journal
MENU ▼
Read by QxMD icon Read
search

Molecules and Cells

journal
https://www.readbyqxmd.com/read/29281779/inhibition-of-autolysis-by-lipase-lipa-in-streptococcus-pneumoniae-sepsis
#1
Gyu-Lee Kim, Truc Thanh Luong, Sang-Sang Park, Seungyeop Lee, Jung Ah Ha, Cuong Thach Nguyen, Ji Hye Ahn, Ki-Tae Park, Man-Jeong Paik, Suhkneung Pyo, David E Briles, Dong-Kwon Rhee
More than 50% of sepsis cases are associated with pneumonia. Sepsis is caused by infiltration of bacteria into the blood via inflammation, which is triggered by the release of cell wall components following lysis. However, the regulatory mechanism of lysis during infection is not well defined. Mice were infected with Streptococcus pneumoniae D39 wild-type (WT) and lipase mutant (ΔlipA) intranasally (pneumonia model) or intraperitoneally (sepsis model), and survival rate and pneumococcal colonization were determined...
December 26, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29276941/rnf152-is-essential-for-neurod-expression-and-delta-notch-signaling-in-the-zebrafish-embryos
#2
Ajeet Kumar, Tae-Lin Huh, Joonho Choe, Myungchull Rhee
We report the biological functions of a zebrafish homologue of RING-finger protein 152 (rnf152) during embryogenesis. rnf152 was initially identified as a brain-enriched E3 ligase involved in early embryogenesis of zebrafish. Expression of rnf152 was ubiquitous in the brain at 24 hpf but restricted to the eyes, midbrain-hindbrain boundary (MHB), and rhombomeres at 48 hpf. Knockdown of rnf152 in zebrafish embryos caused defects in the eyes, MHB, and rhombomeres (r1-7) at 24 hpf. These defects in rnf152-deficient embryos were analyzed by whole-mount in situ hybridization (WISH) using neuroD, deltaD, notch1a, and notch3 probes...
December 22, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29276943/nuclear-bodies-built-on-architectural-long-noncoding-rnas-unifying-principles-of-their-construction-and-function
#3
Takeshi Chujo, Tetsuro Hirose
Nuclear bodies are subnuclear, spheroidal, and membraneless compartments that concentrate specific proteins and/or RNAs. They serve as sites of biogenesis, storage, and sequestration of specific RNAs, proteins, or ribonucleoprotein complexes. Recent studies reveal that a subset of nuclear bodies in various eukaryotic organisms is constructed using architectural long noncoding RNAs (arcRNAs). Here, we describe the unifying mechanistic principles of the construction and function of these bodies, especially focusing on liquid-liquid phase separation induced by architectural molecules that form multiple weakly adhesive interactions...
December 20, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29276942/humic-acid-confers-high-affinity-k-transporter-1-mediated-salinity-stress-tolerance-in-arabidopsis
#4
Laila Khaleda, Hee Jin Park, Dae-Jin Yun, Jong-Rok Jeon, Min Gab Kim, Joon-Yung Cha, Woe-Yeon Kim
Excessive salt disrupts intracellular ion homeostasis and inhibits plant growth, which poses a serious threat to global food security. Plants have adapted various strategies to survive in unfavorable saline soil conditions. Here, we show that humic acid (HA) is a good soil amendment that can be used to help overcome salinity stress because it markedly reduces the adverse effects of salinity on Arabidopsis thaliana seedlings. To identify the molecular mechanisms of HA-induced salt stress tolerance in Arabidopsis, we examined possible roles of a sodium influx transporter HIGH-AFFINITY K+ TRANSPORTER 1 (HKT1)...
December 20, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29237257/iron-homeostasis-controls-myeloid-blood-cell-differentiation-in-drosophila
#5
Sunggyu Yoon, Bumsik Cho, Mingyu Shin, Ferdinand Koranteng, Nuri Cha, Jiwon Shim
Iron is an essential divalent ion for aerobic life. Life has evolved to maintain iron homeostasis for normal cellular and physiological functions and therefore imbalances in iron levels exert a wide range of consequences. Responses to iron dysregulation in blood development, however, remain elusive. Here, we found that iron homeostasis is critical for differentiation of Drosophila blood cells in the larval hematopoietic organ, called the lymph gland. Supplementation of an iron chelator, bathophenanthroline disulfate (BPS) results in an excessive differentiation of the crystal cell in the lymph gland...
December 14, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29237256/microrna-206-protects-against-myocardial-ischaemia-reperfusion-injury-in-rats-by-targeting-gadd45%C3%AE
#6
Changlin Zhai, Qang Qian, Guanmin Tang, Bingjiang Han, Huilin Hu, Dong Yin, Haihua Pan, Song Zhang
MicroRNAs are widely involved in the pathogenesis of cardiovascular diseases through regulating gene expression via translational inhibition or degradation of their target mRNAs. Recent studies have indicated a critical role of microRNA-206 in myocardial ischaemia-reperfusion (I/R) injury. However, the function of miR-206 in myocardial I/R injury is currently unclear. The present study was aimed to identify the specific role of miR-206 in myocardial I/R injury and explore the underlying molecular mechanism...
December 14, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29237115/isolation-and-functional-examination-of-the-long-non-coding-rna-redrum
#7
Yerim Lee, Charny Park, Sanghyuk Lee, Daekee Lee, Jaesang Kim
Here, we report isolation of multiple long non-coding RNAs (lncRNAs) expressed tissue-specifically during murine embryogenesis. One of these, subsequently came to be known as Redrum, is expressed in erythropoietic cells in fetal liver and adult bone marrow. Redrum transcription is also detected during pregnancy in the spleen where extramedullary hematopoiesis takes place. In order to examine the function of Redrum in vivo, we generated a gene-targeted murine model and analyzed its embryonic and adult erythropoiesis...
December 12, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29237114/emerging-paradigm-of-crosstalk-between-autophagy-and-the-ubiquitin-proteasome-system
#8
Taewook Nam, Jong Hyun Han, Sushil Devkota, Han-Woong Lee
Cellular protein homeostasis is maintained by two major degradation pathways, namely the ubiquitin-proteasome system (UPS) and autophagy. Until recently, the UPS and autophagy were considered to be largely independent systems targeting proteins for degradation in the proteasome and lysosome, respectively. However, the identification of crucial roles of molecular players such as ubiquitin and p62 in both of these pathways as well as the observation that blocking the UPS affects autophagy flux and vice versa has generated interest in studying crosstalk between these pathways...
December 12, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29237113/the-replication-protein-cdc6-suppresses-centrosome-over-duplication-in-a-manner-independent-of-its-atpase-activity
#9
Gwang Su Kim, Inyoung Lee, Ji Hun Kim, Deog Su Hwang
The Cdc6 protein is essential for the initiation of chromosomal replication and functions as a licensing factor to maintain chromosome integrity. During the S and G2 phases of the cell cycle, Cdc6 has been found to inhibit the recruitment of pericentriolar material (PCM) proteins to the centrosome and to suppress centrosome over-duplication. In this report, we analyzed the correlation between these two functions of Cdc6 at the centrosome. Cdc6 depletion increased the population of cells showing centrosome over-duplication and premature centrosome separation; Cdc6 expression reversed these changes...
December 12, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29179263/odorant-receptors-containing-conserved-amino-acid-sequences-in-transmembrane-domain-7-display-distinct-expression-patterns-in-mammalian-tissues
#10
Sang Eun Ryu, Tammy Shim, Ju-Yeon Yi, So Yeun Kim, Sun Hwa Park, Sung Won Kim, Gabriele V Ronnett, Cheil Moon
Mammalian genomes are well established, and highly conserved regions within odorant receptors that are unique from other G-protein coupled receptors have been identified. Numerous functional studies have focused on specific conserved amino acids motifs, however, not all conserved motifs have been sufficiently characterized. Here, we identified a highly conserved 18 amino acid sequence motif within transmembrane domain seven (CAS-TM7) which was identified by aligning odorant receptor sequences. Next, we investigated the expression pattern and distribution of this conserved amino acid motif among a broad range of odorant receptors...
November 23, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29179262/advances-towards-controlling-meiotic-recombination-for-plant-breeding
#11
Kyuha Choi
Meiotic homologous recombination generates new combinations of preexisting genetic variation and is a crucial process in plant breeding. Within the last decade, our understanding of plant meiotic recombination and genome diversity has advanced considerably. Innovation in DNA sequencing technology has led to the exploration of high-resolution genetic and epigenetic information in plant genomes, which has helped to accelerate plant breeding practices via high-throughput genotyping, and linkage and association mapping...
November 23, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29179261/targeted-base-editing-via-rna-guided-cytidine-deaminases-in-xenopus-laevis-embryos
#12
Dong-Seok Park, Mijung Yoon, Jiyeon Kweon, An-Hee Jang, Yongsub Kim, Sun-Cheol Choi
Genome editing using programmable nucleases such as CRISPR/Cas9 or Cpf1 has emerged as powerful tools for gene knock-out or knock-in in various organisms. While most genetic diseases are caused by point mutations, these genome-editing approaches are inefficient in inducing single-nucleotide substitutions. Recently, Cas9-linked cytidine deam-inases, named base editors (BEs), have been shown to convert cytidine to uridine efficiently, leading to targeted single-base pair substitutions in human cells and organisms...
November 20, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29145720/phospholipase-d-and-its-essential-role-in-cancer
#13
Ju Hwan Cho, Joong-Soo Han
The role of Phospholipase D (PLD) in cancer development and management has been a major area of interest for researchers. The purpose of this mini-review is to explore PLD and its distinct role during chemotherapy including anti-apoptotic function. PLD is an enzyme that belongs to the phospholipase super family and is found in a broad range of organisms such as viruses, yeast, bacteria, animals, and plants. The function and activity of PLD are widely dependent on and regulated by neurotransmitters, hormones, small monomeric GTPases, and lipids...
November 16, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29145719/maintained-mpf-level-after-oocyte-vitrification-improves-embryonic-development-after-ivf-but-not-after-somatic-cell-nuclear-transfer
#14
Ji I Baek, Dong-Won Seol, Ah-Reum Lee, Woo Sik Lee, Sook-Young Yoon, Dong Ryul Lee
Levels of maturation-promoting factor (MPF) in oocytes decline after vitrification, and this decline has been suggested as one of the main causes of low developmental competence resulting from cryoinjury. Here, we evaluated MPF activity in vitrified mouse eggs following treatment with caffeine, a known stimulator of MPF activity, and/or the proteasome inhibitor MG132. Collected MII oocytes were vitrified and divided into four groups: untreated, 10 mM caffeine (CA), 10 μM MG132 (MG), and 10 mM caffeine +10 μM MG132 (CA+MG)...
November 16, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29145718/lipopolysaccharide-lps-induced-autophagy-is-responsible-for-enhanced-osteoclastogenesis
#15
Ok-Joo Sul, Hyun-Jung Park, Ho-Jung Son, Hye-Seon Choi
We hypothesized that inflammation affects number and activity of osteoclasts (OCs) via enhancing autophagy. Lipopolysaccharide (LPS) induced autophagy, osteoclastogenesis, and cytoplasmic reactive oxygen species (ROS) in bone marrowderived macrophages that were pre-stimulated with receptor activator of nuclear factor-κB ligand. An autophagy inhibitor, 3-methyladenine (3-MA) decreased LPS-induced OC formation and bone resorption, indicating that autophagy is responsible for increasing number and activity of OCs upon LPS stimulus...
November 16, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29145717/ctrp9-regulates-growth-differentiation-and-apoptosis-in-human-keratinocytes-through-tgf%C3%AE-1-p38-dependent-pathway
#16
Tae Woo Jung, Hyung Sub Park, Geum Hee Choi, Daehwan Kim, Taeseung Lee
Impairment of wound healing is a common problem in individuals with diabetes. Adiponectin, an adipocyte-derived cytokine, has many beneficial effects on metabolic disorders such as diabetes, obesity, hypertension, and dyslipidemia. C1q/TNF-Related Protein 9 (CTRP9), the closest paralog of adiponectin, has been reported to have beneficial effects on wound healing. In the current study, we demonstrate that CTRP9 regulates growth, differentiation, and apoptosis of HaCaT human keratinocytes. We found that CTRP9 augmented expression of transforming growth factor beta 1 (TGFβ1) by transcription factor activator protein 1 (AP-1) binding activity and phosphorylation of p38 in a dosedependent manner...
November 16, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29127951/sox12-is-a-cancer-stem-like-cell-marker-in-hepatocellular-carcinoma
#17
Song Zou, Chen Wang, Jiansheng Liu, Qun Wang, Dongdong Zhang, Shengnan Zhu, Shengyuan Xu, Mafei Kang, Shaozhong He
Recent studies on molecular carcinogenesis suggest that the chemo-resistance of some cancers is largely due to presence of cancer stem cells (CSCs), which affect the chemotherapy outcome for hepatocellular carcinoma (HCC). However, currently no consensus on a CSC phenotype in HCC has been obtained. Here, we examined Sox12 as a novel CSC marker in HCC. Sox12+ versus Sox12- cells were purified from HCC cell lines. The Sox12+ cells were compared with Sox12- HCC cells for tumor sphere formation, chemo-resistance, tumor formation after serial adoptive transplantations in nude mice, and the frequency of developing distal metastasis...
November 10, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29113429/endophilin-a2-a-potential-link-to-adiposity-and-beyond
#18
Assim A Alfadda, Reem M Sallam, Rukhsana Gul, Injae Hwang, Sojeong Ka
Adipose tissue plays a central role in regulating dynamic crosstalk between tissues and organs. A detailed description of molecules that are differentially expressed upon changes in adipose tissue mass is expected to increase our understanding of the molecular mechanisms that underlie obesity and related metabolic co-morbidities. Our previous studies suggest a possible link between endophilins (SH3Grb2 proteins) and changes in body weight. To explore this further, we sought to assess the distribution of endophilin A2 (EA2) in human adipose tissue and experimental animals...
November 6, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29113428/visualization-of-multicolored-in-vivo-organelle-markers-for-co-localization-studies-in-oryza-sativa
#19
Sarmina Dangol, Raksha Singh, Yafei Chen, Nam-Soo Jwa
Eukaryotic cells consist of a complex network of thousands of proteins present in different organelles where organellespecific cellular processes occur. Identification of the subcellular localization of a protein is important for understanding its potential biochemical functions. In the post-genomic era, localization of unknown proteins is achieved using multiple tools including a fluorescent-tagged protein approach. Several fluorescent-tagged protein organelle markers have been introduced into dicot plants, but its use is still limited in monocot plants...
November 6, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29081084/mitochondrial-uncoupling-attenuates-age-dependent-neurodegeneration-in-c-elegans
#20
Injeong Cho, Hyun-Ok Song, Jeong Hoon Cho
The uncoupling protein 4 (ucp-4) gene is involved in agedependent neurodegeneration in C. elegans. Therefore, we aimed to investigate the mechanism underlying the association between mitochondrial uncoupling and neurodegeneration by examining the effects of uncoupling agents and ucp-4 overexpression in C. elegans. Treatment with either DNP or CCCP improved neuronal defects in wild type during aging.Uncoupling agents also restored neuronal phenotypes of ucp-4 mutants to those exhibited by wild type, while ucp-4 overexpression attenuated the severity of age-dependent neurodegeneration...
October 27, 2017: Molecules and Cells
journal
journal
32586
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"