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Experimental Animals

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https://www.readbyqxmd.com/read/28413186/the-characterization-of-a-full-thickness-excision-open-foot-wound-model-in-n5-streptozotocin-stz-induced-type-2-diabetic-rats-that-mimics-diabetic-foot-ulcer-in-terms-of-reduced-blood-circulation-higher-c-reactive-protein-elevated-inflammation-and-reduced-cell
#1
Caroline Oi-Ling Yu, Kwok-Sui Leung, Kwok-Pui Fung, Francis Fu-Yuen Lam, Ethel Sau-Kuen Ng, Kit-Man Lau, Simon Kwoon-Ho Chow, Wing-Hoi Cheung
Delayed foot wound healing is a major complication attributed to hyperglycemia in type 2 diabetes mellitus (DM) patients, and these wounds may develop into foot ulcers. There are at least two types of DM wound models used in rodents to study delayed wound healing. However, clinically relevant animal models are not common. Most models use type 1 DM rodents or wounds created on the back rather than on the foot. An open full-thickness excision wound on the footpad of type 2 DM rats is more clinically relevant, but such a model has not yet been characterized systematically...
April 11, 2017: Experimental Animals
https://www.readbyqxmd.com/read/28381738/a-new-missense-mutation-in-the-paired-domain-of-the-mouse-pax3-gene
#2
Tamio Ohno, Tomoki Maegawa, Hiroto Katoh, Yuki Miyasaka, Miyako Suzuki, Misato Kobayashi, Fumihiko Horio
Mice with dominant white spotting occurred spontaneously in the C3.NSY-(D11Mit74-D11Mit229) strain. Linkage analysis indicated that the locus for white spotting was located in the vicinity of the Pax3 gene on chromosome 1. Crosses of white-spotted mice showed that homozygosity for the mutation caused tail and limb abnormalities and embryonic lethality as a result of exencephaly; these phenotypes were analogous to those found in other Pax3 mutants. Sequence analysis identified a missense point mutation (c.101G>A) in exon 2 of Pax3 that resulted in a methionine to isoleucine conversion at amino acid 62 of the PAX3 protein...
April 6, 2017: Experimental Animals
https://www.readbyqxmd.com/read/28381818/dronedarone-attenuates-the-duration-of-atrial-fibrillation-in-a-dog-model-of-sustained-atrial-fibrillation
#3
Nakkawee Saengklub, Vudhiporn Limprasutr, Suwanakiet Sawangkoon, Robert L Hamlin, Anusak Kijtawornrat
Atrial fibrillation (AF) is a supraventricular arrhythmia that leads to a decrease in cardiac output and impairs cardiac function and quality of life. Dronedarone has an atrial-selective property and has been used for management of AF in humans, but limited information is available in dogs. This study was designed to evaluate efficacy of dronedarone in attenuating the duration of AF in dog model of sustained AF. Six beagle dogs were anesthetized with isoflurane and instrumented to measure atrial action potential duration (aAPD) and atrial effective refractory period (AERP)...
April 4, 2017: Experimental Animals
https://www.readbyqxmd.com/read/28367864/body-surface-area-measurement-in-juvenile-miniature-pigs-using-a-computed-tomography-scanner
#4
Tadashi Itoh, Mifumi Kawabe, Takahiko Nagase, Hisami Matsushita, Masami Kato, Masafumi Miyoshi, Kazuro Miyahara
The use of miniature pigs in non-clinical studies for medical drugs or devices has gradually been increasing in recent years. It is anticipated that the use of juvenile miniature pigs in laboratory practice will also increase. Therefore, it is important to investigate various parameters of juvenile miniature pigs. The body surface area (BSA) of an organism is one of the important parameters for evaluating physiological functions. In drug development, normalization by BSA is an appropriate method for extrapolating doses between species...
March 30, 2017: Experimental Animals
https://www.readbyqxmd.com/read/28367863/ginsenoside-rb1-improves-cardiac-function-and-remodeling-in-heart-failure
#5
Xian Zheng, Shuai Wang, Xiaoming Zou, Yating Jing, Ronglai Yang, Siqi Li, Fengrong Wang
We investigated the effect of ginsenoside Rb1 on cardiac function and remodeling in heart failure (HF). Four weeks after HF induction, the rats were administrated with ginsenoside Rb1 (35 and 70 mg/kg) and losartan (4.5 mg/kg) for 8 weeks. Losartan was used as a positive control. Cardiac function was assessed by measuring hemodynamic parameters. Histological changes were analyzed by HE and Masson's trichrome staining. Cardiac hypertrophy, fibrosis, mitochondrial membrane potential and glucose transporter type 4 (GLUT4) levels were evaluated...
March 30, 2017: Experimental Animals
https://www.readbyqxmd.com/read/28367889/obesity-induction-in-hamster-that-mimics-the-human-clinical-condition
#6
Vivian Jordania DA Silva, Sílvia Regina Costa Dias, Tatiani Uceli Maioli, Luciana Ribeiro Serafim, Luis Fernando Viana Furtado, Maria da Gloria Quintão Silva, Ana Maria Caetano DE Faria, Élida Mara Leite Rabelo
Although obesity is well established in hamsters, studies using diets with high levels of simple carbohydrate associated with lipids are necessary to assess the impact of this type of food in the body. In this studya high sugar and butter diet (HSB) and high temperature were employed towards this end. Obesity was successfully induced at a temperature of 30.3ºC to 30.9°Cafter 38 days feeding the animals an HSB diet.It was shown that although diet is important for the induction of obesity, temperature is also essential because at a temperature slightly below the one required, obesity was not induced, even when the animals were fed for a longer period (150 days)...
March 28, 2017: Experimental Animals
https://www.readbyqxmd.com/read/28302963/potassium%C3%A2-oxonate-induces-acute-hyperuricemia-in-the-tree-shrew-tupaia-belangeri-chinensis
#7
Dong-Hong Tang, You-Song Ye, Chen-Yun Wang, Zhe-Li Li, Hong Zheng, Kai-Li Ma
Potassium oxonate, a selectively competitive uricase inhibitor, produced hyperuricemia (HUA) in rodents in a previous study. In this study, we employed the tree shrew as an animal model to study potassium oxonate-induced HUA. The effect of allopurinol (ALLO), a uric acid reducer, was also examined in this model. Potassium oxonate at doses of 5, 20, 40, 60, 80, 100, and 1000 mg/kg was given intraperitoneally to tree shrews. The results showed that potassium oxonate can effectively increase the levels of uric acid in tree shrews at doses ranging from 40 to 100 mg/kg...
March 16, 2017: Experimental Animals
https://www.readbyqxmd.com/read/28260717/difference-of-two-new-lcmv-strains-in-lethality-and-viral-genome-load-in-tissues
#8
Toshikazu Takagi, Makiko Ohsawa, Hitoki Yamanaka, Naoki Matsuda, Hiroshi Sato, Kazutaka Ohsawa
More than 30 strains of lymphocytic choriomeningitis virus (LCMV) have been isolated from mice, hamsters and humans in the United States, Europe and Japan. Experimentally infected mice exhibit different clinical signs and lethality depending on a combination of LCMV epitope peptides and host major histocompatibility complex (MHC) class I molecules. This study examined the pathogenicity, clinical signs and lethality, of two new LCMV strains (BRC and OQ28) using three inbred mouse strains with different genetic backgrounds having different H-2D haplotypes...
March 3, 2017: Experimental Animals
https://www.readbyqxmd.com/read/28228618/preventive-effect-of-intravesical-ozone-supplementation-on-n-methyl-n-nitrosourea-induced-non-muscle-invasive-bladder-cancer-in-male-rats
#9
Kerem Teke, Tayyar A Ozkan, Oguz O Cebeci, Hasan Yilmaz, Muhammed E Keles, Levend Ozkan, Meltem O Dillioglugil, Demir K Yildiz, Ozdal Dillioglugil
Although non-muscle invasive bladder cancer (NMIBC) is widely seen in men, most laboratory studies of new intravesical therapies to prevent NMIBC have been conducted on female animals. In addition, ozone (O3) has been shown to be a beneficial agent as an intravesical application in the treatment of various disorders. In the current study, we evaluated the immunohistopathological oxidative-antioxidative effects of intravesical O3 treatment on n-methyl-n-nitrosourea (MNU)-induced NMIBC. Male Wistar-Albino rats (n=51) were divided into four groups: sham (n=6), O3 only (n=15), MNU only (n=15), and MNU+O3 (n=15)...
February 22, 2017: Experimental Animals
https://www.readbyqxmd.com/read/28179596/nephron-wilms-tumor-1-wt1-and-synaptopodin-expression-in-developing-podocytes-of-mice
#10
Takashi Kato, Shinya Mizuno
Newborn mouse glomeruli are still immature with a morphological feature of an early capillary loop stage, but infant mice do not manifest proteinuria. Little is known about the molecular mechanism whereby infant mice are resistant to proteinuria. Nephrin and synaptopodin are crucial for slit diaphragm and foot process (FP) formation for avoiding proteinuria. Nephrin tyrosine phosphorylation means a transient biological signaling required for FP repair or extension during nephrotic disease. Using an immunohistochemical technique, we examined the natural course of nephrin, Wilms' tumor-1 (WT1) and synaptopodin at 16...
February 7, 2017: Experimental Animals
https://www.readbyqxmd.com/read/27784858/reproductive-experience-modified-dendritic-spines-on-cortical-pyramidal-neurons-to-enhance-sensory-perception-and-spatial-learning-in-rats
#11
Jeng-Rung Chen, Seh Hong Lim, Sin-Cun Chung, Yee-Fun Lee, Yueh-Jan Wang, Guo-Fang Tseng, Tsyr-Jiuan Wang
Behavioral adaptations during motherhood are aimed at increasing reproductive success. Alterations of hormones during motherhood could trigger brain morphological changes to underlie behavioral alterations. Here we investigated whether motherhood changes a rat's sensory perception and spatial memory in conjunction with cortical neuronal structural changes. Female rats of different statuses, including virgin, pregnant, lactating, and primiparous rats were studied. Behavioral test showed that the lactating rats were most sensitive to heat, while rats with motherhood and reproduction experience outperformed virgin rats in a water maze task...
January 27, 2017: Experimental Animals
https://www.readbyqxmd.com/read/27760894/development-of-reproductive-engineering-techniques-at-the-riken-bioresource-center
#12
REVIEW
Atsuo Ogura
Reproductive engineering techniques are essential for assisted reproduction of animals and generation of genetically modified animals. They may also provide invaluable research models for understanding the mechanisms involved in the developmental and reproductive processes. At the RIKEN BioResource Center (BRC), I have sought to develop new reproductive engineering techniques, especially those related to cryopreservation, microinsemination (sperm injection), nuclear transfer, and generation of new stem cell lines and animals, hoping that they will support the present and future projects at BRC...
January 27, 2017: Experimental Animals
https://www.readbyqxmd.com/read/27760892/investigation-of-sleep-wake-rhythm-in-non-human-primates-without-restraint-during-data-collection
#13
Akiyoshi Ishikawa, Keita Sakai, Takehiro Maki, Yuri Mizuno, Kimie Niimi, Yasuhiro Oda, Eiki Takahashi
To understand sleep mechanisms and develop treatments for sleep disorders, investigations using animal models are essential. The sleep architecture of rodents differs from that of diurnal mammals including humans and non-human primates. Sleep studies have been conducted in non-human primates; however, these sleep assessments were performed on animals placed in a restraint chair connected via the umbilical area to the recording apparatus. To avoid restraints, cables, and other stressful apparatuses and manipulations, telemetry systems have been developed...
January 27, 2017: Experimental Animals
https://www.readbyqxmd.com/read/27725374/rnai-mediated-knockdown-of-mouse-melanocortin-4-receptor-in-vitro-and-in-vivo-using-an-sirna-expression-construct-based-on-the-mir-187-precursor
#14
Minoru Kato, Yi-Ying Huang, Mina Matsuo, Yoko Takashina, Kazuyo Sasaki, Yasushi Horai, Aya Juni, Shin-Ichi Kamijo, Kaoru Saigo, Kumiko Ui-Tei, Hajime Tei
RNA interference (RNAi) is a powerful tool for the study of gene function in mammalian systems, including transgenic mice. Here, we report a gene knockdown system based on the human mir-187 precursor. We introduced small interfering RNA (siRNA) sequences against the mouse melanocortin-4 receptor (mMc4r) to alter the targeting of miR-187. The siRNA-expressing cassette was placed under the control of the cytomegalovirus (CMV) early enhancer/chicken β-actin promoter. In vitro, the construct efficiently knocked down the gene expression of a co-transfected mMc4r-expression vector in cultured mammalian cells...
January 27, 2017: Experimental Animals
https://www.readbyqxmd.com/read/27665862/dose-and-time-related-effects-of-caffeine-on-the-testis-in-immature-male-rats
#15
Jaeman Bae, Hyeonhae Choi, Yuri Choi, Jaesook Roh
We previously showed that prepubertal chronic caffeine exposure adversely affected the development of the testes in male rats. Here we investigated dose- and time-related effects of caffeine consumption on the testis throughout sexual maturation in prepubertal rats. A total of 80 male SD rats were randomly divided into four groups: controls and rats fed 20, 60, or 120 mg caffeine/kg/day, respectively, via gavage for 10, 20, 30, or 40 days. Preputial separation was monitored daily before the rats were sacrificed...
January 27, 2017: Experimental Animals
https://www.readbyqxmd.com/read/27523096/effects-of-agmatine-on-chlorpromazine-toxicity-in-the-liver-of-wistar-rats-the-possible-role-of-oxidant-antioxidant-imbalance
#16
Dejanovic Bratislav, Lavrnja Irena, Ninkovic Milica, Stojanovic Ivana, Djuric Ana, Dilber Sanda, Stevanovic Ivana
Chlorpromazine (CPZ) is a member of a widely used class of antipsychotic agents. The metabolic pathways of CPZ toxicity were examined by monitoring oxidative/nitrosative stress markers. The aim of the study was to investigate the hypothesis that agmatine (AGM) prevents oxidative stress in the liver of Wistar rats 48 h after administration of CPZ. All tested compounds were administered intraperitoneally (i.p.) in one single dose. The animals were divided into control (C, 0.9% saline solution), CPZ (CPZ, 38.7 mg/kg b...
January 27, 2017: Experimental Animals
https://www.readbyqxmd.com/read/28100889/cotransfer-of-regulatory-t-cells-improve-the-therapeutic-effectiveness-of-mesenchymal-stem-cells-in-treating-a-colitis-mouse-model
#17
Yang Yu, Tianyu Zhao, Deqin Yang
Inflammatory bowel disease (IBD) is a severe inflammatory condition in the colon. To date, clinical solutions for this disease have been limited. Mesenchymal stem cells (MSCs), multipotential cells with immune regulation and anti-inflammatory functions, have been applied to treatment of IBD. However, the therapeutic effectiveness of MSCs still needs to be improved. Here, we were interested in whether regulatory T cells (Tregs) could enhance the immune regulation function of MSCs in treatment of mouse colitis...
January 19, 2017: Experimental Animals
https://www.readbyqxmd.com/read/28100888/%C3%AE-hydroxybutyrate-levels-in-serum-and-cerebrospinal-fluid-under-ketone-body-metabolism-in-rats
#18
Xiaomeng Wang, Qi Liu, Jian Zhou, Xiuhua Wu, Qingan Zhu
A high-fat, low-carbohydrate diet (KD) or calorie restriction in the form of every-other-day fasting (EODF) results in ketone body metabolism with an increasing β-hydroxybutyrate (βOHB) level. Previous studies have supported that a KD and EODF have a neuroprotective effect. However, the βOHB levels in the cerebrospinal fluid (CSF) resulting from a KD and EODF remain unknown. The aim of this study was to detect βOHB levels in rats fed a KD, EODF diet, and every-other-day ketogenic diet (EODKD) and to compare the serum βOHB level with the CSF βOHB level...
January 19, 2017: Experimental Animals
https://www.readbyqxmd.com/read/28049885/murine-norovirus-infection-in-brazilian-animal-facilities
#19
Daniele Masselli Rodrigues, Josélia Cristina de Oliveira Moreira, Marcelo Lancellotti, Rovilson Gilioli, Marcus Alexandre Finzi Corat
Murine norovirus (MNV) is a single-stranded positive-sense RNA virus of the Caliciviridae family. MNV has been reported to infect laboratory mice with the ability to cause lethal infections in strains lacking components of the innate immune response. Currently, MNV is considered the most prevalent infectious agent detected in laboratory mouse facilities. In this study, mice in 22 laboratory animal facilities within Brazil were analyzed for MNV infection. Using primers targeting a conserved region of the viral capsid, MNV was detected by RT-PCR in 137 of 359 mice from all 22 facilities...
December 29, 2016: Experimental Animals
https://www.readbyqxmd.com/read/28025424/morphological-features-of-coronary-plaques-in-whhlmi-rabbits-oryctolagus-cuniculus-an-animal-model-for-familial-hypercholesterolemia
#20
Satoshi Yamada, Tomonari Koike, Takayuki Nakagawa, Nobue Kuniyoshi, Yu Ying, Hiroyuki Itabe, Atsushi Yamashita, Yuji Asada, Masashi Shiomi
In order to examine their suitability for studies on coronary atherosclerosis, we evaluated the features of coronary atherosclerotic plaques in myocardial infarction-prone Watanabe heritable hyperlipidemic (WHHLMI) rabbits, a spontaneous animal model for coronary atherosclerosis and myocardial infarction. Coronary segments of the hearts of 187 WHHLMI rabbits (10-29 months old) were sectioned serially and stained histopathologically and immunohistologically. Progression of coronary lesions was prominent in rabbits that had died suddenly...
December 27, 2016: Experimental Animals
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