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Journal of Microbiology / the Microbiological Society of Korea

Jeong-Soo Park, Manbok Kim
Naturally occurring reoviruses are live replication-proficient viruses specifically infecting human cancer cells while sparing the normal counterparts. Stem cells can be highly susceptible to viral infection due to their innate high proliferation potential and other active signaling pathways of cells that might be involved in viral tropism. In the previous study, we showed that reoviruses could adversely affect murine embryonic stem cells' integrity in vitro and in vivo. Oncolytic viruses, delivered systemically face many hurdles that also impede their localization and infection of, metastatic tumors, due to a variety of immune and physical barriers...
January 2017: Journal of Microbiology / the Microbiological Society of Korea
Sung Ho Yun, Sang-Yeop Lee, Chi-Won Choi, Hayoung Lee, Hyun-Joo Ro, Sangmi Jun, Yong Min Kwon, Kae Kyoung Kwon, Sang-Jin Kim, Gun-Hwa Kim, Seung Il Kim
Novosphingobium pentaromativorans US6-1 is a Gram-negative halophilic marine bacterium able to utilize several polycyclic aromatic hydrocarbons such as phenanthrene, pyrene, and benzo[a]pyrene. In this study, using transmission electron microscopy, we confirmed that N. pentaromativorans US6-1 produces outer membrane vesicles (OMVs). N. pentaromativorans OMVs (hereafter OMVNovo) are spherical in shape, and the average diameter of OMVNovo is 25-70 nm. Proteomic analysis revealed that outer membrane proteins and periplasmic proteins of N...
January 2017: Journal of Microbiology / the Microbiological Society of Korea
Jae-Young Oh, Yong-Kuk Kwon, Bai Wei, Hyung-Kwan Jang, Suk-Kyung Lim, Cheon-Hyeon Kim, Suk-Chan Jung, Min-Su Kang
Thirty-nine human isolates of Campylobacter jejuni obtained from a national university hospital during 2007-2010 and 38 chicken isolates of C. jejuni were collected from poultry farms during 2009-2010 in South Korea were used in this study. Campylobacter genomic species and virulence-associated genes were identified by PCR. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were performed to compare their genetic relationships. All isolates were highly resistant to ciprofloxacin, nalidixic acid, and tetracycline...
January 2017: Journal of Microbiology / the Microbiological Society of Korea
Pallavi Sinha, Pradyot Prakash, Shashikant C U Patne, Shampa Anupurba, Sweety Gupta, G N Srivastava
The conventional methods for diagnosis of tubercular lymphadenitis (TBLN) such as - fine needle aspiration cytology, Ziehl-Neelsen staining and culture have limitations of low sensitivity and/or specificity. So, it becomes essential to develop a rapid, sensitive, and specific method for an early diagnosis of TBLN. Therefore, the present study was conducted to evaluate nested multiplex polymerase chain reaction (nMPCR) targeting MTP40 and IS6110 gene sequences of Mycobacterium tuberculosis and Mycobacterium tuberculosis complex, respectively in 48 successive patients of TBLN and 20 random patients with non-tubercular lymph node lesions...
January 2017: Journal of Microbiology / the Microbiological Society of Korea
Jae Ho Lee, Sae Woong Park, Young Min Kim, Jeong-Il Oh
Carbon monoxide dehydrogenase (CO-DH) in Mycobacterium sp. strain JC1 is a key enzyme for the carboxydotrophic growth, when carbon monoxide (CO) is supplied as a sole source of carbon and energy. This enzyme is also known to act as nitric oxide dehydrogenase (NO-DH) for the detoxification of NO. Several accessory genes such as cutD, cutE, cutF, cutG, cutH, and cutI, are clustered together with two copies of the CO-DH structural genes (cutB1C1A1 and cutB2C2A2) in Mycobacterium sp. strain JC1 and are well conserved in carboxydotrophic mycobacteria...
January 2017: Journal of Microbiology / the Microbiological Society of Korea
Sojin Seo, Daeyoung Kim, Wooseok Song, Jihune Heo, Minju Joo, Yeri Lim, Ji-Hyun Yeom, Kangseok Lee
RraA is a protein inhibitor of RNase E, which degrades and processes numerous RNAs in Escherichia coli. Streptomyces coelicolor also contains homologs of RNase E and RraA, RNase ES and RraAS1/RraAS2, respectively. Here, we report that, unlike other RraA homologs, RraAS1 directly interacts with the catalytic domain of RNase ES to exert its inhibitory effect. We further show that rraAS1 gene deletion in S. coelicolor results in a higher growth rate and increased production of actinorhodin and undecylprodigiosin, compared with the wild-type strain, suggesting that RraAS1-mediated regulation of RNase ES activity contributes to modulating the cellular physiology of S...
January 2017: Journal of Microbiology / the Microbiological Society of Korea
Mohammed Abd Alrahman, Sang Sun Yoon
Pseudomonas aeruginosa has been identified as an important causative agent of airway infection, mainly in cystic fibrosis. This disease is characterized by defective mucociliary clearance induced in part by mucus hyper-production. Mucin is a major component of airway mucus and is heavily O-glycosylated, with a protein backbone. Airway infection is known to be established with bacterial adhesion to mucin. However, the genes involved in mucin degradation or utilization remain elusive. In this study, we sought to provide a genetic basis of P...
January 2017: Journal of Microbiology / the Microbiological Society of Korea
Yue Ma, Qiyao Wang, Xiating Gao, Yuanxing Zhang
Fish pathogen Vibrio anguillarum, a mesophile bacterium, is usually found in estuarine and marine coastal ecosystems worldwide that pose a constant stress to local organism by its fluctuation in salinity as well as notable temperature change. Though V. anguillarum is able to proliferate while maintain its pathogenicity under low temperature (5-18°C), so far, coldadaption molecular mechanism of the bacteria is unknown. In this study, V. anguillarum was found possessing a putative glycine betaine synthesis system, which is encoded by betABI and synthesizes glycine betaine from its precursor choline...
January 2017: Journal of Microbiology / the Microbiological Society of Korea
Jeong Myeong Kim, Min-Young Choi, Jae-Woo Kim, Shin Ae Lee, Jae-Hyung Ahn, Jaekyeong Song, Seong-Hyun Kim, Hang-Yeon Weon
The gut bacterial community of wood-feeding beetles has been examined for its role on plant digestion and biocontrol method development. Monochamus alternatus and Psacothea hilaris, both belonging to the subfamily Lamiinae, are woodfeeding beetles found in eastern Asia and Europe and generally considered as destructive pests for pine and mulberry trees, respectively. However, limited reports exist on the gut bacterial communities in these species. Here, we characterized gut bacterial community compositions in larva and imago of each insect species reared with host tree logs and artificial diets as food sources...
January 2017: Journal of Microbiology / the Microbiological Society of Korea
Hee-Kyoung Kang, Cheolmin Kim, Chang Ho Seo, Yoonkyung Park
Antimicrobial peptides (AMPs) are small molecules with a broad spectrum of antibiotic activities against bacteria, yeasts, fungi, and viruses and cytotoxic activity on cancer cells, in addition to anti-inflammatory and immunomodulatory activities. Therefore, AMPs have garnered interest as novel therapeutic agents. Because of the rapid increase in drug-resistant pathogenic microorganisms, AMPs from synthetic and natural sources have been developed using alternative antimicrobial strategies. This article presents a broad analysis of patents referring to the therapeutic applications of AMPs since 2009...
January 2017: Journal of Microbiology / the Microbiological Society of Korea
Jae-Bong Lee, Seon Hwa Jeon, Seok-Gwan Choi, Hee-Young Jung, Myung Kyum Kim, Sathiyaraj Srinivasan
In this paper, a new bacterial strain designated as 16MFT21(T) is isolated from the muscle of a fish caught in the Antarctic Ocean. Strain 16MFT21(T) is a Gram-staining-positive, catalase-oxidase-positive, rod-shaped facultative-aerobic bacterium. The phylogenetic analysis that is based on the 16S-rRNA gene sequence of strain 16MFT21(T) revealed that it belongs to the genus Bacillus in the family Bacillaceae in the class Bacilli. The highest degrees of the sequence similarity of the strain 16MFT21(T) is with Bacillus licheniformis ATCC 14580(T) (96...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
Hyeonheui Ham, Sosoo Kim, Min-Hee Kim, Soohyung Lee, Sung Kee Hong, Jae-Gee Ryu, Theresa Lee
To investigate contamination of ground red pepper with fungi and mycotoxin, we obtained 30 ground red pepper samples from 15 manufacturers in the main chili-pepper-producing areas in Korea. Fungal contamination was evaluated by spreading diluted samples on potato dextrose agar plates. The total fungi counts ranged from 0 to 7.3 × 10(3) CFU/g. In the samples, the genus Aspergillus had the highest incidence, while Paecilomyces was isolated most frequently. The next most frequent genera were Rhizopus, Penicillium, Cladosporium, and Alternaria...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
Jae-Jin Lee, Yeon-Hee Lee, Su-Jin Park, Sangyong Lim, Sun-Wook Jeong, Seung-Yeol Lee, Sangkyu Park, Hyo-Won Choi, Myung Kyum Kim, Hee-Young Jung
A novel Gram-positive, oval-shaped, non-motile bacterium designated strain 16F1L(T) was isolated from sediment collected from the Han River in Seoul, Republic of Korea. Based on the 16S rRNA gene sequence (1,448 bp), this strain was identified as a member of the genus Deinococcus that belongs to the class Deinococci. Similarities in the 16S rRNA gene sequence were shown with Deinococcus daejeonensis MJ27(T) (99.0%), D. grandis DSM 3963(T) (98.1%), D. radiotolerans C1(T) (97.5%), and D. caeni Ho-08(T) (97.2%)...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
Seok-Gwan Choi, Seon Hwa Jeon, Jae-Bong Lee, Eun Sun Joo, Sangyong Lim, Hee-Young Jung, Myung Kyum Kim
Two new bacterial strains designated as Ant6(T) and Ant18 were isolated from the muscle of a fish which had been caught in the Antarctic Ocean. Both strains are Gram-stain-positive, catalase positive, oxidase negative, aerobic, and coccoid bacteria. Phylogenetic analysis based on the 16S rRNA gene sequences of strains Ant6(T) and Ant18 revealed that the strains Ant6(T) and Ant18 belong to the genus Deinococcus in the family Deinococcaceae in the class Deinococci. The highest degrees of sequence similarities of strains Ant6(T) and Ant18 were found with Deinococcus alpinitundrae LMG 24283(T) by 96...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
Md Bashir Uddin, Byeong-Hoon Lee, Chamilani Nikapitiya, Jae-Hoon Kim, Tae-Hwan Kim, Hyun-Cheol Lee, Choul Goo Kim, Jong-Soo Lee, Chul-Joong Kim
Bee venom (BV) from honey bee (Apis Melifera L.) contains at least 18 pharmacologically active components including melittin (MLT), phospholipase A2 (PLA2), and apamin etc. BV is safe for human treatments dose dependently and proven to possess different healing properties including antibacterial and antiparasitidal properties. Nevertheless, antiviral properties of BV have not well investigated. Hence, we identified the potential antiviral properties of BV and its component against a broad panel of viruses. Co-incubation of non-cytotoxic amounts of BV and MLT, the main component of BV, significantly inhibited the replication of enveloped viruses such as Influenza A virus (PR8), Vesicular Stomatitis Virus (VSV), Respiratory Syncytial Virus (RSV), and Herpes Simplex Virus (HSV)...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
Jin-Woo Lee, Kae Kyoung Kwon, Jang-Jun Bahk, Dong-Hun Lee, Hyun Sook Lee, Sung Gyun Kang, Jung-Hyun Lee
We have previously identified a sulfate methane transition zone (SMTZ) within the methane hydrate-bearing sediment in the Ulleung Basin, East Sea of Korea, and the presence of ANME-1b group in the sediment has been shown by phylogenetic analysis of a 16S rRNA gene. Herein, we describe taxonomic and functional profiling in the SMTZ sample by metagenomic analysis, comparing with that of surface sediment. Metagenomic sequences of 115 Mbp and 252 Mbp were obtained from SMTZ and surface sediments, respectively. The taxonomic profiling using BLASTX against the SEED within MG-RAST showed the prevalence of methanogens (19...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
Shin Ae Lee, Jiyoung Park, Bora Chu, Jeong Myeong Kim, Jae-Ho Joa, Mee Kyung Sang, Jaekyeong Song, Hang-Yeon Weon
The microbiome in the rhizosphere-the region surrounding plant roots-plays a key role in plant growth and health, enhancing nutrient availability and protecting plants from biotic and abiotic stresses. To assess bacterial diversity in the tomato rhizosphere, we performed two contrasting approaches: culture-dependent and -independent. In the culture-dependent approach, two culture media (Reasoner's 2A agar and soil extract agar) were supplemented with 12 antibiotics for isolating diverse bacteria from the tomato rhizosphere by inhibiting predominant bacteria...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
Aeryun Kim, Stephanie L Servetas, Jieun Kang, Jinmoon Kim, Sungil Jang, Yun Hui Choi, Hanfu Su, Yeong-Eui Jeon, Youngmin A Hong, Yun-Jung Yoo, D Scott Merrell, Jeong-Heon Cha
The array of outer membrane proteins (OMPs) found in Helicobacter pylori provides a crucial component for persistent colonization within the gastric niche. Not only does H. pylori harbor a wide number of OMPs, but these OMPs often vary across strains; this likely contributes to immune evasion, adaptation during long term colonization, and potentially differential disease progression. Previous work from our group described OMP differences among the Bab family (babA, babB, and babC) and Hom family (homA and homB) from 80 American H...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
Kyung Sook Bae, Mi Sun Kim, Ji Hee Lee, Joo Won Kang, Dae In Kim, Ji Hee Lee, Chi Nam Seong
To understand the isolation and classification state of actinobacterial species with valid names for Korean indigenous isolates, isolation source, regional origin, and taxonomic affiliation of the isolates were studied. At the time of this writing, the phylum Actinobacteria consisted of only one class, Actinobacteria, including five subclasses, 10 orders, 56 families, and 330 genera. Moreover, new taxa of this phylum continue to be discovered. Korean actinobacterial species with a valid name has been reported from 1995 as Tsukamurella inchonensis isolated from a clinical specimen...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
Jeong Myeong Kim, An-Sung Roh, Seung-Chul Choi, Eun-Jeong Kim, Moon-Tae Choi, Byung-Koo Ahn, Sun-Kuk Kim, Young-Han Lee, Jae-Ho Joa, Seong-Soo Kang, Shin Ae Lee, Jae-Hyung Ahn, Jaekyeong Song, Hang-Yeon Weon
Soil microorganisms play an essential role in soil ecosystem processes such as organic matter decomposition, nutrient cycling, and plant nutrient availability. The land use for greenhouse cultivation has been increasing continuously, which involves an intensive input of agricultural materials to enhance productivity; however, relatively little is known about bacterial communities in greenhouse soils. To assess the effects of environmental factors on the soil bacterial diversity and community composition, a total of 187 greenhouse soil samples collected across Korea were subjected to bacterial 16S rRNA gene pyrosequencing analysis...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
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