeast growth and lipid production. Then, two base strains were prepared for enhanced lipid accumulation and stabilization steps by combining DGAT1, ΔTgl3 with or without Atclo1, which increased lipid content ~ 1...
May 26, 2018: Journal of Industrial Microbiology & Biotechnology
Jin-Ying Guo, Kun-Le Hu, Chang-Hao Bi, Qing-Yan Li, Xue-Li Zhang
Glycerol, which is an inevitable by-product of biodiesel production, is an ideal carbon source for the production of carotenoids due to its low price, good availability and chemically reduced status, which results in a low requirement for additional reducing equivalents. In this study, an alternative carbon-utilization pathway was constructed in Escherichia coli to enable more efficient β-carotene production from glycerol. An aldehyde reductase gene (alrd) and an aldehyde dehydrogenase gene (aldH) from Ralstonia eutropha H16 were integrated into the E...
May 11, 2018: Journal of Industrial Microbiology & Biotechnology
Jie Cheng, Yuding Huang, Le Mi, Wujiu Chen, Dan Wang, Qinhong Wang
Deficiency in petroleum resources and increasing environmental concerns have pushed a bio-based economy to be built, employing a highly reproducible, metal contaminant free, sustainable and green biomanufacturing method. Here, a chiral drug intermediate L-pipecolic acid has been synthesized from biomass-derived lysine. This artificial bioconversion system involves the coexpression of four functional genes, which encode L-lysine α-oxidase from Scomber japonicus, glucose dehydrogenase from Bacillus subtilis, Δ1 -piperideine-2-carboxylase reductase from Pseudomonas putida, and lysine permease from Escherichia coli...
May 10, 2018: Journal of Industrial Microbiology & Biotechnology
Huifang Cao, Yueqi Zhang, Pengjun Shi, Rui Ma, Hong Yang, Wei Xia, Ying Cui, Huiying Luo, Yingguo Bai, Bin Yao
In the feed industry, β-glucosidase has been widely used in the conversion of inactive and bounded soybean isoflavones into active aglycones. However, the conversion is frequently inhibited by the high concentration of intestinal glucose in monogastric animals. In this study, a GH1 β-glucosidase (AsBG1) with high specific activity, thermostability and glucose tolerance (IC 50  = 800 mM) was identified. It showed great glucose tolerance against substrates with hydrophobic aryl ligands (such as pNPG and soy isoflavones)...
May 9, 2018: Journal of Industrial Microbiology & Biotechnology
Jason Fontana, William E Voje, Jesse G Zalatan, James M Carothers
Dynamic control of gene expression is emerging as an important strategy for controlling flux in metabolic pathways and improving bioproduction of valuable compounds. Integrating dynamic genetic control tools with CRISPR-Cas transcriptional regulation could significantly improve our ability to fine-tune the expression of multiple endogenous and heterologous genes according to the state of the cell. In this mini-review, we combine an analysis of recent literature with examples from our own work to discuss the prospects and challenges of developing dynamically regulated CRISPR-Cas transcriptional control systems for applications in synthetic biology and metabolic engineering...
May 8, 2018: Journal of Industrial Microbiology & Biotechnology
XingYa Zhao, JianYong Xu, Ming Tan, ZhenXiao Yu, ShiBin Yang, HongChen Zheng, Hui Song
Bacillus sp. strains as attractive hosts for the production of heterologous secretory proteins usually play important roles in bio-industry. However, low transformation efficiency of exogenous plasmids limited the application of Bacillus species. Here, a novel plasmid interspecific transfer system, with high transformation efficiency, high positive rate, and convenient manipulation, has been successfully constructed. A high electrotransformation efficiency strain Bacillus subtilis F-168 containing the counter-selectable marker mazF was used as the plasmid donor strain in this transfer method...
May 8, 2018: Journal of Industrial Microbiology & Biotechnology
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