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Cellular & Molecular Biology Letters

Yafeng Liang, Nengli Yang, Guoquan Pan, Bingxin Jin, Shufen Wang, Wei Ji
Background: Pulmonary inflammation and endothelial barrier permeability increase in acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) induced by pro-inflammatory cytokines and matrix metalloproteinases (MMPs). However, the relationship between pro-inflammatory cytokines and MMPs in ALI/ARDS remains poorly understood. Methods: A lipopolysaccharide (LPS)-induced ALI rat model was established through intratracheal instillation. The wet/dry ratios of lung tissues were measured, and bronchoalveolar lavage fluid (BALF) was collected to test protein concentrations, total cell/macrophage numbers, and pro-inflammatory cytokine levels...
2018: Cellular & Molecular Biology Letters
Shadan Hajalirezay Yazdi, Mahdi Paryan, Samira Mohammadi-Yeganeh
Background: Breast cancer is the most prevalent cancer among women, and AXL and MET are the key genes in the PI3K/AKT/mTOR pathway as critical elements in proliferation and invasion of cancer cells. MicroRNAs (miRNAs) are small non-coding RNAs regulating the expression of genes. Methods: Bioinformatic approaches were used to find a miRNA that simultaneously targets both AXL and MET 3'-UTRs. The expression of target miRNA was evaluated in triple-negative (MDA-MB-231) and HER2-overexpressing (SK-BR-3) breast cancer cell lines as well as normal breast cells, MCF-10A, using quantitative real-time PCR...
2018: Cellular & Molecular Biology Letters
Yonghua Xu, Xiangmin Wang, Surong Jiang, Chen Men, Di Xu, Yan Guo, Jun Wu
Background: Microcystins are waterborne environmental toxins that induce oxidative stress and cause injuries in the heart. On the other hand, many physiological processes, including antioxidant defense, are under precise control by the mammalian circadian clock. Results: In the present study, we evaluated the effect of microcystin-LR (MC-LR) on the rhythmic expression patterns of circadian and antioxidant genes in rat cardiomyocytes using the serum shock technique...
2018: Cellular & Molecular Biology Letters
Toru Miyanaga, Yoshimichi Ueda, Aiko Miyanaga, Mikio Yagishita, Naoko Hama
To date, studies on mesenchymal tissue stem cells (MSCs) in the perichondrium have focused on in vitro analysis, and the dynamics of cartilage regeneration from the perichondrium in vivo remain largely unknown. We have attempted to apply cell and tissue engineering methodology for ear reconstruction using cultured chondrocytes. We hypothesized that by inducing angiogenesis with basic fibroblast growth factor (bFGF), MSCs or cartilage precursor cells would proliferate and differentiate into cartilage in vivo and that the regenerated cartilage would maintain its morphology over an extended period...
2018: Cellular & Molecular Biology Letters
Grey W Fortenbery, Brinda Sarathy, Kristen R Carraway, Kyle D Mansfield
Background: Tissue ischemia can arise in response to numerous physiologic and pathologic conditions. The cellular response to decreased perfusion, most notably a decrease in glucose and oxygen, is important for cellular survival. In response to oxygen deprivation or hypoxia, one of the key response elements is hypoxia inducible factor (HIF) and a key protein induced by hypoxia is vascular endothelial growth factor (VEGF). Under hypoxia, we and others have reported an increase in the half-life of VEGF and other hypoxia related mRNAs including MYC and CYR61; however, the mediator of this response has yet to be identified...
2018: Cellular & Molecular Biology Letters
Zhong-Shi Xu, Feng Dai, Ji Chen, Meng Lv, Ji-Wu Cheng, Xiao-Ming Zhang, Bo-Wen Lin
Background: Evidence has shown that endogenous H2 S plays an important role in the physiological and pathophysiological processes of many organs. The study aimed to explore whether exogenous H2 S has a potential therapeutic effect on a rat ovariectomy-induced model of osteoporosis. Methods: The OVX osteoporosis model was established in female Sprague-Dawley rats by full bilateral ovariectomy. The rats were randomly divided into four groups, with the two experimental groups receiving an intraperitoneal injection of GYY4137 or sodium alendronate...
2018: Cellular & Molecular Biology Letters
Maoyun Fei, Jianming Guan, Tao Xue, Lianjin Qin, Chengwu Tang, Ge Cui, Yao Wang, Hui Gong, Wenming Feng
Background: Hepatocellular carcinoma (HCC) is the fifth most common cancer and the third most common cause of cancer-related death worldwide. The 5-year survival rate remains low despite considerable research into treatments of HCC, including surgery, radiotherapy and chemotherapy. Many mechanisms within HCC still require investigation, including the influence of hypoxia, which has a crucial role in many cancers and is associated with metastasis. Hypoxia inducible factor-1α (HIF-1α) is known to regulate the expression of many chemokines, including interleukin-8 (IL-8), which is associated with tumor metastasis...
2018: Cellular & Molecular Biology Letters
Rafal Bartoszewski, Aleksander F Sikorski
Recent developments in high-throughput genotyping technologies have revealed the existence of several new classes of RNA that do not encode proteins but serve other cellular roles. To date, these non-coding RNAs (ncRNAs) have been shown to modulate both gene expression and genome remodeling, thus contributing to the control of both normal and disease-related cellular processes. The attraction of this research topic can be seen in the increasing number of submissions on ncRNAs to molecular biology journals, including Cellular Molecular Biology Letters (CMBL)...
2018: Cellular & Molecular Biology Letters
Yanxia Wang, Hui Xu, Baoan Zhu, Zhenling Qiu, Zaijun Lin
Background: Tumor microenvironment, in particular the stroma, plays an important role in breast cancer cell invasion and metastasis. Investigation of the molecular characteristics of breast cancer stroma may reveal targets for future study. Methods: The transcriptome profiles of breast cancer stroma and normal breast stroma were compared to identify differentially expressed genes (DEGs). The method was analysis of GSE26910 and GSE10797 datasets. Common DEGs were identified and then analyses of enriched pathways and hub genes were performed...
2018: Cellular & Molecular Biology Letters
Lifan Zhuang, Jianhua Tian, Xinzhi Zhang, Hong Wang, Chenghui Huang
Background: Lnc-DC is a specific group of long non-coding (Lnc) RNAs in dendritic cells (DCs). Its function has been previously studied, and includes roles in dendritic cell differentiation and the progression of some diseases. In this study, we observed the critical role of Lnc-DC in regulating the differentiation, growth, and apoptosis of dendritic cells. Methods: We first isolated peripheral blood mononuclear cells to culture and induce into DCs, which were then co-cultured with hepatitis B virus (HBV)-secreting HepG2...
2018: Cellular & Molecular Biology Letters
Mohammad Reza Abbaszadegan, Anali Riahi, Mohammad Mahdi Forghanifard, Meysam Moghbeli
Background: Esophageal squamous cell carcinoma (ESCC) is the most common histological type of esophageal cancer, with a poor prognosis. Deregulation of WNT and NOTCH signaling pathways is important in ESCC progression, which can be due to either malfunction of their components or crosstalk with other pathways. Therefore, identification of new crosstalk between such pathways may be effective to introduce new strategies for targeted therapy of cancer. A correlation study was performed to assess the probable interaction between growth factor receptors and WNT/NOTCH pathways via the epidermal growth factor receptor (EGFR) and Musashi1 (MSI1), respectively...
2018: Cellular & Molecular Biology Letters
Zhi-Qiang Tian, Hong Jiang, Zhi-Bing Lu
Background: MicroRNAs play important roles in regulation of the cardiovascular system. The purpose of this study was to investigate microRNA-320 (miR-320) expression in myocardial ischemia-reperfusion (I/R) injury and the roles of miR-320 in cardiomyocyte apoptosis by targeting AKIP1 (A kinase interacting protein 1). Methods: The level of miR-320 was detected using quantitative real-time polymerase chain reaction (qRT-PCR), and cardiomyocyte apoptosis was detected via terminal dUTP nick end-labeling assay...
2018: Cellular & Molecular Biology Letters
Xin Ni, Xin-Zhi Li, Zhi-Ru Fan, Ai Wang, Hai-Chao Zhang, Liang Zhang, Li Li, Jun-Qiang Si, Ke-Tao Ma
Background: Imbalances in circulating T lymphocytes play critical roles in the pathogenesis of hypertension-mediated inflammation. Connexins (Cxs) in immune cells are involved in the maintenance of homeostasis of T lymphocytes. However, the association between Cxs in peripheral blood T lymphocytes and hypertension-mediated inflammation remains unknown. This study was designed to investigate the role of Cxs in T lymphocytes in hypertension-mediated inflammation in spontaneously hypertensive rats (SHRs)...
2018: Cellular & Molecular Biology Letters
Fengrui Wu, Qingqing Wu, Dengkun Li, Yuan Zhang, Rong Wang, Yong Liu, Wenyong Li
Background: The transcription factor Oct4 plays a pivotal role in the pre-implantation development of the mouse embryo. DNA methyltransferase 1 (Dnmt1) maintains the changes in DNA methylation during mammalian early embryonic development. Little is known of the role of Oct4 in DNA methylation in mice. In this study, Kunming white mice were used as an animal model to reveal any correlation between DNA methylation and Oct4 during mammalian embryonic development. Results: The expressions of Dnmt1 and Oct4 were initially studied using real-time PCR...
2018: Cellular & Molecular Biology Letters
Guangzhe Yu, Qian Liu, Xuening Dong, Kaihong Tang, Bohui Li, Chunmei Liu, Wenzheng Zhang, Yiduo Wang, Yingyu Jin
Background: Inflammation is an important pathogenic component of endotoxemia-induced acute kidney injury (AKI), finally resulting in renal failure. Diacerein is an interleukin-1β (IL-1β) inhibitor used for osteoarthritis treatment by exerting anti-inflammatory effects. This study aims to investigate the effects of diacerein on endotoxemia-induced AKI. Methods: Male C57BL/6 mice were intraperitoneally injected with lipopolysaccharide (LPS, 10 mg/kg) for 24 h prior to diacerein treatment (15 mg/kg/day) for another 48 h...
2018: Cellular & Molecular Biology Letters
Jian-Peng Hu, Rong Zhang, Min Tang, Yu-Lian Li, Lin-Ting Xun, Zhi-Zhou Shi, Ying An, Ting Li, Zheng-Ji Song
Background: We investigated the activity of loureirin B against liver fibrosis and the underlying molecular mechanisms. Methods: Hepatic stellate cells (HSCs) from Sprague-Dawley rats were treated with different concentrations of loureirin B. We used the MTT assay to determine HSC proliferation, flow cytometry to analyze apoptosis, and western blot to determine the expressions of Bax, Bcl-2, Wnt1 and β-catenin. Real-time PCR was used to determine the expressions of Wnt1 and miR-148-3p...
2018: Cellular & Molecular Biology Letters
Wei Wu, Li Nie, Li Zhang, Yan Li
Background: Oncogenic Notch1 is known to activate the NF-κB pathway in T cell acute lymphoblastic leukemia (T-ALL) and to up-regulate the transcription of Asb2α, a specificity factor for an E3 ubiquitin ligase complex that plays an important role in hematopoietic differentiation. Therefore, we hypothesize that Notch1 might regulate the NF-κB pathway through Asb2α. Methods: The study involved down-regulation of Notch1 in T-ALL cell lines (CCRF-CEM cells and MOLT-4 cells) through treatment with gamma-secretase inhibitor (GSI) as well as the modulation of Asb2 in CCRF-CEM cells and MOLT-4 cells through transduction with lentivirus carrying Asb2 or Asb2 -shRNA...
2018: Cellular & Molecular Biology Letters
Liqun Chen, Lingjuan Wu, Linyan Zhu, Yiyi Zhao
The nuclear receptor RXRα (retinoid X receptor-α) is a transcription factor that regulates the expression of multiple genes. Its non-genomic function is largely related to its structure, polymeric forms and modification. Previous research revealed that some non-genomic activity of RXRα occurs via formation of heterodimers with Nur77. RXRα-Nur77 heterodimers translocate from the nucleus to the mitochondria in response to certain apoptotic stimuli and this activity correlates with cell apoptosis. More recent studies revealed a significant role for truncated RXRα (tRXRα), which interacts with the p85α subunit of the PI3K/AKT signaling pathway, leading to enhanced activation of AKT and promoting cell growth in vitro and in animals...
2018: Cellular & Molecular Biology Letters
Ying Huang, Wuping Yang, Huihong Zeng, Chuan Hu, Yaqiong Zhang, Nanhua Ding, Guangqin Fan, Lijian Shao, Bohai Kuang
Upregulation of histone acetylation plays a critical role in the dysregulation of transcription. It alters the structure of chromatin, which leads to the onset of cancer. Histone deacetylase inhibitors may therefore be a promising way to limit cancer progression. In this study, we examined the effects of droxinostat on the growth of HT-29 colon cancer cells. Our results show that droxinostat effectively inhibited cell growth and colony-forming ability by inducing cellular apoptosis and ROS production in HT-29 cells...
2018: Cellular & Molecular Biology Letters
Shaoxian Liu, Junyi Yue, Wei Du, Jian Han, Weidong Zhang
Background: Osteosarcoma (OS) is a common malignant tumor that predominantly occurs in adolescents. Its most common metastasis is to the lungs. As shown in our earlier study, lysosome-associated membrane glycoprotein 3 (LAMP3) is highly upregulated in metastatic OS. However, its role in the regulation of OS cell viability and apoptosis remains unknown. Methods: We knocked down and overexpressed LAMP3 in OS cells and assessed the cell viability and apoptosis. Then, we investigated the expression of apoptosis-associated genes to identify the downstream gene(s) of LAMP3 ...
2018: Cellular & Molecular Biology Letters
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