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Histochemistry and Cell Biology

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https://www.readbyqxmd.com/read/29151145/pendimethalin-induces-oxidative-stress-dna-damage-and-mitochondrial-dysfunction-to-trigger-apoptosis-in-human-lymphocytes-and-rat-bone-marrow-cells
#1
Sabiha M Ansari, Quaiser Saquib, Sabry M Attia, Eslam M Abdel-Salam, Hend A Alwathnani, Mohammad Faisal, Abdulrahman A Alatar, Abdulaziz A Al-Khedhairy, Javed Musarrat
Pendimethalin (PM) is a dinitroaniline herbicide extensively applied against the annual grasses and broad-leaved weeds. There is no report available on PM-induced low-dose genotoxicity in human primary cells and in vivo test models. Such data gap has prompted us to evaluate the genotoxic potential of PM in human lymphocytes and rats. PM selectively binds in the minor groove of DNA by forming covalent bonds with G and C nitrogenous bases, as well as with the ribose sugar. PM induces micronucleus formation (MN) in human lymphocytes, indicating its clastogenic potential...
November 18, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/29143117/the-expression-of-ve-cadherin-in-breast-cancer-cells-modulates-cell-dynamics-as-a-function-of-tumor-differentiation-and-promotes-tumor-endothelial-cell-interactions
#2
Maryam Rezaei, Jiahui Cao, Katrin Friedrich, Björn Kemper, Oliver Brendel, Marianne Grosser, Manuela Adrian, Gustavo Baretton, Georg Breier, Hans-Joachim Schnittler
The cadherin switch has profound consequences on cancer invasion and metastasis. The endothelial-specific vascular endothelial cadherin (VE-cadherin) has been demonstrated in diverse cancer types including breast cancer and is supposed to modulate tumor progression and metastasis, but underlying mechanisms need to be better understood. First, we evaluated VE-cadherin expression by tissue microarray in 392 cases of breast cancer tumors and found a diverse expression and distribution of VE-cadherin. Experimental expression of fluorescence-tagged VE-cadherin (VE-EGFP) in undifferentiated, fibroblastoid and E-cadherin-negative MDA-231 (MDA-VE-EGFP) as well as in differentiated E-cadherin-positive MCF-7 human breast cancer cell lines (MCF-VE-EGFP), respectively, displayed differentiation-dependent functional differences...
November 15, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/29134302/nuclear-organization-during-in-vitro-differentiation-of-porcine-mesenchymal-stem-cells-mscs-into-adipocytes
#3
Joanna Stachecka, Agnieszka Walczak, Beata Kociucka, Błażej Ruszczycki, Grzegorz Wilczyński, Izabela Szczerbal
Differentiation of progenitor cells into adipocytes is accompanied by remarkable changes in cell morphology, cytoskeletal organization, and gene expression profile. Mature adipocytes are filled with a large lipid droplet and the nucleus tends to move to the cell periphery. It was hypothesized that the differentiation process is also associated with changes of nuclear organization. The aim of this study was to determine the number and distribution of selected components of nuclear architecture during porcine in vitro adipogenesis...
November 13, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/29134301/prostaglandin-e2-receptor-ep1-in-healthy-and-diseased-human-endometrium
#4
Junyan Zhu, Doris Mayr, Christina Kuhn, Sven Mahner, Udo Jeschke, Viktoria von Schönfeldt
Prostaglandin E2 (PGE2) is well described to be associated with both endometrial functions and disorders. The primary aim of this study was to explore the underlying mechanisms that affect the growth and function of endometrial epithelium and stroma by assessing the staining intensity of PGE2 receptors (EP) in healthy endometrium across the menstrual cycle and in pathological endometrium, such as ovarian endometriosis and endometrial cancer. We retrospectively analyzed the EPs staining intensity in human nonpregnant endometrium throughout the menstrual cycle by immunohistochemistry and further focused on EP1 (n = 42)...
November 13, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/29134300/freeze-fracture-new-avenues-for-the-ultrastructural-analysis-of-cells-in-vitro
#5
REVIEW
Carola Meier, Anja Beckmann
The ultrastructural analysis of biological membranes by freeze fracture has a 60-year tradition. In this review, we summarize the benefits of the freeze-fracture technique and review special structures analyzed by freeze fracture and by combined freeze-fracture replica immunogold labeling (FRIL) of cell cultures. In principle, every cellular membrane whether of cell suspensions, mono- or bilayers of cell cultures can be analyzed in freeze fracture. The combination of freeze fracture and immunogold labeling of the replica allows the ultrastructural identification of protein assemblies in combination with the molecular identification of their constituent proteins using specific antibodies...
November 13, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/29119258/in-focus-in-hcb
#6
EDITORIAL
Douglas J Taatjes, Jürgen Roth
No abstract text is available yet for this article.
November 9, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/29052755/validation-of-an-easily-applicable-three-dimensional-immunohistochemical-imaging-method-for-a-mouse-brain-using-conventional-confocal-microscopy
#7
Tetsuhiro Kakimoto
Histological analysis has been largely confined to two-dimensional analysis of thin tissue sections, hampering detailed understanding of three-dimensional cellular distribution in biological tissues. Tissue optical clearing methods enable three-dimensional histological analysis by rendering tissues transparent and suitable for microscopic detection of the fluorescent signals inside. Despite their great potential in histological research, the tissue clearing methods are not readily accessible to many researchers because of hazardous chemicals, complicated protocols and advanced microscopy...
October 20, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/29052754/development-of-a-method-for-the-detection-of-polystyrene-microplastics-in-paraffin-embedded-histological-sections
#8
Cátia Gonçalves, Marta Martins, Maria H Costa, Pedro M Costa
The concerns about the presence of microplastics (MPs) in marine ecosystems have widely increased in the past years. This is reflected in a growing number of studies addressing the effects of exposure to these materials in indigenous, farmed and even laboratory marine animals subjected to toxicity-oriented bioassays. There have been, however, many constraints in the detection of MPs in biological tissues, as routine histological techniques tend to degrade these materials, which are especially sensitive to organic solvents...
October 20, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28990117/expression-of-matrix-metalloproteinase-12-is-highly-specific-for-non-proliferating-invasive-trophoblasts-in-the-first-trimester-and-temporally-regulated-by-oxygen-dependent-mechanisms-including-hif-1a
#9
Ursula Hiden, Christian P Eyth, Alejandro Majali-Martinez, Gernot Desoye, Carmen Tam-Amersdorfer, Berthold Huppertz, Nassim Ghaffari Tabrizi-Wizsy
During first trimester pregnancy, trophoblast cells invade from the placenta into the maternal decidua where they anchor the placenta and remodel luminal structures like spiral arteries. This process depends on proteases secreted by invading trophoblasts, which degrade extracellular matrix (ECM). We here aimed to identify proteases particularly important for trophoblast invasion. We generated a list of proteases capable of degrading decidual ECM and trophoblast integrins using MEROPS database and compared expression of these proteases between primary trophoblasts isolated from first trimester placenta (FT, n = 3), representing an invasive phenotype, vs trophoblasts isolated from term pregnancy (TT, n = 3), representing a non-invasive trophoblast phenotype...
October 9, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28983690/subcellular-localization-and-characterization-of-estrogenic-pathway-regulators-and-mediators-in-atlantic-salmon-spermatozoal-cells
#10
Kristian R von Schalburg, Brent E Gowen, Jong S Leong, Eric B Rondeau, William S Davidson, Ben F Koop
Much progress has been made regarding our understanding of aromatase regulation, estrogen synthesis partitioning and communication between the germinal and somatic compartments of the differentiating gonad. We now know that most of the enzymatic and signaling apparatus required for steroidogenesis is endogenously expressed within germ cells. However, less is known about the expression and localization of steroidogenic components within mature spermatozoa. We have assembled a sperm library presenting 197,015 putative transcripts...
October 5, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28975414/high-level-of-ctp-synthase-induces-formation-of-cytoophidia-in-cortical-neurons-and-impairs-corticogenesis
#11
Xuzhao Li, Jiongfang Xie, Maofang Hei, Jianli Tang, Yanqing Wang, Eckart Förster, Shanting Zhao
De novo synthesis of the nucleotide CTP is catalyzed by the essential pyrimidine biosynthesis enzyme CTP synthase (CTPs), which forms large-scale filamentous structures consisting of CTPs termed cytoophidia in prokaryotes and in eukaryotes. Recent studies have shown that cytoophidia are abundant in neuroepithelial stem cells in Drosophila optic lobes and that overexpression of CTPs impairs optic lobe development. Whether CTPs and cytoophidia also play a role in the development of the mammalian cortex remains elusive...
October 3, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28875375/germ-cell-line-during-the-seasonal-sexual-rest-of-clams-finding-niches-of-cells-for-gonad-renewal
#12
Liliana Milani, Andrea Pecci, Fabrizio Ghiselli, Marco Passamonti, Maurizio Lazzari, Valeria Franceschini, Maria Gabriella Maurizii
Reconstitution and renewal of tissues are key topics in developmental biology. In this brief work, we analyzed the wintry spent phase of the reproductive cycle in the Manila clam Ruditapes philippinarum (Bivalvia, Veneridae) in order to study the gonad rebuilding that in this species occurs at the beginning of the warmer months. We labeled VASA homolog protein-a germ cell marker-and compared the histological observations of the spent phase with those of the previously analyzed gametogenic phase. In R. philippinarum, during the reproductive season, most of the body mass is represented by sack-like structures (acini) full of developing gametes...
September 5, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28936549/in-focus-in-hcb
#13
EDITORIAL
Douglas J Taatjes, Jürgen Roth
No abstract text is available yet for this article.
November 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28714056/quality-control-in-diagnostic-immunohistochemistry-integrated-on-slide-positive-controls
#14
A Bragoni, A Gambella, S Pigozzi, M Grigolini, R Fiocca, L Mastracci, F Grillo
Standardization in immunohistochemistry is a priority in modern pathology and requires strict quality control. Cost containment has also become fundamental and auditing of all procedures must take into account both these principles. Positive controls must be routinely performed so that their positivity guarantees the appropriateness of the immunohistochemical procedure. The aim of this study is to develop a low cost (utilizing a punch biopsy-PB-tool) procedure to construct positive controls which can be integrated in the patient's tissue slide...
November 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28712031/human-skeletal-muscle-type-1-fibre-distribution-and-response-of-stress-sensing-proteins-along-the-titin-molecule-after-submaximal-exhaustive-exercise
#15
Satu O A Koskinen, Heikki Kyröläinen, Riina Flink, Harri P Selänne, Sheila S Gagnon, Juha P Ahtiainen, Bradley C Nindl, Maarit Lehti
Early responses of stress-sensing proteins, muscle LIM protein (MLP), ankyrin repeat proteins (Ankrd1/CARP and Ankrd2/Arpp) and muscle-specific RING finger proteins (MuRF1 and MuRF2), along the titin molecule were investigated in the present experiment after submaximal exhaustive exercise. Ten healthy men performed continuous drop jumping unilaterally on a sledge apparatus with a submaximal height until complete exhaustion. Five stress-sensing proteins were analysed by mRNA measurements from biopsies obtained immediately and 3 h after the exercise from exercised vastus lateralis muscle while control biopsies were obtained from non-exercised legs before the exercise...
November 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28695336/experimentally-induced-intrauterine-growth-restriction-in-rabbits-leads-to-differential-remodelling-of-left-versus-right-ventricular-myocardial-microstructure
#16
Julia Schipke, Anna Gonzalez-Tendero, Lidia Cornejo, Alper Willführ, Bart Bijnens, Fatima Crispi, Christian Mühlfeld, Eduard Gratacós
Intrauterine growth restriction (IUGR) is associated with foetal cardiac remodelling and dysfunction together with increased risk of cardiovascular disease in adulthood. Experimental data concerning effects of IUGR on cardiomyocyte and microvascularization anatomy are inconsistent and it is unknown whether both ventricles are similarly susceptible to in utero undersupply. Foetal IUGR was induced in pregnant rabbits at 25 days of gestation by selective ligation of uteroplacental vessels. Foetal echocardiography showed systolic and diastolic dysfunction of both ventricles and body and heart weight were significantly reduced in response to IUGR...
November 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28681271/cell-cycle-analysis-in-the-rat-external-granular-layer-evaluated-by-several-bromodeoxyuridine-immunoperoxidase-staining-protocols
#17
Vanesa Molina, Lucía Rodríguez-Vázquez, David Owen, Oliver Valero, Joaquín Martí
An important step in bromodeoxyuridine (BrdU) immunohistochemistry is the production of single-stranded DNA to make the incorporated BrdU accessible to the antibodies. This paper examines the effect of distinct DNA denaturation pretreatments (DNase I, sodium citrate buffer, endonuclease Eco RI and exonuclease III, and HCl hydrolysis) on detection of BrdU. We found that all the methods used in the partial denaturation of DNA combined good nuclear immunostaining with acceptable tissue integrity. We also observed that these immunohistochemical protocols revealed a spatial pattern in the distribution of DNA-synthesizing cells within the cerebellar external granular layer (EGL) of 10-day-old rats, allowing us to estimate the fraction of S-phase cells...
November 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28664293/targeting-autophagy-to-modulate-cell-survival-a-comparative-analysis-in-cancer-normal-and-embryonic-cells
#18
COMPARATIVE STUDY
Aleksandra Divac Rankov, Mila Ljujić, Marija Petrić, Dragica Radojković, Milica Pešić, Jelena Dinić
Autophagy is linked to multiple cancer-related signaling pathways, and represents a defense mechanism for cancer cells under therapeutic stress. The crosstalk between apoptosis and autophagy is essential for both tumorigenesis and embryonic development. We studied the influence of autophagy on cell survival in pro-apoptotic conditions induced by anticancer drugs in three model systems: human cancer cells (NCI-H460, COR-L23 and U87), human normal cells (HaCaT and MRC-5) and zebrafish embryos (Danio rerio). Autophagy induction with AZD2014 and tamoxifen antagonized the pro-apoptotic effect of chemotherapeutics doxorubicin and cisplatin in cell lines, while autophagy inhibition by wortmannin and chloroquine synergized the action of both anticancer agents...
November 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28620865/involvement-of-atm-and-trp53-in-neural-cell-loss-due-to-terf2-inactivation-during-mouse-brain-development
#19
Jusik Kim, Inseo Choi, Youngsoo Lee
Maintenance of genomic integrity is one of the critical features for proper neurodevelopment and inhibition of neurological diseases. The signals from both ATM and ATR to TP53 are well-known mechanisms to remove neural cells with DNA damage during neurogenesis. Here we examined the involvement of Atm and Atr in genomic instability due to Terf2 inactivation during mouse brain development. Selective inactivation of Terf2 in neural progenitors induced apoptosis, resulting in a complete loss of the brain structure...
November 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28620864/adipose-tissue-derived-stromal-cells-adsc-express-oligodendrocyte-and-myelin-markers-but-they-do-not-function-as-oligodendrocytes
#20
Lara Vellosillo, Maria Paz Muñoz, Carlos Luis Paíno
Mesenchymal cells cultured from the vasculo-stromal fraction of adipose tissue (ADSC) show adult stem cell characteristics and several groups have claimed generating neural cells from them. However, we have observed that many markers commonly used for the identification of neural cells are spontaneously expressed by ADSC in culture. In the present study, we have examined the expression of characteristic oligodendrocyte molecules in cultured ADSC, aiming to test if myelinating cells could be generated from accessible non-neural adult tissues...
November 2017: Histochemistry and Cell Biology
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