Chuang-Hsin Chiu, David J Yang, Yi-Chen Liou, Wei-Chung Chang, Tsung-Hsun Yu, Min-Ching Chung, Yen-Chun Lee, Ing-Jou Chen, Pao-Yeh Wang, Ching-Po Lin, Hey-Jen Tsay, Skye Hsin-Hsien Yeh
Background: DNA biomarkers are useful for the assessment of tumor cell proliferation. The authors aimed to synthesize a thiopurine-based ligand for evaluation of nuclear uptake and tumor localization. Materials and Methods: A 2-hydroxypropyl spacer was incorporated between a chelator (cyclam) and thiopurine ligand to produce SC-06-L1. In vitro cellular uptake and the cell/media ratios of [99m Tc]Tc-SC-06-L1 were assessed in breast (MCF-7, MDA-MB-231) and ovarian (TOV-112D, OVCAR3) cancer cells. The nuclear and cytosolic uptake ratio of [99m Tc]Tc-SC-06-L1 was determined in OVCAR-3 and MCF-7 cells...
March 8, 2024: Cancer Biotherapy & Radiopharmaceuticals