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Histochemistry and Cell Biology

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https://www.readbyqxmd.com/read/28534071/cloning-of-helicobacter-suis-cholesterol-%C3%AE-glucosyltransferase-and-production-of-an-antibody-capable-of-detecting-it-in-formalin-fixed-paraffin-embedded-gastric-tissue-sections
#1
Masatomo Kawakubo, Kazuki Horiuchi, Hitomi Komura, Yoshiko Sato, Masayoshi Kato, Meguru Ikeyama, Mana Fukushima, Shigenori Yamada, Satoshi Ishizone, Takehisa Matsumoto, Hiroyoshi Ota, Junji Sagara, Jun Nakayama
Helicobacter suis (H. suis), formerly called Helicobacter heilmannii type 1 (H. heilmannii), is a gram-negative bacterium of the Helicobacter species. This pathogen infects the stomach of humans and animals such as dogs, cats, pigs, and rodents, the latter giving rise to zoonotic infection. Here, we generated a H. suis-specific antibody useful for immunohistochemistry with formalin-fixed, paraffin-embedded tissue sections. To do so, we began by cloning the gene encoding H. suis cholesterol α-glucosyltransferase (αCgT)...
May 22, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28523458/the-respiratory-chain-inhibitor-rotenone-affects-peroxisomal-dynamics-via-its-microtubule-destabilising-activity
#2
Josiah B Passmore, Sonia Pinho, Maria Gomez-Lazaro, Michael Schrader
Peroxisomes and mitochondria in mammalian cells are closely linked subcellular organelles, which maintain a redox-sensitive relationship. Their interplay and role in ROS signalling are supposed to impact on age-related and degenerative disorders. Whereas the generation of peroxisome-derived oxidative stress can affect mitochondrial morphology and function, little is known about the impact of mitochondria-derived oxidative stress on peroxisomes. Here, we investigated the effect of the mitochondrial complex I inhibitor rotenone on peroxisomal and mitochondrial membrane dynamics...
May 18, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28500503/expression-and-localization-of-myosin-vi-in-developing-mouse-spermatids
#3
Przemysław Zakrzewski, Robert Lenartowski, Maria Jolanta Rędowicz, Kathryn G Miller, Marta Lenartowska
Myosin VI (MVI) is a versatile actin-based motor protein that has been implicated in a variety of different cellular processes, including endo- and exocytic vesicle trafficking, Golgi morphology, and actin structure stabilization. A role for MVI in crucial actin-based processes involved in sperm maturation was demonstrated in Drosophila. Because of the prominence and importance of actin structures in mammalian spermiogenesis, we investigated whether MVI was associated with actin-mediated maturation events in mammals...
May 12, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28500502/retinoic-acid-regulates-cell-shape-and-death-of-e-fabp-fabp5-immunoreactive-septoclasts-in-the-growth-plate-cartilage-of-mice
#4
Yasuhiko Bando, Miyuki Yamamoto, Koji Sakiyama, Hide Sakashita, Fuyoko Taira, Genki Miyake, Shoichi Iseki, Yuji Owada, Osamu Amano
Septoclasts, which are mononuclear and spindle-shaped cells with many processes, have been considered to resorb the transverse septa of the growth plate (GP) cartilage at the chondro-osseous junction (COJ). We previously reported the expression of epidermal-type fatty acid-binding protein (E-FABP, FABP5) and localization of peroxisome proliferator-activated receptor (PPAR)β/δ, which mediates the cell survival or proliferation, in septoclasts. On the other hand, retinoic acid (RA) can bind to E-FABP and is stored abundantly in the GP cartilage...
May 12, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28493059/characterization-of-oatp1b3-and-oatp2b1-transporter-expression-in-the-islet-of-the-adult-human-pancreas
#5
Michelle Kim, Perri Deacon, Rommel G Tirona, Richard B Kim, Christopher L Pin, Henriette E Meyer Zu Schwabedissen, Rennian Wang, Ute I Schwarz
Organic anion-transporting polypeptides (OATPs) are membrane proteins that mediate cellular uptake of structurally diverse endogenous and exogenous compounds, including bile salts, thyroid and sex hormones, pharmacological agents, and toxins. Roles of OATPs in human liver are well established. Our recent report suggested the presence of the hepatic transporter OATP1B3 in human β cells. The aim of this study was to better characterize cellular localization and interindividual variation in OATP1B3 expression in human adult islets as a function of age, sex, and pancreatic disease, and to assess the expression of other OATPs...
May 10, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28478589/preoperative-factors-predicting-saphenous-vein-graft-occlusion-in-coronary-artery-bypass-grafting-a-multivariate-analysis
#6
Agnieszka Malinska, Zuzanna Podemska, Bartlomiej Perek, Marek Jemielity, Piotr Buczkowski, Malgorzata Grzymislawska, Patrycja Sujka-Kordowska, Michal Nowicki
Saphenous vein segments are frequently used as aortocoronary bypass grafts, particularly in patients over 65 years of age. In the majority of patients, venous grafts maintain their patency for 5-6 years; however, some become occluded within 12 months after surgery. There are some defined predictive biological factors used to assess saphenous vein graft long-term patency rates, but little is known about molecular parameters for estimating the risk of early vein occlusion. The pathogenesis of this process involves the proliferation of stem cells, as well as progenitor cells, in the graft wall...
May 6, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28478588/vessel-wall-morphology-is-equivalent-for-different-artery-types-and-localizations-of-advanced-human-aneurysms
#7
Albert Busch, Caroline Grimm, Elena Hartmann, Valentina Paloschi, Ralph Kickuth, Mariette Lengquist, Christoph Otto, Per Eriksson, Richard Kellersmann, Udo Lorenz, Lars Maegdefessel
Aneurysm formation occurs most frequently as abdominal aortic aneurysm (AAA), but is also seen in other localizations like thoracic or peripheral aneurysm. While initial mechanisms for aneurysm induction remain elusive, observations from AAA samples show transmural inflammation with proteolytic imbalance and repair mechanisms triggered by the innate immune system. However, limited knowledge exists about aneurysm pathology, especially for others than AAA. We compared 42 AAA, 15 popliteal, 3 ascending aortic, five iliac, two femoral, two brachial, one visceral and two secondary aneurysms to non-aneurysmatic controls by histologic analysis, immunohistochemistry and cytokine expression...
May 6, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28456845/in-focus-in-hcb
#8
EDITORIAL
Douglas J Taatjes, Jürgen Roth
No abstract text is available yet for this article.
April 29, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28432431/initial-cell-adhesion-of-three-cell-types-in-the-presence-and-absence-of-serum-proteins
#9
Martina Verdanova, Pavla Sauerova, Ute Hempel, Marie Hubalek Kalbacova
With the development of a wide range of new biomaterials for the sensing of different cell behaviour, it is important to consider whether the cells tested in vitro are in direct contact with the material or whether cell-biomaterial contact is mediated by an interfacial layer of proteins originating from the culture medium or from the cells themselves. Thus, this study describes the differences between the cell adhesion mediated by proteins originating from foetal bovine serum and without the presence of such proteins 2 h following cell seeding exemplarily with different cell types (an osteoblastic cell line, primary fibroblasts, and mesenchymal stem cells)...
April 21, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28429122/quantifying-golgi-structure-using-em-combining-volume-sem-and-stereology-for-higher-throughput
#10
REVIEW
Sophie Ferguson, Anna M Steyer, Terry M Mayhew, Yannick Schwab, John Milton Lucocq
Investigating organelles such as the Golgi complex depends increasingly on high-throughput quantitative morphological analyses from multiple experimental or genetic conditions. Light microscopy (LM) has been an effective tool for screening but fails to reveal fine details of Golgi structures such as vesicles, tubules and cisternae. Electron microscopy (EM) has sufficient resolution but traditional transmission EM (TEM) methods are slow and inefficient. Newer volume scanning EM (volume-SEM) methods now have the potential to speed up 3D analysis by automated sectioning and imaging...
April 20, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28421320/hepatocyte-specific-depletion-of-ubiquitin-regulatory-x-domain-containing-protein-8-accelerates-fibrosis-in-a-mouse-non-alcoholic-steatohepatitis-model
#11
Norihiro Imai, Michitaka Suzuki, Yoji Ishizu, Teiji Kuzuya, Takashi Honda, Kazuhiko Hayashi, Masatoshi Ishigami, Yoshiki Hirooka, Tetsuya Ishikawa, Hidemi Goto, Toyoshi Fujimoto
Ubiquitin regulatory X domain-containing protein 8 (UBXD8) is engaged in the degradation of lipidated apolipoprotein B in hepatocytes. We previously showed that hepatocyte-specific UBXD8-deficient mice (U8-HKO) fed a moderately high-fat diet (31 kcal % fat) showed periportal macrovesicular steatosis along with a decrease in very low-density lipoprotein secretion, but did not develop fibrosis. In the present study, we examined whether U8-HKO mice show NASH-like phenotypes when fed a very high-fat diet (60 kcal % fat)...
April 18, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28417191/mapping-of-estradiol-binding-sites-through-receptor-micro-autoradiography-in-the-endometrial-stroma-of-early-pregnant-mice
#12
Telma M T Zorn, Rodolfo R Favaro, Mauricio Soto-Suazo, Walter E Stumpf
Estradiol triggers key biological responses in the endometrium, which rely on the presence and levels of its cognate receptors on target cells. Employing the receptor micro-autoradiography (RMAR) technique, we aimed to provide a temporal and spatial map of the functional binding sites for estradiol in the mouse endometrial stroma during early pregnancy. Uterine samples from days 1.5 to 7.5 of pregnancy were collected 1 h after tritiated- (3H-) estradiol administration and prepared for RMAR analysis. Autoradiographic incorporation of 3H-thymidine (after 1-h pulse) was evaluated over the same gestational interval...
April 17, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28405806/rearrangement-of-potassium-ions-and-kv1-1-kv1-2-potassium-channels-in-regenerating-axons-following-end-to-end-neurorrhaphy-ionic-images-from-tof-sims
#13
Chiung-Hui Liu, Hung-Ming Chang, Tsung-Huan Wu, Li-You Chen, Yin-Shuo Yang, To-Jung Tseng, Wen-Chieh Liao
The voltage-gated potassium channels Kv1.1 and Kv1.2 that cluster at juxtaparanodal (JXP) regions are essential in the regulation of nerve excitability and play a critical role in axonal conduction. When demyelination occurs, Kv1.1/Kv1.2 activity increases, suppressing the membrane potential nearly to the equilibrium potential of K(+), which results in an axonal conduction blockade. The recovery of K(+)-dependent communication signals and proper clustering of Kv1.1/Kv1.2 channels at JXP regions may directly reflect nerve regeneration following peripheral nerve injury...
April 12, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28397143/tropism-intracerebral-distribution-and-transduction-efficiency-of-hiv-and-siv-based-lentiviral-vectors-after-injection-into-the-mouse-brain-a-qualitative-and-quantitative-in-vivo-study
#14
Juraj Hlavatý, Zbyněk Tonar, Matthias Renner, Sylvia Panitz, Helga Petznek, Matthias Schweizer, Silke Schüle, Björn-Philipp Kloke, Rudolf Moldzio, Kirsti Witter
Lentiviruses are suitable to transfer potential therapeutic genes into non-replicating cells such as neurons, but systematic in vivo studies on transduction of neural cells within the complete brain are missing. We analysed the distribution of transduced cells with respect to brain structure, virus tropism, numbers of transduced neurons per brain, and influence of the Vpx or Vpr accessory proteins after injection of vectors based on SIVsmmPBj, HIV-2, and HIV-1 lentiviruses into the right striatum of the mouse brain...
April 10, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28397142/mutations-in-the-tp53-gene-affected-recruitment-of-53bp1-protein-to-dna-lesions-but-level-of-53bp1-was-stable-after-%C3%AE-irradiation-that-depleted-mdc1-protein-in-specific-tp53-mutants
#15
Jana Suchánková, Soňa Legartová, Eva Ručková, Bořivoj Vojtěšek, Stanislav Kozubek, Eva Bártová
53BP1 is a very well-known protein that is recruited to DNA lesions. The focal accumulation of p53 binding protein, 53BP1, is a main feature indicating the repair of spontaneous or irradiation-induced foci (IRIF). Thus, here, we addressed the question of whether mutations in the TP53 gene, which often affect the level of p53 protein, can change the recruitment of 53BP1 to γ- or UVA-irradiated chromatin. In various TP53 mutants, we observed a distinct accumulation of 53BP1 protein to UV-induced DNA lesions: in R273C mutants, 53BP1 appeared transiently at DNA lesions, during 10-30 min after irradiation; the mutation R282W was responsible for accumulation of 53BP1 immediately after UVA-damage; and in L194F mutants, the first appearance of 53BP1 protein at the lesions occurred during 60-70 min...
April 10, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28397141/differentiation-dependent-rearrangements-of-actin-filaments-and-microtubules-hinder-apical-endocytosis-in-urothelial-cells
#16
Larisa Tratnjek, Rok Romih, Mateja Erdani Kreft
During differentiation, superficial urothelial cells (UCs) of the urinary bladder form the apical surface, which is almost entirely covered by urothelial plaques containing densely packed uroplakin particles. These urothelial plaques are the main structural components of the blood-urine permeability barrier in the urinary bladder. We have shown previously that endocytosis from the apical plasma membrane decreases during urothelial cell differentiation. Here, we investigated the role of actin filament and microtubule rearrangements in apical endocytosis of differentiating UCs cells using hyperplastic and normoplastic porcine urothelial models...
April 10, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28391396/%C3%A2-in-focus-in-hcb
#17
EDITORIAL
Douglas J Taatjes, Jürgen Roth
No abstract text is available yet for this article.
April 8, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28386635/vasa-expression-suggests-shared-germ-line-dynamics-in-bivalve-molluscs
#18
Liliana Milani, Andrea Pecci, Fabrizio Ghiselli, Marco Passamonti, Simone Bettini, Valeria Franceschini, Maria Gabriella Maurizii
Germ line segregation can occur during embryogenesis or after embryogenesis completion, with multipotent cells able to give rise to both germ and somatic cells in the developing juvenile or even in adulthood. These undifferentiated cells, in some animals, are self-renewing stem cells. In all these cell lineages, the same set of genes, among which vasa, appears to be expressed. We traced VASA expression during the peculiar gonad rebuilding of bivalves to verify its presence from undifferentiated germ cells to mature gametes in an animal taxon in which the mechanism of germ line establishment is still under investigation...
April 6, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28365860/low-molecular-weight-hyaluronan-induces-migration-of-human-choriocarcinoma-jeg-3-cells-mediated-by-rhamm-as-well-as-by-pi3k-and-mapk-pathways
#19
Marilina Mascaró, Matías A Pibuel, Silvina L Lompardía, Mariangeles Díaz, Elsa Zotta, Maria I Bianconi, Néstor Lago, Silvina Otero, Gustavo Jankilevich, Elida Alvarez, Silvia E Hajos
Hyaluronan (HA) is the major glycosaminoglycan present in the extracellular matrix. It is produced by some tumours and promotes proliferation, differentiation and migration among others cellular processes. Gestational trophoblastic disease (GTD) is composed by non-tumour entities, such as hydatidiform mole (HM), which is the most common type of GTD and also malignant entities such as choriocarcinoma (CC) and placental site trophoblastic tumour (PSTT), being CC the most aggressive tumour. Although there is a growing understanding of GTD biology, the role of HA in the pathogenesis of this group of diseases remains largely unknown...
April 1, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28365859/epithelial-mesenchymal-transition-and-collective-and-individual-cell-migration-regulate-epithelial-changes-in-the-amikacin-damaged-organ-of-corti
#20
Sabine Ladrech, Michel Eybalin, Jean-Luc Puel, Marc Lenoir
Characterizing the microenvironment of a damaged organ of Corti and identifying the basic mechanisms involved in subsequent epithelial reorganization are critical for improving the outcome of clinical therapies. In this context, we studied the expression of a variety of cell markers related to cell shape, cell adhesion and cell plasticity in the rat organ of Corti poisoned with amikacin. Our results indicate that, after severe outer hair cell losses, the cytoarchitectural reorganization of the organ of Corti implicates epithelial-mesenchymal transition mechanisms and involves both collective and individual cell migratory processes...
April 1, 2017: Histochemistry and Cell Biology
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