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Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology

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https://www.readbyqxmd.com/read/30003958/characterization-and-localization-of-primordial-germ-cells-in-totoaba-macdonaldi
#1
Raquel Escuredo-Vielba, Miguel A Del Río-Portilla, Jose Antonio Mata-Sotres, Benjamín Barón-Sevilla, Yanet Guerrero-Rentería, Carmen G Paniagua-Chávez
The totoaba (Totoaba macdonaldi), is an endemic and endangered fish of the Gulf of California with high economic and ecological potential. Therefore, our purpose was to characterize the Primordial Germ Cells (PGCs) of this Sciaenid with two objectives: (1) to provide the basis for PGCs cryopreservation to preserve the genetic resources and (2) to take the first step to know the gonadal genesis and sex differentiation of totoaba. Immunofluorescence analysis performed from 2-cell stage to 8-day after hatch (DAH) shows that Vasa protein is specific for PGCs...
July 9, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29981453/identification-and-expression-of-cathepsin-b-from-the-freshwater-mussel-cristaria-plicata
#2
P Yi, X Hu, B Hu, C Wen, Z Li
Cathepsin B plays crucial roles in host immune defense against pathogen infection. In present study, a cathepsin B gene from the freshwater mussel, Cristaria plicata (CpCathB) was cloned and characterized. The full-length cDNA of CpCathB was 1825 bp, and contained a 5' untranslated region (UTR) of 36 nucleotides, an open reading frame (ORF) of 1044 bp and a 3' UTR of 745 bp with a poly (A) tail. The deduced CpCathB protein was encoded as a preproenzyme with 347 amino acid residues and predicted molecular weight of 38...
July 4, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29981452/molecular-and-functional-analysis-of-pmchst1b-in-nacre-formation-of-pinctada-fucata-martensii
#3
Ruijuan Hao, Zhe Zheng, Qingheng Wang, Xiaodong Du, Yuewen Deng, Ronglian Huang
Keratan sulfate possesses considerable amounts of negatively charged sulfonic acid groups and participates in biomineralization. In the present study, we investigated characteristics and functions of a CHST1 gene identified from the pearl oyster Pinctada fucata martensii (PmCHST1b) which participated in the synthesis of keratan sulfate. PmCHST1b amino acid sequence carried a typical sulfotransferase-3 domain (sulfotransfer-3 domain) and belonged to membrane-associated sulfotransferases. Homologous analysis of CHST1 from different species showed the conserved motif (5' PSB motif and 3' PB motif) which interacted with 3'-phosphoadenosine-5'-phosphosulfate (PAPS)...
July 4, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29981451/characterization-of-transforming-growth-factor-beta-regulator-1-like-and-association-between-its-expression-levels-and-growth-of-the-giant-tiger-shrimp-penaeus-monodon
#4
Sirawut Klinbunga, Sirithorn Janpoom, Puttawan Rongmung, Sirikan Prasertlux, Vipavadee Srisuvan, Piamsak Menasveta, Bavornlak Khamnamtong
Molecular markers that allow selection of juveniles and broodstock with improved growth performances are useful for the shrimp industry. Here, the full-length cDNA of transforming growth factor beta regulator 1-like (PmTbrg1-l) in the giant tiger shrimp Penaeus monodon was determined. It was 1184 bp in length and contained an open reading frame (ORF) of 975 bp corresponding to a deduced polypeptide of 324 amino acids. Successful RNA interference (RNAi) carried out using juveniles injected with PmTbrg1-l dsRNA revealed reduced levels of PmTbrg1-l and myostatin (PmMstm) in hemocytes when compared to shrimp injected with saline solution and GFP dsRNA (P < ...
July 4, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29966774/dietary-restriction-alters-the-fatbody-transcriptome-during-immune-responses-in-bombyx-mori
#5
Ye Pan, Peng Lü, Feifei Zhu, Chenjun Li, Yuanqing He, Keping Chen
Dietary restriction (DR) leads to extended lifespan in many species ranging from yeasts to mammal, and it can also affect the immune system to some extent. Herein, we investigated whether DR can enhance the immunity of Bombyx mori suffering from acute pathogenic microorganism infection. The results showed that DR could accelerate the melanisation reaction, delay the early death in silkworms, meanwhile Staphylococcus aureus (SA) load was lower in the early stage of infection. Moreover, more immune-related genes were identified to be down-regulated in the DR group infected with SA compared with the ad libitum - fed (AL) group infected with SA through mRNA deep sequencing (RNAseq) and quantitation PCR...
June 29, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29966773/host-plant-derived-allelochemicals-and-metal-components-are-associated-with-oxidative-predominance-and-antioxidant-plasticity-in-the-larval-tissues-of-silkworm-antheraea-mylitta-further-evidence-of-joint-effects-hypothesis
#6
Smaranika Sahu, Abinash Dutta, Dinesh Kumar Ray, Jyotsnarani Pradhan, Jagneshwar Dandapat
Tasar silkworm, Antheraea mylitta is a polyphagous insect that primarily feeds on Terminalia arjuna, Terminalia tomentosa and Shorea robusta. However, larval rearing on S. robusta results in poor performance for the reasons unexplored. Oxidative burden imposed by host plants is presumed to be a determining factor for larval fitness. With this hypothesis we have analyzed the foliar constituents of the respective host plants, the levels of oxidative stress and antioxidant protection in the larval tissues in response to their altered feeding on different host plants for different durations (2 and 10 days)...
June 29, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29960082/monitoring-changing-cellular-characteristics-during-the-development-of-a-fin-cell-line-from-cyprinus-carpio
#7
Sebastian Rakers, Anne-Kathrin Ondrusch, Martina Gruening, Mikolaj Adamek, Benita Moeckel, Marina Gebert
The establishment and in-depth characterization of a novel continuous cell line derived from fin tissue of common carp (Cyprinus carpio), CCApin, is reported. The cells of the cell line could be propagated in Leibovitz's L-15 medium containing 15% foetal calf serum and 0.5% carp serum for >150 passages during the last 24 months, with a stable fast growth. Furthermore, antibody staining indicated that cell types obtained in primary cultures, containing the epithelial stem-cell marker tumorprotein 63, were different from cells in long-term cell cultures, containing tight junction protein zona occludens 1 and cytokeratin 7...
June 27, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29894736/torpor-arousal-cycles-in-syrian-hamster-heart-are-associated-with-transient-activation-of-the-protein-quality-control-system
#8
Marit Wiersma, Thais M A Beuren, Edwin L de Vrij, Vera A Reitsema, Jantje J Bruintjes, Hjalmar R Bouma, Bianca J J M Brundel, Robert H Henning
Hibernation consists of torpor, with marked suppression of metabolism and physiological functions, alternated with arousal periods featuring their full restoration. The heart is particularly challenged, exemplified by its rate reduction from 400 to 5-10 beats per minute during torpor in Syrian hamsters. In addition, during arousals, the heart needs to accommodate the very rapid return to normal function, which lead to our hypothesis that cardiac function during hibernation is supported by maintenance of protein homeostasis through adaptations in the protein quality control (PQC) system...
June 9, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29883689/molecular-characterization-of-na-k-2cl-cotransporter-1-alpha-from-trachinotus-ovatus-linnaeus-1758-and-its-expression-responses-to-acute-salinity-stress
#9
Chao-Ping Zhao, Hua-Yang Guo, Ke-Cheng Zhu, Liang Guo, Nan Zhang, Bao-Suo Liu, Jing-Wen Yang, Bo Liu, Shi-Gui Jiang, Dian-Chang Zhang
Trachinotus ovatus is a stenohaline fish and widely cultured in the ponds and gulf on the southeast coast of China. The dramatic salinity decrease caused by heavy rainfall could cause mass mortality of T. ovatus in aquaculture. It is very important to understand the osmoregulatory mechanism of T. ovatus. Na+ /K+ /2Cl- cotransporter 1a (NKCC1a) is involved in the osmoregulation of fish and plays a crucial role in cell volume homeostasis and maintenance of the electrolyte content. In this study, we characterized nkcc1a (designed as Tonkcc1a) from T...
June 5, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29864501/dna-methylation-levels-and-expression-patterns-of-smyd1a-and-smyd1b-genes-during-metamorphosis-of-the-japanese-flounder-paralichthys-olivaceus
#10
Shuxian Wu, Yajuan Huang, Siping Li, Haishen Wen, Meizhao Zhang, Jifang Li, Yun Li, Changwei Shao, Feng He
Japanese flounder (Paralichthys olivaceus) undergoes metamorphosis by changing its body from the larval to the juvenile form, and this process involves muscle development. Smyd1, a histone methyltransferase, plays a role in the skeletal muscle. In the present study, the Smyd1a and Smyd1b expression patterns and their 5' UTR and exon 1 DNA methylation levels were analyzed during metamorphosis of the Japanese flounder. Sample were analyzed 21 days post-hatching (dph) (with no migration of right eye; M1stage), 28 dph (during migration of right eye; M2 stage), and 35 dph (after migration of right eye; M3 stage)...
June 1, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29775651/molecular-cloning-and-expression-analysis-of-interleukin-1%C3%AE-and-interleukin-1-receptor-type-i-genes-in-yellow-catfish-pelteobagrus-fulvidraco-responses-to-challenge-of-edwardsiella-ictaluri
#11
Chen-Lei Liao, Gui-Rong Zhang, Dong-Mei Zhu, Wei Ji, Ze-Chao Shi, Rui Jiang, Qi-Xue Fan, Kai-Jian Wei
Interleukin-1β (IL-1β) is one of the pivotal early pro-inflammatory cytokines, which play important roles in regulating immune response and inducing a series of inflammatory reactions to infections. Interleukin-1 type I receptor (IL-1RI) is a receptor of the IL-1β that can mediate IL-1-dependent activation. In this study, partial cDNA sequences of the Pf_IL-1β and Pf_IL-1RI genes were cloned from yellow catfish (Pelteobagrus fulvidraco). The open reading frames (ORF) of Pf_IL-1β and Pf_IL-1RI genes encode putative peptides of 280 and 543 amino acids, respectively...
May 22, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29698715/molecular-cloning-and-expression-profiles-of-igfbp-1a-in-common-carp-cyprinus-carpio-and-its-expression-regulation-by-growth-hormone-in-hepatocytes
#12
Wenbo Chen, Haoran Lin, Wensheng Li
In this study, we cloned and determined IGFBP-1a cDNA from common carp (Cyprinus carpio) liver. The 1655 bp full-length cDNA consisted of a 96 bp 5-untranslated region (UTR), a 789 bp open reading frame encoding 262 amino acid residues and a 770 bp 3-UTR containing seven mRNA instability motifs. Northern blot revealed a 1.8 kb IGFBP-1a transcript. IGFBP-1a mRNA was widely distributed in all tissues examined and predominantly expressed in the liver. During embryogenesis, IGFBP-1a mRNA was firstly observed in blastula stage, and significant increases were observed in body segment stage, lens formation stage and blood cycling stage...
July 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29694919/new-potential-biomarkers-of-oxidative-stress-in-mytilus-galloprovincialis-analytical-validation-and-overlap-performance
#13
Lorena Franco-Martínez, Diego Romero, Camila Peres Rubio, Fernando Tecles, Silvia Martínez-Subiela, Mariana Teles, Asta Tvarijonaviciute
The aim of the present report was to develop and validate new automated spectrophotometric assays for measurement of total antioxidant capacity (TAC), thiols, and advanced oxidation protein products (AOPP) in mussel gills, digestive gland and hemolymph samples, and to evaluate their possible utility in biomonitoring programs. Total antioxidant capacity (TAC) was measured by different methods: trolox equivalent antioxidant capacity (TEAC1 and TEAC2), cupric reducing antioxidant capacity (CUPRAC), and ferric reducing ability of plasma (FRAP)...
July 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29684538/molecular-cloning-characterization-and-expression-analysis-of-c-ebp-%C3%AE-%C3%AE-and-%C3%AE-in-adipose-related-tissues-and-adipocyte-of-duck-anas-platyrhynchos
#14
Jiamin Qiu, Wanxia Wang, Shenqiang Hu, Yushi Wang, Wenqiang Sun, Jiwei Hu, Xiang Gan, Jiwen Wang
CCAAT/enhancer binding protein α, β, δ (C/EBP α, β, δ) are essential transcriptional factors in regulating adipose development. However, information about their sequence characteristics and functions during adipocyte development still remains scarce in birds. In present study, we found that duck C/EBP α, β, δ differed in their phosphorylation sites and low complexity regions (LCRs) among their orthologs and paralogs. Phylogenetic analysis showed that C/EBP α, β, δ had different evolutionary patterns, and each of duck C/EBP α, β, δ was strikingly diverged from orthologs of other Aves...
July 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29655871/cloning-partial-sequencing-and-expression-analysis-of-the-neural-form-of-p450-aromatase-cyp19a1b-in-the-south-america-catfish-rhamdia-quelen
#15
Helena C Silva de Assis, Laia Navarro-Martín, Letícia S P Fernandes, Cibele C Cardoso, Daniela Parada Pavoni, Vance L Trudeau
Brain aromatase is a key enzyme exclusively expressed in fish radial glial cells that convert androgens into estrogens, thus controlling neuroendocrine functions and neurogenesis. As an important step in characterizing the neuroendocrine systems of Rhamdia quelen (jundiá), a partial cDNA sequence (1045 bp) of brain aromatase (cyp19a1b) was cloned and sequenced. At the nucleotide level the cDNA sequence was found to be 88% identical to cyp19a1b of two species of catfish, Ictalurus punctatus and Silurus meridionalis...
July 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29654886/role-of-abc-transporters-white-scarlet-and-brown-in-brown-planthopper-eye-pigmentation
#16
Yanyun Jiang, Xinda Lin
The brown planthopper ATP-binding cassette (ABC) proteins White (W), Scarlet (St) and Brown (Bw) belong to the ABC transporter superfamily and are responsible for the transportation of guanine and tryptophan precursors of eye pigments. In the present study, the brown planthopper White (NlW), S t(NlSt) and Bw (NlBw) genes were cloned, and subsequent phylogenetic analysis showed that these genes are clustered with their respective homologues, with a genetic relationship observed between NlW and its Bemisia tabaci homologue having the highest similarity...
July 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29649577/bioinformatics-characterization-of-a-cathepsin-b-transcript-from-the-giant-river-prawn-macrobrachium-rosenbergii-homology-modeling-and-expression-analysis-after-aeromonas-hydrophila-infection
#17
Saowalak Onming, Wilawan Thongda, Chao Li, Orathai Sawatdichaikul, Nichanun McMillan, Sirawut Klinbunga, Eric Peatman, Supawadee Poompuang
Cathepsin B is a lysosomal proteolytic enzyme that has been suggested to play a role in pathological processes of immune system. In this study, the full-length cDNA sequence of cathepsin B transcript in the giant river prawn Macrobrachium rosenbergii (MrCTSB) was obtained from 454 pyrosequencing of cDNAs from hepatopancreas and muscle. It was 1158 bp in length, containing an open reading frame (ORF) of 987 bp corresponding to 328 amino acids. The predicted molecular mass and pI of MrCTSB protein was 36...
July 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29580837/identification-and-characterization-of-two-distinct-sigma-class-glutathione-s-transferase-from-freshwater-bivalve-cristaria-plicata
#18
Zhenfang Li, Yuduan Cha, Baoqing Hu, Chungen Wen, Shaoqing Jian, Peipei Yi, Yang Gang
Glutathione-S-transferases (GSTs) are multifunctional phase II detoxification enzymes that catalyze the attachment of electrophilic substrates to glutathione, and play an important role in protecting organisms against the toxicity of reactive oxygen species. In this study, two distinct sigma-class GST (CpGSTσ1 and 2) cDNA sequences were cloned from freshwater bivalve Cristaria plicata. The full length cDNA of CpGSTσ1 and 2 was 826 bp and 1609 bp, which encoded 213 and 248 amino acid residues, respectively...
May 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29580836/differential-expression-of-two-piwil-orthologs-during-embryonic-and-gonadal-development-in-pufferfish-takifugu-fasciatus
#19
Xin Wen, Dan Wang, Xinru Li, Cheng Zhao, Tao Wang, Xiaoming Qian, Shaowu Yin
Piwil was an important regulator gene in germ cell division during gonadal development. Two Piwi-like genes, Piwil1 and Piwil2, were first cloned from T. fasciatus. The full-length cDNAs of Piwil1 and Piwil2 were of 2933 and 3394 bp, respectively. Piwil1 and Piwil2 possessed an open reading frame (ORF) of 2565 and 3138 bp, encoding 854 and 1045 amino acids, respectively. The tissue distribution analysis demonstrated that Piwil1 and Piwil2 were expressed at higher levels in gonad compared to other tissues (brain, liver, gill, etc...
May 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29567070/identification-of-duplicated-midkine-genes-and-their-functional-regulation-in-blunt-snout-bream-megalobrama-amblycephala
#20
Dan-Dan Guo, Guo-Dong Zheng, Shang-Ke Du, Bo Qin, Xia-Yun Jiang, Shu-Ming Zou
Midkine (Mdk) is a heparin-binding growth factor that is involved in regulating cell growth, differentiation and migration. Here, we report the isolation and characterization of duplicated mdk genes in blunt snout bream (Megalobrama amblycephala). The mdka and -b genes encode 146 aa and 147 aa peptides, respectively, sharing a sequence identity of 64%. During embryogenesis, mdka mRNA is detectable after 12 h post-fertilization (hpf) and mdkb mRNA can be detected after 8 hpf, about 4 h prior to mdka mRNA...
May 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
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