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Journal of Applied Genetics

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https://www.readbyqxmd.com/read/28191600/molecular-characterisation-of-tumour-necrosis-factor-alpha-and-its-potential-connection-with-lipoprotein-lipase-and-peroxisome-proliferator-activated-receptors-in-blunt-snout-bream-megalobrama-amblycephala
#1
Man Zhou, Hai-Feng Mi, Wen-Bin Liu, Ye-Yang Wu, Kai-Zhou Wang, Guang-Zhen Jiang
Tumour necrosis factor alpha (TNF-α) is one kind of cytokines which is related to inflammation and lipid metabolism. TNF-α cDNA was cloned from the liver of blunt snout bream (Megalobrama amblycephala) through real-time polymerase chain reaction (PCR) and rapid amplification of cDNA ends (RACE) methods. The full-length cDNA of TNF-α covered 1467 bp, with an open reading frame (ORF) of 723 bp, which encodes 240 amino acids. It possessed the TNF family signature IIIPDDGIYFVYSQ. After the lipopolysaccharide (LPS) challenge test, a graded tissue-specific expression pattern of TNF-α was observed and there was high expression abundance in the kidney, brain and liver...
February 13, 2017: Journal of Applied Genetics
https://www.readbyqxmd.com/read/28168627/disturbances-in-the-ploidy-level-in-the-gynogenetic-sterlet-acipenser-ruthenus
#2
D Fopp-Bayat, K Ocalewicz, M Kucinski, M Jankun, B Laczynska
Artificial mitotic gynogenesis, a chromosome set manipulation, is applied to provide the homozygous progeny with only maternal inheritance. Here, gynogenetic development was induced in the sterlet Acipenser ruthenus L. (Acipenseridae) by activation of the eggs originating from albino females with the UV-irradiated spermatozoa from wild-coloured males, followed by the heat shock applied to suppress the first mitotic division in the haploid zygotes. All experimentally obtained gynogenetic offspring possessed recessive albino coloration...
February 6, 2017: Journal of Applied Genetics
https://www.readbyqxmd.com/read/28101856/relationship-of-long-term-prognosis-to-mmp-and-timp-polymorphisms-in-patients-after-st-elevation-myocardial-infarction
#3
Monika Pavkova Goldbergova, Jiri Jarkovsky, Jolana Lipkova, Simona Littnerova, Martin Poloczek, Jindrich Spinar, Lenka Kubkova, Krystyna Kluz, Petr Kala, Jan Manousek, Anna Vasku, Jiri Parenica
The influence of polymorphisms in the large group of MMP and TIMP genes on clinical outcomes in patients after ST elevation myocardial infarction (STEMI) treated with primary PCI was analysed. In total, 550 consecutive Caucasian patients with STEMI were included in the present study, with a median of 32 months. We analysed 19 polymorphisms in the genes coding MMP and TIMP genes. The MMP-1 -519A/G and -422A/T polymorphisms are associated with combined endpoint after myocardial infarction. The hazard ratio for AT variant of MMP-1 -422A/T was 1...
January 18, 2017: Journal of Applied Genetics
https://www.readbyqxmd.com/read/28063128/improved-production-of-doubled-haploids-of-winter-and-spring-triticale-hybrids-via-combination-of-colchicine-treatments-on-anthers-and-regenerated-plants
#4
Aurelia Ślusarkiewicz-Jarzina, Hanna Pudelska, Jolanta Woźna, Tomasz Pniewski
Double haploids (DH), obtained during androgenesis in vitro or by genome diploidisation in regenerated haploids, are one type of basic materials used in triticale breeding programmes. The aim of this study was to improve DH production by a combination of colchicine treatment methods on a sample of five winter and five spring triticale hybrids. Colchicine was applied in vitro either in the C17 medium to induce embryo-like structures (ELS) or in the 190-2 medium for green plant (GP) development. Regenerants which remained haploid were immersed in a colchicine solution either when placed on the medium prior to transferring to soil or when growing in pots, followed by the application or absence of cooling...
January 6, 2017: Journal of Applied Genetics
https://www.readbyqxmd.com/read/28050760/identification-of-novel-candidate-genes-for-the-inverted-teat-defect-in-sows-using-a-genome-wide-marker-panel
#5
Helena Chalkias, Elisabeth Jonas, Lisa S Andersson, Magdalena Jacobson, Dirk Jan de Koning, Nils Lundeheim, Gabriella Lindgren
The number of functional teats is an important selection criterion in pig breeding. Inherited defects of the udder, such as the inverted teat, do have a considerable negative impact on the nursing ability of the sow. To investigate the genetic background of this defect and the number of functional teats in Swedish maternal lines, samples from 230 Yorkshire pigs were selected for genotyping using the PorcineSNP60K BeadChip (Illumina Inc.), each pig with at least one inverted teat was matched with one non-affected pig (fullsib or pairs with matching herd and gender)...
January 4, 2017: Journal of Applied Genetics
https://www.readbyqxmd.com/read/28032291/erratum-to-plasma-and-saliva-mir-21-expression-in-colorectal-cancer-patients
#6
A A Sazanov, E V Kiselyova, A A Zakharenko, M N Romanov, M I Zaraysky
No abstract text is available yet for this article.
December 28, 2016: Journal of Applied Genetics
https://www.readbyqxmd.com/read/27987181/association-of-snps-in-dopamine-and-serotonin-pathway-genes-and-their-interacting-genes-with-temperament-traits-in-charolais-cows
#7
E Garza-Brenner, A M Sifuentes-Rincón, R D Randel, F A Paredes-Sánchez, G M Parra-Bracamonte, W Arellano Vera, F A Rodríguez Almeida, A Segura Cabrera
Cattle temperament is a complex trait, and molecular studies aimed at defining this trait are scarce. We used an interaction networks approach to identify new genes (interacting genes) and to estimate their effects and those of 19 dopamine- and serotonin-related genes on the temperament traits of Charolais cattle. The genes proopiomelanocortin (POMC), neuropeptide Y (NPY), solute carrier family 18, member 2 (SLC18A2) and FBJ murine osteosarcoma viral oncogene homologue (FOSFBJ) were identified as new candidates...
December 16, 2016: Journal of Applied Genetics
https://www.readbyqxmd.com/read/27966062/diversity-of-mitochondrial-dna-in-three-arabian-horse-strains
#8
S Almarzook, M Reissmann, G A Brockmann
Arabian horse registries classify Arabian horses based on their dam lineages into five main strains. To test the maternal origin of Syrian Arabian horses, 192 horses representing the three major strains Saglawi, Kahlawi, and Hamdani were sequenced for 353 bp of their mitochondrial displacement loop (D-loop) region. Sequencing revealed 28 haplotypes comprising 38 sequence variations. The haplotype diversity values were 0.95, 0.91, and 0.90 in Kahlawi, Hamdani, and Saglawi strains, respectively. The pair-wise population differentiation estimates (Fst) between strains were low, ranging between 0...
December 14, 2016: Journal of Applied Genetics
https://www.readbyqxmd.com/read/27943120/polycyclic-aromatic-hydrocarbons-and-pah-related-dna-adducts
#9
REVIEW
Błaszczyk Ewa, Mielżyńska-Švach Danuta
Investigations on the impact of chemicals on the environment and human health have led to the development of an exposome concept. The exposome refers to the totality of exposures received by a person during life, including exposures to life-style factors, from the prenatal period to death. The exposure to genotoxic chemicals and their reactive metabolites can induce chemical modifications of DNA, such as, for example, DNA adducts, which have been extensively studied and which play a key role in chemically induced carcinogenesis...
December 12, 2016: Journal of Applied Genetics
https://www.readbyqxmd.com/read/27924448/milk-protein-polymorphisms-and-casein-haplotypes-in-butana-cattle
#10
A Said Ahmed, S Rahmatalla, R Bortfeldt, D Arends, M Reissmann, G A Brockmann
Butana is a Bos indicus dairy cattle breed that is well adapted to the local environment of Sudan. The breed has been gradually declining in number due to breed substitution. Therefore, conservation and improvement strategies are required to maintain this breed. The aim of the present study was to assess genetic variation that is characteristic for Butana cattle in the milk protein genes CSN1S1, CSN2, CSN1S2, CSN3, LALBA, and LGB. In a first step, genomic DNA of five unrelated individuals was comparatively sequenced across all exon and flanking sequences...
December 6, 2016: Journal of Applied Genetics
https://www.readbyqxmd.com/read/27924447/associations-between-polymorphisms-in-the-nicd-domain-of-bovine-notch1-gene-and-growth-traits-in-chinese-qinchuan-cattle
#11
Mei Liu, Chenge Zhang, Xinsheng Lai, Jing Xue, Xianyong Lan, Chuzhao Lei, Yutang Jia, Hong Chen
NOTCH1 is one of the four mammalian Notch receptors, which is involved in the Notch signaling pathway. Specifically, NOTCH1 promotes the proliferation of myogenic precursor cells, and the NICD domain of NOTCH1 can impair regeneration of skeletal muscles. However, similar research on the bovine NOTCH1 gene is lacking. In this study, we detected the polymorphisms of the bovine NOTCH1 gene in a total of 448 individuals from Chinese Qinchuan cattle with DNA pooling, forced PCR-RFLP, and DNA sequencing methods. Five novel SNPs were identified within the NICD domain, and eight haplotypes comprising combinations of these five SNPs were studied as well...
December 6, 2016: Journal of Applied Genetics
https://www.readbyqxmd.com/read/27917442/polymorphisms-in-ten-candidate-genes-are-associated-with-conformational-and-locomotive-traits-in-spanish-purebred-horses
#12
Natalia Sevane, Susana Dunner, Ana Boado, Javier Cañon
The Spanish Purebred horses, also known as Andalusian horses, compete to the highest standards in international dressage events. Gait and conformation could be used as early selection criteria to detect young horses with promising dressage ability. Although the genetic background of equine size variation has been recently uncovered, the genetic basis of horse conformational and locomotive traits is not known, hampered by the complex genetic architecture underlying quantitative traits and the lack of phenotypic data...
December 5, 2016: Journal of Applied Genetics
https://www.readbyqxmd.com/read/27910062/plasma-and-saliva-mir-21-expression-in-colorectal-cancer-patients
#13
A A Sazanov, E V Kiselyova, A A Zakharenko, M N Romanov, M I Zaraysky
MicroRNA-21 (miR-21) expression was quantified by real-time qRT-PCR in peripheral blood and saliva samples obtained from patients diagnosed with colorectal cancer (CRC) of varying degrees of malignancy and healthy volunteers. All patients had adenocarcinoma located in the distal colon at different stages. Significant differences were detected between the control group and the total experimental group of CRC patients (plasma, P = 0.0001; saliva, P = 5e-12). MiR-21 expression was also significantly different in certain subgroups of patients with CRC disease stages II-IV as compared to the control group...
December 2, 2016: Journal of Applied Genetics
https://www.readbyqxmd.com/read/27796943/modified-dna-polymerases-for-pcr-troubleshooting
#14
REVIEW
Marta Śpibida, Beata Krawczyk, Marcin Olszewski, Józef Kur
PCR has become an essential tool in biological science. However, researchers often encounter problems with difficult targets, inhibitors accompanying the samples, or PCR trouble related to DNA polymerase. Therefore, PCR optimization is necessary to obtain better results. One solution is using modified DNA polymerases with desirable properties for the experiments. In this article, PCR troubleshooting, depending on the DNA polymerase used, is shown. In addition, the reasons that might justify the need for modification of DNA polymerases, type of modifications, and links between modified DNA polymerases and PCR efficiency are described...
February 2017: Journal of Applied Genetics
https://www.readbyqxmd.com/read/27783333/relative-efficacy-of-nicotinamide-treatment-of-a-mouse-model-of-infantile-niemann-pick-c1-disease
#15
Craig A Marshall, Ivan A Borbon, Robert P Erickson
Nicotinamide delivered in drinking water at about 2 g/kg/day significantly prolonged survival and showed a suggestive improvement on memory in the Npc1 (nih) / Npc1 (nih) mouse model of infantile NPC1 disease. It is likely that this role is due to its function as a histone deacetylase (HDAC) inhibitor although another HDAC inhibitor, valproic acid, was without effect. Nicotinamide could also work by preventing/reversing oxidative stress.
February 2017: Journal of Applied Genetics
https://www.readbyqxmd.com/read/27629806/genomic-findings-in-patients-with-clinical-suspicion-of-22q11-2-deletion-syndrome
#16
Magdalena Koczkowska, Jolanta Wierzba, Robert Śmigiel, Maria Sąsiadek, Magdalena Cabała, Ryszard Ślężak, Mariola Iliszko, Iwona Kardaś, Janusz Limon, Beata S Lipska-Ziętkiewicz
Chromosome 22q11.2 deletion syndrome, one of the most common human genomic syndromes, has highly heterogeneous clinical presentation. Patients usually harbor a 1.5 to 3 Mb hemizygous deletion at chromosome 22q11.2, resulting in pathognomic TBX1, CRKL and/or MAPK1 haploinsufficiency. However, there are some individuals with clinical features resembling the syndrome who are eventually diagnosed with genomic disorders affecting other chromosomal regions. The objective of this study was to evaluate the additive value of high-resolution array-CGH testing in the cohort of 41 patients with clinical features of 22q11...
February 2017: Journal of Applied Genetics
https://www.readbyqxmd.com/read/27585670/limb-girdle-muscular-dystrophy-with-severe-heart-failure-overlapping-with-lipodystrophy-in-a-patient-with-lmna-mutation-p-ser334del
#17
Agnieszka Madej-Pilarczyk, Adam Niezgoda, Magdalena Janus, Romuald Wojnicz, Michał Marchel, Anna Fidziańska, Stefan Grajek, Irena Hausmanowa-Petrusewicz
Laminopathies, a group of heterogeneous disorders associated with lamin A/C gene (LMNA) mutations, encompass a wide spectrum of clinical phenotypes, which may present as separate disease or as overlapping syndromes. We describe a 35-year-old female in whom a novel sporadic heterozygous mutation c.1001_1003delGCC (p.Ser334del) of the LMNA gene was found. The patient presented with overlapping syndrome of heart failure secondary to dilated cardiomyopathy, limb-girdle dystrophy and partial lipodystrophy. Endomyocardial biopsy revealed strong up-regulation of HLA classes I and II antigens on microvessels and induction of the class I antigens on cardiomyocytes...
February 2017: Journal of Applied Genetics
https://www.readbyqxmd.com/read/27503092/qtls-for-earliness-and-yield-forming-traits-in-the-lubuski%C3%A2-%C3%A3-%C3%A2-camb-barley-ril-population-under-various-water-regimes
#18
Piotr Ogrodowicz, Tadeusz Adamski, Krzysztof Mikołajczak, Anetta Kuczyńska, Maria Surma, Paweł Krajewski, Aneta Sawikowska, Andrzej G Górny, Kornelia Gudyś, Iwona Szarejko, Justyna Guzy-Wróbelska, Karolina Krystkowiak
Drought has become more frequent in Central Europe causing large losses in cereal yields, especially of spring crops. The development of new varieties with increased tolerance to drought is a key tool for improvement of agricultural productivity. Material for the study consisted of 100 barley recombinant inbred lines (RILs) (LCam) derived from the cross between Syrian and European parents. The RILs and parental genotypes were examined in greenhouse experiments under well-watered and water-deficit conditions...
February 2017: Journal of Applied Genetics
https://www.readbyqxmd.com/read/27502940/interactions-between-glu-1-and-glu-3-loci-and-associations-of-selected-molecular-markers-with-quality-traits-in-winter-wheat-triticum-aestivum-l-dh-lines
#19
Karolina Krystkowiak, Monika Langner, Tadeusz Adamski, Bolesław P Salmanowicz, Zygmunt Kaczmarek, Paweł Krajewski, Maria Surma
The quality of wheat depends on a large complex of genes and environmental factors. The objective of this study was to identify quantitative trait loci controlling technological quality traits and their stability across environments, and to assess the impact of interaction between alleles at loci Glu-1 and Glu-3 on grain quality. DH lines were evaluated in field experiments over a period of 4 years, and genotyped using simple sequence repeat markers. Lines were analysed for grain yield (GY), thousand grain weight (TGW), protein content (PC), starch content (SC), wet gluten content (WG), Zeleny sedimentation value (ZS), alveograph parameter W (APW), hectolitre weight (HW), and grain hardness (GH)...
February 2017: Journal of Applied Genetics
https://www.readbyqxmd.com/read/27475083/genetic-correlation-estimates-between-beef-fatty-acid-profile-with-meat-and-carcass-traits-in-nellore-cattle-finished-in-feedlot
#20
Fabieli Loise Braga Feitosa, Bianca Ferreira Olivieri, Carolyn Aboujaoude, Angélica Simone Cravo Pereira, Marcos Vinicius Antunes de Lemos, Hermenegildo Lucas Justino Chiaia, Mariana Piatto Berton, Elisa Peripolli, Adrielle Matias Ferrinho, Lenise Freitas Mueller, Mônica Roberta Mazalli, Lucia Galvão de Albuquerque, Henrique Nunes de Oliveira, Humberto Tonhati, Rafael Espigolan, Rafael Lara Tonussi, Rafael Medeiros de Oliveira Silva, Daniel Gustavo Mansan Gordo, Ana Fabrícia Braga Magalhães, Ignacio Aguilar, Fernando Baldi
The objective of this study was to estimate the genetic-quantitative relationships between the beef fatty acid profile with the carcass and meat traits of Nellore cattle. A total of 1826 bulls finished in feedlot conditions and slaughtered at 24 months of age on average were used. The following carcass and meat traits were analysed: subcutaneous fat thickness (BF), shear force (SF) and total intramuscular fat (IMF). The fatty acid (FA) profile of the Longissimus thoracis samples was determined. Twenty-five FAs (18 individuals and seven groups of FAs) were selected due to their importance for human health...
February 2017: Journal of Applied Genetics
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