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Cellular & Molecular Biology Letters

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https://www.readbyqxmd.com/read/30008754/lamp3-plays-an-oncogenic-role-in-osteosarcoma-cells-partially-by-inhibiting-tp53
#1
Shaoxian Liu, Junyi Yue, Wei Du, Jian Han, Weidong Zhang
Background: Osteosarcoma (OS) is a common malignant tumor that predominantly occurs in adolescents. Its most common metastasis is to the lungs. As shown in our earlier study, lysosome-associated membrane glycoprotein 3 (LAMP3) is highly upregulated in metastatic OS. However, its role in the regulation of OS cell viability and apoptosis remains unknown. Methods: We knocked down and overexpressed LAMP3 in OS cells and assessed the cell viability and apoptosis. Then, we investigated the expression of apoptosis-associated genes to identify the downstream gene(s) of LAMP3 ...
2018: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/30002683/laminopathies-what-can-humans-learn-from-fruit-flies
#2
REVIEW
Marta Pałka, Aleksandra Tomczak, Katarzyna Grabowska, Magdalena Machowska, Katarzyna Piekarowicz, Dorota Rzepecka, Ryszard Rzepecki
Lamin proteins are type V intermediate filament proteins (IFs) located inside the cell nucleus. They are evolutionarily conserved and have similar domain organization and properties to cytoplasmic IFs. Lamins provide a skeletal network for chromatin, the nuclear envelope, nuclear pore complexes and the entire nucleus. They are also responsible for proper connections between the karyoskeleton and structural elements in the cytoplasm: actin and the microtubule and cytoplasmic IF networks. Lamins affect transcription and splicing either directly or indirectly...
2018: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/30002682/dspp-mmp20-gene-silencing-downregulates-cancer-stem-cell-markers-in-human-oral-cancer-cells
#3
Nikolaos G Nikitakis, Ioannis Gkouveris, Jaya Aseervatham, Kelvin Barahona, Kalu U E Ogbureke
Background: Recent findings indicate that dentin sialophosphoprotein (DSPP) and matrix metalloproteinase (MMP) 20 interact in oral squamous cell carcinoma (OSCC). The objective of this study was to determine the effects of DSPP/MMP20 gene silencing on oral cancer stem cell (OCSC) markers. Methods: The expression of well-established OCSC markers: ABCG2; ALDH1; CD133; CD44; BMI1; LGR4, and Podoplanin in DSPP/MMP20-silenced OSCC cell line, OSC2, and controls were assayed by western blot (WB), and flow cytometry techniques...
2018: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29988653/genetic-variation-and-relationships-in-the-mitochondrial-dna-d-loop-region-of-qinghai-indigenous-and-commercial-pig-breeds
#4
Junxia Zhang, Baochun Yang, Xiaocheng Wen, Guoqiang Sun
Background: Bamei pigs are special germplasm resources in Qinghai. In the specific environment of the Qinghai plateau, local breeds, through long-term natural and artificial selection, have acquired a relatively stable population structure and genetic diversity. This study revealed Bamei pigs' genetic diversity at the molecular level and the degree of introgression of commercial breeds into Bamei pigs. Methods: The mitochondrial DNA D-loop region was amplified and sequenced using the ABI 3130 DNA sequencer...
2018: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29988306/clc-2-knockdown-prevents-cerebrovascular-remodeling-via-inhibition-of-the-wnt-%C3%AE-catenin-signaling-pathway
#5
Jingjing Lu, Feng Xu, Yingna Zhang, Hong Lu, Jiewen Zhang
Background: Mishandling of intracellular chloride (Cl- ) concentration ([Cl- ]i ) in cerebrovascular smooth muscle cells is implicated in several pathological processes, including hyperplasia and remodeling. We investigated the effects of ClC-2-mediated Cl- efflux on the proliferation of human brain vascular smooth muscle cells (HBVSMCs) induced by angiotensin II (AngII). Methods: Cell proliferation and motility were determined using the CCK-8, bromodeoxyuridine staining, wound healing and invasion assays...
2018: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29946339/microrna-488-inhibits-tongue-squamous-carcinoma-cell-invasion-and-emt-by-directly-targeting-atf3
#6
Bingxia Shi, Wei Yan, Guolin Liu, Yanjun Guo
Background: It has been reported that the expression of activating transcription factor 3 (ATF3) is closely associated with both microRNA (miRNA) processing and the progress of many cancers. Our study aimed to explore the interaction between ATF3 and miR-488 in tongue squamous cell carcinoma (TSCC). Methods: Quantitative real-time PCR was performed to detect the levels of ATF3 and miR-488 in TSCC tissues and cell lines. Cell invasion and epithelial-mesenchymal transition (EMT) were assessed to determine the biological functions of miR-488 and ATF3 in TSCC cells...
2018: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29946338/measuring-the-lactate-to-creatine-ratio-via-1-h-nmr-spectroscopy-can-be-used-to-noninvasively-evaluate-apoptosis-in-glioma-cells-after-x-ray-irradiation
#7
Hongxia Li, Yi Cui, Fuyan Li, Wenqi Shi, Wenjing Gao, Xiao Wang, Qingshi Zeng
Background: Radiotherapy is among the commonly applied treatment options for glioma, which is one of the most common types of primary brain tumor. To evaluate the effect of radiotherapy noninvasively, it is vital for oncologists to monitor the effects of X-ray irradiation on glioma cells. Preliminary research had showed that PKC-ι expression correlates with tumor cell apoptosis induced by X-ray irradiation. It is also believed that the lactate-to-creatine (Lac/Cr) ratio can be used as a biomarker to evaluate apoptosis in glioma cells after X-ray irradiation...
2018: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29881400/hif-1%C3%AE-promotes-the-migration-and-invasion-of-hepatocellular-carcinoma-cells-via-the-il-8-nf-%C3%AE%C2%BAb-axis
#8
Wenming Feng, Tao Xue, Sanxiong Huang, Qilin Shi, Chengwu Tang, Ge Cui, Guanghui Yang, Hui Gong, Huihui Guo
Background: Hypoxia plays a critical role in many cancers. Hypoxia inducible factor-1α (HIF-1α) is an important mediator of the hypoxia response. It regulates the expression of various chemokines involved in tumor growth, angiogenesis and metastasis but the associated pathway needs further investigation. Methods: The expression level of HIF-1α was determined in hepatocellular carcinoma (HCC) cells. The correlation of interleukin-8 (IL-8) and HIF-1α was assessed by knocking down HIF-1α...
2018: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29849669/essential-genes-of-the-macrophage-response-to-staphylococcus-aureus-exposure
#9
Aixia Sun, Hongwei Zhang, Feng Pang, Guifen Niu, Jianzhong Chen, Fei Chen, Jian Zhang
Background: Although significant advances have been made in understanding the mechanisms of macrophage response to Staphylococcus aureus infection, the molecular details are still elusive. Identification of the essential genes and biological processes of macrophages that are specifically changed at different durations of S. aureus exposure is of great clinical significance. Methods: We aimed to identify the significantly changed genes and biological processes of S...
2018: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29785186/platelets-activated-by-the-anti-%C3%AE-2gpi-%C3%AE-2gpi-complex-release-micrornas-to-inhibit-migration-and-tube-formation-of-human-umbilical-vein-endothelial-cells
#10
Yanfen Zhang, Wenjing Zhang, Caijun Zha, Yanhong Liu
Background: Patients with anti-β2GPI antibodies display significantly higher platelet activation/aggregation and vascular endothelial cell damage. The mechanism underlying the correlation between platelet activation, vascular endothelial cell dysfunctions and anti-β2GPI antibodies remains unknown. Methods: In this study, we derived miR-96 and -26a from platelets activated by the anti-β2GPI/β2GPI complex and explored their role in modulating human umbilical vein endothelial cell (HUVEC) migration and tube formation...
2018: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29760746/microrna-495-inhibits-the-high-glucose-induced-inflammation-differentiation-and-extracellular-matrix-accumulation-of-cardiac-fibroblasts-through-downregulation-of-nod1
#11
Xiaowei Wang, Haiying Jin, Shifeng Jiang, Yanlan Xu
Background: MicroRNAs (miRNAs) have physiological and pathophysiological functions that are involved in the regulation of cardiac fibrosis. This study aimed to investigate the effects of miR-495 on high glucose-induced cardiac fibrosis in human cardiac fibroblasts (CFs) and to establish the mechanism underlying these effects. Methods: Human CFs were transfected with an miR-495 inhibitor or mimic and incubated with high glucose. The levels of NOD1 and miR-495 were then determined via quantitative RT-PCR...
2018: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29760745/diagnostic-value-of-decoy-receptor-3-combined-with-procalcitonin-and-soluble-urokinase-type-plasminogen-activator-receptor-for-sepsis
#12
Jing-Jing Zhao, Xiao-Li Lou, Hong-Wei Chen, Feng-Ting Zhu, Yan-Qiang Hou
The levels of decoy receptor 3 (DcR3), soluble urokinase type plasminogen activator receptor (suPAR) and procalcitonin (PCT) are significantly increased in sepsis. We investigated the diagnostic value of DcR3 combined with suPAR and PCT in sepsis. Patients with sepsis, non-infectious systemic inflammatory response comprehensive syndrome (SIRS) and healthy controls were recruited according to the diagnostic standard. We measured DcR3, suPAR, PCT, interleukin-6 (IL-6) and C-reactive protein (CRP), and the diagnostic value was evaluated by receiver operating characteristics (ROC) curves...
2018: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29760744/mst1-regulates-post-infarction-cardiac-injury-through-the-jnk-drp1-mitochondrial-fission-pathway
#13
Xisong Wang, Qing Song
Background: Post-infarction cardiac injury is closely associated with cardiac remodeling and heart dysfunction. Mammalian STE20-like kinase 1 (Mst1), a regulator of cellular apoptosis, is involved in cardiac remodeling in post-infarction heart, but the mechanisms remain poorly defined. We aimed to explore the role of Mst1 in regulating chronic post-infarction cardiac injury, with a focus on mitochondrial homoeostasis. Methods: Wild-type (WT) and Mst1-knockout mice were as the cardiac myocardial infarction model...
2018: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29760743/crosstalk-between-the-warburg-effect-redox-regulation-and-autophagy-induction-in-tumourigenesis
#14
REVIEW
Mokgadi Violet Gwangwa, Anna Margaretha Joubert, Michelle Helen Visagie
Tumourigenic tissue uses modified metabolic signalling pathways in order to support hyperproliferation and survival. Cancer-associated aerobic glycolysis resulting in lactic acid production was described nearly 100 years ago. Furthermore, increased reactive oxygen species (ROS) and lactate quantities increase metabolic, survival and proliferation signalling, resulting in increased tumourigenesis. In order to maintain redox balance, the cell possesses innate antioxidant defence systems such as superoxide dismutase, catalase and glutathione...
2018: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29743895/osthole-improves-collagen-induced-arthritis-in-a-rat-model-through-inhibiting-inflammation-and-cellular-stress
#15
Renguo Xu, Zhen Liu, Jiande Hou, Tao Huang, Ming Yang
Background: Osthole is a natural product that has multiple bioactive functions and has been reported to exert potent immunosuppressive effects. However, the therapeutic effect of osthole on arthritis has not been explored. In the present study, a collagen-induced arthritis rat model, IL-1β-stimulated SW982 cells, and RA-like fibroblast-like synoviocytes (FLS) were employed to investigate the effect and possible mechanism of osthole on arthritis in vivo and in vitro. Results: 20 and 40 mg/kg osthole significantly alleviated collagen-induced arthritic symptoms based on histopathology and clinical arthritis scores, and improved erosion using HE staining...
2018: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29743894/lycium-barbarum-polysaccharide-protects-hsf-cells-against-ultraviolet-induced-damage-through-the-activation-of-nrf2
#16
Bihua Liang, Liqian Peng, Runxiang Li, Huaping Li, Ziyin Mo, Xinyue Dai, Na Jiang, Qing Liu, Erting Zhang, Huiyan Deng, Zhenjie Li, Huilan Zhu
Background: Lycium barbarum polysaccharide (LBP) is considered an antioxidant agent. NF-E2-related factor-2 (Nrf2) is an important regulator for protection against UV damage. In this study, we verified the performance of LBP and the correlation between LBP and Nrf2. Methods: HSF cells were treated with LBP to determine dose and time dependencies. An antioxidant response element (ARE) reporter was designed to monitor the activity of the Nrf2 antioxidant pathway. Results: For HSF cells, the optimal LBP treatment was 300 μg/ml for 3 h...
2018: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29736176/knockdown-of-the-lncrna-snhg8-inhibits-cell-growth-in-epstein-barr-virus-associated-gastric-carcinoma
#17
Jing Liu, Chunxia Yang, Yufang Gu, Chong Li, Huamei Zhang, Wenfang Zhang, Xueqing Wang, Nan Wu, Chunyan Zheng
Background: Epstein-Barr virus (EBV) infection is causatively associated with a variety of human cancers, including gastric cancer (GC), which has one of the highest mortality rates of all human cancers. Long non-coding RNAs (lncRNAs) show important regulatory roles in human GC. SNHG8 is a recently identified lncRNA that was reported to show abnormal expression pattern in GC. However, little is known of its biological function in EBV-associated GC. Methods: We used cell viability, colony formation and cell cycle assays to investigate the roles of lncRNA SNHG8 in the cell growth of EBV-associated GC...
2018: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29721023/mirna-17-encoded-by-the-mir-17-92-cluster-increases-the-potential-for-steatosis-in-hepatoma-cells-by-targeting-cyp7a1
#18
Ruijie Gong, Xiaofei Lv, Fengqiong Liu
Background: The miRNA cluster miR-17-92 is known to act as an oncogene in various cancers. Members of this cluster were also found to be involved in some other pathological process, such as steatosis, which is a pivotal event in the initiation and progression of nonalcoholic fatty liver disease (NAFLD). This study aimed to explore whether miR-17, one of the most functional miRNAs in the miR-17-92 family, participates in the process of steatosis in hepatoma cells. Methods: We developed both a miR-17-expressing transgenic mouse model and a miR-17-expressing HepG2 cell model, the latter was established via stable transfection...
2018: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29713353/the-norpurpureine-alkaloid-from-annona-purpurea-inhibits-human-platelet-activation-in-vitro
#19
Gabriela Sánchez, Omar Estrada, Giovana Acha, Alfonso Cardozo, Franshelle Peña, Marie Christine Ruiz, Fabián Michelangeli, Claudia Alvarado-Castillo
Background: The leaves of Annona purpurea have yielded several alkaloids with anti-aggregation activities against rabbit platelets. This is promising in the search for agents that might act against platelets and reduce the incidence of cardiovascular diseases. Since significant differences in platelet function have been reported between human and animal platelets, a study focusing on the effect of A. purpurea extracts against human platelet activation is necessary. Methods: The compounds in an A...
2018: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29636771/mitochonic-acid-5-activates-the-mapk-erk-yap-signaling-pathways-to-protect-mouse-microglial-bv-2-cells-against-tnf%C3%AE-induced-apoptosis-via-increased-bnip3-related-mitophagy
#20
Qingyun Lei, Jian Tan, Shangqing Yi, Na Wu, Yilin Wang, Heng Wu
Background: The regulation of microglial function via mitochondrial homeostasis is important in the development of neuroinflammation. The underlying mechanism for this regulatory function remains unclear. In this study, we investigated the protective role of mitochonic acid 5 (MA-5) in microglial mitochondrial apoptosis following TNFα-induced inflammatory injury. Methods: TNFα was used to induce inflammatory injury in mouse microglial BV-2 cells with and without prior MA-5 treatment...
2018: Cellular & Molecular Biology Letters
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