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Archives of Physiology and Biochemistry

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https://www.readbyqxmd.com/read/29020830/a-novel-biomarker-explaining-the-role-of-oxidative-stress-in-exercise-and-l-tyrosine-supplementation-thiol-disulphide-homeostasis
#1
Yildirim Kayacan, Hayrullah Yazar, Emin Can Kisa, Babak Elmi Ghojebeigloo
The present study aimed to evaluate the relationship between exercise and both l-tyrosine and oxidative stress using thiol/disulphide homeostasis via a novel biomarker in rats. Following the completion of the exercise and l-tyrosine protocol, serum total thiol, native thiol, and disulphide concentrations were determined using a novel automated measurement method. Compared with the control group, serum dynamic disulphide levels were significantly lower in the E group (116.75 ± 10.49; p < .05) and the highest in the LT group (151...
October 11, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/29020824/biomarkers-of-oxidative-stress-and-protein-protein-interaction-in-chronic-obstructive-pulmonary-disease
#2
Taru Aggarwal, Ridhima Wadhwa, Vishwajeet Rohil, Pawan Kumar Maurya
CONTENT: The increased oxidative stress in chronic obstructive pulmonary disease (COPD) patients is the result of increased inhaled oxidants, generated by various cells of the airways. OBJECTIVE: The investigation included measurements of malondiadehyde (MDA), uric acid, ascorbic acid, and matrix metalloproteinase-12 (MMP-12) in COPD patient. We also performed genetic analysis for protein-protein interaction (PPI) network. MATERIALS AND METHODS: The study was conducted on healthy subjects with normal lung function (NS, 14 subjects) and 28 patients (Global Initiative for Chronic Obstructive Lung Disease (Gold) 1 and Gold 2) with COPD...
October 11, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28952789/high-fat-diet-reduces-weight-gain-but-increases-other-cardio-metabolic-indices-in-offspring-of-normotensive-and-hypertensive-rats
#3
Oladele Ayobami Afolabi, Abdullateef Isiaka Alagbonsi, Roseline Toyosi Oyinloye, Hussein Mofomosara Salahdeen, Toyin Mohammed Salman, Lawrence Aderemi Olatunji
This study investigated the effect of post-natal consumption of high-fat diet (HFD) on cardio-metabolic indices in male offspring of hypertensive female rats. There were neither significant differences in body weight gain either in pups from normotensive or hypertensive dams that received normal diet during the post-weaning periods (except at 7th and 9th weeks), nor in both pup groups that received HFD. However, both pup groups that received HFD had reduced body weight gain when compared to their counterparts that received normal diet...
September 27, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28933207/correction-to-madhuri-and-naik-modulatory-effect-of-garcinol-in-streptozotocin-induced-diabetic-wistar-rats
#4
(no author information available yet)
No abstract text is available yet for this article.
September 21, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28920707/in-vitro-and-in-vivo-studies-of-allium-sativum-extract-against-deltamethrin-induced-oxidative-stress-in-rats-brain-and-kidney
#5
Marwa Ncir, Mongi Saoudi, Hanen Sellami, Fatma Rahmouni, Amina Lahyani, Fatma Makni Ayadi, Abdelfattah El Feki, Mohamed Salah Allagui
The present study investigated the in vitro and the in vivo antioxidant capacities of Allium sativum (garlic) extract against deltamethrin-induced oxidative damage in rat's brain and kidney. The in vitro result showed that highest extraction yield was achieved with methanol (20.08%). Among the tested extracts, the methanol extract exhibited the highest total phenolic, flavonoids contents and antioxidant activity. The in vivo results showed that deltamethrin treatment caused an increase of the acetylcholinesterase level (AChE) in brain and plasma, the brain and kidney conjugated dienes and lipid peroxidation (LPO) levels as compared to control group...
September 18, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28906145/protective-effect-of-rutin-against-brain-injury-induced-by-acrylamide-or-gamma-radiation-role-of-pi3k-akt-gsk-3%C3%AE-nrf-2-signalling-pathway
#6
Noura M Thabet, Enas M Moustafa
This study was designed to evaluate the effect of rutin on PI3K/AKT-signalling in case of acrylamide or γ-radiation-induced neurotoxicity. To induce brain damage, animals were received acrylamide (25 mg/kg b.wt./orally/day) or 5 Gy of γ-radiation exposure accompanied with an administration of rutin (200 mg/kg b.wt./orally/day). Our data revealed that, compared to acrylamide or γ-radiation, rutin activated PI3K/AKT/GSK-3β/NRF-2-pathway through increased protein levels of p-PI3K, p-AKT and p-GSK-3β and up-regulated the expression of NRF-2...
September 14, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28875714/effects-of-co-exposure-to-imidacloprid-and-gibberellic-acid-on-redox-status-kidney-variables-and-histopathology-in-adult-rats
#7
Bornia Lafi, Mariem Chaâbane, Awatef Elwej, Malek Grati, Kamel Jamoussi, Hela Mnif, Tahia Boudawara, Hanen Ketata Bouaziz, Najiba Zeghal
Data on the individual nephrotoxic effects of imidacloprid (IMI) and gibberellic acid (GA3) are scarce. Moreover, there is a lack of information about their combined effects on the renal tissue. Our study investigated the effects of IMI and GA3 separately or together on rats kidney. IMI (64 mg/kg bw) was given for 3 weeks by gavage either individually or in combination with GA3 (200 mg/L) via drinking water. IMI associated or no with GA3 increased the levels of kidney malondialdehyde, advanced oxidation protein products, protein carbonyls and metallothionein, plasma creatinine, urea, blood urea nitrogen and lactate dehydrogenase activity...
September 6, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28871825/the-effects-of-ex-vivo-ozone-treatment-on-human-erythrocyte-carbonic-anhydrase-enzyme
#8
Gülten Erken, Haydar Ali Erken, Cigdem Bilen, Nahit Gencer
Ozone autohemotherapy is used in the treatment of some diseases. Carbonic anhydrases (CAs, EC 4.2.1.1) are metalloenzymes and play a role in homeostatic mechanisms. The aim of this study was to investigate the effects of ozone on human red blood cell CA (hCA) enzyme activity. Blood samples were treated with different doses of ozone (10, 20, 30 µg/ml) and the erythrocyte total CA activities were determined. Also, purified hCAI and hCAII isozymes were treated with the same doses of ozone and the enzyme activities were measured...
September 5, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28868937/ameliorative-effect-of-nicotine-exposure-on-insulin-resistance-is-accompanied-by-decreased-cardiac-glycogen-synthase-kinase-3-and-plasminogen-activator-inhibitor-1-during-oral-oestrogen-progestin-therapy
#9
Olugbenga S Michael, Lawrence A Olatunji
CONTEXT: Cigarette smoking is considered to be a major risk factor for the development of diabetes and cardiovascular disease. Oestrogen-progestin combined oral contraceptive (COC) use has been associated with adverse cardiometabolic events. OBJECTIVE: We hypothesized that nicotine would ameliorate insulin resistance (IR) that is accompanied by decreased cardiac glycogen synthase kinase-3 (GSK-3) and plasminogen activator inhibitor-1 (PAI-1). METHODS: Female Wistar rats received (po) low-(0...
September 3, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28862046/glucose-metabolism-and-metabolic-flexibility-in-cultured-skeletal-muscle-cells-is-related-to-exercise-status-in-young-male-subjects
#10
Jenny Lund, Daniel S Tangen, Håvard Wiig, Hans K Stadheim, Siw A Helle, Jesper B Birk, Thorsten Ingemann-Hansen, Arild C Rustan, G Hege Thoresen, Jørgen F P Wojtaszewski, Eili T Kase, Jørgen Jensen
We hypothesised that skeletal muscles of healthy young people have a large variation in oxidative capacity and fibre-type composition, and aimed therefore to investigate glucose metabolism in biopsies and myotubes isolated from musculus vastus lateralis from healthy males with varying degrees of maximal oxygen uptake. Trained and intermediary trained subjects showed higher carbohydrate oxidation in vivo. Fibre-type distribution in biopsies and myotubes did not differ between groups. There was no correlation between fibre-type I expression in biopsies and myotubes...
September 1, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28857634/antiplatelets-and-profibrinolytic-activity-of-citrullus-colocynthis-in-control-and-high-fat-diet-induced-obese-rats-mechanisms-of-action
#11
Naif M Alhawiti
The current study aimed to investigate the effect of Citrullus colocynthis (C. colocynthis) hydro-alcoholic extract on blood haemostasis in control and high-fat diet (HFD) induced obese rats. In control rats, the extract significantly enhanced bleeding time and plasma levels of tPA and significantly decreased plasma levels PAI-1 and serum levels of thromboxane B2 leading to inhibition of platelets aggregation. In HFD induced obese rats, similar effects were seen and the extract was also able to reverse HFD induced increases in fibrinogen and VWF...
August 31, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28857629/high-intensity-interval-training-acutely-alters-plasma-adipokine-levels-in-young-overweight-obese-women
#12
Selma Arzu Vardar, Aziz Karaca, Sibel Güldiken, Orkide Palabıyık, Necdet Süt, Ahmet Muzaffer Demir
The aim of this study was to investigate the plasma adipokine responses to high-intensity interval training (HIT) in overweight/obese women. Twelve women (age 21.7 ± 3.8 years) completed a 19 days of HIT comprising six session of 4-6 repeats of a Wingate test (0.065 kg load/kg). Plasma adipokine levels were measured before exercise, and at 5 and 90 min after exercise on the first and the last training days. Adiponectin was higher at 5 min than 90 min post-exercise (11.7 ± 7.3 and 10.5 ± 5...
August 31, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28857622/supplementation-of-all-trans-retinoic-acid-ameliorates-ethanol-induced-endoplasmic-reticulum-stress
#13
Saritha S Nair, Syam Das S, Reshma P Nair, M Indira
CONTEXT: Molecular pathogenesis of chronic alcoholism is linked to increased endoplasmic reticulum stress. Ethanol is a competitive inhibitor of vitamin A metabolism and vitamin A supplementation aggravates existing liver problems. Hence, we probed into the impact of supplementation of all trans retinoic acid (ATRA), the active metabolite of vitamin A on ethanol-induced endoplasmic reticulcum stress. METHODS: Male Sprague-Dawley rats were divided into four groups - I: Control; II: Ethanol; III: ATRA; IV: ATRA + Ethanol...
August 31, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28853614/refeeding-after-caloric-restriction-reverses-altered-liver-glucose-release
#14
Rosângela F Garcia, Isabela R Mariano, Isabela C Stolarz, Maria Montserrat D Pedrosa
CONTEXT: Caloric restriction increases liver glucose release (LGR), but it is not known if this is a permanent condition. OBJECTIVE: To investigate if refeeding after caloric restriction reverses the high LGR. MATERIALS AND METHODS: Rats were organised in six-pups litters (GC); 12-pups litters with either 50% caloric restriction from 21 to 80 days of age (GR) or fed at will from 50 to 80 days of age (GRL). Liver perfusion was made at the age of 80 days...
August 30, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28850272/combined-effect-of-retinoic-acid-and-calcium-on-the-in-vitro-differentiation-of-human-adipose-derived-stem-cells-to-adipocytes
#15
Farjam Goudarzi, Arash Sarveazad, Maryam Mahmoudi, Adel Mohammadalipour, Reza Chahardoli, Obeid M Malekshah, Shiva Karimi Gorgani, Ali Akbar Saboor-Yaraghi
CONTEXT: It has been shown that adipogenesis can be modulated by factors such as all-trans retinoic acid (ATRA) and calcium. OBJECTIVE: To determine, the combined effect of ATRA and calcium on the differentiation of human adipose-derived stem cells (hADSCs). METHODS: Mesenchymal stem cells (MSCs) were differentiated into the adipocytes by 0.5 and 1 µM of ATRA and 5 and 10 mM calcium separately or in combination. After MTS assay the differentiation of MSCs to adipocyte was evaluated, Oil Red O staining, GLUT4 concentration and gene expression of PPARG2, adiponectin, and GLUT4 were measured by Real-Time PCR...
August 29, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28844165/really-does-temperature-reduction-and-norepinephrine-have-similar-effects-on-the-energy-metabolism-in-rat-brown-adipose-tissue
#16
B Sopeña, Z López-Ibarra, A J López-Farré, N de Las Heras, S Ballesteros, A González-Cantalapiedra, V Lahera, J J Zamorano-León
CONTEXT: Heat generation by brown adipose tissue (BAT) in response to temperature reduction seems to be entirely related to sympathetic nervous stimulation. OBJECTIVE: To analyse if temperature reduction and norepinephrine may differently affect the expression of proteins related to energy metabolism in BAT. MATERIALS AND METHODS: Isolated rats BAT was incubated with/without norepinephrine (10(-6) mol/L, 24 h at 32 °C and 37 °C). RESULTS: In BAT, 32 °C increased the protein expression levels of carnitine palmitoyltransferase-I and -II, mitochondrial uncoupling protein-1 (UCP-1) and the expression and activity of lactate dehydrogenase...
August 26, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28835131/il-1%C3%AE-and-tnf%C3%AE-inhibit-gpr120-ffar4-and-stimulate-gpr84-ex33-and-gpr41-ffar3-fatty-acid-receptor-expression-in-human-adipocytes-implications-for-the-anti-inflammatory-action-of-n-3-fatty-acids
#17
Laura Muredda, Małgorzata A Kępczyńska, Mohamed S Zaibi, Suliman Y Alomar, Paul Trayhurn
Regulation of the expression of GPCR fatty acid receptor genes has been examined in human adipocytes differentiated in culture. TNFα and IL-1β induced a marked reduction in GPR120 expression, mRNA level falling 17-fold at 24 h in adipocytes incubated with TNFα. In contrast, GPR84 mRNA was dramatically increased by these cytokines (>500-fold for IL-1β at 4 h); GPR41 expression was also stimulated. Rosiglitazone did not affect GPR84 expression, but GPR120 and GPR41 expression increased. Dexamethasone, insulin, linoleic and docosahexaenoic acids (DHA), and TUG891 (GPR120 agonist) had little effect on GPR120 and GPR84 expression...
August 24, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28835129/preclinical-renal-chemo-protective-potential-of-prunus-amygdalus-batsch-seed-coat-via-alteration-of-multiple-molecular-pathways
#18
Preeti Pandey, Prakash Chandra Bhatt, Mahfoozur Rahman, Dinesh Kumar Patel, Firoz Anwar, Fahad Al-Abbasi, Amita Verma, Vikas Kumar
Prunus amygdalus Batsch (almond) is a classical nutritive traditional Indian medicine. Along with nutritive with anti-oxidant properties, it is, clinically, used in the treatment of various diseases with underlying anti-oxidant mechanism. This study is an effort to scrutinise the renal protective effect of P. amygdalus Batsch or green almond (GA) seed coat extract and its underlying mechanism in animal model of Ferric nitrilotriacetate (Fe-NTA) induced renal cell carcinoma (RCC). RCC was induced in Swiss Albino Wistar rats by intraperitoneal injection of Fe-NTA...
August 24, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28817314/role-of-geraniol-against-lead-acetate-mediated-hepatic-damage-and-their-interaction-with-liver-carboxylesterase-activity-in-rats
#19
Ahmet Ozkaya, Zafer Sahin, Muslum Kuzu, Yavuz Selim Saglam, Mustafa Ozkaraca, Mirac Uckun, Ertan Yologlu, Veysel Comakli, Ramazan Demirdag, Semra Yologlu
In this study, the effect of geraniol (50 mg/kg for 30 d), a natural antioxidant and repellent/antifeedant monoterpene, in a rat model of lead acetate-induced (500 ppm for 30 d) liver damage was evaluated. Hepatic malondialdehyde increased in the lead acetate group. Reduced glutathione unchanged, but glutathione S-transferase, glutathione reductase, as well as carboxylesterase activities decreased in geraniol, lead acetate and geraniol + lead acetate groups. 8-OhDG immunoreactivity, mononuclear cell infiltrations and hepatic lead concentration were lower in the geraniol + lead acetate group than the lead acetate group...
August 17, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28796539/carbonic-anhydrase-i-ii-autoantibodies-and-oxidative-status-in-long-term-follow-up-of-patients-with-crimean-congo-haemorrhagic-fever
#20
Hülya Yilmaz, Uğur Kostakoğlu, Selim Demir, Firdevs Aksoy, Ahmet Menteşe, Süleyman Caner Karahan, İftihar Köksal, Ahmet Alver, Gürdal Yilmaz
CONTEXT: Crimean-Congo haemorrhagic fever (CCHF) is a life-threatening acute febrile haemorrhagic disease. OBJECTIVE: This study was to measure levels of the oxidative stress biomarkers malondialdehyde (MDA), total oxidant status (TOS), total antioxidant status (TAS) and oxidative stress index (OSI) and of CA I-II autoantibodies as biomarkers for autoimmunity and course of disease in patients with CCHF. METHODS: Seventy CCHF patients and 39 healthy control volunteers were included in the study...
August 10, 2017: Archives of Physiology and Biochemistry
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