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Preparative Biochemistry & Biotechnology

Dario Rafael Olicón-Hernández, Pedro Alberto Vázquez-Landaverde, Ramón Cruz-Camarillo, Luz Irene Rojas-Avelizapa
Bacillus thuringiensis is a non-human pathogen bacterium that is used as a fungal and insect biocontrol agent. Because of its environmental interaction, it possesses several extracellular enzymes that are able to degrade chitin and chitosan, two of the most important polymers because of their application in numerous fields. However, in recent years, it has been observed that oligosaccharides from the enzymatic degradation of chitosan have important benefits for human health. Compare and explore the production of chitooligosaccharides from different sources of chitosan will improve the process parameters and expand the biotechnology based in this molecules...
November 10, 2016: Preparative Biochemistry & Biotechnology
Sepideh Khazeni, Ashrafalsadat Hatamian-Zarmi, Fatemeh Yazdian, Zahra Beagom Mokhtari-Hosseini, Bahman Ebrahimi-Hosseinzadeh, Behnam Noorani, Ghassem Amoabedini, Mohammad Reza Soudi
Bacterial Cellulose (BC) is a very fascinating microbial biopolymer which is mainly produced by Gluconacetobacter xylinum. Optimization of BC production by G. xylinum was carried out based on scale-down studies in miniature-bioreactor and response surface methodology in which the optimum pH value (6.5) and shaking rate (50 rpm) were obtained. The static culture condition for BC production has newly been defined. Nano-structure of BC includes nano-fibers up to (60 nm) and nano-porosity up to (265 nm) was observed by SEM...
November 8, 2016: Preparative Biochemistry & Biotechnology
Hanen Mallek-Fakhfakh, Jawhar Fakhfakh, Najla Masmoudi, Fatma Rezgui, Ali Gargouri, Hafedh Belghith
In the present study we investigated a potent extracellular β-glucosidases secreted by the thermophilic fungal strain AX4 of Talaromyces thermophilus, isolated from Tunisian soil samples. This strain was selected referring to the highest thermostability of its β-glucosidases compared to the other fungal isolates. The β-glucosidase production was investigated by submerged fermentation (SmF). The optimal temperature and initial pH for maximum β-glucosidase production were 50°C and 7.0, respectively. Several carbon sources were assayed for their effects on β-glucosidase production, significant yields were obtained in media containing lactose 1% (3...
November 8, 2016: Preparative Biochemistry & Biotechnology
Siriporn Chaikaew, Apinun Kanpiengjai, Jenjira Intatep, Kridsada Unban, Goro Takata, Chartchai Khanongnuch
The present study demonstrates the effectiveness of x-ray radiation in strain improvement for defective lipase production by Bacillus sp. MR10 for further application in the fermentative production of manno-oligosaccharides (MOS) from agricultural by-product, defatted copra meal (DCM). The mutants obtained were screened based on their defective lipase activity together with their β-mannanase production performance. Among ten selected mutants, the strain M7 was the highest promising mutant regarding the smallest lipase activity (0...
November 7, 2016: Preparative Biochemistry & Biotechnology
Ashish A Prabhu, A Jayadeep
The current study is focused on optimizing the parameters involved in enzymatic processing of red rice bran for maximizing total polyphenol (TP) and free radical scavenging activity (FRSA). The sequential optimization strategies using central composite design (CCD) and artificial neural network (ANN) modeling linked with Genetic algorithm (GA) was carried out to study the effect of incubation time (60-90 min), xylanase concentration (5-10 mg/g), cellulase concentration (5-10 mg/g) on the response i.e., total polyphenol and FRSA...
November 4, 2016: Preparative Biochemistry & Biotechnology
Iszatty Ismail, Tana Poorani Gurusamy, Hema Ramachandran, Amirul Al-Ashraf Abdullah
Anti-biofilm polymers have the ability to inhibit bacterial biofilm formation, which is known to occur ubiquitously in the environment and pose risks of infection. In this study, production of P(3HB-co-4HB) copolymer and antimicrobial yellow pigment from Cupriavidus sp. USMAHM13 are enhanced via medium optimization. Prior to the improvement of yellow pigment production, screening for the best additional supplement was performed resulting in high-yield yellow pigmentation using yeast extract with optimum concentration of 2 g/L...
November 4, 2016: Preparative Biochemistry & Biotechnology
Moolchand Sigar, Nitu Maity, Saroj Mishra
Protein fusion technology has emerged as one of the important strategies to increase the level of expression and half-life of therapeutic proteins in heterologous expression systems. G-CSF (granulocyte-colony stimulating factor) is a hematopoietic growth factor and is clinically used against neutropenia. Enhanced expression and stability of G-CSF was achieved in Pichia pastoris by way of constructing a fusion protein with Human Serum Albumin (HSA). The strategy involved PCR amplification of fragments corresponding to codon-optimized G-CSF and domain 3 of HSA...
November 4, 2016: Preparative Biochemistry & Biotechnology
Habibollah Faraji, Mohammad Ramezani, Hamid Reza Sadeghnia, Khalil Abnous, Fatemeh Soltani, Baratali Mashkani
Staphylokinase (SAK) as the third generation thrombolytic molecule is a promising agent for the treatment of thrombosis. SAK variant of SAKфC was expressed in Pichia pastoris strains KM71H and GS115. The codon adaptation index of SAK was improved from 0.75 to 0.89. The expression of recombinant SAK (rSAK) reached to its maximum (310 mg/L of the culture medium) after 48 h stimulation with 3% methanol and remained steady until day 5. The maximum activity of the enzyme was at pH 8.6 and 37°C. It was highly active at temperatures 20-37°C and pH ranges of 6...
November 4, 2016: Preparative Biochemistry & Biotechnology
Pouria Gholami Tilko, Zahra Hajihassan, Hamid Moghimi
Human nerve growth factor (hNGF) a member of the neurotrophin family can be used to treat neurodegenerative diseases. As it has disulfide bonds in its structure, periplasmic expression of it using appropriate signal sequence is beneficial. Therefore, in this work β-NGF was expressed in E. coli using pET39b expression vector containing dsbA signal sequence. In an initial step, the effect of IPTG and lactose concentration as inducer on protein production was investigated using response surface methodology (RSM)...
November 4, 2016: Preparative Biochemistry & Biotechnology
V Gohel, K Ranganathan, G Duan
Conventional grain ethanol manufacturing is a high temperature energy intensive process comprising of multiple unit operations when combined with lower ethanol recovery results in higher production cost. In liquefaction, jet cooking accounts for significant energy cost while strong acid or base used for pH adjustment presents a safety hazard. A need is felt for sustainable ethanol manufacturing process that is less hazardous, consumes lower energy and operates in a low pH range of 4.50 to 5.50. A Single Temperature Liquefaction (STL) process that could efficiently operate at lower liquefaction temperature over a pH range of 4...
October 13, 2016: Preparative Biochemistry & Biotechnology
Eun Jae Kim, Jun Hyuck Lee, Sung Gu Lee, Se Jong Han
To survive in a sub-zero environment, polar organisms produce ice-binding proteins (IBPs). These IBPs prevent the formation of large intracellular ice crystals, which may be fatal to the organism. Recently, a recombinant FfIBP (an IBP from Flavobacterium frigoris PS1) was cloned and produced in Pichia pastoris using fed-batch fermentation with methanol feeding. In this study, we demonstrate that FfIBP produced by P. pastoris has a glycosylation site, which diminishes the thermal hysteresis (TH) activity of FfIBP...
October 13, 2016: Preparative Biochemistry & Biotechnology
K Divakar, M Suryia Prabha, G Nandhinidevi, P Gautam
The simultaneous production of intracellular esterase and extracellular protease from the strain Lysinibacillus fusiformis AU01 was studied in detail. The production was performed both under batch and fed-batch modes. The maximum yield of intracellular esterase and protease was obtained under full oxygen saturation at the beginning of the fermentation. The data were fitted to the Luedeking-Piret model and it was shown that the enzyme (both esterase and protease) production was growth associated. A decrease in intracellular esterase and increase in the extracellular esterase were observed during late stationary phase...
October 13, 2016: Preparative Biochemistry & Biotechnology
Yan-Yang Xu, Yang Qiu, Hui Ren, Dong-Hu Ju, Hong-Lei Jia
Aronia melanocarpa berries are abundant in polyphenolic compounds. After juice production, the pomace of pressed berries still contains a substantial amount of polyphenolic compounds. For efficient utilization of Aronia melanocarpa berries and the enhancement of polyphenolic compounds yields in Aronia melanocarpa pomace (AMP), total phenolics (TP) and total flavonoids (TF) from AMP were extracted, using ultrasound-assisted aqueous two-phase system (UAE-ATPS) extraction method. Firstly, the influences of ammonium sulfate concentration, ethanol-water ratio, ultrasonic time and ultrasonic power on TP and TF yields were investigated...
October 13, 2016: Preparative Biochemistry & Biotechnology
Paran Gani, Norshuhaila Mohamed Sunar, Hazel Matias-Peralta, Ab Aziz Abdul Latiff, Siti Fatimah Zaharah Mohamad Fuzi
Microalgae biomass has been recognised as a sustainable bio-product to replace terrestrial biomass in biofuel production. The microalgae industry has high operating costs, specifically on harvesting and biomass recovery. Therefore, the development of an efficient harvesting method is crucial to the minimisation of production cost. A statistical analysis via response surface methodology was used to investigate the optimisation of harvesting efficiency using alum and chitosan as a coagulant. Growth rate and biomass productivity were also determined...
October 13, 2016: Preparative Biochemistry & Biotechnology
Aleksandra Margetić, Zoran Vujčić
Yeast Saccharomyces cerevisiae is the most significant source of enzyme invertase. It is mainly used in the food industry as a soluble or immobilized enzyme. The greatest amount of invertase is located in the periplasmic space in yeast. In this work it was isolated two forms of enzyme from yeastS. cerevisiae cell, soluble and cell wall invertase (CWI). Both forms of enzyme showed same temperature optimum (60°C), similar pH optimum and kinetic parameters. The significant difference between these biocatalysts was observed in their thermal stability, stability in urea and methanol solution...
October 13, 2016: Preparative Biochemistry & Biotechnology
Ahmed Abd Elrazak, Alan C Ward, Jarka Glassey
The increase demand of Omega-3 in the market and the challenges facing its conventional supplies led to an increase interest to microbial Omega-3 sources. This research concentrates on the statistical role of some metal ions on the biosynthesis and productivity of eicosapentanoic acid (essential omega-3 element) in bacterial isolate, Shewanella 717. A Plackett-Burman design was applied to screen the main effect of all metal salts entrenched in the Artifical Sea Water medium components. Four salts in particular in addition to the interaction among them were highlighted as having a statistically significant effect upon the growth and/or EPA production...
October 13, 2016: Preparative Biochemistry & Biotechnology
Sumitra Datta, Gopalakrishnan Menon, Bincy Varughese
Proteolytic Aeromonas caviae P-1-1 growing at wide ranging pH (7.0-11.0) and moderate salinity (0-5% NaCl) was isolated from cattle-shed of Thanjavur, India. It produced lipase, gelatinase and polyhydroxybutyrate. Different culture conditions, incubation time, carbon and nitrogen sources, vitamins, amino acids, surfactants and metal ions for optimal growth and protease production of P-1-1 were examined. Maximum protease (0.128 U/mL) production was achieved with 1% fructose, 1% yeast extract, 0.1% ammonium sulfate, 3% NaCl, 0...
October 13, 2016: Preparative Biochemistry & Biotechnology
Karla Silva Teixeira Souza, Cintia Lacerda Ramos, Rosane Freitas Schwan, Disney Ribeiro Dias
The main carbon source used for growth by four yeast strains (Yarrowia lipolytica CCMA 0357, Y. lipolytica CCMA 0242, Wickerhamomyces anomalus CCMA 0358, and Cryptococcus humicola CCMA 0346) and their lipid production were evaluated, using different concentrations of crude and pure glycerol and glucose. Whereas crude glycerol (100 g/L) was the main carbon source used by Y. lipolytica CCMA 0357 (nearly 15 g/L consumed at 120 h) and W. anomalus CCMA 0358 (nearly 45.10 g/L consumed at 48 h), pure glycerol (150 g/L) was the main one used by C...
October 13, 2016: Preparative Biochemistry & Biotechnology
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November 16, 2016: Preparative Biochemistry & Biotechnology
Kunal Kumar, Prasanna D Belur
Oxalate oxidase (EC catalyzes the oxidative cleavage of oxalate to carbon dioxide with the reduction of molecular oxygen to hydrogen peroxide. Oxalate oxidase found its application in clinical assay for oxalate in blood and urine. This study describes the purification and biochemical characterization of an oxalate oxidase produced from an endophytic bacterium, Ochrobactrum intermedium CL6. The cell-free fermentation broth was subjected to two-step enzyme purification, which resulted in a 58.74-fold purification with 83% recovery...
October 2, 2016: Preparative Biochemistry & Biotechnology
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