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Molecular Vision

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https://www.readbyqxmd.com/read/29769799/placental-growth-factor-promotes-epithelial-mesenchymal-transition-like-changes-in-arpe-19-cells-under-hypoxia
#1
Yi Zhang, Lin Zhao, Lijun Wang, Xiting Yang, Aiyi Zhou, Jianming Wang
Purpose: To investigate the role of placental growth factor (PGF) in the epithelial-mesenchymal transition (EMT) of ARPE-19 cells under hypoxia, and whether the NF-κB signaling pathway is involved in this process. Methods: ARPE-19 cells were treated in five groups: a control group, hypoxia group, PGF group, hypoxia+PGF group, and NF-κB-blocked group. A chemical hypoxia model was established in the ARPE-19 cells by adding CoCl2 to the culture medium. The morphological changes after treatment were observed...
2018: Molecular Vision
https://www.readbyqxmd.com/read/29769798/novel-variants-identified-with-next-generation-sequencing-in-polish-patients-with-cone-rod-dystrophy
#2
Anna Wawrocka, Anna Skorczyk-Werner, Katarzyna Wicher, Zuzanna Niedziela, Rafal Ploski, Malgorzata Rydzanicz, Maciej Sykulski, Jaroslaw Kociecki, Nicole Weisschuh, Susanne Kohl, Saskia Biskup, Bernd Wissinger, Maciej R Krawczynski
Purpose: The aim of this study was to identify the molecular genetic basis of cone-rod dystrophy in 18 unrelated families of Polish origin. Cone-rod dystrophy is one of the inherited retinal dystrophies, which constitute a highly heterogeneous group of disorders characterized by progressive dysfunction of photoreceptors and retinal pigment epithelium (RPE) cells. Methods: The study group was composed of four groups of patients representing different Mendelian inheritance of the disease: autosomal dominant (AD), autosomal recessive (AR), X-linked recessive (XL), and autosomal recessive or X-linked recessive (AR/XL)...
2018: Molecular Vision
https://www.readbyqxmd.com/read/29769797/protective-effects-of-erigeron-breviscapus-hand-mazz-ebhm-extract-in-retinal-neurodegeneration-models
#3
Jingyuan Zhu, Li Chen, Yun Qi, Jing Feng, Li Zhu, Yujing Bai, Huijuan Wu
Purpose: To investigate the neuroprotective effects of scutellarin, an active component of the multifunctional traditional Chinese herb Erigeron breviscapus (vant.) Hand.-Mazz. (EBHM), which has been used as a neuroprotective therapy for cerebrovascular diseases. We performed the experiments using in vitro and in vivo models of retinal neurodegeneration. Methods: In the in vitro experiments, we exposed BV-2 cells to low oxygen levels in an incubator for 24 and 48 h to generate hypoxia models...
2018: Molecular Vision
https://www.readbyqxmd.com/read/29706763/%C3%AE-a-crystallin-derived-minichaperone-stabilizes-%C3%AE-ag98r-crystallin-by-affecting-its-zeta-potential
#4
Ashutosh S Phadte, Puttur Santhoshkumar, K Krishna Sharma
Purpose: The G98R mutant of αA-crystallin is associated with the development of presenile cataracts. In vitro, the recombinant mutant protein exhibits altered structural and functional characteristics, along with the propensity to aggregate by itself and precipitate. Previously, we have reported that the N-terminal aspartate substituted form of the antiaggregation peptide, D71 FVIFLDVKHFSPEDLTVK88 (αA-minichaperone or mini-αA) prevented aggregation of αAG98R. However, the mechanism of stabilization of αAG98R from aggregation is not fully understood...
2018: Molecular Vision
https://www.readbyqxmd.com/read/29692599/structure-and-microanalysis-of-tear-film-ferning-of-camel-tears-human-tears-and-refresh-plus
#5
Masmali Am, Fagehi Ra, Ahmad H El-Naggar, Almubrad Tm
Purpose: This study aimed to investigate the tear ferning pattern and chemical elements of the tear film of camel tears compared with human tears and Refresh Plus eye lubricant. Refresh Plus was used as a control because it provides a healthy ferning pattern, due to the presence of an optimum ratio of carboxymethylcellulose (CMC) sodium and electrolytes. The main research focus is elucidating the viability of camel tear film in the dry, harsh environment of the desert. Methods: The tears were collected from five camels, five male desert workers (20-25 years old) at a small village located 100 km from Riyadh, Saudi Arabia, and five male subjects (20-25 years old) from Riyadh...
2018: Molecular Vision
https://www.readbyqxmd.com/read/29681726/early-onset-flecked-retinal-dystrophy-associated-with-new-compound-heterozygous-rpe65-variants
#6
Satoshi Katagiri, Katsuhiro Hosono, Takaaki Hayashi, Kentaro Kurata, Kei Mizobuchi, Tomokazu Matsuura, Kazutoshi Yoshitake, Takeshi Iwata, Tadashi Nakano, Yoshihiro Hotta
Purpose: To report genetic and clinical features of two unrelated Japanese patients with early onset flecked retinal dystrophy. Methods: Patients underwent comprehensive ophthalmic examinations that included electroretinography (ERG) after 30 min and 24 h of dark adaptation (DA). Disease-causing gene variants were identified with whole exome sequencing (WES), with identified candidates confirmed with direct sequencing. Results: WES identified compound heterozygous RPE65 variants in both patients...
2018: Molecular Vision
https://www.readbyqxmd.com/read/29681725/the-effects-of-bevacizumab-treatment-in-a-rat-model-of-retinal-ischemia-and-perfusion-injury
#7
Maryo Cenk Kohen, Sinan Tatlipinar, Alev Cumbul, Ünal Uslu
Purpose: To create a model of an ischemic retina with temporary ischemia and reperfusion (IR) and to examine the possible antiapoptotic and neurodegenerative effects of a vascular endothelial growth factor (VEGF) antagonist. Methods: Three groups were formed. Rats were subjected to continued ischemia for 45 min, and then reperfusion was allowed for 2 days. For the first group, ischemia was induced, but an anti-VEGF agent was not administered. For the second group, 2 days before ischemia, 0...
2018: Molecular Vision
https://www.readbyqxmd.com/read/29643724/resolvin-d1-promotes-corneal-epithelial-wound-healing-and-restoration-of-mechanical-sensation-in-diabetic-mice
#8
Zhenzhen Zhang, Xiaoli Hu, Xia Qi, Guohu Di, Yangyang Zhang, Qian Wang, Qingjun Zhou
Purpose: To investigate the effect and mechanism of proresolving lipid mediator resolvin D1 (RvD1) on the corneal epithelium and the restoration of mechanical sensation in diabetic mice. Methods: Type 1 diabetes was induced in mice with intraperitoneal streptozocin injections. The healthy and diabetic mice underwent removal of the central corneal epithelium, and then 100 ng/ml RvD1 or its formyl peptide receptor 2 (FPR2) antagonist WRW4 was used to treat the diabetic mice...
2018: Molecular Vision
https://www.readbyqxmd.com/read/29618921/-pax6-molecular-analysis-and-genotype-phenotype-correlations-in-families-with-aniridia-from-australasia-and-southeast-asia
#9
Emmanuelle Souzeau, Adam K Rudkin, Andrew Dubowsky, Robert J Casson, James S Muecke, Erica Mancel, Mark Whiting, Richard A D Mills, Kathryn P Burdon, Jamie E Craig
Purpose: Aniridia is a congenital disorder caused by variants in the PAX6 gene. In this study, we assessed the involvement of PAX6 in patients with aniridia from Australasia and Southeast Asia. Methods: Twenty-nine individuals with aniridia from 18 families originating from Australia, New Caledonia, Cambodia, Sri Lanka, and Bhutan were included. The PAX6 gene was investigated for sequence variants and analyzed for deletions with multiplex ligation-dependent probe amplification...
2018: Molecular Vision
https://www.readbyqxmd.com/read/29618920/vitreous-levels-of-apolipoprotein-a1-and-retinol-binding-protein-4-in-human-rhegmatogenous-retinal-detachment-associated-with-choroidal-detachment
#10
Nannan Ding, Shasha Luo, Jinjin Yu, Ying Zhou, Zhifeng Wu
Purpose: This study aims to quantify the concentration of apolipoprotein A1 (APOA1) and retinol binding protein (RBP4) expressed in the vitreous humors of patients with rhegmatogenous retinal detachment associated with choroidal detachment (RRDCD), rhegmatogenous retinal detachment (RRD), and idiopathic epimacular membrane (IEM). This study also aims to investigate the potential role of APOA1 and RBP4 as biomarkers of RRDCD. Methods: Enzyme-linked immunosorbent assay (ELISA) kits were used to obtain levels of APOA1 and RBP4 from the vitreous humor samples of 76 primary patients...
2018: Molecular Vision
https://www.readbyqxmd.com/read/29568217/mutational-screening-of-germline-rb1-gene-in-vietnamese-patients-with-retinoblastoma-reveals-three-novel-mutations
#11
Ha Hai Nguyen, Hoa Thi Thanh Nguyen, Nhung Phuong Vu, Quynh Thuy Le, Chau Minh Pham, Thuong Thi Huyen, Hung Manh, Hang Le Bich Pham, Ton Dang Nguyen, Hien Thi Thu Le, Hai Van Nong
Purpose: Retinoblastoma (Rb) is a rare and unique eye cancer that usually develops in the retinas of children less than 5 years old due to mutations in the RB1 gene. About 40% of affected individuals have the heritable form making genetics testing of the RB1 gene important for disease management. This study aims to identify germline mutations in RB1 in a cohort of patients with Rb from northern Vietnam. Methods: Genomic DNA was extracted from peripheral blood of 34 patients with Rb (nine unilateral and 25 bilateral cases) and their available parents...
2018: Molecular Vision
https://www.readbyqxmd.com/read/29563767/in-silico-modeling-of-epigenetic-induced-changes-in-photoreceptor-cis-regulatory-elements
#12
Reafa A Hossain, Nicholas R Dunham, Raymond A Enke, Christopher E Berndsen
Purpose: DNA methylation is a well-characterized epigenetic repressor of mRNA transcription in many plant and vertebrate systems. However, the mechanism of this repression is not fully understood. The process of transcription is controlled by proteins that regulate recruitment and activity of RNA polymerase by binding to specific cis-regulatory sequences. Cone-rod homeobox (CRX) is a well-characterized mammalian transcription factor that controls photoreceptor cell-specific gene expression...
2018: Molecular Vision
https://www.readbyqxmd.com/read/29527116/obesity-induced-metabolic-disturbance-drives-oxidative-stress-and-complement-activation-in-the-retinal-environment
#13
Riccardo Natoli, Nilisha Fernando, Tess Dahlenburg, Haihan Jiao, Riemke Aggio-Bruce, Nigel L Barnett, Juan Manuel Chao de la Barca, Guillaume Tcherkez, Pascal Reynier, Johnny Fang, Joshua A Chu-Tan, Krisztina Valter, Jan Provis, Matt Rutar
Purpose: Systemic increases in reactive oxygen species, and their association with inflammation, have been proposed as an underlying mechanism linking obesity and age-related macular degeneration (AMD). Studies have found increased levels of oxidative stress biomarkers and inflammatory cytokines in obese individuals; however, the correlation between obesity and retinal inflammation has yet to be assessed. We used the leptin-deficient (ob/ob) mouse to further our understanding of the contribution of obesity to retinal oxidative stress and inflammation...
2018: Molecular Vision
https://www.readbyqxmd.com/read/29527115/tacrolimus-interacts-with-voriconazole-to-reduce-the-severity-of-fungal-keratitis-by-suppressing-ifn-related-inflammatory-responses-and-concomitant-fk506-and-voriconazole-treatment-suppresses-fungal-keratitis
#14
Jing Zhong, Lulu Peng, Bowen Wang, Henan Zhang, Saiqun Li, Ruhui Yang, Yuqing Deng, Haixiang Huang, Jin Yuan
Purpose: To investigate the expression and roles of type I and II interferons (IFNs) in fungal keratitis, as well as the therapeutic effects of tacrolimus (FK506) and voriconazole on this condition. Methods: The mRNA and protein expression levels of type I (IFN-α/β) and II (IFN-γ) IFNs, as well as of related downstream inflammatory cytokines (interleukin (IL)-1α, IL-6, IL-12, and IL-17), were detected in macrophages, neutrophils, lymphocytes, and corneal epithelial cells (A6(1) cells) stimulated with zymosan (10 mg/ml) for 8 or 24 h...
2018: Molecular Vision
https://www.readbyqxmd.com/read/29463955/optic-nerve-regeneration-in-the-mouse-is-a-complex-trait-modulated-by-genetic-background
#15
Jiaxing Wang, Ying Li, Rebecca King, Felix L Struebing, Eldon E Geisert
Purpose: The present study is designed to identify the influences of genetic background on optic nerve regeneration using the two parental strains (C57BL/6J and DBA/2J) and seven BXD recombinant inbred mouse strains. Methods: To study regeneration in the optic nerve, Pten was knocked down in the retinal ganglion cells using adenoassociated virus (AAV) delivery of shRNA, and a mild inflammatory response was induced with an intravitreal injection of zymosan with CPT-cAMP...
2018: Molecular Vision
https://www.readbyqxmd.com/read/29463954/association-of-plasma-osteopontin-with-diabetic-retinopathy-in-asians-with-type-2-diabetes
#16
Xiao Zhang, Wai Kitt Chee, Sylvia Liu, Subramaniam Tavintharan, Chee Fang Sum, Su Chi Lim, Neelam Kumari
Purpose: Osteopontin (OPN) is a proinflammatory cytokine with diverse functions. Increased levels of OPN in vitreous fluid have been reported in patients with diabetic retinopathy (DR); however, studies on circulating OPN levels in DR are limited. We aim to examine the association of plasma OPN levels with the presence and severity of DR in a multiethnic cohort with type 2 diabetes mellitus (type 2 diabetes) in Singapore. Methods: Plasma levels of OPN were measured using enzyme-linked immunosorbent assay...
2018: Molecular Vision
https://www.readbyqxmd.com/read/29463953/autosomal-dominant-retinitis-pigmentosa-rhodopsin-mutant-q344x-drives-specific-alterations-in-chromatin-complex-gene-transcription
#17
Katie L Bales, Lara Ianov, Andrew J Kennedy, J David Sweatt, Alecia K Gross
Purpose: Epigenetic and transcriptional mechanisms have been shown to contribute to long-lasting functional changes in adult neurons. The purpose of this study was to identify any such modifications in diseased retinal tissues from a mouse model of rhodopsin mutation-associated autosomal dominant retinitis pigmentosa (ADRP), Q344X, relative to age-matched wild-type (WT) controls. Methods: We performed RNA sequencing (RNA-seq) at poly(A) selected RNA to profile the transcriptional patterns in 3-week-old ADRP mouse model rhodopsin Q344X compared to WT controls...
2018: Molecular Vision
https://www.readbyqxmd.com/read/29430168/assessment-of-p16-expression-and-hpv-infection-in-adenoid-cystic-carcinoma-of-the-lacrimal-gland
#18
Yuchuan Wang, Yuan Tian, Jinyong Lin, Luxia Chen, Liming Wang, Peng Hao, Ruifang Han, Ming Ying, Xuan Li, Xin Tang
Purpose: Adenoid cystic carcinoma (ACC) in the lacrimal gland is a rare malignancy. P16 is encoded by the CDKN2A gene, which is recognized as a tumor suppressor due to its inactivation in many types of tumors. However, p16 overexpression is also linked to adverse tumor parameters. These contradictory observations have also been confirmed in ACCs in the salivary glands. Furthermore, evidence of human papilloma virus (HPV) infection is found in a proportion of ACCs in the salivary glands...
2018: Molecular Vision
https://www.readbyqxmd.com/read/29430167/the-genetic-dissection-of-myo7a-gene-expression-in-the-retinas-of-bxd-mice
#19
Ye Lu, Diana Zhou, Rebecca King, Shuang Zhu, Claire L Simpson, Byron C Jones, Wenbo Zhang, Eldon E Geisert, Lu Lu
Purpose: Usher syndrome (US) is characterized by a loss of vision due to retinitis pigmentosa (RP) and deafness. US has three clinical subtypes, but even within each subtype, the severity varies. Myosin VIIA, coded by Myo7a , has been identified as one of the causal genes of US. This study aims to identify pathways and other genes through which Myo7a interacts to affect the presentation of US symptoms. Methods: In this study, we used the retinal tissue of BXD recombinant inbred (RI) mice to examine the expression of Myo7a and perform genetic mapping...
2018: Molecular Vision
https://www.readbyqxmd.com/read/29422769/a-genome-wide-association-study-of-corneal-astigmatism-the-cream-consortium
#20
Rupal L Shah, Qing Li, Wanting Zhao, Milly S Tedja, J Willem L Tideman, Anthony P Khawaja, Qiao Fan, Seyhan Yazar, Katie M Williams, Virginie J M Verhoeven, Jing Xie, Ya Xing Wang, Moritz Hess, Stefan Nickels, Karl J Lackner, Olavi Pärssinen, Juho Wedenoja, Ginevra Biino, Maria Pina Concas, André Uitterlinden, Fernando Rivadeneira, Vincent W V Jaddoe, Pirro G Hysi, Xueling Sim, Nicholas Tan, Yih-Chung Tham, Sonoko Sensaki, Albert Hofman, Johannes R Vingerling, Jost B Jonas, Paul Mitchell, Christopher J Hammond, René Höhn, Paul N Baird, Tien-Yin Wong, Chinfsg-Yu Cheng, Yik Ying Teo, David A Mackey, Cathy Williams, Seang-Mei Saw, Caroline C W Klaver, Jeremy A Guggenheim, Joan E Bailey-Wilson
Purpose: To identify genes and genetic markers associated with corneal astigmatism. Methods: A meta-analysis of genome-wide association studies (GWASs) of corneal astigmatism undertaken for 14 European ancestry (n=22,250) and 8 Asian ancestry (n=9,120) cohorts was performed by the Consortium for Refractive Error and Myopia. Cases were defined as having >0.75 diopters of corneal astigmatism. Subsequent gene-based and gene-set analyses of the meta-analyzed results of European ancestry cohorts were performed using VEGAS2 and MAGMA software...
2018: Molecular Vision
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