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Journal of Microbiology / the Microbiological Society of Korea

Ademir Sergio Ferreira de Araujo, Walderly Melgaço Bezerra, Vilma Maria Dos Santos, Luis Alfredo Pinheiro Leal Nunes, Maria do Carmo Catanho Pereira de Lyra, Marcia do Vale Barreto Figueiredo, Vania Maria Maciel Melo
The preserved Cerrado from Northeastern Brazil presents different physicochemical properties and plant diversity, which can influence the fungal communities. Therefore, we evaluated the fungal diversity in preserved sites, at Sete Cidades National Park, across a gradient of vegetation that included Campo graminoide, Cerrado stricto sensu, Cerradao, and Floresta decidual. Of all of the operational taxonomic units (OTUs) obtained, the Floresta decidual presented the highest richness. Ascomycota were the most abundant phylum (45%), followed by Basidiomycota (32%)...
January 27, 2017: Journal of Microbiology / the Microbiological Society of Korea
Tuan Manh Nguyen, Jaisoo Kim
White and pale yellow coloured bacteria were isolated from the riverside soil, Daejeon, South Korea, and were designated UCM-11(T), UCM-F25, and UCM-80(T). We found that all strains were able to reduce nitrate, and the cells were aerobic and motile. The DNA G+C contents of UCM-11(T), UCM-F25, and UCM-80(T) were between 68.9 to 71.2 mol% and the main ubiquinone was observed as Q-8. Based on16S rRNA gene sequences, strains UCM-11(T) and UCM-F25 were found to closely match with Azohydromonas australica IAM 12664(T) (98...
January 26, 2017: Journal of Microbiology / the Microbiological Society of Korea
Leonid N Ten, Yeon-Hee Lee, Jae-Jin Lee, Su-Jin Park, Seung-Yeol Lee, Sangkyu Park, DaeSung Lee, In-Kyu Kang, Hee-Young Jung
A Gram-stain-negative, non-motile, non-spore-forming, rodshaped, aerobic bacterial strain, designated 16F3Y-2(T), was isolated from the Han River, South Korea, and was characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 16F3Y-2(T) belonged to the family Cytophagaceae in the phylum Bacteroidetes and was most closely related to 'Hymenobacter terrae' DG7A (98.01%), H. soli PB17(T) (97.26%), H. glaciei VUG-A130(T) (96.78%), H. antarcticus VUG-A42aa(T) (96...
January 26, 2017: Journal of Microbiology / the Microbiological Society of Korea
Shuang Wang, Jingmin Gu, Meng Lv, Zhimin Guo, Guangmou Yan, Ling Yu, Chongtao Du, Xin Feng, Wenyu Han, Changjiang Sun, Liancheng Lei
Bacteriophage endolysin is one of the most promising antibiotic substitutes, but in Gram-negative bacteria, the outer membrane prevents the lysin from hydrolyzing peptidoglycans and blocks the development of lysin applications. The prime strategy for new antibiotic substitutes is allowing lysin to access the peptidoglycan from outside of the bacteria by reformation of the lysin. In this study, the novel Escherichia coli (E. coli) phage lyase lysep3, which lacks outside-in catalytic ability, was fused with the N-terminal region of the Bacillus amyloliquefaciens lysin including its cell wall binding domain D8 through the best manner of protein fusion based on the predicted tertiary structure of lysep3-D8 to obtain an engineered lysin that can lyse bacteria from the outside...
January 26, 2017: Journal of Microbiology / the Microbiological Society of Korea
Jin-Soo Park, Inseong Joe, Paul Daong Rhee, Choon-Soo Jeong, Gajin Jeong
Some species of lactic acid bacteria have been shown to be beneficial in inflammatory bowel disease (IBD). In the present study, a strain of lactic acid bacterium (Lactobacillus paracasei LS2) was isolated from the Korean food, kimchi, and was shown to inhibit the development of experimental colitis induced by dextran sulfate sodium (DSS). To investigate the role of LS2 in IBD, mice were fed DSS in drinking water for seven days along with LS2 bacteria which were administered intragastrically to some of the mice, while phosphate- buffered saline (PBS) was administered to others (the controls)...
January 26, 2017: Journal of Microbiology / the Microbiological Society of Korea
Si Won Kim, Seong Won Nho, Se Pyeong Im, Jung Seok Lee, Jae Wook Jung, Jassy Mary S Lazarte, Jaesung Kim, Woo-Jai Lee, Jeong-Ho Lee, Tae Sung Jung
Streptococcus iniae causes severe mortalities among cultured marine species, especially in the olive flounder (Paralichthys olivaceus), which is economically important in Korea and Japan. Recently, there has been growing concern regarding the emergence of S. iniae as a zoonotic pathogen. Here, 89 S. iniae isolates obtained from diseased olive flounders collected from 2003 to 2008 in Jeju Island, South Korea, were characterized using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)...
January 26, 2017: Journal of Microbiology / the Microbiological Society of Korea
Hyeseon Kim, Dong-Yeop Shin, Taeck Joong Jeon
Establishment of cell polarity is mediated by a series of signaling molecules that are asymmetrically activated or localized in the cell upon extracellular stimulation. To understand the mechanism that mediates anterior/posterior asymmetric localization of RapGAP3 during migration, we determined the minimally required amino acids in the I/LWEQ domain that cause posterior localization and found that the minimal region of the F-actin binding domain for posterior localization could, with some additional deletion at the C-terminal, localize to the anterior...
January 26, 2017: Journal of Microbiology / the Microbiological Society of Korea
YingWu Shi, Chun Li, HongMei Yang, Tao Zhang, Yan Gao, Min Chu, Jun Zeng, Qing Lin, OuTiKuEr, YuGuo Li, Xiangdong Huo, Kai Lou
This study details the introduction of a gfp marker into an endophytic bacterial strain (Achromobacter marplatensis strain 17, isolated from sugar beet) to monitor its colonization of sugar beet (Beta. vulgaris L.). Stability of the plasmid encoding the gfp was confirmed in vitro for at least 72 h of bacterial growth and after the colonization of tissues, under nonselective conditions. The colonization was observed using fluorescence microscopy and enumeration of culturable endophytes in inoculated sugar beet plants that grew for 10 or 20 days...
January 26, 2017: Journal of Microbiology / the Microbiological Society of Korea
Rina Hidayati Pratiwi, Iman Hidayat, Muhammad Hanafi, Wibowo Mangunwardoyo
This study's aim was to determine the identity of antibacterial compounds produced by Pseudomonas aeruginosa strain UICC B-40 and describe the antibacterial compounds' mechanisms of action for damaging pathogenic bacteria cells. Isolation and identification of the compounds were carried out using thin layer chromatography (TLC), nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography mass spectrometry (LC-MS) analyses. Antibacterial activity was assayed via minimum inhibitory concentration (MIC) and the antibacterial compound mechanism was observed morphologically through scanning electron microscopy (SEM)...
January 26, 2017: Journal of Microbiology / the Microbiological Society of Korea
Myung Soo Park, Seobihn Lee, Young Woon Lim
Agarum clathratum, brown algae, play important ecological roles in marine ecosystem, but can cause secondary environment pollution when they pile up on the beach. In order to resolve the environment problem by A. clathratum, we focus to isolate and identify Penicillium because many species are well known to produce extracellular enzymes. A total of 32 Penicillium strains were isolated from A. clathratum samples that collected from 13 sites along the mid-east coast of Korea in summer. They were identified based on morphological characters and phylogenetic analysis using β-tubulin DNA sequences as well as a combined dataset of β-tubulin and calmodulin...
January 26, 2017: Journal of Microbiology / the Microbiological Society of Korea
Elena Vetchinkina, Maria Kupryashina, Vladimir Gorshkov, Marina Ageeva, Yuri Gogolev, Valentina Nikitina
The morphogenesis of macromycetes is a complex multilevel process resulting in a set of molecular-genetic, physiologicalbiochemical, and morphological-ultrastructural changes in the cells. When the xylotrophic basidiomycetes Lentinus edodes, Grifola frondosa, and Ganoderma lucidum were grown on wood waste as the substrate, the ultrastructural morphology of the mycelial hyphal cell walls differed considerably between mycelium and morphostructures. As the macromycetes passed from vegetative to generative development, the expression of the tyr1, tyr2, chi1, chi2, exg1, exg2, and exg3 genes was activated...
January 26, 2017: Journal of Microbiology / the Microbiological Society of Korea
Md Bashir Uddin, Byeong-Hoon Lee, Chamilani Nikapitiya, Jae-Hoon Kim, Tae-Hwan Kim, Hyun-Cheol Lee, Choul Goo Kim, Jong-Soo Lee, Chul-Joong Kim
No abstract text is available yet for this article.
February 2017: Journal of Microbiology / the Microbiological Society of Korea
Jung-Min Lee, Jaejung Lee, Gyeong-Hwa Nam, Byung-Sam Son, Myoung-Uoon Jang, So-Won Lee, Byung-Serk Hurh, Tae-Jip Kim
γ-Glutamyltranspeptidase (GGT) catalyzes the cleavage of γ-glutamyl compounds and the transfer of γ-glutamyl moiety to water or to amino acid/peptide acceptors. GGT can be utilized for the generation of γ-glutamyl peptides or glutamic acid, which are used as food taste enhancers. In the present study, Bacillus amyloliquefaciens SMB469 with high GGT activity was isolated from Doenjang, a traditional fermented soy food of Korea. The gene encoding GGT from B. amyloliquefaciens SMB469 (BaGGT469) was cloned from the isolate, and heterologously expressed in E...
February 2017: Journal of Microbiology / the Microbiological Society of Korea
Seyeon Shin, Hyung-Yeel Kahng
An aerobic, Gram-negative bacterium, designated strain SD70(T), was isolated from sea cucumber aquaculture farm sediment in Taean, Korea, and its taxonomic status was established by undertaking a polyphasic study. Cells of strain SD70(T) were non-motile, catalase-, and oxidase-positive, non-spore-forming, and horseshoe-shaped. Optimal growth was observed under 25-30°C, pH 7.0-8.0, and 3.0-5.0% (w/v) NaCl conditions. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain SD70(T) fell within an evolutionary group comprising species of the genus Cyclobacterium...
February 2017: Journal of Microbiology / the Microbiological Society of Korea
Ming-Feng Lin, Yun-You Lin, Chung-Yu Lan
Efflux pumps play an important role in antimicrobial resistance for Acinetobacter baumannii. However, the function of the Emr pump system and the relationship between Emr and drug resistance has not been characterized in A. baumannii. In this study, four possible groups of emr-like genes were found by searching a genome database. Among them, A1S_1772 (emrB) and A1S_1773 (emrA) were demonstrated to be co-transcribed as a single operon. Moreover, during osmotic stress, A1S_1772 showed the largest change in gene expression compared to the other emrB-like genes, and deletion of A1S_1772 (AB ΔemrB) significantly slowed cell growth in 20% sucrose...
February 2017: Journal of Microbiology / the Microbiological Society of Korea
Sugumar Ramasamy, Arumugam Arumugam, Preethy Chandran
Efficiency of Enterobacter cloacae KU923381 isolated from petroleum hydrocarbon contaminated soil was evaluated in batch culture and bioreactor mode. The isolate were screened for biofilm formation using qualitative and quantitative assays. Response surface methodology (RSM) was used to study the effect of pH, temperature, glucose concentration, and sodium chloride on diesel degradation. The predicted values for diesel oil degradation efficiency by the statistical designs are in a close agreement with experimental data (R (2) = 99...
February 2017: Journal of Microbiology / the Microbiological Society of Korea
María Clara Tarifa, Jorge Enrique Lozano, Lorena Inés Brugnoni
To clarify the interactions between a common food spoilage yeast and two pathogenic bacteria involved in outbreaks associated with fruit juices, the present paper studies the effect of the interplay of Candida krusei, collected from UF membranes, with Escherichia coli O157:H7 and Salmonella enterica in the overall process of adhesion and colonization of abiotic surfaces. Two different cases were tested: a) co-adhesion by pathogenic bacteria and yeasts, and b) incorporation of bacteria to pre-adhered C. krusei cells...
February 2017: Journal of Microbiology / the Microbiological Society of Korea
Minwook Kim, Sunwook Jeong, Sangyong Lim, Jeonggu Sim, Ho-Gun Rhie, Sung-Jae Lee
A cystine-dependent anti-oxidative stress response is characterized in Deinococcus geothermalis for the first time. Nevertheless, the same transcriptional directed Δdgeo_1985F mutant strain was revealed to have an identical phenotype to the wild-type strain, while the reverse transcriptional directed Δdgeo_1985R mutant strain was more resistant to oxidative stress at a certain concentration of H2O2 than the wild-type strain. The wild-type and mutant strains expressed equal levels of superoxide dismutase and catalase under H2O2-induced stress...
February 2017: Journal of Microbiology / the Microbiological Society of Korea
Woo Kyu Kang, Mayur Devare, Jeong-Yoon Kim
Silent information regulator 2 (Sir2), which is the founding member of the sirtuin family of proteins, is a pro-longevity factor for replicative lifespan (RLS) in Saccharomyces cerevisiae. Sir2 is required for transcriptional silencing at mating type loci, telomeres, and rDNA loci. Sir2 also represses transcription of highly expressed growth-related genes, such as PMA1 and some ribosomal protein genes. Although the Sir2 paralogues Hst1, Hst2, Hst3, and Hst4 occur in S. cerevisiae, none of them could replace the transcriptional regulation of PMA1 by Sir2 in the wild type...
February 2017: Journal of Microbiology / the Microbiological Society of Korea
Kyoung-Mi Kang, Nagendra N Mishra, Kun Taek Park, Gi-Yong Lee, Yong Ho Park, Arnold S Bayer, Soo-Jin Yang
Daptomycin (DAP) has potent activity in vitro and in vivo against both methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA) strains. DAP-resistance (DAP-R) in S. aureus has been mainly observed in MRSA strains, and has been linked to single nucleotide polymorphisms (SNPs) within the mprF gene leading to altered cell membrane (CM) phospholipid (PL) profiles, enhanced positive surface charge, and changes in CM fluidity. The current study was designed to delineate whether these same genotypic and phenotypic perturbations are demonstrated in clinically-derived DAP-R MSSA strains...
February 2017: Journal of Microbiology / the Microbiological Society of Korea
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