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International Journal of Biochemistry & Cell Biology

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https://www.readbyqxmd.com/read/28811250/deficiency-in-perilipin-5-reduces-mitochondrial-function-and-membrane-depolarization-in-mouse-hearts
#1
Linda Andersson, Christina Drevinge, Ismena Mardani, Knut T Dalen, Marcus Ståhlman, Martina Klevstig, Annika Lundqvist, Fred Haugen, Martin Adiels, Per Fogelstrand, Jorge Asin-Cayuela, Lillemor Mattsson Hultén, Max Levin, Ewa Ehrenborg, Yun K Lee, Alan R Kimmel, Jan Borén, Malin C Levin
Myocardial triglycerides stored in lipid droplets are important in regulating the intracellular delivery of fatty acids for energy generation in mitochondria, for membrane biosynthesis, and as agonists for intracellular signaling. Previously, we showed that deficiency in the lipid droplet protein perilipin 5 (Plin5) markedly reduces triglyceride storage in cardiomyocytes and increases the flux of fatty acids into phospholipids. Here, we investigated whether Plin5 deficiency in cardiomyocytes alters mitochondrial function...
August 12, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28807601/ubx-domain-containing-proteins-are-involved-in-lipid-homeostasis-and-stress-responses-in-pichia-pastoris
#2
Meng Zhang, Qilin Yu, Zhe Liu, Chen Liang, Biao Zhang, Mingchun Li
Ubiquitin regulatory X (UBX) domain-containing proteins constitute a family of proteins and are substrate adaptors of AAA ATPase Cdc48. UBX proteins can bind to the N-terminal region of Cdc48 to perform endoplasmic reticulum associated protein degradation (ERAD). In this study, we identified two UBX domain-containing proteins, Ubx1 and Ubx2, in Pichia pastoris and found that the two proteins could recover the growth defect of Saccharomyces cerevisiae in ubx2Δ. Our results revealed that Ubx1 and Ubx2 play critical roles in synthesis of unsaturated fatty acids by affecting Spt23...
August 11, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28807600/methylglyoxal-synthase-regulates-cell-elongation-via-alterations-of-cellular-methylglyoxal-and-spermidine-content-in-bacillus-subtilis
#3
Sang-Min Shin, Sung-Hyun Song, Jin-Woo Lee, Min-Kyu Kwak, Sa-Ouk Kang
Methylglyoxal regulates cell division and differentiation through its interaction with polyamines. Loss of their biosynthesizing enzyme causes physiological impairment and cell elongation in eukaryotes. However, the reciprocal effects of methylglyoxal and polyamine production and its regulatory metabolic switches on morphological changes in prokaryotes have not been addressed. Here, Bacillus subtilis methylglyoxal synthase (mgsA) and polyamine biosynthesizing genes encoding arginine decarboxylase (SpeA), agmatinase (SpeB), and spermidine synthase (SpeE), were disrupted or overexpressed...
August 11, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28802562/current-research-development-of-single-cell-genome-in-urological-tumor
#4
Ziyi Cao, Song Wu
The technique of whole genome amplification is advancing rapidly and generating attention on detecting genomic lesions in individual cancer cells. Also, single-cell genome could label the uniqueness of each cell, its individual mutations and structural variations especially in cancer studies. In this Review, we provide the insight into the current state of single-cell genome in urological tumor mainly including kidney cancer, bladder cancer and prostate cancer. We put more forward on the new progress of the technique used by single-cell genomes and different results of the genes transform on random tumor tissue from single cell isolated on account of tumor heterogeneity...
August 9, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28802561/recombinant-human-elafin-promotes-alveologenesis-in-newborn-mice-exposed-to-chronic-hyperoxia
#5
Wenli Han, Xiaomei Li, Han Zhang, Benli Yu, Chunbao Guo, Chun Deng
BACKGROUND/AIMS: Elastase inhibitors reverse elastin degradation and abnormal alveologenesis and attenuate the lung structural abnormalities induced by mechanical ventilation with O2-rich gas. The potential of these molecules to improve endothelial function and to ameliorate severe bronchopulmonary dysplasia (BPD) during lung development is not yet understood. We sought to determine whether the intratracheal treatment of newborn mice with the elastase inhibitor elafin would prevent hyperoxia-induced lung elastin degradation and the cascade of events that cause abnormal alveologenesis...
August 9, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28789920/the-%C3%AF-carboxyl-group-of-7-ketocholesteryl-9-carboxynonanoate-mediates-the-binding-of-oxldl-to-cd36-receptor-and-enhances-caveolin-1-expression-in-macrophages
#6
Jingda Li, Chengjie Yu, Renjun Wang, Jianrong Xu, Yan Chi, Jianzhong Qin, Qingping Liu
CD36 signal transduction modulates the uptake of oxidized low-density lipoprotein (oxLDL) and foam cell formation. We previously observed that 7-ketocholesteryl-9-carboxynonanoate (oxLig-1), the lipid moiety of oxLDL, activates the CD36-Src-JNK/ERK1/2 signalling pathway. In this study, we assessed the role of the ω-carboxyl group in the binding of oxLig-1 to CD36 and investigated whether the binding of the ω-carboxyl group to CD36 triggers CD36-mediated signalling, thereby resulting in the upregulation of caveolin-1 expression...
August 5, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28782601/metabolomic-profile-in-hyperthyroid-patients-before-and-after-antithyroid-drug-treatment-correlation-with-thyroid-hormone-and-tsh-concentration
#7
Cristina Piras, Nicolò Arisci, Simone Poddighe, Sonia Liggi, Stefano Mariotti, Luigi Atzori
Hyperthyroidism (HT) is characterized by an intense metabolic impact which affects the lipid, carbohydrate and amino acids metabolism, with increased resting energy expenditure and thermogenesis. Metabolomics is a new comprehensive technique that allows to capture an instant metabolic picture of an organism, reflecting peculiar molecular and pathophysiological states. The aim of the present prospective study was to identify a distinct metabolomic profile in HT patients using (1)H NMR spectroscopy before and after antithyroid drug treatment...
August 4, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28782600/mir-18a-induces-myotubes-atrophy-by-down-regulating-igfi
#8
Chuncheng Liu, Meng Wang, Min Chen, Kuo Zhang, Lijie Gu, Qiuyan Li, Zhengquan Yu, Ning Li, Qingyong Meng
Muscle atrophy occurs when there is a net loss of muscle mass, leading to a change in the balance between protein synthesis and protein degradation. Igf1 is important for protein synthesis in muscle cells and can induce local skeletal muscle hypertrophy and attenuate age-related skeletal muscle atrophy via the PI3K/Akt pathway in mice, consequently restoring and improving muscle mass and strength. In this study, we show that miR-18a expression is down-regulated during C2C12 myoblast differentiation and mouse tibialis anterior muscle postnatal development...
August 4, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28778674/calreticulin-regulates-tgf-%C3%AE-1-induced-epithelial-mesenchymal-transition-through-modulating-smad-signaling-and-calcium-signaling
#9
Yanjiao Wu, Xiaoli Xu, Lunkun Ma, Qian Yi, Weichao Sun, Liling Tang
As a Ca(2+) binding protein, calreticulin (CRT) has many functions and plays an important role in a variety of tumors. The role of CRT in TGF-β1-induced EMT is unknown. In this study, we demonstrated in vitro that TGF-β1-induced EMT elevated the expression of CRT in A549 lung cancer cells. Subsequently, we confirmed that overexpression CRT had no capacity to induce A549 cells EMT alone, but successfully enhanced TGF-β1-induced-EMT. Furthermore, knockdown of CRT in A549 cells significantly suppressed changes of EMT marks expression induced by TGF-β1...
August 1, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28760625/methylglyoxal-upregulates-dictyostelium-discoideum-slug-migration-by-triggering-glutathione-reductase-and-methylglyoxal-reductase-activity
#10
Hyang-Mi Lee, Ji-Hui Seo, Min-Kyu Kwak, Sa-Ouk Kang
Glutathione (GSH)-deprived Dictyostelium discoideum accumulates methylglyoxal (MG) and reactive oxygen species (ROS) during vegetative growth. However, the reciprocal effects of the production and regulation of these metabolites on differentiation and cell motility are unclear. Based on the inhibitory effects of γ-glutamylcysteine synthetase (gcsA) disruption and GSH reductase (gsr) overexpression on aggregation and culmination, respectively, we overexpressed GSH-related genes encoding superoxide dismutase (Sod2), catalase (CatA), and Gcs, in D...
July 29, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28757458/a-computational-integrative-approach-based-on-alternative-splicing-analysis-to-compare-immortalized-and-primary-cancer-cells
#11
Kumar Parijat Tripathi, Ilaria Granata, Mario Rosario Guarracino
Immortalized cell lines are widely used to study the effectiveness and toxicity of anti cancer drugs as well as to assess the phenotypic characteristics of cancer cells, such as proliferation and migration ability. Unfortunately, cell lines often show extremely different properties than tumor tissues. Also the primary cells, that are deprived of the in vivo environment, might adapt to artificial conditions, and differ from the tissue they should represent. Despite these considerations, cell lines are still one of the most used cancer models due to their availability and capability to expand without limitation, but the clinical relevance of their use is still a big issue in cancer research...
July 27, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28754317/natural-antisense-transcript-tpm1-as-regulates-the-alternative-splicing-of-tropomyosin-i-through-an-interaction-with-rna-binding-motif-protein-4
#12
Guo-Wei Huang, Ying-Li Zhang, Lian-Di Liao, En-Min Li, Li-Yan Xu
LncRNAs play a vital role in alternative splicing of target genes. However, the mechanisms underlying lncRNAs involvement in splicing are poorly understood. In the present study, we identified a previously uncharacterized lncRNA, which is denoted as TPM1-AS, is reverse-transcribed from the fourth intronic region of the tropomyosin I (TPM1). In situ hybridization and RNA immunoprecipitation assays demonstrated that TPM1-AS was located in the nucleus and interacted with RNA-binding motif protein 4 (RBM4) in human esophageal cancer cells...
July 25, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28754316/patterns-and-functional-implications-of-platelets-upon-tumor-education
#13
REVIEW
Qun Zhang, Hongda Liu, Qingqing Zhu, Ping Zhan, Suhua Zhu, Jianya Zhang, Tangfeng Lv, Yong Song
While platelets are traditionally recognized to play a predominant role in hemostasis and thrombosis, increasing evidence verifies its involvement in malignancies. As a component of the tumor microenvironment, platelets influence carcinogenesis, tumor metastasis and chemotherapy efficiency. Platelets status is thus predictable as a hematological biomarker of cancer prognosis and a hot target for therapeutic intervention. On the other hand, the role of circulating tumor cells (CTCs) as an inducer of platelet activation and aggregation has been well acknowledged...
July 25, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28751193/microrna-33a-and-let-7e-inhibit-human-colorectal-cancer-progression-by-targeting-st8sia1
#14
Yujia Shan, Yuejian Liu, Lifen Zhao, Bing Liu, Yang Li, Li Jia
Colorectal cancer (CRC) is one of the leading causes of cancer mortality worldwide. Aberrant sialylation is crucially involved in the progression of various types of cancer. MicroRNAs (miRNAs) have been broadly studied in cancer. MicroRNA-33a (miR-33a) and Has-let-7e (let-7e) are non-coding RNA that can reduce cell motility and viability in cancer. In this study, miR-33a and let-7e levels were confirmed to be significantly down-regulated in CRC samples (n=32) and drug resistant cell line (HCT-8/5-FU) compared with those in the matched adjacent tissues and drug sensitivity cell line (HCT-8)...
July 24, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28743675/role-of-nmd-and-nas-in-the-mrna-pattern-of-two-new-%C3%AE-thalassemia-mutants
#15
Giovanna Cardiero, Clelia Scarano, Gennaro Musollino, Francesca Di Noce, Romeo Prezioso, Sabrina Dembech, Gaetana La Porta, Maria Grazia Bisconte, Rosario Colella, Giuseppina Lacerra
The α-thalassemia is a common disease due in prevalence to deletional mutants. We have identified two new α-thalassemia pointform mutants: α1 cod22 GGC > GGT Gly > Gly creating a 5' splicing sequence and: α1 cod23 GAG >TAG Glu > stop. We perform a qualitative and semi-quantitative analysis of the mRNA molecules, from blood of the carriers, to define the molecular mechanisms giving rise to thalassemia phenotype. In vitro analysis using minigenes and cycloheximide was performed to evaluate if the mutants are substrate of nonsense-mediated mRNA decay...
July 22, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28743674/the-role-of-alternative-splicing-coupled-to-nonsense-mediated-mrna-decay-in-human-disease
#16
Paulo J da Costa, Juliane Menezes, Luísa Romão
Alternative pre-mRNA splicing (AS) affects gene expression as it generates proteome diversity. Nonsense-mediated mRNA decay (NMD) is a surveillance pathway that recognizes and selectively degrades mRNAs carrying premature translation-termination codons (PTCs), preventing the production of truncated proteins that could result in disease. Several studies have also implicated NMD in the regulation of steady-state levels of physiological mRNAs. In addition, it is known that several regulated AS events do not lead to generation of protein products, as they lead to transcripts that carry PTCs and thus, they are committed to NMD...
July 22, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28739533/effect-of-bisphenol-a-bpa-on-insulin-signal-transduction-and-glut4-translocation-in-gastrocnemius-muscle-of-adult-male-albino-rat
#17
Vigneswari Mullainadhan, Mangala Priya Viswanathan, Balasubramanian Karundevi
Environmental estrogens bind to estrogen receptors, mimic estrogenic actions, and have adverse effects on human health like Bisphenol - A (BPA) which is used as a monomer in the production of polycarbonate plastics (PC) and epoxy resins which are used in variety of canned foods. Skeletal muscle plays an essential role in maintaining systemic glucose metabolism. In the present study, we investigated the possible effects of BPA on insulin signalling molecules and GLUT4 translocation in the gastrocnemius muscle of adult male rat...
July 21, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28736244/the-use-of-stable-isotopes-in-the-study-of-human-pathophysiology
#18
Evelina Charidemou, Tom Ashmore, Julian L Griffin
The growing prevalence of metabolic diseases including fatty liver disease and Type 2 diabetes has increased the emphasis on understanding metabolism at the mechanistic level and how it is perturbed in disease. Metabolomics is a continually expanding field that seeks to measure metabolites in biological systems during a physiological stimulus or a genetic alteration. Typically, metabolomics studies provide total pool sizes of metabolites rather than dynamic flux measurements. More recently there has been a resurgence in approaches that use stable isotopes (e...
July 20, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28736243/from-insulin-synthesis-to-secretion-alternative-splicing-of-type-2-ryanodine-receptor-gene-is-essential-for-insulin-secretion-in-pancreatic-%C3%AE-cells
#19
Hiroshi Okamoto, Shin Takasawa, Yasuhiko Yamamoto
Increases in the intracellular Ca(2+) concentration in pancreatic islets, resulting from the its mobilization from the intracellular source through the ryanodine receptor, are essential for insulin secretion by glucose. Cyclic ADP-ribose, a potent Ca(2+) mobilizing second messenger synthesized from NAD(+) by CD38, regulates the opening of ryanodine receptor. A novel ryanodine receptor mRNA (the islet-type ryanodine receptor) was found to be generated from the type 2 ryanodine receptor gene by the alternative splicing of exons 4 and 75...
July 20, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28734834/dynein-binds-and-stimulates-axonal-motility-of-the-endosome-adaptor-and-neep21-family-member-calcyon
#20
Liang Shi, Nagendran Muthusamy, Deanna Smith, Clare Bergson
The neuron-enriched, endosomal protein Calcyon (Caly) regulates endocytosis and vesicle sorting, and is important for synaptic plasticity and brain development. In the current investigation of Caly interacting proteins in brain, the microtubule retrograde motor subunit, cytoplasmic dynein 1 heavy chain (DYNC1H), and microtubule structural proteins, α and β tubulin, were identified as Caly associated proteins by MALDI-ToF/ToF. Direct interaction of the Caly-C terminus with dynein and tubulin was further confirmed in in vitro studies...
July 19, 2017: International Journal of Biochemistry & Cell Biology
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