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Cellular & Molecular Biology Letters

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https://www.readbyqxmd.com/read/28932249/the-novel-protein-c3orf43-accelerates-hepatocyte-proliferation
#1
Chunyan Zhang, Cuifang Chang, Deming Li, Fuchun Zhang, Cunshuan Xu
BACKGROUND: Our previous study found that single-pass membrane protein with coiled-coil domains 1 (C3orf43; XM_006248472.3) was significantly upregulated in the proliferative phase during liver regeneration. This indicates that C3orf43 plays a vital role in liver cell proliferation. However, its physiological functions remains unclear. METHODS: The expressions of C3orf43 in BRL-3A cells transfected with C3orf43-siRNA (C3-siRNA) or overexpressing the vector plasmid pCDH-C3orf43 (pCDH-C3) were measured via RT-qPCR and western blot...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28878809/are-online-prediction-tools-a-valid-alternative-to-genomic-profiling-in-the-context-of-systemic-treatment-of-er-positive-breast-cancer
#2
REVIEW
Umar Wazir, Kinan Mokbel, Amtul Carmichael, Kefah Mokbel
BACKGROUND: Clinicians use clinical and pathological parameters, such as tumour size, grade and nodal status, to make decisions on adjuvant treatments for breast cancer. However, therapeutic decisions based on these features tend to vary due to their subjectivity. Computational and mathematical algorithms were developed using clinical outcome data from breast cancer registries, such as Adjuvant! Online and NHS PREDICT. More recently, assessments of molecular profiles have been applied in the development of better prognostic tools...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28878808/luteolin-quercetin-genistein-and-quercetagetin-inhibit-the-effects-of-lipopolysaccharide-obtained-from-porphyromonas-gingivalis-in-h9c2-cardiomyoblasts
#3
Gloria Gutiérrez-Venegas, Alfredo Torras-Ceballos, Juan Arturo Gómez-Mora, Berenice Fernández-Rojas
BACKGROUND: One of the microorganisms from dental plaque associated with severe inflammatory responses in infectious endocarditis is Porphyromonas gingivalis. It is a Gram-negative bacteria harvested from chronic periodontitis patients. Lipopolysaccharide (LPS) obtained from P. gingivalis promotes the expressions of interleukin-1 (IL-1), IL-6 and tumor necrosis factor alpha (TNF-α). Flavonoids are thought to participate in processes that control inflammation, such as the expression of cyclooxygenase-2 (COX-2)...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28861108/gene-functioning-and-storage-within-a-folded-genome
#4
REVIEW
Sergey V Razin, Sergey V Ulianov
In mammals, genomic DNA that is roughly 2 m long is folded to fit the size of the cell nucleus that has a diameter of about 10 μm. The folding of genomic DNA is mediated via assembly of DNA-protein complex, chromatin. In addition to the reduction of genomic DNA linear dimensions, the assembly of chromatin allows to discriminate and to mark active (transcribed) and repressed (non-transcribed) genes. Consequently, epigenetic regulation of gene expression occurs at the level of DNA packaging in chromatin. Taking into account the increasing attention of scientific community toward epigenetic systems of gene regulation, it is very important to understand how DNA folding in chromatin is related to gene activity...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28861107/piwi-like-protein-hiwi2-is-aberrantly-expressed-in-retinoblastoma-cells-and-affects-cell-cycle-potentially-through-otx2
#5
Suganya Sivagurunathan, Jayamuruga Pandian Arunachalam, Subbulakshmi Chidambaram
Retinoblastoma (RB), a childhood cancer, is caused by biallelic mutation of the RB1 gene, but its development is not clearly understood. Furthermore, the presence of a cancer stem cell subpopulation in RB might impact its treatment. PIWI protein, known for its role in stem cell self-renewal, is aberrantly expressed in cancers. We examined the role of the PIWI-like protein HIWI2 in RB and its effect on the stem cell markers in cells of the RB line, Y79. The expression of HIWI2 is significantly increased in Y79 compared with its level in HeLa and ARPE19 cells...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28828010/adam23-promotes-neuronal-differentiation-of-human-neural-progenitor-cells
#6
Annett Markus-Koch, Oliver Schmitt, Susanne Seemann, Jan Lukas, Dirk Koczan, Mathias Ernst, Georg Fuellen, Andreas Wree, Arndt Rolfs, Jiankai Luo
BACKGROUND: ADAM23 is widely expressed in the embryonic central nervous system and plays an important role in tissue formation. RESULTS: In this study, we showed that ADAM23 contributes to cell survival and is involved in neuronal differentiation during the differentiation of human neural progenitor cells (hNPCs). Upregulation of ADAM23 in hNPCs was found to increase the number of neurons and the length of neurite, while its downregulation decreases them and triggers cell apoptosis...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28808448/relevance-of-the-nr4a-sub-family-of-nuclear-orphan-receptors-in-trophoblastic-bewo-cell-differentiation
#7
Sudha Saryu Malhotra, Satish Kumar Gupta
Nur-77, a member of the NR4A sub-family of nuclear orphan receptors, is downregulated in the placentae of pre-eclamptic women. Here, we investigate the relevance of Nor-1, Nurr-1 and Nur-77 in trophoblastic cell differentiation. Their transcript levels were found to be significantly upregulated in BeWo cells treated with forskolin. The maximum increase was observed after 2 h, with a second peak in the expression levels after 48 h. The expression of NR4A sub-family members was also found to be upregulated in BeWo cells after treatment with hCG and GnRH...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28794794/sfrp2-enhances-the-osteogenic-differentiation-of-apical-papilla-stem-cells-by-antagonizing-the-canonical-wnt-pathway
#8
Luyuan Jin, Yu Cao, Guoxia Yu, Jinsong Wang, Xiao Lin, Lihua Ge, Juan Du, Liping Wang, Shu Diao, Xiaomeng Lian, Songlin Wang, Rui Dong, Zhaochen Shan
BACKGROUND: Exploring the molecular mechanisms underlying directed differentiation is helpful in the development of clinical applications of mesenchymal stem cells (MSCs). Our previous study on dental tissue-derived MSCs demonstrated that secreted frizzled-related protein 2 (SFRP2), a Wnt inhibitor, could enhance osteogenic differentiation in stem cells from the apical papilla (SCAPs). However, how SFRP2 promotes osteogenic differentiation of dental tissue-derived MSCs remains unclear...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28747926/agonist-induced-activation-of-human-ffa1-receptor-signals-to-extracellular-signal-regulated-kinase-1-and-2-through-gq-and-gi-coupled-signaling-cascades
#9
Jing Qian, Yuyang Gu, Chun Wu, Feng Yu, Yuqi Chen, Jingmei Zhu, Xingyi Yao, Chen Bei, Qingqing Zhu
BACKGROUND: FFA1 is abundantly expressed in the liver, skeletal muscle, monocytes and nervous system, but is particularly abundant in pancreatic β cells. It is widely believed that FFA1 exerts its regulatory roles in a variety of physiological and pathological functions. In response to oleic acid, FFA1 has been shown to induce the activation of extracellular signal-regulated kinase 1 and 2 (ERK1/2) through a mechanism involving EGFR transactivation in a breast cancer cell line. However, the underlying molecular mechanism for ERK1/2 activation mediated by n-6 free fatty acid (LA) in HEK293 cells remains to be further elucidated...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28725241/mir-378-suppresses-the-proliferation-migration-and-invasion-of-colon-cancer-cells-by-inhibiting-sdad1
#10
Mingxi Zeng, Linlin Zhu, Liangping Li, Changming Kang
BACKGROUND: MicroRNAs (miRNAs) play important roles in the growth and metastasis of colon cancer. It is known that one set of miRNAs are dysregulated in colon cancer cells, but the mechanism of their role in cancer development is still largely unknown. Our study focuses on the role of miR-378 in colon cancer cells. METHODS: Human colon cancer tissues and adjacent non-tumor tissues were collected from patients diagnosed in pathological examinations. In addition, human colon cancer cell lines LoVo, CaCo2, SW1116, SW480 and HCT-116, and a normal colonic mucosa cell line NCM460 were included...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28717367/cells-of-human-breast-milk
#11
REVIEW
Malgorzata Witkowska-Zimny, Ewa Kaminska-El-Hassan
Human milk is a complex fluid that has developed to satisfy the nutritional requirements of infants. In addition to proteins, lipids, carbohydrates and other biologically active components, breast milk contains a diverse microbiome that is presumed to colonize the infant gastrointestinal tract and a heterogeneous population of cells with unclear physiological roles and health implications. Noteworthy cellular components of breast milk include progenitor/stem cells. This review summarizes the current state of knowledge of breast milk cells, including leukocytes, epithelial cells, stem cells and potentially probiotic bacteria...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28652859/lncrna-h19-promotes-the-differentiation-of-bovine-skeletal-muscle-satellite-cells-by-suppressing-sirt1-foxo1
#12
Xiaochun Xu, Shengyue Ji, Weili Li, Bao Yi, Hengxin Li, Hongfu Zhang, Wenping Ma
BACKGROUND: H19 is a well-characterized Long noncoding RNA (lncRNA) that has been proven to promote myoblast differentiation in humans and mice. However, its mechanism of action is still not fully interpreted. METHODS: Using RT-qPCR, we examined H19 RNA levels in various tissues from 1-week, 1-month, 6-month and 36-month old male cattle (i.e., newborn, infant, young and adult). The protein and mRNA levels of MyoG, MyHC, Sirt1 and FoxO1 in the satellite and C2C12 cells with an H19 silencing or overexpression vector were respectively detected using western blot and real-time qPCR...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28536640/survival-rates-of-homozygotic-tp53-knockout-rats-as-a-tool-for-preclinical-assessment-of-cancer-prevention-and-treatment
#13
Damian Strzemecki, Magdalena Guzowska, Paweł Grieb
BACKGROUND: The gene that encodes tumor protein p53, Tp53, is mutated or silenced in most human cancers and is recognized as one of the most important cancer drivers. Homozygotic Tp53 knockout mice, which develop lethal cancers early in their lives, are already used in cancer prevention studies, and now Tp53 knockout rats have also been generated. This study assessed feasibility of using homozygous Tp53 knockout rats to evaluate the possible outcome of cancer chemoprevention. METHODS: A small colony of Tp53 knockout rats with a Wistar strain genetic background was initiated and maintained in the animal house at our institution...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28536639/survivin-a-molecular-target-for-therapeutic-interventions-in-squamous-cell-carcinoma
#14
REVIEW
Zakir Khan, Abdul Arif Khan, Hariom Yadav, Godavarthi B K S Prasad, Prakash Singh Bisen
Squamous cell carcinoma (SCC) is the most common cancer worldwide. The treatment of locally advanced disease generally requires various combinations of radiotherapy, surgery, and systemic therapy. Despite aggressive multimodal treatment, most of the patients relapse. Identification of molecules that sustain cancer cell growth and survival has made molecular targeting a feasible therapeutic strategy. Survivin is a member of the Inhibitor of Apoptosis Protein (IAP) family, which is overexpressed in most of the malignancies including SCC and totally absent in most of the normal tissues...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28536638/abcc6-knockdown-in-hepg2-cells-induces-a-senescent-like-cell-phenotype
#15
Rocchina Miglionico, Angela Ostuni, Maria Francesca Armentano, Luigi Milella, Elvira Crescenzi, Monica Carmosino, Faustino Bisaccia
BACKGROUND: Pseudoxanthoma elasticum (PXE) is characterized by progressive ectopic mineralization of elastic fibers in dermal, ocular and vascular tissues. No effective treatment exists. It is caused by inactivating mutations in the gene encoding for the ATP-binding cassette, sub-family C member 6 transporter (ABCC6), which is mainly expressed in the liver. The ABCC6 substrate (s) and the PXE pathomechanism remain unknown. Recent studies have shown that overexpression of ABCC6 in HEK293 cells results in efflux of ATP, which is rapidly converted into nucleoside monophosphates and pyrophosphate (PPi)...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28536637/ppar%C3%AE-agonist-through-the-terminal-differentiation-phase-is-essential-for-adipogenic-differentiation-of-fetal-ovine-preadipocytes
#16
Yong Pu, Almudena Veiga-Lopez
BACKGROUND: Although the 3T3-L1 preadipocyte cell line represents an informative model for in vitro adipogenesis research, primary cultured cells are often needed to understand particular human or animal metabolic phenotypes. As demonstrated by in vitro cultured preadipocytes from large mammalian species, primary cultured cells require specific adipogenic differentiation conditions different to that of the 3T3-L1 cell line. These conditions are also species-specific and require optimization steps...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28536636/a-simple-and-effective-protocol-for-fast-isolation-of-human-tenon-s-fibroblasts-from-a-single-trabeculectomy-biopsy-a-comparison-of-cell-behaviour-in-different-culture-media
#17
Agata Przekora, Tomasz Zarnowski, Grazyna Ginalska
BACKGROUND: Human Tenon's fibroblasts (HTFs) play a crucial role in wound healing. They cause postoperative scarring of the filtering bleb and are thus responsible for trabeculectomy failure. This study aimed to find an effective and fast protocol for HTF isolation from trabeculectomy biopsies. The protocol was compared with the commonly recommended HTF isolation procedure, which uses Dulbecco's modified Eagle's medium (DMEM). We used Eagle's minimum essential medium (EMEM) enriched with fibroblast growth factor (FGF), which selectively promoted the proliferation of HTF cells...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28536635/the-regulatory-mechanisms-of-ng2-cspg4-expression
#18
REVIEW
Emmanuel Ampofo, Beate M Schmitt, Michael D Menger, Matthias W Laschke
Neuron-glial antigen 2 (NG2), also known as chondroitin sulphate proteoglycan 4 (CSPG4), is a surface type I transmembrane core proteoglycan that is crucially involved in cell survival, migration and angiogenesis. NG2 is frequently used as a marker for the identification and characterization of certain cell types, but little is known about the mechanisms regulating its expression. In this review, we provide evidence that the regulation of NG2 expression underlies inflammation and hypoxia and is mediated by methyltransferases, transcription factors, including Sp1, paired box (Pax) 3 and Egr-1, and the microRNA miR129-2...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28536634/microrna-210-induces-endothelial-cell-apoptosis-by-directly-targeting-pdk1-in-the-setting-of-atherosclerosis
#19
Ying Li, Chunyan Yang, Lili Zhang, Ping Yang
BACKGROUND: Atherosclerosis is a chronically inflammatory disease and one of the leading causes of deaths worldwide. Endothelial cell apoptosis plays a crucial role in its development. Several microRNAs (miRNAs) are reportedly involved in atherosclerotic plaque formation, including miRNA-210 (miR-210). However, the underlying mechanism of its role in endothelial cell apoptosis during atherosclerosis is still largely unknown. METHODS: A mouse model with atherosclerosis induced by a high-fat diet (HFD) was built in ApoE (-/-) mice...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28536633/mitf-and-pu-1-inhibit-adipogenesis-of-ovine-primary-preadipocytes-by-restraining-c-ebp%C3%AE
#20
ChongMei Ruan, Xiu Li, JunJie Hu, Yong Zhang, XingXu Zhao
BACKGROUND: PU box-binding protein (PU.1) is a master gene of hematopoietic lineage and an important specific transcription factor in osteoclast lineage. There is proof of its expression in adipose tissue, and it is known to significantly and negatively affect adipogenesis. However, it is unclear whether there are any other molecules involved in this process. METHODS: We wished to explore the effect of PU.1's co-activator microphthalmia-associated transcription factor (MITF) on the adipogenic differentiation of ovine primary preadipocytes...
2017: Cellular & Molecular Biology Letters
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