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Preparative Biochemistry & Biotechnology

Sumitha Banu Jamaldheen, Kedar Sharma, Aruna Rani, Vijayanand S Moholkar, Arun Goyal
This study compares different types of pretreatment methods, such as thermal pretreatment at 120°C, autoclaving, microwaving and ultrasonication in the presence of water, dilute acid (1% H2 SO4 ) or dilute alkali (1% NaOH) on Sorghum stalk with respect to the holocellulose and ADL (Acid Detergent/Insoluble Lignin) content. Among all the methods, pretreatment with 1% NaOH along with autoclaving at 121°C and 15 psi for 30 min was the most effective method for Sorghum stalk. FTIR analysis of this pretreated biomass showed the removal of lignin and FESEM analysis displayed enhanced surface roughness...
May 10, 2018: Preparative Biochemistry & Biotechnology
Nitin Kumar, Rajat Pandey, Ashish Anand Prabhu, Veeranki Venkata Dasu
Human interferon-gamma (hIFNG) production is limited by various gene level bottle-necks including translation, protein folding and secretion which depends upon the physiological state of the organism. In this study gene-level and substrate-level modulations have been used to control Bacillus subtilis physiology for >10.39 fold extracellular soluble hIFNG production. Two variants of the native human interferon-gamma gene (hifng) were designed and synthesized namely BScoIFNGhis and BScoIFNG having codon adaptation index 25...
April 24, 2018: Preparative Biochemistry & Biotechnology
Zichao Wang, Libo Yang, Jianrong Wu, Hongtao Zhang, Li Zhu, Xiaobei Zhan
Xanthan gum is commonly used as thickener in food industry, while the usage of xanthan gum as dietary fiber is restricted for its low additive volume. Herein, the potential use of a low-viscosity and high-transparency xanthan gum as dietary fiber was evaluated in vitro . This new xanthan shows better transparency and faster dissolution rate than most commercial products, and its viscosity increases along with the treatment of freeze-thaw cycles at -20 °C. Moreover, this new xanthan can absorb heavy metals (Pb, Cd, Cu) and retard starch digestion by glucoamylase...
March 22, 2018: Preparative Biochemistry & Biotechnology
Shenghao Wang, Muhammad Kalim, Keying Liang, Jinbiao Zhan
Glypican-3 (GPC3) is an integral membrane proteoglycan, which contains a core protein anchored to the cytoplasmic membrane through a glycosyl phosphatidylinositol linkage. The glypican-3 can regulate the signaling pathways, thereby enhances cell division, growth, and apoptosis in certain cell types. It is almost non-existent on the surface of the human normal cell membrane and highly expresses on the membrane of hepatocellular carcinoma (HCC) cells. It has been well established that GPC3 provide a useful diagnostic marker...
March 21, 2018: Preparative Biochemistry & Biotechnology
Jin Huang, Wan Tang, Shengquan Zhu, Meini Du
Butyric acid (C3 H7 COOH) is an important chemical that is widely used in foodstuffs along with in the chemical and pharmaceutical industries. The bioproduction of butyric acid via large-scale fermentation has the potential to be more economical and efficient than petrochemical synthesis. In this paper, the metabolic pathways involved in the production of butyric acid from Clostridium tyrobutyricum using hexose and pentose as substrates are investigated, and approaches to enhance butyric acid production via genetic modification are discussed...
March 21, 2018: Preparative Biochemistry & Biotechnology
Xia Liu, Yongdong Xu, Zhi Li, Shengwei Jiang, Shuo Yao, Rina Wu, Yingfeng An
A silica sands-based method has been developed to isolate high quality genomic DNAs from cells of animals, plants and microorganisms, such as Hemisalanx prognathus, Spinacia oleracea, Pichia pastoris, Bacillus licheniformis and Escherichia coli. To the best of our knowledge, no DNA isolation method has so wide application until now. In addition, this method and a commercially available kit were compared in analysis of microbial communities using high-throughput 16 s rDNA sequencing. As a result, the silica sands-based method was found to be even more efficient in isolating genomic DNA from gram-positive bacteria than the kit, indicating that it would become a very valuable choice to faithfully reflect the composition of microbial communities...
March 21, 2018: Preparative Biochemistry & Biotechnology
S Prakash, K Rajeswari, P Divya, M Ferlin, C T Rajeshwari, B Vanavil
Curdlan gum is a neutral water-insoluble bacterial exopolysaccharide composed primarily of linear β-(1,3) glycosidic linkages. Recently, there has been increasing interest in the applications of curdlan and its derivatives. Curdlan is found to inhibit tumors and its sulfated derivative possess anti- HIV activity. Curdlan is biodegradable, non-toxic towards human, environment and edible which makes it suitable as drug-delivery vehicles for sustained drug release. The increasing demand for the growing applications of curdlan requires an efficient high yield fermentation production process so as to satisfy the industrial needs...
March 21, 2018: Preparative Biochemistry & Biotechnology
Nihan Arabacı, Burhan Arıkan
A cold-active alkaline amylase producer Bacillus subtilis N8 was isolated from soil samples. Amylase synthesis optimally occurred at 15°C and pH 10.0 on agar plates containing starch. The molecular weight of the enzyme was found to be 205 kDa by performing SDS-PAGE. While the enzyme exhibited the highest activity at 25°C and pH 8.0, it was highly stable in alkaline media (pH 8.0-12.0) and retained 96% of its original activity at low temperatures (10-40°C) for 24 h. While the amylase activity increased in the presence of β-mercaptoethanol (103%); Ba2+ , Ca2+ , Na+ , Zn2+ , Mn2+ , H2 O2 and TritonX-100 slightly inhibited the activity...
March 21, 2018: Preparative Biochemistry & Biotechnology
Xiao-Yan Wang, Hui Ren
Ginseng stems and leaves (GSAL) are abundant in ginsenosides compounds. For efficient utilization of GSAL and the enhancement of total ginsenosides (TG) compound yields in GSAL, TG from GSAL were extracted, using dynamic-microwave assisted extraction coupled with enzymatic hydrolysis (DMAE-EH) method. The extraction process has been simulated and its main influencing factors such as ethanol concentration, microwave temperature, microwave time and pump flow rate have been optimized by response surface methodology coupled with a Box-Behnken design(BBD)...
March 21, 2018: Preparative Biochemistry & Biotechnology
Ying Liang, Li Zhu, Minjie Gao, Jianrong Wu, Xiaobei Zhan
Water-soluble β-1,3-glucan (w-glucan) prepared from curdlan is reported to possess various bioactive and medicinal properties. To develop an efficient and cost-effective microbial fermentation method for the direct production of w-glucan, a coupled fermentation system of Agrobacterium sp. and Trichoderma harzianum (CFS-AT) was established. The effects of Tween-80, glucose flow rate, and the use of a dissolved oxygen (DO) control strategy on w-glucan production were assessed. The addition of 10 g L-1 Tween-80 to the CFS-AT enhanced w-glucan production, presumably by loosening the curdlan ultrastructure and increasing the efficiency of curdlan hydrolysis...
May 28, 2018: Preparative Biochemistry & Biotechnology
Mark-Henry Kamga, Maurizio Cattaneo, Seongkyu Yoon
A new integrated continuous biomanufacturing platform for continuous production of antibodies at fixed cell volumes and cell concentrations for extended periods with immediate capture is presented. Upstream antibody production has reached technological maturity, however, the bottleneck for continuous biomanufacturing remains the efficient and cost-effective capture of therapeutic antibodies in an initial chromatography step. In this study, the first successful attempt at using one-column continuous chromatography (OCC) for the continuous capture of therapeutic antibodies produced through alternating tangential flow perfusion is presented...
May 28, 2018: Preparative Biochemistry & Biotechnology
Thanaporn Wannachod, Sutthinee Wannasutthiwat, Sorawit Powtongsook, Kasidit Nootong
Photoautotrophic cultivation of Chlorococcum humicola was performed in batch and continuous modes in different cultivating system arrangements to compare biomass and carotenoids' concentration and their productivities. Batch result from stirred tank and airlift photobioreactors indicated the positive effect of increasing light intensity on growth and carotenoid production, whereas the finding from continuous cultivation indicated that carotenoid enhancement preferred high light intensity and nitrogen-deficient environment...
April 21, 2018: Preparative Biochemistry & Biotechnology
Shruthi Sunderrajan, Lima Rose Miranda, Gautam Pennathur
Graphene oxide/chitosan and reduced graphene oxide/chitosan (GO/CS and RGO/CS) beads were prepared by precipitation with NaOH. Porcine liver esterase was immobilized on these beads to give GO/CS/E and RGO/CS/E beads. The optimum conditions for the maximum activity of RGO/CS/E beads were pH 8 and 50°C. The stability of the enzyme immobilized on GO/CS/E and RGO/CS/E was high in the pH range of 5-8. The GO/CS/E beads showed superior stability compared to that of the free enzyme and CS/E beads between 20 and 50°C...
April 21, 2018: Preparative Biochemistry & Biotechnology
Davood Mazaheri, Seyed Abbas Shojaosadati, Seyed Morteza Zamir, Seyyed Mohammad Mousavi
In this work, mathematical modeling of ethanol production in solid-state fermentation (SSF) has been done based on the variation in the dry weight of solid medium. This method was previously used for mathematical modeling of enzyme production; however, the model should be modified to predict the production of a volatile compound like ethanol. The experimental results of bioethanol production from the mixture of carob pods and wheat bran by Zymomonas mobilis in SSF were used for the model validation. Exponential and logistic kinetic models were used for modeling the growth of microorganism...
April 21, 2018: Preparative Biochemistry & Biotechnology
Thanongsak Chaiyaso, Wimada Srisuwan, Charin Techapun, Masanori Watanabe, Shinji Takenaka
The new amylolytic oleaginous red yeast, Sporidiobolus pararoseus KX709872, produced both α-amylase (540 ± 0.09 mU/mL) and amyloglucosidase (23 ± 0.00 mU/mL) and showed good ability to directly convert rice residue from canteen waste to biomass and lipids. Effects of medium composition and cultivation conditions on growth and lipid accumulation for strain KX709872 were investigated under shaking flask and upscaling levels. At C : N ratio of 25 : 1, pH 5.45, 22.36°C, and 199.40 rpm for 7 days, volumetric production of biomass and lipids, lipid content, and lipid productivity reached 17...
April 21, 2018: Preparative Biochemistry & Biotechnology
Mingcong Shao, Lili Xiu, Haijiang Zhang, Jianying Huang, Xingwen Gong
Chitosan/cellulose-based beads (CCBs) for the affinity purification of histidine-tagged proteins were prepared from chitosan/cellulose dissolved in ionic liquid as a solvent, and their structures were characterized by Fourier transform infrared spectroscopy, transmission electron microscopy, and thermogravimetric analysis. The affinity purification was used to separate hexahistidine-tagged (his-tagged) enhanced green fluorescent protein (EGFP) from Escherichia coli. The results showed that Zn2+ -CCB exhibited more specific adsorption capacity toward the target protein compared with Ni2+ -CCB and Cu2+ -CCB...
April 21, 2018: Preparative Biochemistry & Biotechnology
M Mervan Çakar, Juan Mangas-Sanchez, William R Birmingham, Nicholas J Turner, Barış Binay
Over the next decades, with the growing concern of rising atmospheric carbon dioxide (CO2 ) levels, the importance of investigating new approaches for its reduction becomes crucial. Reclamation of CO2 for conversion into biofuels represents an alternative and attractive production method that has been studied in recent years, now with enzymatic methods gaining more attention. Formate dehydrogenases (FDHs) are NAD(P)H-dependent oxidoreductases that catalyze the conversion of formate into CO2 and have been extensively used for cofactor recycling in chemoenzymatic processes...
April 21, 2018: Preparative Biochemistry & Biotechnology
Aungkana Srisuwun, Nidtaya Tantiwa, Ampin Kuntiya, Arthitaya Kawee-Ai, Apisit Manassa, Charin Techapun, Phisit Seesuriyachan
This experiment aimed to decolorize Reactive Red 159 using a high potential of a consortium of purple nonsulfur bacteria (PNSB) with an application of response surface methodology through a central composite design in open system. The three factors of hydraulic retention time (HRT), sludge retention time (SRT) and dye concentration were applied to the design. The decolorization was operated in an anaerobic sequencing batch reactor until the system reached to a pseudosteady state for 30 cycles in each experiment...
April 21, 2018: Preparative Biochemistry & Biotechnology
Rajesh Kumar Sahoo, Mohit Kumar, Swati Mohanty, Matthew Sawyer, Pattanathu K S M Rahman, Lala Behari Sukla, Enketeswara Subudhi
The production of biofuel using thermostable bacterial lipase from hot spring bacteria out of low-cost agricultural residue olive oil cake is reported in the present paper. Using a lipase enzyme from Bacillus licheniformis, a 66.5% yield of methyl esters was obtained. Optimum parameters were determined, with maximum production of lipase at a pH of 8.2, temperature 50.8°C, moisture content of 55.7%, and biosurfactant content of 1.693 mg. The contour plots and 3D surface responses depict the significant interaction of pH and moisture content with biosurfactant during lipase production...
April 21, 2018: Preparative Biochemistry & Biotechnology
Sandeep Shewale, Virendra K Rathod
The objective of this work is to put forth the optimization and kinetics of total phenolic compounds extraction from Azadirachta indica leaves in a stirred batch extraction. Various experiential extraction parameters have been studied for maximum extraction of the total phenolic compounds. The maximum yield of total phenolic compounds was found to be 10.80 mg g-1 of dried neem powder under the optimized conditions. The extraction kinetics behavior followed first-order kinetics with diffusion coefficient ranged from 1...
April 21, 2018: Preparative Biochemistry & Biotechnology
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