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Journal of Industrial Microbiology & Biotechnology

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https://www.readbyqxmd.com/read/28646288/extremophilic-proteases-as-novel-and-efficient-tools-in-short-peptide-synthesis
#1
REVIEW
Aneta M Białkowska, Krzysztof Morawski, Tomasz Florczak
The objective of this review is to outline the crucial role that peptides play in various sectors, including medicine. Different ways of producing these compounds are discussed with an emphasis on the benefits offered by industrial enzyme biotechnology. This paper describes mechanisms of peptide bond formation using a range of proteases with different active site structures. Importantly, these enzymes may be further improved chemically and/or genetically to make them better suited for their various applications and process conditions...
June 23, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28639077/microbial-compositions-and-metabolic-interactions-in-one-and-two-phase-partitioning-airlift-bioreactors-treating-a-complex-voc-mixture
#2
Chao Wu, Peilun Xu, Yinfeng Xia, Wei Li, Sujing Li, Xiangqian Wang
Engineered microbial ecosystems in bioscrubbers for the treatment of volatile organic compounds (VOCs) have been complicated by complex VOC mixtures from various industrial emissions. Microbial associations with VOC removal performance of the bioscrubbers are still not definitive. Here, one- and two-phase partitioning airlift bioreactors were used for the treatment of a complex VOC mixture. Microbial characteristics in both bioreactors were uncovered by high-throughput metagenomics sequencing. Results showed that dominant species with specialized VOC biodegradability were mainly responsible for high removal efficiency of relative individual VOC...
June 21, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28567672/bioprocess-engineering-to-produce-9-nonanoyloxy-nonanoic-acid-by-a-recombinant-corynebacterium-glutamicum-based-biocatalyst
#3
Hyeonsoo Kim, Soohyun Park, Sukhyeong Cho, Jeongmo Yang, Kijun Jeong, Jinbyung Park, Jinwon Lee
Here, Corynebacterium glutamicum ATCC13032 expressing Baeyer-Villiger monooxygenase from Pseudomonas putida KT2440 was designed to produce 9-(nonanoyloxy) nonanoic acid from 10-ketostearic acid. Diverse parameters including cultivation and reaction temperatures, type of detergent, and pH were found to improve biotransformation efficiency. The optimal temperature of cultivation for the production of 9-(nonanoyloxy) nonanoic acid from 10-ketostearic acid using whole cells of recombinant C. glutamicum was 15 °C, but the reaction temperature was optimal at 30 °C...
June 1, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28555391/improved-recovery-and-biological-activities-of-an-engineered-polyene-npp-analogue-in-pseudonocardia-autotrophica
#4
Hyung-Jin Won, Hye-Jin Kim, Jin-Young Jang, Seung-Hoon Kang, Si-Sun Choi, Eung-Soo Kim
NPP A1 produced by Pseudonocardia autotrophica is a unique disaccharide-containing polyene macrolide. NPP A1 was reported to have higher water solubility and lower hemolytic toxicity than nystatin A1 while retaining its antifungal activity. An engineered NPP A1 analogue, NPP A2, was generated by inactivation of the nppL gene, encoding a P450 monooxygenase in P. autotrophica. The resulting compound exhibited the corresponding chemical structure of NPP A1 but lacked a C10 hydroxyl group. In this study, newly developed crystallization recovery methods for NPP A2 purification, followed by an evaluation of in vitro antifungal activity and hemolytic activity, were performed...
May 29, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28551747/damage-to-the-microbial-cell-membrane-during-pyrolytic-sugar-utilization-and-strategies-for-increasing-resistance
#5
Tao Jin, Marjorie R Rover, Elspeth M Petersen, Zhanyou Chi, Ryan G Smith, Robert C Brown, Zhiyou Wen, Laura R Jarboe
Lignocellulosic biomass is an appealing feedstock for the production of biorenewable fuels and chemicals, and thermochemical processing is a promising method for depolymerizing it into sugars. However, trace compounds in this pyrolytic sugar syrup are inhibitory to microbial biocatalysts. This study demonstrates that hydrophobic inhibitors damage the cell membrane of ethanologenic Escherichia coli KO11+lgk. Adaptive evolution was employed to identify design strategies for improving pyrolytic sugar tolerance and utilization...
May 27, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28547323/a-shortened-two-enzyme-pathway-for-2-3-butanediol-production-in-escherichia-coli
#6
Shamlan M S Reshamwala, Shalini S Deb, Arvind M Lali
The platform chemical 2,3-butanediol (2,3-BDO) is produced by a number of microorganisms via a three-enzyme pathway starting from pyruvate. Here, we report production of 2,3-BDO via a shortened, two-enzyme pathway in Escherichia coli. A synthetic operon consisting of the acetolactate synthase (ALS) and acetoin reductase (AR) genes from Enterobacter under control of the T7 promoter was cloned in an episomal plasmid. E. coli transformed with this plasmid produced 2,3-BDO and the pathway intermediate acetoin, demonstrating that the shortened pathway was functional...
May 25, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28547322/metabolic-engineering-of-saccharomyces-cerevisiae-for-production-of-germacrene-a-a-precursor-of-beta-elemene
#7
Yating Hu, Yongjin J Zhou, Jichen Bao, Luqi Huang, Jens Nielsen, Anastasia Krivoruchko
Beta-elemene, a sesquiterpene and the major component of the medicinal herb Curcuma wenyujin, has antitumor activity against various types of cancer and could potentially serve as a potent antineoplastic drug. However, its current mode of production through extraction from plants has been inefficient and suffers from limited natural resources. Here, we engineered a yeast cell factory for the sustainable production of germacrene A, which can be transformed to beta-elemene by a one-step chemical reaction in vitro...
May 25, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28536841/fed-batch-hydrolysate-addition-and-cell-separation-by-settling-in-high-cell-density-lignocellulosic-ethanol-fermentations-on-afex%C3%A2-corn-stover-in-the-rapid-bioconversion-with-integrated-recycling-technology-process
#8
Cory Sarks, Mingjie Jin, Venkatesh Balan, Bruce E Dale
The Rapid Bioconversion with Integrated recycling Technology (RaBIT) process uses enzyme and yeast recycling to improve cellulosic ethanol production economics. The previous versions of the RaBIT process exhibited decreased xylose consumption using cell recycle for a variety of different micro-organisms. Process changes were tested in an attempt to eliminate the xylose consumption decrease. Three different RaBIT process changes were evaluated in this work including (1) shortening the fermentation time, (2) fed-batch hydrolysate addition, and (3) selective cell recycling using a settling method...
May 23, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28536840/insights-from-the-complete-genome-sequence-of-clostridium-tyrobutyricum-provide-a-platform-for-biotechnological-and-industrial-applications
#9
Qian Wu, Tingting Liu, Liying Zhu, He Huang, Ling Jiang
Genetic research enables the evolution of novel biochemical reactions for the production of valuable chemicals from environmentally-friendly raw materials. However, the choice of appropriate microorganisms to support these reactions, which must have strong robustness and be capable of a significant product output, is a major difficulty. In the present study, the complete genome of the Clostridium tyrobutyricum strain CCTCC W428, a hydrogen- and butyric acid-producing bacterium with increased oxidative tolerance was analyzed...
May 23, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28509952/high-yield-production-of-mannitol-by-leuconostoc-pseudomesenteroides-ctcc-g123-from-chicory-derived-inulin-hydrolysate
#10
Min Zhang, Lei Gu, Chao Cheng, Junru Zhu, Hao Wu, Jiangfeng Ma, Weiliang Dong, Xiangping Kong, Min Jiang, Pingkai Ouyang
Chicory is an agricultural plant with considerable potential as a carbohydrate substrate for low-cost production of biochemicals. In this work, the production of mannitol by Leuconostoc pseudomesenteroides CTCC G123 from chicory-derived inulin hydrolysate was investigated. The bioconversion process initially suffered from the leakage of fructose to the phosphoketolase pathway, resulting in a low mannitol yield. When inulin hydrolysate was supplemented with glucose as a substrate for mannitol production in combination with aeration induction and nicotinic acid induced redox modulation strategies, the mannitol yield greatly improved...
May 16, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28508118/effects-of-aeration-on-metabolic-profiles-of-mortierella-alpina-during-the-production-of-arachidonic-acid
#11
Xin Zhang, Ling Jiang, Li-Ying Zhu, Qin-Ke Shen, Xiao-Jun Ji, He Huang, Hong-Man Zhang
To investigate the metabolic regulation against oxygen supply, comparative metabolomics was performed to explore the metabolic responses of Mortierella alpina in the process of arachidonic acid (ARA) production. More than 110 metabolites involved in Embden-Meyerhof-Parnas pathway, pentose phosphate pathway, tricarboxylic acid cycle, inositol phosphate metabolism, fatty acid biosynthesis, and amino acid metabolism were identified by gas chromatography-mass spectrometry. Samples at different aeration rates were clearly distinguished by principal components analysis and partial least squares analysis, indicating that oxygen supply had a profound effect on the metabolism of M...
May 15, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28497282/highly-complex-substrates-lead-to-dynamic-bacterial-community-for-polyhydroxyalkanoates-production
#12
Diogo Queirós, Alexandre Fonseca, Simona Rossetti, Luísa S Serafim, Paulo C Lemos
Mixed microbial cultures (MMC) and waste/surplus substrates, as hardwood spent sulfite liquor, are being used to decrease polyhydroxyalkanoates' (PHA) production costs. The process involves two or three steps, being the selection step a crucial one. For the industrial implementation of this strategy, reactor stability in terms of both performance and microbial community presence has to be considered. A long-term operation of a sequencing batch reactor under feast/famine conditions was performed along with microbial community identification/quantification using FISH and DGGE...
May 11, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28451838/improved-cider-fermentation-performance-and-quality-with-newly-generated-saccharomyces-cerevisiae%C3%A2-%C3%A3-%C3%A2-saccharomyces-eubayanus-hybrids
#13
Frederico Magalhães, Kristoffer Krogerus, Virve Vidgren, Mari Sandell, Brian Gibson
Yeast cryotolerance may be advantageous for cider making, where low temperatures are usually employed. Here, we crossed the cryotolerant S. eubayanus with a S. cerevisiae wine strain and assessed the suitability of the hybrids for low-temperature cider fermentation. All strains fermented the juice to 5% ABV, but at different rates; hybrid strains outperformed S. cerevisiae, which was sensitive to low temperatures. The best hybrid fermented similarly to S. eubayanus. S. eubayanus produced sulphurous off flavours which masked a high concentration of fruity ester notes...
April 27, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28451837/high-level-productivity-of-%C3%AE-%C3%AF-dodecanedioic-acid-with-a-newly-isolated-candida-viswanathii-strain
#14
Weifeng Cao, Hongbao Li, Jianquan Luo, Junxiang Yin, Yinhua Wan
α,ω-Dicarboxylic acids (DC) are versatile chemical intermediates with different chain lengths, which are well-known as polymer building block. In this work, a new strain with high productivity of DC was isolated from oil-contaminated soil. Based on the morphology and phylogenetic analyses of the internal transcribed spacer sequences, it was characterized as Candida viswanathii. It was found that the contribution of carbon flux to the cell growth and DC production from n-dodecane could be regulated by the sucrose and yeast extract concentrations in the medium, and besides the broth pH, a suitable proportioning of sucrose and yeast extract was the key to achieve the optimal transition from cell growth phase to DC production phase...
April 27, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28444480/model-based-biotechnological-potential-analysis-of-kluyveromyces-marxianus-central-metabolism
#15
A Pentjuss, E Stalidzans, J Liepins, A Kokina, J Martynova, P Zikmanis, I Mozga, R Scherbaka, H Hartman, M G Poolman, D A Fell, A Vigants
The non-conventional yeast Kluyveromyces marxianus is an emerging industrial producer for many biotechnological processes. Here, we show the application of a biomass-linked stoichiometric model of central metabolism that is experimentally validated, and mass and charge balanced for assessing the carbon conversion efficiency of wild type and modified K. marxianus. Pairs of substrates (lactose, glucose, inulin, xylose) and products (ethanol, acetate, lactate, glycerol, ethyl acetate, succinate, glutamate, phenylethanol and phenylalanine) are examined by various modelling and optimisation methods...
April 25, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28439768/discrimination-of-wine-lactic-acid-bacteria-by-raman-spectroscopy
#16
Susan B Rodriguez, Mark A Thornton, Roy J Thornton
Species of Lactobacillus, Pediococcus, Oenococcus, and Leuconostoc play an important role in winemaking, as either inoculants or contaminants. The metabolic products of these lactic acid bacteria have considerable effects on the flavor, aroma, and texture of a wine. However, analysis of a wine's microflora, especially the bacteria, is rarely done unless spoilage becomes evident, and identification at the species or strain level is uncommon as the methods required are technically difficult and expensive. In this work, we used Raman spectral fingerprints to discriminate 19 strains of Lactobacillus, Pediococcus, and Oenococcus...
April 24, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28439767/a-bacillus-subtilis-strain-as-probiotic-in-poultry-selection-based-on-in-vitro-functional-properties-and-enzymatic-potentialities
#17
Houda Hmani, Lobna Daoud, Mouna Jlidi, Karim Jalleli, Manel Ben Ali, Adel Hadj Brahim, Mansour Bargui, Alaeddine Dammak, Mamdouh Ben Ali
We have proposed and validate an in vitro probiotic selection, based on enzymatic potentialities associated to well-established probiotic functional properties. A new Bacillus subtilis HB2 isolate, selected based on its high extracellular enzyme production, was chosen as a probiotic candidate for application as animal feed supplement. The HB2 strain showed an excellent acid and bile salts tolerance, a strong adhesion to chick enterocytes and produced antimicrobials against pathogens. An in vivo trial in poultry farming was conducted to evaluate the HB2 probiotic performance...
April 24, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28439766/enhanced-butyric-acid-tolerance-and-production-by-class-i-heat-shock-protein-overproducing-clostridium-tyrobutyricum-atcc-25755
#18
Yukai Suo, Sheng Luo, Yanan Zhang, Zhengping Liao, Jufang Wang
The response of Clostridium tyrobutyricum to butyric acid stress involves various stress-related genes, and therefore overexpression of stress-related genes can improve butyric acid tolerance and yield. Class I heat shock proteins (HSPs) play an important role in the process of protecting bacteria from sudden changes of extracellular stress by assisting protein folding correctly. The results of quantitative real-time PCR indicated that the Class I HSGs grpE, dnaK, dnaJ, groEL, groES, and htpG were significantly upregulated under butyric acid stress, especially the dnaK and groE operons...
April 24, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28429154/developing-elite-neurospora-crassa-strains-for-cellulosic-ethanol-production-using-fungal-breeding
#19
Joshua C Waters, Andrew Nixon, Morgan Dwyer, Justin C Biffinger, Kwangwon Lee
The demand for renewable and sustainable energy has generated considerable interest in the conversion of cellulosic biomass into liquid fuels such as ethanol using a filamentous fungus. While attempts have been made to study cellulose metabolism through the use of knock-out mutants, there have been no systematic effort to characterize natural variation for cellulose metabolism in ecotypes adapted to different habitats. Here, we characterized natural variation in saccharification of cellulose and fermentation in 73 ecotypes and 89 laboratory strains of the model fungus Neurospora crassa...
April 20, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28424881/screening-of-commercial-enzymes-for-poly-ethylene-terephthalate-pet-hydrolysis-and-synergy-studies-on-different-substrate-sources
#20
Aline Machado de Castro, Adriano Carniel, José Nicomedes Junior, Absai da Conceição Gomes, Érika Valoni
Poly(ethylene terephthalate) (PET) is one of the most consumed plastics in the world. The development of efficient technologies for its depolymerization for monomers reuse is highly encouraged, since current recycling rates are still very low. In this study, 16 commercial lipases and cutinases were evaluated for their abilities to catalyze the hydrolysis of two PET samples. Humicola insolens cutinase showed the best performance and was then used in reactions on other PET sources, solely or in combination with the efficient mono(hydroxyethyl terephthalate)-converting lipase from Candida antarctica...
April 19, 2017: Journal of Industrial Microbiology & Biotechnology
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