journal
MENU ▼
Read by QxMD icon Read
search

Experimental & Molecular Medicine

journal
https://www.readbyqxmd.com/read/29123254/implications-of-aging-and-the-endoplasmic-reticulum-unfolded-protein-response-on-the-molecular-modality-of-breast-cancer
#1
REVIEW
Rinki Minakshi, Safikur Rahman, Arif Tasleem Jan, Ayyagari Archana, Jihoe Kim
The endoplasmic reticulum (ER) is an important subcellular organelle that is involved in numerous activities required to achieve and maintain functional proteins in addition to its role in the biosynthesis of lipids and as a repository of intracellular Ca(2+). The inability of the ER to cope with protein folding beyond its capacity causes disturbances that evoke ER stress. Cells possess molecular mechanisms aimed at clearing unwanted cargo from the ER lumen as an adaptive response, but failing to do so navigates the system towards cell death...
November 10, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29099095/il-33-promotes-il-10-production-in-macrophages-a-role-for-il-33-in-macrophage-foam-cell-formation
#2
Hai-Feng Zhang, Mao-Xiong Wu, Yong-Qing Lin, Shuang-Lun Xie, Tu-Cheng Huang, Pin-Ming Liu, Ru-Qiong Nie, Qin-Qi Meng, Nian-Sang Luo, Yang-Xin Chen, Jing-Feng Wang
We evaluated the role of IL-10- in IL-33-mediated cholesterol reduction in macrophage-derived foam cells (MFCs) and the mechanism by which IL-33 upregulates IL-10. Serum IL-33 and IL-10 levels in coronary artery disease patients were measured. The effects of IL-33 on intra-MFC cholesterol level, IL-10, ABCA1 and CD36 expression, ERK 1/2, Sp1, STAT3 and STAT4 activation, and IL-10 promoter activity were determined. Core sequences were identified using bioinformatic analysis and site-specific mutagenesis. The serum IL-33 levels positively correlated with those of IL-10...
November 3, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29075038/20-s-protopanaxadiol-enhances-angiogenesis-via-hif-1%C3%AE-mediated-vegf-secretion-by-activating-p70s6-kinase-and-benefits-wound-healing-in-genetically-diabetic-mice
#3
Er-Yun Zhang, Bo Gao, Hai-Lian Shi, Ling-Fang Huang, Li Yang, Xiao-Jun Wu, Zheng-Tao Wang
Impaired angiogenesis is one of the crucial factors that impede the wound healing process in diabetic foot ulcers (DFUs). In this study, we found that 20(S)-protopanaxadiol (PPD), an aglycone of ginsenosides in Panax notoginseng, stimulated angiogenesis and benefited wound healing in genetically diabetic mice. In HUVECs, PPD promoted cell proliferation, tube formation and VEGF secretion accompanied by increased nuclear translocalization of HIF-1α, which led to elevated VEGF mRNA expression. PPD activated both PI3K/Akt/mTOR and Raf/MEK/ERK signaling pathways in HUVECs, which were abrogated by LY294002 and PD98059...
October 27, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29053138/mir-31a-5p-promotes-postnatal-cardiomyocyte-proliferation-by-targeting-rhobtb1
#4
Junjie Xiao, Hui Liu, Dragos Cretoiu, Daniela Oana Toader, Nicolae Suciu, Jing Shi, Shutong Shen, Yihua Bei, Joost Pg Sluijter, Saumya Das, Xiangqing Kong, Xinli Li
A limited number of microRNAs (miRNAs, miRs) have been reported to control postnatal cardiomyocyte proliferation, but their strong regulatory effects suggest a possible therapeutic approach to stimulate regenerative capacity in the diseased myocardium. This study aimed to investigate the miRNAs responsible for postnatal cardiomyocyte proliferation and their downstream targets. Here, we compared miRNA profiles in cardiomyocytes between postnatal day 0 (P0) and day 10 (P10) using miRNA arrays, and found that 21 miRNAs were upregulated at P10, whereas 11 were downregulated...
October 20, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29026198/ctbp2-mediated-%C3%AE-catenin-regulation-is-required-for-exit-from-pluripotency
#5
Tae Wan Kim, Sojung Kwak, Jihoon Shin, Byung-Hee Kang, Sang-Eun Lee, Min Young Suh, Jae-Hwan Kim, In-Young Hwang, Jong-Hyuk Lee, Jinmi Choi, Eun-Jung Cho, Hong-Duk Youn
The canonical Wnt pathway is critical for embryonic stem cell (ESC) pluripotency and aberrant control of β-catenin leads to failure of exit from pluripotency and lineage commitments. Hence, maintaining the appropriate level of β-catenin is important for the decision to commit to the appropriate lineage. However, how β-catenin links to core transcription factors in ESCs remains elusive. C-terminal-binding protein (CtBP) in Drosophila is essential for Wnt-mediated target gene expression. In addition, Ctbp acts as an antagonist of β-catenin/TCF activation in mammals...
October 13, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28983090/muscle-fatigue-general-understanding-and-treatment
#6
REVIEW
Jing-Jing Wan, Zhen Qin, Peng-Yuan Wang, Yang Sun, Xia Liu
Muscle fatigue is a common complaint in clinical practice. In humans, muscle fatigue can be defined as exercise-induced decrease in the ability to produce force. Here, to provide a general understanding and describe potential therapies for muscle fatigue, we summarize studies on muscle fatigue, including topics such as the sequence of events observed during force production, in vivo fatigue-site evaluation techniques, diagnostic markers and non-specific but effective treatments.
October 6, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28960214/upregulation-of-fc%C3%AE-riib-by-resveratrol-via-nf-%C3%AE%C2%BAb-activation-reduces-b-cell-numbers-and-ameliorates-lupus
#7
Jyun-Pei Jhou, Se-Jie Chen, Ho-Yin Huang, Wan-Wan Lin, Duen-Yi Huang, Shiang-Jong Tzeng
Resveratrol, an anti-inflammatory agent, can inhibit pro-inflammatory mediators by activating Sirt1, which is a class III histone deacetylase. However, whether resveratrol can regulate inhibitory or anti-inflammatory molecules has been less studied. FcγRIIB, a receptor for IgG, is an essential inhibitory receptor of B cells for blocking B-cell receptor-mediated activation and for directly inducing apoptosis of B cells. Because mice deficient in either Sirt1 or FcγRIIB develop lupus-like diseases, we investigated whether resveratrol can alleviate lupus through FcγRIIB...
September 29, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28960213/impaired-polyfunctionality-of-cd8-t-cells-in-severe-sepsis-patients-with-human-cytomegalovirus-reactivation
#8
Young Joon Choi, Sun Bean Kim, Jong Hoon Kim, Su-Hyung Park, Moo Suk Park, June Myung Kim, Sang Hoon Han, Eui-Cheol Shin
Human cytomegalovirus (HCMV) establishes a lifelong chronic latent infection and often reactivates in immunocompromised patients. In addition, HCMV reactivates in patients with sepsis or other critical illnesses, particularly in patients with poor prognoses. However, the immunological characteristics of sepsis patients with HCMV reactivation have not been elucidated. In the present study, we examined T-cell responses in severe sepsis patients with and without HCMV reactivation. First, HCMV pp65-specific T-cell functions were assessed by intracellular cytokine staining (ICS) for IFN-γ, TNF-α, and MIP-1β and by CD107a staining...
September 29, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28960212/a-network-meta-analysis-of-the-short-term-efficacy-of-five-chemotherapy-regimens-based-on-cisplatin-and-fluorouracil-for-esophagogastric-junctional-adenocarcinoma
#9
Cong Wang, Dong-Jian Song, Zhi-Li Xu, Shu-Ping Xie, Jun-Hong Hu
The primary purpose of this study was to explore the short-term efficacy of different cisplatin and fluorouracil-based chemotherapy regimens in the treatment of patients with esophagogastric junctional adenocarcinoma (EGJA) using a network meta-analysis (NMA). Randomized controlled trials (RCTs) related to chemotherapy regimens based on cisplatin and fluorouracil for EGJA were included from the PubMed, EMBASE and Cochrane Library electronic databases (from inception to June 2016). Direct and indirect evidence were combined to calculate the pooled odds ratio (OR) and its 95% confidence interval (95% CI) as well as to draw the surface under the cumulative ranking (SUCRA) curves...
September 29, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28935976/tgf-%C3%AE-1-suppresses-ccl3-4-expression-through-the-erk-signaling-pathway-and-inhibits-intervertebral-disc-degeneration-and-inflammation-related-pain-in-a-rat-model
#10
Jian Zhang, Zemin Li, Fan Chen, Hui Liu, Hua Wang, Xiang Li, Xianguo Liu, Jianru Wang, Zhaomin Zheng
The objective of this study was to investigate the regulatory effects of TGF-β1 on CCL3/4 expression and inflammation-related pain during intervertebral disc degeneration (IVDD). TGF-β1 and CCL3/4 expression patterns in different degenerative human nucleus pulposus (NP) tissues were measured by qPCR and immunohistochemistry (IHC), and the effects of TGF-β1 on CCL3/4 expression were measured by qPCR, ELISA and immunofluorescence. The roles of NF-κB and MAPK in TGF-β1-mediated CCL3/4 promoter activity were studied using siRNAs, western blotting and qPCR...
September 22, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28935975/tumor-necrosis-factor-inducible-gene-6-protein-ameliorates-chronic-liver-damage-by-promoting-autophagy-formation-in-mice
#11
Sihyung Wang, Chanbin Lee, Jieun Kim, Jeongeun Hyun, Minso Lim, Hyuk-Jin Cha, Seh-Hoon Oh, Yung Hyun Choi, Youngmi Jung
Tumor necrosis factor-inducible gene 6 protein (TSG-6) has recently been shown to protect the liver from acute damage. However, the mechanism underlying the effect of TSG-6 on the liver remains unclear. Autophagy is a catabolic process that targets cell components to lysosomes for degradation, and its functions are reported to be dysregulated in liver diseases. Here we investigate whether TSG-6 promotes liver regeneration by inducing autophagic clearance in damaged livers. Mice fed a methionine choline-deficient diet supplemented with 0...
September 22, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28912572/follistatin-n-terminus-differentially-regulates-muscle-size-and-fat-in-vivo
#12
Hui Zheng, Chunping Qiao, Ruhang Tang, Jianbin Li, Karen Bulaklak, Zhenhua Huang, Chunxia Zhao, Yi Dai, Juan Li, Xiao Xiao
Delivery of follistatin (FST) represents a promising strategy for both muscular dystrophies and diabetes, as FST is a robust antagonist of myostatin and activin, which are critical regulators of skeletal muscle and adipose tissues. FST is a multi-domain protein, and deciphering the function of different domains will facilitate novel designs for FST-based therapy. Our study aims to investigate the role of the N-terminal domain (ND) of FST in regulating muscle and fat mass in vivo. Different FST constructs were created and packaged into the adeno-associated viral vector (AAV)...
September 15, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28912571/comparative-global-immune-related-gene-profiling-of-somatic-cells-human-pluripotent-stem-cells-and-their-derivatives-implication-for-human-lymphocyte-proliferation
#13
Chia-Eng Wu, Chen-Wei Yu, Kai-Wei Chang, Wen-Hsi Chou, Chen-Yu Lu, Elisa Ghelfi, Fang-Chun Wu, Pey-Shynan Jan, Mei-Chi Huang, Patrick Allard, Shau-Ping Lin, Hong-Nerng Ho, Hsin-Fu Chen
Human pluripotent stem cells (hPSCs), including embryonic stem cells (ESCs) and induced PSCs (iPSCs), represent potentially unlimited cell sources for clinical applications. Previous studies have suggested that hPSCs may benefit from immune privilege and limited immunogenicity, as reflected by the reduced expression of major histocompatibility complex class-related molecules. Here we investigated the global immune-related gene expression profiles of human ESCs, hiPSCs and somatic cells and identified candidate immune-related genes that may alter their immunogenicity...
September 15, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28912570/a-focus-on-extracellular-ca-2-entry-into-skeletal-muscle
#14
REVIEW
Chung-Hyun Cho, Jin Seok Woo, Claudio F Perez, Eun Hui Lee
The main task of skeletal muscle is contraction and relaxation for body movement and posture maintenance. During contraction and relaxation, Ca(2+) in the cytosol has a critical role in activating and deactivating a series of contractile proteins. In skeletal muscle, the cytosolic Ca(2+) level is mainly determined by Ca(2+) movements between the cytosol and the sarcoplasmic reticulum. The importance of Ca(2+) entry from extracellular spaces to the cytosol has gained significant attention over the past decade...
September 15, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28883547/casein-kinase-2-is-a-critical-determinant-of-the-balance-of-th17-and-treg-cell-differentiation
#15
Sung Woong Jang, Soo Seok Hwang, Hyeong Su Kim, Keoung Oh Lee, Min Kyung Kim, Wonyong Lee, Kiwan Kim, Gap Ryol Lee
Th17 cells promote inflammatory reactions, whereas regulatory T (Treg) cells inhibit them. Thus, the Th17/Treg cell balance is critically important in inflammatory diseases. However, the molecular mechanisms underlying this balance are unclear. Here, we demonstrate that casein kinase 2 (CK2) is a critical determinant of the Th17/Treg cell balance. Both the inhibition of CK2 with a specific pharmacological inhibitor, CX-4945, and its small hairpin RNA (shRNA)-mediated knockdown suppressed Th17 cell differentiation but reciprocally induced Treg cell differentiation in vitro...
September 8, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28883546/api5-induces-cisplatin-resistance-through-fgfr-signaling-in-human-cancer-cells
#16
Han Sol Jang, Seon Rang Woo, Kwon-Ho Song, Hanbyoul Cho, Doo Byung Chay, Soon-Oh Hong, Hyo-Jung Lee, Se Jin Oh, Joon-Yong Chung, Jae-Hoon Kim, Tae Woo Kim
Most tumors frequently undergo initial treatment with a chemotherapeutic agent but ultimately develop resistance, which limits the success of chemotherapies. As cisplatin exerts a high therapeutic effect in a variety of cancer types, it is often used in diverse strategies, such as neoadjuvant, adjuvant and combination chemotherapies. However, cisplatin resistance has often manifested regardless of cancer type, and it represents an unmet clinical need. Since we found that API5 expression was positively correlated with chemotherapy resistance in several specimens from patients with cervical cancer, we decided to investigate whether API5 is involved in the development of resistance after chemotherapy and to explore whether targeting API5 or its downstream effectors can reverse chemo-resistance...
September 8, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28860664/cadmium-induced-er-stress-and-inflammation-are-mediated-through-c-ebp-ddit3-signaling-in-human-bronchial-epithelial-cells
#17
Jeeyoung Kim, Haengseok Song, Hye-Ryeon Heo, Jung Woon Kim, Hye-Ryun Kim, Yoonki Hong, Se-Ran Yang, Seon-Sook Han, Seung-Joon Lee, Woo Jin Kim, Seok-Ho Hong
Cadmium (Cd), a major component of cigarette smoke, disrupts the normal functions of airway cells and can lead to the development of various pulmonary diseases such as chronic obstructive pulmonary disease (COPD). However, the molecular mechanisms involved in Cd-induced pulmonary diseases are poorly understood. Here, we identified a cluster of genes that are altered in response to Cd exposure in human bronchial epithelial cells (BEAS-2B) and demonstrated that Cd-induced ER stress and inflammation are mediated via CCAAT-enhancer-binding proteins (C/EBP)-DNA-damaged-inducible transcript 3 (DDIT3) signaling in BEAS-2B cells...
September 1, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28860663/bacterial-flagellin-a-potent-immunomodulatory-agent
#18
REVIEW
Irshad A Hajam, Pervaiz A Dar, Imam Shahnawaz, Juan Carlos Jaume, John Hwa Lee
Flagellin is a subunit protein of the flagellum, a whip-like appendage that enables bacterial motility. Traditionally, flagellin was viewed as a virulence factor that contributes to the adhesion and invasion of host cells, but now it has emerged as a potent immune activator, shaping both the innate and adaptive arms of immunity during microbial infections. In this review, we summarize our understanding of bacterial flagellin and host immune system interactions and the role flagellin as an adjuvant, anti-tumor and radioprotective agent, and we address important areas of future research interests...
September 1, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28857086/ccctc-binding-factor-is-essential-to-the-maintenance-and-quiescence-of-hematopoietic-stem-cells-in-mice
#19
Tae-Gyun Kim, Sueun Kim, Soyeon Jung, Mikyoung Kim, Bobae Yang, Min-Geol Lee, Hyoung-Pyo Kim
Hematopoiesis involves a series of lineage differentiation programs initiated in hematopoietic stem cells (HSCs) found in bone marrow (BM). To ensure lifelong hematopoiesis, various molecular mechanisms are needed to maintain the HSC pool. CCCTC-binding factor (CTCF) is a DNA-binding, zinc-finger protein that regulates the expression of its target gene by organizing higher order chromatin structures. Currently, the role of CTCF in controlling HSC homeostasis is unknown. Using a tamoxifen-inducible CTCF conditional knockout mouse system, we aimed to determine whether CTCF regulates the homeostatic maintenance of HSCs...
August 25, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28857085/enteric-dysbiosis-linked-gut-barrier-disruption-triggers-early-renal-injury-induced-by-chronic-high-salt-feeding-in-mice
#20
Jingjuan Hu, Haihua Luo, Jieyan Wang, Wenli Tang, Junqi Lu, Shan Wu, Zhi Xiong, Guizhi Yang, Zhenguo Chen, Tian Lan, Hongwei Zhou, Jing Nie, Yong Jiang, Peng Chen
Chronic high-salt diet-associated renal injury is a key risk factor for the development of hypertension. However, the mechanism by which salt triggers kidney damage is poorly understood. Our study investigated how high salt (HS) intake triggers early renal injury by considering the 'gut-kidney axis'. We fed mice 2% NaCl in drinking water continuously for 8 weeks to induce early renal injury. We found that the 'quantitative' and 'qualitative' levels of the intestinal microflora were significantly altered after chronic HS feeding, which indicated the occurrence of enteric dysbiosis...
August 25, 2017: Experimental & Molecular Medicine
journal
journal
32545
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"