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Cell Biochemistry and Biophysics

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https://www.readbyqxmd.com/read/28822069/characterization-of-ofloxacin-interaction-with-mutated-a91v-quinolone-resistance-determining-region-of-dna-gyrase-in-mycobacterium-leprae-through-computational-simulation
#1
J Nisha, V Shanthi
Mycobacterium leprae, the causal agent of leprosy is non-cultivable in vitro. Thus, the assessment of antibiotic activity against Mycobacterium leprae depends primarily upon the time-consuming mouse footpad system. The GyrA protein of Mycobacterium leprae is the target of the antimycobacterial drug, Ofloxacin. In recent times, the GyrA mutation (A91V) has been found to be resistant to Ofloxacin. This phenomenon has necessitated the development of new, long-acting antimycobacterial compounds. The underlying mechanism of drug resistance is not completely known...
August 18, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28770488/identification-and-characterization-of-functional-single-nucleotide-polymorphisms-snps-in-axin-1-gene-a-molecular-dynamics-approach
#2
Imran Khan, Irfan A Ansari, Pratichi Singh, J Febin Prabhu Dass, Fahad Khan
Wnt signaling pathway has been reported to play crucial role in intestinal crypt formation and deregulation of this pathway is responsible for colorectal cancer initiation and progression. Axin 1, a scaffold protein, play pivotal role in the regulation of Wnt/β-catenin signaling pathway and has been found to be mutated in several cancers; primarily in colon cancer. Considering its crucial role, a structural and functional analysis of missense mutations in Axin 1 gene was performed in this study. Initially, one hundred non-synonymous single nucleotide polymorphisms in the coding regions of Axin 1 gene were selected for in silico analysis...
August 2, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28756483/identification-of-structure-stabilizing-interactions-in-enzymes-a-novel-mechanism-to-impact-enzyme-activity
#3
Marisol Serrano, Veronica Gonzalez, Supriyo Ray, Maria D Chavez, Mahesh Narayan
Cruzain, a cysteine protease in the cathepsin family, is pivotal to the life-cycle of Trypanosoma cruzi, the etiological agent in Chagas disease. Current inhibitors of cruzain suffer from drawbacks involving gastrointestinal and neurological side effects and as a result have spurred the search for alternative anti-trypanocidals. Through sequence alignment studies and intra-residue interaction analysis of the pro-protein of cruzain (pro-cruzain), we have identified a host of non-active site residues that are conserved among the cathepsins...
July 29, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28756482/epr-oximetry-of-cetuximab-treated-head-and-neck-tumours-in-a-mouse-model
#4
H Gustafsson, A Kale, A Dasu, A Lund, P-H Edqvist, K Roberg
Head and neck squamous cell carcinoma (HNSCC) tumours are associated with high mortality despite advances in therapy. The monoclonal antibody cetuximab (Erbitux(®)) has been approved for the treatment of advanced HNSCC. However, only a subset of HNSC patients receiving cetuximab actually responds to treatment, underlining the need for a means to tailor treatments of individual patients. The aim of the present study was to investigate the effect of cetuximab treatment on tumour growth, on tumour partial oxygen pressure as measured by LiPc electron paramagnetic resonance oximetry and on the expression of proteins involved in tumour growth, metabolism and hypoxia...
July 29, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726180/epr-characterization-of-dinitrosyl-iron-complexes-with-thiol-containing-ligands-as-an-approach-to-their-identification-in-biological-objects-an-overview
#5
REVIEW
Anatoly F Vanin
The overview demonstrates how the use of only one physico-chemical approach, viz., the electron paramagnetic resonance method, allowed detection and identification of dinitrosyl iron complexes with thiol-containing ligands in various animal and bacterial cells. These complexes are formed in biological objects in the paramagnetic (electron paramagnetic resonance-active) mononuclear and diamagnetic (electron paramagnetic resonance-silent) binuclear forms and control the activity of nitrogen monoxide, one of the most universal regulators of metabolic processes in the organism...
July 19, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28726179/methionine-and-or-methionine-sulfoxide-alter-ectoenzymes-activities-in-lymphocytes-and-inflammatory-parameters-in-serum-from-young-rats-acute-and-chronic-effects
#6
Mayara Sandrielly Pereira Soares, Marcelo Zanusso Costa, Tatiane Morgana da Silva, Marta Gazal, Carlus Augustu Tavares do Couto, Gabriela Nogueira Debom, Rodrigo Rodrigues, Juliana Hofstätter Azambuja, Emerson André Casali, Cesar Eduardo Jacintho Moritz, Marta Frescura Duarte, Elizandra Braganhol, Francieli Moro Stefanello, Roselia Maria Spanevello
In this study we investigated the effect of acute and chronic treatment with Met and/or methionine sulfoxide (MetO) on ectonucleotidases and cholinesterases activities from lymphocytes and purine derivatives compounds, C-protein reactive, interleukin-10, interleukin-6, and tumor necrosis factor-α levels in serum of young rats. Adenosine triphosphate hydrolysis was decreased in lymphocytes 1 h after treatment by MetO and Met + MetO. However, adenosine triphosphate and adenosine diphosphate hydrolysis in lymphocytes was increased in the groups MetO and Met + MetO and adenosine deaminase activity was increased in MetO 3 h after the treatment...
July 19, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28687906/development-of-the-implantable-resonator-system-for-clinical-epr-oximetry
#7
Rose M Caston, Wilson Schreiber, Huagang Hou, Benjamin B Williams, Eunice Y Chen, Philip E Schaner, Lesley A Jarvis, Ann Barry Flood, Sergey V Petryakov, Maciej M Kmiec, Periannan Kuppusamy, Harold M Swartz
Hypoxic tumors are more resistant to radiotherapy and chemotherapy, which decreases the efficacy of these common forms of treatment. We have been developing implantable paramagnetic particulates to measure oxygen in vivo using electron paramagnetic resonance. Once implanted, oxygen can be measured repeatedly and non-invasively in superficial tissues (<3 cm deep), using an electron paramagnetic resonance spectrometer and an external surface-loop resonator. To significantly extend the clinical applications of electron paramagnetic resonance oximetry, we developed an implantable resonator system to obtain measurements at deeper sites...
July 7, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28660427/cholesterol-bilayer-domains-in-the-eye-lens-health-a-review
#8
Justyna Widomska, Witold K Subczynski, Laxman Mainali, Marija Raguz
The most unique biochemical characteristic of the eye lens fiber cell plasma membrane is its extremely high cholesterol content, the need for which is still unclear. It is evident, however, that the disturbance of Chol homeostasis may result in damages associated with cataracts. Electron paramagnetic resonance methods allow discrimination of two types of lipid domains in model membranes overloaded with Chol, namely, phospholipid-cholesterol domains and pure Chol bilayer domains. These domains are also detected in human lens lipid membranes prepared from the total lipids extracted from lens cortices and nuclei of donors from different age groups...
June 29, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28660426/recent-developments-in-the-probes-and-assays-for-measurement-of-the-activity-of-nadph-oxidases
#9
Jacek Zielonka, Micael Hardy, Radosław Michalski, Adam Sikora, Monika Zielonka, Gang Cheng, Olivier Ouari, Radosław Podsiadły, Balaraman Kalyanaraman
NADPH oxidases are a family of enzymes capable of transferring electrons from NADPH to molecular oxygen. A major function of NADPH oxidases is the activation of molecular oxygen into reactive oxygen species. Increased activity of NADPH oxidases has been implicated in various pathologies, including cardiovascular disease, neurological dysfunction, and cancer. Thus, NADPH oxidases have been identified as a viable target for the development of novel therapeutics exhibiting inhibitory effects on NADPH oxidases...
June 29, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28646414/dynamic-meso-scale-anchorage-of-gpi-anchored-receptors-in-the-plasma-membrane-prion-protein-vs-thy1
#10
Yuri L Nemoto, Roger J Morris, Hiroko Hijikata, Taka A Tsunoyama, Akihiro C E Shibata, Rinshi S Kasai, Akihiro Kusumi, Takahiro K Fujiwara
The central mechanism for the transmission of the prion protein misfolding is the structural conversion of the normal cellular prion protein to the pathogenic misfolded prion protein, by the interaction with misfolded prion protein. This process might be enhanced due to the homo-dimerization/oligomerization of normal prion protein. However, the behaviors of normal prion protein in the plasma membrane have remained largely unknown. Here, using single fluorescent-molecule imaging, we found that both prion protein and Thy1, a control glycosylphosphatidylinositol-anchored protein, exhibited very similar intermittent transient immobilizations lasting for a few seconds within an area of 24...
June 24, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28646413/integrated-in-silico-in-vitro-identification-and-characterization-of-the-sh3-mediated-interaction-between-human-pttg-and-its-cognate-partners-in-medulloblastoma
#11
Jiangang Liu, Dapeng Wang, Yanyan Li, Hui Yao, Nan Zhang, Xuewen Zhang, Fangping Zhong, Yulun Huang
The human pituitary tumor-transforming gene is an oncogenic protein which serves as a central hub in the cellular signaling network of medulloblastoma. The protein contains two vicinal PxxP motifs at its C terminus that are potential binding sites of peptide-recognition SH3 domains. Here, a synthetic protocol that integrated in silico analysis and in vitro assay was described to identify the SH3-binding partners of pituitary tumor-transforming gene in the gene expression profile of medulloblastoma. In the procedure, a variety of structurally diverse, non-redundant SH3 domains with high gene expression in medulloblastoma were compiled, and their three-dimensional structures were either manually retrieved from the protein data bank database or computationally modeled through bioinformatics technique...
June 24, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28623516/erratum-to-a-simple-analytical-approximation-to-an-inhomogeneously-broadened-dispersion-spectrum-application-to-absorption-dispersion-admixtures
#12
Barney L Bales
No abstract text is available yet for this article.
June 16, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28577243/review-on-the-biological-mechanisms-associated-with-depo-provera-and-hiv-1-risk-acquisition-in-women
#13
REVIEW
Funanani Takalani, Ndumiso N Mhlongo, Suri Moonsamy, Mahmoud E S Soliman
Women constitute more than 50% out of millions of individuals infected with HIV-1, the major causative agent of acquired immune deficiency syndrome. About 40% of HIV-1 infections have been reported to initiate in the female reproductive tract. However, the mechanisms through which these infections are spread are poorly understood; hence, there is now a major concern in women who use long acting injectable hormonal contraceptives, particularly Depo-Provera and an increase of HIV-1 risk acquisition. Based on literature, Depo-Provera has an affinity for both the glucocorticoid receptor and the progesterone receptor in the female reproductive tract...
June 3, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28555359/broadband-w-band-rapid-frequency-sweep-considerations-for-fourier-transform-epr
#14
Robert A Strangeway, James S Hyde, Theodore G Camenisch, Jason W Sidabras, Richard R Mett, James R Anderson, Joseph J Ratke, Witold K Subczynski
A multi-arm W-band (94 GHz) electron paramagnetic resonance spectrometer that incorporates a loop-gap resonator with high bandwidth is described. A goal of the instrumental development is detection of free induction decay following rapid sweep of the microwave frequency across the spectrum of a nitroxide radical at physiological temperature, which is expected to lead to a capability for Fourier transform electron paramagnetic resonance. Progress toward this goal is a theme of the paper. Because of the low Q-value of the loop-gap resonator, it was found necessary to develop a new type of automatic frequency control, which is described in an appendix...
May 29, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28417230/retraction-note-to-vegf-silencing-inhibits-human-osteosarcoma-angiogenesis-and-promotes-cell-apoptosis-via-pi3k-akt-signaling-pathway
#15
Jian Zhao, Zi-Ru Zhang, Na Zhao, Bao-An Ma, Qing-Yu Fan
No abstract text is available yet for this article.
June 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28315132/nitroxide-radicals-synthesis-and-functional-bio-nanomaterials-an-introduction
#16
EDITORIAL
Alex I Smirnov, Lawrence J Berliner
No abstract text is available yet for this article.
June 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28271339/spin-probe-multi-frequency-epr-study-of-unprocessed-cotton-fibers
#17
Antonin Marek, Maxim A Voinov, Alex I Smirnov
Known since the ancient times, cotton continues to be one of the essential materials for the human civilization. Cotton fibers are almost pure cellulose and contain both crystalline and amorphous nanodomains with different physicochemical properties. While understanding of interactions between the individual cellulose chains within the crystalline phase is important from a perspective of mechanical properties, studies of the amorphous phase lead to characterization of the essential transport parameters, such as solvent diffusion, dyeing, drug release, and toxin absorption, as well as more complex processes of enzymatic degradation...
June 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28210984/effect-of-solution-ionic-strength-on-the-pka-of-the-nitroxide-ph-epr-probe-2-2-3-4-5-5-hexamethylimidazolidin-1-oxyl
#18
Kaleigh Margita, Maxim A Voinov, Alex I Smirnov
Spin probe and spin labeling Electron Paramagnetic Resonance methods are indispensable research tools for solving a wide range of bioanalytical problems-from measuring microviscosity and polarity of phase-separated liquids to oxygen concentrations in tissues. One of the emerging uses of spin probes are the studies of proton transfer-related and surface electrostatic phenomena. The latter Electron Paramagnetic Resonance methods rely on molecular probes containing an additional functionality capable of reversible ionization (protonation, in particular) in the immediate proximity to an Electron Paramagnetic Resonance-active reporter group, such as (N-O(•)) for nitroxides...
June 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27815780/interfacial-electrostatic-properties-of-hydrated-mesoporous-and-nanostructured-alumina-powders-by-spin-labeling-epr
#19
Elena G Kovaleva, Leonid S Molochnikov, Darya P Stepanova, Alexander V Pestov, Dmitrii G Trofimov, Igor A Kirilyuk, Alex I Smirnov
Acid-base equilibria and interfacial electrostatic properties of hydrated mesoporous and nanostructured alumina powders are determining factors for the use of these materials in heterogeneous catalysis and as a sorption media for filtration and chromatographic applications including life sciences. Here spin probe electron paramagnetic resonance spectroscopy of pH-sensitive nitroxides was employed to evaluate the surface charge and interfacial acid-base equilibria at the pore surface of mesoporous powders of α-Al2O3, γ-Al2O3, Al2O3 × nH2O, and basic γ-Al2O3 and nanostructured Al2O3 in the form of pristine materials and modified with aluminum-tri-sec-butoxide, hydroxyaluminum glycerate, and several phospholipids...
June 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27709467/biological-relevance-of-free-radicals-and-nitroxides
#20
REVIEW
Christopher Prescott, Steven E Bottle
Nitroxides are stable, kinetically-persistent free radicals which have been successfully used in the study and intervention of oxidative stress, a critical issue pertaining to cellular health which results from an imbalance in the levels of damaging free radicals and redox-active species in the cellular environment. This review gives an overview of some of the biological processes that produce radicals and other reactive oxygen species with relevance to oxidative stress, and then discusses interactions of nitroxides with these species in terms of the use of nitroxides as redox-sensitive probes and redox-active therapeutic agents...
June 2017: Cell Biochemistry and Biophysics
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