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Helicobacter

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https://www.readbyqxmd.com/read/28066960/consensus-on-the-clinical-management-screening-to-treat-and-surveillance-of-helicobacter-pylori-infection-to-improve-gastric-cancer-control-on-a-nationwide-scale
#1
Bor-Shyang Sheu, Ming-Shiang Wu, Cheng-Tang Chiu, Jing-Chuan Lo, Deng-Chyang Wu, Jyh-Ming Liou, Chun-Ying Wu, Hsiu-Chi Cheng, Yi-Chia Lee, Ping-I Hsu, Chun-Chao Chang, Wei-Lun Chang, Jaw-Town Lin
BACKGROUND: Previous international consensus statements provided general policies for the management of Helicobacter pylori infection. However, there are geographic differences in the prevalence and antimicrobial resistance of H. pylori, and in the availability of medications and endoscopy. Thus, nationwide or regional consensus statements are needed to improve control of H. pylori infection and gastric cancer. MATERIALS AND METHODS: This consensus statement for management of H...
January 8, 2017: Helicobacter
https://www.readbyqxmd.com/read/28029200/the-influence-of-helicobacter-pylori-on-the-ethnic-distribution-of-esophageal-eosinophilia
#2
Amnon Sonnenberg, Evan S Dellon, Kevin O Turner, Robert M Genta
BACKGROUND: Environmental factors associated with ethnicity may contribute to the occurrence of eosinophilic esophagitis. Our study aimed to investigate the influence of Helicobacter pylori on the ethnic variation of esophageal eosinophilia in a large national sample of patients undergoing esophago-gastro-duodenoscopy. METHODS: The Miraca Life Sciences Database is an electronic repository of histopathologic patient records. A case-control study evaluated the influence of ethnicity on the occurrence of esophageal eosinophilia and how age, gender, and histologic diagnosis of H...
December 28, 2016: Helicobacter
https://www.readbyqxmd.com/read/28029188/a-novel-isolation-protocol-and-probe-based-rt-pcr-for-diagnosis-of-gastric-infections-with-the-zoonotic-pathogen-helicobacter-suis
#3
Caroline Blaecher, Eva Bauwens, Alfred Tay, Fanny Peters, Sylvia Dobbs, John Dobbs, André Charlett, Richard Ducatelle, Freddy Haesebrouck, Annemieke Smet
BACKGROUND: Helicobacter suis is a very fastidious microorganism associated with gastritis, gastric ulcers, and mucosa-associated lymphoid tissue lymphoma in humans. In vitro isolation of this agent from human patients has so far been unsuccessful. MATERIALS AND METHODS: A probe-based real-time PCR (RT-PCR) for the rapid detection of H. suis in gastric biopsies was developed. Secondly, a mouse-passage-based protocol was optimized for isolation of low numbers of viable H...
December 28, 2016: Helicobacter
https://www.readbyqxmd.com/read/27990709/the-importance-of-th22-and-tc22-cells-in-the-pathogenesis-of-helicobacter-pylori-associated-gastric-diseases
#4
Seyedeh Azra Shamsdin, Abdolvahab Alborzi, Manoochehr Rasouli, Abbas Ghaderi, Kamran B Lankrani, Seyed Mohsen Dehghani, Gholam Reza Pouladfar
BACKGROUND: An association exists between Helicobacter pylori (H. pylori), peptic ulcers, gastritis, and sometimes gastric carcinomas. Th22 cells have protective and inflammatory roles in defense against microbes. AIM: We investigated the frequencies of Th22, Tc22, Th22/17, and Tc22/17 cells in addition to the changes in levels of cytokines IL-22, IL-6, IL-23, TNF-α, IL-1β, and TGF-β in sera from patients with H. pylori-associated gastritis, and peptic ulcer, and in uninfected patients...
December 19, 2016: Helicobacter
https://www.readbyqxmd.com/read/27933701/ccr2-mediates-helicobacter-pylori-induced-immune-tolerance-and-contributes-to-mucosal-homeostasis
#5
Xia Sun, Min Zhang, Mohamad El-Zaatari, Gray B Huffnagle, John Y Kao
BACKGROUND: We previously demonstrated that H. pylori infection leads to increased induction of regulatory T cells in local and systemic immune compartments. Here, we investigate the role of CCR2 in the tolerogenic programing of dendritic cells in a mouse model of H. pylori infection. MATERIALS AND METHODS: CCR2 deficient (CCR2KO) mice and wild-type (Wt) mice infected with H. pylori SS1 strain were analyzed by qPCR and FACS analysis. In vitro, bone marrow-derived DC on day 6 from CCR2KO and Wt mice cocultured with or without H...
December 8, 2016: Helicobacter
https://www.readbyqxmd.com/read/27879042/high-antibiotic-resistance-of-helicobacter-pylori-and-its-effect-on-tailored-and-empiric-eradication-of-the-organism-in-lower-silesia-poland
#6
Stanisław Ferenc, Jan Gnus, Magdalena Kościelna, Małgorzata Kinda, Andriy Yarka, Luke Stewart, Wojciech Witkiewicz
BACKGROUND: At present, the resistance to antibiotics is considered the most important reason for Helicobacter pylori (HP) eradication failure. The aim of this study was to estimate the prevalence of antimicrobial resistance of HP strains and to evaluate tailored and empiric therapeutic regimens in patients with peptic ulcer disease associated with infection of this microorganism. MATERIALS AND METHODS: Between May 2011 and February 2013, 185 consecutive Polish patients with at least one positive Helicobacter pylori test (urease test, histopathologic examination, and/or culture) underwent eradication therapy...
November 23, 2016: Helicobacter
https://www.readbyqxmd.com/read/27786400/helicobacter-pylori-colonization-and-pregnancies-complicated-by-preeclampsia-spontaneous-prematurity-and-small-for-gestational-age-birth
#7
Wouter J den Hollander, Sarah Schalekamp-Timmermans, I Lisanne Holster, Vincent W Jaddoe, Albert Hofman, Henriëtte A Moll, Guillermo I Perez-Perez, Martin J Blaser, Eric A P Steegers, Ernst J Kuipers
BACKGROUND: Preeclampsia (PE), small for gestational age (SGA), and spontaneous preterm birth (PTB) each may be complications of impaired placental function in pregnancy. Although their exact pathogenesis is still unknown, certain infectious agents seem to play a role. Helicobacter pylori (H. pylori) colonization has been associated with increased risk for PE. Our aim was to assess the association between H. pylori colonization and PE, SGA, and PTB. MATERIAL AND METHODS: We measured IgG anti-H...
October 27, 2016: Helicobacter
https://www.readbyqxmd.com/read/27785853/helicobacter-pylori-infection-and-its-related-factors-in-junior-high-school-students-in-nagano-prefecture-japan
#8
Yoshiko Nakayama, Yingsong Lin, Minoru Hongo, Hiroya Hidaka, Shogo Kikuchi
BACKGROUND: There have been few reports on Helicobacter pylori (H. pylori) infection in asymptomatic Japanese children and adolescents. We hypothesized that the prevalence of H. pylori infection is very low among Japanese children and that clinical variables such as serum pepsinogen and iron levels are associated with H. pylori infection. MATERIALS AND METHODS: We conducted a cross-sectional analysis of a sample of 454 junior high school students aged 12-15 years in four areas in Nagano Prefecture...
October 27, 2016: Helicobacter
https://www.readbyqxmd.com/read/27214853/detection-of-helicobacter-pylori-by-real-time-pcr-for-16s-rrna-in-stools-of-noninfected-healthy-children-using-elisa-antigen-stool-test-as-the-gold-standard
#9
Sergio George, Nora Mamani, Yalda Lucero, Juan Pablo Torres, Mauricio Farfán, Anne J Lagomarcino, Andrea Orellana, Miguel O'Ryan
BACKGROUND: We previously detected Helicobacter pylori infection by stool antigen ELISA assay in 33-41% of asymptomatic Chilean children between 2-3 years of age, of which 11-20% had a transient infection and 21-22% a persistent infection. A total of 88% of ELISA-positive samples were also rtPCR positive, while 37/133 (33%) of ELISA-negative stool samples were rtPCR positive. The significance of a ELISA-negative/rtPCR-positive sample requires clarification. We aimed to determine whether rtPCR is able to detect persistent infections not detected by ELISA...
December 2016: Helicobacter
https://www.readbyqxmd.com/read/27191536/effect-of-helicobacter-pylori-eradication-and-abo-genotype-on-gastric-cancer-development
#10
Sooyeon Oh, Nayoung Kim, Jin-Won Kwon, Cheol Min Shin, Yoon Jin Choi, Dong Ho Lee, Hyun Chae Jung
BACKGROUND: Evidence is lacking regarding how Helicobacter pylori infection status, eradication history, and ABO blood type affect the development of gastric cancer (GC) given the multifactorial and distinctive etiology according to cancer location (noncardia vs cardia) and histologic type (intestinal vs diffuse-type). We evaluated the effect of H. pylori infection status incorporated with H. pylori eradication history and ABO genotype on GC development according to cancer location and histologic type...
December 2016: Helicobacter
https://www.readbyqxmd.com/read/27172813/routine-anti-helicobacter-immunohistochemical-staining-is-significantly-superior-to-reflex-staining-protocols-for-the-detection-of-helicobacter-in-gastric-biopsy-specimens
#11
Richard H Lash, Robert M Genta
BACKGROUND: The role of special stains in the detection of H. pylori is controversial; some pathologists claim that hematoxylin and eosin staining alone is adequate to detect bacteria, while others maintain that immunohistochemical staining must be used on all gastric biopsies. This study was designed to test the hypothesis that hematoxylin and eosin and histochemical special stains have similar sensitivity and that immunohistochemical staining only marginally enhances the detection rate of the organisms...
December 2016: Helicobacter
https://www.readbyqxmd.com/read/27172105/the-all-age-prevalence-of-helicobacter-pylori-infection-and-potential-transmission-routes-a-population-based-study
#12
Ragnar K Breckan, Eyvind J Paulssen, Anne Mette Asfeldt, Jan-Magnus Kvamme, Bjørn Straume, Jon Florholmen
BACKGROUND AND AIMS: Previous research on H. pylori epidemiology has mostly focused on adult populations. We have aimed to study H. pylori prevalence in all age groups including children and adolescents and to identify potential routes of transmission. METHODS: Subjects from all age groups (children 0-11 years, adolescents 12-17 years and adults ≥18 years of age), recruited from both an urban and a rural community in Northern Norway, were invited to provide stool samples for the diagnosis of H...
December 2016: Helicobacter
https://www.readbyqxmd.com/read/27098759/role-of-helicobacter-pylori-infection-in-body-height-of-adult-dyspeptic-patients
#13
Maria P Dore, Giovanni M Pes, Giovanni Sferlazzo, Giuseppina Marras, Gabrio Bassotti
INTRODUCTION: The prevalence of H. pylori infection is high in underdeveloped countries and is associated with growth retardation. In the first half of the 20th century, Sardinia was an underdeveloped region; however, more recent development resulted in a decline in H. pylori infection. Because body height is correlated with health and nutritional status in childhood, the association among H. pylori infection and height was explored. MATERIALS AND METHODS: A retrospective observational study was conducted involving patients undergoing endoscopy for dyspepsia from 2002 to 2012...
December 2016: Helicobacter
https://www.readbyqxmd.com/read/27061753/a-21-35-kda-mixed-protein-component-from-helicobacter-pylori-activates-mast-cells-effectively-in-chronic-spontaneous-urticaria
#14
Ran-Jing Tan, He-Qiang Sun, Wei Zhang, Han-Mei Yuan, Bin Li, Hong-Tao Yan, Chun-Hui Lan, Jun Yang, Zhuo Zhao, Jin-Jin Wu, Chao Wu
BACKGROUND: Helicobacter pylori (H. pylori) seem to involve in the etiology of chronic spontaneous urticaria (CSU). But studies of the pathogenic mechanism are very little. METHODS: In this study, we detected the serum-specific anti-H. pylori IgG and IgE antibodies in 211 CSU and 137 normal subjects by enzyme-linked immunosorbent assay (ELISA), evaluated the direct activation effects of H. pylori preparations and its protein components on human LAD2 mast cell line in vitro, and analyzed the specific protein ingredients and functions of the most effective H...
December 2016: Helicobacter
https://www.readbyqxmd.com/read/27061611/molecular-detection-of-h-pylori-using-adherent-gastric-mucous-to-biopsy-forceps
#15
Hiroshi Matsumoto, Akiko Shiotani, Hiroyuki Nishibayashi, Tomoari Kamada, Tomonari Kimura, Yoshinori Fujimura, Rui Nakato, Takahisa Murao, Minoru Fujita, Ken Haruma
BACKGROUND AND AIM: We assessed whether adherent gastric mucous to biopsy forceps instead of biopsy samples was suitable for the diagnosis of H. pylori infection. We confirmed the PCR methods to improve the diagnosis of H. pylori infection and clarithromycin (CAM) susceptibility. METHODS: Gastric mucous was obtained by gently scraping gastric mucosa using biopsy forceps in patients undergoing upper gastrointestinal (GI) endoscopy for PCR and rapid urease test (RUT)...
December 2016: Helicobacter
https://www.readbyqxmd.com/read/27060717/the-upregulation-of-traf1-induced-by-helicobacter-pylori-plays-an-antiapoptotic-effect-on-the-infected-cells
#16
Xiu-Kun Wan, Sheng-Ling Yuan, Hao-Xia Tao, Li-Peng Diao, Yan-Chun Wang, Cheng Cao, Chun-Jie Liu
BACKGROUND: Tumor necrosis factor receptor-associated factor 1 (TRAF1) is a member of the TRAF family and is dysregulated in diseases, such as atheroma, lymphoma, and solid tumors, but the role of TRAF1 in gastric cancer remains unknown. This study was aimed to investigate the role of TRAF1 in Helicobacter pylori (H. pylori)-related cell apoptosis and gastric carcinogenesis. MATERIALS AND METHODS: The mRNA and protein expression levels of TRAF1 were measured in a panel of gastric cancer cell lines and in H...
December 2016: Helicobacter
https://www.readbyqxmd.com/read/27060292/rabeprazole-minocycline-amoxicillin-and-bismuth-as-first-line-and-second-line-regimens-for-helicobacter-pylori-eradication
#17
Zhiqiang Song, Baojun Suo, Lingyun Zhang, Liya Zhou
BACKGROUND: Because of general unavailability of tetracycline, common adverse effects, and complicated administration, the clinical application of bismuth quadruple therapy often faces difficulties. Whether the combination of minocycline and amoxicillin can replace tetracycline and metronidazole for Helicobacter pylori eradication remains unclear. This study was to determine the efficacy, compliance, and safety of rabeprazole, minocycline, amoxicillin, and bismuth (RMAB) therapy as first-line and second-line regimens...
December 2016: Helicobacter
https://www.readbyqxmd.com/read/27006283/xenobiotic-pathway-gene-polymorphisms-associated-with-gastric-cancer-in-high-risk-mizo-mongoloid-population-northeast-india
#18
Souvik Ghatak, Ravi Prakash Yadav, Freda Lalrohlui, Payel Chakraborty, Soumee Ghosh, Sudakshina Ghosh, Madhusudan Das, Jeremy L Pautu, John Zohmingthanga, Nachimuthu Senthil Kumar
BACKGROUND: The aim of this study was to evaluate the risk of gastric cancer associated with individual or combined glutathione S-transferases (GSTs) polymorphism and their interaction with environmental factors. MATERIALS AND METHODS: Genotyping by PCR was carried out for 80 cases and controls each for GSTM1, GSTT1, and GSTP1 polymorphism and mapped for gene-environment association studies. The samples were subjected to pathogen detection and GSTP1 expression for analyzing their association with different genotypes...
December 2016: Helicobacter
https://www.readbyqxmd.com/read/27006167/prediagnostic-helicobacter-pylori-antibodies-and-colorectal-cancer-risk-in-an-elderly-caucasian-population
#19
Jennifer L Blase, Peter T Campbell, Susan M Gapstur, Michael Pawlita, Angelika Michel, Tim Waterboer, Lauren R Teras
BACKGROUND: Study results on overall seroprevalence of Helicobacter pylori and colorectal cancer risk have been inconsistent. However, one study found positive associations with antibodies to specific H. pylori proteins. To follow up on those findings, we assessed associations of 15 H. pylori specific proteins with colorectal cancer incidence in the prospective Cancer Prevention Study-II Nutrition Cohort. MATERIALS AND METHODS: Participants in this nested case-control study included 392 cases and 774 controls who were predominantly elderly (median age at blood draw: 71 years) and Caucasian (98%)...
December 2016: Helicobacter
https://www.readbyqxmd.com/read/27004488/effects-of-helicobacter-pylori-infection-on-the-expressions-and-functional-activities-of-human-duodenal-mucosal-bicarbonate-transport-proteins
#20
Guorong Wen, Hai Jin, Shili Deng, Jingyu Xu, Xuemei Liu, Rui Xie, Biguang Tuo
BACKGROUND: The mechanisms for Helicobacter pylori (H. pylori)-induced duodenal ulcerogenesis are not fully understood. In this study, we investigated the effects of H. pylori infection on the expressions and functional activities of human duodenal mucosal bicarbonate transport proteins and hope to further clarify the pathogenesis of H. pylori-associated duodenal ulcer. MATERIALS AND METHODS: The experiments were performed in the patients with H. pylori-associated duodenal ulcers, H...
December 2016: Helicobacter
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