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Preparative Biochemistry & Biotechnology

Johann Bauer, Hari H P Cohly, Jayashree Sahana, Daniela Grimm
Human cells are heterogeneous in regard to their biochemical features and functions. Detailed knowledge about each single cell type is important to understand the whole organism. In order to get a deeper insight in the concert of life, it has to be considered that cell populations such as thyroid cells, epithelial breast cells, endothelial cells, or chondrocytes are heterogeneous in regard to function, RNA expression patterns and protein content. This is true for normal cells and even more relevant for cancer cells...
November 5, 2018: Preparative Biochemistry & Biotechnology
Yasemin Budama-Kilinc, Rabia Cakir-Koc, Burak Ozdemir, Zeynep Kaya, Selim Badur
Antibodies play an important role in combating and controlling viral diseases such as influenza. Immunoglobulin Y (IgY) antibodies have several advantages such as a less invasive manufacturing process, ease of isolation, higher affinity compared with IgG antibodies, and cost-effectiveness. To date, although specific IgY production has been performed for different strains of influenza A, to the best of our knowledge, an IgY against the M2e peptide has not been produced. In the current study, IgY antibodies are produced, purified, and characterized using the M2e peptide sequence for the first time with the intent to apply them for the diagnosis of influenza A virus...
November 2, 2018: Preparative Biochemistry & Biotechnology
Monike Fabiane Alves Ribeiro Lacerda, Flávio Marques Lopes, Adilson Sartoratto, Alexandre Nunes Ponezi, Douglas Vieira Thomaz, Fernando Schimidt, Mariângela Fontes Santiago
In this work were studied the pH, thermal, and storage stability of free and immobilized laccases. Enzymes were produced by Pleurotus ostreatus on potato dextrose (PD) broth and potato dextrose modified (PDM) broth, and immobilized using Luffa cylindrica fibers as support. Both free and immobilized enzymes were assessed on their respective enzymatic activities and for 17α-ethinylestradiol (EE2) degradation. The optimum pH conditions concerning laccase activity ranged from 3.6 to 4.6, while temperature ranged between 30 °C and 50 °C for both free and immobilized enzyme...
November 2, 2018: Preparative Biochemistry & Biotechnology
Guanjun Nan, Yuqiong Gao, Liying Guo, Xianxin Meng, Guangde Yang
The extraction of daidzein and genistein from soybean has been studied and the kinetic modeling was established using four modeling equations. The goodness of fit was evaluated by statistical errors including the standard error of means (SEM), the adjusted correlation coefficient (R2 ), and chi-square (χ2 ). The best model was considered to be the So and Macdonald model and it could give the most adequate description of solid-liquid extraction of daidzein and genistein from soybean sample. The effect of process parameters on extraction yields of daidzein and genistein also has been investigated...
November 2, 2018: Preparative Biochemistry & Biotechnology
Yongmei Liu, Zhonghua Wang, Mingyuan Liu, Lin Zhou, Yunying Sha
This work aims to enhance the bioproduction of xanthan gum by screening a hyper-yield producer from the wild-type Xanthomonas campestris during a long-term continuous subculture. We reported a cell-wall deficient mutant, which performed a shift of cell morphology from rod-shaped to round-shaped. Both the yield of xanthan gum and the conversion rate of feedstock were assessed using sucrose as a carbon source with the supplement of yeast extract powder, l-glutamic acid, and other raw materials. After 96 h aerobic fermentation, the yield of xanthan gum of the mutant reached up to 32 g/L, which was 3...
November 2, 2018: Preparative Biochemistry & Biotechnology
Dhiraj Kumar Choudhary, Abha Mishra
Imbalance of free radicals over antioxidants in human body may result in oxidative damage to biomolecules (lipids, proteins, DNA) that causes severe chronic diseases. The aim of this proposed research was to examine the hypoglycemic and oxidative stress potential of fava bean (Vicia faba L.) seed extract. Acetone extract showed a significant effect on glucose uptake rate (77.28 ± 2.42%) in yeast cells at 25 mM glucose concentration. Minimum glucose uptake rate was found to be 52.36 ± 2.06% % by chloroform seed extract...
November 2, 2018: Preparative Biochemistry & Biotechnology
Subbalaxmi Selvaraj, Ramachandra Murty Vytla
Production of tannase was performed in packed bed reactor filled with an inert support polyurethane foam (PUF) using Bacillus gottheilii M2S2. The influence of process parameters such as fermentation time (24-72 h), tannic acid concentration (0.5-2.5% w/v), inoculum size (7-12% v/v), and aeration rate (0-0.2 L/min) on tannase production with PUF were analyzed using one variable at a time (OVAT) approach. The outcome of OVAT was optimized by central composite design. Based on the statistical investigation, the proposed mathematical model recommends 1% (w/v) of tannic acid, 10% (v/v) of inoculum size and 0...
October 10, 2018: Preparative Biochemistry & Biotechnology
Gayathri Gopu, Srinikethan Govindan
Bacterial cellulose (BC) is one of the prominent biopolymers that has been acquiring attention currently due to its distinctive properties and applications in various fields. The current work presents the isolation of Komagataeibacter saccharivorans strain BC1 isolated from rotten green grapes, followed by biochemical and genotypic characterization, which confirmed that the strain is capable of synthesizing cellulose. Further, production media was designed and certain variables such as carbon, nitrogen sources, pH, and temperature were optimized in order to obtain the maximum concentration of cellulose production...
October 10, 2018: Preparative Biochemistry & Biotechnology
Rashmi Bommenahalli Shashidhara, Regupathi Iyyaswami
Pisum sativum lectin (Psl) is a metalloprotein which is in the center of research interest because of its HIV-1 reverse transcriptase inhibitory activity and mitogenic activity. The application of this lectin in various fields demands the economically feasible and scalable purification strategy other than affinity chromatography. The suitability of aqueous two phase system (ATPS) composed of poly ethylene glycol (PEG) with different salts (sodium citrate, potassium citrate, and ammonium citrate) was evaluated for better partitioning of Psl...
October 10, 2018: Preparative Biochemistry & Biotechnology
Subramanian Prathiba, Gurunathan Jayaraman
The objective was to evaluate the anticancer and antioxidant activities of the methanolic extracts of halophilic bacteria, isolated from soil samples of Marakkanam saltern and Pichavaram mangrove forest, India. Radical Scavenging activity, reducing power, and metal ion chelation ability was used to evaluate the antioxidant potential of the metabolic extracts, whereas cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The methanolic extract of Bacillus VITPS7 exhibited significant antioxidant property...
October 10, 2018: Preparative Biochemistry & Biotechnology
Tobias Pereira de Morais, Paula Mirella Gomes Barbosa, Nayara Fernanda Lisboa Garcia, Nathália Gonsales da Rosa-Garzon, Gustavo Graciano Fonseca, Marcelo Fossa da Paz, Hamilton Cabral, Rodrigo Simões Ribeiro Leite
The objective of the present study was to optimize parameters for the cultivation of Lichtheimia corymbifera (mesophilic) and Byssochlamys spectabilis (thermophilic) for the production of β-glucosidases and to compare the catalytic and thermodynamic properties of the partially purified enzymes. The maximum amount of β-glucosidase produced by L. corymbifera was 39 U/g dry substrate (or 3.9 U/mL), and that by B. spectabilis was 77 U/g (or 7.7 U/mL). The optimum pH and temperature were 4.5 and 55 °C and 4...
October 10, 2018: Preparative Biochemistry & Biotechnology
Rajkumar Paul, Selvarajan Karthik, Ponnusamy Vimalraj, Sankaranarayanan Meenakshisundaram, Perumal Kaliraj
Lymphatic filariasis is a "disease of poor people" due to a large section of affected people with economic backwardness. Therefore, successful elimination of this disease requires a cost-effective prophylactic agent such as vaccine along with conventional drugs. The Abundant Larval Transcript-2 (BmALT-2) protein of Brugia malayi has been recognized as the most potential vaccine candidate. Tuftsin, a tetra-peptide immunopotentiator has already shown the enhanced immunogenicity of various vaccine antigens in earlier studies...
October 10, 2018: Preparative Biochemistry & Biotechnology
Chirom Aarti, Ameer Khusro, Paul Agastian
In this context, carboxymethyl cellulase (CMCase) production from Glutamicibacter arilaitensis strain ALA4 was initially optimized by one factor at a time (OFAT) method using goat dung as proficient feedstock. Two-level full factorial design (25 factorial matrix) using first-order polynomial model revealed the significant (p < 0.05) influence of pH, moisture, and peptone on CMCase activity. Central composite design at N = 20 was further taken into account using a second-order polynomial equation, and thereby liberated maximum CMCase activity of 4925...
October 10, 2018: Preparative Biochemistry & Biotechnology
Haiyan Liu, Rongzhen Zhang, Lihong Li, Lixian Zhou, Yan Xu
The hydrolysates of soy protein and milk protein are nutritional and functional food ingredients. Aspergillus pseudoglaucus aspergillopepsin I (App) is an acidic protease, including signal peptide, propeptide, and catalytic domain. Here, we cloned the catalytic domain App with or without propeptide in Escherichia coli. The results showed that the App without propeptide was not expressed or did not exhibit activity and App with propeptide (proApp) was highly expressed with a specific activity of 903 U/mg. Moreover, the denaturation temperature of proApp was 4...
October 10, 2018: Preparative Biochemistry & Biotechnology
Dulce Jiménez-Barrera, Wilberth Chan-Cupul, Zhiliang Fan, Juan A Osuna-Castro
The optimization of ligninolytic enzyme (LE) activities in a novel fungal co-culture between Pycnoporus sanguineus and Beauveria brongniartii were studied using a Plackett-Burman experimental design (PBED) and a central composite design (CCD). In addition, H2 O2 role was analyzed. Laccase (EC. and MnP (EC activities of P. sanguineus increased 6.0- and 2.3-fold, respectively, in the co-culture with B. brongniartii. The H2 O2 content was higher in the co-culture (0.33-7.12-fold) than in the P...
October 10, 2018: Preparative Biochemistry & Biotechnology
Renpeng Du, Fangkun Zhao, Xiaoxiao Qiao, Qiaozhi Song, Guangbin Ye, Yu Wang, Binbin Wang, Ye Han, Zhijiang Zhou
Strain Bacillus amyloliquefaciens BH1 was evaluated for the generation of α-amylase. Culture conditions and medium components were optimized by a statistical approach for the optimal generation of α-amylase with response surface methodology (RSM) method. The Plackett-Burman (PB) design was executed to select the fermentation variables and Central composite design (CCD) for optimizing significant factors influencing production. The optimum levels for highest generation of α-amylase activity (198.26 ± 3...
October 10, 2018: Preparative Biochemistry & Biotechnology
Selvaraju Sivamani, Rajoo Baskar
Bioethanol production from agro-industrial residues is gaining attention because of the limited production of starch grains and sugarcane, and food-fuel conflict. The aim of the present study is to maximize the bioethanol production using cassava bagasse as a feedstock. Enzymatic liquefaction, by α-amylase, followed by simultaneous saccharification and fermentation (SSF), using glucoamylase and Zymomonas mobilis MTCC 2427, was investigated for bioethanol production from cassava bagasse. The factors influencing ethanol production process were identified and screened for significant factors using Plackett-Burman design...
October 10, 2018: Preparative Biochemistry & Biotechnology
Razieh Jafari Hajati, Vahide Payamnoor, Najmeh Ahmadian Chashmi, Kamal Ghasemi Bezdi
Betulin (B) and betulinic acid (BA) are two triterpenes with diverse pharmacological and physiological actions. Elicitation of Betula pendula Roth cell cultures by elicitors is an excellent strategy to increase B and BA levels. Six abiotic and biotic elicitors were studied to improve accumulation of B and BA in the cell culture of B. pendula. The B and BA production in treated cells was verified by HPLC. The results showed the maximum growth index (7) on day 3 in cells treated with 0.5 mg L-1 chlorocholine chloride (CCC)...
October 8, 2018: Preparative Biochemistry & Biotechnology
Elif Ozyilmaz, Kubra Etci, Mehmet Sezgin
The aim of this study was to prepare the encapsulation of Candida rugosa lipase (CRL) with magnetic sporopollenin. The sporopollenin was covalent immobilized onto magnetic nanoparticles (Fe3 O4 ), grafted amino (APTES), or epoxy groups (EPPTMS). CRL was sol-gel encapsulated in the presence of magnetic sporopollenin/Fe3 O4 nanoparticles. The influence of activation agents ([3-(2,3-epoxypropoxy) propyl] trimethoxysilane (EPPTMS), (3-Aminopropyl)triethoxysilane (APTES) and pH and thermal stabilities of the biocatalyst were assessed...
October 8, 2018: Preparative Biochemistry & Biotechnology
Dongxin Zhao, Zhongxian Huang, Jie Liu, Li Ma, Juan He
Zinc finger protein ZNF191(243-368), the zinc finger region of ZNF191, is potentially associated with cell proliferation in hepatocellular carninoma. A His-tag expression system was used to express and purify proteins with mutations in the zinc finger 3 of ZNF191(243-368) for analysis of protein properties, structure, and functions. The purification of the His-tag fusion proteins was simpler and faster than that of the ZNF191(243-368) inclusion bodies. The properties and structures of the His-tag fusion mutant proteins were investigated using spectrographic techniques and DNA hydrolysis experiment...
October 8, 2018: Preparative Biochemistry & Biotechnology
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