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Molecular Vision

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https://www.readbyqxmd.com/read/28484310/a-dot-hybridization-assay-for-the-diagnosis-of-bacterial-keratitis
#1
Po-Chiung Fang, Chun-Chih Chien, Hun-Ju Yu, Ren-Wen Ho, Shin-Ling Tseng, Yu-Hsuan Lai, Ming-Tse Kuo
PURPOSE: To evaluate a bacterial dot hybridization (BDH) assay for the diagnosis of bacterial keratitis (BK). METHODS: Sixty-one qualified corneal scrapings from 61 patients with suspected microbial keratitis were collected consecutively and prospectively. Among the 61 patients, 16 cases were BK and 45 cases were non-BK, including fungal keratitis, viral keratitis, parasitic keratitis, and non-microbial keratitis. Molecular diagnosis of BK in these corneal scrapes was performed using the BDH assay with three universal bacterial probes (PB1, PB2, and PB3) and three genus-specific probes (Aci, Klb, and Psu) to detect Acinetobacter, Klebsiella, and Pseudomonas, respectively...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28484309/identification-of-seven-novel-znf469-mutations-in-keratoconus-patients-in-a-han-chinese-population
#2
Xiaoning Yu, Binbin Chen, Xin Zhang, Xingchao Shentu
PURPOSE: To test for the potential presence of novel mutations in the zinc finger protein (ZNF469) gene in patients with sporadic keratoconus (KC) from a Han Chinese population. METHODS: Fifty-three patients with primary KC, 30 patients with high myopia (HM), and 100 unrelated population-matched healthy controls without any ocular or systemic disorders, all of Han Chinese ethnicity, were recruited. Blood samples were donated, and genomic DNA was isolated from peripheral blood leukocytes...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28479848/inhibitory-effects-of-s100a4-gene-silencing-on-alkali-burn-induced-corneal-neovascularization-an-in-vivo-study
#3
Yu-Lin Wang, Gui-Ping Gao, Yu-Qin Wang, Ying Wu, Zhi-You Peng, Quan Zhou
OBJECTIVE: The purpose of this study is to explore the inhibitory effects of S100A4 gene silencing on alkali burn-induced corneal neovascularization (CNV) in rabbit models. METHODS: Sixty-five rabbits were used to establish alkali-induced CNV models. After the operation, rabbits were given daily antibiotic eye drops and an eye ointment to prevent infection. The models were assigned to either an S100A4 siRNA or an empty vector group. Thirty rabbits were selected as the normal control group...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28465659/comparison-of-fibrin-glue-and-vicryl-sutures-in-conjunctival-autografting-for-pterygium-surgery
#4
Xiu Wang, Yan Zhang, Lei Zhou, Ruihua Wei, Lijie Dong
PURPOSE: To compare clinical parameters and the tear levels of inflammatory cytokines between pterygium surgery using sutures or fibrin glue. METHODS: Fifty-six patients with primary pterygium were divided into the suture group and the glue group, in which the autograft was secured with 10-0 Vicryl sutures and fibrin glue, respectively. A questionnaire, slit-lamp examination, Schirmer test, and visual acuity test were performed in all participants. Real-time quantitative PCR (q-PCR) was used to analyze the expression of genes in pterygium and healthy conjunctival tissues...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28465658/impact-of-obesity-with-impaired-glucose-tolerance-on-retinal-degeneration-in-a-rat-model-of-metabolic-syndrome
#5
Kishore Kumar Godisela, Singareddy Sreenivasa Reddy, Chekkilla Uday Kumar, Natarajan Saravanan, Paduru Yadagiri Reddy, Monica M Jablonski, Radha Ayyagari, Geereddy Bhanuprakash Reddy
PURPOSE: Metabolic syndrome (MetS) is associated with several degenerative diseases, including retinal degeneration. Previously, we reported on progressive retinal degeneration in a spontaneous obese rat (WNIN/Ob) model. In this study, we investigated the additional effect of impaired glucose tolerance (IGT), an essential component of MetS, on retinal degeneration using the WNIN/GR-Ob rat model. METHODS: The retinal morphology and ultrastructure of WNIN/GR-Ob and age-matched littermate lean rats were studied by microscopy and immunohistochemistry...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28465657/the-microrna-21-signaling-pathway-is-involved-in-prorenin-receptor-prr-induced-vegf-expression-in-arpe-19-cells-under-a-hyperglycemic-condition
#6
Rashidul Haque, P Michael Iuvone, Li He, Kimberly Su Chung Choi, Ashley Ngo, Samantha Gokhale, Madiha Aseem, Daniel Park
PURPOSE: MicroRNAs (miRNAs/miRs) are involved in a large number of biological functions and diseases, such as cancer, cardiovascular diseases, and diabetes. MiR-21 has been reported to target Sprouty homolog 1 (SPRY1), SMAD7, and PTEN. In this study, we examined the underlying role of miR-21 in the regulation of prorenin receptor (PRR)-mediated induction of vascular endothelial growth factor (VEGF) expression via targeting SMAD7, SPRY1, and PTEN in a hyperglycemic condition. METHODS: PRR-mediated induction of VEGF under a hyperglycemic condition (high glucose, 33mM) was studied by treating ARPE-19 cells with perindopril (10 µmol/l), which inhibits angiotensin II-mediated signaling...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28465656/resolvin-d1-inhibits-inflammatory-response-in-stz-induced-diabetic-retinopathy-rats-possible-involvement-of-nlrp3-inflammasome-and-nf-%C3%AE%C2%BAb-signaling-pathway
#7
Yizhou Yin, Fei Chen, Wenyan Wang, Han Wang, Xuedong Zhang
PURPOSE: To investigate the effect of resolvin D1 (RvD1) on the Nod-like receptor family pyrin domain-containing (NLRP3) inflammasome and the nuclear factor-kappa beta (NF-κB) pathway in streptozotocin (STZ)-induced diabetic retinopathy in rats. METHODS: Ninety-six male rats were divided into four groups: control, STZ, RvD1, and vehicle. The rats with diabetic retinopathy induced by STZ in the RvD1 and vehicle groups were given an intravitreal injection of RvD1 (1,000 ng/kg) or the same dosage of vehicle, respectively...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28465655/early-amd-like-defects-in-the-rpe-and-retinal-degeneration-in-aged-mice-with-rpe-specific-deletion-of-atg5-or-atg7
#8
Youwen Zhang, Samuel D Cross, James B Stanton, Alan D Marmorstein, Yun Zheng Le, Lihua Y Marmorstein
PURPOSE: To examine the effects of autophagy deficiency induced by RPE-specific deletion of Atg5 or Atg7 in mice as a function of age. METHODS: Conditional knockout mice with a floxed allele of Atg5 or Atg7 were crossed with inducible VMD2-rtTA/Cre transgenic mice. VMD2-directed RPE-specific Cre recombinase expression was induced with doxycycline feeding in the resulting mice. Cre-mediated deletion of floxed Atg5 or Atg7 resulted in RPE-specific inactivation of the Atg5 or Atg7 gene...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28458506/intraperitoneal-injection-of-epigallocatechin-3-gallate-protects-against-light-induced-photoreceptor-degeneration-in-the-mouse-retina
#9
Shounan Qi, Chenguang Wang, Delu Song, Ying Song, Joshua L Dunaief
PURPOSE: (-)-epigallocatechin-3-gallate (EGCG), a major catechin component of green tea, is reported to delay or prevent certain forms of cancer, arthritis, cardiovascular disease, and neurodegenerative disorders. In this study, we determined if systemically administered EGCG could protect the retina against light damage (LD) in mice. METHODS: BALB/cJ mice were treated with either EGCG or saline via intraperitoneal (IP) injection, and then placed under constant cool white light-emitting diode (LED) light (10,000 lux) for 5 h...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28458505/knock-in-of-cx46-partially-rescues-fiber-defects-in-lenses-lacking-cx50
#10
Eddie Wang, Andrew Geng, Richard Seo, Ankur Maniar, Xiaohua Gong
PURPOSE: Connexins 46 (Cx46) and 50 (Cx50) support lens development and homeostasis. Knockout (KO) of Cx50, but not Cx46, causes defects in lens fiber organization, F-actin enrichment, gap junction (GJ) size, ball-and-socket (BS) maturation, and GJ-associated protein distributions. To further determine the unique roles of Cx50 and Cx46, we investigated whether these defects persisted in Cx46 knock-in (Ki) lenses. Ki mice had Cx46 knocked-in to their Cx50 loci, where it was expressed under endogenous Cx50 promoters...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28446860/uvb-promotes-the-initiation-of-uveitic-inflammatory-injury-in-vivo-and-is-attenuated-by-uv-blocking-protection
#11
Yi-Ching Shao, Jyh-Cheng Liou, Chan-Yen Kuo, Yun-Shan Tsai, En-Chieh Lin, Ching-Ju Hsieh, Si-Ping Lin, Bo-Yie Chen
PURPOSE: Uveitic inflammatory injury can cause irreversible visual loss; however, no single animal model recapitulates all the characteristics of human uveitis. Ultraviolet radiation (UVR) is one of the risk factors for uveitis, but the role of UVR in the pathogenesis of uveitic injury is unclear. The aim of this study was to elucidate whether UVB promotes the initiation of, and subsequently contributes to, uveitic inflammatory injury. METHODS: Mice were assigned to either a blank control group or one of three UVB treatment groups: no protection, protection with Nelfilcon A contact lens (Food and Drug Administration [FDA] class II, about 46...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28442885/complement-c5a-receptor-knockout-has-diminished-light-induced-microglia-macrophage-retinal-migration
#12
Delu Song, Michael E Sulewski, Chenguang Wang, Jiantao Song, Rupak Bhuyan, Jacob Sterling, Esther Clark, Wen-Chao Song, Joshua L Dunaief
PURPOSE: The complement system is involved in the pathogenesis of age-related macular degeneration (AMD). Because activated microglia are also associated with AMD, we studied the relationship between complement anaphylatoxin receptors and microglial recruitment. METHODS: We assessed the effect of anaphylatoxin C3a receptor (C3aR) and C5a receptor (C5aR) knockout (KO) on light damage-induced migration of microglia/macrophages into the mouse outer retina via immunofluorescence and real-time quantitative PCR...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28442884/cone-dystrophy-or-macular-dystrophy-associated-with-novel-autosomal-dominant-guca1a-mutations
#13
Gaël Manes, Sonia Mamouni, Emilie Hérald, Anne-Claire Richard, Audrey Sénéchal, Karim Aouad, Béatrice Bocquet, Isabelle Meunier, Christian P Hamel
PURPOSE: Sixteen different mutations in the guanylate cyclase activator 1A gene (GUCA1A), have been previously identified to cause autosomal dominant cone dystrophy (adCOD), cone-rod dystrophy (adCORD), macular dystrophy (adMD), and in an isolated patient, retinitis pigmentosa (RP). The purpose of this study is to report on two novel mutations and the patients' clinical features. METHODS: Clinical investigations included visual acuity and visual field testing, fundus examination, high-resolution spectral-domain optical coherence tomography (OCT), fundus autofluorescence imaging, and full-field and multifocal electroretinogram (ERG) recordings...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28400700/serum-retinol-binding-protein-induced-endothelial-inflammation-is-mediated-through-the-activation-of-toll-like-receptor-4
#14
Mei Du, Ashley Martin, Franklin Hays, Jennifer Johnson, Rafal A Farjo, Krysten M Farjo
PURPOSE: Elevation of serum retinol-binding protein 4 (RBP4) induces inflammation in primary human retinal microvascular endothelial cells (HRECs) via a retinol-independent mechanism; thus, it may play a causative role in the development and progression of vascular lesions in diabetic retinopathy (DR). Since HRECs do not express the classical RBP4 receptor, stimulated by retinoic acid gene 6 (STRA6), this study focuses on identifying the endothelial cell receptor and signaling that mediate RBP4-induced inflammation...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28400699/ladd-syndrome-with-glaucoma-is-caused-by-a-novel-gene
#15
Allie Simpson, Armin Avdic, Ben R Roos, Adam DeLuca, Kathy Miller, Michael J Schnieders, Todd E Scheetz, Wallace L M Alward, John H Fingert
PURPOSE: Lacrimo-auriculo-dento-digital (LADD) syndrome is an autosomal dominant disorder displaying variable expression of multiple congenital anomalies including hypoplasia or aplasia of the lacrimal and salivary systems causing abnormal tearing and dry mouth. Mutations in the FGF10, FGFR2, and FGFR3 genes were found to cause some cases of LADD syndrome in prior genetic studies. The goal of this study is to identify the genetic basis of a case of LADD syndrome with glaucoma and thin central corneal thickness (CCT)...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28356707/epigenetic-intervention-with-a-bet-inhibitor-ameliorates-acute-retinal-ganglion-cell-death-in-mice
#16
Jun Li, Lei Zhao, Go Urabe, Yingmei Fu, Lian-Wang Guo
PURPOSE: The bromo and extraterminal (BET) epigenetic "reader" family is becoming an appealing new therapeutic target for several common diseases, yet little is known of its role in retinal neurodegeneration. We explored the potential of BET inhibition in the protection of retinal ganglion cells (RGCs). METHODS: To test the therapeutic effect of JQ1, an inhibitor highly selective for the BET family of proteins, we used an acute RGC damage model induced by N-methyl-D-aspartic acid (NMDA) excitotoxicity...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28356706/a-mutagenesis-derived-lrp5-mouse-mutant-with-abnormal-retinal-vasculature-and-low-bone-mineral-density
#17
Jeremy R Charette, Sarah E Earp, Brent A Bell, Cheryl L Ackert-Bicknell, Dana A Godfrey, Sujata Rao, Bela Anand-Apte, Patsy M Nishina, Neal S Peachey
PURPOSE: Familial exudative vitreoretinopathy (FEVR) is caused by mutations in the genes encoding low-density lipoprotein receptor-related protein (LRP5) or its interacting partners, namely frizzled class receptor 4 (FZD4) and norrin cystine knot growth factor (NDP). Mouse models for Lrp5, Fzd4, and Ndp have proven to be important for understanding the retinal pathophysiology underlying FEVR and systemic abnormalities related to defective Wnt signaling. Here, we report a new mouse mutant, tvrm111B, which was identified by electroretinogram (ERG) screening of mice generated in the Jackson Laboratory Translational Vision Research Models (TVRM) mutagenesis program...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28356705/novel-splice-site-mutation-in-ttll5-causes-cone-dystrophy-in-a-consanguineous-family
#18
Miguel de Sousa Dias, Christian P Hamel, Isabelle Meunier, Juliette Varin, Steven Blanchard, Fiona Boyard, José-Alain Sahel, Christina Zeitz
PURPOSE: To report the clinical and genetic findings of one family with autosomal recessive cone dystrophy (CD) and to identify the causative mutation. METHODS: An institutional study of three family members from two generations. The clinical examination included best-corrected Snellen visual acuity measurement, fundoscopy, the Farnsworth D-15 color vision test, a full-field electroretinogram (ERG) that incorporated the International Society for Clinical Electrophysiology of Vision standards and methodology, fundus autofluorescence (FAF) and infrared (IR), and spectral-domain optical coherence tomography (SD-OCT)...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28356704/osmotic-regulation-of-nfat5-expression-in-rpe-cells-the-involvement-of-purinergic-receptor-signaling
#19
Margrit Hollborn, Sarah Fischer, Heidrun Kuhrt, Peter Wiedemann, Andreas Bringmann, Leon Kohen
PURPOSE: Systemic hypertension is a risk factor for age-related neovascular retinal diseases. The major condition that induces hypertension is the intake of dietary salt (NaCl) resulting in increased extracellular osmolarity. High extracellular NaCl was has been shown to induce angiogenic factor production in RPE cells, in part via the transcriptional activity of nuclear factor of activated T cell 5 (NFAT5). Here, we determined the signaling pathways that mediate the osmotic expression of the NFAT5 gene in RPE cells...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28356703/differential-responses-of-choroidal-melanocytes-and-uveal-melanoma-cells-to-low-oxygen-conditions
#20
Cindy Weidmann, Jade Pomerleau, Laurence Trudel-Vandal, Solange Landreville
PURPOSE: Tissue culture is traditionally performed at atmospheric oxygen concentration (21%), which induces hyperoxic stress, as endogenous physiologic oxygen tension found in tissues varies between 2% and 9%. This discrepancy may lead to misinterpretation of results and may explain why effects observed in vitro cannot always be reproduced in vivo and vice versa. Only a few studies have been conducted in low physiologic oxygen conditions to understand the development and differentiation of cells from the eye...
2017: Molecular Vision
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