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Journal of Microbiology / the Microbiological Society of Korea

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https://www.readbyqxmd.com/read/30136260/cultivable-butyrate-producing-bacteria-of-elderly-japanese-diagnosed-with-alzheimer-s-disease
#1
Thi Thuy Tien Nguyen, Yuta Fujimura, Iyo Mimura, Yusuke Fujii, Ngoc Luong Nguyen, Kensuke Arakawa, Hidetoshi Morita
The group of butyrate-producing bacteria within the human gut microbiome may be associated with positive effects on memory improvement, according to previous studies on dementia- associated diseases. Here, fecal samples of four elderly Japanese diagnosed with Alzheimer's disease (AD) were used to isolate butyrate-producing bacteria. 226 isolates were randomly picked, their 16S rRNA genes were sequenced, and assigned into sixty OTUs (operational taxonomic units) based on BLASTn results. Four isolates with less than 97% homology to known sequences were considered as unique OTUs of potentially butyrate-producing bacteria...
August 22, 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/30136259/microbial-diversity-in-the-rumen-reticulum-omasum-and-abomasum-of-yak-on-a-rapid-fattening-regime-in-an-agro-pastoral-transition-zone
#2
Dan Xue, Huai Chen, Xiaolin Luo, Jiuqiang Guan, Yixin He, Xinquan Zhao
The ruminant digestive system harbors a complex gut microbiome, which is poorly understood in the case of the four stomach compartments of yak. High-throughput sequencing and quantitative PCR were used to analyse microbial communities in the rumen, reticulum, omasum, and abomasum of six domesticated yak. The diversity of prokaryotes was higher in reticulum and omasum than in rumen and abomasum. Bacteroidetes predominated in the four stomach compartments, with abundance gradually decreasing in the trend rumen > reticulum > omasum > abomasum...
August 22, 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/30136258/brevibacterium-anseongense-sp-nov-isolated-from-soil-of-ginseng-field
#3
Mi-Seon Jung, Xiao-Tian Quan, Muhammad Zubair Siddiqi, Qingzhen Liu, Sang Yong Kim, Ji-Hyang Wee, Wan Taek Im
A Gram-positive, aerobic, non-motile, pale-yellow, and rodshaped bacterium, designated as Gsoil 188T , was isolated from the soil of a ginseng field in Pocheon, South Korea. A phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to B. epidermidis NBRC 14811T (98.4%), B. sediminis FXJ8.269T (98.2%), B. avium NCFB 3055T (98.1%), and B. oceani BBH7T (98.1%), while it shared less than 98.1% identity with the other species of this genus...
August 22, 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/30136257/roles-of-eif4e-binding-protein-caf20-in-ste12-translation-and-p-body-formation-in-yeast
#4
Kiyoung Park, Yu-Seon Lee, Daehee Jung, Jinmi Kim
Translation initiation factor eIF4E forms eIF4E-eIF4G complex at the 5' cap of mRNA. This interaction can be inhibited by the family of 4E-binding proteins (4E-BP). In yeast Saccharomyces cerevisiae, two 4E-BPs, Caf20 and Eap1, compete with eIF4G for binding to eIF4E via the shared conserved interaction motif. In order to investigate the roles of Caf20 in gene-specific translational regulation and the formation of mRNA granules (P-bodies), we introduced substitution mutations, caf20-Y4A or caf20-L9A, in the eIF4E-binding motif for CAF20...
August 22, 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/30141163/erratum-to-variation-of-cassiicolin-genes-among-chinese-isolates-of-corynespora-cassiicola
#5
Jun Wu, Xuewen Xie, Yanxia Shi, Ali Chai, Qi Wang, Baoju Li
In the online published article by Wu et al. since 30 July 2018, the figure 4 is unfortunately incorrect. The figure 4 should be corrected as below.
September 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/30141162/retraction-note-interferon-mediated-antiviral-activities-of-angelica-tenuissima-nakai-and-its-active-components
#6
Prasanna Weeratunga, Md Bashir Uddin, Myun Soo Kim, Byeong-Hoon Lee, Tae-Hwan Kim, Ji-Eun Yoon, Jin Yeul Ma, Hongik Kim, Jong-Soo Lee
The above article by Weeratunga et al. has been retracted from Journal of Microbiology at the request of the corresponding author. The authors found that they were unable to reproduce Figure 1, Figure 3(A), Figure 4(A) and Figure 7(D) presented in this paper. All of the authors agreed to this retraction. The authors regret any inconvenience that this may cause and apologize sincerely to the readers, reviewers, and editors of Journal of Microbiology.
September 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/30141161/antiviral-activity-of-schizonepeta-tenuifolia-briquet-against-noroviruses-via-induction-of-antiviral-interferons
#7
Yee Ching Ng, Ye Won Kim, Jeong-Su Lee, Sung Joon Lee, Moon Jung Song
Human noroviruses are the causative agents of non-bacterial gastroenteritis worldwide. The rapid onset and resolution of disease symptoms suggest that innate immune responses are critical for controlling norovirus infection; however, no effective antivirals are yet available. The present study was conducted to examine the antiviral activities of Schizonepeta tenuifolia Briquet extract (STE) against noroviruses. Treatment of human norovirus replicon-bearing HG23 cells with STE at 5 and 10 mg/ml concentrations resulted in the reduction in the viral RNA levels by 77...
September 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/30141160/diet-induced-obese-mice-exhibit-altered-immune-responses-to-early-salmonella-typhimurium-oral-infection
#8
Ricardo Ernesto Ramírez-Orozco, Elena Franco Robles, Victoriano Pérez Vázquez, Joel Ramírez Emiliano, Marco Antonio Hernández Luna, Sergio López Briones
Obesity is a chronic disease associated with different metabolic diseases as well as alterations in immune cell function. It is characterized by a chronic systemic low grade inflammation. There are several studies demonstrating the influence of obesity on the impaired immune response to infection. However, it is not completely clear whether the obese environment influences the development or maintenance of the immune response against infections. The aim of this study was to determine how obesity induced by a high-fat diet affects the immune response to an early oral Salmonella infection...
September 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/30141159/comparison-of-virulence-between-matt-and-mucoid-colonies-of-klebsiella-pneumoniae-coproducing-ndm-1-and-oxa-232-isolated-from-a-single-patient
#9
Haejeong Lee, Jin Yang Baek, So Yeon Kim, HyunJi Jo, KyeongJin Kang, Jae-Hoon Ko, Sun Young Cho, Doo Ryeon Chung, Kyong Ran Peck, Jae-Hoon Song, Kwan Soo Ko
Nine Klebsiella pneumoniae isolates coproducing NDM-1 and OXA-232 carbapenemases were successively isolated from a single patient. Although they were isolated simultaneously and were isogenic, they presented different colony phenotypes (matt and mucoid). All nine isolates were resistant to most antibiotics except colistin and fosfomycin. In addition, matt-type isolates were resistant to tigecycline. No differences were detected in the cps cluster sequences, except for the insertion of IS5 in the wzb gene of two matt-type isolates...
September 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/30141158/overexpression-and-characterization-of-a-novel-cold-adapted-and-salt-tolerant-gh1-%C3%AE-glucosidase-from-the-marine-bacterium-alteromonas-sp-l82
#10
Jingjing Sun, Wei Wang, Congyu Yao, Fangqun Dai, Xiangjie Zhu, Junzhong Liu, Jianhua Hao
A novel gene (bgl) encoding a cold-adapted β-glucosidase was cloned from the marine bacterium Alteromonas sp. L82. Based on sequence analysis and its putative catalytic conserved region, Bgl belonged to the glycoside hydrolase family 1. Bgl was overexpressed in E. coli and purified by Ni2+ affinity chromatography. The purified recombinant β-glucosidase showed maximum activity at temperatures between 25°C to 45°C and over the pH range 6 to 8. The enzyme lost activity quickly after incubation at 40°C. Therefore, recombinant β-glucosidase appears to be a cold-adapted enzyme...
September 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/30141157/fluorescence-change-of-fusobacterium-nucleatum-due-to-porphyromonas-gingivalis
#11
Min-Ah Lee, Si-Mook Kang, Se-Yeon Kim, Ji-Soo Kim, Jin-Bom Kim, Seung-Hwa Jeong
The aim of this study was to measure changes in the fluorescence of Fusobacterium nucleatum interacting with Porphyromonas gingivalis for excitation with blue light at 405-nm. P. gingivalis was mono- and co-cultivated in close proximity with F. nucleatum. The fluorescence of the bacterial colonies was photographed using a QLF-D (Quantitative Light-induced Fluorescence-Digital) Biluminator camera system with a 405 nm light source and a specific filter. The red, green and blue intensities of fluorescence images were analyzed using the image analysis software...
September 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/30141156/bacteroides-sedimenti-sp-nov-isolated-from-a-chloroethenes-dechlorinating-consortium-enriched-from-river-sediment
#12
Mohamed Ismaeil, Naoko Yoshida, Arata Katayama
A Gram-negative, anaerobic, non-motile, non-spore-forming bacterial strain, designated YN3PY1T , was isolated from a chloroethene-dechlorinating consortium originally enriched from river sediment. The strain enhanced the dechlorination of cis-dichloroethene to ethene by Dehalococcoides, especially at the early stages of cultivation. Strain YN3PY1T was the first isolate of the genus Bacteroides, obtained from animal-independent environments, and its 16S rRNA gene had the highest sequence similarity (97.1%) with Bacteroides luti JCM 19020T in the 'Coprosuis' clade of the genus Bacteroides...
September 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/30141155/aestuariibaculum-marinum-sp-nov-a-marine-bacterium-isolated-from-seawater-in-south-korea
#13
Jiwon Choi, Dongwook Lee, Jun Hyeong Jang, Seho Cha, Taegun Seo
A Gram-negative, non-motile, aerobic bacterium, designated strain IP7T , was isolated from seawater at the shore of the Incheon Eulwang-ri beach, South Korea. Cells of strain IP7T are straight or slightly rod-shaped and colonies are round, convex and orange-yellow. Strain IP7T is flexirubin-negative, mild halophile, catalase-and oxidase-positive, and produces a yellow-orange carotenoid pigment. Growth is optimal at 30°C, pH 7-9, and 2.0-4.0% NaCl (w/v). On the basis of 16S rRNA gene sequence similarity, strain IP7T is affiliated with genus Aestuariibaculum in the family Flavobacteriaceae, the closest relative being Aestuariibaculum suncheonense SC17T (98...
September 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/30141154/new-perspectives-of-lactobacillus-plantarum-as-a-probiotic-the-gut-heart-brain-axis
#14
REVIEW
Yen-Wenn Liu, Min-Tze Liong, Ying-Chieh Tsai
Lactobacillus plantarum is a non-gas-producing lactic acid bacterium that is generally regarded as safe (GRAS) with Qualified Presumption of Safety (QPS) status. Although traditionally used for dairy, meat and vegetable fermentation, L. plantarum is gaining increasing significance as a probiotic. With the newly acclaimed gut-heart-brain axis, strains of L. plantarum have proven to be a valuable species for the development of probiotics, with various beneficial effects on gut health, metabolic disorders and brain health...
September 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/30054816/suca-dependent-uptake-of-sucrose-across-the-outer-membrane-of-caulobacter-crescentus
#15
Samantha K Modrak, Martha E Melin, Lisa M Bowers
Caulobacter crescentus is an aquatic Gram-negative bacterium that lives in nutrient-poor environments. Like several other aquatic and phytopathogenic bacteria, Caulobacter cells have a relatively large number of genes predicted to encode TonB-dependent receptors (TBDRs). TBDRs transport nutrients across the outer membrane using energy from the proton motive force. We identified one TBDR gene, sucA, which is situated within a cluster of genes predicted to encode a lacI-family transcription factor (sucR), amylosucrase (sucB), fructokinase (sucC), and an inner membrane transporter (sucD)...
September 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/30054815/variation-of-cassiicolin-genes-among-chinese-isolates-of-corynespora-cassiicola
#16
Jun Wu, Xuewen Xie, Yanxia Shi, Ali Chai, Qi Wang, Baoju Li
Corynespora cassiicola is a species of fungus that is a plant pathogen of many agricultural crop plants, including severe target spot disease on cucumber. Cassiicolin is an important effector of pathogenicity of this fungus. In this study, we collected 141 Corynespora isolates from eighteen hosts, and the casscolin gene was detected in 82 C. cassiicola strains. The deduced protein sequences revealed that 72 isolates contained the Cas2 gene, two strains from Gynura bicolor harboured the Cas2.2 gene, and 59 isolates without a cassiicolin gene were classified as Cas0...
September 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/30047089/development-and-validation-of-multiplex-real-time-pcr-assays-for-rapid-detection-of-cytomegalovirus-epstein-barr-virus-and-polyomavirus-bk-in-whole-blood-from-transplant-candidates
#17
Kyung-Ah Hwang, Ji Hoon Ahn, Jae-Hwan Nam
Transplant recipients are more susceptible to bacterial and viral infections. Cytomegalovirus (CMV), Epstein-Barr virus (EBV), and polyomavirus BK (BK) are risk factors for graft dysfunction. All three of them are latent viruses that can cause serious disease in immunocompromised patients. Mainly qualitative PCR tests are required for diagnosis and quantitative monitoring, which are used to follow the response to transplantation. We developed a multiplex real-time PCR (qPCR) method to detect these viruses during blood screenings of transplant recipients...
August 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/30047088/antiviral-activity-of-poncirus-trifoliata-seed-extract-against-oseltamivir-resistant-influenza-virus
#18
Yoonki Heo, Yeondong Cho, Kwon Sung Ju, Hansam Cho, Ki Hoon Park, Hanul Choi, Jong Kwang Yoon, Chiung Moon, Young Bong Kim
The emergence of oseltamivir-resistant variants of influenza virus has highlighted the necessity for the development of more effective novel antiviral drugs. To date, numerous researchers have focused on developing antiviral drugs using natural resources, such as traditional herbal medicines. Poncirus trifoliata is widely used in oriental medicine as a remedy for gastritis, dysentery, inflammation and digestive ulcers. In this study, we investigated the potential antiviral effect of the Poncirus trifoliata orange seed extract against influenza virus...
August 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/30047087/gamma-irradiation-of-streptococcus-pneumoniae-for-the-use-as-an-immunogenic-whole-cell-vaccine
#19
Min Yong Jwa, Soyoung Jeong, Eun Byeol Ko, A Reum Kim, Hyun Young Kim, Sun Kyung Kim, Ho Seong Seo, Cheol-Heui Yun, Seung Hyun Han
Streptococcus pneumoniae is a major respiratory pathogen that causes millions of deaths worldwide. Although subunit vaccines formulated with the capsular polysaccharides or their protein conjugates are currently-available, low-cost vaccines with wide serotype coverage still remain to be developed, especially for developing countries. Recently, gamma- irradiation has been considered as an effective inactivation method to prepare S. pneumoniae vaccine candidate. In this study, we investigated the immunogenicity and protective immunity of gamma-irradiated S...
August 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/30047086/biosynthesis-of-2-amino-3-hydroxycyclopent-2-enone-moiety-of-bafilomycin-in-kitasatospora-cheerisanensis-kctc2395
#20
Nguyen Phan Kieu Hanh, Jae Yoon Hwang, Hye Ryeung Oh, Geum Jin Kim, Hyukjae Choi, Doo Hyun Nam
Bafilomycins produced by Kitasatospora cheerisanensis KCTC- 2395 belong to the 16-membered macrolactone family plecomacrolide antibiotics. Bafilomycin B1 contains 2-amino- 3-hydroxycyclopent-2-enone (C5 N), a five membered ring, which gets condensed via an amide linkage to bafilomycin polyketide. To study the biosynthetic pathway of C5 N during bafilomycin biosynthesis in K. cheerisanensis KCTC2395, we attempted the functional analysis of two putative genes, encoding 5-aminolevulinic acid synthase (ALAS) and acyl- CoA ligase (ACL)...
August 2018: Journal of Microbiology / the Microbiological Society of Korea
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