journal
https://read.qxmd.com/read/38434231/perspectives-on-label-free-microscopy-of-heterogeneous-and-dynamic-biological-systems
#1
JOURNAL ARTICLE
Dan L Pham, Amani A Gillette, Jeremiah Riendeau, Kasia Wiech, Emmanuel Contreras Guzman, Rupsa Datta, Melissa C Skala
SIGNIFICANCE: Advancements in label-free microscopy could provide real-time, non-invasive imaging with unique sources of contrast and automated standardized analysis to characterize heterogeneous and dynamic biological processes. These tools would overcome challenges with widely used methods that are destructive (e.g., histology, flow cytometry) or lack cellular resolution (e.g., plate-based assays, whole animal bioluminescence imaging). AIM: This perspective aims to (1) justify the need for label-free microscopy to track heterogeneous cellular functions over time and space within unperturbed systems and (2) recommend improvements regarding instrumentation, image analysis, and image interpretation to address these needs...
December 2025: Journal of Biomedical Optics
https://read.qxmd.com/read/38495527/3d-polarization-interference-holographic-histology-for-wavelet-based-differentiation-of-the-polycrystalline-component-of-biological-tissues-with-different-necrotic-states-forensic-applications
#2
JOURNAL ARTICLE
Alexander Ushenko, Alexander Dubolazov, Jun Zheng, Alexandra Litvinenko, Mykhaylo Gorsky, Yuriy Ushenko, Iryna Soltys, Olexander Salega, Zhebo Chen, Oleh Wanchuliak
SIGNIFICANCE: The interference-holographic method of phase scanning of fields of scattered laser radiation is proposed. The effectiveness of this method for the selection of variously dispersed components is demonstrated. This method made it possible to obtain polarization maps of biological tissues at a high level of depolarized background. The scale-selective analysis of such maps was used to determine necrotic changes in the optically anisotropic architectonics of biological tissues...
May 2024: Journal of Biomedical Optics
https://read.qxmd.com/read/38420109/study-on-anisotropy-orientation-due-to-well-ordered-fibrous-biological-microstructures
#3
JOURNAL ARTICLE
Zhidi Liu, Jiawei Song, Qiqi Fu, Nan Zeng, Hui Ma
SIGNIFICANCE: Most biological fibrous tissues have anisotropic optical characteristics, which originate from scattering by their fibrous microstructures and birefringence of biological macromolecules. The orientation-related anisotropic interpretation is of great value in biological tissue characterization and pathological diagnosis. AIM: We focus on intrinsic birefringence and form birefringence in biological tissue samples. By observing and comparing the forward Mueller matrix of typical samples, we can understand the interpretation ability of orientation-related polarization parameters and further distinguish the sources and trends of anisotropy in tissues...
May 2024: Journal of Biomedical Optics
https://read.qxmd.com/read/38328279/mueller-matrix-based-characterization-of-cervical-tissue-sections-a-quantitative-comparison-of-polar-and-differential-decomposition-methods
#4
JOURNAL ARTICLE
Nishkarsh Kumar, Jeeban Kumar Nayak, Asima Pradhan, Nirmalya Ghosh
SIGNIFICANCE: Quantitative optical polarimetry has received considerable recent attention owing to its potential for being an efficient diagnosis and characterizing tool with potential applications in biomedical research and various other disciplines. In this regard, it is crucial to validate various Mueller matrix (MM) decomposition methods, which are utilized to extract and quantify the intrinsic individual polarization anisotropy properties of various complex optical media. AIM: To quantitatively compare the performance of both polar and differential MM decomposition methods for probing the structural and morphological changes in complex optical media through analyzing their intrinsic individual polarization parameters, which are extracted using the respective decomposition algorithms...
May 2024: Journal of Biomedical Optics
https://read.qxmd.com/read/38282917/speculum-free-portable-preterm-imaging-system
#5
JOURNAL ARTICLE
Tananant Boonya-Ananta, Mariacarla Gonzalez, Ajmal Ajmal, Vinh Nguyen Du Le, Edward DeHoog, Michael J Paidas, Arumugam Jayakumar, Jessica C Ramella-Roman
SIGNIFICANCE: Preterm birth is defined as a birth before 37 weeks of gestation and is one of the leading contributors to infant mortality rates globally. Premature birth can lead to life-long developmental impairment for the child. Unfortunately, there is a significant lack of tools to diagnose preterm birth risk, which limits patient care and the development of new therapies. AIM: To develop a speculum-free, portable preterm imaging system (PPRIM) for cervical imaging; testing of the PPRIM system to resolve polarization properties of birefringent samples; and testing of the PPRIM under an IRB on healthy, non-pregnant volunteers for visualization and polarization analysis of cervical images...
May 2024: Journal of Biomedical Optics
https://read.qxmd.com/read/38223746/analysis-of-polarization-features-of-human-breast-cancer-tissue-by-mueller-matrix-visualization
#6
JOURNAL ARTICLE
Thi-Thu-Hien Pham, Thao-Ngan Ngoc Quach, Quoc-Hoang-Quyen Vo
SIGNIFICANCE: Breast cancer ranks second in the world in terms of the number of women diagnosed. Effective methods for its early-stage detection are critical for facilitating timely intervention and lowering the mortality rate. AIM: Polarimetry provides much useful information on the structural properties of breast cancer tissue samples and is a valuable diagnostic tool. The present study classifies human breast tissue samples as healthy or cancerous utilizing a surface-illuminated backscatter polarization imaging technique...
May 2024: Journal of Biomedical Optics
https://read.qxmd.com/read/38089555/exploring-the-evolution-of-circular-polarized-light-backscattered-from-turbid-tissue-like-disperse-medium-utilizing-generalized-monte-carlo-modeling-approach-with-a-combined-use-of-jones-and-stokes-mueller-formalisms
#7
JOURNAL ARTICLE
Ivan Lopushenko, Oleksii Sieryi, Alexander Bykov, Igor Meglinski
SIGNIFICANCE: Phase retardation of circularly polarized light (CPL), backscattered by biological tissue, is used extensively for quantitative evaluation of cervical intraepithelial neoplasia, presence of senile Alzheimer's plaques, and characterization of biotissues with optical anisotropy. The Stokes polarimetry and Mueller matrix approaches demonstrate high potential in definitive non-invasive cancer diagnosis and tissue characterization. The ultimate understanding of CPL interaction with tissues is essential for advancing medical diagnostics, optical imaging, therapeutic applications, and the development of optical instruments and devices...
May 2024: Journal of Biomedical Optics
https://read.qxmd.com/read/38077502/machine-learning-based-local-recurrence-prediction-in-colorectal-cancer-using-polarized-light-imaging
#8
JOURNAL ARTICLE
Anamitra Majumdar, Jigar Lad, Kseniia Tumanova, Stefano Serra, Fayez Quereshy, Mohammadali Khorasani, Alex Vitkin
SIGNIFICANCE: Current treatment for stage III colorectal cancer (CRC) patients involves surgery that may not be sufficient in many cases, requiring additional adjuvant systemic therapy. Identification of this latter cohort that is likely to recur following surgery is key to better personalized therapy selection, but there is a lack of proper quantitative assessment tools for potential clinical adoption. AIM: The purpose of this study is to employ Mueller matrix (MM) polarized light microscopy in combination with supervised machine learning (ML) to quantitatively analyze the prognostic value of peri-tumoral collagen in CRC in relation to 5-year local recurrence (LR)...
May 2024: Journal of Biomedical Optics
https://read.qxmd.com/read/38077501/evaluating-backscattering-polarized-light-imaging-microstructural-mapping-capabilities-through-neural-tissue-and-analogous-phantom-imaging
#9
JOURNAL ARTICLE
Justina Bonaventura, Kellys Morara, Rhea Carlson, Courtney Comrie, AnneLeigh Twer, Elizabeth Hutchinson, Travis W Sawyer
SIGNIFICANCE: Knowledge of fiber microstructure and orientation in the brain is critical for many applications. Polarized light imaging (PLI) has been shown to have potential for better understanding neural fiber microstructure and directionality due to the anisotropy in myelin sheaths surrounding nerve fibers of the brain. Continuing to advance backscattering based PLI systems could provide a valuable avenue for in vivo neural imaging. AIM: To assess the potential of backscattering PLI systems, the ability to resolve crossing fibers, and the sensitivity to fiber inclination and curvature are considered across different imaging wavelengths...
May 2024: Journal of Biomedical Optics
https://read.qxmd.com/read/38077500/incremental-residual-polarization-caused-by-aging-in-human-skin
#10
JOURNAL ARTICLE
Viktor Dremin, Elena Zharkikh, Ivan Lopushenko, Zbignevs Marcinkevics, Alexander Bykov, Igor Meglinski
SIGNIFICANCE: The study of the effect of aging on the optical properties of biological tissues, in particular polarization, is important in the development of new diagnostic approaches. AIM: This work aims to provide a comprehensive analysis of the factors and mechanisms that contribute to the alteration of skin polarization properties caused by aging, using polarization-sensitive hyperspectral imaging measurements and Monte Carlo simulation. APPROACH: Our investigation involved both experimental studies of in vivo human skin of volunteers of different ages and computational modeling that accounted for changes in the absorption and scattering properties of the skin model...
May 2024: Journal of Biomedical Optics
https://read.qxmd.com/read/38532927/structurally-constrained-and-pathology-aware-convolutional-transformer-generative-adversarial-network-for-virtual-histology-staining-of-human-coronary-optical-coherence-tomography-images
#11
JOURNAL ARTICLE
Xueshen Li, Hongshan Liu, Xiaoyu Song, Charles C Marboe, Brigitta C Brott, Silvio H Litovsky, Yu Gan
SIGNIFICANCE: There is a significant need for the generation of virtual histological information from coronary optical coherence tomography (OCT) images to better guide the treatment of coronary artery disease (CAD). However, existing methods either require a large pixel-wise paired training dataset or have limited capability to map pathological regions. AIM: The aim of this work is to generate virtual histological information from coronary OCT images, without a pixel-wise paired training dataset while capable of providing pathological patterns...
March 2024: Journal of Biomedical Optics
https://read.qxmd.com/read/38532926/combined-frequency-domain-near-infrared-spectroscopy-and-diffuse-correlation-spectroscopy-system-for-comprehensive-metabolic-monitoring-of-inspiratory-muscles-during-loading
#12
JOURNAL ARTICLE
Carlos A Gómez, Laurent Brochard, Ewan C Goligher, Dmitry Rozenberg, W Darlene Reid, Darren Roblyer
SIGNIFICANCE: Mechanical ventilation (MV) is a cornerstone technology in the intensive care unit as it assists with the delivery of oxygen in critically ill patients. The process of weaning patients from MV can be long and arduous and can lead to serious complications for many patients. Despite the known importance of inspiratory muscle function in the success of weaning, current clinical standards do not include direct monitoring of these muscles. AIM: The goal of this project was to develop and validate a combined frequency domain near-infrared spectroscopy (FD-NIRS) and diffuse correlation spectroscopy (DCS) system for the noninvasive characterization of inspiratory muscle response to a load...
March 2024: Journal of Biomedical Optics
https://read.qxmd.com/read/38515831/full-aperture-extended-depth-oblique-plane-microscopy-through-dynamic-remote-focusing
#13
JOURNAL ARTICLE
Paolo Pozzi, Vipin Balan, Alessia Candeo, Alessia Brix, Anna Silvia Pistocchi, Cosimo D'Andrea, Gianluca Valentini, Andrea Bassi
SIGNIFICANCE: The reprojection setup typical of oblique plane microscopy (OPM) limits the effective aperture of the imaging system, and therefore its efficiency and resolution. Large aperture system is only possible through the use of custom specialized optics. A full-aperture OPM made with off the shelf components would both improve the performance of the method and encourage its widespread adoption. AIM: To prove the feasibility of an OPM without a conventional reprojection setup, retaining the full aperture of the primary objective employed...
March 2024: Journal of Biomedical Optics
https://read.qxmd.com/read/38487259/compact-simultaneous-label-free-autofluorescence-multi-harmonic-microscopy-for-user-friendly-photodamage-monitored-imaging
#14
JOURNAL ARTICLE
Geng Wang, Stephen A Boppart, Haohua Tu
SIGNIFICANCE: Label-free nonlinear optical microscopy has become a powerful tool for biomedical research. However, the possible photodamage risk hinders further clinical applications. AIM: To reduce these adverse effects, we constructed a new platform of simultaneous label-free autofluorescence multi-harmonic (SLAM) microscopy, featuring four-channel multimodal imaging, inline photodamage monitoring, and pulse repetition-rate tuning. APPROACH: Using a large-core birefringent photonic crystal fiber for spectral broadening and a prism compressor for pulse pre-chirping, this system allows users to independently adjust pulse width, repetition rate, and energy, which is useful for optimizing imaging conditions towards no/minimal photodamage...
March 2024: Journal of Biomedical Optics
https://read.qxmd.com/read/38481479/skin-characterization-of-diabetes-mellitus-revealed-by-polarization-sensitive-optical-coherence-tomography-imaging
#15
JOURNAL ARTICLE
Wei Feng, Lisi Wang, Chun-Jie Liu, Chao Zhang
SIGNIFICANCE: Diabetes can lead to the glycation of proteins and dysfunction of skin collagen. Skin lesions are a prevalent clinical symptom of diabetes mellitus (DM). Early diagnosis and assessing the efficacy of treatment for DM are crucial for patient health management. However, performing a non-invasive skin assessment in the early stages of DM is challenging. AIM: By using the polarization-sensitive optical coherent tomography (PS-OCT) imaging technique, it is possible to noninvasively assess the skin changes caused by diabetes...
March 2024: Journal of Biomedical Optics
https://read.qxmd.com/read/38476221/continuous-monitoring-of-endotracheal-tube-position-with-near-infrared-light
#16
JOURNAL ARTICLE
Tongtong Lu, Pawjai Khampang, Ahmed Beydoun, Anna Berezovsky, Rebecca Rohde, Wenzhou Hong, Bing Yu, Joseph E Kerschner
SIGNIFICANCE: Endotracheal intubation is a common approach for airway management in critically ill patients. However, the position of the endotracheal tube (ETT) may be altered during the procedure due to head movements. Accidental displacement or dislodge of the ETT may reduce the airflow, leading to moderate to severe complications, and in some cases even fatality. Therefore, timely detection of changes in ETT position in the trachea is critical to ensure immediate and intermediate interventions to maintain the ETT in the proper position...
March 2024: Journal of Biomedical Optics
https://read.qxmd.com/read/38476220/deep-learning-approach-to-stratified-reconstructions-of-tissue-absorption-and-scattering-in-time-domain-spatial-frequency-domain-imaging
#17
JOURNAL ARTICLE
Yaru Zhang, Wenxing Bai, Yihan Dong, Mai Dan, Dongyuan Liu, Feng Gao
SIGNIFICANCE: The conventional optical properties (OPs) reconstruction in spatial frequency domain (SFD) imaging, like the lookup table (LUT) method, causes OPs aliasing and yields only average OPs without depth resolution. Integrating SFD imaging with time-resolved (TR) measurements enhances space-TR information, enabling improved reconstruction of absorption (<mml:math xmlns:mml="https://www.w3.org/1998/Math/MathML"><mml:mrow><mml:msub><mml:mi>μ</mml:mi><mml:mi>a</mml:mi></mml:msub></mml:mrow></mml:math>) and reduced scattering (<mml:math xmlns:mml="https://www...
March 2024: Journal of Biomedical Optics
https://read.qxmd.com/read/38476219/can-diffuse-reflectance-spectroscopy-identify-shuntodynia-in-pediatric-hydrocephalus-patients
#18
JOURNAL ARTICLE
Olivia Kline, Karthik Vishwanath, Boyd Colbrunn, Andrew Peachman, Jing Zhang, Sudhakar Vadivelu
SIGNIFICANCE: Shuntodynia is patient reported pain at the site of the implanted ventriculoperitoneal (VP) shunt. Pediatric hydrocephalus requiring shunt placement is a chronic and prevalent standard of care treatment and requires lifetime management. Shuntodynia is a subjective measure of shunt dysfunction. Quantitative, white-light tissue spectroscopy could be used to objectively identify this condition in the clinic. AIM: Pediatric subjects were recruited for optical sensing during routine clinical follow-up visits, post-VP shunt implantations...
March 2024: Journal of Biomedical Optics
https://read.qxmd.com/read/38444669/non-invasive-blood-glucose-estimation-method-based-on-the-phase-delay-between-oxy-and-deoxyhemoglobin-using-visible-and-near-infrared-spectroscopy
#19
JOURNAL ARTICLE
Tomoya Nakazawa, Rui Sekine, Masato Kitabayashi, Yu Hashimoto, Anna Ienaka, Keiji Morishita, Takeo Fujii, Masaki Ito, Fumie Matsushita
SIGNIFICANCE: Many researchers have attempted to estimate blood glucose levels (BGLs) noninvasively using near-infrared (NIR) spectroscopy. However, the optical absorption change induced by blood glucose is weak in the NIR region and often masked by interference from other components such as water and hemoglobin. AIM: Instead of using direct optical absorption by glucose, this study proposes an index calculated from oxy- and deoxyhemoglobin signals that shows a good correlation with BGLs while using conventional visible and NIR spectroscopy...
March 2024: Journal of Biomedical Optics
https://read.qxmd.com/read/38440101/fluorescence-guided-surgery-imaging-systems-for-breast-cancer-identification-a-systematic-review
#20
REVIEW
Martha S Kedrzycki, Hazel T W Chon, Maria Leiloglou, Vadzim Chalau, Daniel R Leff, Daniel S Elson
SIGNIFICANCE: Breast-conserving surgery (BCS) is limited by high rates of positive margins and re-operative interventions. Fluorescence-guided surgery seeks to detect the entire lesion in real time, thus guiding the surgeons to remove all the tumor at the index procedure. AIM: Our aim was to identify the optimal combination of a camera system and fluorophore for fluorescence-guided BCS. APPROACH: A systematic review of medical databases using the terms "fluorescence," "breast cancer," "surgery," and "fluorescence imaging" was performed...
March 2024: Journal of Biomedical Optics
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