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Journal of Microbiology / the Microbiological Society of Korea

So Yeon Kim, Sang Yop Shin, Ji-Young Rhee, Kwan Soo Ko
Carbapenem-resistant Gram-negative bacteria (CR-GNB) have emerged and disseminated worldwide, become a great concern worldwide including Korea. The prevalence of fecal carriage of imipenem-resistant Gram-negative bacteria (IR-GNB) in persons in Korea was investigated. Stool samples were collected from 300 persons upon medical examination. Samples were screened for IR-GNB by using MacConkey agar with 2 μl/ml imipenem. Species were identified by 16S rRNA gene sequence analysis, and antimicrobial susceptibility was determined by the broth microdilution method...
July 18, 2017: Journal of Microbiology / the Microbiological Society of Korea
Rui Xing, Qing-Bo Gao, Fa-Qi Zhang, Peng-Cheng Fu, Jiu-Li Wang, Hui-Ying Yan, Shi-Long Chen
Floccularia luteovirens, as an ectomycorrhizal fungus, is widely distributed in the Qinghai-Tibet Plateau. As an edible fungus, it is famous for its unique flavor. Former studies mainly focus on the chemical composition and genetic structure of this species. However, the phylogenetic relationship between genotypes remains unknown. In this study, the genetic variation and phylogenetic relationship between the genotypes of F. luteovirens in Qinghai-Tibet Plateau was estimated through the analysis on two protein-coding genes (rpb1 and ef-1α) from 398 individuals collected from 24 wild populations...
July 4, 2017: Journal of Microbiology / the Microbiological Society of Korea
Tuan Manh Nguyen, Jaisoo Kim
There are marked differences between wet and freeze-dried cells with regard to the identification of polar lipid components. The determination of the polar lipid composition of freeze-dried cells is well established. However, several approaches to identifying polar lipid components in wet cells have met with limited success owing to the presence of non-polar compounds in the extracts, resulting in a lipid composition with a narrow scope. In this study, we surveyed the lipid profiles of the wet biomasses of three Gram-positive (Microbacterium lacticum, Rhodococcus koreensis, and Streptomyces longwoodensis) and two Gram-negative (Pseudomonas aeruginosa and Novosphingobium capsulatum) bacteria; the results were comparable in quality to those obtained using a standard freeze-dried approach...
July 4, 2017: Journal of Microbiology / the Microbiological Society of Korea
Xiaoling Wang, Shuo Meng, Jingshi Han
In this study, we explored Bacillus subtilis biofilm growth under various conditions such as the use of substrates with different stiffnesses and nutrient levels using a well-developed optical imaging technique to spatially and temporally track biofilm growth. We also developed a quantitative method to characterize B. subtilis biofilm morphologies under various growth conditions. To determine biofilm rim irregularities, we used the dimensionless P2A ratio, defined as P(2)/4πA, where P is the perimeter and A is the area of the biofilm...
July 4, 2017: Journal of Microbiology / the Microbiological Society of Korea
Giovanna Rappocciolo, Frank Jenkins, Charles R Rinaldo
No abstract text is available yet for this article.
July 2017: Journal of Microbiology / the Microbiological Society of Korea
Jun Hyeong Jang, Dongwook Lee, Seho Cha, Taegun Seo
Strain Mol12(T), which presented in the form of Gram-negative, motile, non-spore forming rod-shaped, was isolated from soil in South Korea and characterized to determine its taxonomic position. The strain grew at 20-30°C (optimum 30°C) and pH 7.0-10.0 (optimum pH 8.0) with 1% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain Mol12(T) was most closely related to Ensifer terangae LMG 7834(T) (96.78%), Rhizobium daejeonense KCTC 12121(T) (96.43%), Ensifer adhaerens Casida A(T) (96...
July 2017: Journal of Microbiology / the Microbiological Society of Korea
Bangzheng Wang, Tao Niu, Muhammad Zeeshan Bhatti, Fenfen Chen, Lin Wu, Jiwu Chen
Euplotes encysticus is a species of Hypotrich ciliates, which form cyst wall by secreting the special substances on encounter of adverse environment. It has critical significance to study the component and mechanism underlying resting cyst, during resisting unfavorable conditions in dormancy induction. The present study was aimed to investigate the effects of cyst wall proteins of Euplotes encysticus by using biochemical methods. Therefore, protein extracts were separated by SDSPAGE, identified and analyzed by MALDI-TOF MS and Bioinformatics tools...
July 2017: Journal of Microbiology / the Microbiological Society of Korea
Luyao Wang, Ning Wang, Dandan Mi, Yuming Luo, Jianhua Guo
In this study, we investigate the relationship between γ-PGA productivity and biocontrol capacity of Bacillus subtilis BsE1; one bacterial isolate displayed 62.14% biocontrol efficacy against Fusarium root rot. The γ-PGA yield assay, motility assay, wheat root colonization assay, and biological control assay were analysed in different γ-PGA yield mutants of BsE1. The pgsB (PGA-synthase-CapB gene) deleted mutant of BsE1 reduced γ-PGA yield and exhibited apparent decline of in vitro motile ability. Deletion of pgsB impaired colonizing capacity of BsE1 on wheat root in 30 days, also lowered biocontrol efficacies from 62...
July 2017: Journal of Microbiology / the Microbiological Society of Korea
Tuan Manh Nguyen, Jaisoo Kim
Three Gram-negative, strictly aerobic, chemolithoheterotrophic bacterial strains, designated UCM-30, UCM-33, and UCM-39(T), were isolated in South Korea. Based on their 16S rRNA gene sequences, the three isolated strains were found to be similar to Limnobacter thiooxidans CS-K2(T) (97.41-97.68%), Limnobacter litoralis KP1-19(T) (95.55-95.76%), and various genera belonging to the class Betaproteobacteria (90.34-93.34%). DNA-DNA hybridization showed 79.3-83.9% similarity between the genomic DNA of UCM-39(T), UCM-30, and UCM-33, while the sequence similarity between UCM-39(T) and L...
July 2017: Journal of Microbiology / the Microbiological Society of Korea
Ji Young Kang, Jeesun Chun, Young Cheol Jung, Kwang Yeop Jahng
A Gram stain-negative, yellowish green-pigmented, facultatively anaerobic, motile, curved rod-shaped bacterium designated as strain JJ016(T) was isolated from an artificial lake in South Korea, and characterized using a polyphasic approach. The 16S rRNA gene sequence of strain JJ016(T) indicated that the isolate belonged to the family Rhodocyclaceae and exhibited 95.0% identity to Uliginosibacterium gangwonense 5YN10-9(T). The major cellular fatty acids of the novel strain were summed feature 3 (C16:1 ω6c and/or C16:1 ω7c), C16:0, C14:0, and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c)...
July 2017: Journal of Microbiology / the Microbiological Society of Korea
Juan Zhang, Yanqiu Cai, Guocheng Du, Jian Chen, Miao Wang, Zhen Kang
Cutinase as a promising biocatalyst has been intensively studied and applied in processes targeted for industrial scale. In this work, the cutinase gene tfu from Thermobifida fusca was artificially synthesized according to codon usage bias of Saccharomyces cerevisiae and investigated in Saccharomyces cerevisiae. Using the α-factor signal peptide, the T. fusca cutinase was successfully overexpressed and secreted with the GAL1 expression system. To increase the cutinase level and overcome some of the drawbacks of induction, four different strong promoters (ADH1, HXT1, TEF1, and TDH3) were comparatively evaluated for cutinase production...
July 2017: Journal of Microbiology / the Microbiological Society of Korea
Qingmei Liu, Muhammad Zubair Siddiqi, Mi-Sun Kim, Sang Yong Kim, Wan-Taek Im
A Gram-negative, non-motile, aerobic, and rod-shaped bacterial strain designated as BR5-28(T) was isolated from the soil of a ginseng field at Baekdu Mountain Korea, and its taxonomic position was investigated using a polyphasic approach. Strain BR5-28(T) grew at 10-42°C (optimum temperature, 30°C) and pH 5.5-8.5 (optimum pH, 7.0) on R2A agar medium without additional NaCl supplementation. Strain BR5- 28T exhibited β-glucosidase activity, which was responsible for its ability to transform the ginsenosides Rb1 and Rd (the two dominant active components of ginseng) to compound-K...
July 2017: Journal of Microbiology / the Microbiological Society of Korea
Thao Thi Nguyen, Tae-Soo Chon, Jaehan Kim, Young-Su Seo, Muyoung Heo
Secreted proteins (secretomes) play crucial roles during bacterial pathogenesis in both plant and human hosts. The identification and characterization of secretomes in the two plant pathogens Burkholderia glumae BGR1 and B. gladioli BSR3, which cause diseases in rice such as seedling blight, panicle blight, and grain rot, are important steps to not only understand the disease-causing mechanisms but also find remedies for the diseases. Here, we identified two datasets of secretomes in B. glumae BGR1 and B. gladioli BSR3, which consist of 118 and 111 proteins, respectively, using mass spectrometry approach and literature curation...
July 2017: Journal of Microbiology / the Microbiological Society of Korea
Jhung-Ahn Yang, Sung-Hyun Yang, Junghee Kim, Kae Kyoung Kwon, Hyun-Myung Oh
Here we report the comparative genomic analysis of strain UJ101 with 15 strains from the family Flavobacteriaceae, using the CGExplorer program. Flavobacteriales bacterium strain UJ101 was isolated from a xanthid crab, Atergatis reticulatus, from the East Sea near Korea. The complete genome of strain UJ101 is a 3,074,209 bp, single, circular chromosome with 30.74% GC content. While the UJ101 genome contains a number of annotated genes for many metabolic pathways, such as the Embden-Meyerhof pathway, the pentose phosphate pathway, the tricarboxylic acid (TCA) cycle, and the glyoxylate cycle, genes for the Entner-Douddoroff pathway are not found in the UJ101 genome...
July 2017: Journal of Microbiology / the Microbiological Society of Korea
Kwang-Woo Jung, Sangyong Lim, Yong-Sun Bahn
Organisms living in extreme environments have evolved a wide range of survival strategies by changing biochemical and physiological features depending on their biological niches. Interestingly, organisms exhibiting high radiation resistance have been discovered in the three domains of life (Bacteria, Archaea, and Eukarya), even though a naturally radiationintensive environment has not been found. To counteract the deleterious effects caused by radiation exposure, radiation- resistant organisms employ a series of defensive systems, such as changes in intracellular cation concentration, excellent DNA repair systems, and efficient enzymatic and non-enzymatic antioxidant systems...
July 2017: Journal of Microbiology / the Microbiological Society of Korea
Hao Wu, Guiguang Chen, Yaxi Bian, Wei Zeng, Bihong Sun, Zhiqun Liang
In this study, a new agar-degrading strain was isolated from soil with agar as a sole carbon source and energy. Based on its morphological, physiological, biochemical characterization and 16S rDNA sequence, the strain was identified as Streptomyces lavendulae UN-8. The extracellular agarase activity reached 0.03 U/ml after fermentation in shake flask (250 ml), which was close to other reported non-marine microorganisms. Furthermore, it is interesting that the growth of UN-8 would be inhibited by glucose (40 g/L) and maltose (40 g/L) with the inhibitory rate of 100% and 70%, respectively...
June 2017: Journal of Microbiology / the Microbiological Society of Korea
Yun Suk Lee, Hyun Jung Kim, Woojun Park
Although microbially induced calcium carbonate precipitation (MICP) through ureolysis has been widely studied in environmental engineering fields, urea utilization might cause environmental problems as a result of ammonia and nitrate production. In this study, many non-ureolytic calcium carbonate-precipitating bacteria that induced an alkaline environment were isolated from the rhizosphere of Miscanthus sacchariflorus near an artificial stream and their ability to precipitate calcium carbonate minerals with the absence of urea was investigated...
June 2017: Journal of Microbiology / the Microbiological Society of Korea
Byeong-Hoon Lee, Kiramage Chathuranga, Md Bashir Uddin, Prasanna Weeratunga, Myun Soo Kim, Won-Kyung Cho, Hong Ik Kim, Jin Yeul Ma, Jong-Soo Lee
Coptidis Rhizoma is derived from the dried rhizome of Ranunculaceous plants and is a commonly used traditional Chinese medicine. Although Coptidis Rhizoma is commonly used for its many therapeutic effects, antiviral activity against respiratory syncytial virus (RSV) has not been reported in detail. In this study, we evaluated the antiviral activities of Coptidis Rhizoma extract (CRE) against RSV in human respiratory tract cell line (HEp2) and BALB/c mice. An effective dose of CRE significantly reduces the replication of RSV in HEp2 cells and reduces the RSV-induced cell death...
June 2017: Journal of Microbiology / the Microbiological Society of Korea
Priyanka Singh, Yeon Ju Kim, Hina Singh, Mohamed El-Agamy Farh, Deok-Chun Yang
A novel strain DCY105(T) was isolated from soil collected from the rhizosphere of ginseng (Panax ginseng), in Gochang, Republic of Korea. Strain DCY105(T) is Gram-reaction-negative, white, non-motile, non-flagellate, rod-shaped and aerobic. The bacteria grow optimally at 30°C, pH 6.5-7.0 and in the absence of NaCl. Phylogenetically, strain DCY105(T) is most closely related to Achromobacter marplatensis LMG 26219(T) (96.81%). The DNA G+C content of strain DCY105(T) was 64.4 mol%. Ubiquinone 8 was the major respiratory quinone, and phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol were amongst the major polar lipids...
June 2017: Journal of Microbiology / the Microbiological Society of Korea
Jung-Hoon Kim, Yoon-Mo Yang, Chang-Jun Ji, Su-Hyun Ryu, Young-Bin Won, Shin-Yeong Ju, Yumi Kwon, Yeh-Eun Lee, Hwan Youn, Jin-Won Lee
PerR, a member of Fur family protein, is a metal-dependent H2O2 sensing transcription factor that regulates genes involved in peroxide stress response. Industrially important bacterium Bacillus licheniformis contains three PerR-like proteins (PerRBL, PerR2, and PerR3) compared to its close relative Bacillus subtilis. Interestingly, unlike other bacteria including B. subtilis, no authentic perR BL null mutant could be established for B. licheniformis. Thus, we constructed a conditional perR BL mutant using a xylose-inducible promoter, and investigated the genes under the control of PerRBL...
June 2017: Journal of Microbiology / the Microbiological Society of Korea
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