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Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology

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https://www.readbyqxmd.com/read/28636949/cloning-expression-and-nutritional-regulation-of-the-glutamine-synthetase-gene-in-ctenopharyngodon-idellus
#1
Rong Hu, Fufa Qu, Jianzhou Tang, Qiong Zhao, Jinpeng Yan, Zhigang Zhou, Yi Zhou, Zhen Liu
Glutamine synthetase (GS) is considered a master enzyme that catalyses ATP-dependent biosynthesis of glutamine from glutamate. In the present study, the GS gene was cloned from the intestine of grass carp, (Ctenopharyngodon idellus). The full-length cDNA sequence of GS encodes a 371-amino-acid polypetide. Phylogenetic analysis of the C. idellus GS sequence reveals common carp (Cyprinus carpio) as its closest neighbor. GS mRNA was differentially expressed in different tissues, with high to low gradient expression the intestine, brain, muscle, heart, gill, liver, pituitary gland, and spleen...
June 18, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28630013/receptor-mediated-endocytosis-of-vicilin-in-callosobruchus-maculatus-coleoptera-chrysomelidae-larval-midgut-epithelial-cells
#2
Daniele Kunz, Gabriel B Oliveira, Adriana F Uchôa, Richard I Samuels, Maria Lígia R Macedo, Carlos P Silva
The transport of proteins across the intestinal epithelium of insects is still not well understood. There is evidence that vicilin, a major storage protein of cowpea seeds (Vigna unguiculata), is internalized in larvae of the seed-beetle Callosobruchus maculatus. It has been reported that this vicilin interacts with proteins present in the microvillar membranes of columnar cells along the digestive tract of the larvae. In the present work, we studied the cellular pathway involved in endocytosis of vicilin in larval C...
June 16, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28630012/assessing-passeriforme-health-in-south-texas-via-select-venous-analytes
#3
J Pistone, J J Heatley, T A Campbell, G Voelker
The handheld point of care analyzer is a quick and feasible option to obtain hematology data from individuals. The iSTAT-1® was used to evaluate select venous blood analytes obtained via jugular venipuncture from 238 passerine birds from South Texas. These data were used to assess the health of birds in the area while taking into consideration life history (migratory or sedentary), locale, seasonality, sex, and age. We attributed increased values of pO2 and hematocrit, in addition to hemoglobin and glucose concentrations of migratory birds compared to sedentary birds as the increased need of oxygen carrying capacity and energy for long duration flights...
June 16, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28625796/the-characterization-of-rheb-gene-and-its-responses-to-hypoxia-and-thermal-stresses-in-the-small-abalone-haliotis-diversicolor
#4
Lianghua He, Xin Zhang, Ying Huang, Huiping Yang, Yilei Wang, Ziping Zhang
RHEB (Ras Homolog Enriched in Brain) is a GTP-binding protein that is ubiquitously expressed in humans and other mammals. The protein is largely involved in the mechanistic target of rapamycin (mTOR) pathway, and regulates the cell cycle progression and growth. The goal of this study was to characterize the RHEB gene in the small abalone Haliotis diversicolor, and identify the responses of RHEB gene to stresses of hypoxia or/and thermal. The objectives were to: 1) clone the full-length cDNA RHEB gene in the H...
June 15, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28602421/endocrine-disruption-effects-in-male-and-intersex-roach-rutilus-rutilus-l-from-french-rivers-an-integrative-approach-based-on-subcellular-to-individual-responses
#5
Perrine Geraudie, Marie Gerbron, Christophe Minier
Among the chemicals found in the aquatic environment, some have been reported to interact with the endocrine system of organisms and have been identified as endocrine disrupting compounds. In this study, we have assessed the biological effects due to environmental estrogenic compounds in adult male roach (Rutilus rutilus). Wild fish were collected in the Seine river, and one of its affluent, the Epte river in Normandy, France and impacts on endocrine and reproductive functions have been assessed. This study provides innovative data on the intersex condition in wild fish that are crucial to understand estrogenic contamination impacts and maintenance of sustainable fish population...
June 5, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28552377/influence-of-water-salinity-on-genes-implicated-in-somatic-growth-lipid-metabolism-and-food-intake-in-pejerrey-odontesthes-bonariensis
#6
Juan Ignacio Bertucci, Mario Oswaldo Tovar, Ayelén Melisa Blanco, Pedro Gómez-Requeni, Suraj Unniappan, Luis Fabián Canosa
Pejerrey, Odontesthes bonariensis, is an euryhaline fish of commercial importance in Argentina. This work aimed to determine if water salinity affects the expression of genes involved in somatic growth (gh; ghr-I; ghr-II; igf-I), lipid metabolism (Δ6-desaturase) and food intake (nucb2/nesfatin-1). First, we identified the full-length cDNA sequences of Δ6-desaturase (involved in lipid metabolism) and nesfatin-1 (an anorexigen). Then, pejerrey juveniles were reared during 8weeks in three different water salinity conditions: 2...
May 26, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28529085/variations-in-native-protein-glycation-and-plasma-antioxidants-in-several-birds-of-prey
#7
Tana Ingram, Jessica Zuck, Chad R Borges, Patrick Redig, Karen L Sweazea
Birds are an anomaly among vertebrates as they are remarkably long-lived despite having naturally high blood glucose and metabolic rates. For mammals, hyperglycemia leads to oxidative stress and protein glycation. In contrast, many studies have shown that domestic and wild birds are relatively resistant to these glucose-mediated pathologies. Surprisingly very little research has examined protein glycation in birds of prey, which by nature consume a diet high in protein and fat that promotes gluconeogenesis...
May 19, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28502832/sexually-dimorphic-expression-in-developing-and-adult-gonads-shows-an-important-role-of-gonadal-soma-derived-factor-during-sex-differentiation-in-olive-flounder-paralichthys-olivaceus
#8
Yuezhong Liu, Wei Zhang, Xinxin Du, Jun Zhao, Xiaobing Liu, Xiaojing Li, Quanqi Zhang, Xubo Wang
Gonadal soma-derived factor (gsdf) is a new member of transforming growth factor beta (TGF-β) superfamily. As a teleost- and gonad-specific growth factor, gsdf has been indicated to play an important role in early germ cell development. However, little is known about its significance in germ cell development of olive flounder (Paralichthys olivaceus). In the present study, a 1338 bp gsdf gene was isolated from P. olivaceus for the first time. Bioinformatic analysis revealed that the genomic structure and synteny relationship of gsdf in teleosts were conserved...
May 11, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28499851/effects-of-nutritional-history-on-stress-response-in-gibel-carp-carassius-auratus-gibelio-and-largemouth-bass-micropterus-salmoides
#9
Danli Jiang, Yubo Wu, Di Huang, Xing Ren, Yan Wang
The stress response of omnivorous gibel carp (Carassius auratus gibelio) and carnivorous largemouth bass (Micropterus salmoides) with different nutritional history were evaluated. A 2×2 layout, including two fish species (gibel carp or largemouth bass) and two nutritional history (fasted or fed to satiation for four weeks), was used. After feeding or fasting, the fishes were subjected to an acute handling. Fasting resulted in decrease of plasma glucose level and liver glycogen content of gibel carp and largemouth bass...
May 10, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28499850/skeletal-muscle-protease-activities-in-the-early-growth-and-development-of-wild-atlantic-salmon-salmo-salar-l
#10
Liudmila A Lysenko, Nadezda P Kantserova, Elena I Kaivarainen, Marina Yu Krupnova, Nina N Nemova
Growth-related dynamics of intracellular protease activities in four year classes of the Atlantic salmon (Salmo salar L. 1758) parr and smolts inhabiting salmon rivers of northwestern Russia (the White Sea basin) were studied. Cathepsin B, cathepsin D, proteasome, and calpain activities in the skeletal muscles of salmon were assessed to investigate their relative contribution to the total protein degradation as well as to young fish growth process. It was confirmed that calpain activity dominate in salmon muscles while proteasome plays a minor role, in contrast to terrestrial vertebrates...
May 9, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28408166/age-and-stage-dependent-variations-of-muscle-specific-gene-expression-in-brown-trout-salmo-trutta-l
#11
Maria V Churova, Olga V Meshcheryakova, Michail Ruchev, Nina N Nemova
This study was conducted to characterize the features of muscle-specific genes expression during development of brown trout Salmo trutta inhabiting the river Krivoy ruchey (Kola Peninsula, Russia). Gene expression levels of myogenic regulatory factors (MRFs - MyoD1 paralogs (MyoD1a, MyoD1b, MyoD1c), Myf5, myogenin), myostatin paralogs (MSTN-1a, MSTN-1b, MSTN-2a), fast sceletal myosin heavy chain (MyHC) were measured in the white muscles of brown trout parr of ages 0+ (under-yearling), 1+ (yearling) and 2+ (two year old) and smolts of age 2+...
April 10, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28438684/two-spliced-isoforms-of-the-sex-determination-gene-fruitless-in-the-chinese-mitten-crab-eriocheir-sinensis
#12
Peiyao Li, Yuan Liu, Danli Luo, Chengwen Song, Zhaoxia Cui
The fruitless (fru) gene plays an important role in the sex determination pathway and courtship behavior of Drosophila melanogaster. In the present study, two fru isoforms (Esfru1 and Esfru2) were identified from the Chinese mitten crab Eriocheir sinensis. Sequence analysis showed that Esfru1 and Esfru2 were encoded by the same genomic locus and generated by alternative splicing of pre-mRNA. Esfru1 had all introns completely spliced out, while Esfru2 had a longer exon1 with an additional 78bp sequence. They both contained a conserved BTB domain which was also found in D...
June 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28438683/identification-and-expression-of-piwil2-in-turbot-scophthalmus-maximus-with-implications-of-the-involvement-in-embryonic-and-gonadal-development
#13
Huizhen Wang, Bo Wang, Xiaobing Liu, Yuezhong Liu, Xinxin Du, Quanqi Zhang, XuBo Wang
Piwil2, a member of the Argonaute family, is involved in the biogenesis of PIWI-interacting RNAs (piRNAs) and plays an important role in regulating gametogenesis. In the present study, we identified turbot Scophthalmus maximus piwil2 gene, named Smpiwil2, which contained a PAZ domain and a PIWI domain. Sequence comparison, genomic structure and phylogenetic analyses showed that Smpiwil2 is homologous to that of teleosts and tetrapods. The Smpiwil2 transcript showed higher expression in the ovary than in the testis, demonstrating a sexually dimorphic gene expression pattern...
June 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28427900/molecular-cloning-and-characterization-of-kiss1-in-brandt-s-voles-lasiopodomys-brandtii
#14
Yan Chen, Lan Liu, Zhengguang Li, Dawei Wang, Ning Li, Ying Song, Cong Guo, Xiaohui Liu
Kisspeptin, encoded by kiss1, has been regarded as a major modulator of mammalian puberty and fertility due to its stimulation on GnRH. Brandt's vole is one of the main pest species on the Inner Mongolian steppes for its striking reproductive capacity and kiss1 is a key candidate gene related to reproductive regulatory cascades. In this study, kiss1 cDNA was cloned from the hypothalamus of Brandt's voles and kiss1 mRNA levels were investigated in different tissues, and at different developmental stages, using high-throughput real-time PCR...
June 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28414176/genome-specific-ppar%C3%AE-b-duplicates-in-salmonids-and-insights-into-estrogenic-regulation-in-brown-trout
#15
Tânia Vieira Madureira, Ivone Pinheiro, Rafaelle de Paula Freire, Eduardo Rocha, Luis Filipe Castro, Ralph Urbatzka
Peroxisome proliferator-activated receptors (PPARs) are key regulators of many processes in vertebrates, such as carbohydrate and lipid metabolism. PPARα, a member of the PPAR nuclear receptor gene subfamily (NR1C1), is involved in fatty acid metabolism, namely in peroxisomal β-oxidation. Two gene paralogues, pparαA and pparαB, were described in several teleost species with their origin dating back to the teleost-specific genome duplication (3R). Given the additional salmonid-specific genome duplication (4R), four genes could be theoretically anticipated for this gene subfamily...
June 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28408167/purification-characterization-and-expression-of-transferrin-from-rainbow-trout-seminal-plasma
#16
Joanna Nynca, Mariola A Dietrich, Mikołaj Adamek, Dieter Steinhagen, Barbara Bilińska, Anna Hejmej, Andrzej Ciereszko
Transferrin (TF) is recognized as a multifunctional protein and has been implicated in antioxidative, antimicrobial protection, growth, differentiation and cytoprotection effects. An efficient, original three-step isolation procedure for TF consisting in hydrophobic interaction chromatography, gel filtration and preparative electrophoresis was developed. Rainbow trout TF was found to be N-glycosylated (not O-glycosylated) and phosphorylated at all serine, threonine, and tyrosine residues. The protein consists of several proteoforms with an average molecular weight of 76...
June 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28400332/sexual-dimorphic-expression-of-dnd-in-germ-cells-during-sex-reversal-and-its-requirement-for-primordial-germ-cell-survival-in-protogynous-hermaphroditic-grouper
#17
Zhi-Hui Sun, Li Zhou, Zhi Li, Xiao-Chun Liu, Shui-Sheng Li, Yang Wang, Jian-Fang Gui
Dead end (dnd), vertebrate-specific germ cell marker, had been demonstrated to be essential for primordial germ cell (PGC) migration and survival, and the link between PGC number and sex change had been revealed in some teleost species, but little is known about dnd in hermaphroditic vertebrates. In the present study, a protogynous hermaphroditic orange-spotted grouper (Epinephelus coioides) dnd homologue (Ecdnd) was identified and characterized. Quantitative real-time PCR and in situ hybridization analysis revealed a dynamic and sexually dimorphic expression pattern in PGCs and germ cells of gonads...
June 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28400331/four-paralog-gelsolin-genes-are-differentially-expressed-in-the-earthworm-lumbricus-terrestris
#18
COMPARATIVE STUDY
Prasath Thiruketheeswaran, Paul Thomalla, Evelyn Krüger, Horst Hinssen, Jochen D'Haese
We have identified and characterized four distinct variants of the gelsolin-related protein (EWAM P1-P4) in the earthworm L. terrestris. All of these proteins biochemically qualify as gelsolins since they sever actin filaments in a calcium dependent manner. P1, P2 and P3 are present in the Lumbricus body wall muscle whereas in the gizzard muscle P3 and P4 were found. P1-P4 are encoded by four paralog genes and are differentially expressed in various muscle cell tissues. While the genes for P1 and P2 contain one intron, there was no intron in both P3 and P4 genes...
June 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28377114/uptake-and-selective-partitioning-of-dietary-lipids-to-ovarian-and-muscle-tissue-of-maturing-female-coho-salmon-oncorhynchus-kisutch-during-secondary-oocyte-growth
#19
Ronald B Johnson, Eric L Kroeger, William L Reichert, Cameron S Carter, Michael B Rust
Female coho salmon, Oncorhynchus kisutch, were fed one of two experimental feeds containing lipids with markedly different stable (13)C isotope signatures during the late cortical alveolus, lipid droplet, and vitellogenesis stages of secondary oocyte growth. Ovarian and muscle lipids fatty acid concentrations were significantly affected by treatment during all three stages of development. Stable (13)C isotope analyses confirmed that dietary lipids were incorporated into both ovarian and muscle lipids during all three stages and revealed that ovarian lipids were more affected than muscle lipids during vitellogenesis...
June 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28373120/molecular-cloning-mrna-expression-and-nutritional-regulation-of-a-%C3%AE-6-fatty-acyl-desaturase-like-gene-of-mud-crab-scylla-paramamosain
#20
COMPARATIVE STUDY
Zhideng Lin, Meilin Hao, Dashi Zhu, Shengkang Li, Xiaobo Wen
Fatty acyl desaturases (Fads) are critical enzymes in the pathways for the biosynthesis of the highly unsaturated fatty acids (HUFA). Here we report on the molecular cloning, tissue expression and nutritional regulation of a Δ6 fatty acyl desaturase-like (Δ6 Fad-like) gene from mud crab, Scylla paramamosain. The full-length cDNA was 1973bp, with a 201bp of 5'-UTR, a 443bp of 3'-UTR, and an ORF of 1329bp that encoded a protein of 442 amino acids. Bioinformatics analysis showed that the deduced peptide sequence possessed the typical features of the microsomal Fads, including N-terminal cytochrome b5 domain containing the heme-binding motif (H-P-G-G), three histidine-rich boxes and three membrane-spanning regions...
June 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
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