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Cell Biochemistry and Biophysics

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https://www.readbyqxmd.com/read/28986856/spin-lattice-relaxation-epr-po2-images-may-direct-the-location-of-radiation-tumor-boosts-to-enhance-tumor-cure
#1
Boris Epel, Martyna Krzykawska-Serda, Victor Tormyshev, Matthew C Maggio, Eugene D Barth, Charles A Pelizzari, Howard J Halpern
Radiation treatment success and high tumor oxygenation and success have been known to be highly correlated. This suggests that radiation therapy guided by images of tumor regions with low oxygenation, oxygen-guided radiation therapy (OGRT) may be a promising enhancement of cancer radiation treatment. Before applying the technique to human subjects, OGRT needs to be tested in animals, most easily in rodents. Electron paramagnetic resonance imaging provides quantitative maps of tissue and tumor oxygen in rodents with 1 mm spatial resolution and 1 torr pO2 resolution at low oxygen levels...
October 6, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28975596/erratum-to-hypoxia-augments-increased-hif-1a-and-reduced-survival-protein-p-akt-in-gelsolin-gsn-dependent-cardiomyoblast-cell-apoptosis
#2
Yu-Lan Yeh, Wei-Syun Hu, Wei-Jen Ting, Chia-Yao Shen, Hsi-Hsien Hsu, Li-Chin Chung, Chuan-Chou Tu, Sheng-Huang Chang, Cecilia-Hsuan Day, Yuhsin Tsai, Chih-Yang Huang
No abstract text is available yet for this article.
October 3, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28952073/cysteine-to-serine-conversion-at-111th-position-renders-the-disaggregation-and-retains-the-stabilization-of-detrimental-sod1-a4v-mutant-against-amyotrophic-lateral-sclerosis-in-human-a-discrete-molecular-dynamics-study
#3
E Srinivasan, R Rajasekaran
Protein aggregation is a hallmark of various neurodegenerative disorders, such as amyotrophic lateral sclerosis (ALS) in humans. Mutations in Cu/Zn superoxide dismutase (SOD1) protein were found to be a prominent cause behind the majority of the familial ALS cases with abnormal protein aggregates. Herein, we report the biophysical characterization of the beneficial mutation C111S that stabilizes the SOD1 harboring A4V mutation, one of the most lethal diseases causing mutant that leads to protein destabilization and aggregation...
September 26, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28948569/blood-free-radicals-concentration-determined-by-electron-paramagnetic-resonance-spectroscopy-and-delayed-cerebral-ischemia-occurrence-in-patients-with-aneurysmal-subarachnoid-hemorrhage
#4
Grzywna Ewelina, Stachura Krzysztof, Moskala Marek, Kruczala Krzysztof
Pathophysiology of delayed cerebral ischemia and cerebral vasospasm following aneurysmal subarachnoid hemorrhage is still poorly recognized, however free radicals are postulated as one of the crucial players. This study was designed to scrutinize whether the concentration of free radicals in the peripheral venous blood is related to the occurrence of delayed cerebral ischemia associated with cerebral vasospasm. Twenty-four aneurysmal subarachnoid hemorrhage patients and seven patients with unruptured intracranial aneurysm (control group) have been studied...
September 25, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28942575/a-contact-based-method-for-differentiation-of-human-mesenchymal-stem-cells-into-an-endothelial-cell-phenotype
#5
Binata Joddar, Shweta Anil Kumar, Alok Kumar
Adult stem cells such as mesenchymal stem cells (MSC) are known to possess the ability to augment neovascularization processes and are thus widely popular as an autologous source of progenitor cells. However there is a huge gap in our current knowledge of mechanisms involved in differentiating MSC into endothelial cells (EC), essential for lining engineered blood vessels. To fill up this gap, we attempted to differentiate human MSC into EC, by culturing the former onto chemically fixed layers of EC or its ECM, respectively...
September 23, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28871484/epr-oximetry-sensor-developing-a-tam-derivative-for-in-vivo-studies
#6
Agnieszka Boś-Liedke, Magdalena Walawender, Anna Woźniak, Dorota Flak, Jacek Gapiński, Stefan Jurga, Małgorzata Kucińska, Adam Plewiński, Marek Murias, Marwa Elewa, Lisa Lampp, Peter Imming, Krzysztof Tadyszak
Oxygenation is one of the most important physiological parameters of biological systems. Low oxygen concentration (hypoxia) is associated with various pathophysiological processes in different organs. Hypoxia is of special importance in tumor therapy, causing poor response to treatment. Triaryl methyl (TAM) derivative radicals are commonly used in electron paramagnetic resonance (EPR) as sensors for quantitative spatial tissue oxygen mapping. They are also known as magnetic resonance imaging (MRI) contrast agents and fluorescence imaging compounds...
September 4, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28852971/identification-of-phytochemicals-targeting-c-met-kinase-domain-using-consensus-docking-and-molecular-dynamics-simulation-studies
#7
Shima Aliebrahimi, Shideh Montasser Kouhsari, Seyed Nasser Ostad, Seyed Shahriar Arab, Leila Karami
c-Met receptor tyrosine kinase is a proto-oncogene whose aberrant activation is attributed to a lower rate of survival in most cancers. Natural product-derived inhibitors known as "fourth generation inhibitors" constitute more than 60% of anticancer drugs. Furthermore, consensus docking approach has recently been introduced to augment docking accuracy and reduce false positives during a virtual screening. In order to obtain novel small-molecule Met inhibitors, consensus docking approach was performed using Autodock Vina and Autodock 4...
August 29, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28849322/epr-technology-as-sensitive-method-for-oxidative-stress-detection-in-primary-and-secondary-keratinocytes-induced-by-two-selected-nanoparticles
#8
S B Lohan, S Ahlberg, A Mensch, D Höppe, M Giulbudagian, M Calderón, S Grether-Beck, J Krutmann, J Lademann, M C Meinke
Exogenous factors can cause an imbalance in the redox state of biological systems, promoting the development of oxidative stress, especially reactive oxygen species (ROS). To monitor the intensity of ROS production in secondary keratinocytes (HaCaT) by diesel exhaust particles and thermoresponsive nanogels (tNG), electron paramagnetic resonance (EPR) spectroscopy after 1 and 24 h of incubation, respectively, was applied. Their cytotoxicity was analyzed by a cell viability assay (XTT). For tNG an increase in the cell viability and ROS production of 10% was visible after 24 h, whereas 1 h showed no effect...
August 28, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28831668/fish-collagen-hydrolysates-modulate-cartilage-metabolism
#9
Kanchanit Boonmaleerat, Orawan Wanachewin, Thanyaluck Phitak, Peraphan Pothacharoen, Prachya Kongtawelert
Osteoarthritis is a degenerative joint disease in which interleukin-1β plays a major role in the inflammatory process. Administration of collagen hydrolysate was an optional treatment of osteoarthritis. Fish has become an interesting source of collagen hydrolysate because of religious reason and there is no risk from mad cow disease. However, the effects of different sizes of fish collagen hydrolysate on cartilage and chondrocyte metabolism have not been well studied yet. This study examined the effect of different sizes of fish collagen hydrolysate on cartilage metabolism...
August 22, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28822069/characterization-of-ofloxacin-interaction-with-mutated-a91v-quinolone-resistance-determining-region-of-dna-gyrase-in-mycobacterium-leprae-through-computational-simulation
#10
J Nisha, V Shanthi
Mycobacterium leprae, the causal agent of leprosy is non-cultivable in vitro. Thus, the assessment of antibiotic activity against Mycobacterium leprae depends primarily upon the time-consuming mouse footpad system. The GyrA protein of Mycobacterium leprae is the target of the antimycobacterial drug, Ofloxacin. In recent times, the GyrA mutation (A91V) has been found to be resistant to Ofloxacin. This phenomenon has necessitated the development of new, long-acting antimycobacterial compounds. The underlying mechanism of drug resistance is not completely known...
August 18, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28770488/identification-and-characterization-of-functional-single-nucleotide-polymorphisms-snps-in-axin-1-gene-a-molecular-dynamics-approach
#11
Imran Khan, Irfan A Ansari, Pratichi Singh, J Febin Prabhu Dass, Fahad Khan
Wnt signaling pathway has been reported to play crucial role in intestinal crypt formation and deregulation of this pathway is responsible for colorectal cancer initiation and progression. Axin 1, a scaffold protein, play pivotal role in the regulation of Wnt/β-catenin signaling pathway and has been found to be mutated in several cancers; primarily in colon cancer. Considering its crucial role, a structural and functional analysis of missense mutations in Axin 1 gene was performed in this study. Initially, one hundred non-synonymous single nucleotide polymorphisms in the coding regions of Axin 1 gene were selected for in silico analysis...
August 2, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28756483/identification-of-structure-stabilizing-interactions-in-enzymes-a-novel-mechanism-to-impact-enzyme-activity
#12
Marisol Serrano, Veronica Gonzalez, Supriyo Ray, Maria D Chavez, Mahesh Narayan
Cruzain, a cysteine protease in the cathepsin family, is pivotal to the life-cycle of Trypanosoma cruzi, the etiological agent in Chagas disease. Current inhibitors of cruzain suffer from drawbacks involving gastrointestinal and neurological side effects and as a result have spurred the search for alternative anti-trypanocidals. Through sequence alignment studies and intra-residue interaction analysis of the pro-protein of cruzain (pro-cruzain), we have identified a host of non-active site residues that are conserved among the cathepsins...
July 29, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28756482/epr-oximetry-of-cetuximab-treated-head-and-neck-tumours-in-a-mouse-model
#13
H Gustafsson, A Kale, A Dasu, A Lund, P-H Edqvist, K Roberg
Head and neck squamous cell carcinoma (HNSCC) tumours are associated with high mortality despite advances in therapy. The monoclonal antibody cetuximab (Erbitux(®)) has been approved for the treatment of advanced HNSCC. However, only a subset of HNSC patients receiving cetuximab actually responds to treatment, underlining the need for a means to tailor treatments of individual patients. The aim of the present study was to investigate the effect of cetuximab treatment on tumour growth, on tumour partial oxygen pressure as measured by LiPc electron paramagnetic resonance oximetry and on the expression of proteins involved in tumour growth, metabolism and hypoxia...
July 29, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726180/epr-characterization-of-dinitrosyl-iron-complexes-with-thiol-containing-ligands-as-an-approach-to-their-identification-in-biological-objects-an-overview
#14
REVIEW
Anatoly F Vanin
The overview demonstrates how the use of only one physico-chemical approach, viz., the electron paramagnetic resonance method, allowed detection and identification of dinitrosyl iron complexes with thiol-containing ligands in various animal and bacterial cells. These complexes are formed in biological objects in the paramagnetic (electron paramagnetic resonance-active) mononuclear and diamagnetic (electron paramagnetic resonance-silent) binuclear forms and control the activity of nitrogen monoxide, one of the most universal regulators of metabolic processes in the organism...
July 19, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726179/methionine-and-or-methionine-sulfoxide-alter-ectoenzymes-activities-in-lymphocytes-and-inflammatory-parameters-in-serum-from-young-rats-acute-and-chronic-effects
#15
Mayara Sandrielly Pereira Soares, Marcelo Zanusso Costa, Tatiane Morgana da Silva, Marta Gazal, Carlus Augustu Tavares do Couto, Gabriela Nogueira Debom, Rodrigo Rodrigues, Juliana Hofstätter Azambuja, Emerson André Casali, Cesar Eduardo Jacintho Moritz, Marta Frescura Duarte, Elizandra Braganhol, Francieli Moro Stefanello, Roselia Maria Spanevello
In this study we investigated the effect of acute and chronic treatment with Met and/or methionine sulfoxide (MetO) on ectonucleotidases and cholinesterases activities from lymphocytes and purine derivatives compounds, C-protein reactive, interleukin-10, interleukin-6, and tumor necrosis factor-α levels in serum of young rats. Adenosine triphosphate hydrolysis was decreased in lymphocytes 1 h after treatment by MetO and Met + MetO. However, adenosine triphosphate and adenosine diphosphate hydrolysis in lymphocytes was increased in the groups MetO and Met + MetO and adenosine deaminase activity was increased in MetO 3 h after the treatment...
July 19, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28687906/development-of-the-implantable-resonator-system-for-clinical-epr-oximetry
#16
Rose M Caston, Wilson Schreiber, Huagang Hou, Benjamin B Williams, Eunice Y Chen, Philip E Schaner, Lesley A Jarvis, Ann Barry Flood, Sergey V Petryakov, Maciej M Kmiec, Periannan Kuppusamy, Harold M Swartz
Hypoxic tumors are more resistant to radiotherapy and chemotherapy, which decreases the efficacy of these common forms of treatment. We have been developing implantable paramagnetic particulates to measure oxygen in vivo using electron paramagnetic resonance. Once implanted, oxygen can be measured repeatedly and non-invasively in superficial tissues (<3 cm deep), using an electron paramagnetic resonance spectrometer and an external surface-loop resonator. To significantly extend the clinical applications of electron paramagnetic resonance oximetry, we developed an implantable resonator system to obtain measurements at deeper sites...
July 7, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28660427/cholesterol-bilayer-domains-in-the-eye-lens-health-a-review
#17
Justyna Widomska, Witold K Subczynski, Laxman Mainali, Marija Raguz
The most unique biochemical characteristic of the eye lens fiber cell plasma membrane is its extremely high cholesterol content, the need for which is still unclear. It is evident, however, that the disturbance of Chol homeostasis may result in damages associated with cataracts. Electron paramagnetic resonance methods allow discrimination of two types of lipid domains in model membranes overloaded with Chol, namely, phospholipid-cholesterol domains and pure Chol bilayer domains. These domains are also detected in human lens lipid membranes prepared from the total lipids extracted from lens cortices and nuclei of donors from different age groups...
June 29, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28660426/recent-developments-in-the-probes-and-assays-for-measurement-of-the-activity-of-nadph-oxidases
#18
Jacek Zielonka, Micael Hardy, Radosław Michalski, Adam Sikora, Monika Zielonka, Gang Cheng, Olivier Ouari, Radosław Podsiadły, Balaraman Kalyanaraman
NADPH oxidases are a family of enzymes capable of transferring electrons from NADPH to molecular oxygen. A major function of NADPH oxidases is the activation of molecular oxygen into reactive oxygen species. Increased activity of NADPH oxidases has been implicated in various pathologies, including cardiovascular disease, neurological dysfunction, and cancer. Thus, NADPH oxidases have been identified as a viable target for the development of novel therapeutics exhibiting inhibitory effects on NADPH oxidases...
June 29, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28646414/dynamic-meso-scale-anchorage-of-gpi-anchored-receptors-in-the-plasma-membrane-prion-protein-vs-thy1
#19
Yuri L Nemoto, Roger J Morris, Hiroko Hijikata, Taka A Tsunoyama, Akihiro C E Shibata, Rinshi S Kasai, Akihiro Kusumi, Takahiro K Fujiwara
The central mechanism for the transmission of the prion protein misfolding is the structural conversion of the normal cellular prion protein to the pathogenic misfolded prion protein, by the interaction with misfolded prion protein. This process might be enhanced due to the homo-dimerization/oligomerization of normal prion protein. However, the behaviors of normal prion protein in the plasma membrane have remained largely unknown. Here, using single fluorescent-molecule imaging, we found that both prion protein and Thy1, a control glycosylphosphatidylinositol-anchored protein, exhibited very similar intermittent transient immobilizations lasting for a few seconds within an area of 24...
June 24, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28646413/integrated-in-silico-in-vitro-identification-and-characterization-of-the-sh3-mediated-interaction-between-human-pttg-and-its-cognate-partners-in-medulloblastoma
#20
Jiangang Liu, Dapeng Wang, Yanyan Li, Hui Yao, Nan Zhang, Xuewen Zhang, Fangping Zhong, Yulun Huang
The human pituitary tumor-transforming gene is an oncogenic protein which serves as a central hub in the cellular signaling network of medulloblastoma. The protein contains two vicinal PxxP motifs at its C terminus that are potential binding sites of peptide-recognition SH3 domains. Here, a synthetic protocol that integrated in silico analysis and in vitro assay was described to identify the SH3-binding partners of pituitary tumor-transforming gene in the gene expression profile of medulloblastoma. In the procedure, a variety of structurally diverse, non-redundant SH3 domains with high gene expression in medulloblastoma were compiled, and their three-dimensional structures were either manually retrieved from the protein data bank database or computationally modeled through bioinformatics technique...
June 24, 2017: Cell Biochemistry and Biophysics
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