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International Journal of Biochemistry & Cell Biology

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https://www.readbyqxmd.com/read/30016696/characterization-of-a-novel-endogenous-cysteine-proteinase-inhibitor-trichocystatin-3-tc-3-localized-on-the-surface-of-trichomonas-vaginalis
#1
Diana Belén Sánchez-Rodríguez, Jaime Ortega-López, Rosa Elena Cárdenas-Guerra, Gerardo Reséndiz-Cardiel, Bibiana Chávez-Munguía, Anel Lagunes-Guillen, Rossana Arroyo
Trichomonas vaginalis is a flagellated protist responsible for human trichomoniasis. T. vaginalis has three genes encoding for endogenous cysteine proteinase (CP) inhibitors, known as trichocystatin-1 through trichocystatin-3 (TC-1, TC-2, and TC-3). These inhibitors belong to the cystatin family. In this study, we characterized trichocystatin-3 (TC-3), an endogenous cysteine proteinase (CP) inhibitor of T. vaginalis. TC-3 possesses a signal peptide in the N-terminus and two putative glycosylation sites (typical of family 2, cystatins) but lacks the PW motif and cysteine residues (typical of family 1, stefins)...
July 14, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/30016695/molecular-and-in-silico-analyses-validates-pathogenicity-of-homozygous-mutations-in-the-npr2-gene-underlying-variable-phenotypes-of-acromesomelic-dysplasia-type-maroteaux
#2
Irfanullah, Amir Zeb, Naila Shinwari, Khadim Shah, Syed Zohaib Tayyab Gilani, Saadullah Khan, Keun Woo Lee, Syed Irfan Raza, Shabir Hussain, Khurram Liaqat, Wasim Ahmad
Homozygous and/or heterozygous loss of function mutations in the natriuretic peptide receptor B (NPR2) have been reported in causing acromesomelic dysplasia, type Maroteaux with variable clinical features and idiopathic short stature with nonspecific skeletal deformities. On the other hand, gain of function mutations in the same gene result in overgrowth disorder suggesting that NPR2 and its ligand, natriuretic peptide precursor C (CNP), are the key players of endochondral bone growth. However, the precise mechanism behind phenotypic variability of the NPR2 mutations is not fully understood so far...
July 14, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/30010013/roles-of-microrna-in-prostate-cancer-cell-metabolism
#3
Khanmi Kasomva, Arnab Sen, Michael Gabriel Paulraj, Stephen Sailo, Vandana Raphael, Kekungu-U Puro, Shiwoto Ruth Assumi, Savarimuthu Ignacimuthu
MicroRNAs are non-coding RNA which functions as regulators of genes expression. MicroRNAs have shown their biological functions in cell proliferation, cell cycle, cell metabolism, apoptosis, invasion and metastasis. Cancer cells have the ability to grow in the absence of growth factors by increased metabolic activity. MicroRNAs regulate cell metabolic processes by targeting the key enzymes or transporters and change the metabolic activities by interfering with oncogenes/tumor suppressors, hypoxia, signalling pathways and cell adhesion...
July 12, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/30010012/dnmt-and-hdac-inhibitors-together-abrogate-endotoxemia-mediated-macrophage-death-by-stat3-jmjd3-signaling
#4
Saheli Samanta, Zhigang Zhou, Sheeja Rajasingh, Arunima Panda, Venkatesh Sampath, Johnson Rajasingh
Acute lung injury (ALI) is a common complication of sepsis that often leads to fatal lung disease without effective therapies. It is known that bone marrow derived macrophages are important in resolving the inflammation and maintaining tissue homeostasis. Here, we hypothesize that treatment in combination of DNA methyl transferase inhibitor (DNMTi) 5-Aza 2-deoxycytidine (Aza) and histone deacetylase inhibitor (HDACi) Trichostatin A (TSA) mitigates the inflammation induced pyroptosis and apoptosis during endotoxemia induced ALI...
July 12, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29997057/mitochondrial-copper-homeostasis-and-its-derailment-in-wilson-disease
#5
Hans Zischka, Claudia Einer
In mitochondria, copper is a Janus-faced trace element. While it is the essential cofactor of the mitochondrial cytochrome c oxidase, a surplus of copper can be highly detrimental to these organelles. On the one hand, mitochondria are strictly dependent on adequate copper supply for proper respiratory function, and the molecular mechanisms for metalation of the cytochrome c oxidase have been largely characterized. On the other hand, copper overload impairs mitochondria and uncertainties exist concerning the molecular mechanisms for mitochondrial metal uptake, storage and release...
July 8, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29960079/cousins-at-work-how-combining-medical-with-optical-imaging-enhances-in-vivo-cell-tracking
#6
Alessia Volpe, Ewelina Kurtys, Gilbert O Fruhwirth
Microscopy and medical imaging are related in their exploitation of electromagnetic waves, but were developed to satisfy differing needs, namely to observe small objects or to look inside subjects/objects, respectively. Together, these techniques can help elucidate complex biological processes and better understand health and disease. A current major challenge is to delineate mechanisms governing cell migration and tissue invasion in organismal development, the immune system and in human diseases such as cancer where the spatiotemporal tracking of small cell numbers in live animal models is extremely challenging...
June 27, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29953965/microrna-17-inhibition-overcomes-chemoresistance-and-suppresses-epithelial-mesenchymal-transition-through-a-dedd-dependent-mechanism-in-gastric-cancer
#7
Dong-Mei Wu, Xiao-Wu Hong, Ling-Ling Wang, Xia-Feng Cui, Jun Lu, Gui-Quan Chen, Yuan-Lin Zheng
MicroRNAs (miRNAs), a novel class of important gene-regulatory molecules, correlates with tumor growth, invasion, metastasis, and chemo resistance in gastric cancer (GC). Microarray analysis revealed that aberrant expressed microRNA-17 (miR-17) and DEDD were identified in GC. DEDD has been found to act as an endogenous suppressor of tumor growth and metastasis through epithelial-mesenchymal transition (EMT) process. However, the role of miRNA-17 (miR-17) has not been clearly evaluated in GC, thereby a series of in vitro experiments were performed in this study...
June 25, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29929000/telmisartan-generates-ros-dependent-upregulation-of-death-receptor-5-to-sensitize-trail-in-lung-cancer-via-inhibition-of-autophagy-flux
#8
Mohammad Rasheduzzaman, Ji-Hong Moon, Ju-Hee Lee, Uddin Md Nazim, Sang-Youel Park
Telmisartan broadly used for the treatment of hypertension that is also known for its anticancer properties. TRAIL has the potential to kill tumor cells with minimal toxicity in normal cells by binding to death receptors, DR4 and DR5. Unfortunately, these TRAIL-death receptors have failed as most human cancers are resistant to TRAIL-mediated apoptosis. In this study, we evaluated telmisartan as a novel TRAIL-DR5-targeting agent with the aim of rendering TRAIL-based cancer therapies more active. Herein, we demonstrated that telmisartan could sensitize TRAIL and enhance NSCLC tumor cell death...
June 18, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29906559/peroxiredoxin-5-prevents-iron-overload-induced-neuronal-death-by-inhibiting-mitochondrial-fragmentation-and-endoplasmic-reticulum-stress-in-mouse-hippocampal-ht-22-cells
#9
Dong Gil Lee, Min Kyoung Kam, Kyung Min Kim, Han Seop Kim, Oh-Shin Kwon, Hyun-Shik Lee, Dong-Seok Lee
Iron is an essential element for neuronal as well as cellular functions. However, Iron overload has been known to cause neuronal toxicity through mitochondrial fission, dysregulation of Ca2+ , endoplasmic reticulum (ER) stress, and reactive oxygen species (ROS) production. Nevertheless, the precise mechanisms of iron-induced oxidative stress and mitochondria- and ER-related iron toxicity in neuronal cells are not fully understood. In this study, we demonstrated that iron overload induces ROS production earlier in the ER than in the mitochondria, and peroxiredoxin 5 (Prx5), which is a kind of antioxidant induced by iron overload, prevents iron overload-induced mitochondrial fragmentation mediated by contact with ER and translocation of Drp1, by inhibiting ROS production and calcium/calcineurin pathway in HT-22 mouse hippocampal neuronal cells...
June 12, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29890225/mixomics-analysis-of-breast-cancer-long-non-coding-rna-linc01561-acts-as-cerna-involved-in-the-progression-of-breast-cancer
#10
Rui Jiang, Chunming Zhao, Binbin Gao, Jiawen Xu, Wei Song, Peng Shi
OBJECTIVE: This study aimed at finding the long non-coding RNA (lncRNA), miRNA and mRNA which played critical roles in breast cancer (BrCa) by using mixOmics R package. METHOD: The BrCa dataset were obtained from TCGA and then analyzed using "DESeq2" R package. Multivariate analyses were performed with the "mixOmics" R package and the first component of the stacked partial least-Squares discriminant analysis results were used for searching the interested lncRNA, miRNA and mRNA...
June 8, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29886152/mir-208a-enhances-cell-proliferation-and-invasion-of-gastric-cancer-by-targeting-sfrp1-and-negatively-regulating-meg3
#11
Hai-Bin Cui, Huai-E Ge, Yong-Sen Wang, Xi-Yong Bai
The present studies have identified that microRNAs function as regulators in different diseases including cancers. However, the expression patterns and underlying molecular mechanisms of miR-208a involved in gastric cancer (GC) remain little known. In the study, our results demonstrated that miR-208a expression was significantly increased in GC tissues compared with adjacent normal tissues by performing qRT-PCR. Higher miR-208a expression was association with lymph node metastasis and TNM stage in GC patients...
June 8, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29777777/mir-128-3p-directly-targets-vegfc-vegfr3-to-modulate-the-proliferation-of-lymphatic-endothelial-cells-through-ca-2-signaling
#12
Jie Zhou, Zhiyou He, Le Guo, Jizhang Zeng, Pengfei Liang, Licheng Ren, Minghua Zhang, Pihong Zhang, Xiaoyuan Huang
Lymphangiogenesis has been regarded as a physiological response to pathologic stimuli. The abnormal proliferation of lymphatic endothelial cell (LECs) and lymphangiogenesis is involved in the development of lymphatic disorders. Reportedly, VEGFC/VEGFR3 plays a key role in lymphangiogenesis; moreover, VEGFC/VEGFR3 exerts their cellular effects through activation of Ca2+ signaling in several cell types. Herein, we demonstrated that VEGFC significantly up-regulated LEC proliferation through VEGFR3; moreover, VEGFC/VEGFR3 induced Ca2+ signaling activation...
May 16, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29874548/spectroscopic-analysis-beyond-the-diffraction-limit
#13
Biqin Dong, Janel L Davis, Cheng Sun, Hao F Zhang
The recent surge in spectroscopic Single-Molecule Localization Microscopy (sSMLM) offers exciting new capabilities for combining single molecule imaging and spectroscopic analysis. Through the synergistic integration of super-resolution optical microscopy and single-molecule spectroscopy, sSMLM offers combined strengths from both fields. By capturing the full spectra of single molecule fluorescent emissions, sSMLM can distinguish minute spectroscopic variations from individual fluorescent molecules while preserving nanoscopic spatial localization precision...
August 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29864543/ubiquitination-friend-and-foe-in-cancer
#14
REVIEW
Mohammed A Mansour
Dynamic modulation and posttranslational modification of proteins are tightly controlled biological processes that occur in response to physiological cues. One such dynamic modulation is ubiquitination, which marks proteins for degradation via the proteasome, altering their localization, affecting their activity, and promoting or interfering with protein interactions. Hence, ubiquitination is crucial for a plethora of physiological processes, including cell survival, differentiation and innate and adaptive immunity...
August 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29857052/endothelial-replicative-senescence-delayed-by-the-inhibition-of-mtorc1-signaling-involves-microrna-107
#15
Eng-Soon Khor, Pooi-Fong Wong
Accumulation of senescent endothelial cells can contribute to endothelium dysfunction. Suppression of MTOR signaling has been shown to delay senescence but the mechanism that underpins this effect, particularly one that involves miRNAs, remains to be further defined. This study sought to identify miRNAs involved in MTORC1-mediated inhibition of replicative senescence in endothelial cells. Pre-senescent HUVECs were prolonged treated with low dose rapamycin (1 nM), an MTOR inhibitor. Rapamycin treatment down-regulated the phosphorylated MTOR, RPS6 and 4EBP1 expressions, which confirmed MTORC1 suppression...
August 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29852248/srrf-universal-live-cell-super-resolution-microscopy
#16
Siân Culley, Kalina L Tosheva, Pedro Matos Pereira, Ricardo Henriques
Super-resolution microscopy techniques break the diffraction limit of conventional optical microscopy to achieve resolutions approaching tens of nanometres. The major advantage of such techniques is that they provide resolutions close to those obtainable with electron microscopy while maintaining the benefits of light microscopy such as a wide palette of high specificity molecular labels, straightforward sample preparation and live-cell compatibility. Despite this, the application of super-resolution microscopy to dynamic, living samples has thus far been limited and often requires specialised, complex hardware...
August 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29807095/role-of-mirnas-in-cell-signaling-of-cancer-associated-fibroblasts
#17
REVIEW
A K Eichelmann, C Matuszcak, R Hummel, J Haier
The tumor microenvironment (TME) of cancer cells is regarded as a strong determinant for cancer development and acquisition of metastatic potential of cancer cells. Because of its influence on tumorigenesis, the TME increasingly gained attention in research within the last years. Activated fibroblasts, so-called cancer-associated fibroblasts (CAFs), which are the most prominent cell type in the stromal compartment, are responsible for the synthesis, deposition and remodeling of the extracellular matrix in tumor stroma thus creating a favorable microenvironment for cancer cells...
August 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29800727/identification-and-application-of-self-binding-zipper-like-sequences-in-sars-cov-spike-protein
#18
Si Min Zhang, Ying Liao, Tuan Ling Neo, Yanning Lu, Ding Xiang Liu, Anders Vahlne, James P Tam
Self-binding peptides containing zipper-like sequences, such as the Leu/Ile zipper sequence within the coiled coil regions of proteins and the cross-β spine steric zippers within the amyloid-like fibrils, could bind to the protein-of-origin through homophilic sequence-specific zipper motifs. These self-binding sequences represent opportunities for the development of biochemical tools and/or therapeutics. Here, we report on the identification of a putative self-binding β-zipper-forming peptide within the severe acute respiratory syndrome-associated coronavirus spike (S) protein and its application in viral detection...
August 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29800726/cellular-fluorescein-hyperfluorescence-is-dynamin-dependent-and-increased-by-tetronic-1107-treatment
#19
Tahmina F Khan, Bianca L Price, Philip B Morgan, Carole Maldonado-Codina, Curtis B Dobson
Sodium fluorescein ('fluorescein') staining of the ocular surface is frequently an indicator of compromised ocular health, and increases in the presence of certain contact lens multi-purpose solutions (MPS), a phenomenon known as solution induced corneal staining (SICS). The mechanism(s) underpinning fluorescein hyperfluorescence are uncertain, though may reflect increased cellular uptake of fluorescein by corneal epithelial cells. We have developed an in vitro model to study fluorescein uptake in both 'generic' mammalian cells (murine fibroblasts) and human corneal cells...
August 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29800725/mel-pep-an-analog-of-melittin-disrupts-cell-membranes-and-reverses-5-fluorouracil-resistance-in-human-hepatocellular-carcinoma-cells
#20
Mengyun Ke, Jian Dong, Yue Wang, Jia Zhang, Mei Zhang, Zheng Wu, Yi Lv, Rongqian Wu
Chemotherapy resistance represents a major obstacle in the treatment of patients with hepatocellular carcinoma (HCC). The purpose of this study was to investigate the anti-cancer effect of MEL-pep, a novel analog of the natural antibacterial peptide melittin (MEL), on human 5-fluorouracil-resistant HCC cells (BEL-7402/5-FU) and to clarify the molecular mechanisms involved in these effects. We found that MEL-pep inhibited the proliferation of BEL-7402/5-FU cells and reversed 5-FU resistance in vitro. MEL-pep directly bound to BEL-7402/5-FU cells and disrupted the cell membrane...
August 2018: International Journal of Biochemistry & Cell Biology
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