journal
MENU ▼
Read by QxMD icon Read
search

Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease

journal
https://www.readbyqxmd.com/read/28447869/mitigation-of-discordant-rifampicin-susceptibility-results-obtained-by-xpert-mycobacterium-tuberculosis-rifampicin-and-mycobacterium-growth-indicator-tube
#1
Elmira Gurbanova, Rafail Mehdiyev, Kai Blondal, Rasim Tahirli, Fuad Mirzayev, Doris Hillemann, Asker Ismayilov, Alan Altraja
BACKGROUND: Simultaneous use of genotypic and phenotypic diagnostic tools for detection of rifampicin (RIF) susceptibility may yield discrepant results. OBJECTIVE: To measure the discordance between the RIF-susceptibility results by Xpert MTB/RIF and Mycobacterium Growth Indicator Tube (MGIT), to evaluate if application of both tests to the same sample affects the discrepancy, and to evaluate treatment outcome in patients with the discordant strains. DESIGN: Sputa from patients with tuberculosis managed in the penitentiary system of Azerbaijan during 2011-2015 were examined for RIF susceptibility using Xpert MTB/RIF and MGIT...
April 27, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28445109/distinct-prevalence-of-drug-resistant-tuberculosis-in-gansu-china-a-retrospective-study-on-drug-susceptibility-profiles-between-2010-and-2014
#2
XingFang Li, Lei Wang, Yanling Tan, Jun Hou, Jianjun Ma
Limited knowledge is available on regional distribution of antituberculosis (TB) drug resistance and on province-specific time-trends in TB drug susceptibility in China. To obtain the latest information about the dynamics of drug-resistant TB in Gansu province, we conducted a retrospective study and analyzed data on drug resistance among new TB cases diagnosed between June 2010 and May 2014 in Gansu, China. The data were collected from the only TB surveillance and treatment hospital in Gansu and, therefore, represented the epidemiology of TB in the province...
April 26, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28445079/multiple-antimicrobial-resistance-and-novel-point-mutation-in-fluoroquinolone-resistant-escherichia-coli-isolates-from-mangalore-india
#3
Santhosh Kogaluru Shivakumaraswamy, Deekshit Vijaya Kumar, Venugopal Moleyuru Nagarajappa, Iddya Karunasagar, Indrani Karunasagar
Fluoroquinolone resistance in bacteria is usually associated with mutations in the topoisomerase regions. We report a novel point mutation in fluoroquinolone-resistant Escherichia coli strains. E. coli isolated from the environment in and around Mangalore, India, were examined for their antimicrobial resistance profile to 12 antibiotics and for the antibiotic resistance genes by polymerase chain reaction. Of the 67 E. coli isolated, 24 (35.8%) were sensitive to all antibiotics and 43 (64.2%) showed resistance to at least one of the 12 antibiotics used in the study...
April 26, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28437233/in-vitro-activity-of-various-antibiotics-in-combination-with-tigecycline-against-acinetobacter-baumannii-a-systematic-review-and-meta-analysis
#4
Jian Li, Xianghai Yang, Lidan Chen, Xinran Duan, Zhihui Jiang
Given that tigecycline-based combination therapy is recognized as a valuable option for the treatment of tigecycline-resistant Acinetobacter baumannii, we conducted this systematic review and meta-analysis to assess the overall evidence of its effectiveness. The synergy rate was defined as the primary outcome that was calculated separately for time-kill, Etest, and checkerboard microdilution methods. The secondary outcomes were bactericidal activity and the efficacy of combination treatment on the development of resistance...
April 24, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28414572/molecular-characterization-of-fluoroquinolone-resistant-haemophilus-parainfluenzae-clinical-isolates-in-spain
#5
Inmaculada López-Hernández, Jesús Machuca, Paula Díaz de Alba, Carolina Sarvisé, Frederic Gómez, José Manuel Rodríguez-Martínez, Alvaro Pascual
OBJECTIVE: The objective was to characterize a group of clinical isolates of fluoroquinolone-resistant Haemophilus parainfluenzae collected in Northern Spain (March-December 2014). METHODS: Twelve clinical isolates of H. parainfluenzae were studied by performing antimicrobial susceptibility testing and PCR amplification and nucleotide sequencing of the QRDR (quinolone resistance-determining region) of gyrA, parC, gyrB, and parE genes. Screening for plasmid-mediated quinolone resistance (PMQR) was also studied...
April 17, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28402182/analysis-of-point-mutations-in-the-pbp2x-pbp2b-and-pbp1a-genes-of-streptococcus-agalactiae-and-their-relation-with-a-reduced-susceptibility-to-cephalosporins
#6
Giorgio Piccinelli, Giuseppe Carlentini, Franco Gargiulo, Arnaldo Caruso, Maria Antonia De Francesco
The emergence of group B Streptococcus (GBS) isolates with reduced penicillin susceptibility (PRGBS) and their tendency to be nonsusceptible to fluoroquinolones prompted us to analyze the possible presence of amino acid mutations in penicillin-binding proteins (PBPs) (PBP2X, PBP1A, and PBP2B) from a collection of fluoroquinolone-resistant GBS isolates. We analyzed 21 GBS isolates resistant to levofloxacin. Sequence analysis of genes for PBPs was performed. The minimal inhibitory concentrations (MICs) for penicillin, ceftibuten, cefaclor, cefixime, cefotaxime, and ceftizoxime were performed by the Etest method and by broth microdilution method...
April 12, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28380311/spread-of-ctx-type-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-isolates-of-epidemic-clone-b2-o25-st131-among-dogs-and-cats-in-japan
#7
Kumiko Kawamura, Tae Sugawara, Nao Matsuo, Kengo Hayashi, Chihiro Norizuki, Kiyoko Tamai, Takaaki Kondo, Yoshichika Arakawa
This study was performed to investigate the carriage rates of CTX-M-type extended-spectrum β-lactamase (ESBL)-producing Escherichia coli among ill companion animals in Japan. Among the 178 nonrepetitive E. coli isolates, including 131 from dogs and 47 from cats, collected between September and November 2015, 42 (23.6%) isolates from 29 dogs and 13 cats were identified as ESBL producers. The antimicrobial susceptibility, O serotype, phylogenetic group, β-lactamase genotype, plasmid replicon type, and sequence type (ST) of each isolate were analyzed...
April 5, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28375698/extended-spectrum-%C3%AE-lactamase-producing-klebsiella-pneumoniae-from-pharmaceutical-wastewaters-in-south-western-nigeria
#8
Avemaria Obasi, SimonCyril Nwachukwu, Esther Ugoji, Christian Kohler, André Göhler, Veronica Balau, Yvonne Pfeifer, Ivo Steinmetz
Emergence and spread of Klebsiella pneumoniae isolates producing extended-spectrum β-lactamases (ESBLs) present a major threat to public health. In this study, we characterized β-lactam-resistant K. pneumoniae isolates from six wastewater samples obtained from two pharmaceutical industries located in Lagos and Ogun States, Nigeria. Bacteria were isolated by using MacConkey agar; species identification and antibacterial susceptibility testing were performed by Vitek 2. Etest was used for ESBL phenotype confirmation...
April 4, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28358617/bioactivity-of-variant-molecular-weight-chitosan-against-drug-resistant-bacteria-isolated-from-human-wounds
#9
Ijaz Bano, Muhammad Arshad, Muhammad Afzal Ghauri, Tariq Yasin, Muhammad Younus
Chitosan available from crab shells is usually of high molecular weight which may result in reduced efficiency for its antibacterial activity. One of the techniques for improving chitosan antibacterial efficiency is reducing its molecular weight. The irradiation of chitosan by gamma radiations is considered to be one of the most effective and widely used methods for improving its antibacterial activity. Chitosan obtained from crab shells was irradiated with gamma radiations at different doses, and effects on chitosan were analyzed by molecular weight determination and Fourier Transform Infrared spectroscopy...
March 30, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28358616/nucleotide-sequence-variations-in-autolysis-genes-of-st59-methicillin-resistant-staphylococcus-aureus-isolates
#10
Lee-Chung Lin, Jwu-Ching Shu, Shih-Cheng Chang, Mao-Cheng Ge, Tsui-Ping Liu, Chien-Wei Chen, Jang-Jih Lu
Biofilm formation is a virulence factor of bacteria. The goal of this study was to understand the mechanisms of biofilm formation by methicillin-resistant Staphylococcus aureus (MRSA). Whole-genome sequencing of eight MRSA strains was performed to identify sequence variations in genes related to biofilm formation. Thirty-one genes involved in MRSA biofilm formation were analyzed and 11 amino acid sequence variations in four genes related to autolysis were found. These variations include E121D and H387 N in ArlS; Q117K, T424S, K428T, A509S, V752E, A754V, and T771A in Atl; T184K in CidC; and D251N in CidR...
March 30, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28353392/epidemiology-and-molecular-basis-of-resistance-to-fluconazole-among-clinical-candida-parapsilosis-isolates-in-kuwait
#11
Mohammad Asadzadeh, Suhail Ahmad, Noura Al-Sweih, Ziauddin Khan
Fluconazole resistance among clinical Candida parapsilosis isolates is an emerging problem in many countries, including Kuwait. Resistance to fluconazole is mediated by amino acid substitutions in ERG11 and/or by overexpression of efflux pumps MDR1 and CDR1. Clinical C. parapsilosis sensu stricto isolates (n = 442) were tested for susceptibility to fluconazole by Etest, Vitek II, and broth microdilution methods. ERG11 was analyzed from fluconazole-resistant, fluconazole-susceptible dose-dependent, and selected fluconazole-susceptible isolates...
March 29, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28346849/clostridium-perfringens-type-a-isolates-of-animal-origin-with-decreased-susceptibility-to-metronidazole-show-extensive-genetic-diversity
#12
Sergio Álvarez-Pérez, José L Blanco, Marta E García
Metronidazole is a first-line drug for the treatment of human infections caused by anaerobic bacteria. Although most Clostridium perfringens isolates are susceptible to metronidazole, a number of strains with decreased susceptibility have been obtained in clinical and environmental surveys. However, little is known yet about the toxinotype and genetic diversity of metronidazole-resistant C. perfringens strains. We tested for susceptibility to metronidazole and assessed the toxigenic status and amplified fragment length polymorphism (AFLP) diversity of a collection of 80 C...
March 27, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28304216/characterizing-the-multidrug-resistance-of-non-o157-shiga-toxin-producing-escherichia-coli-isolates-from-cattle-farms-and-abattoirs
#13
Carrie-Ann Kennedy, Séamus Fanning, Maria Karczmarczyk, Brian Byrne, Áine Monaghan, Declan Bolton, Torres Sweeney
Non-O157 Shiga toxin-producing Escherichia coli (STECs) are not as well characterized as O157 STEC cases, despite their similar prevalence in many countries. Hence, the objective of this study was to investigate the phenotypic and genotypic basis of multidrug resistance (MDR) in non-O157 STEC farm- and abattoir-sourced isolates and assess the potential dissemination of these MDR profiles in vitro. Susceptibility testing to 20 antimicrobials was performed on 146 non-O157 STECs isolated from farm and abattoir environments...
March 17, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28304213/characterization-of-the-genetic-environment-of-the-blaveb-4-gene-associated-with-a-transposable-region-in-a-proteus-mirabilis-clinical-isolate
#14
Paula Espinal, Elisenda Miró, Laia Ramoneda, Manel Flores, Alba Rivera, Pere Coll, Ferran Navarro
Proteus mirabilis is the second most common cause of urinary tract infections and is also an important cause of nosocomial infections. TEM-type and CTX-M-type extended-spectrum β-lactamases (ESBLs) are the most widely distributed in this bacterial species, but minor ESBLs such as the VEB-type have also been identified. The aim of this study was to analyze the genetic environment of the blaVEB-4 gene found in a P. mirabilis clinical isolate recovered in Spain. P. mirabilis N2231 showed resistance to penicillins, cephalosporins, and aminoglycosides, remaining susceptible to imipenem, cefoxitin, β-lactamases inhibitors, and quinolones...
March 17, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28287910/transferability-of-the-mcr-1-colistin-resistance-gene
#15
Valérie Dénervaud Tendon, Laurent Poirel, Patrice Nordmann
No abstract text is available yet for this article.
March 13, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28287903/cytotoxic-effect-associated-with-overexpression-of-qnr-proteins-in-escherichia-coli
#16
Jesús Machuca, Paula Diaz de Alba, Esther Recacha, Álvaro Pascual, José Manuel Rodriguez-Martinez
OBJECTIVE: The objective was to evaluate the cytotoxic effect associated with overexpression of multiple Qnr-like plasmid-mediated quinolone resistance (PMQR) mechanisms in Escherichia coli. METHODS: Coding regions of different PMQR genes (qnrA1, qnrB1, qnrC, qnrD1, qnrS1, and qepA2) and efsqnr were cloned into pET29a(+) vector and overexpressed in E. coli BL21. E. coli BL21 with and without an empty pET29a(+) vector were used as controls. The cytotoxic effect associated with PMQR mechanism overexpression was determined by transmission electron microscopy and viability assays...
March 13, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28437232/epidemiological-physiological-and-molecular-characteristics-of-a-brazilian-collection-of-carbapenem-resistant-acinetobacter-baumannii-and-pseudomonas-aeruginosa
#17
Vanessa Cordeiro Dias, Juliana Alves Resende, André Netto Bastos, Lucas Quinet De Andrade Bastos, Victor Quinet De Andrade Bastos, Ricardo Villela Bastos, Cláudio Galuppo Diniz, Vânia Lúcia Da Silva
Nonfermenting Gram-negative bacteria such as Pseudomonas aeruginosa and Acinetobacter baumannii are widespread in the environment and are increasingly associated with nosocomial infections, often associated with multidrug-resistance phenotypes. This study aimed to evaluate epidemiological, physiological, and molecular characteristics of carbapenem resistance in P. aeruginosa and A. baumannii. In total, 63 nonreplicated strains (44 A. baumannii and 19 P. aeruginosa) were isolated from hospitalized patients. Antimicrobial resistance patterns, biocide tolerance, oxidative stress, hemolytic activity, and biofilm formation were assessed...
February 24, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28437231/capsular-polysaccharide-types-and-virulence-related-traits-of-epidemic-kpc-producing-klebsiella-pneumoniae-isolates-in-a-chinese-university-hospital
#18
Yang Liu, Pan-Pan Liu, Lian-Hui Wang, Dan-Dan Wei, La-Gen Wan, Wei Zhang
Klebsiella pneumoniae is an important human pathogen associated with a variety of diseases and the prevalence of blaKPC carrying K. pneumoniae (KPC-Kp) is rapidly increasing. Capsule is an important virulence factor in K. pneumoniae. In this study, we determined to first systematically characterize capsular polysaccharide (CPS) and virulence traits in KPC-Kp strains. A total of 56 KPC-Kp isolates were recovered from clinical samples in a Chinese hospital, which were assigned to clonal lineages by multilocus sequence typing (MLST)...
February 24, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28437230/high-diversity-of-antimicrobial-resistance-genes-class-1-integrons-and-genotypes-of-multidrug-resistant-escherichia-coli-in-beef-carcasses
#19
Chih-Ming Chen, Se-Chin Ke, Chia-Ru Li, Ying-Chen Wu, Ter-Hsin Chen, Chih-Ho Lai, Xin-Xia Wu, Lii-Tzu Wu
Multidrug-resistant Escherichia coli can contaminate food meat during processing and cause human infection. Phenotypic and genotypic characterization of the antimicrobial resistance were conducted for 45 multidrug-resistant E. coli isolates from 208 samples of beef carcasses. The mechanisms of resistance were evaluated using polymerase chain reaction and sequencing methods, and the clonal relationship among isolates was evaluated using multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE)...
February 23, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28437229/quinolone-resistance-determinants-of-clinical-salmonella-enteritidis-in-thailand
#20
Fuangfa Utrarachkij, Chie Nakajima, Ruchirada Changkwanyeun, Kanokrat Siripanichgon, Siriporn Kongsoi, Srirat Pornruangwong, Kanjana Changkaew, Risa Tsunoda, Yutaka Tamura, Orasa Suthienkul, Yasuhiko Suzuki
Salmonella Enteritidis has emerged as a global concern regarding quinolone resistance and invasive potential. Although quinolone-resistant S. Enteritidis has been observed with high frequency in Thailand, information on the mechanism of resistance acquisition is limited. To elucidate the mechanism, a total of 158 clinical isolates of nalidixic acid (NAL)-resistant S. Enteritidis were collected throughout Thailand, and the quinolone resistance determinants were investigated in the context of resistance levels to NAL, norfloxacin (NOR), and ciprofloxacin (CIP)...
February 23, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
journal
journal
32292
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"