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Histochemistry and Cell Biology

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https://www.readbyqxmd.com/read/28815335/in-focus-in-hcb
#1
EDITORIAL
Douglas J Taatjes, Jürgen Roth
No abstract text is available yet for this article.
August 17, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28755317/early-inflammatory-profiling-of-schwannoma-cells-induced-by-lipopolysaccharide
#2
Marcela Kohoutková, Andrea Korimová, Václav Brázda, Jiří Kohoutek, Petr Dubový
Inflammatory profiling of Schwann cells manifested as an upregulation of cytokines is present after traumatic or disease injury of the peripheral nerves. Inflammatory activation of Schwann cells via Toll-like receptors (TLRs) can be triggered by exogenous pathological molecules or endogenous ligands produced during Wallerian degeneration. We investigated the early period of inflammatory reactions by following the levels of TLR4, NFκB, IL-1β, pSTAT3, and IL-6 proteins after LPS treatment of RT4 schwannoma cells under in vitro conditions...
July 28, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28755316/deficiency-of-a-membrane-skeletal-protein-4-1g-results-in-myelin-abnormalities-in-the-peripheral-nervous-system
#3
Yurika Saitoh, Nobuhiko Ohno, Junji Yamauchi, Takeharu Sakamoto, Nobuo Terada
We previously demonstrated that a membrane skeletal molecular complex, 4.1G-membrane palmitoylated protein 6 (MPP6)-cell adhesion molecule 4, is incorporated in Schwann cells in the peripheral nervous system (PNS). In this study, we evaluated motor activity and myelin ultrastructures in 4.1G-deficient (-/-) mice. When suspended by the tail, aged 4.1G(-/-) mice displayed spastic leg extension, especially after overwork. Motor-conduction velocity in 4.1G(-/-) mice was slower than that in wild-type mice. Using electron microscopy, 4...
July 28, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28748255/cellular-and-subcellular-localization-of-adp-ribosylation-factor-6-in-mouse-peripheral-tissues
#4
Osamu Katsumata, Momoko Mori, Yusuke Sawane, Tomoko Niimura, Akiko Ito, Hirotsugu Okamoto, Masahiro Fukaya, Hiroyuki Sakagami
ADP-ribosylation factor 6 (Arf6) is a small GTPase that regulates endosomal trafficking and actin cytoskeleton remodeling. In the present study, we comprehensively examined the cellular and subcellular localization of Arf6 in adult mouse peripheral tissues by immunofluorescence and immunoelectron microscopy using the heat-induced antigen retrieval method with Tris-EDTA buffer (pH 9.0). Marked immunolabeling of Arf6 was observed particularly in epithelial cells of several tissues including the esophagus, stomach, small and large intestines, trachea, kidney, epididymis, oviduct, and uterus...
July 26, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28721450/smad3-and-bmal1-regulate-p21-and-s100a4-expression-in-myocardial-stromal-fibroblasts-via-tnf-%C3%AE
#5
Fuyuki Sato, Akira Kohsaka, Kana Takahashi, Saki Otao, Yusuke Kitada, Yoshiyuki Iwasaki, Yasuteru Muragaki
Bmal1, a clock gene, is associated with depression, hypertrophy, metabolic syndrome and diabetes. Smad3, which is involved in the TGF-β signaling pathway, plays an important role in the regulation of tumor progression, fibrosis, obesity and diabetes. Our previous report showed that Smad3 has circadian expression in mouse livers. In the current study, we focused on the heart, especially on the myocardial stromal fibroblasts because the roles of Bmal1 and Smad3 in this tissue are poorly understood. Bmal1 and Smad3 have circadian expression in mouse hearts, and their circadian expression patterns were similar...
July 18, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28714056/quality-control-in-diagnostic-immunohistochemistry-integrated-on-slide-positive-controls
#6
A Bragoni, A Gambella, S Pigozzi, M Grigolini, R Fiocca, L Mastracci, F Grillo
Standardization in immunohistochemistry is a priority in modern pathology and requires strict quality control. Cost containment has also become fundamental and auditing of all procedures must take into account both these principles. Positive controls must be routinely performed so that their positivity guarantees the appropriateness of the immunohistochemical procedure. The aim of this study is to develop a low cost (utilizing a punch biopsy-PB-tool) procedure to construct positive controls which can be integrated in the patient's tissue slide...
July 17, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28712031/human-skeletal-muscle-type-1-fibre-distribution-and-response-of-stress-sensing-proteins-along-the-titin-molecule-after-submaximal-exhaustive-exercise
#7
Satu O A Koskinen, Heikki Kyröläinen, Riina Flink, Harri P Selänne, Sheila S Gagnon, Juha P Ahtiainen, Bradley C Nindl, Maarit Lehti
Early responses of stress-sensing proteins, muscle LIM protein (MLP), ankyrin repeat proteins (Ankrd1/CARP and Ankrd2/Arpp) and muscle-specific RING finger proteins (MuRF1 and MuRF2), along the titin molecule were investigated in the present experiment after submaximal exhaustive exercise. Ten healthy men performed continuous drop jumping unilaterally on a sledge apparatus with a submaximal height until complete exhaustion. Five stress-sensing proteins were analysed by mRNA measurements from biopsies obtained immediately and 3 h after the exercise from exercised vastus lateralis muscle while control biopsies were obtained from non-exercised legs before the exercise...
July 15, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28702782/immunohistochemical-localization-of-histidine-rich-glycoprotein-in-human-skeletal-muscle-preferential-distribution-of-the-protein-at-the-sarcomeric-i-band
#8
L Mattii, L Rossi, C Ippolito, G Alì, D Martini, A Raggi, Antonietta R M Sabbatini
Histidine-rich glycoprotein (HRG) is a relatively abundant plasma protein that is synthesized by parenchymal liver cells. Using Western blot analysis and immunoperoxidase techniques, we have previously shown the presence of HRG in human skeletal muscle. This paper reports the results of immunofluorescence experiments carried out on sections of human normal skeletal muscle biopsies to investigate the subcellular localization of HRG. The HRG localization was also compared with that of skeletal muscle AMP deaminase (AMPD1), since we have previously described an association of the enzyme with the protein...
July 12, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28695336/experimentally-induced-intrauterine-growth-restriction-in-rabbits-leads-to-differential-remodelling-of-left-versus-right-ventricular-myocardial-microstructure
#9
Julia Schipke, Anna Gonzalez-Tendero, Lidia Cornejo, Alper Willführ, Bart Bijnens, Fatima Crispi, Christian Mühlfeld, Eduard Gratacós
Intrauterine growth restriction (IUGR) is associated with foetal cardiac remodelling and dysfunction together with increased risk of cardiovascular disease in adulthood. Experimental data concerning effects of IUGR on cardiomyocyte and microvascularization anatomy are inconsistent and it is unknown whether both ventricles are similarly susceptible to in utero undersupply. Foetal IUGR was induced in pregnant rabbits at 25 days of gestation by selective ligation of uteroplacental vessels. Foetal echocardiography showed systolic and diastolic dysfunction of both ventricles and body and heart weight were significantly reduced in response to IUGR...
July 10, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28681271/cell-cycle-analysis-in-the-rat-external-granular-layer-evaluated-by-several-bromodeoxyuridine-immunoperoxidase-staining-protocols
#10
Vanesa Molina, Lucía Rodríguez-Vázquez, David Owen, Oliver Valero, Joaquín Martí
An important step in bromodeoxyuridine (BrdU) immunohistochemistry is the production of single-stranded DNA to make the incorporated BrdU accessible to the antibodies. This paper examines the effect of distinct DNA denaturation pretreatments (DNase I, sodium citrate buffer, endonuclease Eco RI and exonuclease III, and HCl hydrolysis) on detection of BrdU. We found that all the methods used in the partial denaturation of DNA combined good nuclear immunostaining with acceptable tissue integrity. We also observed that these immunohistochemical protocols revealed a spatial pattern in the distribution of DNA-synthesizing cells within the cerebellar external granular layer (EGL) of 10-day-old rats, allowing us to estimate the fraction of S-phase cells...
July 6, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28664293/targeting-autophagy-to-modulate-cell-survival-a-comparative-analysis-in-cancer-normal-and-embryonic-cells
#11
Aleksandra Divac Rankov, Mila Ljujić, Marija Petrić, Dragica Radojković, Milica Pešić, Jelena Dinić
Autophagy is linked to multiple cancer-related signaling pathways, and represents a defense mechanism for cancer cells under therapeutic stress. The crosstalk between apoptosis and autophagy is essential for both tumorigenesis and embryonic development. We studied the influence of autophagy on cell survival in pro-apoptotic conditions induced by anticancer drugs in three model systems: human cancer cells (NCI-H460, COR-L23 and U87), human normal cells (HaCaT and MRC-5) and zebrafish embryos (Danio rerio). Autophagy induction with AZD2014 and tamoxifen antagonized the pro-apoptotic effect of chemotherapeutics doxorubicin and cisplatin in cell lines, while autophagy inhibition by wortmannin and chloroquine synergized the action of both anticancer agents...
June 29, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28620865/involvement-of-atm-and-trp53-in-neural-cell-loss-due-to-terf2-inactivation-during-mouse-brain-development
#12
Jusik Kim, Inseo Choi, Youngsoo Lee
Maintenance of genomic integrity is one of the critical features for proper neurodevelopment and inhibition of neurological diseases. The signals from both ATM and ATR to TP53 are well-known mechanisms to remove neural cells with DNA damage during neurogenesis. Here we examined the involvement of Atm and Atr in genomic instability due to Terf2 inactivation during mouse brain development. Selective inactivation of Terf2 in neural progenitors induced apoptosis, resulting in a complete loss of the brain structure...
June 15, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28620864/adipose-tissue-derived-stromal-cells-adsc-express-oligodendrocyte-and-myelin-markers-but-they-do-not-function-as-oligodendrocytes
#13
Lara Vellosillo, Maria Paz Muñoz, Carlos Luis Paíno
Mesenchymal cells cultured from the vasculo-stromal fraction of adipose tissue (ADSC) show adult stem cell characteristics and several groups have claimed generating neural cells from them. However, we have observed that many markers commonly used for the identification of neural cells are spontaneously expressed by ADSC in culture. In the present study, we have examined the expression of characteristic oligodendrocyte molecules in cultured ADSC, aiming to test if myelinating cells could be generated from accessible non-neural adult tissues...
June 15, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28612087/effects-of-vascular-formation-during-alveolar-bone-process-morphogenesis-in-mice
#14
Seo-Young An, Ye-Ji Lee, Sanjiv Neupane, Jong-Hwa Jun, Ji-Youn Kim, Youngkyun Lee, Karp-Shik Choi, Chang-Hyeon An, Jo-Young Suh, Hong-In Shin, Wern-Joo Sohn, Jae-Young Kim
The alveolar bone process is the thickened ridge of bone that bears the teeth and is known to have dynamic functional interactions with surrounding tissues. However, the detailed morphological changes that occur during alveolar bone process development and the underlying molecular mechanisms behind this morphogenesis have not been elucidated. In this study, we examined the detailed morphological changes of the alveolar bone process during mouse development using HE and MTC staining. In addition, we evaluated the precise localization pattern of various signaling molecules involved in blood vessel formation including CD31, α-SMA, VEGF, periostin, and TGF-β...
June 13, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28597061/progressive-accumulation-of-autofluorescent-granules-in-macrophages-in-rat-striatum-after-systemic-3-nitropropionic-acid-a-correlative-light-and-electron-microscopic-study
#15
Tae-Ryong Riew, Hong Lim Kim, Jeong-Heon Choi, Xuyan Jin, Yoo-Jin Shin, Mun-Yong Lee
A variety of tissue biomolecules and intracellular structures are known to be autofluorescent. However, autofluorescent signals in brain tissues often confound analysis of the fluorescent markers used for immunohistochemistry. While investigating tissue and cellular pathologies induced by 3-nitropropionic acid, a mitochondrial toxin selective for striatal neurons, we encountered many autofluorescent signals confined to the lesion core. These structures were excited by blue (wavelength = 488 nm) and yellow-orange (555 nm), but not by red (639 nm) or violet (405 nm) lasers, indicating that this autofluorescence overlaps with the emission spectra of commonly used fluorophores...
June 9, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28589288/in-focus-in-hcb
#16
EDITORIAL
Douglas J Taatjes, Jürgen Roth
No abstract text is available yet for this article.
June 6, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28540429/identification-of-markers-for-quiescent-pancreatic-stellate-cells-in-the-normal-human-pancreas
#17
Michael Friberg Bruun Nielsen, Michael Bau Mortensen, Sönke Detlefsen
Pancreatic stellate cells (PSCs) play a central role as source of fibrogenic cells in pancreatic cancer and chronic pancreatitis. In contrast to quiescent hepatic stellate cells (qHSCs), a specific marker for quiescent PSCs (qPSCs) that can be used in formalin-fixed and paraffin embedded (FFPE) normal human pancreatic tissue has not been identified. The aim of this study was to identify a marker enabling the identification of qPSCs in normal human FFPE pancreatic tissue. Immunohistochemical (IHC), double-IHC, immunofluorescence (IF) and double-IF analyses were carried out using a tissue microarray consisting of cores with normal human pancreatic tissue...
May 25, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28536901/increased-epithelial-free-areas-in-thymuses-with-altered-ephb-mediated-thymocyte-thymic-epithelial-cell-interactions
#18
Javier García-Ceca, Sara Montero-Herradón, David Alfaro, Agustín G Zapata
Epithelial-free areas, present in both thymic cortex and medulla, have been studied in WT and EphB-deficient mice that have important alterations in the development of thymic epithelium due to the lack of proper thymocyte-thymic epithelial cell interactions. In both WT and mutant thymuses, the number and size of epithelial-free areas are significantly larger in the medulla than in the cortex. The two parameters show a reverse correlation: low numbers of these areas course with large epithelial-free areas and vice versa...
May 24, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28534071/cloning-of-helicobacter-suis-cholesterol-%C3%AE-glucosyltransferase-and-production-of-an-antibody-capable-of-detecting-it-in-formalin-fixed-paraffin-embedded-gastric-tissue-sections
#19
Masatomo Kawakubo, Kazuki Horiuchi, Hitomi Komura, Yoshiko Sato, Masayoshi Kato, Meguru Ikeyama, Mana Fukushima, Shigenori Yamada, Satoshi Ishizone, Takehisa Matsumoto, Hiroyoshi Ota, Junji Sagara, Jun Nakayama
Helicobacter suis (H. suis), formerly called Helicobacter heilmannii type 1 (H. heilmannii), is a gram-negative bacterium of the Helicobacter species. This pathogen infects the stomach of humans and animals such as dogs, cats, pigs, and rodents, the latter giving rise to zoonotic infection. Here, we generated a H. suis-specific antibody useful for immunohistochemistry with formalin-fixed, paraffin-embedded tissue sections. To do so, we began by cloning the gene encoding H. suis cholesterol α-glucosyltransferase (αCgT)...
May 22, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28721451/in-focus-in-hcb
#20
EDITORIAL
Douglas J Taatjes, Jürgen Roth
No abstract text is available yet for this article.
September 2017: Histochemistry and Cell Biology
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