Read by QxMD icon Read

Journal of Microbiology / the Microbiological Society of Korea

Matt N Hicks, Sanjiva Gunasekara, Jose Serate, Jin Park, Pegah Mosharaf, Yue Zhou, Jin-Won Lee, Hwan Youn
The Escherichia coli cAMP receptor protein (CRP) utilizes the helix-turn-helix motif for DNA binding. The CRP's recognition helix, termed F-helix, includes a stretch of six amino acids (Arg180, Glu181, Thr182, Val183, Gly184, and Arg185) for direct DNA contacts. Arg180, Glu181 and Arg185 are known as important residues for DNA binding and specificity, but little has been studied for the other residues. Here we show that Gly184 is another F-helix residue critical for the transcriptional activation function of CRP...
October 2017: Journal of Microbiology / the Microbiological Society of Korea
Mohamed El-Agamy Farh, Yeon-Ju Kim, Priyanka Singh, Sun Young Jung, Jong-Pyo Kang, Deok-Chun Yang
Gram-staining-negative, uniflagellated, rod-shaped, designated as DCY110(T), was isolated from sludge located in Gangwon province, Republic of Korea. The phylogenetic tree of 16S rRNA gene sequence showed that the strain DCY110(T) belonged to the genus Rhodoferax with a close similarity to Rhodoferax saidenbachensis DSM 22694(T) (97.7%), Rhodoferax antarcticus DSM 24876(T) (97.5%), Rhodoferax ferrireducens DSM 15236(T) (97.3%), and Rhodoferax fermentans JCM 7819(T) (96.7%). The predominant isoprenoid quinine was ubiquinone (Q-8)...
October 2017: Journal of Microbiology / the Microbiological Society of Korea
Ki Bum Ahn, A Reum Kim, Kee-Yeon Kum, Cheol-Heui Yun, Seung Hyun Han
Streptococcus mutans is a major etiologic agent of human dental caries that forms biofilms on hard tissues in the human oral cavity, such as tooth and dentinal surfaces. Human β-defensin-3 (HBD3) is a 45-amino-acid natural antimicrobial peptide that has broad spectrum antimicrobial activity against bacteria and fungi. A synthetic peptide consisting of the C-terminal 15 amino acids of HBD3 (HBD3-C15) was recently shown to be sufficient for its antimicrobial activity. Thus, clinical applications of this peptide have garnered attention...
October 2017: Journal of Microbiology / the Microbiological Society of Korea
Yulan Qiu, Xuemei Zhang, Hong Wang, Xinyuan Zhang, Yunjun Mo, Xiaoyu Sun, Jichao Wang, Yibing Yin, Wenchun Xu
Streptococcus pneumoniae is a leading cause of infectious diseases in children under 5-year-old. Vaccine has been used as an indispensable strategy to prevent S. pneumoniae infection for more than 30 years. Our previous studies confirmed that mucosal immunization with live attenuated strain SPY1 can protect mice against nasopharyngeal colonization of S. pneumoniae and lethal pneumococcal infection, and the protective effects are comparable with those induced by commercially available 23-valent polysaccharide vaccine...
October 2017: Journal of Microbiology / the Microbiological Society of Korea
Juan David Ospina-Villa, Nancy Guillén, Cesar Lopez-Camarillo, Jacqueline Soto-Sanchez, Esther Ramirez-Moreno, Raul Garcia-Vazquez, Carlos A Castañon-Sanchez, Abigail Betanzos, Laurence A Marchat
The 25 kDa subunit of the Clevage Factor Im (CFIm25) is an essential factor for messenger RNA polyadenylation in human cells. Therefore, here we investigated whether the homologous protein of Entamoeba histolytica, the protozoan responsible for human amoebiasis, might be considered as a biochemical target for parasite control. Trophozoites were cultured with bacterial double-stranded RNA molecules targeting the EhCFIm25 gene, and inhibition of mRNA and protein expression was confirmed by RT-PCR and Western blot assays, respectively...
October 2017: Journal of Microbiology / the Microbiological Society of Korea
Hae-In Jung, Yun-Jung Kim, Yun-Jung Lee, Hee-Soo Lee, Jung-Kee Lee, Soo-Ki Kim
Burkholderia sp. is a gram-negative bacterium that commonly exists in the environment, and can cause diseases in plants, animals, and humans. Here, a transposon mutant library of a Burkholderia lata isolate from a pig with swine respiratory disease in Korea was screened for strains showing attenuated virulence in Caenorhabditis elegans. One such mutant was obtained, and the Tn5 insertion junction was mapped to rpfR, a gene encoding a cyclic di-GMP phosphodiesterase that functions as a receptor. Mutation of rpfR caused a reduction in growth on CPG agar and swimming motility as well as a rough colony morphology on Congo red agar...
October 2017: Journal of Microbiology / the Microbiological Society of Korea
David P E Crabtree, Brandon J Herrera, Sanghoon Kang
Cell phones and electronic appliances and devices are inseparable from most people in modern society and the electromagnetic field (EMF) from the devices is a potential health threat. Although the direct health effect of a cell phone and its radiofrequency (RF) EMF to human is still elusive, the effect to unicellular organisms is rather apparent. Human microbiota, including skin microbiota, has been linked to a very significant role in the health of a host human body. It is important to understand the response of human skin microbiota to the RF-EMF from cell phones and personal electronic devices, since this may be one of the potential mechanisms of a human health threat brought about by the disruption of the intimate and balanced host-microbiota relationship...
October 2017: Journal of Microbiology / the Microbiological Society of Korea
Li-Xin Lu, Fang-Jie Yao, Peng Wang, Ming Fang, You-Min Zhang, Wei-Tong Zhang, Xiang-Hui Kong, Jia Lu
Auricularia auricula-judae is a traditional edible fungus that is cultivated widely in China. In this study, a genetic linkage map for A. auricula-judae was constructed using a mapping population consisting of 138 monokaryons derived from a hybrid strain (A119-5). The monokaryotic parent strains A14-5 and A18-119 were derived from two cultivated varieties, A14 (Qihei No. 1) and A18 (Qihei No. 2), respectively. In total, 130 simple sequence repeat markers were mapped. These markers were developed using the whole genome sequence of A...
October 2017: Journal of Microbiology / the Microbiological Society of Korea
Ngoc-Loi Nguyen, Woon-Jong Yu, Hye-Young Yang, Jong-Geol Kim, Man-Young Jung, Soo-Je Park, Seong-Woon Roh, Sung-Keun Rhee
Aerobic methane oxidation is a key process in the global carbon cycle that acts as a major sink of methane. In this study, we describe a novel methanotroph designated EMGL16-1 that was isolated from a freshwater lake using the floating filter culture technique. Based on a phylogenetic analysis of 16S rRNA gene sequences, the isolate was found to be closely related to the genus Methylomonas in the family Methylococcaceae of the class Gammaproteobacteria with 94.2-97.4% 16S rRNA gene similarity to Methylomonas type strains...
October 2017: Journal of Microbiology / the Microbiological Society of Korea
Xi-Hui Li, Joon-Hee Lee
Biofilms are complex microbial architectures that attach to surfaces and encase microorganisms in a matrix composed of self-produced hydrated extracellular polymeric substances (EPSs). In biofilms, microorganisms become much more resistant to antimicrobial treatments, harsh environmental conditions, and host immunity. Biofilm formation by microbial pathogens greatly enhances survival in hosts and causes chronic infections that result in persistent inflammation and tissue damages. Currently, it is believed over 80% of chronic infectious diseases are mediated by biofilms, and it is known that conventional antibiotic medications are inadequate at eradicating these biofilm-mediated infections...
October 2017: Journal of Microbiology / the Microbiological Society of Korea
Hyeseon Kim, Mi-Rae Lee, Taeck Joong Jeon
FERM domain-containing proteins are involved in diverse biological and pathological processes, including cell-substrate adhesion, cell-cell adhesion, multicellular development, and cancer metastasis. In this study, we determined the functions of FrmB, a FERM domain-containing protein, in the cell morphology, cell adhesion, and multicellular development of Dictyostelium cells. Our results show that FrmB appears to play an important role in regulating the size of developmental structures. frmB null cells showed prolonged aggregation during development, resulting in increased size of developmental structures, such as mounds and fruiting bodies, compared to those of wild-type cells, whereas FrmB overexpressing cells exhibited decreased size of developmental structures...
September 2017: Journal of Microbiology / the Microbiological Society of Korea
Xueting Zou, Mengwei Weng, Xu Ji, Rong Guo, Weijiang Zheng, Wen Yao
In China, antimicrobials and copper are used extensively as growth-promoting agents for piglets. This study aimed to characterize the role of in-feed copper in the emergence of copper-tolerant and antibiotic-resistant Enterococcus and Lactobacillus isolates in Chinese pig farms. Feces of the same eight piglets from four litters at 7 and 55 days old and their mothers were traced in order to isolate Enterococcus spp. and Lactobacillus spp.. The minimum inhibitory concentrations of 10 antimicrobials and copper sulfate were determined using an agar dilution method...
September 2017: Journal of Microbiology / the Microbiological Society of Korea
Siriporn Chaikaew, Sasitorn Baipong, Teruo Sone, Apinun Kanpiengjai, Naradorn Chui-Chai, Kozo Asano, Chartchai Khanongnuch
The microbiota of lactic acid bacteria (LAB) in thirty-five samples of Miang, a traditional fermented tea leaf product, collected from twenty-two different regions of eight provinces in upper northern Thailand was revealed through the culture-dependent technique. A total of 311 presumptive LAB strains were isolated and subjected to clustering analysis based on repetitive genomic element-PCR (rep-PCR) fingerprinting profiles. The majority of the strains belonged to the Lactobacillus genera with an overwhelming predominance of the Lb...
September 2017: Journal of Microbiology / the Microbiological Society of Korea
Nabil Elderiny, Leonid N Ten, Jae-Jin Lee, Seung-Yeol Lee, Sangkyu Park, Young-Je Cho, Myung Kyum Kim, Hee-Young Jung
A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterium, designated 15J9-6(T), was isolated from beach soil on Jeju Island, South Korea. Strain 15J9-6(T), grew at 10-30°C (optimum growth at 25°C) and pH 7-8 (optimum growth at pH 7) on R2A, NA, and TSA agar. Phylogenetically, the strain was closely related to members of the genus Spirosoma (92.3-90.1% 16S rRNA gene sequence similarities) and showed highest sequence similarity to Spirosoma panaciterrae DSM 21099(T) (92.3%). The G+C content of the genomic DNA of strain 15J9-6(T) was 45...
September 2017: Journal of Microbiology / the Microbiological Society of Korea
Jaqueline López-Ochoa, J Fernando Montes-García, Candelario Vázquez, Patricia Sánchez-Alonso, Victor M Pérez-Márquez, Patrick J Blackall, Sergio Vaca, Erasmo Negrete-Abascal
Gallibacterium, which is a bacterial pathogen in chickens, can form biofilms. Amyloid proteins present in biofilms bind Congo red dye. The aim of this study was to characterize the cell-surface amyloid-like protein expressed in biofilms formed by Gallibacterium strains and determine the relationship between this protein and curli, which is an amyloid protein that is commonly expressed by members of the Enterobacteriaceae family. The presence of amyloid-like proteins in outer membrane protein samples from three strains of G...
September 2017: Journal of Microbiology / the Microbiological Society of Korea
Diana Catalina Pedraza-Zapata, Andrea Melissa Sánchez-Garibello, Balkys Quevedo-Hidalgo, Nubia Moreno-Sarmiento, Ivonne Gutiérrez-Rojas
The objective of this study was to evaluate the potential of eight fungal isolates obtained from soils in rice crops for straw degradation in situ. From the initial eight isolates, Pleurotus ostreatus T1.1 and Penicillium sp. HC1 were selected for further characterization based on qualitative cellulolytic enzyme production and capacity to use rice straw as a sole carbon source. Subsequently, cellulolytic, xylanolytic, and lignolytic (Pleurotus ostreatus) activity on carboxymethyl cellulose, oat xylan, and rice straw with different nitrogen sources was evaluated...
September 2017: Journal of Microbiology / the Microbiological Society of Korea
Can Du, Zengchao Geng, Qiang Wang, Tongtong Zhang, Wenxiang He, Lin Hou, Yueling Wang
Microbial communities in subsurface soil are specialized for their environment, which is distinct from that of the surface communities. However, little is known about the microbial communities (bacteria and fungi) that exist in the deeper soil horizons. Vertical changes in microbial alpha-diversity (Chao1 and Shannon indices) and community composition were investigated at four soil depths (0-10, 10-20, 20-40, and 40-60 cm) in a natural secondary forest of Betula albosinensis by high-throughput sequencing of the 16S and internal transcribed spacer rDNA regions...
September 2017: Journal of Microbiology / the Microbiological Society of Korea
Feng Zhang, Weijiang Zheng, Rong Guo, Wen Yao
In China's swine industry, copper is generally supplemented above the National Research Council (NRC) requirement (2012) because of its antimicrobial properties and the potential for growth promotion. Yet few are concerned about whether this excess supplementation is necessary. In this study, the 16S rRNA pyrosequencing was designed and used to investigate the effect of dietary copper level on the diversity of the fecal microbial community and the correlation of copper level with the serum level of inflammatory cytokines in Sprague-Dawley rat models...
September 2017: Journal of Microbiology / the Microbiological Society of Korea
Hyosun Lee, Dong-Uk Kim, Suyeon Lee, Sooyeon Park, Jung-Hoon Yoon, So Yoon Park, Jong-Ok Ka
A Gram-stain-negative and orangish yellow-pigmented bacterial strain, designated PR1014K(T), was isolated from an automobile evaporator core collected in Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PR1014K(T) was related with the members of the genus Spirosoma (94.7-90.2%) and closely related with Spirosoma lacussanchae CPCC 100624(T) (94.7%), Spirosoma knui 15J8-12(T) (94.3%), and Spirosoma soli MIMBbqt12(T) (93.3%). The strain grew at 15-40°C (optimum, 25°C), pH 6...
September 2017: Journal of Microbiology / the Microbiological Society of Korea
Praveen Rishi, Reena Thakur, Ujjwal Jit Kaur, Harjit Singh, Kuldip K Bhasin
One of the reasons for increased antibiotic resistance in Salmonella enterica serovar Typhi Ty2 is the influx of heavy metal ions in the sewage, from where the infection is transmitted. Therefore, curbing these selective agents could be one of the strategies to manage the emergence of multidrug resistance in the pathogen. As observed in our earlier study, the present study also confirmed the links between cadmium accumulation and antibiotic resistance in Salmonella. Therefore, the potential of a chemically-synthesised compound 2, 2'-dipyridyl diselane (DPDS) was explored to combat the metal-induced antibiotic resistance...
September 2017: Journal of Microbiology / the Microbiological Society of Korea
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"