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International Journal of Biochemistry & Cell Biology

Seok Soon Park, Eunjin Jwa, Seol Hwa Shin, Eun Jin Ju, Intae Park, Jhang Ho Pak, Jung Jin Hwang, Dong-Hyung Cho, B Moon Kim, Sung-Bae Kim, Jung Shin Lee, Si Yeol Song, Seong-Yun Jeong, Eun Kyung Choi
Tumor necrosis factor-related apoptosis-induced ligand (TRAIL) induces apoptosis selectively in cancer cells without affecting the majority of normal human cells. However, hepatocellular carcinoma (HCC) cells often display resistance to TRAIL-induced apoptosis. Ibulocydine (IB) is an isobutyrate ester pro-drug of a novel synthetic Cdk inhibitor that targets Cdk7 and Cdk9. In this study, we show that treatment with subtoxic doses of IB in combination with TRAIL displays potent cytotoxicity in TRAIL-resistant human HCC cells...
December 3, 2016: International Journal of Biochemistry & Cell Biology
Shaoni Dasgupta, Subhajit Dasgupta
Generation of myelin antigen-specific T cells is a major event in neuroimmune responses that causes demyelination. The antigen-priming of T cells and its location is important in chronic and acute inflammation. In autoimmune multiple sclerosis, the effector T cells are considered to generate in periphery. However, the reasons for chronic relapsing-remitting events are obscure. Considering mechanisms, a feasible aim of research is to investigate the role of antigen-primed T cells in lupus cerebritis. Last thirty years of investigations emphasize the relevance of microglia and infiltrated dendritic cells/macrophages as antigen presenting cells in the central nervous system...
November 27, 2016: International Journal of Biochemistry & Cell Biology
Suvasmita Rath, Lopamudra Das, Shrikant Babanrao Kokate, Nilabh Ghosh, Pragyesh Dixit, Niranjan Rout, Shivaram P Singh, Subhasis Chattopadhyay, Hassan Ashktorab, Duane T Smoot, Mahadeva M Swamy, Tapas K Kundu, Sheila E Crowe, Asima Bhattacharyya
Hypoxia enhances immortality and metastatic properties of solid tumors. Deregulation of histone acetylation has been associated with several metastatic cancers but its effect on hypoxic responses of cancer cells is not known. This study aimed at understanding the effectiveness of the hydrazinocurcumin, CTK7A, an inhibitor of p300 lysine/histone acetyltransferase (KAT/HAT) activity, in inducing apoptosis of gastric cancer cells (GCCs) exposed to cobalt chloride (CoCl2), a hypoxia-mimetic chemical, or 1% O2. Here, we show that CTK7A-induced hydrogen peroxide (H2O2) generation in CoCl2-exposed and invasive gastric cancer cells (GCCs) leads to p38 MAPK-mediated Noxa expression and thereafter, mitochondrial apoptotic events...
November 23, 2016: International Journal of Biochemistry & Cell Biology
Xuepeng Chen, Songying Li, Zhaobin Zeng, Zexu Gu, Yanfang Yu, Feifei Zheng, Yi Zhou, Huiming Wang
Dental follicle stem cells (DFSCs) have been considered as promising candidate cells for periodontal tissue regeneration. Understanding the signalling pathways underlying the apoptosis of DFSCs will facilitate its biomedical application. Here we showed that Notch1 signalling could inhibit DFSCs apoptosis because the constitutive overexpression of the intracellular domain of Notch1 (ICN1) promoted proliferation and suppressed apoptosis by inhibiting cytoplasmic mitochondrial membrane depolarization, cytochrome c release and activation of caspase-9 and caspase-3...
November 22, 2016: International Journal of Biochemistry & Cell Biology
Irmgard Irminger-Finger, Joanna Kargul, Geoffrey J Laurent
No abstract text is available yet for this article.
November 19, 2016: International Journal of Biochemistry & Cell Biology
Anita Boratkó, Margit Péter, Csilla Csortos
Merlin (moesin-ezrin-radixin like protein), the product of neurofibromatosis type 2 gene, was primarily recognized as a tumor suppressor, but it also functions as a membrane-cytoskeletal linker and regulator of multiple signaling pathways. The activity and localization of merlin is regulated by head to tail folding that is controlled by phosphorylation of the Ser518 side chain. Merlin localizes in the nucleus when the Ser518 side chain is not phosphorylated, while the phosphorylated form is present in the cytoplasm and the plasma membrane...
November 18, 2016: International Journal of Biochemistry & Cell Biology
Athanasios Pseftogas, Christos Gonidas, George Mosialos
Several studies have implicated the downregulation of the tumor suppressor Cyld expression in breast cancer development. However, the mechanisms that regulate Cyld expression in mammary epithelial cells are largely unknown. In order to investigate them, a bioinformatic analysis of the promoter region of Cyld was performed and identified putative nuclear hormone receptor response elements that included peroxisome proliferator-activated receptor gamma (PPAR-γ)-responsive elements. In the present study, we showed that upon activation of the nuclear hormone receptor PPAR-γ by the agonist troglitazone (TZD), there was a significant increase in Cyld mRNA in human mammary epithelial cell lines...
November 16, 2016: International Journal of Biochemistry & Cell Biology
Miao Yu, Si-Qi Yi, Yan-Ru Wu, Hua-Ling Sun, Fang-Fang Song, Jia-Wei Wang
Endochondral ossification is an essential skeletal development process which is strongly linked to chondrocyte differentiation. DNA damage-inducible transcript 3 (Ddit3), a member of the CCAAT/enhancer-binding protein family of transcription factors, is highly expressed in the cartilage plate. However, the role of DNA damage-inducible transcript 3 in chondrocyte differentiation remains to be investigated. Immunofluorescent staining was used to detect Ddit3 expression in the mouse growth plate and in the mouse chondroprogenitor cell line ATDC5...
November 11, 2016: International Journal of Biochemistry & Cell Biology
Irina V Sorokina, Tatiana V Denisenko, Gabriela Imreh, Vladimir Gogvadze, Boris Zhivotovsky
Mitotic catastrophe (MC) is a sequence of events resulting from premature or inappropriate entry of cells into mitosis that can be caused by chemical or physical stresses. There are several observations permitting to define MC as an oncosuppressive mechanism. MC can end up in apoptosis, necrosis or senescence. Here we show that the anticancer drug doxorubicin triggers DNA damage and MC independently of ROS production. In contrast, doxorubicin-induced apoptosis was found to be ROS-dependent. Antioxidants NAC or Trolox suppressed apoptosis, but facilitated MC development...
November 11, 2016: International Journal of Biochemistry & Cell Biology
Manisha Banerjee, Moumita Datta, Nitai P Bhattacharyya
Increased poly glutamine (polyQ) stretch at N-terminal of Huntingtin (HTT) causes Huntington's disease. HTT interacts with large number of proteins, although the preference for such interactions with wild type or mutated HTT protein remains largely unknown. HYPK, an intrinsically unstructured protein chaperone and interactor of mutant HTT was found to interact with myeloid leukemia factor 1 (MLF1) and 2 (MLF2). To identify the role of these two proteins in mutant HTT mediated aggregate formation and toxicity in a cell model, both the proteins were found to preferentially interact with the mutated N-terminal HTT...
November 10, 2016: International Journal of Biochemistry & Cell Biology
Hamid Maadi, Abdolvahab Moshtaghian, Masoumeh Fakhr Taha, Seyed Javad Mowla, Asiye Kazeroonian, Nikolas K Haass, Arash Javeri
The miR-302 family is one of the main groups of microRNAs, which are highly expressed in embryonic stem cells (ESCs). Previous reports have indicated that miR-302 can reduce the proliferation rate of some cancer cells while compromising on their oncogenic potential at the same time without having the same effect on normal somatic cells. In this study we aimed to further investigate the role of the miR-302 cluster in multiple cancer signaling pathways using A-375 melanoma and HT-29 colorectal cancer cells. Our results indicate that the miR-302 cluster has the potential to modulate oncogenic properties of cancer cells through inhibition of proliferation, angiogenesis and invasion, and through reversal of the epithelial-to-mesenchymal transition (EMT) in these cells...
November 10, 2016: International Journal of Biochemistry & Cell Biology
Paul G Thomes, Elizabeth Brandon-Warner, Ting Li, Terrence M Donohue, Laura W Schrum
We demonstrated that ligand-activated nuclear receptor Rev-erbα mitigates CCl4-induced liver fibrosis. Rev-erbα is also a novel regulator of autophagy, a crucial eukaryotic catabolic system in which lysosomes degrade substrates for energy generation. In hepatic stellate cells (HSC) autophagy is reportedly required for this purpose to activate HSCs during fibrogenesis. Here, we examined whether pharmacological activation of Rev-erb with its synthetic ligand SR9009 or treatment with the pro-fibrotic cytokine, TGF-β, each differentially modulate autophagy to regulate the HSC phenotype...
November 10, 2016: International Journal of Biochemistry & Cell Biology
Hugh Ip, Tariq Sethi
CD98 is implicated in a large number of cancers. CD98 regulates amino acid transport and integrin signaling, which are key drivers in tumor progression. The light chain in the CD98 heterodimer functions as an amino acid exchanger. Alongside the light chain, the heavy chain forms a heterocomplex at the plasma membrane to mediate signaling pathways. In this article, we review various approaches to blocking CD98 activity, which may lead to novel opportunities in cancer therapeutics.
November 10, 2016: International Journal of Biochemistry & Cell Biology
Nadia Nathan, Jessica Taytard, Philippe Duquesnoy, Guillaume Thouvenin, Harriet Corvol, Serge Amselem, Annick Clement
The respiratory tract is continually exposed to various insults that are a permanent threat to the maintenance of lung homeostasis. Repair of the parenchyma structure, particularly of the alveolar epithelium, requires complex cellular strategies. Among the molecular components that play an important role in these processes are the surfactant proteins (SPs), particularly SP-A. The present review examines current evidence regarding the role of SP-A in lung host defence mechanisms through its implication in innate/adaptive immunity of the lung and epithelium integrity and repair...
November 9, 2016: International Journal of Biochemistry & Cell Biology
Giuseppe Valacchi, Carlo Cervellati, Pablo Evelson, Irfan Rhaman
No abstract text is available yet for this article.
November 3, 2016: International Journal of Biochemistry & Cell Biology
Lan Deng, Xiuju Wang, Ling Jiang, Jilong Yang, Xuan Zhou, Zhigang Lu, Haiyan Hu
Immune thrombocytopenia (ITP) is an acquired autoimmune disorder characterized by low platelet count and bleeding, and is usually triggered by viral infections. We previously reported that 14 viral microRNAs of megakaryocytes cultured with serum from patients with ITP, including ebv-miR-BART6, are up-regulated. Previous research has reported that ebv-miR-BART6 down-regulated the expression of miR-185-5p. We therefore predicted that the ABCG4 gene, which is highly expressed in megakaryocyte progenitor cells, is a direct target of miR-185-5p...
November 2, 2016: International Journal of Biochemistry & Cell Biology
Gi-Jun Sung, Hyo-Kyoung Choi, Sungmin Kwak, Ji-Hye Song, Hyeonseok Ko, Ho-Geun Yoon, Hee-Bum Kang, Kyung-Chul Choi
Cancer cells are characterized by altered metabolic processes. Recent evidence of metabolic alterations has indicated that the fatty acid oxidation (FAO) pathway is used as a carbon source for anabolic processes in some tumors, thus making this pathway a potential target for therapy. The carnitine palmitoyltransferase (CPT; EC enzyme transfers long-chain fatty acids from the cytosol to the mitochondrial matrix for β-oxidation. Because carnitine palmitoyl transferase 1a (CPT1a) is the rate-limiting enzyme for FAO, the authors evaluated the effects of CPT1A knock-down in BRAF V600E melanoma cell lines...
October 25, 2016: International Journal of Biochemistry & Cell Biology
Paula E Tenconi, Norma M Giusto, Gabriela A Salvador, Melina V Mateos
Inflammation is a key factor in the pathogenesis of several retinal diseases. In view of the essential role of the retinal pigment epithelium in visual function, elucidating the molecular mechanisms elicited by inflammation in this tissue could provide new insights for the treatment of retinal diseases. The aim of the present work was to study protein kinase C signaling and its modulation by phospholipases D in ARPE-19 cells exposed to lipopolysaccharide. This bacterial endotoxin induced protein kinase C-α/βII phosphorylation and protein kinase-ε translocation to the plasma membrane in ARPE-19 cells...
October 25, 2016: International Journal of Biochemistry & Cell Biology
Erica T Goddard, Ryan C Hill, Alexander Barrett, Courtney Betts, Qiuchen Guo, Ori Maller, Virginia F Borges, Kirk C Hansen, Pepper Schedin
Normal epithelium exists within a dynamic extracellular matrix (ECM) that is tuned to regulate tissue specific epithelial cell function. As such, ECM contributes to tissue homeostasis, differentiation, and disease, including cancer. Though it is now recognized that the functional unit of normal and transformed epithelium is the epithelial cell and its adjacent ECM, we lack a basic understanding of tissue-specific ECM composition and abundance, as well as how physiologic changes in ECM impact cancer risk and outcomes...
October 24, 2016: International Journal of Biochemistry & Cell Biology
Michela Novelli, Marta Menegazzi, Pascale Beffy, Svetlana Porozov, Alex Gregorelli, Daniela Giacopelli, Vincenzo De Tata, Pellegrino Masiello
The extract of the herbaceous plant St. John's wort (SJW) and its phloroglucinol component hyperforin (HPF) were previously shown to inhibit cytokine-induced STAT-1 and NF-κB activation and prevent damage in pancreatic β cells. To further clarify the mechanisms underlying their protective effects, we evaluated the phosphorylation state of various factors of cytokine signaling pathways and the expression of target genes involved in β-cell function, inflammatory response and apoptosis induction. In the INS-1E β-cell line, exposed to a cytokine mixture with/without SJW extract (2-5μg/ml) or HPF (1-5μM), protein phosphorylation was assessed by western blotting and expression of target genes by real-time quantitative PCR...
October 22, 2016: International Journal of Biochemistry & Cell Biology
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