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Preparative Biochemistry & Biotechnology

Lijuan Ma, Qing Ma, Gaojie Guo, Liping Du, Yingying Zhang, Youzhi Cui, Dongguang Xiao
Sodium percarbonate (SP), a kind of alkaline strong oxidant, was applied to corncob pretreatment. The optimized pretreatment conditions were at 4% (w/v) SP concentration with solid-to-liquid (SLR) ratio of 1:10 treating for 4 hr at 60°C. This pretreatment resulted in 91.06% of cellulose and 84.08% of hemicellulose recoveries with 34.09% of lignin removal in corncob. The reducing sugar yield from SP-pretreated corncob was 0.56 g/g after 72 hr of enzymatic hydrolysis, 1.75-folds higher than that from raw corncob...
March 12, 2018: Preparative Biochemistry & Biotechnology
Thanaporn Wannachod, Sutthinee Wannasutthiwat, Sorawit Powtongsook, Kasidit Nootong
Photoautotrophic cultivation of Chlorococcum humicola was performed in batch and continuous modes in different cultivating system arrangements to compare biomass and carotenoids concentration and their productivities. Batch result from stirred tank and airlift photobioreactors indicated the positive effect of increasing light intensity on growth and carotenoids production, whereas the finding from continuous cultivation indicated that carotenoids enhancement preferred high light intensity and nitrogen deficient environment...
March 7, 2018: Preparative Biochemistry & Biotechnology
Shruthi Sunderrajan, Lima Rose Miranda, Gautam Pennathur
Graphene oxide/ chitosan and reduced graphene oxide/ chitosan (GO/CS and RGO/CS) beads were prepared by precipitation with NaOH. Porcine liver esterase was immobilized on these beads to give GO/CS/E and RGO/CS/E beads. The optimum conditions for the maximum activity of RGO/CS/E beads was pH 8 and 50°C. The stability of the enzyme immobilized on GO/CS/E and RGO/CS/E was high in the pH range of 5-8. The GO/CS/E beads showed superior stability compared to that of the free enzyme and CS/E beads between 20°C and 50°C...
March 6, 2018: Preparative Biochemistry & Biotechnology
Mark-Henry Kamga, Maurizio Cattaneo, Seongkyu Yoon
A new integrated continuous biomanufacturing platform for continuous production of antibodies at fixed cell volumes and cell concentrations for extended periods with immediate capture is presented. Upstream antibody production has reached technological maturity, however, the bottleneck for continuous biomanufacturing remains the efficient and cost effective capture of therapeutic antibodies in an initial chromatography step. In this study, the first successful attempt at using one-column continuous chromatography (OCC) for the continuous capture of therapeutic antibodies produced through alternating tangential flow (ATF) perfusion is presented...
March 6, 2018: Preparative Biochemistry & Biotechnology
Davood Mazaheri, Seyed Abbas Shojaosadati, Seyed Morteza Zamir, Seyyed Mohammad Mousavi
In this work, mathematical modelling of ethanol production in solid-state fermentation (SSF) has been done based on the variation in the dry weight of solid medium. This method was previously used for mathematical modelling of enzyme production, however, the model should be modified to predict the production of a volatile compound like ethanol. The experimental results of bioethanol production from the mixture of carob pods and wheat bran by Zymomonas mobilis in SSF were used for the model validation. Exponential and logistic kinetic models were used for modelling the growth of microorganism...
March 6, 2018: Preparative Biochemistry & Biotechnology
Thanongsak Chaiyaso, Wimada Srisuwan, Charin Techapun, Masanori Watanabe, Shinji Takenaka
The new amylolytic oleaginous red yeast, Sporidiobolus pararoseus KX709872, produced both α-amylase (540 ± 0.09 mU/mL) and amyloglucosidase (23 ± 0.00 mU/mL) and showed good ability to directly convert rice residue from canteen waste to biomass and lipids. Effects of medium composition and cultivation conditions on growth and lipid accumulation for strain KX709872 were investigated under shaking-flask and up-scaling levels. At C:N ratio of 25:1, pH 5.45, 22.36°C, and 199.40 rpm for 7 days, volumetric production of biomass and lipids, lipid content, and lipid productivity reached 17...
March 6, 2018: Preparative Biochemistry & Biotechnology
Mingcong Shao, Lili Xiu, Haijiang Zhang, Jianying Huang, Xingwen Gong
Chitosan/cellulose-based beads (CCB) for the affinity purification of histidine-tagged proteins were prepared from chitosan/cellulose dissolved in ionic liquid as solvent, and their structures were characterized by fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA). The affinity purification was used to separate hexahistidine-tagged (his-tagged) enhanced green fluorescent protein (EGFP) from Escherichia coli (E. coli). The results showed that Zn2 + -CCB exhibited more specific adsorption capacity towards the target protein compared with Ni2 + -CCB and Cu2 + -CCB...
March 6, 2018: Preparative Biochemistry & Biotechnology
M Mervan Çakar, Juan Mangas-Sanchez, William R Birmingham, Nicholas J Turner, Barış Binay
Over the next decades, with the growing concern of rising atmospheric carbon dioxide (CO2 ) levels, the importance of investigating new approaches for its reduction becomes crutial. Reclamation of CO2 for conversion into biofuels represents an alternative and attractive production method that has been studied in recent years, now with enzymatic methods gaining more attention. Formate dehydrogenases (FDHs) are NAD(P)H-dependent oxidoreductases that catalyse the conversion of formate into CO2 and have been extensively used for cofactor recycling in chemoenzymatic processes...
March 5, 2018: Preparative Biochemistry & Biotechnology
Rajat Pandey, Venkata Dasu Veeranki
Biosimilar/Biotherapeutic production is becoming a major area of focus for a big chunk of Biotechnology Industry. Easy licensing and already approved status for clinical use has given it a boost. In the present study, recombinant human interferon gamma was expressed for the first time in Kluyveromyces Lactis (K. lactis) expression system and its expression was optimized by varying growth parameters and carbon source concentration with the aim of increasing recombinant protein production level. Human Interferon gamma gene (IFNG) was cloned in the genomic DNA of K...
February 9, 2018: Preparative Biochemistry & Biotechnology
Aungkana Srisuwun, Nidtaya Tantiwa, Ampin Kuntiya, Arthitaya Kawee-Ai, Apisit Manassa, Charin Techapun, Phisit Seesuriyachan
This experiment aimed to decolorize Reactive Red 159 using a high potential of a consortium of Purple Non-Sulfur Bacteria (PNSB) with an application of Response Surface Methodology (RSM) via a Central Composite Design (CCD) in open system. The three factors of Hydraulic Retention Time (HRT), Sludge Retention Time (SRT) and dye concentration were applied to the design. The decolorization was operated in an Anaerobic Sequencing Batch Reactor (AnSBR) until the system reached to a pseudo-steady state for 30 cycles in each experiment...
February 9, 2018: Preparative Biochemistry & Biotechnology
Rajat Pandey, Nitin Kumar, Ashish A Prabhu, Venkata Dasu Veeranki
The present study is focused upon improving biomass of Kluyveromyces lactis (K. lactis) cells expressing recombinant human interferon gamma (hIFN-γ), with the aim of augmenting hIFN-γ concentration by using statistical and artificial intelligence approach. Optimization of medium components viz., lactose, yeast extract and trace elements was carried out with Box-behnken design (BBD) & artificial neural network linked genetic algorithm (ANN-GA) for maximizing biomass of recombinant K. lactis (objective function)...
February 9, 2018: Preparative Biochemistry & Biotechnology
Rajesh Kumar Sahoo, Mohit Kumar, Swati Mohanty, Matthew Sawyer, Pattanathu K S M Rahman, Lala Behari Sukla, Enketeswara Subudhi
The production of biofuel using thermostable bacterial lipase from hot spring bacteria out of low cost agricultural residue olive oil cake is reported in the present paper. Using a lipase enzyme from Bacillus licheniformis, a 66.5% yield of methyl esters were obtained. Optimum parameters were determined, with maximum production of lipase at a pH of 8.2, temperature 50.8°C, moisture content of 55.7% and bio-surfactant content of 1.693 mg. The contour plots and 3D surface responses depict the significant interaction of pH and moisture content with bio-surfactant during lipase production...
February 9, 2018: Preparative Biochemistry & Biotechnology
Shivani Chaturvedi, Arti Kumari, Lata Nain, Sunil K Khare
Production of lipid from oleaginous yeast using starch as carbon source is not a common practice, therefore the purpose of this investigation was to explore the capability of starch assimilating microbes to produce oil, which was determined in terms of biomass weight, productivity & lipid yield. Saccharomyces pastorianus, Rhodotorula mucilaginosa, R. glutinis, and fungal isolate Ganoderma wiiroense were screened for the key parameters. The optimization was also carried out by one factor at a time approach. Considering the specific yield of lipid and cell dry weight yield, R...
February 9, 2018: Preparative Biochemistry & Biotechnology
Sandeep Shewale, Virendra K Rathod
The objective of this work is to put forth the optimization and kinetics of total phenolic compound extraction from A.indica leaves in a stirred batch extraction. Various experiential extraction parameters have been studied for maximum extraction of the total phenolic compounds. The maximum yield of total phenolic compounds was found to be 10.80 mg/g of dried neem powder at the optimized conditions. The extraction kinetics behavior followed first-order kinetics with diffusion coefficient ranged from 1.8 × 10-12 to 3...
February 9, 2018: Preparative Biochemistry & Biotechnology
Himani Garg, Jagdish C Gupta, G P Talwar, Shweta Dubey
Survivin has received attention as a potential target for cancer immunotherapy because of its crucial role in oncogenesis. We undertook this study to evaluate the immunotherapeutic potential of combination of recombinant Survivin along with adjuvant alum and immune modulator Mycobacterium indicus pranii (MIP). In vivo efficacy of the combination was studied in an invasive murine breast cancer model. Recombinant Survivin protein was purified from E. coli based expression system and characterized by western blotting...
January 22, 2018: Preparative Biochemistry & Biotechnology
Jianguang Liang, Jie Zhao, Zejian Wang, Yonghong Wang
Pyruvate oxidase (PyOD) is a very powerful enzyme for clinical diagnostic applications and environmental monitoring. Influences of temperature on cell growth, plasmid stability and PyOD expression during the PyOD fermentation process by recombinant E. coli were investigated. Based on the influences of temperature on the physiological metabolism, a novel high cell density fed-batch cultivation with gradient temperature decrease strategy for effective PyOD production was achieved, under which the biomass (OD600) of recombinant E...
January 22, 2018: Preparative Biochemistry & Biotechnology
Liangzhi Li, Pei Kang, Xin Ju, Jiajia Chen, Huibin Zou, Cuiying Hu, Lishi Yan
Erythritol, a well-known natural sweetener, is mainly produced by microbial fermentation. Various metal ions (Al3+, Cu2+, Mn2+, and Ni2+) were added to the culture medium of Trichosporonoides oedocephalis ATCC 16958 at 30 mg/L in shake flask cultures. Compared with controls, Cu2+ increased the erythritol content by 86% and decreased the glycerol byproduct by 31%. After 48 h of shake flask culture, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed that expression levels of erythrose reductase (ER) in presence of 30 mg/L CuSO4 · 5H2O were higher than those obtained after treatment with other examined metal ions...
January 22, 2018: Preparative Biochemistry & Biotechnology
Ketki Behere, Bumjoon Cha, Seongkyu Yoon
A modified shrinking core model (MSCM) has been employed to describe the mechanism for the degradation of Protein A resin particles taking place under continuous chromatographic operation. The model is based on the hypothetical shrinkage of the boundary layer of the resin particles, which house the active Protein A ligands within their pores. It has been determined that caustic during the sanitization phase of chromatography primarily caused the Protein A ligand degradation. Protein A resins provided by manufacturers possess unique caustic stability which has been employed in MSCM to appraise the ligand degradation...
January 22, 2018: Preparative Biochemistry & Biotechnology
Arthitaya Kawee-Ai, Nuntinee Ritthibut, Apisit Manassa, Churairat Moukamnerd, Thunnop Laokuldilok, Suthat Surawang, Sutee Wangtueai, Yuthana Phimolsiripol, Joe M Regenstein, Phisit Seesuriyachan
Prebiotic substances are extracted from various plant materials or enzymatic hydrolysis of different substrates. The production of fructo-oligosaccharide and inulo-oligosaccharide was carried out by applying two substrates, sucrose and inulin; oligosaccharide yields were maximized using central composite design to evaluate that the parameters influencing oligosaccharide production. Inulin from Jerusalem artichoke (5-15% w/v), sucrose (50-70% w/v), and inulinase from Aspergillus niger (2-7 U/g) were used as variable parameters for the optimization...
January 22, 2018: Preparative Biochemistry & Biotechnology
Mohan Kashid, Anand Ghosalkar
Fermentation of xylose from hydrolysate of acid treated corn cob by Pichia stipitis is inhibited by acetic acid and lignin derivatives. In the present study we have designed and implemented an immobilized cell culture for xylose to ethanol conversion from acid treated corn cob hydrolysate without removal of fermentation inhibitors. In this study, cultivations of suspended and immobilized Pichia were compared in terms of ethanol yield and productivity to investigate whether the cell immobilization could improve resistance to inhibitors...
January 22, 2018: Preparative Biochemistry & Biotechnology
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