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Journal of Biomedical Optics

Verónica Sorgato, Michel Berger, Charlotte Emain, Christine Vever-Bizet, Jean-Marc Dinten, Geneviève Bourg-Heckly, Anne Planat-Chrétien
To approach wide-field optical properties quantification in real heterogeneous biological tissue, we developed a Dual-Step setup that couples a punctual diffuse reflectance spectroscopy (DRS) technique with multispectral imaging (MSI). The setup achieves wide-field optical properties assessment through an initial estimation of scattering with DRS, which is used to estimate absorption with MSI. The absolute quantification of optical properties is based on the ACA-Pro algorithm that has been adapted both for DRS and for MSI...
September 2018: Journal of Biomedical Optics
Steffen Nothelfer, Florian Bergmann, André Liemert, Dominik Reitzle, Alwin Kienle
A method to correct for surface scattering in spatial frequency domain imaging (SFDI) is presented. The use of a modified analytical solution of the radiative transfer equation allows calculation of the reflectance and the phase of a rough semi-infinite geometry so that both spatial frequency domain reflectance and phase can be applied for precise retrieval of the bulk optical properties and the surface scattering. For validation of the method, phantoms with different surface roughness were produced. Contrarily, with the modified theory, it was possible to dramatically reduce systematic errors due to surface scattering...
September 2018: Journal of Biomedical Optics
Carole K Hayakawa, Kavon Karrobi, Vivian Pera, Darren Roblyer, Vasan Venugopalan
We present a Monte Carlo (MC) method to determine depth-dependent probability distributions of photon visitation and detection for optical reflectance measurements performed in the spatial frequency domain (SFD). These distributions are formed using an MC simulation for radiative transport that utilizes a photon packet weighting procedure consistent with the two-dimensional spatial Fourier transform of the radiative transport equation. This method enables the development of quantitative metrics for SFD optical sampling depth in layered tissue and its dependence on both tissue optical properties and spatial frequency...
September 2018: Journal of Biomedical Optics
Joseph P Angelo, Sez-Jade Chen, Marien Ochoa, Ulas Sunar, Sylvain Gioux, Xavier Intes
Diffuse optical imaging probes deep living tissue enabling structural, functional, metabolic, and molecular imaging. Recently, due to the availability of spatial light modulators, wide-field quantitative diffuse optical techniques have been implemented, which benefit greatly from structured light methodologies. Such implementations facilitate the quantification and characterization of depth-resolved optical and physiological properties of thick and deep tissue at fast acquisition speeds. We summarize the current state of work and applications in the three main techniques leveraging structured light: spatial frequency-domain imaging, optical tomography, and single-pixel imaging...
September 2018: Journal of Biomedical Optics
Hein de Hoop, Heechul Yoon, Kelsey Kubelick, Stanislav Emelianov
This study explores photoacoustic (PA) speckle tracking to characterize flow as an alternative to ultrasound (US) speckle tracking or current PA flow imaging methods. In cases where tracking of submicrometer particles is required, the US signal-to-noise ratio and contrast might be low due to limited reflectivity of subwavelength size targets at low concentrations. However, it may be possible to perform more accurate velocimetry using PAs due to different contrast mechanisms utilized in PA imaging. Here, we introduce a PA-based speckle tracking method that overcomes the directional dependence of Doppler imaging and the limited field of view of current correlation-based methods used in PA flow imaging...
September 2018: Journal of Biomedical Optics
Shadi Masoumi, Mohammad Ali Ansari, Ezeddin Mohajerani, Elina A Genina, Valery V Tuchin
Recently, compression optical clearing (OC) was applied to detect dermal carotenoid using reflection spectroscopy. To enhance the precision and accuracy of reflection spectroscopy to better detect the spectral absorption of beta-carotene inside biological phantom, here, we simultaneously use compression and immersion OC using dimethyl sulfoxide. In addition, we analytically extract the absorption coefficient of beta-carotene using diffuse reflectance spectroscopy (as an analytical OC). Our results show that the presented analytical OC can be applied alone as a noninvasive method to measure cutaneous chromophores at deep tissues...
September 2018: Journal of Biomedical Optics
Ivana Maria Zaccara, Letícia Boldrin Mestieri, Maria Stella Moreira, Fabiana Soares Grecca, Manoela Domingues Martins, Patrícia Maria Poli Kopper
Photobiomodulation therapy (PBM) has shown positive effects on stem cell differentiation in monolayer cell culture model, but little is known about its effect on three-dimensional (3-D) agarose gel culture. This study evaluated the PBM effect of human dental pulp stem cells (hDPSCs) differentiation and phosphatase alkaline activity (ALP) using an agarose 3-D model under different nutritional conditions. hDPSCs were characterized and seeded on a 0.3% agarose gel layer with different media (osteogenic, adipogenic, chondrogenic) and were assigned into four groups: control 10% fetal bovine serum (FBS), control 5% FBS, PBM 10% FBS, and PBM 5% FBS...
September 2018: Journal of Biomedical Optics
Shu-Ichiro Fukushima, Makoto Yonetsu, Takeshi Yasui
Skin wrinkling is a typical symptom of cutaneous photoaging; however, the skin wrinkling depends on not only the actual age but also exposure history to ultraviolet B (UVB) rays in individuals. Therefore, there is considerable need for its assessment technique in vivo in skin cosmetics and antiaging dermatology. Wrinkles always appear as linear grooves in the skin, and dermal collagen fibers play an important role to determine the morphology and mechanical properties of the skin. Therefore, an optical probe sensitive to dermal collagen fiber and its orientation will be useful...
September 2018: Journal of Biomedical Optics
Kamila Moor, Yusuke Terada, Akinori Taketani, Hiroko Matsuyoshi, Kiyoshi Ohtani, Hidetoshi Sato
Virus infection of a human cell was determined only 3 h after invagination. We used viral vector Ad-CMV-control (AdC), which lacks the E1 gene coding for early polypeptide 1 (E1). AdC can replicate in human embryonic kidney 293 (HEK293) cells into which the E1 gene has been transfected. According to partial least-square regression discriminant analysis, it was assumed that two kinds of reaction take place in the cell during viral invasion. The first response of the cell was determined 3 h after the virus invasion, and the second one was determined ∼9  h later...
September 2018: Journal of Biomedical Optics
Lydia Sauer, Karl M Andersen, Chantal Dysli, Martin S Zinkernagel, Paul S Bernstein, Martin Hammer
Autofluorescence-based imaging techniques have become very important in the ophthalmological field. Being noninvasive and very sensitive, they are broadly used in clinical routines. Conventional autofluorescence intensity imaging is largely influenced by the strong fluorescence of lipofuscin, a fluorophore that can be found at the level of the retinal pigment epithelium. However, different endogenous retinal fluorophores can be altered in various diseases. Fluorescence lifetime imaging ophthalmoscopy (FLIO) is an imaging modality to investigate the autofluorescence of the human fundus in vivo...
September 2018: Journal of Biomedical Optics
Zhaojun Wang, Yanan Cai, Yansheng Liang, Dan Dan, Baoli Yao, Ming Lei
Point spread function (PSF) engineering has met with lots of interest in various optical imaging techniques, including super-resolution microscopy, microparticle tracking, and extended depth-of-field microscopy. The intensity distributions of the modified PSFs often suffer from deteriorations caused by system aberrations, which greatly degrade the image contrast, resolution, or localization precision. We present an aberration correction method using a spiral-phase-based double-helix PSF as an aberration indicator, which is sensitive and quantitatively correlated to the spherical aberration, coma, and astigmatism...
September 2018: Journal of Biomedical Optics
Brian W Pogue
This editorial offers some ways to think about how best to position a research group for funding, by examining the parallels between what is needed for translational grants versus industry start-ups.
August 2018: Journal of Biomedical Optics
Bernhard Baumann, Marco Augustin, Antonia Lichtenegger, Danielle Harper, Martina Muck, Pablo Eugui, Andreas Wartak, Michael Pircher, Christoph Hitzenberger
Polarization-sensitive optical coherence tomography (PS-OCT) enables noninvasive, high-resolution imaging of tissue polarization properties. In the anterior segments of human eyes, PS-OCT allows the visualization of birefringent and depolarizing structures. We present the use of PS-OCT for imaging the murine anterior eye. Using a spectral domain PS-OCT setup operating in the 840-nm regime, we performed in vivo volumetric imaging in anesthetized C57BL/6 mice. The polarization properties of murine anterior eye structures largely replicated those known from human PS-OCT imagery, suggesting that the mouse eye may also serve as a model system under polarization contrast...
August 2018: Journal of Biomedical Optics
Daqing Piao, Alan Hawxby, Harlan Wright, Erin M Rubin
Solid-organ transplant is one of the most complex areas of modern medicine involving surgery. There are challenging opportunities in solid-organ transplant, specifically regarding the deficiencies in pathology workflow or gaps in pathology support, which may await alleviations or even de novo solutions, by means of point-of-care, or point-of-procedure optical biomarkers. Focusing the discussions of pathology workflow on donor liver assessment, we analyze the undermet need for intraoperative, real-time, and nondestructive assessment of the donor injuries (such as fibrosis, steatosis, and necrosis) that are the most significant predictors of post-transplant viability...
August 2018: Journal of Biomedical Optics
Ling-Hsuan Tsai, Po Nien Yang, Chung-Chih Wu, Hoang Yan Lin
In optical coherence tomography (OCT) systems, to precisely obtain the scattering properties of samples is an essential issue in diagnostic applications. Especially with a higher density turbid medium, the light interferes among the adjacent scatters. Combining an OCT experiment with the finite-difference time-domain simulation method, the multiple scattering effect is shown to affect the scattering properties of medium depending on the interparticle spacing. The far-field scattering phase function of scatters with various diameters was simulated to further analyze the corresponding anisotropy factors, which can be introduced into the extended Huygens-Fresnel theory to find the scattering coefficient of measured samples...
August 2018: Journal of Biomedical Optics
Ashton S Hemphill, Yuecheng Shen, Jeeseong Hwang, Lihong V Wang
Digital optical phase conjugation (DOPC) enables many optical applications by permitting focusing of light through scattering media. However, DOPC systems require precise alignment of all optical components, particularly of the spatial light modulator (SLM) and camera, in order to accurately record the wavefront and perform playback through the use of time-reversal symmetry. We present a digital compensation technique to optimize the alignment of the SLM in five degrees of freedom, permitting focusing through thick scattering media with a thickness of 5 mm and transport scattering coefficient of 2...
August 2018: Journal of Biomedical Optics
Mohadeseh Hashemi, Bharadwaj Muralidharan, Meisam Omidi, Javad Mohammadi, Yahya Sefidbakht, Eun Song Kima, Hugh D C Smyth, Mohammad Shalbaf, Thomas E Milner
Photothermal therapy with various nanoparticles, as photothermal transducers, is a widely researched technique. A continuous wave (CW) laser is employed during this procedure. The therapeutic setup is slightly modified to measure the optical absorption cross-section of the graphene oxide (GO), by mitigating the effects of heat diffusion and light scattering. With an 808-nm CW laser setup modulated by a waveform modulation setup, the effect of nanoparticle size and composition of GO in water on optical absorption cross section is characterized...
August 2018: Journal of Biomedical Optics
Gage Greening, Ariel Mundo, Narasimhan Rajaram, Timothy J Muldoon
Diffuse reflectance spectroscopy (DRS) is a probe-based spectral biopsy technique used in cancer studies to quantify tissue reduced scattering (μs') and absorption (μa) coefficients and vary in source-detector separation (SDS) to fine-tune sampling depth. In subcutaneous murine tumor allografts or xenografts, a key design requirement is ensuring that the source light interrogates past the skin layer into the tumor without significantly sacrificing signal-to-noise ratio (target of ≥15  dB). To resolve this requirement, a DRS probe was designed with four SDSs (0...
August 2018: Journal of Biomedical Optics
Mathi Damodaran, Arjen Amelink, Johannes F de Boer
Retinal blood vessel oxygenation is considered to be an important marker for numerous eye diseases. Oxygenation is typically assessed by imaging the retinal vessels at different wavelengths using multispectral imaging techniques, where the choice of wavelengths will affect the achievable measurement accuracy. Here, we present a detailed analysis of the error propagation of measurement noise in retinal oximetry, to identify optimal wavelengths that will yield the lowest uncertainty in saturation estimation for a given measurement noise level...
August 2018: Journal of Biomedical Optics
Alexey N Bashkatov, Kirill V Berezin, Konstantin N Dvoretskiy, Maria L Chernavina, Elina A Genina, Vadim D Genin, Vyacheslav I Kochubey, Ekaterina N Lazareva, Alexander B Pravdin, Marina E Shvachkina, Polina A Timoshina, Daria K Tuchina, Dmitry D Yakovlev, Dmitry A Yakovlev, Irina Yu Yanina, Olga S Zhernovaya, Valery V Tuchin
Nowadays, dynamically developing optical (photonic) technologies play an ever-increasing role in medicine. Their adequate and effective implementation in diagnostics, surgery, and therapy needs reliable data on optical properties of human tissues, including skin. This paper presents an overview of recent results on the measurements and control of tissue optical properties. The issues reported comprise a brief review of optical properties of biological tissues and efficacy of optical clearing (OC) method in application to monitoring of diabetic complications and visualization of blood vessels and microcirculation using a number of optical imaging technologies, including spectroscopic, optical coherence tomography, and polarization- and speckle-based ones...
August 2018: Journal of Biomedical Optics
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