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Preparative Biochemistry & Biotechnology

V Gohel, K Ranganathan, G Duan
Conventional grain ethanol manufacturing is a high temperature energy intensive process comprising of multiple unit operations when combined with lower ethanol recovery results in higher production cost. In liquefaction, jet cooking accounts for significant energy cost while strong acid or base used for pH adjustment presents a safety hazard. A need is felt for sustainable ethanol manufacturing process that is less hazardous, consumes lower energy and operates in a low pH range of 4.50 to 5.50. A Single Temperature Liquefaction (STL) process that could efficiently operate at lower liquefaction temperature over a pH range of 4...
October 13, 2016: Preparative Biochemistry & Biotechnology
Eun Jae Kim, Jun Hyuck Lee, Sung Gu Lee, Se Jong Han
To survive in a sub-zero environment, polar organisms produce ice-binding proteins (IBPs). These IBPs prevent the formation of large intracellular ice crystals, which may be fatal to the organism. Recently, a recombinant FfIBP (an IBP from Flavobacterium frigoris PS1) was cloned and produced in Pichia pastoris using fed-batch fermentation with methanol feeding. In this study, we demonstrate that FfIBP produced by P. pastoris has a glycosylation site, which diminishes the thermal hysteresis (TH) activity of FfIBP...
October 13, 2016: Preparative Biochemistry & Biotechnology
K Divakar, M Suryia Prabha, G Nandhinidevi, Pennathur Gautam
The simultaneous production of intracellular esterase and extracellular protease from the strain Lysinibacillus fusiformis AU01 was studied in detail. The production was carried out both under batch and fedbatch mode. The maximum yield of intracellular esterase and protease was obtained under full oxygen saturation at the beginning of the fermentation. The data was fitted to the Luedeking-Piret model and it was shown that the enzyme (both esterase and protease) production was growth-associated. A decrease in intracellular esterase and increase in the extracellular esterase was observed during late stationary phase...
October 13, 2016: Preparative Biochemistry & Biotechnology
Yan-Yang Xu, Yang Qiu, Hui Ren, Dong-Hu Ju, Hong-Lei Jia
Aronia melanocarpa berries are abundant in polyphenolic compounds. After juice production, the pomace of pressed berries still contains a substantial amount of polyphenolic compounds. For efficient utilization of Aronia melanocarpa berries and the enhancement of polyphenolic compounds yields in Aronia melanocarpa pomace (AMP), total phenolics (TP) and total flavonoids (TF) from AMP were extracted, using ultrasound-assisted aqueous two-phase system (UAE-ATPS) extraction method. Firstly, the influences of ammonium sulfate concentration, ethanol-water ratio, ultrasonic time and ultrasonic power on TP and TF yields were investigated...
October 13, 2016: Preparative Biochemistry & Biotechnology
Paran Gani, Norshuhaila Mohamed Sunar, Hazel Matias-Peralta, Ab Aziz Abdul Latiff, Siti Fatimah Zaharah Mohamad Fuzi
Microalgae biomass has been recognised as a sustainable bio-product to replace terrestrial biomass in biofuel production. The microalgae industry has high operating costs, specifically on harvesting and biomass recovery. Therefore, the development of an efficient harvesting method is crucial to the minimisation of production cost. A statistical analysis via response surface methodology was used to investigate the optimisation of harvesting efficiency using alum and chitosan as a coagulant. Growth rate and biomass productivity were also determined...
October 13, 2016: Preparative Biochemistry & Biotechnology
Aleksandra Margetić, Zoran Vujčić
Yeast Saccharomyces cerevisiae is the most significant source of enzyme invertase. It is mainly used in the food industry as a soluble or immobilized enzyme. The greatest amount of invertase is located in the periplasmic space in yeast. In this work it was isolated two forms of enzyme from yeastS. cerevisiae cell, soluble and cell wall invertase (CWI). Both forms of enzyme showed same temperature optimum (60°C), similar pH optimum and kinetic parameters. The significant difference between these biocatalysts was observed in their thermal stability, stability in urea and methanol solution...
October 13, 2016: Preparative Biochemistry & Biotechnology
Ahmed Abd Elrazak, Alan C Ward, Jarka Glassey
The increase demand of Omega-3 in the market and the challenges facing its conventional supplies led to an increase interest to microbial Omega-3 sources. This research concentrates on the statistical role of some metal ions on the biosynthesis and productivity of eicosapentanoic acid (essential omega-3 element) in bacterial isolate, Shewanella 717. A Plackett-Burman design was applied to screen the main effect of all metal salts entrenched in the Artifical Sea Water medium components. Four salts in particular in addition to the interaction among them were highlighted as having a statistically significant effect upon the growth and/or EPA production...
October 13, 2016: Preparative Biochemistry & Biotechnology
Sumitra Datta, Gopalakrishnan Menon, Bincy Varughese
Proteolytic Aeromonas caviae P-1-1 growing at wide ranging pH (7.0-11.0) and moderate salinity (0-5% NaCl) was isolated from cattle-shed of Thanjavur, India. It produced lipase, gelatinase and polyhydroxybutyrate. Different culture conditions, incubation time, carbon and nitrogen sources, vitamins, amino acids, surfactants and metal ions for optimal growth and protease production of P-1-1 were examined. Maximum protease (0.128 U/mL) production was achieved with 1% fructose, 1% yeast extract, 0.1% ammonium sulfate, 3% NaCl, 0...
October 13, 2016: Preparative Biochemistry & Biotechnology
Karla Silva Teixeira Souza, Cintia Lacerda Ramos, Rosane Freitas Schwan, Disney Ribeiro Dias
The main carbon source used for growth by four yeast strains (Yarrowia lipolytica CCMA 0357, Y. lipolytica CCMA 0242, Wickerhamomyces anomalus CCMA 0358, and Cryptococcus humicola CCMA 0346) and their lipid production were evaluated, using different concentrations of crude and pure glycerol and glucose. Whereas crude glycerol (100 g/L) was the main carbon source used by Y. lipolytica CCMA 0357 (nearly 15 g/L consumed at 120 h) and W. anomalus CCMA 0358 (nearly 45.10 g/L consumed at 48 h), pure glycerol (150 g/L) was the main one used by C...
October 13, 2016: Preparative Biochemistry & Biotechnology
Chunyan Chen, Sihua Long, Airong Li, Guoqing Xiao, Linyuan Wang, Zeyi Xiao
Since both ethanol and butanol fermentations are urgently developed processes with the biofuel demand increasing, performance comparison of aerobic ethanol fermentation and anerobic butanol fermentation in a continuous and closed-circulating fermentation (CCCF) system was necessary to achieve their fermentation characteristics and further optimize the fermentation process. Fermentation and pervaporation parameters including the average cell concentration, glucose consumption rate, cumulated production concentration, product flux and separation factor of ethanol fermentation were 11...
August 24, 2016: Preparative Biochemistry & Biotechnology
Érika Lika Ida, Ronivaldo Rodrigues da Silva, Tássio Brito de Oliveira, Tatiane Beltramini Souto, Juliana Abigail Leite, André Rodrigues, Hamilton Cabral
Filamentous fungi secrete diverse peptidases with different biochemical properties, which is of considerable importance for application in various commercial sectors. In this study, we describe the isolation of two fungal species collected from the soil of decayed organic matter: Aspergillus fischeri and Penicillium citrinum. In a submerged bioprocess, we observed better peptidase production with the fungus P. citrinum, which reached a peak production at 168 h with 760 U/mL, in comparison to that with the fungus A...
August 23, 2016: Preparative Biochemistry & Biotechnology
Prawphan Yuvadetkun, Noppol Leksawasdi, Mallika Boonmee
Candida shehatae ATCC 22984, a xylose-fermenting yeast, showed an ability to produce ethanol in both glucose and xylose medium. Maximum ethanol produced by the yeast was 48.8 g/L in xylose and 52.6 g/L in glucose medium with ethanol yields varied between 0.3-0.4 g/g depended on initial sugar concentrations. Xylitol was a co-product of ethanol production using xylose as substrate and glycerol was detected in both glucose and xylose media. Kinetic model equations indicated that growth, substrate consumption and products formation of C...
August 23, 2016: Preparative Biochemistry & Biotechnology
Gaoxiang Qi, Fen Peng, Lian Xiong, Xiaoqing Lin, Chao Huang, Hailong Li, Xuefang Chen, Xinde Chen
Extraction of high-value products from agricultural wastes is an important component for sustainable bio-economy development. In this study, wax extraction from sugarcane bagasse was carried out and the beneficial effect of dewaxing pretreatment on the enzymatic hydrolysis was investigated. 1.2% (w/w) of crude sugarcane wax was obtained from the sugarcane bagasse using the mixture of petroleum ether and ethanol (mass ratio of 1:1) as the extraction agent. Results of FT-IR characterization and GC-MS qualitative analysis showed that the crude sugarcane wax consisted of fatty fractions (fatty acids, fatty aldehydes, hydrocarbons and esters) and small amount of lignin derivatives...
August 18, 2016: Preparative Biochemistry & Biotechnology
Sirma Yegin
Statistical optimization of the factors affecting xylanase production by Aureobasidium pullulans NRRL Y-2311-1 on globe artichoke stem was performed for the first time. The optimization strategies used resulted in almost 6-fold enhancement of xylanase production (66.48 U/ml). Biochemical and thermal characterization of the crude xylanase preparation was performed in order to elucidate its feasibility for different industrial applications. The optimum conditions for xylanase activity were: pH 4.0 and 30-50°C...
August 18, 2016: Preparative Biochemistry & Biotechnology
Ningjun Duan, Wansheng Lv, Lingli Zhu, Weijuan Zheng, Zichun Hua
Hyaluronan is a widely distributed glycosaminoglycan which has multiple functions. HA accumulation has been reported in many human diseases. Understanding the role of hyaluronan and its binding proteins in the pathobiology of disease will facilitate the development of novel therapeutics for many critical diseases. Current techniques described for the analysis of HA are mainly for HA quantification in solutions, not for the direct detection of HA in tissues or on cell surfaces. In our study, a fusion protein, named RHC-EGFP, combined the HA binding domain, C-terminal of Receptor for Hyaluronan-mediated Motility (RHAMM), with EGFP, a widely used enhanced green fluorescence protein, was expressed and purified from E...
August 18, 2016: Preparative Biochemistry & Biotechnology
Saravanan R, Shajahan Shubethar, Narayanan S, Manish Jain, Shankar Lade, Deepak Jadhav, Maheswaran P, Uday K Avalakki, Ashok Kumar Dubey
Galactooligosaccharides (GOS) are non-digestible dietary fibers which have a beneficial effect on human health by promoting the growth of probiotic bacteria in the gut. In addition, other health benefits have been reported from oligosaccharides consumption such as stimulation of intestinal mobility, colon cancer prevention, mineral absorption as well as protection against certain pathogenic bacterial infections. The goal of this research was to develop an efficient biotransformation system using a consortium of microbes for the production of ≥ 85% pure GOS and reusing the cell biomass in repeated cycles of biotransformation...
August 15, 2016: Preparative Biochemistry & Biotechnology
Xuewei Pang, Yitao Zheng, Xianting Qiao, Quangui Mao, Qian Ma, Ruifang Ye
Lincomycin is a lincosamide antibiotic produced by Streptomyces lincolnensis. Through mutagenesis by ethylmethansulfonate (EMS) and ultraviolet (UV) irradiation repeatedly, M2 was picked out in plate with glutamine and propylproline orderly. In 50-L stirred bioreactor, the production of lincomycin, fermented by M2, was increased to 8136 u/ml under the optimal condition as compared to original strainS. lincolnensis 07-5 (6634 u/ml). Two-dimensional gel electrophoresis (2-D GE) and mass spectrometry (MS) shown LmbG, LmbI and acetohydroxy acid isomeroreductase were remarkably synthesized in M2...
August 15, 2016: Preparative Biochemistry & Biotechnology
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No abstract text is available yet for this article.
November 16, 2016: Preparative Biochemistry & Biotechnology
Kunal Kumar, Prasanna D Belur
Oxalate oxidase (EC catalyzes the oxidative cleavage of oxalate to carbon dioxide with the reduction of molecular oxygen to hydrogen peroxide. Oxalate oxidase found its application in clinical assay for oxalate in blood and urine. This study describes the purification and biochemical characterization of an oxalate oxidase produced from an endophytic bacterium, Ochrobactrum intermedium CL6. The cell-free fermentation broth was subjected to two-step enzyme purification, which resulted in a 58.74-fold purification with 83% recovery...
October 2, 2016: Preparative Biochemistry & Biotechnology
Thunyaporn Viriyayingsiri, Pantaporn Sittplangkoon, Sorawit Powtongsook, Kasidit Nootong
Continuous production of diatom Entomonies sp. was performed in mechanically stirred tank and flat-panel airlift photobioreactors (FPAP). The maximum specific growth rate of diatom from the batch experiment was 0.98 d(-1). A series of dilution rate and macronutrient concentration adjustments were performed in a stirred tank photobioreactor and found that the dilution rate ranged from 0.7 to 0.8 d(-1) and modified F/2 growth media containing nitrate at 3.09 mg N/L, phosphate at 2.24 mg P/L, and silicate at 11...
October 2, 2016: Preparative Biochemistry & Biotechnology
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