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Preparative Biochemistry & Biotechnology

Priscilla Macedo Lima Andrade, Luciana Baptista, Julyana Stoffel Britto, Ana Paula Trovatti Uetenabaro, Andréa Miura da Costa
A novel tannase and gallic acid-producing Penicillium rolfsii (CCMB 714) was isolated from cocoa leaves from the South of Bahia. The influence of nutritional sources and the simultaneous effect of parameters involved in the fermentation process were available. Tannase (9.97 U mL-1 ) and gallic acid (9 mg mL-1 ) production were obtained in 48 h by submerged fermentation in non-optimized conditions. Among the carbon sources, tested gallic acid and tannic acid showed the highest tannase production (p<...
July 24, 2018: Preparative Biochemistry & Biotechnology
Sivaranjana Paramasivan, Nagarajan E R, Rajini Nagarajan, Varada Rajulu Anumakonda, Hariram N
In the present study, cotton fabric nanocomposites with in situ generated copper nanoparticles (CuNPs) were prepared using Cassia alata leaf extract as reducing agent. The prepared cotton fabric nanocomposites were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscoy (SEM) techniques. The cotton fabric nanocomposites exhibited significant antibacterial activity against Escherichia coli bacteria. These nanocomposites retained the antibacterial activity even after 15 washes indicating the generation of permanent CuNPs in them...
July 17, 2018: Preparative Biochemistry & Biotechnology
Zahra-Beagom Mokhtari-Hosseini, Ashrafalsadat Hatamian-Zarmi, Jaber Mohammadnejad, Bahman Ebrahimi-Hosseinzadeh
Chitin and chitosan with unique properties and numerous applications can be produced from fungus. The production of chitin and chitosan from the mycelia of an Iranian Ganoderma lucidum was studied to improve cell growth and chitin productivity. Inoculum size and initial pH as two effective variables on the growth of G. lucidum and chitin production were optimized using response surface method (RSM) by central composite design (CCD). The results verified the significant effect of these two variables on the cell growth and chitin production...
July 17, 2018: Preparative Biochemistry & Biotechnology
Jinender Jain, Anil Kumar, Davender Singh, Bijender Singh
A novel protease-resistant and thermostable phytase from Bacillus subtilis subsp. subtilis JJBS250 was purified 36-fold to homogeneity with a combination of ammonium sulfate precipitation followed by Q-Sepharose and Sephadex G-50 chromatographic techniques. The estimated molecular mass of the purified phytase was 46 kDa by electrophoresis with optimal activity at pH 7.0 and 70 °C. About 19% of original activity was maintained at 80 °C for 10 min. Phytase activity was stimulated in presence of surfactants like Tween-20, Tween-80, and Triton X-100 and metal ions like Ca+2 , K+ , and Co+2 and it was inhibited by SDS and Mg+2 , Al+2 , and Fe+2 ...
July 17, 2018: Preparative Biochemistry & Biotechnology
Arunangshu Das, Neetu Tyagi, Anita Verma, Sarfaraz Akhtar, Krishna J Mukherjee
L-Tyrosine which is one of the terminal metabolites of highly regulated aromatic amino-acid biosynthesis pathway in Escherichia coli is a precursor for synthesis of L-Dopa. In this study, we report over production of L-Dopa by enhancing expression of rate limiting isoenzyme of shikimate kinase (aroL), chorismate synthase (aroC), aromatic-amino-acid aminotransferase (tyrB) and 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) form a plasmid module harboring five enzymes under two inducible promoters converting shikimate to tyrosine...
July 17, 2018: Preparative Biochemistry & Biotechnology
Ekant Tamboli, Aditi Bhatnagar, Abha Mishra
The α-Amylase and α-glucosidase are two main enzymes involved in carbohydrate metabolism. This study was aimed at detecting alpha-amylase inhibitory activity from edible mushroom mycelia. Oyster mushroom was collected from a natural source, from Indian Institute of Technology (Banaras Hindu University) campus and was maintained in vitro in mycelial form. Chloroform, acetone, methanol, and water were used separately for extraction of an active constituent from mycelial cells grown, for 7 days, in potato dextrose broth...
July 17, 2018: Preparative Biochemistry & Biotechnology
Tales A Costa-Silva, Juan Carlos Flores-Santos, Rominne K B Freire, Michele Vitolo, Adalberto Pessoa-Jr
The efficacy of a simple laboratory method for cell disruption based on the glass bead stirring, sonication, osmotic shock, freezing and grinding, or use of solvents and detergents was assessed in this study, via measurements of the release of total protein and L-asparaginase activity. Three different microbial sources of L-asparaginase were used: Escherichia coli BL21 (DE3), Leucosporidium muscorum, and Aspergillus terreus (CCT 7693). This study adjusted and identified the best procedure for each kind of microorganism...
July 11, 2018: Preparative Biochemistry & Biotechnology
Le Chen, Ji-Liang Du, Yong-Jia Zhan, Jian-An Li, Ran-Ran Zuo, Shen Tian
Consolidated bioprocessing (CBP) strategy was developed to construct a cell-surface displayed consortium for heterologously expressing functional lignocellulytic enzymes. The reaction system composed of two engineered yeast strains: Y5/XynII-XylA (co-displaying two types of xylanases) and Y5/EG-CBH-BGL (co-displaying three types of cellulases). The immobilization of recombinant fusion proteins and their cell-surface accessibility of were analyzed by flow cytometry and immunofluorescence. The feasibility of consolidated bioprocessing by using pretreated corn stover (CS) as substrate for direct bioconversion was further investigated, and the synergistic activity and proximity effect between cellulases and xylanases on lignocelluloses degradation were also discussed in this work...
July 11, 2018: Preparative Biochemistry & Biotechnology
Sumitha Banu Jamaldheen, Kedar Sharma, Aruna Rani, Vijayanand S Moholkar, Arun Goyal
This study compares different types of pretreatment methods, such as thermal pretreatment at 120 °C, autoclaving, microwaving and ultrasonication in the presence of water, dilute acid (1% H2 SO4 ) or dilute alkali (1% NaOH) on Sorghum stalk with respect to the holocellulose and Acid Detergent/Insoluble Lignin content. Among all the methods, pretreatment with 1% NaOH along with autoclaving at 121 °C and 15 psi for 30 min was the most effective method for Sorghum stalk. Fourier Transform Infra-Red spectroscopy analysis of this pretreated biomass showed the removal of lignin and Field Emission Scanning Electron Microscope analysis displayed enhanced surface roughness...
July 9, 2018: Preparative Biochemistry & Biotechnology
Agbaje Lateef, Bolaji I Folarin, Suliat M Oladejo, Paul O Akinola, Lorika S Beukes, Evariste B Gueguim-Kana
Phytosynthesis of silver nanoparticles (AgNPs) using leaf extract of Petiveria alliacea (PA) was the focus of this research work. The PA-AgNPs were characterized by UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and selected area electron diffraction (SAED) study. Studies were made on the AgNPs for antibacterial, antifungal, anticoagulant, free-radical scavenging, and hydrogen peroxide scavenging activities. The crystalline PA-AgNPs were monodispersed, with a size range of 16...
June 29, 2018: Preparative Biochemistry & Biotechnology
Elaheh Khosrowabadi, Zeinab Takalloo, Reza H Sajedi, Khosro Khajeh
Escherichia coli is a common host that is widely used for producing recombinant proteins. However, it is a simple approach for production of heterologous proteins; the major drawbacks in using this organism include incorrect protein folding and formation of disordered aggregated proteins as inclusion bodies. Co-expression of target proteins with certain molecular chaperones is a rational approach for this problem. Aequorin is a calcium-activated photoprotein that is often prone to form insoluble inclusion bodies when overexpressed in E...
June 29, 2018: Preparative Biochemistry & Biotechnology
Yagmur Unver, Melike Yildiz, Deryanur Kilic, Mesut Taskin, Abdulhadi Firat, Hakan Askin
PinX1 encoded by a remarkable tumor suppressor gene and located in human chromosome 8p23 is known as telomerase inhibitor. In recent years, this protein has been of interest as clinically tumor suppressor. Pichia pastoris expression system is preferred to produce heterologous proteins and is suitable for industrial and research purposes. In the present study, human PinX1 gene (hPinX1) was cloned in E. coli One Shot TOP10 cells and overexpressed in P. pastoris strain X-33 intracellularly, using a strong AOX (alcohol oxidase) promoter...
June 29, 2018: Preparative Biochemistry & Biotechnology
Xiaoru Wang, Xiaorong Tian, Yuanjie Wu, Xiaofang Shen, Songbai Yang, Shaoxin Chen
Doxorubicin (DXR), which is produced by Streptomyces peucetius, is an important anthracycline-type antibiotic used for the treatment of various cancers. However, due to the low DXR productivity of wild-type S. peucetius, it is difficult to produce DXR by one-step fermentation. In this study, a DXR-resistance screening method was developed to screen for DXR high-producing mutants. Then, S. peucetius SIPI-11 was treated several times with UV and ARTP (atmospheric and room temperature plasma) to induce mutations...
June 25, 2018: Preparative Biochemistry & Biotechnology
Jhéssica C S Golveia, Mariângela F Santiago, Paulo T F Sales, Adilson Sartoratto, Alexandre N Ponezi, Douglas V Thomaz, Eric de Souza Gil, Maria Teresa F Bara
Bioremediation is a strategy to mitigate environmental impacts of hazardous pollutants from anthropogenic sources. Natural byproducts, including agroindustrial wastes (AW) can be used to induce enzyme biosynthesis, leading up to enhancement of pollutants degradation process. Therefore, this study aimed to evaluate the use of cupuaçu, Theobroma grandiflorum AW as Pycnoporus sanguineus Laccase (Lac) inducer in order to promote 17-α-ethinylestradiol (EE2) bioremediation. The macro and micro-nutrients levels of cupuaçu AWs were evaluated in order to establish further correlations with enzymatic biosynthesis induction...
June 25, 2018: Preparative Biochemistry & Biotechnology
Vikas U Kapase, Asha A Nesamma, Pannaga P Jutur
Optimizing the production of the high-value renewables such as OMEGAs through pathway engineering requires an in-depth understanding of the structure-function relationship of genes involved in the OMEGA biosynthetic pathways. In this preliminary study, our rationale is to identify and characterize the ∼221 putative genes involved in production of OMEGAs using bioinformatic analysis from the Streptophyte (plants), Chlorophyte (green algae), Rhodophyta (red algae), and Bacillariophyta (diatoms) lineages based on their phylogenomic profiling, conserved motif/domain organization and physico-chemical properties...
June 22, 2018: Preparative Biochemistry & Biotechnology
Paula Mirella Gomes Barbosa, Tobias Pereira de Morais, Cinthia Aparecida de Andrade Silva, Flávia Regina da Silva Santos, Nayara Fernanda Lisbo Garcia, G G Fonseca, Rodrigo Simões Ribeiro Leite, Marcelo Fossa da Paz
Invertases are used for several purposes; one among these is the production of fructooligosaccharides. The aim of this study was to biochemically characterize invertase from industrial Saccharomyces cerevisiae CAT-1 and Rhodotorula mucilaginosa isolated from Cerrado soil. The optimum pH and temperature were 4.0 and 70 °C for Rhodotorula mucilaginosa invertase and 4.5 and 50 °C for Saccharomyces cerevisiae invertase. The pH and thermal stability from 3.0 to 10.5 and 75 °C for R. mucilaginosa invertase, respectively...
June 22, 2018: Preparative Biochemistry & Biotechnology
Seyed Nezamedin Hosseini, Parisa Ghaisari, Shahram Sharifnia, Maryam Khatami, Amin Javidanbardan
In the downstream process of recombinant hepatitis B surface antigen (rHBsAg), nano-colloidal silica adsorbent (Aerosil-380) is one of the possible methods to separate the antigen from other main impurities partially. The current study aimed to maximize the adsorptive capacity of Aerosil-380 as well as rHBsAg recovery for large-scale production of recombinant hepatitis B vaccine. The experimental design methodology was used to optimize the eight critical parameters influencing the efficiency, rHBsAg recovery, of the adsorption-desorption process in the lab-scale...
June 22, 2018: Preparative Biochemistry & Biotechnology
Ngoc-Thanh Thi Nguyen, Soi Yun, Dong Woo Lim, E K Lee
'Shielding' effect of a conjugated PEG molecule could cause a change in the electrostatic interaction characteristics of a PEGylate. We investigated how PEG chain length (or molecular weight) alters the electrostatic interaction potential of exenatide variants using their mono-PEGylates in a branched and linear form as model PEGylates. First, we performed the experiments to demonstrate the elution time changes of the mono-PEGylates conjugated with various MW PEGs (5, 10, 20, and 40 kD) using cation exchange chromatography (HiTrap® SP) at various pHs (2...
June 22, 2018: Preparative Biochemistry & Biotechnology
Jacopo Troisi, Steven Symes, David Adair, Angelo Colucci, Sonia Elisa Prisco, Carmen Imma Aquino, Immacolata Vivone, Maurizio Guida, Sean Richards
Analysis of the human placenta metabolome has great potential to advance the understanding of complicated pregnancies and deleterious fetal outcomes in remote populations, but samples preparation can present unique challenges. Herein, we introduce oven-drying as a simple and widely available method of sample preparation that will facilitate investigations of the placental metabolome from remote and under-studied populations. Placentae from complicated and uncomplicated pregnancies were prepared in three ways (oven-dried at 60 °C, fresh, lyophilized) for metabolome analysis via gas chromatography-mass spectrometry (GC-MS)...
June 22, 2018: Preparative Biochemistry & Biotechnology
Ignacio Niizawa, Brenda Yanina Espinaco, Jorge Rodrigo Leonardi, Josué Miguel Heinrich, Guillermo Adrián Sihufe
The study of microalgal culture has been growing in recent decades, because the cellular structure of microalgae has diverse highly valuable metabolites that have attract attention of numerous companies and research groups. The pigment astaxanthin is considered one of the most powerful antioxidants in nature. The microalga Haematococcus pluvialis was proposed as one of the best natural astaxanthin sources, because it can accumulate high amount of the pigment. In this work, we studied different stress treatments on H...
June 22, 2018: Preparative Biochemistry & Biotechnology
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