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Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara

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https://www.readbyqxmd.com/read/29666561/comparison-of-barricor%C3%A2-vs-lithium-heparin-tubes-for-selected-routine-biochemical-analytes-and-evaluation-of-post-centrifugation-stability
#1
Anne Marie Dupuy, Stéphanie Badiou, Delphine Daubin, Anne Sophie Bargnoux, Chloé Magnan, Kadda Klouche, Jean Paul Cristol
Introduction: Obtaining suitable results unaffected by pre- or postanalytical phases is pivotal for clinical chemistry service. We aimed comparison and stability of nine biochemical analytes after centrifugation using Barricor™ plasma tubes with mechanical separator vs standard Vacutainer® lithium heparin tubes. Materials and methods: We collected samples on six healthy volunteers and nine patients from intensive care units into 6 mL plastic Vacutainer® lithium heparin tubes and 5...
June 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29666560/storage-stability-of-commonly-used-haematological-parameters-at-33-%C3%A2-c-electronic-supplementary-material-available-online-for-this-article
#2
Ashish Jain, Sanchit Jain, Neha Singh, Priyanka Aswal, Shweta Pal, Sushant Kumar Meinia, Nilotpal Chowdhury
Introduction: This study aimed to investigate the analytical bias in haematological parameters induced by storage at 33 ºC. Materials and methods: Blood from the diversion pouch of 20 blood donors were collected in K 2 EDTA vials and stored at 33 ºC. Readings from each vial were taken at 0, 4, 6, 12, 24, 48 and 72 hours after collection on the Sysmex XP-100 analyser (Sysmex Corporation, Kobe, Japan). The percent difference from the baseline readings were calculated and subjected to a Wilcoxon signed rank test at a Holm corrected significance level of 0...
June 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29666559/assessing-performance-in-pre-season-wrestling-athletes-using-biomarkers
#3
Ionas Papassotiriou, Artemissia-Phoebe Nifli
Introduction: Although regular training introduces the desired changes in athletes' metabolism towards optimal final performance, literature is rarely focusing on the metabolic responses off-competition. Therefore, the aim of this study was to evaluate biochemical indices during typical preseason training in wrestling athletes. Materials and methods: Twenty male freestyle and Greco-roman wrestlers (14 to 31 years) followed a typical session of the preparatory phase...
June 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29666558/verification-of-a-novel-point-of-care-hba-1c-device-in-real-world-clinical-practice-by-comparison-to-three-high-performance-liquid-chromatography-instruments
#4
Yufei Wang, Wei Peng, Junling Tang, Lu Dong, Chengchen Gu, Xin Zhang, Jian Zhou, Weiping Jia
Introduction: A real world clinical study was designed and conducted to evaluate the performance of a novel point-of-care device for determination of glycated haemoglobin A1c (HbA1c ), A1C EZ 2.0, in daily clinical practice. Materials and methods: Five hundred and fourteen subjects were included in this study, and divided into three groups. HbA1c was measured by A1C EZ 2.0 and three different high performance liquid chromatography (HPLC) devices: Bio-Rad Variant II Turbo, Tosoh HLC-723 G8 and Premier Hb9210 separately...
June 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29666557/the-impact-of-delayed-sample-handling-and-type-of-anticoagulant-on-the-interpretation-of-dysplastic-signs-detected-by-flow-cytometry
#5
Bettina Kárai, Zsófia Miltényi, Lajos Gergely, Marianna Száraz-Széles, János Kappelmayer, Zsuzsanna Hevessy
Introduction: A growing body of evidence supports the usefulness of dysplastic signs detected by flow cytometry in the diagnosis of myelodysplastic syndromes (MDS). Our aim was to assess the impact of pre-analytical variables (delayed sample handling, type of anticoagulant, and different clones of antibody) in the interpretation of flow cytometric results. Material and methods: Bone marrow samples were labelled and analysed immediately after aspiration and on two consecutive days...
June 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29666556/maturity-onset-diabetes-of-the-young-due-to-hnf1a-variants-in-croatia
#6
Tamara Pavić, Agata Juszczak, Edita Pape Medvidović, Carla Burrows, Mario Šekerija, Amanda J Bennett, Jadranka Ćuća Knežević, Anna L Gloyn, Gordan Lauc, Mark I McCarthy, Olga Gornik, Katharine R Owen
Introduction: Maturity onset diabetes of the young due to HNF1A mutations (HNF1A-MODY) is the most frequent form of monogenic diabetes in adults. It is often misdiagnosed as type 1 or type 2 diabetes, but establishing genetic diagnosis is important, as treatment differs from the common types of diabetes. HNF1A-MODY has not been investigated in Croatia before due to limited access to genetic testing. In this study we aimed to describe the characteristics of young adults diagnosed with diabetes before the age of 45 years, who have rare HNF1A allele variants, and estimate the prevalence of HNF1A-MODY in Croatia...
June 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29666555/national-survey-on-internal-quality-control-for-tumour-markers-in-clinical-laboratories-in-china
#7
Wei Wang, Kun Zhong, Shuai Yuan, Falin He, Yuxuan Du, Zhehui Hu, Zhiguo Wang
Introduction: This survey was initiated to obtain knowledge on the current situation of internal quality control (IQC) practice for tumour markers (TMs) in China. Additionally, we tried to acquire the most appropriate quality specifications. Materials and methods: This survey was a current status survey. The IQC information had been collected via online questionnaires. All of 1821 clinical laboratories which participated in the 2016 TMs external quality assessment (EQA) programme had been enrolled...
June 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29666554/effect-of-different-pre-analytical-conditions-on-plasma-lactate-concentration
#8
Ivana Rako, Ana Mlinaric, Monika Dozelencic, Gordana Fressl Juros, Dunja Rogic
Introduction: Plasma lactate is a frequently used and important parameter for medical decision making. To setup a pre-analytical algorithm, we aimed to investigate the influence of different test tube additives, aliquoting, ice storage and haemolysis on plasma lactate concentrations for possible sparing critically ill (ICU) patients of additional blood drawing. Materials and methods: In our study (N = 177), lactate concentration and haemolysis index (HI) were measured in aliquoted (AHP) and unaliquoted (HP) Li-heparin, NaF/K3 EDTA and NaF/KOX plasma, centrifuged within 15 minutes after venipuncture, on Cobas c501 analyzer...
June 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29666553/the-potential-of-component-resolved-diagnosis-in-laboratory-diagnostics-of-allergy
#9
REVIEW
Slavica Dodig, Ivana Čepelak
The initial laboratory approach in the diagnosis of allergies is to detect the type of allergic reaction, i.e. whether the patient's allergy is mediated by immunoglobulin E (IgE) or not. For this purpose, the concentration of total serum IgE (tIgE) and specific IgE (sIgE) are determined. Progress in laboratory diagnostics is the use of component-resolved diagnosis (CRD) which implies determination of sIgE against purified native and recombinant allergenic molecules. Component-resolved diagnosis is used in laboratory practice as singleplex and multiplex assays...
June 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29473636/corrigendum-to-nurses-knowledge-on-phlebotomy-in-tertiary-hospitals-in-china-a-cross-sectional-multicentric-survey
#10
Qian Cai, Yunxian Zhou, Dagan Yang
[This corrects the article DOI: 10.11613/BM.2018.010703.].
February 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29472808/pseudohyperkalemia-potassium-released-from-cells-due-to-clotting-and-centrifugation-a-case-report
#11
REVIEW
Tomáš Šálek
Hyperkalemia is a potentially lethal condition. Pseudohyperkalemia should be always excluded before implementing treatment to prevent inappropriate cause of hypokalemia - equally a potentially lethal condition. Here we present a case report of a 62 year female with chronic myeloproliferative disorder, i.e. essential thrombocythemia. The laboratory test results for potassium concentration were 6.3 mmol/L, for platelet count 1305 x109 /L and for leukocyte count 39.8 x109 /L. This was due to a temporary drug withdrawal after a surgical intervention for gastric bleeding...
February 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29472807/elevated-zinc-concentrations-in-a-5-months-old-infant-a-case-report
#12
REVIEW
Eva Rabing Brix Petersen, Sven Mortensen, Mads Nybo
Pre-analytical errors account for the majority of laboratory-associated errors. In a 5 months old infant hospitalised with lung dysfunction due to prematurity, a routine measurement of zinc revealed an unexpected elevated concentration of 20.2 µmol/L (reference interval 10.0 - 19.0 µmol/L) compared to 11.6 µmol/L five days earlier. Zinc measurement was repeated two days later and had further increased to 42.4 µmol/L. Of note, there were no clinical signs of the increased zinc concentrations. Performance data for the zinc analysis (performed by inductively coupled plasma mass spectrometry) was found satisfactory...
February 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29472806/neutrophil-gelatinase-associated-lipocalin-and-acute-kidney-injury-in-endovascular-aneurysm-repair-or-open-aortic-repair-a-pilot-study
#13
Benedetta Rampoldi, Serena Tessarolo, Paola Giubbilini, Paola Gaia, Samantha D Corino, Sarah Mazza, Roberta Rigolini, Marco Dei Poli, Elena Vianello, Massimiliano M Corsi Romanelli, Elena Costa
Introduction: Acute kidney injury (AKI) occurs frequently after abdominal aortic surgery and there is currently no effective marker able to detect early onset. The aim of this study is to evaluate the ability of neutrophil gelatinase-associated lipocalin (NGAL) to early identify the development of acute renal damage in patients undergoing endovascular aneurysm repair (EVAR) or open aortic repair (OAR). Materials and methods: Serial samples of blood and urine were obtained from 25 patients undergoing both EVAR and OAR...
February 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29472805/gamma-heavy-chain-disease-accompanied-with-follicular-lymphoma-a-case-report
#14
Paula San-José, Vicente Aguadero, Granada Perea, Meritxell Estrada, Eugenio Berlanga
Heavy chain diseases (HCD) are B-cell lymphoprolipherative disorders characterized by the production of monoclonal heavy chains without associated light chains. Some cases of gamma-HCD (γ-HCD) are concurrent with other lymphoid neoplasm. The monoclonal component is not always detectable by serum electrophoresis, and often an immunofixation procedure is necessary to detect this component. Prognosis is variable, and no established guidelines for follow-up are available. We describe a case of a challenging diagnosis of γ-HCD due to the absence of clinical signs frequently reported in the disease (anaemia and palatal oedema among others)...
February 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29472804/henoch-sch%C3%A3-nlein-purpura-in-the-third-trimester-of-pregnancy
#15
Ivka Djakovic, Drazan Butorac, Zeljko Vucicevic, Vesna Kosec, Andrea Tesija Kuna, Liborija Lugović-Mihić
Henoch-Schönlein purpura (HSP) is an IgA-mediated small vessels' vasculitis that affects the skin, intestines and kidneys. Pregnancy has been reported as an exacerbating factor. We present the case of a 24-year-old primigravida with HSP that occurred in the third trimester and lasted up to the end of the successful delivery. She had pruritic maculopapular exanthema on her legs. Biopsy of a cutaneous lesion was performed for histopathologic features and direct immunofluorescence (DIF) for the presence of perivascular IgA deposition...
February 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29472803/reference-interval-for-immature-platelet-fraction-on-sysmex-xn-haematology-analyser-in-adult-population
#16
Claudia E Imperiali, Ariadna Arbiol-Roca, Lourdes Sanchez-Navarro, Macarena Dastis-Arias, Juan C Lopez-Delgado, Anna Cortes-Bosch, Ana Sancho-Cerro, Dolors Dot-Bach
Introduction: The Sysmex XN-series haematology analyser has newly adopted a fluorescent channel to measure immature platelet fraction (IPF). To promote the clinical utility of this promising parameter, establishing a reliable reference interval is mandatory. According to previous studies, IPF values may be affected by the employed analyser and the ethnic background of the individual, but no differences seem to be found between individuals' genders. Therefore, this study aimed to define the reference interval for IPF in a Spanish population following Clinical and Laboratory Standard Institute (CLSI) guidelines...
February 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29472802/preanalytics-of-urine-sediment-examination-effect-of-relative-centrifugal-force-tube-type-volume-of-sample-and-supernatant-removal
#17
Amalija Bunjevac, Nora Nikolac Gabaj, Marijana Miler, Anita Horvat
Introduction: Laboratories often modify procedures recommended by the European Urinalysis Guidelines for urine sediment analysis. The aim of this study was to compare the recommended protocol with our routine laboratory procedure and to evaluate the possible impact of modifications in the relative centrifugal force, type of tube, method of supernatant aspiration and urine volume on patient's results. Material and methods: Firstly, relative centrifugal force was investigated using 20 pairs of samples examined after centrifugation at 400xg and 1358xg...
February 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29472801/diagnostic-value-of-tumour-markers-in-pleural-effusions
#18
Darian Volarić, Veljko Flego, Gordana Žauhar, Ljiljana Bulat-Kardum
Introduction: We investigated whether tumour markers carcinoembryonic antigen (CEA), neuron-specific enolase (NSE), cancer antigen 125 (CA-125), and cytokeratin 19 fragment (CYFRA 21-1) in pleural effusions and serum can be used to distinguish pleural effusion aetiology. Materials and methods: During the first thoracentesis, we measured pleural fluid and serum tumour marker concentrations and calculated the pleural fluid/serum ratio for patients diagnosed with pleural effusion, using electrochemiluminescence immunoassays...
February 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29472800/-biochemia-medica-appoints-new-editor-in-chief-and-new-senior-editor
#19
EDITORIAL
Daria Pašalić
No abstract text is available yet for this article.
February 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29472799/setting-the-impact-improve-access-to-clinical-trial-data-observatory-baseline
#20
REVIEW
Mersiha Mahmić-Kaknjo, Josip Šimić, Karmela Krleža-Jerić
Introduction: The aim of the IMPACT (IMProving Access to Clinical Trial data) Observatory is to assess the transformation of clinical trials (CT) related to the evolution of sharing of CT data. The objective of this study is to establish a baseline for monitoring CT data sharing by the Observatory. Materials and methods: In this scoping review we searched for publications that address sharing, dissemination, transparency or reuse of CT data published prior to December 31st 2000...
February 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
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