journal
MENU ▼
Read by QxMD icon Read
search

Cell Biochemistry and Biophysics

journal
https://www.readbyqxmd.com/read/29761345/mutational-analysis-of-the-cysteine-rich-region-of-the-iron-responsive-gata-factor-fep1-role-of-individual-cysteines-as-2fe-2s-cluster-ligands
#1
Maria Carmela Bonaccorsi di Patti, Antimo Cutone, Giovanni Musci
Fep1, the iron-dependent GATA-type transcriptional repressor of the methylotrophic yeast Pichia pastoris, has a dimeric structure and binds an iron-sulfur cluster of the [2Fe-2S] type. In this work, we extend the characterization of this protein by analysis of the optical and CD spectroscopic properties of a set of mutants where cysteines within the conserved Cys-X5 -Cys-X8- Cys-X2 -Cys motif have been targeted, in order to evaluate their role as [2Fe-2S] ligands. The results suggest that all four cysteine residues are essential because replacing them with serines in different combinations invariably produces a protein unable to correctly bind the [2Fe-2S] cluster...
May 14, 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29704106/applications-of-m%C3%A3-ssbauer-spectroscopy-in-biomedical-research
#2
M I Oshtrakh
A brief review on the applications of Mössbauer spectroscopy in biomedical research discusses the results of more than fifty years of experience in this field. Basing on the numerous results the main directions of biomedical applications of Mössbauer spectroscopy are considered as follows: 1) studies of the quantitative changes of iron-containing biomolecules related to pathological processes; 2) studies of the qualitative changes in iron-containing biomolecules related to pathological processes; 3) studies of the effect of various environmental factors (physical, chemical, and biological) on iron-containing biomolecules; 4) studies of metabolic processes by means of analysis of the Mössbauer nuclides pathways in organisms; 5) studies of dynamic processes; 6) studies of pharmaceutical compounds and blood substitutes containing Mössbauer nuclides; 7) miscellaneous studies...
April 27, 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29687225/hyphenated-3d-qsar-statistical-model-drug-repurposing-analysis-for-the-identification-of-potent-neuraminidase-inhibitor
#3
K Rohini, V Shanthi
The Influenza A virus is one of the principle causes of respiratory illness in human. The surface glycoprotein of the influenza virus, neuraminidase (NA), has a vital role in the release of new viral particle and spreads infection in the respiratory tract. It has been long recognized as a valid drug target for influenza A virus infection. Oseltamivir is used as a standard drug of choice for the treatment of influenza. However, the emergence of mutants with novel mutations has increased the resistance to potent NA inhibitor...
April 23, 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29572698/2018-letter-from-the-editor-in-chief
#4
EDITORIAL
Lawrence Berliner
No abstract text is available yet for this article.
March 23, 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29556953/correction-to-isolation-and-characterization-of-a-microrna-size-secretable-small-rna-in-streptococcus-sanguinis
#5
Ji-Woong Choi, Tae-Yub Kwon, Su-Hyung Hong, Heon-Jin Lee
The original version of this article unfortunately contains a mistake in the fund number under Acknowledgment section. The funding number should read as "2017R1A5A2015391 and 2015R1D1A3A01019684".
March 20, 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29511990/on-the-regularities-of-the-polar-profiles-of-proteins-related-to-ebola-virus-infection-and-their-functional-domains
#6
Carlos Polanco, José Lino Samaniego Mendoza, Thomas Buhse, Vladimir N Uversky, Ingrid Paola Bañuelos Chao, Marcela Angola Bañuelos Cedano, Fernando Michel Tavera, Daniel Michel Tavera, Manuel Falconi, Abelardo Vela Ponce de León
The number of fatalities and economic losses caused by the Ebola virus infection across the planet culminated in the havoc that occurred between August and November 2014. However, little is known about the molecular protein profile of this devastating virus. This work represents a thorough bioinformatics analysis of the regularities of charge distribution (polar profiles) in two groups of proteins and their functional domains associated with Ebola virus disease: Ebola virus proteins and Human proteins interacting with Ebola virus...
March 6, 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29380245/screening-of-potential-inhibitor-against-coat-protein-of-apple-chlorotic-leaf-spot-virus
#7
Rituraj Purohit, Sachin Kumar, Vipin Hallan
In this study, we analyzed Coat protein (CP) of Apple chlorotic leaf spot virus (ACLSV), an important latent virus on Apple. Incidence of the virus is upto 60% in various apple cultivars, affecting yield losses of the order of 10-40% (depending upon the cultivar). CP plays an important role as the sole building block of the viral capsid. Homology approach was used to model 193 amino acid sequence of the coat protein. We used various servers such as ConSurf, TargetS, OSML, COACH, COFACTOR for the prediction of active site residues in coat protein...
June 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29322360/highly-conserved-arg-residue-of-erfnin-motif-of-pro-domain-is-important-for-ph-induced-zymogen-activation-process-in-cysteine-cathepsins-k-and-l
#8
Pulakesh Aich, Sampa Biswas
Pro-domain of a cysteine cathepsin contains a highly conserved Ex2 Rx2 Fx2 Nx3 Ix3 N (ERFNIN) motif. The zymogen structure of cathepsins revealed that the Arg(R) residue of the motif is a central residue of a salt-bridge/H-bond network, stabilizing the scaffold of the pro-domain. Importance of the arginine is also demonstrated in studies where a single mutation (Arg → Trp) in human lysosomal cathepsin K (hCTSK) is linked to a bone-related genetic disorder "Pycnodysostosis". In the present study, we have characterized in vitro Arg → Trp mutant of hCTSK and the same mutant of hCTSL...
January 10, 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29313175/a-biomolecular-network-driven-proteinic-interaction-in-hcv-clearance
#9
Pratichi Singh, Febin Prabhu Dass J
Hepatitis C virus infection causes chronic liver disease that leads to cancer-related mortality. Presently around 30% of the HCV (infected) affected population get rid of the infection through spontaneous disease clearance. This phenomenon is conducted by a set of reported immune candidate genes. Hence, this study focuses only on these immune-response related genes with aid of network approach, where the idea is to disseminate the network for better understanding of key functional genes and their transcription control activity...
January 8, 2018: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29098642/oxidation-induced-increase-in-photoreactivity-of-bovine-retinal-lipid-extract
#10
A Koscielniak, M Serafin, M Duda, T Oles, A Zadlo, A Broniec, O Berdeaux, S Gregoire, L Bretillon, T Sarna, A Pawlak
The mammalian retina contains a high level of polyunsaturated fatty acids, including docosahexaenoic acid (22:6) (DHA), which are highly susceptible to oxidation. It has been shown that one of the products of DHA oxidation-carboxyethylpyrrole (CEP), generated in situ, causes modifications of retinal proteins and induces inflammation response in the outer retina. These contributing factors may play a role in the development of age-related macular degeneration (AMD). It is also possible that some of the lipid oxidation products are photoreactive, and upon irradiation with blue light may generate reactive oxygen species...
December 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29071513/xth-epr-workshop-on-applications-of-epr-in-biology-and-medicine
#11
EDITORIAL
Tadeusz Sarna, Balaraman Kalyanaraman, Lawrence Berliner
No abstract text is available yet for this article.
December 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28986856/spin-lattice-relaxation-epr-po-2-images-may-direct-the-location-of-radiation-tumor-boosts-to-enhance-tumor-cure
#12
Boris Epel, Martyna Krzykawska-Serda, Victor Tormyshev, Matthew C Maggio, Eugene D Barth, Charles A Pelizzari, Howard J Halpern
Radiation treatment success and high tumor oxygenation and success have been known to be highly correlated. This suggests that radiation therapy guided by images of tumor regions with low oxygenation, oxygen-guided radiation therapy (OGRT) may be a promising enhancement of cancer radiation treatment. Before applying the technique to human subjects, OGRT needs to be tested in animals, most easily in rodents. Electron paramagnetic resonance imaging provides quantitative maps of tissue and tumor oxygen in rodents with 1 mm spatial resolution and 1 torr pO2 resolution at low oxygen levels...
December 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28948569/blood-free-radicals-concentration-determined-by-electron-paramagnetic-resonance-spectroscopy-and-delayed-cerebral-ischemia-occurrence-in-patients-with-aneurysmal-subarachnoid-hemorrhage
#13
Grzywna Ewelina, Stachura Krzysztof, Moskala Marek, Kruczala Krzysztof
Pathophysiology of delayed cerebral ischemia and cerebral vasospasm following aneurysmal subarachnoid hemorrhage is still poorly recognized, however free radicals are postulated as one of the crucial players. This study was designed to scrutinize whether the concentration of free radicals in the peripheral venous blood is related to the occurrence of delayed cerebral ischemia associated with cerebral vasospasm. Twenty-four aneurysmal subarachnoid hemorrhage patients and seven patients with unruptured intracranial aneurysm (control group) have been studied...
December 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28849322/epr-technology-as-sensitive-method-for-oxidative-stress-detection-in-primary-and-secondary-keratinocytes-induced-by-two-selected-nanoparticles
#14
S B Lohan, S Ahlberg, A Mensch, D Höppe, M Giulbudagian, M Calderón, S Grether-Beck, J Krutmann, J Lademann, M C Meinke
Exogenous factors can cause an imbalance in the redox state of biological systems, promoting the development of oxidative stress, especially reactive oxygen species (ROS). To monitor the intensity of ROS production in secondary keratinocytes (HaCaT) by diesel exhaust particles and thermoresponsive nanogels (tNG), electron paramagnetic resonance (EPR) spectroscopy after 1 and 24 h of incubation, respectively, was applied. Their cytotoxicity was analyzed by a cell viability assay (XTT). For tNG an increase in the cell viability and ROS production of 10% was visible after 24 h, whereas 1 h showed no effect...
December 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28756482/epr-oximetry-of-cetuximab-treated-head-and-neck-tumours-in-a-mouse-model
#15
H Gustafsson, A Kale, A Dasu, A Lund, P-H Edqvist, K Roberg
Head and neck squamous cell carcinoma (HNSCC) tumours are associated with high mortality despite advances in therapy. The monoclonal antibody cetuximab (Erbitux® ) has been approved for the treatment of advanced HNSCC. However, only a subset of HNSC patients receiving cetuximab actually responds to treatment, underlining the need for a means to tailor treatments of individual patients. The aim of the present study was to investigate the effect of cetuximab treatment on tumour growth, on tumour partial oxygen pressure as measured by LiPc electron paramagnetic resonance oximetry and on the expression of proteins involved in tumour growth, metabolism and hypoxia...
December 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28687906/development-of-the-implantable-resonator-system-for-clinical-epr-oximetry
#16
Rose M Caston, Wilson Schreiber, Huagang Hou, Benjamin B Williams, Eunice Y Chen, Philip E Schaner, Lesley A Jarvis, Ann Barry Flood, Sergey V Petryakov, Maciej M Kmiec, Periannan Kuppusamy, Harold M Swartz
Hypoxic tumors are more resistant to radiotherapy and chemotherapy, which decreases the efficacy of these common forms of treatment. We have been developing implantable paramagnetic particulates to measure oxygen in vivo using electron paramagnetic resonance. Once implanted, oxygen can be measured repeatedly and non-invasively in superficial tissues (<3 cm deep), using an electron paramagnetic resonance spectrometer and an external surface-loop resonator. To significantly extend the clinical applications of electron paramagnetic resonance oximetry, we developed an implantable resonator system to obtain measurements at deeper sites...
December 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28660427/cholesterol-bilayer-domains-in-the-eye-lens-health-a-review
#17
REVIEW
Justyna Widomska, Witold K Subczynski, Laxman Mainali, Marija Raguz
The most unique biochemical characteristic of the eye lens fiber cell plasma membrane is its extremely high cholesterol content, the need for which is still unclear. It is evident, however, that the disturbance of Chol homeostasis may result in damages associated with cataracts. Electron paramagnetic resonance methods allow discrimination of two types of lipid domains in model membranes overloaded with Chol, namely, phospholipid-cholesterol domains and pure Chol bilayer domains. These domains are also detected in human lens lipid membranes prepared from the total lipids extracted from lens cortices and nuclei of donors from different age groups...
December 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28660426/recent-developments-in-the-probes-and-assays-for-measurement-of-the-activity-of-nadph-oxidases
#18
Jacek Zielonka, Micael Hardy, Radosław Michalski, Adam Sikora, Monika Zielonka, Gang Cheng, Olivier Ouari, Radosław Podsiadły, Balaraman Kalyanaraman
NADPH oxidases are a family of enzymes capable of transferring electrons from NADPH to molecular oxygen. A major function of NADPH oxidases is the activation of molecular oxygen into reactive oxygen species. Increased activity of NADPH oxidases has been implicated in various pathologies, including cardiovascular disease, neurological dysfunction, and cancer. Thus, NADPH oxidases have been identified as a viable target for the development of novel therapeutics exhibiting inhibitory effects on NADPH oxidases...
December 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28646414/dynamic-meso-scale-anchorage-of-gpi-anchored-receptors-in-the-plasma-membrane-prion-protein-vs-thy1
#19
Yuri L Nemoto, Roger J Morris, Hiroko Hijikata, Taka A Tsunoyama, Akihiro C E Shibata, Rinshi S Kasai, Akihiro Kusumi, Takahiro K Fujiwara
The central mechanism for the transmission of the prion protein misfolding is the structural conversion of the normal cellular prion protein to the pathogenic misfolded prion protein, by the interaction with misfolded prion protein. This process might be enhanced due to the homo-dimerization/oligomerization of normal prion protein. However, the behaviors of normal prion protein in the plasma membrane have remained largely unknown. Here, using single fluorescent-molecule imaging, we found that both prion protein and Thy1, a control glycosylphosphatidylinositol-anchored protein, exhibited very similar intermittent transient immobilizations lasting for a few seconds within an area of 24...
December 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28555359/broadband-w-band-rapid-frequency-sweep-considerations-for-fourier-transform-epr
#20
Robert A Strangeway, James S Hyde, Theodore G Camenisch, Jason W Sidabras, Richard R Mett, James R Anderson, Joseph J Ratke, Witold K Subczynski
A multi-arm W-band (94 GHz) electron paramagnetic resonance spectrometer that incorporates a loop-gap resonator with high bandwidth is described. A goal of the instrumental development is detection of free induction decay following rapid sweep of the microwave frequency across the spectrum of a nitroxide radical at physiological temperature, which is expected to lead to a capability for Fourier transform electron paramagnetic resonance. Progress toward this goal is a theme of the paper. Because of the low Q-value of the loop-gap resonator, it was found necessary to develop a new type of automatic frequency control, which is described in an appendix...
December 2017: Cell Biochemistry and Biophysics
journal
journal
32678
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"