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Preparative Biochemistry & Biotechnology

Johan S Hero, José H Pisa, Nora I Perotti, Cintia M Romero, María A Martínez
The behaviour of three isolates retrieved from different cellulolytic consortia, Bacillus sp. AR03, Paenibacillus sp. AR247 and Achromobacter sp. AR476-2, were examined individually and as co-cultures in order evaluate their ability to produce extracellular cellulases and xylanases. Utilizing a peptone-based medium supplemented with carboxymethyl cellulose (CMC), an increase estimated of 1.30 and 1.50 times was obtained by the co-culture containing the strains AR03 and AR247, with respect to enzyme titles registered by their individual cultivation...
January 20, 2017: Preparative Biochemistry & Biotechnology
J L Navarrete-Bolaños, G P Téllez-Martínez, R Miranda-López, H Jiménez-Islas
For any fermentation process, the production cost depends on several factors, such as the genetics of the microorganism, the process condition and the culture medium composition. In this work, a guideline for the design of cost-efficient culture media using a sequential approach based on response surface methodology is described. The procedure was applied to analyze and optimize a culture medium of registered trademark and a base culture medium obtained as a results of the screening analysis from different culture media used to grow the same strain according to the literature...
January 19, 2017: Preparative Biochemistry & Biotechnology
Dongsheng Xu, Aini Wan, Lin Peng, Yun Chen, Yang He, Jianfeng Yang, Jian Jin
Hepatocyte growth factor (HGF) is a potent multi-functional cytokine that affects proliferation, migration and morphogenesis of various cells. HGF is secreted as an inactive single chain precursor protein and activated by the cleavage of serine proteases to form heterodimers. In our current study, the cleavage site of HGF was blocked by replaced Arg 494 of Glu (R494E) that resulted in the single chain HGF (R494E) unable to be cleaved by serine proteases. We established Chinese hamster ovary (CHO) cells overexpressing HGF (R494E), the expression of HGF (R494E) achieved 12 mg/L and was similar to a previously reported study...
January 3, 2017: Preparative Biochemistry & Biotechnology
Sachinkumar Birhade, Mukesh Pednekar, Shilpa Sagwal, Annamma Odaneth, Arvind Lali
Controlled depolymerization of cellulose is essential for the production of valuable cellooligosaccharides and cellobiose from lignocellulosic biomass. However, enzymatic cellulose hydrolysis involves multiple synergistically acting enzymes, making difficult to control the depolymerization process and generate desired product. This work exploits the varying adsorption properties of the cellulase components to the cellulosic substrate and aims to control the enzyme activity. Cellulase adsorption was favored on pretreated cellulosic biomass as compared to synthetic cellulose...
January 3, 2017: Preparative Biochemistry & Biotechnology
Alireza Moosavi Zenooz, Farzin Zokaee Ashtiani, Reza Ranjbar, Fatemeh Nikbakht, Oberon Bolouri
Biodiesel production from microalgae feedstock should be performed after growth and harvesting of the cells and the most feasible method for harvesting and dewatering of microalgae is flocculation. Flocculation modeling can be used for evaluation and prediction of its performance under different affective parameters. However, the modeling of flocculation in microalgae is not simple and has not performed yet, under all experimental conditions, mostly due to different behaviors of microalgae cells during the process under different flocculation conditions...
January 3, 2017: Preparative Biochemistry & Biotechnology
Alenir Naves de Sales, Angélica Cristina de Souza, Rondinele de Oliveira Moutta, Viridiana Santana Ferreira-Leitão, Rosane Freitas Schwan, Disney Ribeiro Dias
Actinobacteria isolates from Brazilian Cerrado soil were evaluated for their ability to produce enzymes of the cellulolytic and xylanolytic complex using lignocellulose residual biomass. Preliminary semi-quantitative tests, made in Petri plates containing carboxymethylcellulose (CMC) and beechwood xylan, indicated 11 potential species producing enzymes, all belonging to the genus Streptomyces. The species were subsequently grown in pure substrates in submerged fermentation and analyzed for the production of enzymes endoglucanase, β-glucosidase, endoxylanase and β-xylosidase...
January 3, 2017: Preparative Biochemistry & Biotechnology
V Guru KrishnaKumar, Sharad Gupta
Tau is an intrinsically disordered protein responsible for maintaining the structure and stability of axonal microtubules. However, in certain disease conditions including Alzheimer's disease, tau protein may undergo biochemical and structural changes to form intracellular aggregates. Since tau is a proline and arginine rich eukaryotic protein, heterologous expression in E. coli often results in poor yield and has been a major technical challenge. In the current work, we have improved the expressed yield of tau by overcoming codon bias problem and established a simplified protocol for efficient extraction...
January 3, 2017: Preparative Biochemistry & Biotechnology
Juliet Victoria, Annamma Odaneth, Arvind Lali
Depolymerisation of lignocellulosic biomass is catalysed by groups of enzymes whose action is influenced by substrate features and the composition of cellulase preparation. Cellulases contain a mixture of variety of enzymes, whose proportions dictate the saccharification of biomass. In the current study, four cellulase preparations varying in their composition were used to hydrolyse two types of alkali treated biomass (aqueous ammonia treated rice straw and sodium hydroxide treated rice straw) to study the effect on catalytic rate, saccharification yields and sugar release profile...
January 3, 2017: Preparative Biochemistry & Biotechnology
Ali Saber Abdelhameed, Saima Nusrat, Sanjhi Paliwal, Masihuz Zaman, Nida Zaidi, Rizwan Hasan Khan
Drug and protein interaction provides a structural guideline in the rational drug designing and in the synthesis of new and improved drugs with greater efficacy. We have examined here the binding affinity and mechanism of nintedanib (NTB), an anticancer drug (Tyrosine kinase inhibitor) with bovine serum albumin (BSA), under physiological conditions, by spectroscopic techniques. The decrease in Stern-Volmer quenching constants and binding constant with increase in temperature suggests BSA forms a complex with NTB...
January 3, 2017: Preparative Biochemistry & Biotechnology
Young-Ran Song, Sang-Ho Baik
A gene encoding cinnamoyl esterase (CE), which breaks down chlorogenic acid (ChA) into caffeic and quinic acids, was cloned from Lactobacillus helveticus KCCM 11223. The gene with an open reading frame (ORF) of 759 nucleotides was expressed in Escherichia coli, which resulted in a 51.6-fold increase in specific activity compared to L. helveticus KCCM 11223. The recombinant CE exists as a monomeric enzyme having a molecular weight of 27.4 kDa. Although the highest activity was observed at pH 7, the enzyme showed stable activity at pH 4...
January 3, 2017: Preparative Biochemistry & Biotechnology
Chunqiao Xiao, Xiaoyan Wu, Tingting Liu, Guang Xu, Ruan Chi
Microbial solubilization of rock phosphate is getting more and more attention recently. However, the microorganisms used in previous studies were mostly single or known species, and seldom studies focused on the mixed microorganisms or microbial consortia from natural environments. In this study, a microbial consortium taken from activated sludge was used to solubilize two different mid-low grade rock phosphates. Results showed that the microbial consortium could effectively solubilize the rock phosphates in National Botanical Research Institute's phosphate growth medium and released soluble phosphorus in the broth...
December 29, 2016: Preparative Biochemistry & Biotechnology
Sócrates Palacios-Ponce, Rodolfo Ramos-González, Héctor A Ruiz, Miguel A Aguilar, José L Martínez-Hernández, Elda P Segura-Ceniceros, Cristóbal N Aguilar, Georgina Michelena, Anna Ilyina
In the present study, the interactions between chitosan-coated magnetic nanoparticles (C-MNP) and Trichoderma sp. spores as well as Kluyveromyces marxianus cells were studied. By means of Plackett-Burman design, it was demonstrated that factors which directly influenced on yeast cells immobilization and magnetic separation were: inoculum and C-MNP quantity, stirring speed, interaction time, and volume of medium, while in the case of fungal spores, the temperature also was disclosed as an influencing factor...
December 29, 2016: Preparative Biochemistry & Biotechnology
Dario Rafael Olicón-Hernández, Pedro Alberto Vázquez-Landaverde, Ramón Cruz-Camarillo, Luz Irene Rojas-Avelizapa
Bacillus thuringiensis is a non-human pathogen bacterium that is used as a fungal and insect biocontrol agent. Because of its environmental interaction, it possesses several extracellular enzymes that are able to degrade chitin and chitosan, two of the most important polymers because of their application in numerous fields. However, in recent years, it has been observed that oligosaccharides from the enzymatic degradation of chitosan have important benefits for human health. Compare and explore the production of chitooligosaccharides from different sources of chitosan will improve the process parameters and expand the biotechnology based in this molecules...
November 10, 2016: Preparative Biochemistry & Biotechnology
Sepideh Khazeni, Ashrafalsadat Hatamian-Zarmi, Fatemeh Yazdian, Zahra Beagom Mokhtari-Hosseini, Bahman Ebrahimi-Hosseinzadeh, Behnam Noorani, Ghassem Amoabedini, Mohammad Reza Soudi
Bacterial cellulose (BC) is a very fascinating microbial biopolymer which is mainly produced by Gluconacetobacter xylinum. Optimization of BC production by G. xylinum was performed based on scale-down studies in miniature-bioreactor and response surface methodology in which the optimum pH value (6.5) and shaking rate (50 rpm) were obtained. The static culture condition for BC production has newly been defined. Nanostructure of BC includes nanofibers up to (60 nm) and nanoporosity up to (265 nm) was observed by scanning electron microscopy...
November 8, 2016: Preparative Biochemistry & Biotechnology
Hanen Mallek-Fakhfakh, Jawhar Fakhfakh, Najla Masmoudi, Fatma Rezgui, Ali Gargouri, Hafedh Belghith
In the present study, we investigated a potent extracellular β-glucosidases secreted by the thermophilic fungal strain AX4 of Talaromyces thermophilus, isolated from Tunisian soil samples. This strain was selected referring to the highest thermostability of its β-glucosidases compared to the other fungal isolates. The β-glucosidase production was investigated by submerged fermentation. The optimal temperature and initial pH for maximum β-glucosidase production were 50°C and 7.0, respectively. Several carbon sources were assayed for their effects on β-glucosidase production, significant yields were obtained in media containing lactose 1% (3...
November 8, 2016: Preparative Biochemistry & Biotechnology
Siriporn Chaikaew, Apinun Kanpiengjai, Jenjira Intatep, Kridsada Unban, Pairote Wongputtisin, Goro Takata, Chartchai Khanongnuch
The present study demonstrates the effectiveness of X-ray radiation in strain improvement for defective lipase production by Bacillus sp. MR10 for further application in the fermentative production of manno-oligosaccharides (MOS) from agricultural by-product, defatted copra meal (DCM). The mutants obtained were screened based on their defective lipase activity together with their β-mannanase production performance. Among 10 selected mutants, the strain M7 was the highest promising mutant regarding the smallest lipase activity (0...
November 7, 2016: Preparative Biochemistry & Biotechnology
Ashish A Prabhu, A Jayadeep
The current study is focused on optimizing the parameters involved in enzymatic processing of red rice bran for maximizing total polyphenol (TP) and free radical scavenging activity (FRSA). The sequential optimization strategies using central composite design (CCD) and artificial neural network (ANN) modeling linked with genetic algorithm (GA) was performed to study the effect of incubation time (60-90 min), xylanase concentration (5-10 mg/g), cellulase concentration (5-10 mg/g) on the response, i.e., total polyphenol and FRSA...
November 4, 2016: Preparative Biochemistry & Biotechnology
Iszatty Ismail, Tana Poorani Gurusamy, Hema Ramachandran, Abdullah Al-Ashraf Amirul
Antibiofilm polymers have the ability to inhibit bacterial biofilm formation, which is known to occur ubiquitously in the environment and pose risks of infection. In this study, production of P(3HB-co-4HB) copolymer and antimicrobial yellow pigment from Cupriavidus sp. USMAHM13 are enhanced through medium optimization. Before the improvement of yellow pigment production, screening for the best additional supplement was performed resulting in high-yield yellow pigmentation using yeast extract with optimum concentration of 2 g/L...
November 4, 2016: Preparative Biochemistry & Biotechnology
Moolchand Sigar, Nitu Maity, Saroj Mishra
Protein fusion technology has emerged as one of the important strategies to increase the level of expression and half-life of therapeutic proteins in heterologous expression systems. Granulocyte colony-stimulating factor (G-CSF) is a hematopoietic growth factor and is clinically used against neutropenia. Enhanced expression and stability of G-CSF were achieved in Pichia pastoris by the way of constructing a fusion protein with human serum albumin (HSA). The strategy involved polymerase chain reaction (PCR) amplification of fragments corresponding to codon-optimized G-CSF and domain 3 of HSA...
November 4, 2016: Preparative Biochemistry & Biotechnology
Habibollah Faraji, Mohammad Ramezani, Hamid Reza Sadeghnia, Khalil Abnous, Fatemeh Soltani, Baratali Mashkani
Staphylokinase (SAK) as the third generation thrombolytic molecule is a promising agent for the treatment of thrombosis. SAK variant of SAKфC was expressed in Pichia pastoris strains KM71H and GS115. The codon adaptation index of SAK was improved from 0.75 to 0.89. The expression of recombinant SAK (rSAK) reached to its maximum (310 mg/L of the culture medium) after 48-hr stimulation with 3% methanol and remained steady until day 5. The maximum activity of the enzyme was at pH 8.6 and 37°C. It was highly active at temperatures 20-37°C and pH ranges of 6...
November 4, 2016: Preparative Biochemistry & Biotechnology
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