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Cell Biochemistry and Biophysics

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https://www.readbyqxmd.com/read/29134494/expression-of-telomere-associated-proteins-is-interdependent-to-stabilize-native-telomere-structure-and-telomere-dysfunction-by-g-quadruplex-ligand-causes-terra-upregulation
#1
Ratan Sadhukhan, Priyanka Chowdhury, Sourav Ghosh, Utpal Ghosh
Telomere DNA can form specialized nucleoprotein structure with telomere-associated proteins to hide free DNA ends or G-quadruplex structures under certain conditions especially in presence of G-quadruplex ligand. Telomere DNA is transcribed to form non-coding telomere repeat-containing RNA (TERRA) whose biogenesis and function is poorly understood. Our aim was to find the role of telomere-associated proteins and telomere structures in TERRA transcription. We silenced four [two shelterin (TRF1, TRF2) and two non-shelterin (PARP-1, SLX4)] telomere-associated genes using siRNA and verified depletion in protein level...
November 13, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29116599/the-class-a-gpcr-dopamine-d2-receptor-forms-transient-dimers-stabilized-by-agonists-detection-by-single-molecule-tracking
#2
Rinshi S Kasai, Shuichi V Ito, Ryo M Awane, Takahiro K Fujiwara, Akihiro Kusumi
Whether class-A G-protein coupled receptors (GPCRs) exist and work as monomers or dimers has drawn extensive attention. A class-A GPCR dopamine D2 receptor (D2R) is involved in many physiological and pathological processes and diseases, indicating its critical role in proper functioning of neuronal circuits. In particular, D2R homodimers might play key roles in schizophrenia development and amphetamine-induced psychosis. Here, using single-molecule imaging, we directly tracked single D2R molecules in the plasma membrane at a physiological temperature of 37 °C, and unequivocally determined that D2R forms transient dimers with a lifetime of 68 ms in its resting state...
November 7, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29098642/oxidation-induced-increase-in-photoreactivity-of-bovine-retinal-lipid-extract
#3
A Koscielniak, M Serafin, M Duda, T Oles, A Zadlo, A Broniec, O Berdeaux, S Gregoire, L Bretillon, T Sarna, A Pawlak
The mammalian retina contains a high level of polyunsaturated fatty acids, including docosahexaenoic acid (22:6) (DHA), which are highly susceptible to oxidation. It has been shown that one of the products of DHA oxidation-carboxyethylpyrrole (CEP), generated in situ, causes modifications of retinal proteins and induces inflammation response in the outer retina. These contributing factors may play a role in the development of age-related macular degeneration (AMD). It is also possible that some of the lipid oxidation products are photoreactive, and upon irradiation with blue light may generate reactive oxygen species...
November 2, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29090414/liver-genes-expression-induced-by-tamoxifen-loaded-solid-lipid-nanoparticles-in-wistar-female-rats
#4
Naser Zanganeh, Nasrin Ziamajidi, Iraj Khodadadi, Massoud Saidijam, Roghayeh Abbasalipourkabir
The objective of this study was to investigate the effect of free tamoxifen and tamoxifen-loaded solid lipid nanoparticles (SLN) on cytochrome P450 (CYP3A2) and flavin-containing monooxygenase1 (FMO1) genes expression in the liver of female Wistar rats. Thirty female Wistar rats aged 7-8 weeks, were divided into six groups of six rats each. The first, second, third, and fourth groups were ovariectomized and received tamoxifen (2  mg/kg of body weight dissolved in 1  ml olive oil), tamoxifen-loaded SLN (2  mg/kg of body weight dispersed in 1  ml olive oil), SLN (10  mg/kg of body weight dispersed in 1  ml olive oil), and 1  ml olive oil, respectively...
October 31, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29071513/xth-epr-workshop-on-applications-of-epr-in-biology-and-medicine
#5
EDITORIAL
Tadeusz Sarna, Balaraman Kalyanaraman, Lawrence Berliner
No abstract text is available yet for this article.
October 25, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29067585/intrinsic-response-towards-physiologic-stiffness-is-cell-type-dependent
#6
Michael Reimer, Silviya Petrova Zustiak, Saahil Sheth, Joseph Martin Schober
In the continuous search for better tissue engineering scaffolds it has become increasingly clear that the substrate properties dramatically affect cell responses. Here we compared cells from a physiologically stiff tissue, melanoma, to cells isolated from a physiologically soft tissue, brain. We measured the cell line responses to laminin immobilized onto glass or polyacrylamide hydrogels tuned to have a Young's modulus ranging from 1 to 390 kPa. Single cells were analyzed for spreading area, shape, total actin content, actin-based morphological features and modification of immobilized laminin...
October 24, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29039057/the-effects-of-low-doses-of-gamma-radiation-on-growth-and-membrane-activity-of-pseudomonas-aeruginosa-grp3-and-escherichia-coli-m17
#7
D Soghomonyan, A Margaryan, K Trchounian, K Ohanyan, H Badalyan, A Trchounian
Microorganisms are part of the natural environments and reflect the effects of different physical factors of surrounding environment, such as gamma (γ) radiation. This work was devoted to the study of the influence of low doses of γ radiation with the intensity of 2.56 μW (m(2) s)(-1) (absorbed doses were 3.8 mGy for the radiation of 15 min and 7.2 mGy-for 30 min) on Escherichia coli M-17 and Pseudomonas aeruginosa GRP3 wild type cells. The changes of bacterial, growth, survival, morphology, and membrane activity had been studied after γ irradiation...
October 16, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28986856/spin-lattice-relaxation-epr-po2-images-may-direct-the-location-of-radiation-tumor-boosts-to-enhance-tumor-cure
#8
Boris Epel, Martyna Krzykawska-Serda, Victor Tormyshev, Matthew C Maggio, Eugene D Barth, Charles A Pelizzari, Howard J Halpern
Radiation treatment success and high tumor oxygenation and success have been known to be highly correlated. This suggests that radiation therapy guided by images of tumor regions with low oxygenation, oxygen-guided radiation therapy (OGRT) may be a promising enhancement of cancer radiation treatment. Before applying the technique to human subjects, OGRT needs to be tested in animals, most easily in rodents. Electron paramagnetic resonance imaging provides quantitative maps of tissue and tumor oxygen in rodents with 1 mm spatial resolution and 1 torr pO2 resolution at low oxygen levels...
October 6, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28975596/erratum-to-hypoxia-augments-increased-hif-1a-and-reduced-survival-protein-p-akt-in-gelsolin-gsn-dependent-cardiomyoblast-cell-apoptosis
#9
Yu-Lan Yeh, Wei-Syun Hu, Wei-Jen Ting, Chia-Yao Shen, Hsi-Hsien Hsu, Li-Chin Chung, Chuan-Chou Tu, Sheng-Huang Chang, Cecilia-Hsuan Day, Yuhsin Tsai, Chih-Yang Huang
No abstract text is available yet for this article.
October 3, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28952073/cysteine-to-serine-conversion-at-111th-position-renders-the-disaggregation-and-retains-the-stabilization-of-detrimental-sod1-a4v-mutant-against-amyotrophic-lateral-sclerosis-in-human-a-discrete-molecular-dynamics-study
#10
E Srinivasan, R Rajasekaran
Protein aggregation is a hallmark of various neurodegenerative disorders, such as amyotrophic lateral sclerosis (ALS) in humans. Mutations in Cu/Zn superoxide dismutase (SOD1) protein were found to be a prominent cause behind the majority of the familial ALS cases with abnormal protein aggregates. Herein, we report the biophysical characterization of the beneficial mutation C111S that stabilizes the SOD1 harboring A4V mutation, one of the most lethal diseases causing mutant that leads to protein destabilization and aggregation...
September 26, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28948569/blood-free-radicals-concentration-determined-by-electron-paramagnetic-resonance-spectroscopy-and-delayed-cerebral-ischemia-occurrence-in-patients-with-aneurysmal-subarachnoid-hemorrhage
#11
Grzywna Ewelina, Stachura Krzysztof, Moskala Marek, Kruczala Krzysztof
Pathophysiology of delayed cerebral ischemia and cerebral vasospasm following aneurysmal subarachnoid hemorrhage is still poorly recognized, however free radicals are postulated as one of the crucial players. This study was designed to scrutinize whether the concentration of free radicals in the peripheral venous blood is related to the occurrence of delayed cerebral ischemia associated with cerebral vasospasm. Twenty-four aneurysmal subarachnoid hemorrhage patients and seven patients with unruptured intracranial aneurysm (control group) have been studied...
September 25, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28942575/a-contact-based-method-for-differentiation-of-human-mesenchymal-stem-cells-into-an-endothelial-cell-phenotype
#12
Binata Joddar, Shweta Anil Kumar, Alok Kumar
Adult stem cells such as mesenchymal stem cells (MSC) are known to possess the ability to augment neovascularization processes and are thus widely popular as an autologous source of progenitor cells. However there is a huge gap in our current knowledge of mechanisms involved in differentiating MSC into endothelial cells (EC), essential for lining engineered blood vessels. To fill up this gap, we attempted to differentiate human MSC into EC, by culturing the former onto chemically fixed layers of EC or its ECM, respectively...
September 23, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28660426/recent-developments-in-the-probes-and-assays-for-measurement-of-the-activity-of-nadph-oxidases
#13
Jacek Zielonka, Micael Hardy, Radosław Michalski, Adam Sikora, Monika Zielonka, Gang Cheng, Olivier Ouari, Radosław Podsiadły, Balaraman Kalyanaraman
NADPH oxidases are a family of enzymes capable of transferring electrons from NADPH to molecular oxygen. A major function of NADPH oxidases is the activation of molecular oxygen into reactive oxygen species. Increased activity of NADPH oxidases has been implicated in various pathologies, including cardiovascular disease, neurological dysfunction, and cancer. Thus, NADPH oxidases have been identified as a viable target for the development of novel therapeutics exhibiting inhibitory effects on NADPH oxidases...
December 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28555359/broadband-w-band-rapid-frequency-sweep-considerations-for-fourier-transform-epr
#14
Robert A Strangeway, James S Hyde, Theodore G Camenisch, Jason W Sidabras, Richard R Mett, James R Anderson, Joseph J Ratke, Witold K Subczynski
A multi-arm W-band (94 GHz) electron paramagnetic resonance spectrometer that incorporates a loop-gap resonator with high bandwidth is described. A goal of the instrumental development is detection of free induction decay following rapid sweep of the microwave frequency across the spectrum of a nitroxide radical at physiological temperature, which is expected to lead to a capability for Fourier transform electron paramagnetic resonance. Progress toward this goal is a theme of the paper. Because of the low Q-value of the loop-gap resonator, it was found necessary to develop a new type of automatic frequency control, which is described in an appendix...
December 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28434138/dynamic-epr-oximetry-of-changes-in-intracerebral-oxygen-tension-during-induced-thromboembolism
#15
Huagang Hou, Nadeem Khan, Sangeeta Gohain, Clifford J Eskey, Karen L Moodie, Kirk J Maurer, Harold M Swartz, Periannan Kuppusamy
Cerebral tissue oxygenation (oxygen tension, pO2) is a critical parameter that is closely linked to brain metabolism, function, and pathophysiology. In this work, we have used electron paramagnetic resonance oximetry with a deep-tissue multi-site oxygen-sensing probe, called implantable resonator, to monitor temporal changes in cerebral pO2 simultaneously at four sites in a rabbit model of ischemic stroke induced by embolic clot. The pO2 values in healthy brain were not significantly different among the four sites measured over a period of 4 weeks...
December 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28434137/cholesterol-hydroperoxide-generation-translocation-and-reductive-turnover-in-biological-systems
#16
Albert W Girotti, Witold Korytowski
Cholesterol is like other unsaturated lipids in being susceptible to peroxidative degradation upon exposure to strong oxidants like hydroxyl radical or peroxynitrite generated under conditions of oxidative stress. In the eukaryotic cell plasma membrane, where most of the cellular cholesterol resides, peroxidation leads to membrane structural and functional damage from which pathological states may arise. In low density lipoprotein, cholesterol and phospholipid peroxidation have long been associated with atherogenesis...
December 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28429253/modified-metformin-as-a-more-potent-anticancer-drug-mitochondrial-inhibition-redox-signaling-antiproliferative-effects-and-future-epr-studies
#17
Balaraman Kalyanaraman, Gang Cheng, Micael Hardy, Olivier Ouari, Adam Sikora, Jacek Zielonka, Michael B Dwinell
Metformin, one of the most widely prescribed antidiabetic drugs in the world, is being repurposed as a potential drug in cancer treatment. Epidemiological studies suggest that metformin exerts anticancer effects in diabetic patients with pancreatic cancer. However, at typical antidiabetic doses the bioavailability of metformin is presumably too low to exert antitumor effects. Thus, more potent analogs of metformin are needed in order to increase its anticancer efficacy. To this end, a new class of mitochondria-targeted metformin analogs (or mito-metformins) containing a positively-charged lipophilic triphenylphosphonium group was synthesized and tested for their antitumor efficacy in pancreatic cancer cells...
December 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28417231/high-cholesterol-low-cholesterol-effects-in-biological-membranes-a-review
#18
Witold K Subczynski, Marta Pasenkiewicz-Gierula, Justyna Widomska, Laxman Mainali, Marija Raguz
Lipid composition determines membrane properties, and cholesterol plays a major role in this determination as it regulates membrane fluidity and permeability, as well as induces the formation of coexisting phases and domains in the membrane. Biological membranes display a very diverse lipid composition, the lateral organization of which plays a crucial role in regulating a variety of membrane functions. We hypothesize that, during biological evolution, membranes with a particular cholesterol content were selected to perform certain functions in the cells of eukaryotic organisms...
December 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28871484/epr-oximetry-sensor-developing-a-tam-derivative-for-in-vivo-studies
#19
Agnieszka Boś-Liedke, Magdalena Walawender, Anna Woźniak, Dorota Flak, Jacek Gapiński, Stefan Jurga, Małgorzata Kucińska, Adam Plewiński, Marek Murias, Marwa Elewa, Lisa Lampp, Peter Imming, Krzysztof Tadyszak
Oxygenation is one of the most important physiological parameters of biological systems. Low oxygen concentration (hypoxia) is associated with various pathophysiological processes in different organs. Hypoxia is of special importance in tumor therapy, causing poor response to treatment. Triaryl methyl (TAM) derivative radicals are commonly used in electron paramagnetic resonance (EPR) as sensors for quantitative spatial tissue oxygen mapping. They are also known as magnetic resonance imaging (MRI) contrast agents and fluorescence imaging compounds...
September 4, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28852971/identification-of-phytochemicals-targeting-c-met-kinase-domain-using-consensus-docking-and-molecular-dynamics-simulation-studies
#20
Shima Aliebrahimi, Shideh Montasser Kouhsari, Seyed Nasser Ostad, Seyed Shahriar Arab, Leila Karami
c-Met receptor tyrosine kinase is a proto-oncogene whose aberrant activation is attributed to a lower rate of survival in most cancers. Natural product-derived inhibitors known as "fourth generation inhibitors" constitute more than 60% of anticancer drugs. Furthermore, consensus docking approach has recently been introduced to augment docking accuracy and reduce false positives during a virtual screening. In order to obtain novel small-molecule Met inhibitors, consensus docking approach was performed using Autodock Vina and Autodock 4...
August 29, 2017: Cell Biochemistry and Biophysics
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