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Miaoli Wu, Dan Rao, Yujun Zhu, Jing Wang, Wen Yuan, Yu Zhang, Ren Huang, Pengju Guo
BACKGROUND: Enterohepatic Helicobacter species (EHS) are widespread in rodent species around the world. Several studies have demonstrated that infection with EHS can interfere with the outcomes of animal experiments in cancer research and significantly influence the study results. Therefore, it is essential to establish a rapid detection and identification of EHS for biomedical research using laboratory rodents. Our study aimed to develop a rapid and sensitive method to detect and distinguish five enterohepatic Helicobacter species...
September 30, 2016: Helicobacter
Hiroshi Mihara, Nobuhiro Suzuki, Jibran Sualeh Muhammad, Sohachi Nanjo, Takayuki Ando, Haruka Fujinami, Shinya Kajiura, Ayumu Hosokawa, Toshiro Sugiyama
BACKGROUND: Helicobacter pylori (HP) infection induces methylation silencing of specific genes in gastric epithelium. Various stimuli activate the nonselective cation channel TRPV4, which is expressed in gastric epithelium where it detects mechanical stimuli and promotes ATP release. As CpG islands in TRPV4 are methylated in HP-infected gastric epithelium, we evaluated HP infection-dependent changes in TRPV4 expression in gastric epithelium. MATERIALS AND METHODS: Human gastric biopsy samples, a human gastric cancer cell line (AGS), and a normal gastric epithelial cell line (GES-1) were used to detect TRPV4 mRNA and protein expression by RT-PCR and Western blotting, respectively...
September 30, 2016: Helicobacter
Nur R Abdul Rahim, Jill Benson, Kathryn Grocke, Deeva Vather, Jessica Zimmerman, Tessa Moody, Lillian Mwanri
BACKGROUND: To determine the prevalence of Helicobacter pylori infection in the refugee population attending the Migrant Health Service, South Australia, identify demographic factors associated with infection and compare prevalence of infection in refugees with that of the nonrefugee population in Australia. MATERIALS AND METHODS: Cross-sectional study conducted between October 2010 and August 2013. Monoclonal stool antigen testing for H. pylori infection is performed as part of a comprehensive health assessment for newly arrived refugees...
September 22, 2016: Helicobacter
Antonio Ponzetto, Natale Figura
No abstract text is available yet for this article.
September 7, 2016: Helicobacter
Magali Garcia, Jean-Claude Chomel, Pascale Mustapha, Cong Tri Tran, Martine Garnier, Isabelle Paris, Nathalie Quellard, Julie Godet, Julie Cremniter, Annelise Bennaceur-Griscelli, Jean-Claude Lecron, Ali G Turhan, Christophe Burucoa, Charles Bodet
BACKGROUND: Human gastric mucosa shows continuous self-renewal via differentiation from stem cells that remain poorly characterized. METHODS: We describe an original protocol for culture of gastric stem/progenitor cells from adult human stomach. The molecular characteristics of cells were studied using TaqMan low-density array and qRT-PCR analyses using the well-characterized H1 and H9 embryonic stem cells as reference. Epithelial progenitor cells were challenged with H...
September 4, 2016: Helicobacter
Maria Harmati, Edina Gyukity-Sebestyen, Gabriella Dobra, Gabriella Terhes, Edit Urban, Gabor Decsi, Neda Mimica-Dukić, Marija Lesjak, Nataša Simin, Bernadett Pap, Istvan B Nemeth, Krisztina Buzas
BACKGROUND: Helicobacter pylori can cause many gastrointestinal and also extra-gastrointestinal disorders and is a major risk factor for gastric carcinoma and MALT lymphoma. Currently, numerous antibiotic-based therapies are available; however, these therapies have numerous drawbacks, mainly due to increasing prevalence of antibiotic resistant strains. Thus, there is an urgent need to develop novel therapeutic agents against H. pylori infections. MATERIALS AND METHODS: In this study, the anti-H...
August 31, 2016: Helicobacter
Iris Bosschem, Bram Flahou, Jaco Bakker, Edwin Heuvelman, Jan A M Langermans, Ellen De Bruyne, Myrthe Joosten, Annemieke Smet, Richard Ducatelle, Freddy Haesebrouck
BACKGROUND: Helicobacter suis (H. suis) is the most prevalent gastric non-H. pylori Helicobacter species in humans. This bacterium mainly colonizes the stomach of pigs, but it has also been detected in the stomach of nonhuman primates. The aim of this study was to obtain better insights into potential differences between pig- and primate-associated H. suis strains in virulence and pathogenesis. MATERIALS AND METHODS: In vitro-isolated H. suis strains obtained from pigs, cynomolgus monkeys (Macaca fascicularis), and rhesus monkeys (Macaca mulatta) were used for intragastric inoculation of BALB/c mice and Mongolian gerbils...
August 24, 2016: Helicobacter
Meijuan Zou, Fang Wang, Aiqin Jiang, Anliang Xia, Siya Kong, Chun Gong, Mingxia Zhu, Xin Zhou, Jun Zhu, Wei Zhu, Wenfang Cheng
BACKGROUND: Helicobacter pylori infection is the main cause of chronic gastritis, peptic ulcer, and gastric cancer. Tip-α is a newly identified carcinogenic factor present in H. pylori. TRAF3 can activate NF-κB by both canonical and noncanonical signaling pathways. In this study, we found that the expression of TRAF3 and NF-κB was upregulated, while microRNA-3178 (miR-3178) was decreased in H. pylori-positive gastric tissues but not in H. pylori-negative tissues. MATERIALS AND METHODS: GES-1 cells were incubated with 12...
August 5, 2016: Helicobacter
Nicoletta Di Simone, Chiara Tersigni, Simona Cardaropoli, Francesco Franceschi, Fiorella Di Nicuolo, Roberta Castellani, Francesca Bugli, Chiara de Waure, Anna Franca Cavaliere, Antonio Gasbarrini, Maurizio Sanguinetti, Giovanni Scambia, Tullia Todros
BACKGROUND: Preeclampsia (PE) is a major cause of maternal and neonatal morbidity and mortality. Epidemiological association between Helicobacter pylori (Hp) infection and PE onset has been widely shown. The aim of this study was to analyze a possible correlation between Hp infection and the severity of clinical presentation of PE and to identify an immunologic mechanism triggered by Hp infection potentially contributing to the pathogenesis of PE. MATERIALS AND METHODS: Sera from 93 preeclamptic women and 87 healthy pregnant women were tested for Hp infection by immunoassay and for anti-CagA antibodies by Western blot assay...
August 3, 2016: Helicobacter
Lifang Zhou, Fuju Zhao, Binjie Hu, Yi Fang, Yingxin Miao, Yiqin Huang, Da'nian Ji, Jinghao Zhang, Lingli Xu, Yanmei Zhang, Zhijun Bao, Hu Zhao
No abstract text is available yet for this article.
October 2016: Helicobacter
Zeki Yildirim, Bulent Bozkurt, Duygu Ozol, Ferah Armutcu, Recep Akgedik, Harun Karamanli, Deniz Kizilirmak, Mustafa İkizek
BACKGROUND: Helicobacter pylori (H. pylori) infection triggers both local inflammation, usually in gastric mucosa, and chronic systemic inflammation. It is assumed that this local and systemic inflammation is caused by extracellular products excreted by H. pylori. The aim of this study was to investigate the possible association between H. pylori infection and a local inflammatory response in the airway by using exhaled breath condensate technique. MATERIALS AND METHODS: This study includes 41 H...
October 2016: Helicobacter
Hyun Jin Jo, Jaeyeon Kim, Nayoung Kim, Ji Hyun Park, Ryoung Hee Nam, Yeong-Jae Seok, Yeon-Ran Kim, Joo Sung Kim, Jung Mogg Kim, Jung Min Kim, Dong Ho Lee, Hyun Chae Jung
BACKGROUND: Little is known about the role of gastric microbiota except for Helicobacter pylori (HP) in human health and disease. We compared the differences of human gastric microbiota according to gastric cancer or control and HP infection status and assessed the role of bacteria other than HP. METHODS: Gastric microbiota of 63 antral mucosal and 18 corpus mucosal samples were analyzed by bar-coded 454 pyrosequencing of the 16S rRNA gene. Antral samples were divided into four subgroups based on HP positivity in pyrosequencing and the presence of cancer...
October 2016: Helicobacter
Juan-Yu Piao, Hee Geum Lee, Su-Jung Kim, Do-Hee Kim, Hyeong-Jun Han, Hoang-Kieu-Chi Ngo, Sin-Aye Park, Jeong-Hwa Woo, Jeong-Sang Lee, Hye-Kyung Na, Young-Nam Cha, Young-Joon Surh
BACKGROUND: Recent studies have shown that Helicobacter pylori (H. pylori) activates signal transducer and activator of transcription 3 (STAT3) that plays an important role in gastric carcinogenesis. However, the molecular mechanism underlying H. pylori-mediated STAT3 activation is still not fully understood. In this study, we investigated H. pylori-induced activation of STAT3 signaling in AGS human gastric cancer cells and the underlying mechanism. MATERIALS AND METHODS: AGS cells were cocultured with H...
October 2016: Helicobacter
Cong He, Zhen Yang, Nonghua Lu
The development of new nucleotide sequencing techniques and advanced bioinformatics tools has opened the field for studying the diversity and complexity of the gastrointestinal microbiome independent of traditional cultural methods. Owing largely to the gastric acid barrier, the human stomach was long thought to be sterile. The discovery of Helicobacter pylori, the gram-negative bacterium that infects upwards of 50% of the global population, has started a major paradigm shift in our understanding of the stomach as an ecologic niche for bacteria...
October 2016: Helicobacter
Yue Hu, Meng Zhang, Bin Lu, Jinfeng Dai
Helicobacter pylori, a human pathogen with a high global prevalence, is the causative pathogen for multiple gastrointestinal diseases, especially chronic gastritis, peptic ulcers, gastric mucosa-associated lymphoid tissue lymphoma, and gastric malignancies. Antibiotic therapies remain the mainstay for H. pylori eradication; however, this strategy is hampered by the emergence and spread of H. pylori antibiotic resistance. Exploring the mechanistic basis of this resistance is becoming one of the major research questions in contemporary biomedical research, as such knowledge could be exploited to devise novel rational avenues for counteracting the existing resistance and devising strategies to avoid the development of a novel anti-H...
October 2016: Helicobacter
Aditi Banerjee, Malini Basu, Thomas G Blanchard, Subba R Chintalacharuvu, Wei Guang, Erik P Lillehoj, Steven J Czinn
BACKGROUND: Murine models of Helicobacter pylori infection are used to study host-pathogen interactions, but lack of severe gastritis in this model has limited its usefulness in studying pathogenesis. We compared the murine gastric epithelial cell line GSM06 to the human gastric epithelial AGS cell line to determine whether similar events occur when cultured with H. pylori. MATERIALS AND METHODS: The lysates of cells infected with H. pylori isolates or an isogenic cagA-deficient mutant were assessed for translocation and phosphorylation of CagA and for activation of stress pathway kinases by immunoblot...
October 2016: Helicobacter
Zhiqiang Song, Liya Zhou, Jianzhong Zhang, Lihua He, Peng Bai, Yan Xue
BACKGROUND: Hybrid therapy has recently attracted widespread attention. However, many issues require further exploration. For example, research in regions with high antibiotic resistance rates is limited, and the correlation between eradication efficacy and antibiotic resistance remains unclear. The aim of this study was to determine the efficacy, compliance, safety, and risk factors of hybrid therapy as first-line regimen in a region with high antibiotic resistance rates. MATERIALS AND METHODS: This prospective study was conducted in a tertiary hospital between January 2014 and June 2015...
October 2016: Helicobacter
Antonio Francesco Ciccaglione, Roberta Tavani, Laurino Grossi, Luigina Cellini, Lamberto Manzoli, Leonardo Marzio
AIM: To evaluate the therapeutic gain of the addition of bismuth to a rifabutin containing triple therapy with amoxicillin and pantoprazole at standard dosages for the treatment of third-line Helicobacter pylori infection after a preliminary susceptibility test. METHODS: Two separate groups of patients in two pilot studies which were carried out simultaneously. One group was treated with rifabutin 150 mg b.i.d., pantoprazole 20 mg b.i.d., and amoxicillin 1 g b.i...
October 2016: Helicobacter
Asif Sukri, Alfizah Hanafiah, Nik Ritza Kosai, Mustafa Mohamed Taher, Isa Mohamed Rose
BACKGROUND: Comprehensive immunophenotyping cluster of differentiation (CD) antigens in gastric adenocarcinoma, specifically between Helicobacter pylori-infected and -uninfected gastric cancer patients by using DotScan(™) antibody microarray has not been conducted. Current immunophenotyping techniques include flow cytometry and immunohistochemistry are limited to the use of few antibodies for parallel examination. We used DotScan(™) antibody microarray consisting 144 CD antibodies to determine the distribution of CD antigens in gastric adenocarcinoma cells and to elucidate the effect of H...
October 2016: Helicobacter
Yi Hu, Jian-Ping Liu, Yin Zhu, Nong-Hua Lu
Helicobacter pylori (H. pylori) is a common pathogenic bacterium in the stomach that infects almost half of the population worldwide and is closely related to gastric diseases and some extragastric diseases, including iron-deficiency anemia and idiopathic thrombocytopenic purpura. Both the Maastricht IV/Florence consensus report and the Kyoto global consensus report have proposed the eradication of H. pylori to prevent gastric cancer as H.pylori has been shown to be a major cause of gastric carcinogenesis. The interactions between H...
October 2016: Helicobacter
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