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Preparative Biochemistry & Biotechnology

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https://www.readbyqxmd.com/read/28718734/pullulan-production-from-synthetic-medium-by-a-new-mutant-of-aureobasidium-pullulans
#1
Guoqiang Chen, Jiming Wang, Yulong Su, Youshuang Zhu, Ge Zhang, Hongwei Zhao, Haobao Liu, Ying Yang, Rui Nian, Haibo Zhang, Yuxi Wei, Mo Xian
Pullulan with different molecular-weight could be applied in various fields. A UV-induced mutagenesis Aureobasidium pullulans UVMU6-1 was obtained from the strain A. pullulans CGMCC3.933 for the production of low-molecular-weight pullulan. Firstly, the obtained polysaccharide from A. pullulans UVMU6-1 was purified and identified to be pullulan with TLC, FT-IR and NMR. Then, culture medium and conditions for this strain were optimized by flask fermentation. Based on the optimized medium and culture conditions (pH 4, addition of 4 g/L Tween 80 for 96 h of cultivation), continuously fermentation was performed...
July 18, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28708469/aqueous-two-phase-assisted-by-ultrasound-for-the-extraction-of-anthocyanins-from-lycium-ruthenicum-murr
#2
Benlin Qin, Xuecong Liu, Haiming Cui, Yue Ma, Zimin Wang, Jing Han
In this study, an efficient ultrasound-assisted aqueous two-phase extraction (UA-ATPE) method was employed for the extraction of anthocyanins from Lycium ruthenicum Murr(LRMAs). An ethanol/ammonium sulfate system was chosen for the aqueous two-phase system (ATPS) due to its fine partitioning and recycling behaviors. Single-factor experiments were conducted to determine the optimized composition of the system, and the response surface methodology (RSM) was used for the further optimization of the ultrasound-assisted aqueous two-phase extraction...
July 14, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28708454/improving-the-production-of-s-adenosyl-l-methionine-in-escherichia-coli-by-overexpressing-metk
#3
Ping Yu, Pengzhi Zhu
S-adenosyl-L-methionine (SAM) has important applications in many fields including the chemical therapy and the pharmaceutical industry. In this study, the recombinant Escherichia coli strain was constructed for effective production of SAM by introducing the SAM synthase gene (metK). This strain produced 34.5 mg/l of SAM in basic medium in shake flask. Yeast extract, pH and loaded volume had a significant positive effect on the yield of SAM. Their optimal values were 35 g/l, 7.5 and 30 ml, respectively...
July 14, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28662360/adsorption-kinetics-isotherm-and-thermodynamics-studies-of-acetyl-11-keto-%C3%AE-boswellic-acids-akba-from-boswellia-serrata-extract-using-macroporous-resin
#4
Sonali S Niphadkar, Virendra K Rathod
An acetyl 11-keto-β-boswellic acid (AKBA) is potent anti-inflammatory agent found in Boswellia serrata oleogum resin. Adsorption characteristics of AKBA from Boswellia serrata were studied using macroporous adsorbent resin to understand separation and adsorption mechanism of targeted molecules. Different macroporus resins were screened for adsorption and desorption of AKBA and Indion 830 was screened as it showed higher adsorption capacity. The kinetic equations were studied and results showed that the adsorption of AKBA on Indion 830 was well fitted to the Pseudo first-order kinetic model...
June 29, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28644760/expression-and-characterization-of-soybean-seed-coat-peroxidase-in-escherichia-coli-bl21-de3
#5
Changqing Liu, Kai Zheng, Ying Xu, Lacmata Tamekou Stephen, Jiming Wang, Hongwei Zhao, Tongqing Yue, Rui Nian, Haibo Zhang, Mo Xian, Huizhou Liu
Soybean seed coat peroxidase(SBP) is a valuable enzyme having a broad variety of applications in analytical chemistry, biochemistry and food processing. In the present study, the sscp gene (Gene ID: 548068) was optimized based on the preferred codon usage of Escherichia coli, synthesized, and expressed in E. coli BL21(DE3). SDS-PAGE and western blot analysis of this expressed protein revealed that its molecular weight is approximately 39 kDa. The effects of induction temperature, concentration of IPTG (isopropyl-β-D-thiogalactoside) and hemin, induction time, expression time were optimized to enhance SBP production with a maximum activity of 11...
June 23, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28644710/study-of-artemisinin-and-sugars-accumulation-in-artemisia-vulgaris-and-artemisia-dracunculus-hairy-root-cultures
#6
Kateryna O Drobot, Nadiia A Matvieieva, Andriy M Ostapchuk, Maxim A Kharkhota
We studied the effect of genetic transformation on biologically active compounds (artemisinin and its co-products (ART) as well as sugars) accumulation in Artemisia vulgaris and A.dracunculus "hairy" root cultures. Glucose, fructose, sucrose and mannitol were accumulated in A. vulgaris and A. dracunculus "hairy" root lines. Genetic transformation has led in some cases to the sugars content increasing or appearing of non-relevant for the control plants carbohydrates. Sucrose content was 1.6 times higher in A...
June 23, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28636483/lumping-kinetics-of-abe-fermentation-wastewater-treatment-by-oleaginous-yeast-trichosporon-cutaneum
#7
Can Wang, Chao Huang, Xue-Fang Chen, Hai-Rong Zhang, Lian Xiong, Xiao-Mei Li, Hai-Jun Guo, Gao-Xiang Qi, Xiao-Qing Lin, Xin-De Chen
Lumping kinetics models were built for the biological treatment of ABE (acetone-butanol-ethanol) fermentation wastewater by oleaginous yeast Trichosporon cutaneum with different fermentation temperatures. Compared with high temperature (33°C, 306 K) and low temperature (23°C, 296 K), medium temperature (28°C, 301 K) was beneficial for the cell growth and COD degradation during the early stage of fermentation but the final yeast biomass and COD removal were influenced little. By lumping method, the materials in the bioconversion network were divided into five lumps (COD, lipid, polysaccharide, other intracellular products, other extracellular products), and the nine rate constants (k1-k9) for the models can well explain the bioconversion laws...
June 21, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28636478/pathway-and-rate-limiting-step-of-glyphosate-degradation-by-aspergillus-oryzae-a-f02
#8
Gui-Ming Fu, Yan Chen, Ru-Yi Li, Xiao-Qiang Yuan, Cheng-Mei Liu, Bin Li, Yin Wan
Aspergillus oryzae A-F02, a glyphosate-degrading fungus, was isolated from an aeration tank in a pesticide factory. The pathway and rate-limiting step of glyphosate (GP) degradation were investigated through metabolite analysis. GP, aminomethylphosphonic acid (AMPA) and methylamine were detected in the fermentation liquid of A. oryzae A-F02, whereas sarcosine and glycine were not. The pathway of GP degradation in A. oryzae A-F02 was revealed: GP was firstly degraded into AMPA, which was then degraded into methylamine...
June 21, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28636463/optimization-of-an-anti-her2-nanobody-expression-using-the-taguchi-method
#9
Alireza Farasat, Fatemeh Rahbarizadeh, Davoud Ahmadvand, Fatemeh Yazdian
Despite being widely used in immunotherapy of cancer, whole antibodies are limited by several disadvantages. This has led to the advent of novel biomolecules such as nanobodies. Taguchi method is a statistical experimental design to study the effect of multiple variables in biological processes. In an effort to overexpress a recombinant anti-HER2 nanobody, we carried out a detailed study to find optimal condition of temperature, induction, culture media, vector and host strain, using Taguchi methodology. A total of 16 various experiments were designed...
June 21, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28636439/functional-enrichment-of-mannanase-treated-spent-brewer-yeast
#10
Ruge Cao, Xingyue Yang, Wenting Shang, Zhongkai Zhou, Padraig Strappe, Chris Blanchard
In this study, the effect of Bacillus brevis produced β-mannanase on the nutrients diffusion (release) and antioxidant activity of spent brewer yeast (SBY) was investigated. Three pre-treatments were performed: (1) autolysis at 50°C for 24 h; (2) autolysis at 50°C for 24 h, with the addition of β-mannanase during the autolysis; (3) autolysis at 50°C for 24 h, and the β-mannanase was added for another 12 h treatment. The pre-treatments with the addition of β-mannanase caused significant cell wall degradation; markedly increased the yield of SBY extracts...
June 21, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28636436/signal-on-fluorescent-sensor-based-on-n-cqds-for-the-detection-of-glutathione-in-human-serum-and-pharmaceutic-preparation
#11
Chengfei Zhao, Kangying Wang, Qihong Cai, Haijian Tu, Linghong Pan, Liumin Yu
Nitrogen-doped carbon quantum dots (N-CQDs) with citric acid and ethylenediamine as raw materials were synthesized by an efficient one-step strategy. The N-CQDs showed a special property that the fluorescence was quenched by Fe(3+). The quenched fluorescence of N-CQDs could be recovered by glutathione (GSH). Therefore, a "signal-on" fluorescent sensor was developed to detect GSH. The fluorescent sensor could favorably avoid the interference of ascorbic acid, dopamine, glucose, oxidized glutathione and other amino acids in the detecting process of GSH...
June 21, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28636431/enhanced-production-of-raw-starch-degrading-enzyme-using-agro-industrial-waste-mixtures-by-thermotolerant-rhizopus-microsporus-for-raw-cassava-chip-saccharification-in-ethanol-production
#12
Srisakul Trakarnpaiboon, Nantana Srisuk, Kuakoon Piyachomkwan, Kenji Sakai, Vichien Kitpreechavanich
In the present study, solid state fermentation for the production of raw starch degrading enzyme was investigated by thermotolerant Rhizopus microsporus TISTR 3531 using a combination of agro-industrial wastes as substrates. The obtained crude enzyme was applied for hydrolysis of raw cassava starch and chips at low temperature and subjected to non-sterile ethanol production using raw cassava chips. The agro-industrial wastes ratio was optimized using a simplex axial mixture design. The results showed that the substrate mixture consisting of rice bran: corncob: cassava bagasse at 8 g: 10 g: 2 g yielded the highest enzyme production of 201...
June 21, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28635559/purification-of-antibody-against-ara-h-2-by-a-home-made-immunoaffinity-chromatography-column
#13
Zhihua Wu, Kun Li, Shaode Zhan, Ping Tong, Xin Li, Anshu Yang, Hongbing Chen
Antibodies are used extensively in numerous applications both in vivo and in vitro. To purify anti-Ara h 2 polyclonal antibody (PcaAb), a home-made immunoaffinity chromatography column (IAC) method was established. The properties of the home-made column were compared with those of the Mab Affinity Protein G Agarose High Flow (MPG), a commercially available column successfully used in capturing polyclonal antibodies. During antibody purification from rabbits antiserum against Ara h 2, the column capacity, recovery, and purification factor were characterized for IAC and MPG...
June 21, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28635367/in-vivo-and-in-vitro-depolymerizations-of-intracellular-medium-chain-length-poly-3-hydroxyalkanoates-produced-by-pseudomonas-putida-bet001
#14
Siti Nor Syairah Anis, Mohamad Suffian Mohamad Annuar, Khanom Simarani
In vivo and in vitro depolymerizations of intracellular medium-chain-length poly-3-hydroxyalkanoates (mcl-PHA) in Pseudomonas putida Bet001 grown on lauric acid was studied. Both processes were studied under optimum conditions for mcl-PHA depolymerization viz. 0.2 M Tris-HCl buffer, pH 9, ionic strength (I) = 0.2 M at 30 °C. For in vitro depolymerization studies, cell-free system was obtained from lysing bacterial cells suspension by ultrasonication at optimum conditions (frequency 37 kHz, 30% of power output, <25 (°)C for 120 minutes)...
June 21, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/27136080/purification-and-partial-characterization-of-a-fibrinolytic-enzyme-from-the-fruiting-body-of-the-medicinal-and-edible-mushroom-pleurotus-ferulae
#15
Jun-Hui Choi, Dae-Won Kim, Seung Kim, Sung-Jun Kim
A fibrinolytic metalloprotease with in vitro fibrinolytic effects was purified from the edible mushroom Pleurotus ferulae using several chromatography steps including anion and ion exchange, gel filtration, and fast protein liquid chromatography columns. The molecular mass of the enzyme was estimated to be 20.0 kDa, as determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fibrin zymography. The protease was active at 50°C, and pH 4.0, 5.0, and 8.0. The fibrinolytic activity of the enzyme was inhibited by ethyleneglycol-bis-(2-aminoethyl)-N,N,N',N' tetraacetic acid and strongly inhibited by two metal ions, Cu and Mg...
July 3, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28267413/high-production-of-xanthan-gum-by-a-glycerol-tolerant-strain-xanthomonas-campestris-wxlb-006
#16
Zichao Wang, Jianrong Wu, Min-Jie Gao, Li Zhu, Xiao-Bei Zhan
The superior properties of xanthan gum make it an industrial aginomoto used in many industries, especially in oil recovery. In the present work, xanthan production from glycerol by a mutant strain Xanthomonas campestris WXLB-006 reached as high as 17.8 g/L in flask culture. With the adoption of pH control, varied aeration and agitation, and varied glycerol feeding strategy, xanthan production reached 33.9 g/L in a 7-L fermenter and fermentation time decreased to 60 hr. Instead of difficultly and costly purifying glycerol, this research provides a very good case for glycerol utilization...
May 28, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28448745/medium-optimization-for-pyrroloquinoline-quinone-pqq-production-by-methylobacillus-sp-zju323-using-response-surface-methodology-and-artificial-neural-network-genetic-algorithm
#17
Peilian Wei, Zhenjun Si, Yao Lu, Qingfei Yu, Lei Huang, Zhinan Xu
Methylobacillus sp. zju323 was adopted to improve the biosynthesis of pyrroloquinoline quinone (PQQ) by systematic optimization of the fermentation medium. The Plackett-Burman design was implemented to screen for the key medium components for the PQQ production. CoCl2 · 6H2O, ρ-amino benzoic acid, and MgSO4 · 7H2O were found capable of enhancing the PQQ production most significantly. A five-level three-factor central composite design was used to investigate the direct and interactive effects of these variables...
April 27, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28430025/synthesis-of-dopamine-in-e-coli-using-plasmid-based-expression-system-and-its-marked-effect-on-host-growth-profiles
#18
Arunangshu Das, Anita Verma, Krishna J Mukherjee
L-Dopa and dopamine are important pathway intermediates toward the synthesis of catecholamine such as epinephrine and norepinephrine from amino acid L-tyrosine. Dopamine, secreted from dopaminergic nerve cells, serves as an important neurotransmitter. We report the synthesis of dopamine by extending the aromatic amino acid pathway of Escherichia coli DH5α by the expression of 4-hydroxyphenylacetate-3-hydrolase (HpaBC) from E. coli and an engineered dopa decarboxylase (DDC) from pig kidney cell. The activity of HpaBC and DDC require 200 µM iron supplementation and 50 µM vitamin B6, respectively as additives to the growth media...
April 21, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/27824292/production-of-nanocellulose-in-miniature-bioreactor-optimization-and-characterization
#19
Sepideh Khazeni, Ashrafalsadat Hatamian-Zarmi, Fatemeh Yazdian, Zahra Beagom Mokhtari-Hosseini, Bahman Ebrahimi-Hosseinzadeh, Behnam Noorani, Ghassem Amoabedini, Mohammad Reza Soudi
Bacterial cellulose (BC) is a very fascinating microbial biopolymer which is mainly produced by Gluconacetobacter xylinum. Optimization of BC production by G. xylinum was performed based on scale-down studies in miniature-bioreactor and response surface methodology in which the optimum pH value (6.5) and shaking rate (50 rpm) were obtained. The static culture condition for BC production has newly been defined. Nanostructure of BC includes nanofibers up to (60 nm) and nanoporosity up to (265 nm) was observed by scanning electron microscopy...
April 21, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/27824279/agricultural-wastes-as-substrates-for-%C3%AE-glucosidase-production-by-talaromyces-thermophilus-role-of-these-enzymes-in-enhancing-waste-paper-saccharification
#20
Hanen Mallek-Fakhfakh, Jawhar Fakhfakh, Najla Masmoudi, Fatma Rezgui, Ali Gargouri, Hafedh Belghith
In the present study, we investigated a potent extracellular β-glucosidases secreted by the thermophilic fungal strain AX4 of Talaromyces thermophilus, isolated from Tunisian soil samples. This strain was selected referring to the highest thermostability of its β-glucosidases compared to the other fungal isolates. The β-glucosidase production was investigated by submerged fermentation. The optimal temperature and initial pH for maximum β-glucosidase production were 50°C and 7.0, respectively. Several carbon sources were assayed for their effects on β-glucosidase production, significant yields were obtained in media containing lactose 1% (3...
April 21, 2017: Preparative Biochemistry & Biotechnology
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