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Cell Biochemistry and Biophysics

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https://www.readbyqxmd.com/read/30259334/low-temperature-epr-spectroscopy-as-a-probe-free-technique-for-monitoring-oxidants-formed-in-tumor-cells-and-tissues-implications-in-drug-resistance-and-oxphos-targeted-therapies
#1
Balaraman Kalyanaraman, Gang Cheng, Jacek Zielonka, Brian Bennett
Oxidants formed from oxidative and nitrative metabolism include reactive oxygen species (ROS) such as superoxide, hydrogen peroxide/lipid hydroperoxides and reactive nitrogen species (RNS) (e.g., peroxynitrite [ONOO- ] and nitrogen dioxide), and reactive halogenated species (e.g., hypochlorous acid [HOCl]). Increasingly, ROS and RNS are implicated in tumorigenesis as well as tumor growth, progression, and metastasis. Recently, ROS were implicated in drug resistance, metabolic reprogramming, and T-cell metabolism in immunotherapy...
September 26, 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/30218405/superoxide-formation-in-cardiac-mitochondria-and-effect-of-phenolic-antioxidants
#2
Arina L Dudylina, Marina V Ivanova, Konstantin B Shumaev, Enno K Ruuge
Since mitochondria are the main cellular source of reactive oxygen species, it is important to study the effect of dietary phenolic compounds on the level of ROS in these organelles. Using the EPR spectroscopy and TIRON probe, the ability of the investigated phenols (quercetin, rutin, caffeic acid, curcumin, and resveratrol) to scavenge superoxide anion radicals generated by isolated heart mitochondria of Wistar rats under variable oxygen partial pressure was studied. It was shown that during a 10 min incubation, caffeic acid in concentrations of 10-500 μM most effectively scavenged superoxide radicals formed in the complex III of the mitochondrial respiratory chain...
September 14, 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/30191452/the-iron-state-in-spleen-and-liver-tissues-from-patients-with-hematological-malignancies-studied-using-magnetization-measurements-and-m%C3%A3-ssbauer-spectroscopy
#3
I V Alenkina, A V Vinogradov, I Felner, T S Konstantinova, E Kuzmann, V A Semionkin, M I Oshtrakh
In this overview, we present the results of the study of spleen and liver tissues taken from healthy donors in comparison with those from patients with (i) non-Hodgkin B-cell lymphomas, namely, mantle cell lymphoma and marginal zone B-cell lymphoma, (ii) acute myeloid leukemia, and (iii) primary myelofibrosis. The study was carried out using Mössbauer spectroscopy and magnetization measurements for the analysis of ferritin-like iron in spleen and liver tissues. Magnetization measurements demonstrated small differences in the saturation magnetic moments and revealed additional paramagnetic components...
September 7, 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/30343407/the-effects-of-resveratrol-metformin-cold-and-strength-training-on-the-level-of-perilipin-5-in-the-heart-skeletal-muscle-and-brown-adipose-tissues-in-mouse
#4
Fadaei Mehdi, Ghatreh Samani Keihan, Amini Seyed Asadollah, Farrokhi Effat
The high accumulation of lipid droplets in the cell is related to metabolic disorders, such as obesity. Perilipin 5 (Plin5), plays an important role in triglyceride hydrolysis in the lipid droplets. In this study, this protein has been evaluated in different tissues and conditions in mice. Fifty male mice were divided into 5 groups and treated for 45 days with Resveratrol, Metformin, strength training, and 4 °C cold. Brown adipose tissue (BAT), gastrocnemius skeletal muscle and heart were isolated for RNA extraction...
December 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/30324559/melatonin-modulates-lipid-metabolism-in-hepg2-cells-cultured-in-high-concentrations-of-oleic-acid-ampk-pathway-activation-may-play-an-important-role
#5
Yan Mi, Dehong Tan, Yin He, Xin Zhou, Qian Zhou, Shujuan Ji
Melatonin exists as an active ingredient in several foods and has been reported to inhibit fatty liver disease in animals; however, its molecular mechanisms are not well elucidated. Herein, we explored effects of melatonin on lipid accumulation induced by oleic acid in HepG2 cells and characterized the underlying molecular mechanisms. Pretreatment with melatonin (0.1-0.3 mM) significantly inhibited accumulation of triglyceride and cholesterol induced by incubating HepG2 cells with high concentrations of oleic acid (oleic acid overload) (p < 0...
December 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/30191451/can-macromolecular-nitroxide-work-as-extracellular-superoxide-dismutase-mimetic-in-cancer-and-stroke-therapy
#6
Carleton J C Hsia, W Keith Miskimins
No abstract text is available yet for this article.
December 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/30159781/calcium-induces-mitochondrial-oxidative-stress-because-of-its-binding-to-adenine-nucleotide-translocase
#7
Francisco Correa, Natalia Pavón, Mabel Buelna-Chontal, Natalia Chiquete-Félix, Luz Hernández-Esquivel, Edmundo Chávez
Several studies have demonstrated that the mitochondrial membrane switches from selective to non-selective permeability because of its improved matrix Ca2+ accumulation and oxidative stress. This process, known as permeability transition, evokes severe dysfunction in mitochondria through the opening of a non-specific pore, whose chemical nature is still under discussion. There are some proposals regarding the components of the pore structure, e.g., the adenine nucleotide translocase and dimers of the F1 Fo-ATP synthase...
December 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/30132185/biophysical-characterization-of-sg2na-variants-and-their-interaction-with-dj-1-and-calmodulin-in-vitro
#8
Sangeeta Soni, Buddhi Prakash Jain, Richa Gupta, Sudhaker Dharavath, Karunakar Kar, Sneha Sudha Komath, Shyamal K Goswami
SG2NA was first discovered as nuclear autoantigen in lung and bladder cancer patient. It was named SG2NA as its expression increases during S to G2 phase of cell cycle. SG2NA/Striatin3 was classified as a member of Striatin family along with Straitin and Zinedin due to its structural and functional relatedness. At the molecular level, SG2NA is characterized by the presence of multiple protein-protein interaction domains viz., a caveolin binding motif, a coiled coil structure, Ca2+ -calmodulin binding domain and a large WD-40 repeat domain in the same order from amino to the carboxyl termini...
December 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/30073572/induced-mutation-proves-a-potential-target-for-tb-therapy-a-molecular-dynamics-study-on-lprg
#9
Kgothatso E Machaba, Ndumiso N Mhlongo, Mahmoud E S Soliman
Molecular dynamics (MD) simulations of wild-type and V91W mutant Mycobacterium tuberculosis-LprG (Mtb-LprG) were performed with the goal to provide a comprehensive understanding of the Mtb-LprG as a potential antimycobacterial target. A long-range MD simulations and post-MD analyzes led us to various results that plainly explained the impact of V91W mutation on Mtb-LprG. Herein, the results revealed that the wild-type is less stable compared to V91W mutant. This was further supported by root mean square fluctuation, where the V91W mutant showed a higher degree of flexibility compared to the wild-type...
September 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/30062659/quantum-chemical-calculations-and-statistical-analysis-structural-cytotoxicity-relationships-of-some-synthesized-2-thiophen-naphtho-benzo-oxazinone-derivatives
#10
Mohammed B Alshammari, Mohammed H Geesi, El Hassane Anouar, Rashad Al-Salahi, Abdulrahman I Alharthi, Yasser Elnakady, Mohamed Marzouk
Twenty-two 2-thiophen-naphtho(benzo)oxazinone derivatives are prepared using 3-amino-2-naphthoic and 5-nitroanthranilic acids as building blocks. The target compounds (1-22) were evaluated quantitatively for their cytotoxic effects in vitro against three cancer cell lines, including the lung A549, the hepatocyte HepG2, and the breast MCF-7 carcinoma cells. Compounds 1, 12, 14, and 21 were found to exhibit remarkable cytotoxicity against the tested cancer cell lines. Compound 21 has shown the highest activity against A549 and MCF-7 (IC50 : 9...
September 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/30022374/endocytosis-of-g-protein-coupled-receptors-and-their-ligands-is-there-a-role-in-metal-trafficking
#11
REVIEW
Katerina Christofides, Resmi Menon, Christopher E Jones
The prevalence of metal dysregulation in many neurodegenerative and neurocognitive disorders has compelled many studying such diseases to investigate the mechanisms underlying metal regulation in the central nervous system. Metal homoeostasis is often complex, with sophisticated, multilayered pathways in operation. G protein-coupled receptors are omnipresent on cell membranes and have intriguing mechanisms of endocytosis and trafficking that may be useful in metal homoeostasis. Indeed, many receptors and/or their cognate ligands are able to bind metals, and in many cases metals are considered to have neuromodulatory roles as a result of receptor binding...
September 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29956081/p47phox-dependent-reactive-oxygen-species-stimulate-nuclear-translocation-of-the-foxo1-transcription-factor-during-metabolic-inhibition-in-cardiomyoblasts
#12
Ellis N Ter Horst, Nynke E Hahn, Dirk Geerts, René J P Musters, Walter J Paulus, Albert C van Rossum, Christof Meischl, Jan J Piek, Hans W M Niessen, Paul A J Krijnen
Reactive oxygen species (ROS) control forkhead box O (FOXO) transcription factor activity by influencing their nuclear translocation. However, knowledge of the ROS cellular source(s) involved herein remains scarce. Recently, we have shown p47phox -dependent activation of ROS-producing NADPH oxidase (NOX) at the nuclear pore in H9c2 rat cardiomyoblasts in response to ischemia. This localizes NOX perfectly to affect protein nuclear translocation, including that of transcription factors. In the current study, involvement of p47phox -dependent production of ROS in the nuclear translocation of FOXO1 was analyzed in H9c2 cells following 4 h of metabolic inhibition (MI), which mimics the effects of ischemia...
September 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29687225/hyphenated-3d-qsar-statistical-model-drug-repurposing-analysis-for-the-identification-of-potent-neuraminidase-inhibitor
#13
K Rohini, V Shanthi
The Influenza A virus is one of the principle causes of respiratory illness in human. The surface glycoprotein of the influenza virus, neuraminidase (NA), has a vital role in the release of new viral particle and spreads infection in the respiratory tract. It has been long recognized as a valid drug target for influenza A virus infection. Oseltamivir is used as a standard drug of choice for the treatment of influenza. However, the emergence of mutants with novel mutations has increased the resistance to potent NA inhibitor...
September 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29556953/correction-to-isolation-and-characterization-of-a-microrna-size-secretable-small-rna-in-streptococcus-sanguinis
#14
Ji-Woong Choi, Tae-Yub Kwon, Su-Hyung Hong, Heon-Jin Lee
The original version of this article unfortunately contains a mistake in the fund number under Acknowledgment section. The funding number should read as "2017R1A5A2015391 and 2015R1D1A3A01019684".
September 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29511990/on-the-regularities-of-the-polar-profiles-of-proteins-related-to-ebola-virus-infection-and-their-functional-domains
#15
Carlos Polanco, José Lino Samaniego Mendoza, Thomas Buhse, Vladimir N Uversky, Ingrid Paola Bañuelos Chao, Marcela Angola Bañuelos Cedano, Fernando Michel Tavera, Daniel Michel Tavera, Manuel Falconi, Abelardo Vela Ponce de León
The number of fatalities and economic losses caused by the Ebola virus infection across the planet culminated in the havoc that occurred between August and November 2014. However, little is known about the molecular protein profile of this devastating virus. This work represents a thorough bioinformatics analysis of the regularities of charge distribution (polar profiles) in two groups of proteins and their functional domains associated with Ebola virus disease: Ebola virus proteins and Human proteins interacting with Ebola virus...
September 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/30047043/conformational-changes-and-membrane-interaction-of-the-bacterial-phospholipase-exou-characterization-by-site-directed-spin-labeling
#16
Jimmy B Feix, Samantha Kohn, Maxx H Tessmer, David M Anderson, Dara W Frank
Numerous pathogenic bacteria produce proteins evolved to facilitate their survival and dissemination by modifying the host environment. These proteins, termed effectors, often play a significant role in determining the virulence of the infection. Consequently, bacterial effectors constitute an important class of targets for the development of novel antibiotics. ExoU is a potent phospholipase effector produced by the opportunistic pathogen Pseudomonas aeruginosa. Previous studies have established that the phospholipase activity of ExoU requires non-covalent interaction with ubiquitin, however the molecular details of the mechanism of activation and the manner in which ExoU associates with a target lipid bilayer are not understood...
July 25, 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/30003432/transforming-growth-factor-beta-tfg-%C3%AE-concentration-isoforms-are-diminished-in-acute-coronary-syndrome
#17
Jorge Ramón Padilla-Gutiérrez, Emmanuel Valdés-Alvarado, Saraí Citlalic Rodríguez-Reyes, Juan Arellano-Martin, Héctor Enrique Flores-Salinas, José Francisco Muñoz Valle, Yeminia Valle
Acute coronary syndrome (ACS) is the leading cause of death in elderly patients worldwide. Due its participation in apoptosis, fibrosis, and angiogenesis, transforming growth factor-β (TGF-β) isoforms had been categorized as risk factors for cardiovascular diseases. However, due their contradictory activities, a cardioprotective role has been suggested. The aim was to measure the plasma levels of TGF-β1, 2, and 3 proteins in patients with ACS. This was a case-control study including 225 subjects. The three activated isoforms were measured in serum using the Bio-Plex Pro TGF-β assay by means of magnetic beads; the fluorescence intensity of reporter signal was read in a Bio-Plex Magpix instrument...
July 12, 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29943362/uniform-field-resonators-for-epr-spectroscopy-a-review
#18
James S Hyde, Jason W Sidabras, Richard R Mett
Cavity resonators are often used for electron paramagnetic resonance (EPR). Rectangular TE102 and cylindrical TE011 are common modes at X-band even though the field varies cosinusoidally along the Z-axis. The authors found a way to create a uniform field (UF) in these modes. A length of waveguide at cut-off was introduced for the sample region, and tailored end sections were developed that supported the microwave resonant mode. This work is reviewed here. The radio frequency (RF) magnetic field in loop-gap resonators (LGR) at X-band is uniform along the Z-axis of the sample, which is a benefit of LGR technology...
June 25, 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29926429/targeting-outer-membrane-protein-component-adec-for-the-discovery-of-efflux-pump-inhibitor-against-adeabc-efflux-pump-of-multidrug-resistant-acinetobacter-baumannii
#19
Privita Verma, Vishvanath Tiwari
The structure and functioning of multidrug efflux systems provide us with a better understanding of the transport of various antibiotics, thus giving a path for the discovery of effective compounds for combating the multidrug resistance in Acinetobacter baumannii. In the present study, a number of computational techniques have been used to search for an inhibitor for the RND efflux pump, AdeABC, of A. baumannii targeting specifically its outermost component, i.e., AdeC. We have prepared the three-dimensional structure for AdeC using MODELLER v9...
June 20, 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29572698/2018-letter-from-the-editor-in-chief
#20
EDITORIAL
Lawrence Berliner
No abstract text is available yet for this article.
June 2018: Cell Biochemistry and Biophysics
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