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Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology

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https://www.readbyqxmd.com/read/30236454/integrative-cytological-analysis-of-the-effects-of-ca-2-and-vitamin-d-3-on-extracellular-ca-2-flux-and-intracellular-ca-2-reserves-in-the-mantle-of-the-pearl-oyster-hyriopsis-cumingii-lea
#1
Wenjuan Li, Yuxiang Cao, Xingrong Xuan, Zhiyi Shi, Ye Zhang, Jiamin Liu, Zhiyi Bai, Yuanshuai Fu, Minglin Wu
To examine Ca2+ absorption and transportation in the freshwater pearl oyster, Hyriopsis cumingii Lea, we studied the effects of different levels of either extracellular Ca2+ or 1,25(OH)2 D3 on extracellular Ca2+ flux and intracellular Ca2+ concentrations in mantle cells using the non-invasive micro-test technique and laser scanning confocal microscopy. The inner and outer mantle (IM and OM) cells from mussels were cultured and then treated with different concentrations of Ca2+ and 1,25(OH)2 D3. Extracellular Ca2+ flux and intracellular Ca2+ reserves were analyzed...
September 17, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/30218714/comparison-of-steroidogenic-gene-expression-in-mummichog-fundulus-heteroclitus-testis-tissue-following-exposure-to-aromatizable-or-non-aromatizable-androgens
#2
Robert Rutherford, Andrea Lister, Deborah MacLatchy
Androgens are a recognized class of endocrine disrupting compounds with the ability to impact reproductive status in aquatic organisms. The current study utilized in vitro exposure of mummichog (Fundulus heteroclitus) testis tissue to either the aromatizable androgen 17α-methyltestosterone (MT) or the non-aromatizable androgen 5α-dihydrotestosterone (DHT) over the course of 24 h to determine if there were differential effects on steroidogenic gene expression. Testis tissue was exposed to androgen concentrations of 10-12  M, 10-9  M and 10-6  M for 6, 12, 18 or 24 h, after which a suite of steroidogenic genes, including steroidogenic acute regulatory protein, 3β-hydroxysteroid dehydrogenase (3βhsd) and cytochrome P450 17A1 (cyp17a1), were quantified using real-time polymerase chain reaction...
September 12, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/30201405/oxygen-dependent-distinct-expression-of-hif-1%C3%AE-gene-in-aerobic-and-anaerobic-tissues-of-the-amazon-oscar-astronotus-crassipinnis
#3
Heinrichs-Caldas Waldir, Campos Derek Felipe, Paula-Silva Maria Nazaré, Almeida-Val Vera Maria Fonsêca
The aquatic habitats of the Amazon basin present dramatic variation of oxygen level, and, to survive such changes, many aquatic animals developed biochemical and physiological adaptations. The advanced teleost Astronotus crassipinnis (Perciformes) is a fish tolerant to hypoxia and known to endure such naturally variable environments. Hypoxia-Inducible factor-1α (hif-1α) is among the most important and studied genes related to hypoxia-tolerance, maintaining regular cellular function and controlling anaerobic metabolism...
September 7, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/30196154/lipid-protein-interactions-in-mitochondrial-membranes-from-bivalve-mollusks-molecular-strategies-in-different-species
#4
Rosamaria Fiorini, Vittoria Ventrella, Fabiana Trombetti, Micaela Fabbri, Alessandra Pagliarani, Salvatore Nesci
The mitochondrial F1 FO -ATPase, the key enzyme in cell bioenergetics, apparently works in the same way in mollusks and in mammals. We previously pointed out a raft-like arrangement in mussel gill mitochondrial membranes, which apparently distinguishes bivalve mollusks from mammals. To explore the relationship between the microenvironmental features and the enzyme activity, the physico-chemical features of mitochondrial membranes and the F1 FO -ATPase activity temperature-dependence are here explored in the Manila clam (Ruditapes philippinarum)...
September 7, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/30195088/assessment-of-muscular-energy-metabolism-and-heat-shock-response-of-the-green-abalone-haliotis-fulgens-gastropoda-philipi-at-extreme-temperatures-combined-with-acute-hypoxia-and-hypercapnia
#5
Miguel A Tripp-Valdez, Christian Bock, Gisela Lannig, Nils Koschnick, Hans O Pörtner, Magnus Lucassen
The interaction between ocean warming, hypoxia and hypercapnia, suggested by climate projections, may push an organism earlier to the limits of its thermal tolerance window. In a previous study on juveniles of green abalone (Haliotis fulgens), combined exposure to hypoxia and hypercapnia during heat stress induced a lowered critical thermal maximum (CTmax ), indicated by constrained oxygen consumption, muscular spams and loss of attachment. Thus, the present study investigated the cell physiology in foot muscle of H...
September 6, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/30193833/developmental-dynamics-of-myogenesis-in-pacific-oyster-crassostrea-gigas
#6
Huijuan Li, Qi Li, Hong Yu, Shaojun Du
Pacific oyster (Crassostrea gigas) is a sessile bivalve living in the intertidal zone. It has become an attractive model for developmental studies due to its metamorphic transition from a mobile planktonic larvae to a sessile adults. To determine the effect of metamorphosis on muscle development in oyster larvae, we characterized myogenesis during larval development and metamorphosis by phalloidin staining which labels filamentous actin filaments. Our data revealed a dynamic pattern of myogenesis during embryonic and larval development...
September 5, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/30170023/genome-wide-identification-evolution-of-dna-methyltransferases-and-their-expression-during-gonadal-development-in-nile-tilapia
#7
Fei-Long Wang, Long-Xia Yan, Hong-Juan Shi, Xing-Yong Liu, Qiao-Yuan Zheng, Li-Na Sun, De-Shou Wang
DNA methyltransferases (dnmts) are responsible for DNA methylation and play important roles in organism development. In this study, seven dnmts genes (dnmt1, dnmt2, dnmt3aa, dnmt3ab, dnmt3ba, dnmt3bb.1, dnmt3bb.2) were identified in Nile tilapia. Comprehensive analyses of dnmts were performed using available genome databases from representative animal species. Phylogenetic analysis revealed that the dnmts family were highly conserved in teleosts. Based on transcriptome data from eight adult tilapia tissues, the dnmts were found to be dominantly expressed in the head kidney, testis and ovary...
August 28, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/30138681/myosin-va-from-eriocheir-sinensis-cdna-cloning-expression-and-involvement-in-growth-and-development
#8
Hao Li, Peng Li, Jie Yan, Wenli Xie, Peipei Wang, Haixia Wu, Kaiya Zhou
Myosin Va, a member of the myosin superfamily, has been widely identified associated with processes of cellular motility, which include neurotransmitter release and synaptic plasticity during neurodevelopment. However, the function of myosin Va in the growth and development of crustaceans has not yet been reported. In this study, a full-length cDNA of myosin Va (named as EsMyoVa) was cloned from the Chinese mitten crab, Eriocheir sinensis, and the expression patterns were detected in different tissues and larval developmental stages...
August 20, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/30118764/influence-of-dietary-lipid-levels-on-growth-nutrient-utilization-tissue-fatty-acid-composition-and-desaturase-gene-expression-in-silver-barb-puntius-gonionotous-fingerlings
#9
Madhusmita Nayak, Ashis Saha, Avinash Pradhan, Mrinal Samanta, Tapan K Mohanty, Shiba Shankar Giri
Silver barb (Puntius gonionotus) is considered as a promising medium-sized carp species for freshwater aquaculture in Asia. This study in silver barb was carried out to evaluate the effects of increasing dietary levels of lipid on growth, nutrient utilization, whole-body composition, tissue fatty acid composition and Δ6 fatty acyl desaturase (Δ6 fad) gene expression. Fish (11.3 ± 0.23 g of initial body weight) was fed for 60 days with five experimental diets: FO-0 (control feed); FO-30; FO-60; FO-90 and FO-120 containing 0, 30, 60, 90 and 120 g fish oil kg-1 diet, respectively...
August 15, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/30114527/characterization-of-midkine-in-tongue-sole-cynoglossus-semilaevis-and-its-role-on-the-germ-layer-genesis-in-zebrafish-danio-rerio
#10
Zhengrui Zhang, Zhenkui Qin
Midkine (Mdk) is a cytokine involved in controlling tissue repair and new tissue development, and regulating inflammation involved in several signaling pathway, such as the mitogen-activated protein kinase (MAPK) and phosphatidyl inositol 3-kinase (PI3K)/AKT pathways. But the role of Mdk in the development of Cynoglossus semilaevis is poorly understood. In this study, the Midkine of C. semilaevis (CsMdk) was cloned, and its spatiotemporal expression pattern and structural characteristics were analyzed. Furthermore, the essential genes related to nervous system development and germ layer formation marker gene were identified by qRT-PCR and in situ hybridization after overexpression the CsMdk via zebrafish model...
August 13, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/30114526/phoenixin-participated-in-regulation-of-food-intake-and-growth-in-spotted-scat-scatophagus-argus
#11
Mei Wang, Si-Ping Deng, Hua-Pu Chen, Dong-Neng Jiang, Chang-Xu Tian, Wei Yang, Tian-Li Wu, Chun-Hua Zhu, Yong Zhang, Guang-Li Li
Phoenixin (Pnx) is an endogenous peptide known to be involved in reproduction and food intake in rats, with two active isoforms, phoenixin-14 (Pnx-14) and phoenixin-20 (Pnx-20). However, little is known about the functions of Pnx in teleost. Here, pnx was cloned and was detected in all tissues of both male and female in spotted scat (Scatophagus argus), including growth axis, hypothalamus, pituitary, and liver. Real-time PCR analysis showed that pnx in the hypothalamus increased significantly after 2 d and 7 d fasting, while reduced significantly after re-feeding (P < 0...
August 13, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/30114525/expression-profile-and-localization-of-vitellogenin-mrna-and-protein-during-ovarian-development-in-turbot-scophthalmus-maximus
#12
Rui Xue, Xueying Wang, Shihong Xu, Yifan Liu, Chengcheng Feng, Chunyan Zhao, Qinghua Liu, Jun Li
Egg yolk generation is a common physiological process in oviparous animals. To understand oogenesis and reproductive capacity, it is necessary to characterize vitellogenins (Vtgs), which are the precursors of major egg yolk proteins (Yps). Therefore, to improve our understanding of the entire process of egg yolk generation in female turbot (Scophthalmus maximus), we obtained full-length cDNAs of vtg genes, examined gene expression in the female liver and ovary, and analyzed Vtg synthesis in the ovary. Three distinct complete polypeptide sequences were identified and designated as VtgAa, VtgAb, and VtgC, which confirmed the multiplicity of the vtg gene in turbot and showed that it follows a "three vtg model"...
August 13, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/30110659/molecular-cloning-and-functional-expression-of-the-5-ht-7-receptor-in-chinese-mitten-crab-eriocheir-sinensis
#13
Xiaozhen Yang, Genyong Huang, Minjie Xu, Cong Zhang, Yongxu Cheng
Serotonin (5-HT) regulates numerous physiological functions and processes, such as light adaptation, food intake and ovarian maturation, and plays the role through 5-HT receptors. To our knowledge, this is the first study to isolate and characterize the serotonin receptor 7 (5-HT7 receptor) cDNA encoded in Eriocheir sinensis, an economically important aquaculture species in China, by performing rapid-amplification of cDNA ends. The full-length of 5-HT7 receptor gene cDNA is 2328 bp and encodes a polypeptide with 590 amino acids that are highly homologous with other crustaceans 5-HT7 receptor genes...
August 12, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/30110658/role-of-adipokinetic-hormone-during-starvation-in-drosophila
#14
Michaela Mochanová, Aleš Tomčala, Zdeňka Svobodová, Dalibor Kodrík
The role of adipokinetic hormone (Drome-AKH) in maintaining the levels of basic nutrients, under starvation conditions, was studied using Drosophila melanogaster mutants with AKH deficiency (Akh1 ) and AKH abundance (EE-Akh). Our results showed lipids as the main energy reserve in Drosophila, and their physiological level and metabolism were shown to be under the control of AKH. AKH abundance in the body resulted in lower levels of triacylglycerols and diacylglycerols than in the controls, probably due to a more intensive metabolism; interestingly, there was a disproportional representation of fatty acids in triacylglycerols and diacylglycerols in Drosophila...
August 12, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/30107223/phosphoenolpyruvate-carboxykinase-cytosolic-and-mitochondrial-isoforms-are-expressed-and-active-during-hypoxia-in-the-white-shrimp-litopenaeus-vannamei
#15
Carlos A Reyes-Ramos, Alma B Peregrino-Uriarte, Keni Cota-Ruiz, Elisa M Valenzuela-Soto, Lilia Leyva-Carrillo, Gloria Yepiz-Plascencia
Hypoxic zones in marine environments are spreading around the world affecting the survival of many organisms. Marine animals have several strategies to respond to hypoxia, including the regulation of gluconeogenesis. Phosphoenolpyruvate carboxykinase (PEPCK) is a key regulatory enzyme of gluconeogenesis. The objective of this work was to study two isoforms of PEPCK, one mitochondrial (PEPKC-M) and one cytosolic (PEPCK-C), from the white shrimp Litopenaeus vannamei and the response to hypoxia. Both PEPCK isoforms are 72 kDa proteins and have 92% identity at the amino acid level...
August 11, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/30099196/effects-of-short-term-fasting-on-the-rhythmic-expression-of-core-circadian-clock-and-functional-genes-in-skeletal-muscle-of-goldfish-carassius-auratus
#16
Lingsheng Bao, Jianhua Wang, Shiyu Bin, Yulong Li, Wuying Chu, Jianshe Zhang
Molecular oscillators exist in peripheral tissues like pacemaker cells. Food intake is a dominant zeitgeber for peripheral clocks in vertebrates. Fasting is a physiological stress that elicits well-known metabolic adaptations, however, little is known about the effects of the rhythmic expression of clock components in skeletal muscle following short-term fasting in goldfish. Here, we characterized the molecular clock components and their daily transcription in COSINOR, and assessed the effect of 7-day fasting on the circadian patterns of the candidate genes expression in goldfish skeletal muscle...
August 10, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/30099195/expression-and-dna-methylation-analysis-of-cyp19a1a-in-chinese-sea-perch-lateolabrax-maculatus
#17
Xiaowu Chen, Yudong He, Zhipeng Wang, Jun Li
Cytochrome P450 aromatase (P450arom), which is encoded by cyp19a1a, can convert androgen to estrogen. Therefore, P450arom is important in gonadal differentiation and maintenance. In this study, we analyzed the expression and DNA methylation of cyp19a from Chinese sea perch Lateolabrax maculatus (sp. cyp19a1a). The sp. cyp19a1a gene consists of 9 exons, but only 3.5 kb, being smaller than the human cyp19a1a, as a result of small introns. The sp. cyp19a1a protein contains 518 amino acid residues and evolutionarily conserved domains and is clustered in the teleost subfamily on the phylogenetic tree...
August 9, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29966774/dietary-restriction-alters-the-fatbody-transcriptome-during-immune-responses-in-bombyx-mori
#18
Ye Pan, Peng Lü, Feifei Zhu, Chenjun Li, Yuanqing He, Keping Chen
Dietary restriction (DR) leads to extended lifespan in many species ranging from yeasts to mammal, and it can also affect the immune system to some extent. Herein, we investigated whether DR can enhance the immunity of Bombyx mori suffering from acute pathogenic microorganism infection. The results showed that DR could accelerate the melanisation reaction, delay the early death in silkworms, meanwhile Staphylococcus aureus (SA) load was lower in the early stage of infection. Moreover, more immune-related genes were identified to be down-regulated in the DR group infected with SA compared with the ad libitum - fed (AL) group infected with SA through mRNA deep sequencing (RNAseq) and quantitation PCR...
July 5, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29966773/host-plant-derived-allelochemicals-and-metal-components-are-associated-with-oxidative-predominance-and-antioxidant-plasticity-in-the-larval-tissues-of-silkworm-antheraea-mylitta-further-evidence-of-joint-effects-hypothesis
#19
Smaranika Sahu, Abinash Dutta, Dinesh Kumar Ray, Jyotsnarani Pradhan, Jagneshwar Dandapat
Tasar silkworm, Antheraea mylitta is a polyphagous insect that primarily feeds on Terminalia arjuna, Terminalia tomentosa and Shorea robusta. However, larval rearing on S. robusta results in poor performance for the reasons unexplored. Oxidative burden imposed by host plants is presumed to be a determining factor for larval fitness. With this hypothesis we have analyzed the foliar constituents of the respective host plants, the levels of oxidative stress and antioxidant protection in the larval tissues in response to their altered feeding on different host plants for different durations (2 and 10 days)...
June 30, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/30031885/identification-of-a-gene-encoding-microphthalmia-associated-transcription-factor-and-its-association-with-shell-color-in-the-clam-meretrix-petechialis
#20
Shujing Zhang, Hongxia Wang, Jiajia Yu, Fengjuan Jiang, Xin Yue, Baozhong Liu
The microphthalmia-associated transcription factor (MITF) is a master regulator of melanocyte development through the direct transcriptional control of related genes, e.g., the phenoloxidase gene. In this study, an MITF gene, MpMITF2, was identified in the clam Meretrix petechialis. The full-length cDNA of MpMITF2 was 2026 bp, and the molecular mass of the predicted protein was 42.6 kDa. A basic helix-loop-helix leucine zipper domain was detected in the deduced protein sequence, which can bind the E-box motif within the promoter of the downstream genes...
November 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
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