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Journal of Biomolecular Screening

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https://www.readbyqxmd.com/read/27932699/a-systematic-investigation-of-the-best-buffers-for-use-in-screening-by-maldi-mass-spectrometry
#1
Jessica Chandler, Carl Haslam, Neil Hardy, Melanie Leveridge, Peter Marshall
Matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) offers a label-free alternative for the screening of biochemical targets in both 1536- and 6144-assay formats, as well as potentially providing increased sensitivity, reproducibility, and the simultaneous detection of multiple assay components within a specified m/z range. Ion suppression effects are one of the principal limitations reported for MS analysis. Within MALDI-MS screening, it has been identified that certain biochemical components incorporated into the assay (e...
December 8, 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27932697/htra3-isoform-specific-elisas-for-early-detection-of-preeclampsia
#2
Yao Wang, Ying Li, Jonathan Hyett, Fabricio da Silva Costa, Guiying Nie
Preeclampsia is a serious disorder of human pregnancy occurring after 20 weeks of gestation. It can be divided into subtypes of early onset (<34 weeks of gestation) and late onset (>34 weeks). Presymptomatic detection to identify those at high risk is important for managing this disease. HtrA3, a serine protease with high expression in the developing placenta, exists in long (HtrA3-L) and short (HtrA3-S) isoforms. They are identical, except HtrA3-S lacks the C-terminal PDZ domain. We have previously shown by Western blot analysis that serum HtrA3 levels at the end of the first trimester are significantly higher in women who later develop preeclampsia than in controls...
December 8, 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/28139961/functional-comparison-of-neuronal-cells-differentiated-from-human-induced-pluripotent-stem-cell-derived-neural-stem-cells-under-different-oxygen-and-medium-conditions
#3
Kazuto Yamazaki, Kazuyuki Fukushima, Michiko Sugawara, Yoshikuni Tabata, Yoichi Imaizumi, Yasuharu Ishihara, Masashi Ito, Kappei Tsukahara, Jun Kohyama, Hideyuki Okano
Because neurons are difficult to obtain from humans, generating functional neurons from human induced pluripotent stem cells (hiPSCs) is important for establishing physiological or disease-relevant screening systems for drug discovery. To examine the culture conditions leading to efficient differentiation of functional neural cells, we investigated the effects of oxygen stress (2% or 20% O2 ) and differentiation medium (DMEM/F12:Neurobasal-based [DN] or commercial [PhoenixSongs Biologicals; PS]) on the expression of genes related to neural differentiation, glutamate receptor function, and the formation of networks of neurons differentiated from hiPSCs (201B7) via long-term self-renewing neuroepithelial-like stem (lt-NES) cells...
December 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/28139960/development-of-an-in-vitro-model-to-screen-cyp1b1-targeted-anticancer-prodrugs
#4
Zhiying Wang, Yao Chen, Laura M Drbohlav, Judy Qiju Wu, Michael Zhuo Wang
Cytochrome P450 1B1 (CYP1B1) is an anticancer therapeutic target due to its overexpression in a number of steroid hormone-related cancers. One anticancer drug discovery strategy is to develop prodrugs specifically activated by CYP1B1 in malignant tissues to cytotoxic metabolites. Here, we aimed to develop an in vitro screening model for CYP1B1-targeted anticancer prodrugs using the KLE human endometrial carcinoma cell line. KLE cells demonstrated superior stability of CYP1B1 expression relative to transiently transfected cells and did not express any appreciable amount of cognate CYP1A1 or CYP1A2, which would have compromised the specificity of the screening assay...
December 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27647668/one-step-seeding-of-neural-stem-cells-with-vitronectin-supplemented-medium-for-high-throughput-screening-assays
#5
Sheng Dai, Rong Li, Yan Long, Steve Titus, Jinghua Zhao, Ruili Huang, Menghang Xia, Wei Zheng
Human neuronal cells differentiated from induced pluripotent cells have emerged as a new model system for the study of disease pathophysiology and evaluation of drug efficacy. Differentiated neuronal cells are more similar in genetics and biological content to human brain cells than other animal disease models. However, culture of neuronal cells in assay plates requires a labor-intensive procedure of plate precoating, hampering its applications in high-throughput screening (HTS). We developed a simplified method with one-step seeding of neural stem cells in assay plates by supplementing the medium with a recombinant human vitronectin (VTN), thus avoiding plate precoating...
December 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27601436/matrix-based-activity-pattern-classification-as-a-novel-method-for-the-characterization-of-enzyme-inhibitors-derived-from-high-throughput-screening
#6
Douglas S Auld, Marta Jimenez, Kimberley Yue, Scott Busby, Yu-Chi Chen, Scott Bowes, Greg Wendel, Thomas Smith, Ji-Hu Zhang
One of the central questions in the characterization of enzyme inhibitors is determining the mode of inhibition (MOI). Classically, this is done with a number of low-throughput methods in which inhibition models are fitted to the data. The ability to rapidly characterize the MOI for inhibitors arising from high-throughput screening in which hundreds to thousands of primary inhibitors may need to be characterized would greatly help in lead selection efforts. Here we describe a novel method for determining the MOI of a compound without the need for curve fitting of the enzyme inhibition data...
December 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27572608/functional-characterization-of-acetylcholine-receptors-expressed-in-human-neurons-differentiated-from-hippocampal-neural-stem-progenitor-cells
#7
Kazuyuki Fukushima, Kazuto Yamazaki, Norimasa Miyamoto, Kohei Sawada
Neurotransmission mediated by acetylcholine receptors (AChRs) plays an important role in learning and memory functions in the hippocampus. Impairment of the cholinergic system contributes to Alzheimer's disease (AD), indicating the importance of AChRs as drug targets for AD. To improve the success rates for AD drug development, human cell models that mimic the target brain region are important. Therefore, we characterized the functional expression of nicotinic and muscarinic AChRs (nAChRs and mAChRs, respectively) in human hippocampal neurons differentiated from hippocampal neural stem/progenitor cells (HIP-009 cells)...
December 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27455994/rethinking-nuclear-receptors-as-potential-therapeutic-targets-for-retinal-diseases
#8
REVIEW
Mayur Choudhary, Goldis Malek
Collectively, retinal diseases, including age-related macular degeneration, retinitis pigmentosa, and diabetic retinopathy, result in severe vision impairment worldwide. The absence and/or limited availability of successful drug therapies for these blinding disorders necessitates further understanding their pathobiology and identifying new targetable signaling pathways. Nuclear receptors are transcription regulators of many key aspects of human physiology, as well as pathophysiology, with reported roles in development, aging, and disease...
December 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27455993/combining-unique-multiplex-gateway-cloning-and-bimolecular-fluorescence-complementation-bifc-for-high-throughput-screening-of-protein-protein-interactions
#9
Adriana Lepur, Lucija Kovačević, Robert Belužić, Oliver Vugrek
Protein interaction networks are the basis for human metabolic and signaling systems. Interaction studies often use bimolecular fluorescence complementation (BiFC) to reveal the formation and cellular localization of protein complexes. However, large-scale studies were either far from native conditions in human cells or limited by laborious restriction/ligation cloning techniques. Here, we describe a new tool for protein interaction screening based on Gateway-compatible BiFC vectors. We made a set of four new vectors that permit fusion of candidate proteins to the N or C fragment of Venus in all fusion positions...
December 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27412534/in-vitro-assays-for-the-discovery-of-pcsk9-autoprocessing-inhibitors
#10
Scott P Salowe, Lei Zhang, Hratch J Zokian, Jennifer J Gesell, Deborah L Zink, Judyann Wiltsie, Xi Ai, Michael Kavana, Shirly Pinto
PCSK9 plays a significant role in regulating low-density lipoprotein (LDL) cholesterol levels and has become an important drug target for treating hypercholesterolemia. Although a member of the serine protease family, PCSK9 only catalyzes a single reaction, the autocleavage of its prodomain. The maturation of the proprotein is an essential prerequisite for the secretion of PCSK9 to the extracellular space where it binds the LDL receptor and targets it for degradation. We have found that a construct of proPCSK9 where the C-terminal domain has been truncated has sufficient stability to be expressed and purified from Escherichia coli for the in vitro study of autoprocessing...
December 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27412533/identification-of-positive-allosteric-modulators-of-glycine-receptors-from-a-high-throughput-screen-using-a-fluorescent-membrane-potential-assay
#11
Clara Stead, Adam Brown, Cathryn Adams, Sarah J Nickolls, Gareth Young, Juha Kammonen, David Pryde, Darren Cawkill
Glycine receptor 3 (GlyRα3) is a ligand-gated ion channel of the cys-loop family that plays a key role in mediating inhibitory neurotransmission and regulation of pain signaling in the dorsal horn. Potentiation of GlyRα3 function is therefore of interest as a putative analgesic mechanism with which to target new therapeutics. However, to date, positive allosteric modulators (PAMs) of this receptor with sufficient selectivity to enable target validation studies have not been described. To address this lack of pharmacological tools, we developed a suite of in vitro assays comprising a high-throughput fluorescent membrane potential screen and a medium-throughput electrophysiology assay using IonFlux HT together with conventional manual patch clamp...
December 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27401582/early-perspective
#12
REVIEW
Brinton Seashore-Ludlow, Thomas Lundbäck
The cellular thermal shift assay (CETSA) was introduced in 2013 as a means to assess drug binding in complex environments such as cell lysates, live cells, and even tissues. The assay principle relies on the well-proven biophysical concept of ligand-induced thermal stabilization of proteins, which in CETSA applications is measured as a persistent presence of soluble protein at elevated temperatures. Given its recent development, we have just started to learn about the benefits and pitfalls of the method as it is applied to a growing number of protein target classes, the majority of which are intracellular soluble proteins...
December 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27269812/a-platform-to-enable-the-pharmacological-profiling-of-small-molecules-in-gel-based-electrophoretic-mobility-shift-assays
#13
Timothy L Foley, Dorjbal Dorjsuren, Thomas S Dexheimer, Michael D Burkart, William C Wight, Anton Simeonov
We describe a polyacrylamide gel casting cassette that overcomes limitations of commercially available gel electrophoresis equipment. This apparatus molds a single polyacrylamide gel that can evaluate more than 200 samples in parallel, is loaded with a multichannel pipettor, and is flexible with respect to composition of the separating matrix. We demonstrate its use to characterize inhibitors of enzymes that modify protein and nucleic acid substrates. Throughputs of greater than 1000 samples per day were achieved when this system was paired with a quantitative laser-based imaging system, yielding data of remarkable quality...
December 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27650791/high-throughput-platform-for-identifying-molecular-factors-involved-in-phenotypic-stabilization-of-primary-human-hepatocytes-in-vitro
#14
Jing Shan, David J Logan, David E Root, Anne E Carpenter, Sangeeta N Bhatia
Liver disease is a leading cause of morbidity worldwide and treatment options are limited, with organ transplantation being the only form of definitive management. Cell-based therapies have long held promise as alternatives to whole-organ transplantation but have been hindered by the rapid loss of liver-specific functions over a period of days in cultured hepatocytes. Hypothesis-driven studies have identified a handful of factors that modulate hepatocyte functions in vitro, but our understanding of the mechanisms involved remains incomplete...
October 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27650790/slas-europe-high-content-screening-conference-in-dresden-a-glimpse-of-the-future
#15
EDITORIAL
Maria Montoya, Thierry Dorval, Marc Bickle
No abstract text is available yet for this article.
October 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27461835/development-of-a-high-throughput-gene-expression-screen-for-modulators-of-ras-mapk-signaling-in-a-mutant-ras-cellular-context
#16
Bryan Severyn, Thi Nguyen, Michael D Altman, Lixia Li, Kumiko Nagashima, George N Naumov, Sriram Sathyanarayanan, Erica Cook, Erick Morris, Marc Ferrer, Bill Arthur, Yair Benita, Jim Watters, Andrey Loboda, Jeff Hermes, D Gary Gilliland, Michelle A Cleary, Pamela M Carroll, Peter Strack, Matt Tudor, Jannik N Andersen
The RAS-MAPK pathway controls many cellular programs, including cell proliferation, differentiation, and apoptosis. In colorectal cancers, recurrent mutations in this pathway often lead to increased cell signaling that may contribute to the development of neoplasms, thereby making this pathway attractive for therapeutic intervention. To this end, we developed a 26-member gene signature of RAS-MAPK pathway activity utilizing the Affymetrix QuantiGene Plex 2.0 reagent system and performed both primary and confirmatory gene expression-based high-throughput screens (GE-HTSs) using KRAS mutant colon cancer cells (SW837) and leveraging a highly annotated chemical library...
October 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27412535/development-of-a-3d-tissue-culture-based-high-content-screening-platform-that-uses-phenotypic-profiling-to-discriminate-selective-inhibitors-of-receptor-tyrosine-kinases
#17
Tijmen H Booij, Maarten J D Klop, Kuan Yan, Csaba Szántai-Kis, Balint Szokol, Laszlo Orfi, Bob van de Water, Gyorgy Keri, Leo S Price
3D tissue cultures provide a more physiologically relevant context for the screening of compounds, compared with 2D cell cultures. Cells cultured in 3D hydrogels also show complex phenotypes, increasing the scope for phenotypic profiling. Here we describe a high-content screening platform that uses invasive human prostate cancer cells cultured in 3D in standard 384-well assay plates to study the activity of potential therapeutic small molecules and antibody biologics. Image analysis tools were developed to process 3D image data to measure over 800 phenotypic parameters...
October 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27358388/identification-of-novel-inhibitors-of-the-type-i-interferon-induction-pathway-using-cell-based-high-throughput-screening
#18
Zoe O Gage, Andri Vasou, David W Gray, Richard E Randall, Catherine S Adamson
Production of type I interferon (IFN) is an essential component of the innate immune response against invading pathogens. However, its production must be tightly regulated to avoid harmful effects. Compounds that modulate the IFN response are potentially valuable for a variety of applications due to IFN's beneficial and detrimental roles. We developed and executed a cell-based high-throughput screen (HTS) targeting components that participate in and/or regulate the IRF3 and nuclear factor (NF)-κB branches of the IFN induction pathway...
October 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27256155/a-novel-automated-high-content-analysis-workflow-capturing-cell-population-dynamics-from-induced-pluripotent-stem-cell-live-imaging-data
#19
Maximilian Kerz, Amos Folarin, Ruta Meleckyte, Fiona M Watt, Richard J Dobson, Davide Danovi
Most image analysis pipelines rely on multiple channels per image with subcellular reference points for cell segmentation. Single-channel phase-contrast images are often problematic, especially for cells with unfavorable morphology, such as induced pluripotent stem cells (iPSCs). Live imaging poses a further challenge, because of the introduction of the dimension of time. Evaluations cannot be easily integrated with other biological data sets including analysis of endpoint images. Here, we present a workflow that incorporates a novel CellProfiler-based image analysis pipeline enabling segmentation of single-channel images with a robust R-based software solution to reduce the dimension of time to a single data point...
October 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27233291/assay-establishment-and-validation-of-a-high-throughput-screening-platform-for-three-dimensional-patient-derived-colon-cancer-organoid-cultures
#20
Karsten Boehnke, Philip W Iversen, Dirk Schumacher, María José Lallena, Rubén Haro, Joaquín Amat, Johannes Haybaeck, Sandra Liebs, Martin Lange, Reinhold Schäfer, Christian R A Regenbrecht, Christoph Reinhard, Juan A Velasco
The application of patient-derived three-dimensional culture systems as disease-specific drug sensitivity models has enormous potential to connect compound screening and clinical trials. However, the implementation of complex cell-based assay systems in drug discovery requires reliable and robust screening platforms. Here we describe the establishment of an automated platform in 384-well format for three-dimensional organoid cultures derived from colon cancer patients. Single cells were embedded in an extracellular matrix by an automated workflow and subsequently self-organized into organoid structures within 4 days of culture before being exposed to compound treatment...
October 2016: Journal of Biomolecular Screening
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