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Journal of Biological Inorganic Chemistry: JBIC

Rafal Dutkiewicz, Malgorzata Nowak
Iron-sulfur (FeS) clusters are prosthetic groups critical for the function of many proteins in all domains of life. FeS proteins function in processes ranging from oxidative phosphorylation and cofactor biosyntheses to DNA/RNA metabolism and regulation of gene expression. In eukaryotic cells, mitochondria play a central role in the process of FeS biogenesis and support maturation of FeS proteins localized within mitochondria and in other cellular compartments. In humans, defects in mitochondrial FeS cluster biogenesis lead to numerous pathologies, which are often fatal...
November 9, 2017: Journal of Biological Inorganic Chemistry: JBIC
Monireh Dehkhodaei, Mehdi Sahihi, Hadi Amiri Rudbari, Fariborz Momenbeik
The interaction of three complexes [Zn(II), Cu(II), and V(IV)] derived from an asymmetric bidentate Schiff-base ligand with DNA and HSA was studied using fluorescence quenching, UV-Vis spectroscopy, viscosity measurements, and computational methods [molecular docking and our Own N-layered Integrated molecular Orbital and molecular Mechanics (ONIOM)]. The obtained results revealed that the DNA and HSA affinities for binding of the synthesized compounds follow as V(IV) > Zn(II) > Cu(II) and Zn(II) > V(IV) > Cu(II), respectively...
November 8, 2017: Journal of Biological Inorganic Chemistry: JBIC
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September 21, 2017: Journal of Biological Inorganic Chemistry: JBIC
Yan Du, Yubo Qin, Zizhen Li, Xiuying Yang, Jingchang Zhang, Harrison Westwick, Eve Tsai, Xudong Cao
A multifunctional nanobiomaterial has been developed by deliberately combining functions of superparamagnetism, fluorescence, and axonal tracing into one material. Superparamagnetic iron oxide nanoparticles were first synthesized and coated with a silica layer to prevent emission quenching through core-dye interactions; a fluorescent molecule, fluorescein isothiocyanate, was doped inside second layer of silica shell to improve photo-stability and to enable further thiol functionalization. Subsequently, biotinylated dextran amine, a sensitive axonal tracing reagent, was immobilized on the thiol-functionalized nanoparticle surfaces...
December 2017: Journal of Biological Inorganic Chemistry: JBIC
Jason Hon, Michelle S Hwang, Meara A Charnetzki, Issra J Rashed, Patrick B Brady, Sarah Quillin, Marvin W Makinen
Protein tyrosine phosphatases (PTPases) are a prominent focus of drug design studies because of their roles in homeostasis and disorders of metabolism. These studies have met with little success because (1) virtually all inhibitors hitherto exhibit only competitive behavior and (2) a consensus sequence H/V-C-X5-R-S/T characterizes the active sites of PTPases, leading to low specificity of active site directed inhibitors. With protein tyrosine phosphatase-1B (PTP1B) identifed as the target enzyme of the vanadyl (VO(2+)) chelate bis(acetylacetonato)oxidovanadium(IV) [VO(acac)2] in 3T3-L1 adipocytes [Ou et al...
December 2017: Journal of Biological Inorganic Chemistry: JBIC
Kaushik Banerjee, Manas Kumar Biswas, Soumitra Kumar Choudhuri
Induction of undesired toxicity and emergence of multidrug resistance (MDR) are the major obstacles for cancer treatment. Moreover, aggressive cancers are less sensitive towards existing chemotherapeutics. Therefore, selective targeting of cancers without inducing undesired side effects and designing proper strategies to overcome MDR has utmost importance in modern chemotherapy. Previously we revealed the anticancer properties of some transition metal chelates of Schiff base, but the effectiveness of nickel complex is still unrevealed...
December 2017: Journal of Biological Inorganic Chemistry: JBIC
Derek B Rice, Gayan B Wijeratne, Timothy A Jackson
Mn K-edge X-ray absorption spectroscopy experiments were performed on the solid- and solution-phase samples of [Mn(II)(dpaq(R))](OTf) (R=H, Me) and [Mn(III)(OH)(dpaq(R))](OTf). The extended X-ray absorption fine structure (EXAFS) data show distinct differences between the Mn(II) and Mn(III)-OH complexes, with fits providing metric parameters in excellent agreement with values from X-ray crystallography and density functional theory (DFT) computations. Evaluation of the EXAFS data for solid-phase [Mn(III)(OH)(dpaq)](OTf) resolved a short Mn-OH bond distance of 1...
December 2017: Journal of Biological Inorganic Chemistry: JBIC
Halit Koçan, Kerem Kaya, İbrahim Özçeşmeci, B Şebnem Sesalan, Meltem Göksel, Mahmut Durmuş, Ayfer Kalkan Burat
In this study, morpholinoethoxy-substituted metal-free (3), zinc(II) (4) and indium(III) (5) phthalocyanines were synthesized. These phthalocyanines were converted to their water-soluble quaternized derivatives (3Q-5Q) using excess methyl iodide as a quaternization agent. All these phthalocyanines (Pcs) were characterized by elemental analysis and different spectroscopic methods such as FT-IR, (1)H NMR, UV-Vis and mass spectrometry. The photophysical and photochemical properties such as fluorescence and generation of singlet oxygen were investigated for determination of these phthalocyanines as photosensitizers in photodynamic therapy (PDT) applications...
December 2017: Journal of Biological Inorganic Chemistry: JBIC
Aneta D Petelska, Monika Naumowicz
The Langmuir monolayers of L-α-phosphatidylcholine from egg yolk were studied by Wilhelmy method. The surface pressure versus molecular area isotherm of lipid on pure water and different subphase (with a presence of divalent ions: Sr(2+), Cd(2+), Ba(2+), Pb(2+)) was obtained. The limiting area of the isotherms depends on the presence of subphase ions. The addition of divalent ions to the subphase stabilized the monolayers and increased the limiting areas of the monolayer. During the compression in monolayer complexes of 1:1 and 2:1 stoichiometry between L-α-phosphatidylcholine from egg yolk and divalent ions are formed...
December 2017: Journal of Biological Inorganic Chemistry: JBIC
Ganna V Kalayda, Maximilian Kullmann, Markus Galanski, Sabrina Gollos
Oxaliplatin is the backbone of chemotherapy for advanced colorectal cancer and undergoes clinical trials for treatment of other tumour entities. However, acquired resistance is a major hurdle. Confocal microscopy is a useful tool to get an insight into the mechanisms of resistance but it requires fluorescent compounds. This work describes the synthesis of the novel oxaliplatin derivative (CFDA-oxPt) featuring 5(6)-carboxyfluorescein diacetate and evaluation of its applicability for the investigation of oxaliplatin resistance using imaging techniques...
December 2017: Journal of Biological Inorganic Chemistry: JBIC
Sebastian M Fica-Contreras, Sydney O Shuster, Nathaniel D Durfee, Gregory J K Bowe, Nathaniel J Henning, Staci A Hill, Geoffrey D Vrla, David R Stillman, Kelly M Suralik, Roger K Sandwick, Sunhee Choi
Extensive research has linked the amyloid-beta (Aβ) peptide to neurological dysfunction in Alzheimer's disease (AD). Insoluble Aβ plaques in the AD patient brain contain high concentrations of advanced glycation end-products (AGEs) as well as transition metal ions. This research elucidated the roles of Aβ, sugars, and Cu(2+) in the oxidative stress mechanism of AD at the molecular level. Mass spectral (MS) analysis of the reactions of Aβ with two representative sugars, ribose-5-phosphate (R5P) and methylglyoxal (MG), revealed Lys-16 and Arg-5 as the primary glycation sites...
December 2017: Journal of Biological Inorganic Chemistry: JBIC
Razak Hussain, Indu Kumari, Shikha Sharma, Mushtaq Ahmed, Tabreiz Ahmad Khan, Yusuf Akhter
Trichothecenes are the secondary metabolites produced by Trichoderma spp. Some of these molecules have been reported for their ability to stimulate plant growth by suppressing plant diseases and hence enabling Trichoderma spp. to be efficiently used as biocontrol agents in modern agriculture. Many of the proteins involved in the trichothecenes biosynthetic pathway in Trichoderma spp. are encoded by the genes present in the tri cluster. Tri4 protein catalyzes three consecutive oxygenation reaction steps during biosynthesis of isotrichodiol in the trichothecenes biosynthetic pathway, while tri11 protein catalyzes the C4 hydroxylation of 12, 13-epoxytrichothec-9-ene to produce trichodermol...
December 2017: Journal of Biological Inorganic Chemistry: JBIC
Chiara Di Bari, Nicolas Mano, Sergey Shleev, Marcos Pita, Antonio L De Lacey
An infrared spectroelectrochemical study of Trametes hirsuta laccase and Magnaporthe oryzae bilirubin oxidase has been performed using azide, an inhibitor of multicopper oxidases, as an active infrared probe incorporated into the T2/T3 copper cluster of the enzymes. The redox potential-controlled measurements indicate that N3(-) stretching IR bands of azide ion bound to the T2/T3 cluster are only detected for the oxidized enzymes, confirming that azide only binds to Cu(2+). Moreover, the process of binding/dissociation of azide ion is shown to be reversible...
December 2017: Journal of Biological Inorganic Chemistry: JBIC
Erhan Süleymanoğlu
Despite the significant efforts towards applying improved non-destructive and label-free measurements of biomolecular structures of lipid-based gene delivery vectors, little is achieved in terms of their structural relevance in gene transfections. Better understanding of structure-activity relationships of lipid-DNA complexes and their gene expression efficiencies thus becomes an essential issue. Raman scattering offers a complimentary measurement technique for following the structural transitions of both DNA and lipid vesicles employed for their transfer...
December 2017: Journal of Biological Inorganic Chemistry: JBIC
Paolo Ascenzi, Alessandra Pesce
Truncated hemoglobins (trHb) are present in protozoa, cyanobacteria, nemertean, bacteria, algae, and plants. They are characterized by the 2-on-2 topology and are ordered in four phylogenetic groups (I or N, II or O, III or P, and IV or Q). Several functions have been attributed to trHbs including the inactivation of reactive nitrogen and oxygen species, permitting the survival of microorganisms in the host. Here, the kinetics of peroxynitrite scavenging by ferric Campylobacter jejuni truncated hemoglobin P [i...
December 2017: Journal of Biological Inorganic Chemistry: JBIC
Zizhen Li, Ali Dergham, Holly McCulloch, Yubo Qin, Xiuying Yang, Jingchang Zhang, Xudong Cao
Each imaging modality has its own merits and intrinsic limitations; therefore, combining two or more complementary imaging modalities has become an interesting area of research. Recently, magnetic ion-doped quantum dots have become an increasingly promising class of optical/magnetic resonance multimodal imaging probes due to their excellent physical and chemical properties. In this work, Gd-doped CdTe quantum dots (QDs) were successfully synthesized via a facile one-step refluxing route,and their optimal synthesis conditions were investigated...
December 2017: Journal of Biological Inorganic Chemistry: JBIC
Joel C Bucci, Carlee S McClintock, Yuzhuo Chu, Gregory L Ware, Kayla D McConnell, Joseph P Emerson, Cynthia B Peterson
Components of the fibrinolytic system are subjected to stringent control to maintain proper hemostasis. Central to this regulation is the serpin plasminogen activator inhibitor-1 (PAI-1), which is responsible for specific and rapid inhibition of fibrinolytic proteases. Active PAI-1 is inherently unstable and readily converts to a latent, inactive form. The binding of vitronectin and other ligands influences stability of active PAI-1. Our laboratory recently observed reciprocal effects on the stability of active PAI-1 in the presence of transition metals, such as copper, depending on the whether vitronectin was also present (Thompson et al...
October 2017: Journal of Biological Inorganic Chemistry: JBIC
Dharmasivam Mahendiran, Raju Senthil Kumar, Vijayan Viswanathan, Devadasan Velmurugan, Aziz Kalilur Rahiman
The bis(4'-(4-tolyl)-2,2':6',2″-terpyridine)copper(II) complex [Cu(ttpy)2]Cl2 was synthesized and authenticated by single crystal analysis, which shows distorted octahedral geometry around copper(II) ion. The crystal packing is stabilized by C-H···π inter and intramolecular interactions. The complex was found to be lipophilic as determined by shake-flask method. In vitro cytotoxicity of the complex was tested against Ehrlich ascites carcinoma (EAC) and L6 myotube cell lines. The complex exhibit potent cytotoxicity with respect to the commercially available anticancer drug cisplatin...
October 2017: Journal of Biological Inorganic Chemistry: JBIC
Brian Lukaszewicz, Eliza McColl, Janet Yee, Steven Rafferty, Manon Couture
Flavohemoglobins are microbial enzymes that counter nitrosative stress, but the details of their underlying enzymatic activities and structure-function relationships are not completely understood. These enzymes have been identified in Gram-negative bacteria, certain fungi, and the parasitic protist Giardia intestinalis (gFlHb) which, despite lacking the ability to make heme, encodes several hemeproteins. To gain knowledge about the biophysical properties of the active site of gFlHb, we used resonance Raman spectroscopy to probe the wild-type protein and variants at globin domain positions E11, E7, and B10 on the distal, ligand-binding side of the heme...
October 2017: Journal of Biological Inorganic Chemistry: JBIC
Sambuddha Sen, J A Cowan
Monothiol glutaredoxins (Grx) serve as intermediate cluster carriers in iron-sulfur cluster trafficking. The [2Fe-2S]-bound holo forms of Grx proteins display cysteinyl coordination from exogenous glutathione (GSH), in addition to contact from protein-derived Cys. Herein, we report mechanistic studies that investigate the role of exogenous glutathione in defining cluster chirality, ligand exchange, and the cluster transfer chemistry of Saccharomyces cerevisiae Grx3. Systematic perturbations were introduced to the glutathione-binding site by substitution of conserved charged amino acids that form crucial electrostatic contacts with the glutathione molecule...
October 2017: Journal of Biological Inorganic Chemistry: JBIC
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