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Cell Biochemistry and Biophysics

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https://www.readbyqxmd.com/read/28332021/female-hormone-17%C3%AE-estradiol-downregulated-mmp-2-expression-and-upregulated-a1pi-expression-in-human-corneal-stromal-cells
#1
Hongbo Yin, Qi Wan, Yan Tian, Bo Zhao, Yingping Deng
Collagens are essential for cornea functions. In non-ocular tissues, it has been demonstrated that sex hormones modulate the collagen remodeling. In this study, we investigated whether the primary female hormone 17β-estradiol plays a role in the expressions of matrix metalloproteinases and proteinase inhibitors in cultured human corneal stromal cells. We found that 17β-estradiol treatment significantly reduced the matrix metalloproteinase-2 mRNA in human corneal stromal cells as well as the matrix metalloproteinase-2 proteins, while the matrix metalloproteinase-9 mRNA level was not significantly altered...
March 23, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28321613/idh-inhibiting-small-molecule-dtdq-inhibits-migration-and-invasion-of-a549-human-non-small-cell-lung-cancer-cells-via-sequential-inactivation-of-erk-and-p38-signaling-pathways
#2
Soonchan Park, Jongsung Lee, Sang Yeol Lee
Migration and invasion are two core processes during cancer metastasis, and several signaling pathways have been shown to be involved. A key regulator of metastasis is the mitogen-activated protein kinase signaling pathway. Here, we report that the small molecule, 6,7-dimethyl-4-(3,4,5-trimethoxyphenyl)-3,4-dihydroquinolin-2(1H)-one, inhibited isocitrate dehydrogenase activity and had anti-metastatic effects in A549 human non-small-cell lung cancer cells. 6,7-dimethyl-4-(3,4,5-trimethoxyphenyl)-3,4-dihydroquinolin-2(1H)-one induced sequential inactivation of the extracellular signal-regulated kinases and p38 signaling pathways, both representative mitogen-activated protein kinase family members...
March 21, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28315132/nitroxide-radicals-synthesis-and-functional-bio-nanomaterials-an-introduction
#3
EDITORIAL
Alex I Smirnov, Lawrence J Berliner
No abstract text is available yet for this article.
March 17, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28281231/intrinsically-disordered-regions-in-serum-albumin-what-are-they-for
#4
REVIEW
Ekaterina A Litus, Sergei E Permyakov, Vladimir N Uversky, Eugene A Permyakov
Serum albumin is a major plasma protein in mammalian blood. The importance of this protein lies in its roles in both bioregulation and transport phenomena. Serum albumin binds various metal ions and participates in the transport and storage of fatty acids, bilirubin, steroids amino acids, and many other ligands, usually with regions of hydrophobic surface. Although the primary role of serum albumin is to transport various ligand, its versatile binding capacities and high concentration mean that it can assume a number of additional functions...
March 10, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28281230/identifying-the-interaction-of-vancomycin-with-novel-ph-responsive-lipids-as-antibacterial-biomaterials-via-accelerated-molecular-dynamics-and-binding-free-energy-calculations
#5
Shaimaa Ahmed, Suresh B Vepuri, Mahantesh Jadhav, Rahul S Kalhapure, Thirumala Govender
Nano-drug delivery systems have proven to be an efficient formulation tool to overcome the challenges with current antibiotics therapy and resistance. A series of pH-responsive lipid molecules were designed and synthesized for future liposomal formulation as a nano-drug delivery system for vancomycin at the infection site. The structures of these lipids differ from each other in respect of hydrocarbon tails: Lipid1, 2, 3 and 4 have stearic, oleic, linoleic, and linolenic acid hydrocarbon chains, respectively...
March 9, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28271339/spin-probe-multi-frequency-epr-study-of-unprocessed-cotton-fibers
#6
Antonin Marek, Maxim A Voinov, Alex I Smirnov
Known since the ancient times, cotton continues to be one of the essential materials for the human civilization. Cotton fibers are almost pure cellulose and contain both crystalline and amorphous nanodomains with different physicochemical properties. While understanding of interactions between the individual cellulose chains within the crystalline phase is important from a perspective of mechanical properties, studies of the amorphous phase lead to characterization of the essential transport parameters, such as solvent diffusion, dyeing, drug release, and toxin absorption, as well as more complex processes of enzymatic degradation...
March 7, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28210984/effect-of-solution-ionic-strength-on-the-pka-of-the-nitroxide-ph-epr-probe-2-2-3-4-5-5-hexamethylimidazolidin-1-oxyl
#7
Kaleigh Margita, Maxim A Voinov, Alex I Smirnov
Spin probe and spin labeling Electron Paramagnetic Resonance methods are indispensable research tools for solving a wide range of bioanalytical problems-from measuring microviscosity and polarity of phase-separated liquids to oxygen concentrations in tissues. One of the emerging uses of spin probes are the studies of proton transfer-related and surface electrostatic phenomena. The latter Electron Paramagnetic Resonance methods rely on molecular probes containing an additional functionality capable of reversible ionization (protonation, in particular) in the immediate proximity to an Electron Paramagnetic Resonance-active reporter group, such as (N-O(•)) for nitroxides...
February 17, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28197900/letter-from-the-editor-in-chief-2017
#8
EDITORIAL
Lawrence J Berliner
No abstract text is available yet for this article.
February 15, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28144904/vp40-of-the-ebola-virus-as-a-target-for-ebov-therapy-comprehensive-conformational-and-inhibitor-binding-landscape-from-accelerated-molecular-dynamics
#9
Marissa Balmith, Mahmoud E S Soliman
The first account of the dynamic features of the loop region of VP40 of the Ebola virus was studied using accelerated molecular dynamics simulations and reported herein. Among the proteins of the Ebola virus, the matrix protein (VP40) plays a significant role in the virus lifecycle thereby making it a promising therapeutic target. Of interest is the newly elucidated N-terminal domain loop region of VP40 comprising residues K127, T129, and N130 which when mutated to alanine have demonstrated an unrecognized role for N-terminal domain-plasma membrane interaction for efficient VP40-plasma membrane localization, oligomerization, matrix assembly, and egress...
March 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28111710/ip-10-cxcr3-axis-promotes-the-proliferation-of-vascular-smooth-muscle-cells-through-erk1-2-creb-signaling-pathway
#10
Hui-Jin Wang, Yu Zhou, Rui-Ming Liu, Yuan-Sen Qin, Ying-Huan Cen, Ling-Yu Hu, Shen-Ming Wang, Zuo-Jun Hu
Excessive proliferation of vascular smooth muscle cells is one of the main pathological processes leading to atherosclerosis and intimal hyperplasia after vascular interventional therapy. Our previous study has shown that interferon-γ inducible protein-10 contributes to the proliferation of vascular smooth muscle cell. However, the underlying mechanisms remain unclear. Extracellular signal-regulated kinase 1/2, serine/threonine kinase Akt, and cAMP response element binding protein are signaling pathways, which are considered to play important roles in the processes of vascular smooth muscle cell proliferation...
March 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28101696/role-of-the-helix-in-talin-f3-domain-f3-helix-in-talin-mediated-integrin-activation
#11
Ang Li, Qiang Guo, Ailin Wei, Yaliang Zhou, Weiming Hu
Increases in ligand binding to cellular integrins (activation) play an important role in platelet and leukocyte function. Talin is necessary in vivo and sufficient in vitro for integrin αIIbβ3 activation. The precise mechanisms by which talin activates integrin are still being elucidated. In particular, talin undergoes conformational changes (around the F3 helix) and inserts the F3 helix into lipid bilayer; however, the connection between this lipid-inserting mechanism of talin and talin's capacity to activate integrin has never been explored before...
March 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28054303/amino-acid-properties-of-trafficking-determinants-in-the-outer-pore-forming-region-of-kv1-potassium-channels-in-cell-lines
#12
Barbara Gomez, Jing Zhu, Esperanza Recio-Pinto, William B Thornhill
Different classes of Kv1 potassium channels have different trafficking patterns despite having very similar amino acid sequences. Two amino acids responsible for these differences have been identified in the outer pore turret region of Kv1.1 and Kv1.4. Here we tested a series of substitutions at these two determinants on Kv1.4. All P506 substitutions tested resulted in a significant decrease in surface protein, total protein, and protein half-life, indicating that proline is required at 506 to stabilize protein conformation and increase trafficking to the cell surface...
March 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28025803/metabolic-transition-of-milk-lactose-synthesis-and-up-regulation-by-akt1-in-sows-from-late-pregnancy-to-lactation
#13
Fang Chen, Baoliang Chen, Wutai Guan, Jun Chen, Yantao Lv, Hanzhen Qiao, Chaoxian Wang, Yinzhi Zhang
Lactose plays a crucial role in controlling milk volume by inducing water toward into the mammary secretory vesicles from the mammary epithelial cell cytoplasm, thereby maintaining osmolality. In current study, we determined the expression of several lactose synthesis related genes, including glucose transporters (glucose transporter 1, glucose transporter 8, sodium-glucose cotransporter 1, sodium-glucose cotransporter 3, and sodium-glucose cotransporter 5), lactose synthases (α-lactalbumin and β1,4-galactosyltransferase), and hexokinases (hexokinase-1 and hexokinase-2) in sow mammary gland tissue at day 17 before delivery, on the 1st day of lactation and at peak lactation...
March 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27990613/site-specific-acetyl-lysine-antibodies-reveal-differential-regulation-of-histone-acetylation-upon-kinase-inhibition
#14
Shi Chen, Suping Chen, Qianqian Duan, Guoqiang Xu
Lysine acetylation regulates diverse biological functions for the modified proteins. Mass spectrometry-based proteomic approaches have identified thousands of lysine acetylation sites in cells and tissues. However, functional studies of these acetylation sites were limited by the lack of antibodies recognizing the specific modification sites. Here, we generated 55 site-specific acetyl lysine antibodies for the detection of this modification in cell lysates and evaluated the quality of these antibodies. Based on the immunoblotting analyses, we found that the nature of amino acid sequences adjacent to the modification sites affected the specificity of the site-specific acetyl lysine antibodies...
March 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27981422/effects-of-electroporation-on-tamoxifen-delivery-in-estrogen-receptor-positive-er-human-breast-carcinoma-cells
#15
Meric Arda Esmekaya, Handan Kayhan, Munci Yagci, Alaaddin Coskun, Ayse G Canseven
Estrogen receptor positive breast cancer is the most common type of breast cancer and in most cases, hormone therapy is considered complementary to surgery. Tamoxifen is one of the most common drugs used in hormone therapy for treating estrogen receptor positive breast cancer cells. However, it has severe side-effects depending on the duration of treating breast cancer and amount of tamoxifen used. In this study, we examined the effects of electroporation on the tamoxifen uptake in estrogen receptor positive MCF-7 breast cancer cells...
March 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27981421/identification-of-binding-mode-and-prospective-structural-features-of-novel-nef-protein-inhibitors-as-potential-anti-hiv-drugs
#16
Suri Moonsamy, Soumendranath Bhakat, Muthusamy Ramesh, Mahmoud E S Soliman
Human immunodeficiency virus (HIV)-negative factor (Nef) protein is an accessory pathogenic factor, which plays a significant role in acquired immune deficiency syndrome (AIDS). Nef deficient HIV virus took a longer time to progress into AIDS. Therefore, targeting Nef protein is considered as a key strategy towards HIV/AIDS treatment. Up-to-date, only few compounds were reported as Nef inhibitors. This has prompted us to provide a first account of an integrated computational framework in order to identify more potential Nef inhibitors...
March 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27933465/nano-mechanical-characterization-of-ataxia-telangiectasia-cells-treated-with-dexamethasone
#17
Michele Menotta, Sara Biagiotti, Giulia Bartolini, Bianchi Marzia, Sara Orazi, Aldo Germani, Luciana Chessa, Mauro Magnani
Ataxia telangiectasia is a rare genetic disease and no therapy is currently available. Glucocorticoid analogues have been shown to improve the neurological symptoms of treated patients. In the present study ataxia telangiectasia and wild type cells were used as a cellular model and treated with dexamethasone. The cells were subsequently investigated for membrane and whole cell mechanical properties by atomic force microscopy. In addition, cytoskeleton protein dynamics and nuclear shapes were assayed by fluorescence microscopy, while western blots were used to assess actin and tubulin content...
March 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27914004/hybrid-receptor-bound-mm-gbsa-per-residue-energy-based-pharmacophore-modelling-enhanced-approach-for-identification-of-selective-lta4h-inhibitors-as-potential-anti-inflammatory-drugs
#18
Patrick Appiah-Kubi, Mahmoud Soliman
Leukotriene A4 hydrolase has been identified as an enzyme with dual anti- and pro-inflammatory role, thus, the conversion of leukotriene to leukotriene B4 in the initiation stage of inflammation and the removal of the chemotactic Pro-Gly-Pro tripeptide. These findings make leukotriene A4 hydrolase an attractive drug target: suggesting an innovative approach towards the identification and design of novel class of compounds that can selectively inhibit leukotriene B4 synthesis while sparing the aminopeptidase activity...
March 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27914003/n-terminal-pro-b-type-natriuretic-peptide-levels-are-linked-with-modified-child-pugh-classification-in-patients-with-nonalcoholic-cirrhosis-nt-probnp-and-liver-cirrhosis
#19
Abdulbaki Kumbasar, Mehtap Navdar, Esra Ataoglu, Isil Uzunhasan, Kadir Ergen, Sule Poturoglu, Filiz Basinoglu, Fatih Yilmaz, Mustafa Yenigun, Fuat Sar, Ozgur Tanriverdi
Excess N-terminal pro-brain natriuretic peptide secretion has been linked to cirrhosis in previously studies. The relationship of plasma N-terminal pro-brain natriuretic peptide levels and cardiac dysfunction determined by echocardiography were investigated in patients with nonalcoholic cirrhosis and a control group of chronic hepatitis. This study was designed as a cross-sectional study. Thirty-two men and thirty-three women who gave informed consent who were followed-up for chronic liver failure were enrolled...
March 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27830366/maturation-of-adipocytes-is-suppressed-by-fluid-shear-stress
#20
Jongyun Choi, Sei Young Lee, Yeong-Min Yoo, Chi Hyun Kim
Preadipocytes are mechano-responsive cells and their differentiation to adipocytes may be regulated by various types of physical stimulation. Understanding the mechanism of differentiation, which increases the number of adipocytes and lipid accumulation is important in the study of obesity-related diseases. In this study, we investigated the effects of physical stimulation at different stages of adipogenic differentiation using physiological levels of fluid shear stress. Preadipocytes were treated with dexamethasone, 3-isobutyl-1-methylxanthine and insulin for 3 days (induction period) and incubated for additional 6 days for maturation...
March 2017: Cell Biochemistry and Biophysics
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