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Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara

journal
https://www.readbyqxmd.com/read/29209139/bayes-theorem-the-roc-diagram-and-reference-values-definition-and-use-in-clinical-diagnosis
#1
REVIEW
Anders Kallner
Medicine is diagnosis, treatment and care. To diagnose is to consider the probability of the cause of discomfort experienced by the patient. The physician may face many options and all decisions are liable to uncertainty to some extent. The rational action is to perform selected tests and thereby increase the pre-test probability to reach a superior post-test probability of a particular option. To draw the right conclusions from a test, certain background information about the performance of the test is necessary...
February 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29188823/corrigendum-to-minimum-requirements-for-the-estimation-of-the-measurement-uncertainty-recommendations-of-the-joint-working-group-for-uncertainty-of-measurement-of-the-csmblm-and-ccmb
#2
Ivana Ćelap, Ines Vukasović, Gordana Juričić, Ana-Maria Šimundić
[This corrects the article DOI: 10.11613/BM.2017.030502.].
February 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29187801/upfront-dilution-of-ferritin-samples-to-reduce-hook-effect-improve-turnaround-time-and-reduce-costs
#3
Shu Juan Wu, Joshua A Hayden
Introduction: Sandwich immunoassays offer advantages in the clinical laboratory but can yield erroneously low results due to hook (prozone) effect, especially with analytes whose concentrations span several orders of magnitude such as ferritin. This study investigated a new approach to reduce the likelihood of hook effect in ferritin immunoassays by performing upfront, five-fold dilutions of all samples for ferritin analysis. The impact of this change on turnaround time and costs were also investigated...
February 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29187800/reference-intervals-for-six-salivary-cortisol-measures-based-on-the-croatian-late-adolescence-stress-study-class
#4
Daniela Šupe-Domić, Goran Milas, Lada Stanišić, Irena Drmić Hofman, Irena Martinović Klarić
Introduction: The aim of this nested study is to provide the reference intervals for already published measurements of salivary cortisol from the Croatian Adolescence Stress Study (CLASS). Material and methods: A total of 969 individuals (372 males and 597 females) were included in the reference sample (age range: 18-21 years). Salivary cortisol concentrations were determined by the enzyme immunoassay (LUCIO-Medical ELISA Salivary Cortisol Kit, Nal von Minden, Germany) in the Department of Medical Laboratory Diagnostics, University Hospital Split...
February 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29187799/appropriateness-of-digoxin-measurement-in-hospitalized-patients
#5
Seyma Oncu, Ayse Gelal, Ozgur Aslan, Reyhan S Ucku
Introduction: Measurement of serum digoxin concentrations before steady-state is reached results in a falsely low concentration, and may affect treatment safety. We evaluated the proportion of serum digoxin measurements performed before steady-state is reached and the reasons for inappropriate sampling in hospitalized patients. Materials and methods: Electronic medical records of patients hospitalized between January 2011 and December 2015 treated with oral digoxin, that had more than one digoxin measurement were included...
February 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29187798/missed-detection-of-significant-positive-and-negative-shifts-in-gentamicin-assay-implications-for-routine-laboratory-quality-practices
#6
Gus Koerbin, Jiakai Liu, Alex Eigenstetter, Chin Hon Tan, Tony Badrick, Tze Ping Loh
Introduction: A product recall was issued for the Roche/Hitachi Cobas Gentamicin II assays on 25th May 2016 in Australia, after a 15 - 20% positive analytical shift was discovered. Laboratories were advised to employ the Thermo Fisher Gentamicin assay as an alternative. Following the reintroduction of the revised assay on 12th September 2016, a second reagent recall was made on 20th March 2017 after the discovery of a 20% negative analytical shift due to erroneous instrument adjustment factor...
February 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29187797/influence-of-centrifugation-conditions-on-the-results-of-77-routine-clinical-chemistry-analytes-using-standard-vacuum-blood-collection-tubes-and-the-new-bd-barricor-tubes
#7
Janne Cadamuro, Cornelia Mrazek, Alexander B Leichtle, Ulrike Kipman, Thomas K Felder, Helmut Wiedemann, Hannes Oberkofler, Georg M Fiedler, Elisabeth Haschke-Becher
Introduction: Although centrifugation is performed in almost every blood sample, recommendations on duration and g-force are heterogeneous and mostly based on expert opinions. In order to unify this step in a fully automated laboratory, we aimed to evaluate different centrifugation settings and their influence on the results of routine clinical chemistry analytes. Materials and methods: We collected blood from 41 healthy volunteers into BD Vacutainer PST II-heparin-gel- (LiHepGel), BD Vacutainer SST II-serum-, and BD Vacutainer Barricor heparin-tubes with a mechanical separator (LiHepBar)...
February 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29187796/nurses-knowledge-on-phlebotomy-in-tertiary-hospitals-in-china-a-cross-sectional-multicentric-survey
#8
Qian Cai, Yunxian Zhou, Dangan Yang
Introduction: In China, phlebotomy practice is mostly executed by nurses instead of phlebotomists. Our hypothesis was that these nurses may lack of knowledge on phlebotomy, especially factors influencing quality of blood samples. This study aims to assess the overall nurses' knowledge on phlebotomy to provide reference for improving blood sampling practice in China. Materials and methods: A survey was conducted involving nurses from 4 regions and 13 hospitals in China...
February 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29187795/fasting-conditions-influence-of-water-intake-on-clinical-chemistry-analytes
#9
Silvia F Benozzi, Gisela Unger, Amparo Campion, Graciela L Pennacchiotti
Introduction: Currently available recommendations regarding fasting requirements before phlebotomy do not specify any maximum water intake volume permitted during the fasting period. The aim was to study the effects of 300 mL water intake 1 h before phlebotomy on specific analytes. Materials and methods: Blood was collected from 20 women (median age (min-max): 24 (22 - 50) years) in basal state (T0) and 1 h after 300 mL water intake (T1). Glucose, total proteins (TP), urea, creatinine, cystatin C, total bilirubin (BT), total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triglycerides (Tg), uric acid (UA), high-sensitivity C-reactive protein, gamma-glutamyl transferase (GGT), aspartate-aminotransferase (AST), alanine-aminotransferase and lactate-dehydrogenase (LD) were studied...
February 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29187794/reliability-of-ckd-epi-predictive-equation-in-estimating-chronic-kidney-disease-prevalence-in-the-croatian-endemic-nephropathy-area
#10
Mirjana Fuček, Živka Dika, Sandra Karanović, Ivana Vuković Brinar, Vedran Premužić, Jelena Kos, Ante Cvitković, Maja Mišić, Josip Samardžić, Dunja Rogić, Bojan Jelaković
Introduction: Chronic kidney disease (CKD) is a significant public health problem and it is not possible to precisely predict its progression to terminal renal failure. According to current guidelines, CKD stages are classified based on the estimated glomerular filtration rate (eGFR) and albuminuria. Aims of this study were to determine the reliability of predictive equation in estimation of CKD prevalence in Croatian areas with endemic nephropathy (EN), compare the results with non-endemic areas, and to determine if the prevalence of CKD stages 3-5 was increased in subjects with EN...
February 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29180923/corrigendum-to-impact-of-reference-change-value-rcv-based-autoverification-on-turnaround-time-and-physician-satisfaction
#11
Esther Fernández-Grande, Carolina Varela-Rodriguez, Luis Sáenz-Mateos, Amparo Sastre-Gómez, Pilar García-Chico, Teodoro J Palomino-Muñoz
[This corrects the article DOI: 10.11613/BM.2017.037.].
October 15, 2017: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29180922/corrigendum-to-does-small-equal-predatory-analysis-of-publication-charges-and-transparency-of-editorial-policies-in-croatian-open-access-journals
#12
Jadranka Stojanovski, Ana Marušić
[This corrects the article DOI: 10.11613/BM.2017.032.].
October 15, 2017: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29180921/erratum-ethical-issues-in-publishing-in-predatory-journals
#13
Lorraine E Ferris, Margaret A Winker
[This corrects the article DOI: 10.11613/BM.2017.030.].
October 15, 2017: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29180920/urinary-free-cortisol-assessment-by-liquid-chromatography-tandem-mass-spectrometry-a-case-study-of-ion-suppression-due-to-unacquainted-administration-of-piperacillin
#14
REVIEW
Elisa Danese, Gian Luca Salvagno, Alessandra Guzzo, Samuele Scurati, Cristiano Fava, Giuseppe Lippi
Introduction: Liquid chromatography coupled to atmospheric pressure ionization tandem mass spectrometry (LC-ESI-MS/MS) is currently considered the reference method for quantitative determination of urinary free cortisol (UFC). One of the major drawbacks of this measurement is a particular form of matrix effect, conventionally known as ion suppression. Materials and methods: We describe here the case of a 66-year-old-patient referred to the daily service of general medicine for intravenous antibiotic administration due to a generalized Staphylococcus aureus infection and for routine 24 hours UFC monitoring in the setting of glucocorticoid replacement therapy...
October 15, 2017: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29180919/false-positive-immunoassay-for-heparin-induced-thrombocytopenia-in-the-presence-of-monoclonal-gammopathy-a-case-report
#15
Ivana Markovic, Zeljko Debeljak, Bojana Bosnjak, Maja Marijanovic
Heparin induced thrombocytopenia (HIT) is a life-threatening disorder which diagnosis depends on laboratory evaluation. The objective of this report is to present the impact of different laboratory methods for HIT detection on the diagnostic evaluation process. In this case, a 78-year old female patient previously diagnosed with monoclonal gammopathy of undetermined significance (MGUS) was administered with heparin for pulmonary embolism treatment. Patient's initial diagnostic work-up (determination of platelet count and prothrombin time measurement for monitoring of pharmacotherapy) was followed by the clinical estimation of HIT likelihood by "4Ts" score, two immunoassays (ID-PaGIA Heparin/PF4 Antibody Test and ELISA PF4 IgG assay) and one functional test called high-performance liquid chromatography serotonin release assay (HPLC-SRA)...
October 15, 2017: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29180918/analytical-evaluation-of-a-fully-automated-immunoassay-for-faecal-calprotectin-in-a-paediatric-setting
#16
Britta Noebauer, Lejla Ramic, Andrea Konstantin, Christina Zachbauer, Elisa Einwallner
Introduction: Faecal calprotectin (FC) is a routinely used marker for identifying and monitoring children with inflammatory bowel disease (IBD). This non-invasive test is useful for screening children with gastrointestinal symptoms to avoid unnecessary invasive procedures. In this study, we validated for the first time the performance of a fully automated particle-enhanced turbidimetric immunoassay (PETIA) on the VITROS® 5600 analyzer for measurement of FC in symptomatic children and adolescents...
October 15, 2017: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29180917/establishing-the-upper-reference-limit-of-galectin-3-in-healthy-blood-donors
#17
Luisa Agnello, Chiara Bellia, Bruna Lo Sasso, Alessia Pivetti, Maddalena Muratore, Concetta Scazzone, Giulia Bivona, Giuseppe Lippi, Marcello Ciaccio
Introduction: Galectin-3 (Gal-3) is an independent predictor of poor outcomes and mortality in patients with heart failure (HF). Thus, it has been proposed as a reliable prognostic biomarker for HF. The definition of reference intervals is mandatory for interpreting the findings of experimental studies and encouraging the routine use of biomarkers in clinical practice. To date, no study assessed the reference intervals of Gal-3 and identified the biological variables that affect its concentration in a well-defined healthy population...
October 15, 2017: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29180916/measurement-uncertainty-of-blood-ethanol-concentration-in-drink-driving-cases-in-an-emergency-laboratory
#18
Yasemin Ustundağ, Kağan Huysal
Introduction: The quality of blood ethanol concentration (BEC) determination is important because of its legal ramifications. Measurement uncertainty provides quantitative information about the quality and reliability of test results. In this study, we aim to calculate the measurement uncertainty for the ethanol test in our laboratory measured with a Synchron Systems Ethanol assay kit by employing an enzymatic rate method on the Beckman-Coulter Olympus AU400 auto analyzer (Beckman Coulter Inc, Melville, USA)...
October 15, 2017: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29180915/national-survey-on-current-situation-of-critical-value-reporting-in-973-laboratories-in-china
#19
Yang Fei, Haijian Zhao, Wei Wang, Falin He, Kun Zhong, Shuai Yuan, Zhiguo Wang
Introduction: The aim of the study was to investigate the state-of-the-art of the performance of critical value reporting and provide recommendations for laboratories setting critical value reporting time frames. Materials and methods: The National Centre for Clinical Laboratories in China initiated a critical value reporting investigation in 2015. A questionnaire related to critical value reporting policy was sent to 1589 clinical laboratories in China online. The questionnaire consisted of a set of questions related to critical value reporting policy and a set of questions related to timeliness of critical value reporting...
October 15, 2017: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29180914/insights-into-cerumen-and-application-in-diagnostics-past-present-and-future-prospective
#20
REVIEW
Engy Shokry, Nelson Roberto Antoniosi Filho
Cerumen or earwax is an emerging bio-fluid in clinical diagnosis that has been very little exploited during the past decades in spite of its high diagnostic potential. It is highly abundant in diagnostic biomarkers such as genetic material, lipids, proteins, chemical elements, internal and external metabolites (e.g. hormones, volatile organic compounds, amino acids, xenobiotics etc.) reaching earwax from the blood circulation. Thus, it is able to reflect not only physiology, pathophysiology of the human body but can also detect recent and long term exposure to environmental pollutants, without the need of invasive blood tests and in the same time overcoming many disadvantages faced by using other diagnostic biological fluids...
October 15, 2017: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
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