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Histochemistry and Cell Biology

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https://www.readbyqxmd.com/read/28936549/in-focus-in-hcb
#1
EDITORIAL
Douglas J Taatjes, Jürgen Roth
No abstract text is available yet for this article.
September 21, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28875375/germ-cell-line-during-the-seasonal-sexual-rest-of-clams-finding-niches-of-cells-for-gonad-renewal
#2
Liliana Milani, Andrea Pecci, Fabrizio Ghiselli, Marco Passamonti, Maurizio Lazzari, Valeria Franceschini, Maria Gabriella Maurizii
Reconstitution and renewal of tissues are key topics in developmental biology. In this brief work, we analyzed the wintry spent phase of the reproductive cycle in the Manila clam Ruditapes philippinarum (Bivalvia, Veneridae) in order to study the gonad rebuilding that in this species occurs at the beginning of the warmer months. We labeled VASA homolog protein-a germ cell marker-and compared the histological observations of the spent phase with those of the previously analyzed gametogenic phase. In R. philippinarum, during the reproductive season, most of the body mass is represented by sack-like structures (acini) full of developing gametes...
September 5, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28828681/role-of-epithelial-mesenchymal-transition-involved-molecules-in-the-progression-of-cutaneous-melanoma
#3
Daniela Murtas, Cristina Maxia, Andrea Diana, Luca Pilloni, Claudia Corda, Luigi Minerba, Sara Tomei, Franca Piras, Caterina Ferreli, Maria Teresa Perra
Epithelial-mesenchymal transition (EMT) has been suggested to have a driving role in the acquisition of a metastatic potential by melanoma cells. Important hallmarks of EMT include both E-cadherin downregulation and increased expression of N-cadherin. This switch in distinct classes of adhesion molecules leads melanoma cells to lose contact with adjacent keratinocytes and interact instead with stromal fibroblasts and endothelial cells, thus promoting dermal and vascular melanoma invasion. Consequently, tumor cells migrate to distant host tissues and establish metastases...
August 21, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28825130/recapitulating-in-vivo-like-plasticity-of-glioma-cell-invasion-along-blood-vessels-and-in-astrocyte-rich-stroma
#4
Pavlo Gritsenko, William Leenders, Peter Friedl
Diffuse invasion of glioma cells into the brain parenchyma leads to nonresectable brain tumors and poor prognosis of glioma disease. In vivo, glioma cells can adopt a range of invasion strategies and routes, by moving as single cells, collective strands and multicellular networks along perivascular, perineuronal and interstitial guidance cues. Current in vitro assays to probe glioma cell invasion, however, are limited in recapitulating the modes and adaptability of glioma invasion observed in brain parenchyma, including collective behaviours...
August 19, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28821957/co-culture-of-chondrons-and-mesenchymal-stromal-cells-reduces-the-loss-of-collagen-vi-and-improves-extracellular-matrix-production
#5
H A Owida, T De Las Heras Ruiz, A Dhillon, Y Yang, N J Kuiper
Adult articular chondrocytes are surrounded by a pericellular matrix (PCM) to form a chondron. The PCM is rich in hyaluronan, proteoglycans, and collagen II, and it is the exclusive location of collagen VI in articular cartilage. Collagen VI anchors the chondrocyte to the PCM. It has been suggested that co-culture of chondrons with mesenchymal stromal cells (MSCs) might enhance extracellular matrix (ECM) production. This co-culture study investigates whether MSCs help to preserve the PCM and increase ECM production...
August 19, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28821965/increased-endocytosis-of-magnetic-nanoparticles-into-cancerous-urothelial-cells-versus-normal-urothelial-cells
#6
Jasna Lojk, Vladimir Boštjan Bregar, Klemen Strojan, Samo Hudoklin, Peter Veranič, Mojca Pavlin, Mateja Erdani Kreft
The blood-urine barrier is the tightest and most impermeable barrier in the body and as such represents a problem for intravesical drug delivery applications. Differentiation-dependent low endocytotic rate of urothelial cells has already been noted; however, the differences in endocytosis of normal and cancer urothelial cells have not been exploited yet. Here we analysed the endocytosis of rhodamine B isothiocyanate-labelled polyacrylic acid-coated cobalt ferrite nanoparticles (NPs) in biomimetic urothelial in vitro models, i...
August 18, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28815335/in-focus-in-hcb
#7
EDITORIAL
Douglas J Taatjes, Jürgen Roth
No abstract text is available yet for this article.
August 17, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28755317/early-inflammatory-profiling-of-schwannoma-cells-induced-by-lipopolysaccharide
#8
Marcela Kohoutková, Andrea Korimová, Václav Brázda, Jiří Kohoutek, Petr Dubový
Inflammatory profiling of Schwann cells manifested as an upregulation of cytokines is present after traumatic or disease injury of the peripheral nerves. Inflammatory activation of Schwann cells via Toll-like receptors (TLRs) can be triggered by exogenous pathological molecules or endogenous ligands produced during Wallerian degeneration. We investigated the early period of inflammatory reactions by following the levels of TLR4, NFκB, IL-1β, pSTAT3, and IL-6 proteins after LPS treatment of RT4 schwannoma cells under in vitro conditions...
July 28, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28755316/deficiency-of-a-membrane-skeletal-protein-4-1g-results-in-myelin-abnormalities-in-the-peripheral-nervous-system
#9
Yurika Saitoh, Nobuhiko Ohno, Junji Yamauchi, Takeharu Sakamoto, Nobuo Terada
We previously demonstrated that a membrane skeletal molecular complex, 4.1G-membrane palmitoylated protein 6 (MPP6)-cell adhesion molecule 4, is incorporated in Schwann cells in the peripheral nervous system (PNS). In this study, we evaluated motor activity and myelin ultrastructures in 4.1G-deficient (-/-) mice. When suspended by the tail, aged 4.1G(-/-) mice displayed spastic leg extension, especially after overwork. Motor-conduction velocity in 4.1G(-/-) mice was slower than that in wild-type mice. Using electron microscopy, 4...
July 28, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28748255/cellular-and-subcellular-localization-of-adp-ribosylation-factor-6-in-mouse-peripheral-tissues
#10
Osamu Katsumata, Momoko Mori, Yusuke Sawane, Tomoko Niimura, Akiko Ito, Hirotsugu Okamoto, Masahiro Fukaya, Hiroyuki Sakagami
ADP-ribosylation factor 6 (Arf6) is a small GTPase that regulates endosomal trafficking and actin cytoskeleton remodeling. In the present study, we comprehensively examined the cellular and subcellular localization of Arf6 in adult mouse peripheral tissues by immunofluorescence and immunoelectron microscopy using the heat-induced antigen retrieval method with Tris-EDTA buffer (pH 9.0). Marked immunolabeling of Arf6 was observed particularly in epithelial cells of several tissues including the esophagus, stomach, small and large intestines, trachea, kidney, epididymis, oviduct, and uterus...
July 26, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28721450/smad3-and-bmal1-regulate-p21-and-s100a4-expression-in-myocardial-stromal-fibroblasts-via-tnf-%C3%AE
#11
Fuyuki Sato, Akira Kohsaka, Kana Takahashi, Saki Otao, Yusuke Kitada, Yoshiyuki Iwasaki, Yasuteru Muragaki
Bmal1, a clock gene, is associated with depression, hypertrophy, metabolic syndrome and diabetes. Smad3, which is involved in the TGF-β signaling pathway, plays an important role in the regulation of tumor progression, fibrosis, obesity and diabetes. Our previous report showed that Smad3 has circadian expression in mouse livers. In the current study, we focused on the heart, especially on the myocardial stromal fibroblasts because the roles of Bmal1 and Smad3 in this tissue are poorly understood. Bmal1 and Smad3 have circadian expression in mouse hearts, and their circadian expression patterns were similar...
July 18, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28714056/quality-control-in-diagnostic-immunohistochemistry-integrated-on-slide-positive-controls
#12
A Bragoni, A Gambella, S Pigozzi, M Grigolini, R Fiocca, L Mastracci, F Grillo
Standardization in immunohistochemistry is a priority in modern pathology and requires strict quality control. Cost containment has also become fundamental and auditing of all procedures must take into account both these principles. Positive controls must be routinely performed so that their positivity guarantees the appropriateness of the immunohistochemical procedure. The aim of this study is to develop a low cost (utilizing a punch biopsy-PB-tool) procedure to construct positive controls which can be integrated in the patient's tissue slide...
July 17, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28712031/human-skeletal-muscle-type-1-fibre-distribution-and-response-of-stress-sensing-proteins-along-the-titin-molecule-after-submaximal-exhaustive-exercise
#13
Satu O A Koskinen, Heikki Kyröläinen, Riina Flink, Harri P Selänne, Sheila S Gagnon, Juha P Ahtiainen, Bradley C Nindl, Maarit Lehti
Early responses of stress-sensing proteins, muscle LIM protein (MLP), ankyrin repeat proteins (Ankrd1/CARP and Ankrd2/Arpp) and muscle-specific RING finger proteins (MuRF1 and MuRF2), along the titin molecule were investigated in the present experiment after submaximal exhaustive exercise. Ten healthy men performed continuous drop jumping unilaterally on a sledge apparatus with a submaximal height until complete exhaustion. Five stress-sensing proteins were analysed by mRNA measurements from biopsies obtained immediately and 3 h after the exercise from exercised vastus lateralis muscle while control biopsies were obtained from non-exercised legs before the exercise...
July 15, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28702782/immunohistochemical-localization-of-histidine-rich-glycoprotein-in-human-skeletal-muscle-preferential-distribution-of-the-protein-at-the-sarcomeric-i-band
#14
L Mattii, L Rossi, C Ippolito, G Alì, D Martini, A Raggi, Antonietta R M Sabbatini
Histidine-rich glycoprotein (HRG) is a relatively abundant plasma protein that is synthesized by parenchymal liver cells. Using Western blot analysis and immunoperoxidase techniques, we have previously shown the presence of HRG in human skeletal muscle. This paper reports the results of immunofluorescence experiments carried out on sections of human normal skeletal muscle biopsies to investigate the subcellular localization of HRG. The HRG localization was also compared with that of skeletal muscle AMP deaminase (AMPD1), since we have previously described an association of the enzyme with the protein...
July 12, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28695336/experimentally-induced-intrauterine-growth-restriction-in-rabbits-leads-to-differential-remodelling-of-left-versus-right-ventricular-myocardial-microstructure
#15
Julia Schipke, Anna Gonzalez-Tendero, Lidia Cornejo, Alper Willführ, Bart Bijnens, Fatima Crispi, Christian Mühlfeld, Eduard Gratacós
Intrauterine growth restriction (IUGR) is associated with foetal cardiac remodelling and dysfunction together with increased risk of cardiovascular disease in adulthood. Experimental data concerning effects of IUGR on cardiomyocyte and microvascularization anatomy are inconsistent and it is unknown whether both ventricles are similarly susceptible to in utero undersupply. Foetal IUGR was induced in pregnant rabbits at 25 days of gestation by selective ligation of uteroplacental vessels. Foetal echocardiography showed systolic and diastolic dysfunction of both ventricles and body and heart weight were significantly reduced in response to IUGR...
July 10, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28681271/cell-cycle-analysis-in-the-rat-external-granular-layer-evaluated-by-several-bromodeoxyuridine-immunoperoxidase-staining-protocols
#16
Vanesa Molina, Lucía Rodríguez-Vázquez, David Owen, Oliver Valero, Joaquín Martí
An important step in bromodeoxyuridine (BrdU) immunohistochemistry is the production of single-stranded DNA to make the incorporated BrdU accessible to the antibodies. This paper examines the effect of distinct DNA denaturation pretreatments (DNase I, sodium citrate buffer, endonuclease Eco RI and exonuclease III, and HCl hydrolysis) on detection of BrdU. We found that all the methods used in the partial denaturation of DNA combined good nuclear immunostaining with acceptable tissue integrity. We also observed that these immunohistochemical protocols revealed a spatial pattern in the distribution of DNA-synthesizing cells within the cerebellar external granular layer (EGL) of 10-day-old rats, allowing us to estimate the fraction of S-phase cells...
July 6, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28664293/targeting-autophagy-to-modulate-cell-survival-a-comparative-analysis-in-cancer-normal-and-embryonic-cells
#17
Aleksandra Divac Rankov, Mila Ljujić, Marija Petrić, Dragica Radojković, Milica Pešić, Jelena Dinić
Autophagy is linked to multiple cancer-related signaling pathways, and represents a defense mechanism for cancer cells under therapeutic stress. The crosstalk between apoptosis and autophagy is essential for both tumorigenesis and embryonic development. We studied the influence of autophagy on cell survival in pro-apoptotic conditions induced by anticancer drugs in three model systems: human cancer cells (NCI-H460, COR-L23 and U87), human normal cells (HaCaT and MRC-5) and zebrafish embryos (Danio rerio). Autophagy induction with AZD2014 and tamoxifen antagonized the pro-apoptotic effect of chemotherapeutics doxorubicin and cisplatin in cell lines, while autophagy inhibition by wortmannin and chloroquine synergized the action of both anticancer agents...
June 29, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28721451/in-focus-in-hcb
#18
EDITORIAL
Douglas J Taatjes, Jürgen Roth
No abstract text is available yet for this article.
September 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28560490/laser-capture-microdissection-protocol-for-gene-expression-analysis-in-the-brain
#19
P Garrido-Gil, P Fernandez-Rodríguez, J Rodríguez-Pallares, Jose L Labandeira-Garcia
Laser capture microdissection (LCM) allows the isolation of specific cell populations from complex tissues that can be then used for gene expression studies. However, there are no reproducible protocols to study RNA in the brain and, particularly, in the substantia nigra. RNA is a very labile biomolecule that is easily degraded during manipulation. LCM studies use low amounts of material and special precautions must be taken to preserve RNA yield and integrity, which are decisive for PCR analysis. The RNA yield and/or integrity can be affected negatively by tissue manipulation, LCM process and RNA extraction...
September 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28550404/development-of-an-imaging-system-for-in-vivo-real-time-monitoring-of-neuronal-activity-in-deep-brain-of-free-moving-rats
#20
Norio Iijima, Shinji Miyamoto, Keisuke Matsumoto, Ken Takumi, Yoichi Ueta, Hitoshi Ozawa
We have newly developed a system that allows monitoring of the intensity of fluorescent signals from deep brains of rats transgenically modified to express enhanced green fluorescent protein (eGFP) via an optical fiber. One terminal of the optical fiber was connected to a blue semiconductor laser oscillator/green fluorescence detector. The other terminal was inserted into the vicinity of the eGFP-expressing neurons. Since the optical fiber was vulnerable to twisting stresses caused by animal movement, we also developed a cage in which the floor automatically turns, in response to the turning of the rat's head...
September 2017: Histochemistry and Cell Biology
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