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Fish & Shellfish Immunology

Zhou Zheng, Wenning Xu, Jude Juventus Aweya, Mingqi Zhong, Shangjie Liu, Jingsheng Lun, Jiehui Chen, Yueling Zhang
Shrimps, which mainly rely on their innate immune system to response to infectious pathogens, have clottable proteins as an important component of this system. While transglutaminases (TGase) are found in Litopenaeus vannamei and constitute part of the coagulation system, the specific immune-related roles played by its functional domains in the immunoregulation of shrimp has not been well understood. In the present study, we report that the Ig-like domain of L. vannamei transglutaminase (TGase-C) is the main immune-related domain among the three functional domains, as it had higher bacterial agglutinative activity against Vibrio parahaemolyticus and Streptococcus iniae...
July 14, 2018: Fish & Shellfish Immunology
Shu-Ying Feng, Gao-Feng Liang, Zheng-Shun Xu, Ai-Fang Li, Jing-Xia Du, Guan-Nan Song, Shai-Yu Ren, Yu-Lin Yang, Guang-Long Jiang
Currently, white spot syndrome virus (WSSV) is one of the most serious pathogens that impacts shrimp farming around the world. A WSSV vaccine provides a significant protective benefit to the host shrimp. Although various types of vaccines against WSSV have emerged, the immune effects among them were not compared, and it remains unclear which type of vaccine has the strongest protective effect. Meanwhile, due to the lack of effective routes of administration and immunization programs, WSSV vaccines have been greatly limited in the actual shrimp farming...
July 13, 2018: Fish & Shellfish Immunology
Yan-Wei Li, Rui Han, Jiu-Le Wang, Man Yang, Xue-Ming Dan, An-Xing Li
Interleukin-1 receptor-associated kinase-3 (IRAK-3) is a unique IRAK family member, which negatively regulates the TLR-mediated immune response in mammals. However, the function of IRAK-3 remains to be elucidated in fish. In the present study, an IRAK-3 cDNA sequence (EcIRAK-3) with an ORF of 1776 bp encoding 591 amino acids was identified in the orange-spotted grouper (Epinephelus coioides). Sequence analysis indicated that EcIRAK-3 shared the conserved structure characteristics and functional sites of vertebrate IRAK-3, and has a high sequence identity and phylogenetic relationship with that of other fish species...
July 13, 2018: Fish & Shellfish Immunology
Pengfei Chu, Libo He, Yangyang Li, Rong Huang, Lanjie Liao, Yongming Li, Zuoyan Zhu, Yaping Wang
The nucleotide-binding domain and leucine-rich-repeat-containing (NLR) proteins regulate innate immunity. Although the positive regulatory impact of NLRs is clear, their inhibitory roles are not well defined. In the present study, the NLR family gene NLRX1 from grass carp (Ctenopharyngodon idella) was cloned and characterised. NLRX1 was widely expressed in all tissues examined, albeit at varying levels. After exposure to the grass carp reovirus (GCRV), NLRX1 mRNA expression levels were altered in immune organs, and dramatically altered in liver...
July 13, 2018: Fish & Shellfish Immunology
Weiming Sun, Jixing Feng
Long noncoding RNAs (lncRNAs) may play widespread roles in various biological processes. However, systematic profiles of lncRNAs in the biological responses of Pacific Oyster (Crassostrea gigas) to pathogen infection have not yet been demonstrated. Here, we have conducted an exhaustive comparative transcriptome analysis using a bioinformatics approach to exam the functions of lncRNAs response to Ostreid herpesvirus 1μVar (OsHV-1μVar) challenge. In total, 101 differentially expressed lncRNAs (DE-lncRNA) during OsHV-1μVar infections were identified...
July 13, 2018: Fish & Shellfish Immunology
Kwanta Thansa, Ruttachuk Rungsiwiwut, Narisorn Kitiyanant, Suparat Taengchaiyaphum
To achieve in creating permanent shrimp cell lines, cellular arrest of primary cells in the culture is needed to be firstly solved. Considering the insertion of some markers affecting cellular proliferation into primary haemocytes in order to produce the black tiger shrimp cell line and the very low percent of transduced cells previously reported in penaeid shrimps, these paved us the way to set up suitable gene delivery protocols to increase percent of transduced cells in the shrimp as our primary aim. In this study, electroporation and lipofection were used to transfer construct plasmids (pLL3...
July 13, 2018: Fish & Shellfish Immunology
Cong Zhang, Yangyang Pang, Qian Zhang, Genyong Huang, Minjie Xu, Boping Tang, Yongxu Cheng, Xiaozhen Yang
In the pond culture of Eriocheir sinensis, high limb-autotomy seriously affects the quality and culture's economic efficiency. Based on our previous studies, limb autotomy can induce the changes of hematological immune response in E. sinensis hemolymph. Eyestalk ablation can accelerate the regeneration of limbs after autotomy. To detect the important functional genes related to the hematological molecular immunity of E. sinensis, we compared and analyzed the hemolymph transcriptome data of the intact crab, left cheliped autotomized crabs and bilateral eyestalk ablation crabs with high-throughput sequencing techniques...
July 12, 2018: Fish & Shellfish Immunology
Ali Taheri Mirghaed, Seyyed Morteza Hoseini, Melika Ghelichpour
The aim of the present study was to investigate beneficial effects of dietary 1,8-cineole (cineole) supplementation on physiological, immunological and antioxidant responses to crowding stress in rainbow trout (Oncorhynchus mykiss). The fish were fed for 50 days with diets containing 0 (control), 0.05, 0.1, 0.25, 0.5 and 1% cineole prior to exposure to a 14-day crowding stress. Serum stress markers (cortisol, glucose, lactate, T4 and T3 ), antioxidant responses [catalase (CAT) and superoxide dismutase (SOD) activities and total antioxidant capacity (TAC) and malondyaldehyde (MDA) levels] and immune responses [lysozyme and alternative complement (ACH50) activity and total immunoglobulin (Ig) levels], and blood leukocyte (WBC) and differential counts were measured before and after crowding stress...
July 12, 2018: Fish & Shellfish Immunology
Chao Li, Mengyu Tian, Lu Zhang, Qiang Fu, Lin Song, Fei Chen, Ning Yang
Scavenger receptors (SRs) are a group of membrane-bound receptors that could bind to a variety of ligands including endogenous proteins and pathogens. SRs have been recognized to play vital roles in innate immune response against pathogen infection in both vertebrates and invertebrates. In this regard, one SmSCARA5 gene was captured in turbot (Scophthalmus maximus). The full-length SmSCARA5 transcript contains an open reading frame (ORF) of 1494 bp. SmSCARA55 showed both the highest identity and similarity to half-smooth tongue sole (Cynoglossus semilaevis), and a high degree of conservation of genomic structure to the teleost species...
July 10, 2018: Fish & Shellfish Immunology
N N Kostin, T V Bobik, E M Shurdova, R H Ziganshin, E A Surina, D A Shagin, I A Shagina, V D Knorre, V A Isaev, G N Rudenskaya, A G Gabibov, I V Smirnov
Serpins are a family of serine protease inhibitors that are involved in numerous physiological processes and are known to regulate innate immunity pathways. To advance our understanding of their role in P. camtschaticus, a commercially significant species, we cloned and characterized a serpin from this species, designated serpin PC, that has anticoagulant and anticomplement effects on human blood. We found that serpin PC is a secreted protein with a typical serpin-like primary structure that is similar to other known crustacean serpins...
July 10, 2018: Fish & Shellfish Immunology
Yulong Zhang, Di Wang, Zao Zhang, Zhangping Wang, Daochuan Zhang, Hong Yin
To enhance genomic resources and understand the molecular immune mechanisms underlying the response topathogens, we first performed a comparative gene transcription analysis from Micrococcus lysodeikticus-immunized Artemia sinica and from a control group through RNA-Seq technology, meanwhile the differentially expressed genes (DEGs) were investigated. In total, 80, 113, 984 clean reads were obtained and then assembled into 71,536 unigenes with an average length of 1115 bp and an N50 of 1783 bp. Unigenes were annotated by comparing against nr, Swiss-Prot\KEGG\ COG\ KOG\ GO and Pfam databases, and 27,689 unigenes (38...
July 10, 2018: Fish & Shellfish Immunology
Cheng Luo, Blessing Gwekwe, Phares Choto, Wenhua Miao, Meiling Chen, Changfeng Xue, Yunsheng Xu, Xiaolong Yin, Glory Magawa, Di Wu, Jamal S Akida, Likui Wang, Qingqing Li, Shanggui Deng
The large yellow croakers (Larimichthys crocea) are mainly present in the Chinese coast and near seas with high economic importance, but vulnerable to many diseases, especially in the breeding and aquaculture. The purpose of this research was to boost the innate immune system of the large yellow croaker by administering bitter peptides into their peritoneal cavity. Total 120 Juvenile of large yellow croakers in very even weight of 60 g were divided into 4 different groups in 200/300 L of water tank, respectively...
July 10, 2018: Fish & Shellfish Immunology
Beibei Zhang, Gang Luo, Lingmin Zhao, Lixing Huang, Yingxue Qin, Yongquan Su, Qingpi Yan
Pseudomonas plecoglossicida is a threatening and important pathogen in aquaculture and causes heavy losses. Expression of L321_RS19110 was found significant up-regulated at 18 °C than at 28 °C, which was confirmed by quantitative real-time PCR. RNAi significantly reduced the content of L321_RS19110 mRNA of P. plecoglossicida, and exhibited the best efficiency of gene silencing with a reduction of 84.9%. Compared with the wild type strain, the infection of L321_RS19110-RNAi-1 strain resulted in the onset time delay, and 30% reduction in mortality of Epinephelus coioides, as well as alleviates in the symptoms of E...
July 10, 2018: Fish & Shellfish Immunology
He Wei, Mengyuan Lv, Chao Wen, Anying Zhang, Kun Yang, Hong Zhou, Xinyan Wang
Intercellular cell adhesion molecule-1 (ICAM-1) is a single-chain transmembrane glycoprotein which plays key roles in transendothelial migration of leukocytes and interaction between antigen presenting cells and T cells. In teleost, information of cell adhesion-related molecules is still lacking. In this study, we identified a gene from grass carp sharing similar exon and intron organization with human ICAM-1. Cloning and in silico analysis of its homologues in zebrafish and other two cyprinid fishes, respectively demonstrated the existence of the gene in these fishes...
July 5, 2018: Fish & Shellfish Immunology
Francesca Cima, Valerio Matozzo
Ultrastructural investigation confirmed the presence of four cell types (granulocytes, hyalinocytes, serous cells, and haemoblasts) in the haemolymph of the Manila clam, Ruditapes philippinarum. Granulocytes were characterised by numerous electron-dense granules, whereas hyalinocytes had a considerable number of small clear vesicles. Serous cells exhibited large vacuoles, which filled the cytoplasm, and haemoblasts (the undifferentiated cells) were small roundish cells characterised by a high nucleus/cytoplasm ratio...
July 5, 2018: Fish & Shellfish Immunology
Chen-Hung Ting, Yi-Chun Chen, Jyh-Yih Chen
Artemia are often used as a live feed for fry in aquaculture. We have previously demonstrated that supplementing adult zebrafish feed with Artemia, which express an Epinephelus coioides-derived antimicrobial peptide, Epinecidin-1 (Epi-1), protects against bacterial infection. Thus, Artemia may serve as a bioreactor for producing biofunctional molecules. However, the application of Epi-1 transgenic Artemia in larval aquaculture of commercial fish species has not been investigated. Here we used a Tol2-transposon system to generate stable Epi-1 expressing Artemia...
July 5, 2018: Fish & Shellfish Immunology
Wei Shi, Xiaofan Guan, Yu Han, Shanjie Zha, Jun Fang, Guoqiang Xiao, Maocang Yan, Guangxu Liu
The extensive use of TiO2 nanoparticles (nTiO2 ) in industrial products has led to their release into the marine environment, thereby posing a potential risk to marine organisms. However, in addition to affecting marine organisms through its inherent properties, nTiO2 can also act as a vehicle for other toxic pollutants due to their strong adsorption ability through the "Trojan horse" effect. Due to their potential hazard, the endocrine disrupting chemicals (EDCs) such as 17β-estradiol (E2), have been considered as one of the most serious anthropogenic threats to biodiversity and ecosystem health...
July 5, 2018: Fish & Shellfish Immunology
S Torrecillas, F Rivero-Ramírez, M S Izquierdo, M J Caballero, A Makol, P Suarez-Bregua, A Fernández-Montero, J Rotllant, D Montero
The aim of this study was to assess the effects of dietary mannan oligosaccharides (MOS), Pediococcus acidilactici or their conjunction as a synbiotic in low fish meal (FM) and fish oil (FO) based diets on European sea bass (Dicentrarchus labrax) disease resistance and gut health. For that purpose, sea bass juveniles were fed one of 6 diets containing different combinations of MOS (Biomos® and Actigen© ; Alltech, Inc., Kentucky, USA) and Pediococcus acidilactici (BAC, Bactocell® ; Lallemand Inc., Cardiff, UK) replacing standard carbohydrates as follows (MOS (%)/BAC (commercial recommendation): high prebiotic level (HP) = 0...
July 5, 2018: Fish & Shellfish Immunology
Yu-Feng Shen, Lei Liu, Chen-Zhang Feng, Yang Hu, Cheng Chen, Gao-Xue Wang, Bin Zhu
Coumarin as a lead structure have received a considerable attention in the last three decades for the discovery of antiviral agents. Our previous study indicated that imidazole coumarins possessed antiviral activities against SVCV. Based on the structure-activity relationship in that study, a new imidazole coumarin derivative, 7-(4-benzimidazole-butoxy)-coumarin (BBC), was designed, synthesized and its anti-SVCV activity was evaluated. By comparing inhibitory concentration at half-maximal activity (IC50 ), we found that BBC (IC50  = 0...
July 4, 2018: Fish & Shellfish Immunology
Rahul Krishnan, K Jeena, Kurcheti Pani Prasad
Member of the dynamin family of large GTPases, dynamin-related protein 1 (Drp1) dependent mitochondrial fission is an intricate process regulating both cellular and organ dynamics. Present study shows that NNV perturbs mitochondrial dynamics by promoting Drp-1 dependent mitochondrial fission, which attenuates MAVS mediated downstream signaling. NNV infected SISS cells revealed induction in Drp1 expression and subsequent translocation into mitochondria. The level of MAVS expression was up-regulated over a period of 24 hpi and declined with the progression of NNV infection at 48 and 72 hpi confirmed by western blot and mRNA transcript analysis...
July 4, 2018: Fish & Shellfish Immunology
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