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Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology

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https://www.readbyqxmd.com/read/29775651/molecular-cloning-and-expression-analysis-of-interleukin-1%C3%AE-and-interleukin-1-receptor-type-i-genes-in-yellow-catfish-pelteobagrus-fulvidraco-responses-to-challenge-of-edwardsiella-ictaluri
#1
Chen-Lei Liao, Gui-Rong Zhang, Dong-Mei Zhu, Wei Ji, Ze-Chao Shi, Rui Jiang, Qi-Xue Fan, Kai-Jian Wei
Interleukin-1β (IL-1β) is one of the pivotal early pro-inflammatory cytokines, which play important roles in regulating immune response and inducing a series of inflammatory reactions to infections. Interleukin-1 type I receptor (IL-1RI) is a receptor of the IL-1β that can mediate IL-1-dependent activation. In this study, partial cDNA sequences of the Pf_IL-1β and Pf_IL-1RI genes were cloned from yellow catfish (Pelteobagrus fulvidraco). The open reading frames (ORF) of Pf_IL-1β and Pf_IL-1RI genes encode putative peptides of 280 and 543 amino acids, respectively...
May 15, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29698715/molecular-cloning-and-expression-profiles-of-igfbp-1a-in-common-carp-cyprinus-carpio-and-its-expression-regulation-by-growth-hormone-in-hepatocytes
#2
Wenbo Chen, Haoran Lin, Wensheng Li
In this study, we cloned and determined IGFBP-1a cDNA from common carp (Cyprinus carpio) liver. The 1655 bp full-length cDNA consisted of a 96 bp 5-untranslated region (UTR), a 789 bp open reading frame encoding 262 amino acid residues and a 770 bp 3-UTR containing seven mRNA instability motifs. Northern blot revealed a 1.8 kb IGFBP-1a transcript. IGFBP-1a mRNA was widely distributed in all tissues examined and predominantly expressed in the liver. During embryogenesis, IGFBP-1a mRNA was firstly observed in blastula stage, and significant increases were observed in body segment stage, lens formation stage and blood cycling stage...
April 23, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29694919/new-potential-biomarkers-of-oxidative-stress-in-mytilus-galloprovincialis-analytical-validation-and-overlap-performance
#3
Lorena Franco-Martínez, Diego Romero, Camila Peres Rubio, Fernando Tecles, Silvia Martínez-Subiela, Mariana Teles, Asta Tvarijonaviciute
The aim of the present report was to develop and validate new automated spectrophotometric assays for measurement of total antioxidant capacity (TAC), thiols, and advanced oxidation protein products (AOPP) in mussel gills, digestive gland and hemolymph samples, and to evaluate their possible utility in biomonitoring programs. Total antioxidant capacity (TAC) was measured by different methods: trolox equivalent antioxidant capacity (TEAC1 and TEAC2), cupric reducing antioxidant capacity (CUPRAC), and ferric reducing ability of plasma (FRAP)...
April 22, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29684538/molecular-cloning-characterization-and-expression-analysis-of-c-ebp-%C3%AE-%C3%AE-and-%C3%AE-in-adipose-related-tissues-and-adipocyte-of-duck-anas-platyrhynchos
#4
Jiamin Qiu, Wanxia Wang, Shenqiang Hu, Yushi Wang, Wenqiang Sun, Jiwei Hu, Jiwen Wang
CCAAT/enhancer binding protein α, β, δ (C/EBP α, β, δ) are essential transcriptional factors in regulating adipose development. However, information about their sequence characteristics and functions during adipocyte development still remains scarce in birds. In present study, we found that duck C/EBP α, β, δ differed in their phosphorylation sites and low complexity regions (LCRs) among their orthologs and paralogs. Phylogenetic analysis showed that C/EBP α, β, δ had different evolutionary patterns, and each of duck C/EBP α, β, δ was strikingly diverged from orthologs of other Aves...
April 20, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29655871/cloning-partial-sequencing-and-expression-analysis-of-the-neural-form-of-p450-aromatase-cyp19a1b-in-the-south-america-catfish-rhamdia-quelen
#5
Helena C Silva de Assis, Laia Navarro-Martín, Letícia S P Fernandes, Cibele C Cardoso, Daniela Parada Pavoni, Vance L Trudeau
Brain aromatase is a key enzyme exclusively expressed in fish radial glial cells that convert androgens into estrogens, thus controlling neuroendocrine functions and neurogenesis. As an important step in characterizing the neuroendocrine systems of Rhamdia quelen (jundiá), a partial cDNA sequence (1045 bp) of brain aromatase (cyp19a1b) was cloned and sequenced. At the nucleotide level the cDNA sequence was found to be 88% identical to cyp19a1b of two species of catfish, Ictalurus punctatus and Silurus meridionalis...
April 12, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29654886/role-of-abc-transporters-white-scarlet-and-brown-in-brown-planthopper-eye-pigmentation
#6
Yanyun Jiang, Xinda Lin
The brown planthopper ATP-binding cassette (ABC) proteins White (W), Scarlet (St) and Brown (Bw) belong to the ABC transporter superfamily and are responsible for the transportation of guanine and tryptophan precursors of eye pigments. In the present study, the brown planthopper White (NlW), S t(NlSt) and Bw (NlBw) genes were cloned, and subsequent phylogenetic analysis showed that these genes are clustered with their respective homologues, with a genetic relationship observed between NlW and its Bemisia tabaci homologue having the highest similarity...
April 11, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29649577/bioinformatics-characterization-of-a-cathepsin-b-transcript-from-the-giant-river-prawn-macrobrachium-rosenbergii-homology-modeling-and-expression-analysis-after-aeromonas-hydrophila-infection
#7
Saowalak Onming, Wilawan Thongda, Chao Li, Orathai Sawatdichaikul, Nichanun McMillan, Sirawut Klinbunga, Eric Peatman, Supawadee Poompuang
Cathepsin B is a lysosomal proteolytic enzyme that has been suggested to play a role in pathological processes of immune system. In this study, the full-length cDNA sequence of cathepsin B transcript in the giant river prawn Macrobrachium rosenbergii (MrCTSB) was obtained from 454 pyrosequencing of cDNAs from hepatopancreas and muscle. It was 1158 bp in length, containing an open reading frame (ORF) of 987 bp corresponding to 328 amino acids. The predicted molecular mass and pI of MrCTSB protein was 36...
April 9, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29580837/identification-and-characterization-of-two-distinct-sigma-class-glutathione-s-transferase-from-freshwater-bivalve-cristaria-plicata
#8
Zhenfang Li, Yuduan Cha, Baoqing Hu, Chungen Wen, Shaoqing Jian, Peipei Yi, Yang Gang
Glutathione-S-transferases (GSTs) are multifunctional phase II detoxification enzymes that catalyze the attachment of electrophilic substrates to glutathione, and play an important role in protecting organisms against the toxicity of reactive oxygen species. In this study, two distinct sigma-class GST (CpGSTσ1 and 2) cDNA sequences were cloned from freshwater bivalve Cristaria plicata. The full length cDNA of CpGSTσ1 and 2 was 826 bp and 1609 bp, which encoded 213 and 248 amino acid residues, respectively...
March 23, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29580836/differential-expression-of-two-piwil-orthologs-during-embryonic-and-gonadal-development-in-pufferfish-takifugu-fasciatus
#9
Xin Wen, Dan Wang, Xinru Li, Cheng Zhao, Tao Wang, Xiaoming Qian, Shaowu Yin
Piwil was an important regulator gene in germ cell division during gonadal development. Two Piwi-like genes, Piwil1 and Piwil2, were first cloned from T. fasciatus. The full-length cDNAs of Piwil1 and Piwil2 were of 2933 and 3394 bp, respectively. Piwil1 and Piwil2 possessed an open reading frame (ORF) of 2565 and 3138 bp, encoding 854 and 1045 amino acids, respectively. The tissue distribution analysis demonstrated that Piwil1 and Piwil2 were expressed at higher levels in gonad compared to other tissues (brain, liver, gill, etc...
March 23, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29567070/identification-of-duplicated-midkine-genes-and-their-functional-regulation-in-blunt-snout-bream-megalobrama-amblycephala
#10
Dan-Dan Guo, Guo-Dong Zheng, Shang-Ke Du, Bo Qin, Xia-Yun Jiang, Shu-Ming Zou
Midkine (Mdk) is a heparin-binding growth factor that is involved in regulating cell growth, differentiation and migration. Here, we report the isolation and characterization of duplicated mdk genes in blunt snout bream (Megalobrama amblycephala). The mdka and -b genes encode 146 aa and 147 aa peptides, respectively, sharing a sequence identity of 64%. During embryogenesis, mdka mRNA is detectable after 12 h post-fertilization (hpf) and mdkb mRNA can be detected after 8 hpf, about 4 h prior to mdka mRNA...
March 19, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29567069/the-short-term-presence-of-oleate-or-octanoate-alters-the-phosphorylation-status-of-akt-ampk-mtor-creb-and-foxo1-in-liver-of-rainbow-trout-oncorhynchus-mykiss
#11
Cristina Velasco, Sara Comesaña, Marta Conde-Sieira, Jesús M Míguez, José L Soengas
We hypothesized that the presence of specific fatty acids modulate lipid metabolism in fish liver through changes in the phosphorylation status of proteins involved in cell signaling (Akt and mTOR) and cellular energy sensing (AMPK), and transcription factors (CREB and FoxO1). Therefore, we evaluated in pools of rainbow trout livers incubated for 3 h and 6 h at 15 °C in modified Hanks' medium containing 100 or 500 μM oleate or octanoate, the response of these proteins. Our data confirm, for the first time in fish, that treatment of rainbow trout liver with oleate or octanoate resulted in the modulation of phosphorylation status of proteins involved in cell signaling (increased Akt and mTOR) and energy sensing (decreased AMPK), and in transcription factors (decreased CREB and increased FoxO1)...
March 19, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29555304/functional-characterization-of-chitin-deacetylase-1-gene-disrupting-larval-pupal-transition-in-the-drugstore-beetle-using-rna-interference
#12
Wen-Jia Yang, Kang-Kang Xu, Xin Yan, Chun-Xu Chen, Yu Cao, Yong-Lu Meng, Can Li
Chitin deacetylases (CDAs) are chitin degradation enzymes that strictly regulate growth and development in insects. In this study, we identified and characterized a full-length cDNA of the CDA gene (SpCDA1) in the drugstore beetle, Stegobium paniceum. The open reading frame of SpCDA1 (1614 bp) encoded a 537 amino acid protein, which possessed typical domain structures of CDAs. Phylogenetic comparison to other insect CDAs revealed that SpCDA1 belongs to Group Ib CDAs. Quantitative real-time PCR analyses showed that SpCDA1 was highly expressed in late larval stages...
March 16, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29501788/50-years-of-comparative-biochemistry-the-legacy-of-peter-hochachka
#13
L T Buck, G Burness, K L Campbell, C-A Darveau, W Driedzic, H Guderley, G B McClelland, T W Moon, C D Moyes, P M Schulte
Peter Hochachka was an early pioneer in the field of comparative biochemistry. He passed away in 2002 after 4 decades of research in the discipline. To celebrate his contributions and to coincide with what would have been his 80th birthday, a group of his former students organized a symposium that ran as a satellite to the 2017 Canadian Society of Zoologists annual meeting in Winnipeg, Manitoba (Canada). This Special Issue of CBP brings together manuscripts from symposium attendees and other authors who recognize the role Peter played in the evolution of the discipline...
February 28, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29501789/a-lesson-from-the-oxidative-metabolism-of-hibernator-heart-possible-strategy-for-cardioprotection
#14
Ana Stancic, Aleksandra Jankovic, Aleksandra Korac, Dusko Cirovic, Vesna Otasevic, Kenneth B Storey, Bato Korac
In the present study we hypothesized that myocardial adaptive phenotype in mammalian hibernation involves rearrangement of mitochondria bioenergetic pathways providing protective pattern in states of reduced metabolism and low temperature. European ground squirrels (Spermophilus citellus) were exposed to low temperature (4 ± 1 °C) and then divided into two groups: (1) animals that fell into torpor (hibernating group) and (2) animals that stayed active and euthermic for 1, 3, 7, 12, or 21 days (cold-exposed group)...
May 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29486246/the-dna-methylation-status-of-myod-and-igf-i-genes-are-correlated-with-muscle-growth-during-different-developmental-stages-of-japanese-flounder-paralichthys-olivaceus
#15
Yajuan Huang, Haishen Wen, Meizhao Zhang, Nan Hu, Yufeng Si, Siping Li, Feng He
Many genes related to muscle growth modulate myoblast proliferation and differentiation and promote muscle hypertrophy. MyoD is a myogenic determinant that contributes to myoblast determination, and insulin-like growth factor 1 (IGF-I) interacts with MyoD to regulate muscle hypertrophy and muscle mass. In this study, we aimed to assess DNA methylation and mRNA expression patterns of MyoD and IGF-I during different developmental stages of Japanese flounder, and to examine the relationship between MyoD and IGF-I gene...
May 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29496579/visual-pigment-genes-and-absorbance-spectra-in-the-japanese-sardine-sardinops-melanostictus-teleostei-clupeiformes
#16
Taeko Miyazaki, Sergei L Kondrashev, Tohru Tsuchiya
The spectral absorbance of photoreceptor visual pigments and the opsin gene class of the visual pigments was investigated in Sardinops melanostictus. Microspectrophotometric (MSP) measurements showed that the rod photoreceptors had peak absorbance spectra (λmax ) at 502 nm. The spectral sensitivity of single cones was centered at 393 nm. Double cones had a λmax of 493/522 nm, but a few displayed a red-shifted absorbance of the long-wave member at 542 nm. The mRNAs of six different opsins were isolated from the retina, retrotranscribed, cloned, and sequenced...
April 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29496578/molecular-cloning-expression-pattern-of-follistatin-gene-and-association-analysis-with-growth-traits-in-bighead-carp-hypophthalmichthys-nobilis
#17
Meixia Pang, Jingou Tong, Xiaomu Yu, Beide Fu, Ying Zhou
Follistatin (FST) is a single-chain gonadal protein involving in various biological effects. FST plays important roles in not only ovary development but also body growth, whereas myostatin (MSTN) negatively regulates muscle growth. In this study, FST gene in bighead carp (HynFST) was cloned and characterized. A 5797 bp genomic sequence of HynFST, consisting six exons and five introns were cloned. The full-length cDNA of HynFST (2134 bp) has an open reading fragment encoding a polypeptide of 349 amino acids...
April 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29452261/maternal-betaine-administration-modulates-hepatic-type-1-iodothyronine-deiodinase-dio1-expression-in-chicken-offspring-through-epigenetic-modifications
#18
Zhen Hou, Qinwei Sun, Yun Hu, Shu Yang, Yibo Zong, Ruqian Zhao
As a feed additive, betaine is widely used in livestock production for its ability to promote growth. Our previous studies had reported that maternal betaine supplementation altered hepatic metabolism in offspring. But it remains unknown whether and how maternal betaine modulates metabolism of thyroid hormones in the offspring chickens by epigenetic modification. In this study, one hundred and twenty Rugao yellow-feathered laying hens were randomly divided into two groups, and were fed basal diet with or without 0...
April 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29448011/oceanographic-upwelling-conditions-influence-signaling-pathways-involved-in-muscle-growth-of-intertidal-fish
#19
Rodrigo Zuloaga, Oscar Almarza, Juan A Valdés, Alfredo Molina, José Pulgar
Few studies have addressed the impact of the upwelling oceanographical conditions on biological processes, such as growth, using a molecular and physiological approach. Upwelling conditions are characterized by low temperature seawater and high nutrient availability, which represents an ideal opportunity to understand how habitat modulates animal performance at different levels of biological complexity. We aimed to assess intraspecific variations in weight, oxygen consumption, protein content, and key signaling pathways involved in muscle-growth (protein kinase B (AKT) and extracellular signal-regulated kinase (ERK)) under experimental trials considering high/low seawater temperatures with full/restricted food rations...
April 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29427614/gene-expression-and-activity-of-digestive-enzymes-of-daphnia-pulex-in-response-to-food-quality-differences
#20
Anke Schwarzenberger, Patrick Fink
Food quality is an important factor influencing organisms' well-being. In freshwater ecosystems, food quality has been studied extensively for the keystone herbivore genus Daphnia, as they form the critical trophic link between primary producers and higher order consumers such as fish. For Daphnia, the edible fraction of phytoplankton in lakes (consisting mostly of unicellular algae and cyanobacteria) is extraordinarily diverse. To be able to digest different food particles, Daphnia possess a set of digestive enzymes that metabolize carbohydrates, lipids and proteins...
April 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
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