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New Microbiologica

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https://www.readbyqxmd.com/read/28675248/quick-bacteriophage-mediated-bioluminescence-assay-for-detecting-staphylococcus-spp-in-sonicate-fluid-of-orthopaedic-artificial-joints
#1
Katja Šuster, Aleš Podgornik, Andrej Cör
Staphylococcus spp. accounts for up to two thirds of all microorganisms causing prosthetic joint infections, with Staphylococcus aureus and Staphylococcus epidermidis being the major cause. The present study describes a diagnostic model to detect staphylococci using a specific bacteriophage and bioluminescence detection, exploring the possibility of its use on sonicate fluid of orthopaedic artificial joints. Intracellular adenosine-5'-triphosphate release by bacteriophage mediated lysis of staphylococci was assessed to determine optimal parameters for detection...
July 4, 2017: New Microbiologica
https://www.readbyqxmd.com/read/28675247/protection-by-humoral-elements-independent-of-virus-neutralization-activity-against-an-influenza-virus-challenge
#2
Julia Sarkadi, David Kuti, Mate Jankovics, Eva Pallinger, Kinga Fodor, Zoltan Kis, Eva Gonczol, Ildiko Visontai, Istvan Jankovics
To investigate the protective efficacy of a seasonal trivalent inactivated whole virion influenza vaccine (TIV) adjuvanted with aluminum phosphate (Fluval AB, referred to here as TIV+Al), we immunized mice with the TIV+Al, and as controls, with TIV, TIV+Al combined with Freund adjuvant (TIV+Al+F), inactivated A/PR/8/34(H1N1) (PR8) strain or PBS, and challenged them with a lethal dose of a mouse-adapted PR8 virus. Serum pools from immunized mice were passively transferred to recipient mice that were then challenged similarly...
July 4, 2017: New Microbiologica
https://www.readbyqxmd.com/read/28675246/clinical-and-in-vitro-efficacy-of-colistin-plus-vancomycin-and-rifampin-against-colistin-resistant-acinetobacter-baumannii-causing-ventilator-associated-pneumonia
#3
Alessandra Oliva, Alessia Cipolla, Vincenzo Vullo, Mario Venditti, Claudio Maria Mastroianni, Marco Falcone
We present the case of a patient with ventilator-associated pneumonia (VAP) caused by a pan-resistant Acinetobacter baumannii successfully treated with the combination colistin plus vancomycin plus rifampin, whose in vitro activity was investigated by checkerboard method and killing testing. Furthermore, the serum bactericidal activity (SBA) was assessed. Our case shows that an innovative regimen consisting of colistin plus antimicrobials active only against Gram-positive microorganisms might represent a valid therapeutic option for severe infections caused by colistin-resistant A...
July 4, 2017: New Microbiologica
https://www.readbyqxmd.com/read/28675245/plasmodium-knowlesi-malaria-in-a-traveller-returning-from-the-philippines-to-italy-2016
#4
Ettore De Canale, Dino Sgarabotto, Giulia Marini, Nicola Menegotto, Serena Masiero, Wassim Akkouche, Maria Angela Biasolo, Luisa Barzon, Giorgio Palù
Plasmodium knowlesi is a simian parasite responsible for most human cases of malaria in Malaysian Borneo. A timely recognition of infection is crucial because of the risk of severe disease due to the rapid increase in parasitemia. We report a case of P. knowlesi infection in a traveller who developed fever and thrombocytopenia after returning from the Philippines in 2016. Rapid antigen test was negative, microscopy examination showed parasites similar to Plasmodium malariae, with a parasite count of 10,000 parasites per μL blood, while molecular testing identified P...
July 4, 2017: New Microbiologica
https://www.readbyqxmd.com/read/28675244/a-catheter-related-bloodstream-infection-caused-by-chryseobacterium-indologenes-successfully-treated-with-antibiotic-lock-rescue-therapy
#5
Marta Corbella, Micaela Brandolini, Patrizia Cambieri, Nunzia Decembrino, Michele Pagani, Andrea Bottazzi, Alba Muzzi, Marco Zecca, Bianca Mariani, Piero Marone
We report the case of a catheter-related bloodstream infection caused by Chryseobacterium indologenes, an uncommon and multi-resistant pathogen, in a pediatric patient with a long-term vascular access device placed for chemotherapy treatment. The infection was successfully treated with ciprofloxacin antibiotic-lock therapy. This is the first report on successful salvage of a long-term device colonized by multi-resistant Chryseobacterium indologenes.
July 4, 2017: New Microbiologica
https://www.readbyqxmd.com/read/28675243/biotech-innovations-in-the-prevention-of-respiratory-infectious-diseases
#6
Julia Valeria Cento, Silvia Barbaliscia, Carlo Federico Perno
review is the creation of a biocompatible, biogel-based nasal filter enriched with AgNPs. Compared to traditional personal protective equipment (PPE), this type of nasal filter has the advantage of combining the antibacterial and antiviral activity of AgNPs with the common filtration capacity shared by other PPEs. This dual mechanism means that AgNP-enriched nasal filters serve to reduce the infecting microbial-load and protect the lower airways, without interfering with the normal respiratory capacity (airflow-resistance <5%)...
July 4, 2017: New Microbiologica
https://www.readbyqxmd.com/read/28675242/the-sigma-factor-sigd-of-mycobacterium-tuberculosis-putatively-enhances-gene-expression-of-the-septum-site-determining-protein-under-stressful-environments
#7
Miguel A Ares, Nora Rios-Sarabia, Miguel A De la Cruz, Sandra Rivera-Gutiérrez, Lázaro García-Morales, Lizbel León-Solís, Clara Espitia, Sabino Pacheco, Jorge F Cerna-Cortés, Cecilia A Helguera-Repetto, María Jesús García, Jorge A González-Y-Merchand
This work examined the expression of the septum site determining gene (ssd) of Mycobacterium tuberculosis CDC1551 and its ΔsigD mutant under different growing conditions. The results showed an up-regulation of ssd during stationary phase and starvation conditions, but not during in vitro dormancy, suggesting a putative role for SigD in the control of ssd expression mainly under lack-of-nutrients environments. Furthermore, we elucidated a putative link between ssd expression and cell elongation of bacilli at stationary phase...
July 4, 2017: New Microbiologica
https://www.readbyqxmd.com/read/28513815/epidemiology-of-paediatric-meningitis-in-central-c%C3%A3-te-d-ivoire-after-the-implementation-of-haemophilus-influenzae-type-b-vaccination
#8
Fidèle S Touré, Samson Kouame, Honoré Tia, Pacôme Monemo, Amadou Cissé, Bamourou Diané, Sören L Becker, Chantal Akoua-Koffi
Infectious meningitis accounts for enormous morbidity worldwide, but there is a paucity of data on its regional epidemiology in resource-constrained settings of sub-Saharan Africa. Here, we present a study on the aetiology of paediatric meningitis in central Côte d'Ivoire. Between June 2012 and December 2013, all cerebrospinal fluid (CSF) samples drawn at the University Teaching Hospital Bouaké were examined for the presence of bacterial and fungal pathogens. A causative agent was detected in 31 out of 833 CSF specimens (3...
May 17, 2017: New Microbiologica
https://www.readbyqxmd.com/read/28513814/zika-virus-isolation-from-semen
#9
Elena Percivalle, Maurizio Zavattoni, Fausto Fausto, Francesca Rovida
Zika virus (ZIKV) can be sexually transmitted and replicative particles were first detected in a semen sample from a patient during the 2013-14 French Polynesia outbreak. Here we describe the virus isolation from semen of a patient returning to Italy from Brazil.
May 17, 2017: New Microbiologica
https://www.readbyqxmd.com/read/28513813/hepatitis-b-virus-reactivation-after-effective-sofosbuvir-and-ribavirin-treatment-in-a-patient-with-occult-hepatitis-b-virus-infection
#10
Silvia Odolini, Paola Lanza, Angiola Spinetti, Serena Zaltron, Lucia Urbinati, Andrea Vavassori, Paola Nasta, Elena Festa, Franco Gargiulo, Anna Rodella, Arnaldo Caruso, Salvatore Casari, Francesco Castelli, Mauro Viganò
Reactivation of the hepatitis B virus (HBV) has been reported in patients with occult infection (OBI), i.e. HBV surface antigen (HBsAg) negative, HBV core antibody (anti-HBc) positive ± antibodies against HBsAg (anti-HBs) and detectable HBV DNA in serum or liver, receiving immunosuppressive or cytotoxic therapies. Recently, concerns have been raised regarding the risk of HBV reactivation in OBI patients treated with direct acting antiviral agents (DAAs) for chronic hepatitis C (CHC). Here we describe a case of HBV reactivation in a 72-year-old woman with OBI as a possible consequence of effective treatment with sofosbuvir (SOF) and ribavirin (Rbv) for genotype 2a/2c CHC...
May 17, 2017: New Microbiologica
https://www.readbyqxmd.com/read/28513812/common-dermatophytes-and-in-vitro-anti-fungal-susceptibility-testing-in-patients-attending-the-dermatological-clinic-at-the-hospital-for-tropical-medicine-bangkok
#11
Watcharamat Muangkaew, Thanwa Wongsuk, Natthanej Luplertlop
Dermatophytes comprising the genera Trichophyton, Microsporum, and Epidermophyton are important causes of superficial mycoses. The number of infected patients and the distribution of species of these organisms in our hospital were unknown. We therefore aimed to investigate the clinical pattern of dermatophyte infections and to identify the species of these dermatophytes at the Dermatological Clinic of the Hospital for Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok in a 1-year period...
May 17, 2017: New Microbiologica
https://www.readbyqxmd.com/read/28513811/an-initially-unidentified-case-of-urinary-tract-infection-due-to-aerococcus-urinae
#12
Georgios Meletis, Dimitrios Chatzidimitriou, Fani Tsingerlioti, Fani Chatzopoulou, Georgios Tzimagiorgis
Aerococcus urinae is a microorganism responsible for urinary tract and blood stream infections which are rarely reported in clinical practice. However, it has been proposed that the infrequency of such reports may be partially due to difficulties related to pathogen identification. We present here a case of an elderly male patient with urinary tract infection where A. urinae was initially not identified by a private microbiology laboratory. Our report highlights the need to consider A. urinae as a causative agent of urinary tract infections because if not identified and properly treated it may lead to endocarditis or septicemia...
May 17, 2017: New Microbiologica
https://www.readbyqxmd.com/read/28513810/epstein-barr-virus-encephalitis-in-solid-organ-transplantation
#13
Jillian S Y S Y, Zhi Mei Low, Iain Abbott, Lani Shochet, John Kanellis, Richard A Kitching, Tony M Korman
Epstein-Barr virus (EBV) is typically associated with post transplant lymphoproliferative disease (PTLD) after solid organ and stem cell transplantation. However, it is rarely associated with neurological complications. We report a case of severe encephalitis complicating primary EBV infection six months post renal transplantation, and review the literature on EBV encephalitis in solid organ transplantation in adults. A 55-year-old male presented 6 months post cadaveric renal transplant with headache, fever and confusion...
May 17, 2017: New Microbiologica
https://www.readbyqxmd.com/read/28513809/oral-gentamicin-therapy-for-carbapenem-resistant-klebsiella-pneumoniae-gut-colonization-in-hematologic-patients-a-single-center-experience
#14
Paola Lambelet, Carlo Tascini, Simona Fortunato, Alessandro Stefanelli, Federico Simonetti, Chiara Vettori, Alessandro Leonildi, Francesco Menichetti
The mortality for carbapenem-resistant Klebsiella pneumoniae (KPC-Kp) infection ranges from 18 to 48% depending on the type of therapy. Mortality rates in hematologic patients are even higher, up to 85%. Gut decontamination with oral gentamicin might be an option to avoid a subsequent KPC-Kp infection in colonized patients. We treated 14 hematologic patients with oral gentamicin, 80 mg four times daily, for 7 to 25 days in order to eradicate KPC-Kp from the gut, starting oral gentamicin therapy when possible after the discontinuation of systemic antibiotic therapy...
May 17, 2017: New Microbiologica
https://www.readbyqxmd.com/read/28513808/magnetic-nanoparticle-enhanced-pcr-for-the-detection-and-identification-of-staphylococcus-aureus-and-salmonella-enteritidis
#15
Dimitra Houhoula, Joseph Papaparaskevas, Katerina Zatsou, Nikolaos Nikolaras, Hanan I Malkawi, Marie-Paule Mingenot-Leclercq, Spyros Konteles, Stamatis Koussisis, Athanassios Tsakris, Ekatherina Charvalos
This paper evaluated magnetic nanoparticle-enhanced PCR for the detection and identification of Staphylococcus aureus and Salmonella enteritidis. Two different types of magnetic nanoparticles designated MPIO (iron concentration 2.5 mg/ml, size 1 μm) and NP (iron concentration 8.7 mg/ml, size 60 nm), both conjugated with S. aureus or S. enteritidis antibodies were evaluated as an enrichment procedure for PCR-detection of the pathogens in Trypticase Soy Broth, milk, blood and meat broth. Bacterial suspensions (1...
May 17, 2017: New Microbiologica
https://www.readbyqxmd.com/read/28513816/italian-guidelines-for-the-use-of-antiretroviral-agents-and-the-diagnostic-clinical-management-of-hiv-1-infected-persons-update-2016
#16
Andrea Antinori, Antonio Di Biagio, Simone Marcotullio, Massimo Andreoni, Antonio Chirianni, Antonella d'Arminio Monforte, Massimo Galli, Francesco Mazzotta, Cristina Mussini, Massimo Puoti, Adriano Lazzarin
The Italian Society of Infectious and Tropical Diseases (SIMIT) of the Technical Health Committee, Ministry of Health (Sections L and M) of Italy have supported recommendations for the Italian guidelines for the use of antiretroviral agents and the diagnostic-clinical management of HIV-1 infected persons. This publication summarizes the latest updates to the 2016 version of the Italian Guidelines for the management of HIV-1 infected patients and the use of antiretroviral drugs. In particular, new recommendations were released concerning the following topics: estimate of the HIV continuum of care in Italy, optimal timing and preferred drug combinations for starting antiretroviral therapy, treatment optimization, and pre-exposure prophilaxis (PrEP)...
April 2017: New Microbiologica
https://www.readbyqxmd.com/read/28368078/genotyping-of-toxoplasma-gondii-strain-directly-from-human-csf-samples-of-congenital-toxoplasmosis-clinical-case
#17
Chiara Pagliuca, Gabiria Pastore, Elena Scaglione, Annalisa Migliucci, Giuseppe Maria Maruotti, Annunziata Gaetana Cicatiello, Elena Salvatore, Marco Picardi, Josè Camilla Sammartino, Maria Consiglio Buonocore, Pasquale Martinelli, Emilia Iaccarino, Roberta Colicchio, Paola Salvatore
This report describes a case of congenital toxoplasmosis in a newborn in Southern Italy. A pregnant mother had been admitted at the 20th week of her pregnancy on account of pharyngodynia and laterocervical lymphadenopathy. Although serological testing of the mother's serum documented a seroconversion with positive IgG and IgM anti-Toxoplasma antibodies during II trimester, the woman refused to perform prenatal diagnosis for congenital toxoplasmosis. Fetal ultrasound scan already showed mild asymmetrical triventricular hydrocephaly and cerebral calcifications...
April 2017: New Microbiologica
https://www.readbyqxmd.com/read/28368077/cowpox-virus-infection-in-a-child-after-contact-with-a-domestic-cat-a-case-report
#18
Ryszard Żaba, Magdalena Jałowska, Michał J Kowalczyk, Monika Bowszyc-Dmochowska, Zygmunt Adamski, Andrzej Szkaradkiewicz
Human cowpox represents a seldom diagnosed zoonosis but this diagnosis should be considered more frequently as the number of cases has increased in recent years. We describe a case of cowpox in an 11-yearold boy following regular direct daily contact with a domestic cat. The 11-year-old patient, an otherwise healthy boy, demonstrated skin ulceration located at his chin, with enlargement of regional lymph nodes and fever reaching 39°C. The diagnosis of cowpox was made on the basis of PCR involving DNA isolated from a scab covering the skin lesion...
April 2017: New Microbiologica
https://www.readbyqxmd.com/read/28368076/antimicrobial-consumption-and-antimicrobial-resistance-a-snapshot-of-an-italian-neuromuscular-rehabilitation-center
#19
Giuseppina Barberi, Maria Cristina De Cola, De Cola Dell'Utri, Sara Melardi, Bernardo Alagna, Placido Bramanti, Antonio Cascio
The paper presents a snapshot of the incidence of antimicrobial-resistant microorganisms and antimicrobial consumption in an Italian rehabilitation center over a two-year period (2014-2015). Data on microorganism identification and antimicrobial susceptibility testing were obtained from the diagnostic laboratory of the hospital. A set of indicators was assessed, including the incidence density of resistant isolates per 1000 patient-days (IDRI). Data on antimicrobial consumption, semi-annually, obtained from the hospital pharmacy, were expressed as Defined Daily Dose (DDD) per 1000 patient-days...
April 2017: New Microbiologica
https://www.readbyqxmd.com/read/28368075/clinical-features-and-outcome-of-hospitalized-patients-with-hsv-1-dna-in-the-lower-respiratory-tract
#20
Francesca Pica, Marco Ciotti, Massimo Maurici, Cristina Buè, Paolo Nardi, Gabriella Lucà, Gabriella Moroni, Paola Rogliani, Carlo Federico Perno, Antonio Volpi
The aim of this study was to define the clinical impact of Herpes simplex virus-1 (HSV-1) DNA detection in the low respiratory tract of hospitalized patients. Forty-nine patients admitted to the University Hospital Tor Vergata, Rome, Italy, from May 2013 to June 2014, were analysed. Inclusion criteria were the presence or absence of HSV-1 DNA in clinical routine bronchoalveolar lavage (BAL) fluid specimens. Nineteen individuals were positive (cases) and 30 negative (controls) for the presence of HSV-1 DNA. The two groups were matched for age, gender and month of BAL collection...
April 2017: New Microbiologica
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