journal
MENU ▼
Read by QxMD icon Read
search

Molecules and Cells

journal
https://www.readbyqxmd.com/read/28427249/crystal-structure-of-the-regulatory-domain-of-aphb-from-vibrio-vulnificus-a-virulence-gene-regulator
#1
Nohra Park, Saemee Song, Garam Choi, Kyung Ku Jang, Inseong Jo, Sang Ho Choi, Nam-Chul Ha
The transcriptional activator AphB has been implicated in acid resistance and pathogenesis in the food borne pathogens Vibrio vulnificus and Vibrio cholerae. To date, the full-length AphB crystal structure of V. cholerae has been determined and characterized by a tetrameric assembly of AphB consisting of a DNA binding domain and a regulatory domain (RD). Although acidic pH and low oxygen tension might be involved in the activation of AphB, it remains unknown which ligand or stimulus activates AphB at the molecular level...
April 20, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28427248/caloric-restriction-induced-extension-of-chronological-lifespan-requires-intact-respiration-in-budding-yeast
#2
Young-Yon Kwon, Sung-Keun Lee, Cheol-Koo Lee
Caloric restriction (CR) has been shown to extend lifespan and prevent cellular senescence in various species ranging from yeast to humans. Many effects of CR may contribute to extend lifespan. Specifically, CR prevents oxidative damage from reactive oxygen species (ROS) by enhancing mitochondrial function. In this study, we characterized 33 single electron transport chain (ETC) gene-deletion strains to identify CR-induced chronological lifespan (CLS) extension mechanisms. Interestingly, defects in 17 of these 33 ETC genedeleted strains showed loss of both respiratory function and CR-induced CLS extension...
April 20, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28401751/down-regulation-of-microrna-210-confers-sensitivity-towards-1-s-1-acetoxychavicol-acetate-aca-in-cervical-cancer-cells-by-targeting-smad4
#3
Neoh Hun Phuah, Mohamad Nurul Azmi, Khalijah Awang, Noor Hasima Nagoor
MicroRNAs (miRNAs) are short non-coding RNAs that regulate genes posttranscriptionally. Past studies have reported that miR-210 is up-regulated in many cancers including cervical cancer, and plays a pleiotropic role in carcinogenesis. However, its role in regulating response towards anti-cancer agents has not been fully elucidated. We have previously reported that the natural compound 1'S-1'-acetoxychavicol acetate (ACA) is able to induce cytotoxicity in various cancer cells including cervical cancer cells...
April 12, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28401750/the-ciliary-transition-zone-finding-the-pieces-and-assembling-the-gate
#4
João Gonçalves, Laurence Pelletier
Eukaryotic cilia are organelles that project from the surface of cells to fulfill motility and sensory functions. In vertebrates, the functions of both motile and immotile cilia are critical for embryonic development and adult tissue homeostasis. Importantly, a multitude of human diseases is caused by abnormal cilia biogenesis and functions which rely on the compartmentalization of the cilium and the maintenance of its protein composition. The transition zone (TZ) is a specialized ciliary domain present at the base of the cilium and is part of a gate that controls protein entry and exit from this organelle...
April 12, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28359145/neuroprotective-effects-of-protein-tyrosine-phosphatase-1b-inhibition-against-er-stress-induced-toxicity
#5
Yu-Mi Jeon, Shinrye Lee, Seyeon Kim, Younghwi Kwon, Kiyoung Kim, Chang Geon Chung, Seongsoo Lee, Sung Bae Lee, Hyung-Jun Kim
Several lines of evidence suggest that endoplasmic reticulum (ER) stress plays a critical role in the pathogenesis of many neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis. Protein tyrosine phosphatase 1B (PTP1B) is known to regulate the ER stress signaling pathway, but its role in neuronal systems in terms of ER stress remains largely unknown. Here, we showed that rotenone-induced toxicity in human neuroblastoma cell lines and mouse primary cortical neurons was ameliorated by PTP1B inhibition...
March 28, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28359144/mind-bomb-binding-partner-ranbp9-plays-a-contributory-role-in-retinal-development
#6
Kyeong-Won Yoo, Maivannan Thiruvarangan, Yun-Mi Jeong, Mi-Sun Lee, Sateesh Maddirevula, Myungchull Rhee, Young-Ki Bae, Hyung-Goo Kim, Cheol-Hee Kim
Ran-binding protein family member, RanBP9 has been reported in various basic cellular mechanisms and neuropathological conditions including schizophrenia. Previous studies have reported that RanBP9 is highly expressed in the mammalian brain and retina; however, the role of RanBP9 in retinal development is largely unknown. Here, we present the novel and regulatory roles of RanBP9 in retinal development of a vertebrate animal model, zebrafish. Zebrafish embryos exhibited abundant expression of ranbp9 in developing brain tissues as well as in the developing retina...
March 28, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28320202/identification-and-expression-of-equine-mer-derived-mirnas
#7
Jeong-An Gim, Heui-Soo Kim
MicroRNAs (miRNAs) are single-stranded, small RNAs (21-23 nucleotides) that function in gene silencing and translational inhibition via the RNA interference mechanism. Most miRNAs originate from host genomic regions, such as intergenic regions, introns, exons, and transposable elements (TEs). Here, we focused on the palindromic structure of medium reiteration frequencies (MERs), which are similar to precursor miRNAs. Five MER consensus sequences (MER5A1, MER53, MER81, MER91C, and MER117) were matched with paralogous transcripts predicted to be precursor miRNAs in the horse genome (equCab2) and located in either intergenic regions or introns...
March 21, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28359147/functional-enhancers-as-master-regulators-of-tissue-specific-gene-regulation-and-cancer-development
#8
REVIEW
Je Yeong Ko, Sumin Oh, Kyung Hyun Yoo
Tissue-specific transcription is critical for normal development, and abnormalities causing undesirable gene expression may lead to diseases such as cancer. Such highly organized transcription is controlled by enhancers with specific DNA sequences recognized by transcription factors. Enhancers are associated with chromatin modifications that are distinct epigenetic features in a tissue-specific manner. Recently, super-enhancers comprising enhancer clusters co-occupied by lineage-specific factors have been identified in diverse cell types such as adipocytes, hair follicle stem cells, and mammary epithelial cells...
March 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28359146/transforming-growth-factor-%C3%AE-1-smad4-signaling-affects-osteoclast-differentiation-via-regulation-of-mir-155-expression
#9
Hongying Zhao, Jun Zhang, Haiyu Shao, Jianwen Liu, Mengran Jin, Jinping Chen, Yazeng Huang
Transforming growth factor β1 (TGFβ1)/Smad4 signaling plays a pivotal role in maintenance of the dynamic balance between bone formation and resorption. The microRNA miR-155 has been reported to exert a significant role in the differentiation of macrophage and dendritic cells. The goal of this study was to determine whether miR-155 regulates osteoclast differentiation through TGFβ1/Smad4 signaling. Here, we present that TGFβ1 elevated miR-155 levels during osteoclast differentiation through the stimulation of M-CSF and RANKL...
March 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28343379/hepatitis-c-virus-nonstructural-5a-protein-hcv-ns5a-inhibits-hepatocyte-apoptosis-through-the-nf-%C3%AE%C2%BAb-mir-503-bcl-2-pathway
#10
Zhengyuan Xie, Zhihua Xiao, Fenfen Wang
The nonstructural protein 5A (NS5A) encoded by the human hepatitis C virus (HCV) RNA genome is a multifunctional phosphoprotein. To analyse the influence of NS5A on apoptosis, we established an Hep-NS5A cell line (HepG2 cells that stably express NS5A) and induced apoptosis using tumour necrosis factor (TNF)-α. We utilised the MTT assay to detect cell viability, real-time quantitative polymerase chain reaction and Western blot to analyse gene and protein expression, and a luciferase reporter gene experiment to investigate the targeted regulatory relationship...
March 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28343378/adipose-derived-stem-cells-suppress-inflammation-induced-by-il-1%C3%AE-through-down-regulation-of-p2x7r-mediated-by-mir-373-in-chondrocytes-of-osteoarthritis
#11
Rilong Jin, Miaoda Shen, Liedao Yu, Xuanwei Wang, Xiangjin Lin
Adipose-derived stem cells (ADSCs) were previously considered to have an anti-inflammatory effect, and Interleukin-1β (IL-1β) was found to be a pro-inflammatory factor in chondrocytes, but the mechanism underlying ADSCs and IL-1β is unclear. In this study, we investigate whether P2X7 receptor (P2X7R) signalling, regulated by microRNA 373 (miR-373), was involved in the ADSCs and IL-1β mediated inflammation in osteoarthritis (OA). Chondrocytes were collected from 20 OA patients and 20 control participants, and ADSCs were collected from patients who had undergone abdominal surgery...
March 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28317368/mir-186-inhibited-migration-of-nsclc-via-targeting-cdc42-and-effecting-emt-process
#12
Ying Dong, Xintian Jin, Zhiqiang Sun, Yueming Zhao, Xianjing Song
In this study, qRT-PCR was employed to identify that miR-186 expression level in NSCLC tissues are highly associated with lymph node metastasis. In addition, through the application of western blotting, luciferase assay and qRT-PCR, it was found that miR-186 targeted 3'UTR of cdc42 mRNA and down-regulated cdc42 protein level in a post-transcriptional manner. Transwell assay indicated that cdc42 partially reversed the effect of miR-186 mimics. Besides, miR-186 was proved to regulate EMT by influencing biomarkers of this process and cell adhesion ability...
March 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28301917/signaling-pathways-controlling-microglia-chemotaxis
#13
REVIEW
Yang Fan, Lirui Xie, Chang Y Chung
Microglia are the primary resident immune cells of the central nervous system (CNS). They are the first line of defense of the brain's innate immune response against infection, injury, and diseases. Microglia respond to extracellular signals and engulf unwanted neuronal debris by phagocytosis, thereby maintaining normal cellular homeostasis in the CNS. Pathological stimuli such as neuronal injury induce transformation and activation of resting microglia with ramified morphology into a motile amoeboid form and activated microglia chemotax toward lesion site...
March 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28301916/a-role-of-central-nell2-in-the-regulation-of-feeding-behavior-in-rats
#14
Jin Kwon Jeong, Jae Geun Kim, Han Rae Kim, Tae Hwan Lee, Jeong Woo Park, Byung Ju Lee
A brain-enriched secreting signal peptide, NELL2, has been suggested to play multiple roles in the development, survival, and activity of neurons in mammal. We investigated here a possible involvement of central NELL2 in regulating feeding behavior and metabolism. In situ hybridization and an im-munohistochemical approach were used to determine expression of NELL2 as well as its colocalization with proopiomelanocortin (POMC) and neuropeptide Y (NPY) in the rat hypothalamus. To investigate the effect of NELL2 on feeding behavior, 2 nmole of antisense NELL2 oligodeoxynucleotide was administered into the lateral ventricle of adult male rat brains for 6 consecutive days, and changes in daily body weight, food, and water intake were monitored...
March 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28292004/transcription-factor-osdof18-controls-ammonium-uptake-by-inducing-ammonium-transporters-in-rice-roots
#15
Yunfei Wu, Wenzhu Yang, Jinhuan Wei, Hyeryung Yoon, Gynheung An
Nitrogen is one of the most important mineral elements for plant growth. We studied the functional roles of Oryza sativa DNA BINDING WITH ONE FINGER 18 (OsDOF18) in controlling ammonium uptake. The growth of null mutants of OsDOF18 was retarded in a medium containing ammonium as the sole nitrogen source. In contrast, those mutants grew normally in a medium with nitrate as the sole nitrogen source. The gene expression was induced by ammonium but not by nitrate. Uptake of ammonium was lower in osdof18 mutants than in the wild type, while that of nitrate was not affected by the mutation...
March 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28292003/arabidopsis-map3k16-and-other-salt-inducible-map3ks-regulate-aba-response-redundantly
#16
Seo-Wha Choi, Seul-Bee Lee, Yeon-Ju Na, Sun-Geum Jeung, Soo Young Kim
In the Arabidopsis genome, approximately 80 MAP3Ks (mitogen-activated protein kinase kinase kinases) have been identified. However, only a few of them have been characterized, and the functions of most MAP3Ks are largely unknown. In this paper, we report the function of MAP3K16 and several other MAP3Ks, MAP3K14/15/17/18, whose expression is salt-inducible. We prepared MAP3K16 overexpression (OX) lines and analyzed their phenotypes. The result showed that the transgenic plants were ABA-insensitive during seed germination and cotyledon greening stage but their root growth was ABA-hypersensitive...
March 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28241407/survival-assays-using-caenorhabditis-elegans
#17
REVIEW
Hae-Eun H Park, Yoonji Jung, Seung-Jae V Lee
Caenorhabditis elegans is an important model organism with many useful features, including rapid development and aging, easy cultivation, and genetic tractability. Survival assays using C. elegans are powerful methods for studying physiological processes. In this review, we describe diverse types of C. elegans survival assays and discuss the aims, uses, and advantages of specific assays. C. elegans survival assays have played key roles in identifying novel genetic factors that regulate many aspects of animal physiology, such as aging and lifespan, stress response, and immunity against pathogens...
February 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28241406/interferon-stimulated-gene-15-in-the-control-of-cellular-responses-to-genotoxic-stress
#18
REVIEW
Young Joo Jeon, Jong Ho Park, Chin Ha Chung
Error-free replication and repair of DNA are pivotal to organisms for faithful transmission of their genetic information. Cells orchestrate complex signaling networks that sense and resolve DNA damage. Post-translational protein modifications by ubiquitin and ubiquitin-like proteins, including SUMO and NEDD8, are critically involved in DNA damage response (DDR) and DNA damage tolerance (DDT). The expression of interferon-stimulated gene 15 (ISG15), the first identified ubiquitin-like protein, has recently been shown to be induced under various DNA damage conditions, such as exposure to UV, camptothecin, and doxorubicin...
February 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28222496/the-integrins-involved-in-soybean-agglutinin-induced-cell-cycle-alterations-in-ipec-j2
#19
Li Pan, Yuan Zhao, Zhijie Yuan, Mohammed Hamdy Farouk, Shiyao Zhang, Nan Bao, Guixin Qin
Soybean agglutinin (SBA) is an anti-nutritional factor of soybean, affecting cell proliferation and inducing cytotoxicity. Integrins are transmembrane receptors, mediating a variety of cell biological processes. This research aims to study the effects of SBA on cell proliferation and cell cycle progression of the intestinal epithelial cell line from piglets (IPEC-J2), to identify the integrin subunits especially expressed in IPEC-J2s, and to analyze the functions of these integrins on IPEC-J2 cell cycle progression and SBA-induced IPEC-J2 cell cycle alteration...
February 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28196413/alu-derived-alternative-splicing-events-specific-to-macaca-lineages-in-ctsf-gene
#20
Ja-Rang Lee, Sang-Je Park, Young-Hyun Kim, Se-Hee Choe, Hyeon-Mu Cho, Sang-Rae Lee, Sun-Uk Kim, Ji-Su Kim, Bo-Woong Sim, Bong-Seok Song, Kang-Jin Jeong, Youngjeon Lee, Yeung Bae Jin, Philyong Kang, Jae-Won Huh, Kyu-Tae Chang
Cathepsin F, which is encoded by CTSF, is a cysteine proteinase ubiquitously expressed in several tissues. In a previous study, novel transcripts of the CTSF gene were identified in the crab-eating monkey deriving from the integration of an Alu element-AluYRa1. The occurrence of AluYRa1-derived alternative transcripts and the mechanism of exonization events in the CTSF gene of human, rhesus monkey, and crab-eating monkey were investigated using PCR and reverse transcription PCR on the genomic DNA and cDNA isolated from several tissues...
February 2017: Molecules and Cells
journal
journal
32586
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"