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Preparative Biochemistry & Biotechnology

Saima Nusrat, Rizwan Hasan Khan
The association of protein aggregates with plentiful human diseases has fascinated studies regarding the biophysical characterization of protein misfolding and ultimately their aggregate formation mechanism. Protein-ligand interaction, their mechanism, conformational changes by ligands and protein aggregate formation has been studied upon exploiting experimental techniques and computational methodologies. Such studies for the exploration of ligand induced conformational changes in protein, misfolding and aggregation, has confirmed drastic progresses in the study of aggregate formation pathways...
November 6, 2017: Preparative Biochemistry & Biotechnology
Manoj Kumar Narasimhan, Selvarajan Ethiraj, Tamilarasan Krishnamurthi, Mathur Rajesh
No abstract text is available yet for this article.
November 6, 2017: Preparative Biochemistry & Biotechnology
Guo-Li Gong, Yuan Wei, Zhong-Zhong Wang
In this study, a novel heterozygous antimicrobial peptide MLH was synthesized, expressed, purified and characterized. The peptide MLH was selected through bioinformatic analysis using musca domestica antimicrobial peptide (Cec-Med), human antimicrobial peptide LL-37 and helicobacter pylori antimicrobial peptide (Hp) as parent peptides. The target gene was synthesized by overlap extension PCR (SOE-PCR) and connected to the expression vector pET-32a (+), and the recombinant plasmid pET-32a-MLH was transformed to Escherichia coli for constructing pET-32a-MLH/BL21 (DE3)...
November 3, 2017: Preparative Biochemistry & Biotechnology
Zhen Rong, Ying-Yi Huo, Shu-Ling Jian, Yue-Hong Wu, Xue-Wei Xu
A novel esterase gene (e25) was identified from Altererythrobacter epoxidivorans CGMCC 1.7731(T) by genome sequence screening. The e25 gene is 948 nucleotides in length and encodes a 315 amino acid protein (E25) with a predicted molecular mass of 33,683 Da. A phylogenetic tree revealed that E25 belongs to the hormone-sensitive lipase (HSL) family of lipolytic enzymes. An activity assay of E25 showed that it exhibited the highest catalytic efficiency when using p-nitrophenyl caproate (C6) as a substrate. The optimum pH and temperature were determined to be approximately pH 9 and 45°C, and the Km and Vmax values were 0...
November 3, 2017: Preparative Biochemistry & Biotechnology
Thanongsak Chaiyaso, Atchara Manowattana
Bioconversion of biodiesel-derived crude glycerol into carotenoids and lipids was investigated by a microbial conversion of an oleaginous red yeast Sporidiobolus pararoseus KM281507. The methanol content in crude glycerol (0.5% w/v) did not show a significant effect on biomass production by strain KM281507. However, demethanolized crude glycerol significantly supported the production of biomass (8.64 ± 0.13 g/L), lipids (2.92 ± 0.03 g/L), β-carotene (15.76 ± 0.85 mg/L) and total carotenoids (33...
October 16, 2017: Preparative Biochemistry & Biotechnology
Priyanka V Shirodkar, Usha Devi Muraleedharan
Amylases are a group of enzymes with a wide variety of industrial applications. Enhancement of α-amylase production from the marine protists, thraustochytrids has been attempted for the first time by applying statistical based experimental designs using response surface methodology (RSM) and genetic algorithm (GA) for optimization of the most influencing process variables. A full factorial central composite experimental design was used to study the cumulative interactive effect of nutritional components viz...
October 11, 2017: Preparative Biochemistry & Biotechnology
Sug-Young Yoon, Hyung-Min Lee, Ji-Na Kong, Kwang-Hoon Kong
Agarase catalyzes the hydrolysis of agar, which is primarily used as a medium for microbiology, various food additives, and new biomass materials. In this study, we described the expression of the synthetic gene encoding β-agarase from Agarivorans albus in Escherichia coli. The synthetic β-agarase gene was designed based on the biased codons of E. coli in order to optimize its expression and extracellular secretion in an active, soluble form. The synthesized agarase gene, including its signal sequence, was cloned into the pET-26 expression vector, and the pET-Aaβ-agarase plasmid was introduced into E...
October 11, 2017: Preparative Biochemistry & Biotechnology
B Vaishnavi, C Subathra Devi
Streptokinase purified from Streptococcus equinus VIT_VB2 isolated from bovine milk sample was immobilized in various solid supports namely entrapment in agarose gel, calcium alginate beads and gelatin gel by crosslinking with formaldehyde. Immobilization of streptokinase in calcium alginate beads showed maximum efficiency (81.8 ± 1.06%) when compared to entrapment with agarose gel (55.6 ± 2.17%) and cross-linked gelatin formaldehyde gel (71.0 ± 1.54%). The purified SK activity was expressed maximum in calcium alginate (1%) and gelatin gel (0...
October 11, 2017: Preparative Biochemistry & Biotechnology
Igor G Morgunov, Olga V Karpukhina, Svetlana V Kamzolova, Vladimir A Samoilenko, Anatoly N Inozemtsev
The effect of biologically active form (threo-Ds-) of isocitric acid on oxidative stress was studied using the infusorian Paramecium caudatum stressed by hydrogen peroxide and salts of some heavy metals (Cu, Pb, Zn, and Cd). Isocitric acid at concentrations between 0.5 and 10 mM favorably influenced the infusorian cells with oxidative stress induced by the toxicants studied. The maximal antioxidant effect of isocitric acid was observed at its concentration 10 mM irrespective of the toxicant used (either H2O2 or heavy metal ions)...
October 4, 2017: Preparative Biochemistry & Biotechnology
Shweta V Pawar, Virendra K Rathod
This study explores a novel concept of co-production of uricase and alkaline protease by Bacillus licheniformis using single substrate in single step. Seven local bacterial strains were screened for uricase production amongst which Bacillus licheniformis is found to produce highest uricase along with alkaline protease. Optimization of various factors influencing maximum enzyme co-production by Bacillus licheniformis are investigated. Maximum enzyme productivity of 0.386 U/mL uricase and 0.507 U/mL alkaline protease is obtained at 8 h incubation period, 1% (v/v) inoculum and at 0...
October 4, 2017: Preparative Biochemistry & Biotechnology
Manish G Yadav, Monali R Kavadia, Rajeshkumar N Vadgama, Annamma A Odaneth, Arvind M Lali
Enzymatic synthesis of GMU was carried out using indigenously immobilized Candida anatarctica lipase B preparation (named as PyCal) using glycerol and undecylenic acid as substrates. Effect of molar ratio, enzyme load, reaction time, and organic solvent on the reaction conversion was determined. Both batch and continuous processes for GMU synthesis with shortened reaction time were developed. Under optimized batch reaction conditions such as 1:5 molar ratio of undecylenic acid and glycerol, 2 h reaction time at 30% substrate concentration in tert butyl alcohol, conversion of 82% in absence of molecular sieve and conversion of 93% in presence of molecular sieve was achieved...
October 4, 2017: Preparative Biochemistry & Biotechnology
Vijita V Pillai, Gunjan Prakash, Arvind M Lali
Propionic acid production from glucose was studied using Propionibacterium freudenreichii shermanii. Conditions were optimized for high yields of propionic acid and total organic acids by sequential optimization of parameters like pH, inoculum age, inoculum volume, and substrate concentration. Near theoretical yield (0.54 ± 0.023 g/ g) was achieved for propionic acid with fermentation of 1% glucose using 20% (v/v) of 48 hrs old P.shermanii at 30°C, pH maintained at 5.5. Total organic acid yield under these conditions was 0...
October 4, 2017: Preparative Biochemistry & Biotechnology
Amanda Reges de Sena, Flávio Manoel Barros Oliveira, Tonny Cley Campos Leite, Talita Camila Evaristo da Silva Nascimento, Keila Aparecida Moreira, Sandra Aparecida de Assis
The aims of the current study are to assess the influence of polyethylene glycol (PEG) concentration, molar mass, pH, and citrate concentrations on aqueous biphasic systems based on 2(4) factorial designs, as well as to check their capacity to purify tannase secreted by Aspergillus tamarii URM 7115. Tannase was produced through submerged fermentation at 26°C for 67 h in Czapeck-Dox modified broth and added with yeast extract and tannic acid. The factorial design was followed to assess the influence of PEG molar mass (MPEG 600; 4,000 and 8,000 g/ mol), and PEG (CPEG 20...
August 31, 2017: Preparative Biochemistry & Biotechnology
Juan Daniel Rivaldi, Ana Karine F Carvalho, Leyvison Rafael V da Conceição, Heizir F de Castro
Increased costs and limited availability of traditional lipid sources for biodiesel production encourage researchers to find more sustainable feedstock at low prices. Microbial lipid stands out as feedstock replacement for vegetable oil to convert fatty acid esters. In this study, the potential of three isolates of filamentous fungi (Mucor circinelloides URM 4140, M. hiemalis URM 4144, and Penicillium citrinum URM 4126) has been assessed as single-cell oil (SCO) producers. M. circinelloides 4140 had the highest biomass concentration with lipid accumulation of up to 28 wt% at 120 hr of cultivation...
August 31, 2017: Preparative Biochemistry & Biotechnology
Thomas Langer, Carsten Corvey, Katja Kroll, Oliver Boscheinen, Thomas Wendrich, Werner Dittrich
Glycosylation is one of the most complex post-translational modifications and may have significant influence on the proper function of the corresponding proteins. Bacteria and yeast are, because of easy handling and cost reasons, the most frequently used systems for recombinant protein expression. Bacteria generally do not glycosylate proteins and yeast might tend to hyperglycosylate. Insect cell- and mammalian cell-based expression systems are able to produce complex N-glycosylation structures but are more complex to handle and more expensive...
August 31, 2017: Preparative Biochemistry & Biotechnology
Ali Osman Adıgüzel, Münir Tunçer
A total of approximately 400 bacterial strains were isolated from 73 plastic wastes collected from 14 different regions. Nineteen isolates that form clear zones both on tributyrin and poly ε-caprolactone (PCL) agar, were identified based on 16S rRNA gene sequences. Among these, Bacillus sp. KY0701 that caused the highest weight loss of PCL films in minimal salt medium, was selected for cutinase production. The highest enzyme activity (15 U/mL) was obtained after 4 days of incubation at 50°C, pH 7.0 and 200 rpm in a liquid medium containing 1...
August 31, 2017: Preparative Biochemistry & Biotechnology
Mansoureh Fazelahi, Vahid Kia, Hooman Kaghazian, Mahdi Paryan
Recombinant streptokinase is a biopharmaceutical which is usually produced in E. coli. Residual DNA as a contamination and risk factor may remain in the product. It is necessary to control the production procedure to exclude any possible contamination. The aim of the present study was to develop a highly specific and sensitive quantitative real-time PCR-based method to determine the amount of E. coli DNA in recombinant streptokinase. A specific primers and a probe was designed to detect all strains of E. coli...
August 31, 2017: Preparative Biochemistry & Biotechnology
Hooi Xian Lee, Fisal Ahmad, Bahruddin Saad, Mohd Nazri Ismail
Date fruits are well known to be very nutritious. Nevertheless, the protein contents of the fruit, particularly the seed and flesh, are still understudied, largely due to their difficult physical characteristics. This study was conducted to compare three different protein extraction methods which were the trichloroacetic acid (TCA)-acetone (TCA-A), phenol (Phe), and TCA-acetone-phenol (TCA-A-Phe), and to perform proteomic analysis on date palm seed and flesh. Phe extraction method showed the highest protein yields for both seed (8...
August 31, 2017: Preparative Biochemistry & Biotechnology
Mu-Tan Luo, Chao Huang, Xue-Fang Chen, Qian-Lin Huang, Gao-Xiang Qi, Lan-Lan Tian, Lian Xiong, Hai-Long Li, Xin-De Chen
Biomass acid hydrolysate of oleaginous yeast Trichosporon cutaneum after microbial oil extraction was applied as substrate for bacterial cellulose (BC) production by Komagataeibacter xylinus (also named as Gluconacetobacter xylinus previously) for the first time. BC was synthesized in static culture for ten days and the maximum BC yield (2.9 g/ L) was got at the 4th day of fermentation. Most carbon sources in the substrate (glucose, mannose, formic acid, acetic acid, etc.) can be utilized by K. xylinus...
August 31, 2017: Preparative Biochemistry & Biotechnology
Ngangoum Eric Serge, Mezajoug Kenfack Laurette Blandine, Sanjit Kumar, Tchiégang Clergé, Mookambeswaran Vijayalakshmi
A protease from fresh leaves of Abrus precatorius was purified using two classical chromatography techniques: ion exchange (DEAE-Sepharose) and Gel filtration (Sephadex G-75). The Purified protease showed a molecular weight of ∼ 28 kDa on sodium dodecyl gel electrophoresis (SDS-PAGE).The optimum pH and temperature for the purified protease was 8 and 40°C respectively. The purified protease was stable throughout a wide temperature range from 10°C to 80°C and pH from 2 to 12. Protease activity was inhibited in presence of Co(2+), Ni(2+), Hg(2+) and Zn(2+) while its activity has increased in presence of Ca(2+) and Mg(2+)...
August 31, 2017: Preparative Biochemistry & Biotechnology
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