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Journal of Microbiology / the Microbiological Society of Korea

Jae-Bong Lee, Seon Hwa Jeon, Seok-Gwan Choi, Hee-Young Jung, Myung Kyum Kim, Sathiyaraj Srinivasan
In this paper, a new bacterial strain designated as 16MFT21(T) is isolated from the muscle of a fish caught in the Antarctic Ocean. Strain 16MFT21(T) is a Gram-staining-positive, catalase-oxidase-positive, rod-shaped facultative-aerobic bacterium. The phylogenetic analysis that is based on the 16S-rRNA gene sequence of strain 16MFT21(T) revealed that it belongs to the genus Bacillus in the family Bacillaceae in the class Bacilli. The highest degrees of the sequence similarity of the strain 16MFT21(T) is with Bacillus licheniformis ATCC 14580(T) (96...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
Hyeonheui Ham, Sosoo Kim, Min-Hee Kim, Soohyung Lee, Sung Kee Hong, Jae-Gee Ryu, Theresa Lee
To investigate contamination of ground red pepper with fungi and mycotoxin, we obtained 30 ground red pepper samples from 15 manufacturers in the main chili-pepper-producing areas in Korea. Fungal contamination was evaluated by spreading diluted samples on potato dextrose agar plates. The total fungi counts ranged from 0 to 7.3 × 10(3) CFU/g. In the samples, the genus Aspergillus had the highest incidence, while Paecilomyces was isolated most frequently. The next most frequent genera were Rhizopus, Penicillium, Cladosporium, and Alternaria...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
Jae-Jin Lee, Yeon-Hee Lee, Su-Jin Park, Sangyong Lim, Sun-Wook Jeong, Seung-Yeol Lee, Sangkyu Park, Hyo-Won Choi, Myung Kyum Kim, Hee-Young Jung
A novel Gram-positive, oval-shaped, non-motile bacterium designated strain 16F1L(T) was isolated from sediment collected from the Han River in Seoul, Republic of Korea. Based on the 16S rRNA gene sequence (1,448 bp), this strain was identified as a member of the genus Deinococcus that belongs to the class Deinococci. Similarities in the 16S rRNA gene sequence were shown with Deinococcus daejeonensis MJ27(T) (99.0%), D. grandis DSM 3963(T) (98.1%), D. radiotolerans C1(T) (97.5%), and D. caeni Ho-08(T) (97.2%)...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
Seok-Gwan Choi, Seon Hwa Jeon, Jae-Bong Lee, Eun Sun Joo, Sangyong Lim, Hee-Young Jung, Myung Kyum Kim
Two new bacterial strains designated as Ant6(T) and Ant18 were isolated from the muscle of a fish which had been caught in the Antarctic Ocean. Both strains are Gram-stain-positive, catalase positive, oxidase negative, aerobic, and coccoid bacteria. Phylogenetic analysis based on the 16S rRNA gene sequences of strains Ant6(T) and Ant18 revealed that the strains Ant6(T) and Ant18 belong to the genus Deinococcus in the family Deinococcaceae in the class Deinococci. The highest degrees of sequence similarities of strains Ant6(T) and Ant18 were found with Deinococcus alpinitundrae LMG 24283(T) by 96...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
Md Bashir Uddin, Byeong-Hoon Lee, Chamilani Nikapitiya, Jae-Hoon Kim, Tae-Hwan Kim, Hyun-Cheol Lee, Choul Goo Kim, Jong-Soo Lee, Chul-Joong Kim
Bee venom (BV) from honey bee (Apis Melifera L.) contains at least 18 pharmacologically active components including melittin (MLT), phospholipase A2 (PLA2), and apamin etc. BV is safe for human treatments dose dependently and proven to possess different healing properties including antibacterial and antiparasitidal properties. Nevertheless, antiviral properties of BV have not well investigated. Hence, we identified the potential antiviral properties of BV and its component against a broad panel of viruses. Co-incubation of non-cytotoxic amounts of BV and MLT, the main component of BV, significantly inhibited the replication of enveloped viruses such as Influenza A virus (PR8), Vesicular Stomatitis Virus (VSV), Respiratory Syncytial Virus (RSV), and Herpes Simplex Virus (HSV)...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
Jin-Woo Lee, Kae Kyoung Kwon, Jang-Jun Bahk, Dong-Hun Lee, Hyun Sook Lee, Sung Gyun Kang, Jung-Hyun Lee
We have previously identified a sulfate methane transition zone (SMTZ) within the methane hydrate-bearing sediment in the Ulleung Basin, East Sea of Korea, and the presence of ANME-1b group in the sediment has been shown by phylogenetic analysis of a 16S rRNA gene. Herein, we describe taxonomic and functional profiling in the SMTZ sample by metagenomic analysis, comparing with that of surface sediment. Metagenomic sequences of 115 Mbp and 252 Mbp were obtained from SMTZ and surface sediments, respectively. The taxonomic profiling using BLASTX against the SEED within MG-RAST showed the prevalence of methanogens (19...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
Shin Ae Lee, Jiyoung Park, Bora Chu, Jeong Myeong Kim, Jae-Ho Joa, Mee Kyung Sang, Jaekyeong Song, Hang-Yeon Weon
The microbiome in the rhizosphere-the region surrounding plant roots-plays a key role in plant growth and health, enhancing nutrient availability and protecting plants from biotic and abiotic stresses. To assess bacterial diversity in the tomato rhizosphere, we performed two contrasting approaches: culture-dependent and -independent. In the culture-dependent approach, two culture media (Reasoner's 2A agar and soil extract agar) were supplemented with 12 antibiotics for isolating diverse bacteria from the tomato rhizosphere by inhibiting predominant bacteria...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
Aeryun Kim, Stephanie L Servetas, Jieun Kang, Jinmoon Kim, Sungil Jang, Yun Hui Choi, Hanfu Su, Yeong-Eui Jeon, Youngmin A Hong, Yun-Jung Yoo, D Scott Merrell, Jeong-Heon Cha
The array of outer membrane proteins (OMPs) found in Helicobacter pylori provides a crucial component for persistent colonization within the gastric niche. Not only does H. pylori harbor a wide number of OMPs, but these OMPs often vary across strains; this likely contributes to immune evasion, adaptation during long term colonization, and potentially differential disease progression. Previous work from our group described OMP differences among the Bab family (babA, babB, and babC) and Hom family (homA and homB) from 80 American H...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
Kyung Sook Bae, Mi Sun Kim, Ji Hee Lee, Joo Won Kang, Dae In Kim, Ji Hee Lee, Chi Nam Seong
To understand the isolation and classification state of actinobacterial species with valid names for Korean indigenous isolates, isolation source, regional origin, and taxonomic affiliation of the isolates were studied. At the time of this writing, the phylum Actinobacteria consisted of only one class, Actinobacteria, including five subclasses, 10 orders, 56 families, and 330 genera. Moreover, new taxa of this phylum continue to be discovered. Korean actinobacterial species with a valid name has been reported from 1995 as Tsukamurella inchonensis isolated from a clinical specimen...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
Jeong Myeong Kim, An-Sung Roh, Seung-Chul Choi, Eun-Jeong Kim, Moon-Tae Choi, Byung-Koo Ahn, Sun-Kuk Kim, Young-Han Lee, Jae-Ho Joa, Seong-Soo Kang, Shin Ae Lee, Jae-Hyung Ahn, Jaekyeong Song, Hang-Yeon Weon
Soil microorganisms play an essential role in soil ecosystem processes such as organic matter decomposition, nutrient cycling, and plant nutrient availability. The land use for greenhouse cultivation has been increasing continuously, which involves an intensive input of agricultural materials to enhance productivity; however, relatively little is known about bacterial communities in greenhouse soils. To assess the effects of environmental factors on the soil bacterial diversity and community composition, a total of 187 greenhouse soil samples collected across Korea were subjected to bacterial 16S rRNA gene pyrosequencing analysis...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
Shin Ae Lee, Jeong Myeong Kim, Jae-Hyung Ahn, Jae-Ho Joa, Soo-Jin Kim, Mee-Kyung Sang, Jaekyeong Song, Soon-Wo Kwon, Hang-Yeon Weon
Strain 63MJ-2(T) was isolated from the feces of broad-winged katydid (Pseudorhynchus japonicus) collected in Korea. The 16S rRNA gene sequence of this strain showed the highest sequence similarity with that of Siphonobacter aquaeclarae P2(T) (96.1%) and had low similarities (below 86.3%) with those of other members of family 'Flexibacteraceae'. The strain 63MJ-2(T) is a strictly aerobic, Gram-stain-negative, non-motile, rod-shaped bacterium. The strain grew at 4-35°C (optimum, 25-30°C), pH of 5.0-9.0 (optimum, 6...
November 2016: Journal of Microbiology / the Microbiological Society of Korea
Qin-Yi Wu, Yue-Yan Zhu, Cheng-Gang Zou, Ying-Qian Kang, Lian-Ming Liang
Turgor is very important for the invasive growth of fungal pathogens. Glycerol, a highly osmotic solvent, is considered to play an important role in turgor generation. The nematophagous fungus Arthrobotrys oligospora mainly lives as a saprophyte. In the presence of nematodes, A. oligospora enters the parasitic stage by forming three-dimensional networks (traps) to capture nematodes. In A. oligospora, we found that glycerol accumulated during nematode-induced trap formation. We demonstrated that deleting gph1, which encodes glycogen phosphorylase, decreased the glycerol content, compared with that of a wild-type strain...
November 2016: Journal of Microbiology / the Microbiological Society of Korea
Ki Woo Kim
Microorganisms, or microbes, can function as threatening pathogens that cause disease in humans, animals, and plants; however, they also act as litter decomposers in natural ecosystems. As the outermost barrier and interface with the environment, the microbial cell surface is crucial for cell-to-cell communication and is a potential target of chemotherapeutic agents. Surface ultrastructures of microbial cells have typically been observed using scanning electron microscopy (SEM) and atomic force microscopy (AFM)...
November 2016: Journal of Microbiology / the Microbiological Society of Korea
Aliz Bozó, Marianna Domán, László Majoros, Gábor Kardos, István Varga, Renátó Kovács
Farnesol is a quorum-sensing molecule that inhibits biofilm formation in Candida albicans. Previous in vitro data suggest that, in combination with certain antifungals, farnesol may have an adjuvant anti-biofilm agent. However, the in vivo efficacy of farnesol is very questionable. Therefore, the in vitro and in vivo activity of fluconazole combined with farnesol was evaluated against C. albicans biofilms using fractional inhibitory concentration index (FICI) determination, time-kill experiments and a murine vulvovaginitis model...
November 2016: Journal of Microbiology / the Microbiological Society of Korea
Miguel David Marfil-Santana, Aileen O'Connor-Sánchez, Jorge Humberto Ramírez-Prado, Cesar De Los Santos-Briones, López-Aguiar, Korynthia Lluvia, Rafael Rojas-Herrera, Asunción Lago-Lestón, Alejandra Prieto-Davó
The need for new antibiotics has sparked a search for the microbes that might potentially produce them. Current sequencing technologies allow us to explore the biotechnological potential of microbial communities in diverse environments without the need for cultivation, benefitting natural product discovery in diverse ways. A relatively recent method to search for the possible production of novel compounds includes studying the diverse genes belonging to polyketide synthase pathways (PKS), as these complex enzymes are an important source of novel therapeutics...
November 2016: Journal of Microbiology / the Microbiological Society of Korea
Hsin-Chih Lai, Yu-Tze Horng, Pen-Fang Yeh, Jann-Yuan Wang, Chin-Chung Shu, Chia-Chen Lu, Jang-Jih Lu, Jen-Jyh Lee, Po-Chi Soo
Pulmonary tuberculosis (TB) is caused by Mycobacterium tuberculosis. The protein composition of sputum may reflect the immune status of the lung. This study aimed to evaluate the protein profiles in spontaneous sputum samples from patients with active pulmonary TB. Sputum samples were collected from patients with pulmonary TB and healthy controls. Western blotting was used to analyze the amount of interleukin 10 (IL-10), interferon-gamma (IFN-γ), IL-25, IL-17, perforin-1, urease, albumin, transferrin, lactoferrin, adenosine deaminase (also known as adenosine aminohydrolase, or ADA), ADA-2, granzyme B, granulysin, and caspase-1 in sputum...
November 2016: Journal of Microbiology / the Microbiological Society of Korea
Marta O Domingos, Keyde C M Melo, Irys Viana Neves, Cristiane M Mota, Rita C Ruiz, Bruna S Melo, Raphael C Lima, Denise S P Q Horton, Monamaris M Borges, Marcia R Franzolin
Using clonal phylogenetic methods, it has been demonstrated that O111:H25 atypical enteropathogenic E. coli (aEPEC) strains belong to distinct clones, suggesting the possibility that their ability to interact with different hosts and abiotic surfaces can vary from one clone to another. Accordingly, the ability of O111:H25 aEPEC strains derived from human, cat and dogs to adhere to epithelial cells has been investigated, along with their ability to interact with macrophages and to form biofilms on polystyrene, a polymer used to make biomedical devices...
November 2016: Journal of Microbiology / the Microbiological Society of Korea
Suhyuk Lee, Jaehyuk Jang, Hyungtaek Jeon, Jisu Lee, Seung-Min Yoo, Jinsung Park, Myung-Shin Lee
Kaposi's sarcoma-associated herpesvirus (KSHV) is the major etiologic agent of Kaposi's sarcoma, primary effusion lymphoma, and multicentric Castleman's disease. Recent studies have indicated that KSHV can be detected at high frequency in patient-derived bladder cancer tissue and might be associated with the pathogenesis of bladder cancer. Bladder cancer is the second most common cancer of the genitourinary tract, and it has a high rate of recurrence. Because drug resistance is closely related to chemotherapy failure and cancer recurrence, we investigated whether KSHV infection is associated with drug resistance of bladder cancer cells...
November 2016: Journal of Microbiology / the Microbiological Society of Korea
Lee Pei Lee Angel, Mohd Termizi Yusof, Intan Safinar Ismail, Bonnie Tay Yen Ping, Intan Nur Ainni Mohamed Azni, Norman Hj Kamarudin, Shamala Sundram
Ganoderma boninense is the causal agent of a devastating disease affecting oil palm in Southeast Asian countries. Basal stem rot (BSR) disease slowly rots the base of palms, which radically reduces productive lifespan of this lucrative crop. Previous reports have indicated the successful use of Trichoderma as biological control agent (BCA) against G. boninense and isolate T. virens 7b was selected based on its initial screening. This study attempts to decipher the mechanisms responsible for the inhibition of G...
November 2016: Journal of Microbiology / the Microbiological Society of Korea
Jae-Hyung Ahn, Shin Ae Lee, Jeong Myeong Kim, Myung-Sook Kim, Jaekyeong Song, Hang-Yeon Weon
Fertilization and the response of the soil microbial community to the process significantly affect crop yield and the environment. In this study, the seasonal variation in the bacterial communities in rice field soil subjected to different fertilization treatments for more than 50 years was investigated using 16S rRNA sequencing. The simultaneous application of inorganic fertilizers and rice straw compost (CAPK) maintained the species richness of the bacterial communities at levels higher than that in the case of non-fertilization (NF) and application of inorganic fertilizers only (APK) in the initial period of rice growth...
November 2016: Journal of Microbiology / the Microbiological Society of Korea
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