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Nature Biotechnology

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https://www.readbyqxmd.com/read/29131149/three-dimensional-map-of-nonhematopoietic-bone-and-bone-marrow-cells-and-molecules
#1
Daniel L Coutu, Konstantinos D Kokkaliaris, Leo Kunz, Timm Schroeder
The bone marrow (BM) microenvironment contains many types of cells and molecules with roles in hematopoiesis, osteogenesis, angiogenesis and metabolism. The spatial distribution of the different bone and BM cell types remains elusive, owing to technical challenges associated with bone imaging. To map nonhematopoietic cells and structures in bone and BM, we performed multicolor 3D imaging of osteoblastic, vascular, perivascular, neuronal and marrow stromal cells, and extracellular-matrix proteins in whole mouse femurs...
November 13, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/29131148/structure-guided-chemical-modification-of-guide-rna-enables-potent-non-viral-in-vivo-genome-editing
#2
Hao Yin, Chun-Qing Song, Sneha Suresh, Qiongqiong Wu, Stephen Walsh, Luke Hyunsik Rhym, Esther Mintzer, Mehmet Fatih Bolukbasi, Lihua Julie Zhu, Kevin Kauffman, Haiwei Mou, Alicia Oberholzer, Junmei Ding, Suet-Yan Kwan, Roman L Bogorad, Timofei Zatsepin, Victor Koteliansky, Scot A Wolfe, Wen Xue, Robert Langer, Daniel G Anderson
Efficient genome editing with Cas9-sgRNA in vivo has required the use of viral delivery systems, which have limitations for clinical applications. Translational efforts to develop other RNA therapeutics have shown that judicious chemical modification of RNAs can improve therapeutic efficacy by reducing susceptibility to nuclease degradation. Guided by the structure of the Cas9-sgRNA complex, we identify regions of sgRNA that can be modified while maintaining or enhancing genome-editing activity, and we develop an optimal set of chemical modifications for in vivo applications...
November 13, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/29131147/accurate-assembly-of-transcripts-through-phase-preserving-graph-decomposition
#3
Mingfu Shao, Carl Kingsford
We introduce Scallop, an accurate reference-based transcript assembler that improves reconstruction of multi-exon and lowly expressed transcripts. Scallop preserves long-range phasing paths extracted from reads, while producing a parsimonious set of transcripts and minimizing coverage deviation. On 10 human RNA-seq samples, Scallop produces 34.5% and 36.3% more correct multi-exon transcripts than StringTie and TransComb, and respectively identifies 67.5% and 52.3% more lowly expressed transcripts. Scallop achieves higher sensitivity and precision than previous approaches over a wide range of coverage thresholds...
November 13, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/29121011/assisted-reproductive-technologies-to-prevent-human-mitochondrial-disease-transmission
#4
Andy Greenfield, Peter Braude, Frances Flinter, Robin Lovell-Badge, Caroline Ogilvie, Anthony C F Perry
Mitochondria are essential cytoplasmic organelles that generate energy (ATP) by oxidative phosphorylation and mediate key cellular processes such as apoptosis. They are maternally inherited and in humans contain a 16,569-base-pair circular genome (mtDNA) encoding 37 genes required for oxidative phosphorylation. Mutations in mtDNA cause a range of pathologies, commonly affecting energy-demanding tissues such as muscle and brain. Because mitochondrial diseases are incurable, attention has focused on limiting the inheritance of pathogenic mtDNA by mitochondrial replacement therapy (MRT)...
November 9, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/29106410/generation-of-higher-affinity-t-cell-receptors-by-antigen-driven-differentiation-of-progenitor-t-cells-in-vitro
#5
Thomas M Schmitt, David H Aggen, Kumiko Ishida-Tsubota, Sebastian Ochsenreither, David M Kranz, Philip D Greenberg
Many promising targets for T-cell-based cancer immunotherapies are self-antigens. During thymic selection, T cells bearing T cell receptors (TCRs) with high affinity for self-antigen are eliminated. The affinity of the remaining low-avidity TCRs can be improved to increase their antitumor efficacy, but conventional saturation mutagenesis approaches are labor intensive, and the resulting TCRs may be cross-reactive. Here we describe the in vitro maturation and selection of mouse and human T cells on antigen-expressing feeder cells to develop higher-affinity TCRs...
November 6, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/29106409/dna-sequencing-at-ultra-high-fidelity
#6
Robert Sebra
No abstract text is available yet for this article.
November 6, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/29106408/cell-type-specific-metabolic-labeling-of-nascent-proteomes-in-vivo
#7
Beatriz Alvarez-Castelao, Christoph T Schanzenbächer, Cyril Hanus, Caspar Glock, Susanne Tom Dieck, Aline R Dörrbaum, Ina Bartnik, Belquis Nassim-Assir, Elena Ciirdaeva, Anke Mueller, Daniela C Dieterich, David A Tirrell, Julian D Langer, Erin M Schuman
Although advances in protein labeling methods have made it possible to measure the proteome of mixed cell populations, it has not been possible to isolate cell-type-specific proteomes in vivo. This is because the existing methods for metabolic protein labeling in vivo access all cell types. We report the development of a transgenic mouse line where Cre-recombinase-induced expression of a mutant methionyl-tRNA synthetase (L274G) enables the cell-type-specific labeling of nascent proteins with a non-canonical amino-acid and click chemistry...
November 6, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/29106407/highly-accurate-fluorogenic-dna-sequencing-with-information-theory-based-error-correction
#8
Zitian Chen, Wenxiong Zhou, Shuo Qiao, Li Kang, Haifeng Duan, X Sunney Xie, Yanyi Huang
Eliminating errors in next-generation DNA sequencing has proved challenging. Here we present error-correction code (ECC) sequencing, a method to greatly improve sequencing accuracy by combining fluorogenic sequencing-by-synthesis (SBS) with an information theory-based error-correction algorithm. ECC embeds redundancy in sequencing reads by creating three orthogonal degenerate sequences, generated by alternate dual-base reactions. This is similar to encoding and decoding strategies that have proved effective in detecting and correcting errors in information communication and storage...
November 6, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/29106406/tuning-up-t-cell-receptors
#9
C Garrett Rappazzo, Michael E Birnbaum
No abstract text is available yet for this article.
November 6, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/29121031/better-beings
#10
Amber Dance
No abstract text is available yet for this article.
November 9, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/29121029/coiled-coils-unspring-protein-origami
#11
Greg L Hura, John A Tainer
No abstract text is available yet for this article.
November 9, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/29121028/with-alnylam-s-amyloidosis-success-rnai-approval-hopes-soar
#12
Cormac Sheridan
No abstract text is available yet for this article.
November 9, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/29121027/age-related-macular-degeneration-foils-drugmakers
#13
Elie Dolgin
No abstract text is available yet for this article.
November 9, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/29121026/humans-2-0
#14
EDITORIAL
(no author information available yet)
No abstract text is available yet for this article.
November 9, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/29121025/query-the-merits-of-embryo-editing-for-reproductive-research-now
#15
Insoo Hyun, Catherine Osborn
No abstract text is available yet for this article.
November 9, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/29121024/ethical-principles-for-the-use-of-human-cellular-biotechnologies
#16
Paul Root Wolpe, Karen S Rommelfanger
No abstract text is available yet for this article.
November 9, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/29121023/corrections
#17
(no author information available yet)
This corrects the article DOI: 10.1038/nbt0617-493.
November 9, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/29121022/public-views-on-gene-editing-and-its-uses
#18
George Gaskell, Imre Bard, Agnes Allansdottir, Rui Vieira da Cunha, Peter Eduard, Juergen Hampel, Elisabeth Hildt, Christian Hofmaier, Nicole Kronberger, Sheena Laursen, Anna Meijknecht, Salvör Nordal, Alexandre Quintanilha, Gema Revuelta, Núria Saladié, Judit Sándor, Júlio Borlido Santos, Simone Seyringer, Ilina Singh, Han Somsen, Winnie Toonders, Helge Torgersen, Vincent Torre, Márton Varju, Hub Zwart
No abstract text is available yet for this article.
November 9, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/29121021/revisiting-the-warnock-rule
#19
J Benjamin Hurlbut, Insoo Hyun, Aaron D Levine, Robin Lovell-Badge, Jeantine E Lunshof, Kirstin R W Matthews, Peter Mills, Alison Murdoch, Martin F Pera, Christopher Thomas Scott, Juliet Tizzard, Mary Warnock, Magdalena Zernicka-Goetz, Qi Zhou, Laurie Zoloth
No abstract text is available yet for this article.
November 9, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/29121020/first-rounders-stelios-papadopoulos
#20
(no author information available yet)
No abstract text is available yet for this article.
November 9, 2017: Nature Biotechnology
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