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Fish & Shellfish Immunology

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https://www.readbyqxmd.com/read/28532667/evaluation-of-three-recombinant-outer-membrane-proteins-ompa1-tdr-and-tbpa-as-potential-vaccine-antigens-against-virulent-aeromonas-hydrophila-infection-in-channel-catfish-ictalurus-punctatus
#1
Hossam Abdelhamed, Iman Ibrahim, Seong Won Nho, Michelle M Banes, Robert W Wills, Attila Karsi, Mark L Lawrence
A virulent clonal population of Aeromonas hydrophila (VAh) is recognized as the etiological agent in outbreaks of motile aeromonas septicemia (MAS) in catfish aquaculture in the southeastern United States since 2009. Genomic subtraction revealed three outer membrane proteins present in VAh strain ML09-119 but not in low virulence reference A. hydrophila strains: major outer membrane protein OmpA1, TonB-dependent receptor (Tdr), and transferrin-binding protein A (TbpA). Here, the genes encoding ompA1, tdr, and tbpA were cloned from A...
May 19, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28532666/transcription-of-immune-related-genes-in-solea-senegalensis-vaccinated-against-photobacterium-damselae-subsp-piscicida-identification-of-surrogates-of-protection
#2
J A Núñez-Díaz, I García de la Banda, C Lobo, M A Moriñigo, M C Balebona
Solea senegalensis is a flatfish with a great potential for aquaculture, but infectious diseases restrict its production, being this fish species highly susceptible to Photobacterium damselae subsp. piscicida (Phdp) infections. A better understanding of the mechanisms related to fish immune response is crucial for the development of effective approaches in disease management. In the present work, transcriptional changes of immune related genes have been evaluated in farmed S. senegalensis specimens vaccinated against Phdp by intraperitoneal injection (IP) and immersion (IM)...
May 19, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28528707/molecular-transcriptional-and-functional-insights-into-duplicated-goose-type-lysozymes-from-sebastes-schlegelii-and-their-potential-immunological-role
#3
Jehanathan Nilojan, S D N K Bathige, Roopasingam Kugapreethan, W S Thulasitha, Bo-Hye Nam, Jehee Lee
Black rockfish (Sebastes schlegelii), an important aquaculture species in Korea, has been affected by bacterial diseases leading to a drastic decline in production. Goose-type lysozyme (LysG) is a key enzyme of the innate immune system to eradicate bacterial infections. In this study, two isoforms of LysG from black rockfish, designated as RfLysG1 and RfLysG2, have been identified and characterized at the molecular, transcriptional, and functional levels. The deduced amino acid sequences had the LysG family characteristics and exhibited conserved properties, including active residues and domains...
May 18, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28527896/molecular-characterization-and-expression-analyses-of-the-solea-senegalensis-interferon-stimulated-gene-15-isg15-following-nnv-infections
#4
Daniel Álvarez-Torres, Ana María Podadera, M Carmen Alonso, Isabel Bandín, Julia Béjar, Esther García-Rosado
Interferons are essential in fish resistance to viral infections. They induce interferon-stimulated genes, such as isg15. In this study, the Senegalese sole isg15 gene (ssisg15) has been characterized. As other isg15, ssisg15 contains a 402-bp intron sited in the 5'-UTR, and the full length cDNA is 1492-bp, including a 480-bp ORF. The expression analyses revealed basal levels of isg15 transcripts, and a clear induction after poly I:C injection, that reached maximum values in brain, head kidney and gills. The ssisg15 induction patterns were similar in RGNNV- and SJNNV-inoculated fish, whereas the reassortant (RG/SJ) isolate, which has higher replication fitness, triggered delayed but higher transcript levels...
May 17, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28527895/suppression-of-pmrab11-inhibits-yhv-infection-in-penaeus-monodon
#5
Akechai Kongprajug, Sakol Panyim, Chalermporn Ongvarrasopone
Yellow head virus (YHV) is one of the most serious pathogens that causes worldwide shrimp production loss. It enters the cells via clathrin-mediated endocytosis and utilizes small GTPase Rab proteins such as PmRab5 and PmRab7 for intracellular trafficking. In this study, molecular cloning and functional analysis of Rab11 during YHV infection were investigated. PmRab11 cDNA was cloned by Rapid amplification of cDNA ends (RACEs). It contained two forms of sizes 1200 and 1050 bp distinct at the 5' UTR. The coding region of PmRab11 was 645 bp, encoding 214 amino acids...
May 17, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28526573/transcriptomic-variation-of-locally-infected-skin-of-epinephelus-coioides-reveals-the-mucosal-immune-mechanism-against-cryptocaryon-irritans
#6
Yazhou Hu, Anxing Li, Yang Xu, Biao Jiang, Geling Lu, Xiaochun Luo
Fish skin is the largest immunologically active mucosal organ, providing first-line defense against external pathogens. However, the skin-associated immune mechanisms of fish are still unclear. Cryptocaryon irritans is an obligate ectoparasitic ciliated protozoan that infects almost all marine fish, and is believed to be an excellent pathogen model to study fish mucosal immunity. In this study, a de novo transcriptome assembly of Epinephelus coioides skin post C. irritans tail-infection was performed for the first time using the Illumina HiSeq™ 2500 system...
May 16, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28526572/comprehensive-evaluation-of-immunomodulation-by-moderate-hypoxia-in-s-agalactiae-vaccinated-nile-tilapia
#7
Sanchala Gallage, Takayuki Katagiri, Masato Endo, Masashi Maita
Streptococcus agalactiae is a major bacterial pathogen in tilapia aquaculture. Vaccines are known to provide protection but S. agalactiae clearance in tilapia can be reduced by marginal environmental conditions. Therefore, the purpose of this study is to examine S. agalactiae clearance in vaccinated Nile tilapia under moderate hypoxic (55± 5% DO) and normoxic (85 ± 5%DO) conditions. Fish were acclimatized to either moderate hypoxia or normoxia and immunized with formalin-inactivated S. agalactiae. Fish were experimentally challenged with S...
May 16, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28526571/the-antiviral-signaling-mediated-by-black-carp-mda5-is-positively-regulated-by-lgp2
#8
Ji Liu, Jun Li, Jun Xiao, Hui Chen, Liang Lu, Xu Wang, Yu Tian, Hao Feng
Melanoma differentiation-associated gene 5 (MDA5) belongs to RIG-I like receptor (RLR) family, which detects cytosolic viral RNA component in immune response. In this study, MDA5 orthologue of black carp (Mylopharyngodon piceus) has been cloned and characterized. The full-length cDNA of black carp MDA5 (bcMDA5) comprises 3244 nucleotides and the predicted bcMDA5 protein contains 984 amino acids. The constitutive transcription of bcMDA5 was extremely low in all the tested tissues, which included gill, skin, muscle, intestine, kidney, spleen, liver and heart...
May 16, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28526570/role-of-medicinal-plants-on-growth-performance-and-immune-status-in-fish
#9
REVIEW
Elham Awad, Amani Awaad
Disease outbreaks increase proportionally with increases in intensive aquaculture. Natural products including medicinal plants have been known from thousands of years for treating some human diseases. It is well known that many active compounds are responsible for potential bio-activities. For that reason, there has been considerable interest in the use of medicinal plants in aquaculture with a view to providing safe and eco-friendly compounds for replacing antibiotics and chemical compounds as well as to enhance immune status and control fish diseases...
May 16, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28522421/a-chymotrypsin-like-serine-protease-from-portunus-trituberculatus-involved-in-pathogen-recognition-and-amp-synthesis-but-not-required-for-prophenoloxidase-activation
#10
Hourong Liu, Yuan Liu, Chengwen Song, Zhaoxia Cui
Clip domain serine proteases (clip-SPs) play critical roles in various immune responses in arthropods, such as hemolymph coagulation, antimicrobial peptide (AMP) synthesis, cell adhesion and melanization. In the present study, we report the molecular and functional characterization of a clip domain serine protease (PtcSP2) from the swimming crab Portunus trituberculatus. The N-terminal clip domain and the C-terminal SP-like domain of PtcSP2 were expressed in Escherichia coli system, and assayed for their activities...
May 15, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28522420/in%C3%A2-vitro-effects-of-italian-lavandula-multifida-l-leaf-extracts-on-gilthead-seabream-sparus-aurata-leucocytes-and-saf-1-cells
#11
Angela Fazio, Rebeca Cerezuela, Maria Rosaria Panuccio, Alberto Cuesta, Maria Ángeles Esteban
Lavandula multifida is very appreciated by pharmaceutical and cosmetic industries. In Italy is only found in Calabria and Sicily and, at present, urge its valorization due to its high extinction and genetic erosion risks. Possible applications of L. multifida extracts as immunostimulant in fish aquaculture were assayed by using gilthead seabream (Sparus aurata) as a marine fish model, due to its importance in fish aquaculture. The in vitro effects of both aqueous and ethanolic leaf extracts obtained from two Italian populations of L...
May 15, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28522419/effects-of-stocking-density-of-the-white-shrimp-litopenaeus-vannamei-boone-on-immunities-antioxidant-status-and-resistance-against-vibrio-harveyi-in-a-biofloc-system
#12
Gang Liu, Songming Zhu, Dezhao Liu, Xishan Guo, Zhangying Ye
Determining optimum stocking density of the white shrimp Litopenaeus vannamei (Boone) is a big concern for shrimp farmers. However, few studies have assessed the influence of stocking density on the antioxidant status, immunology, digestive enzyme activities, and growth performance of white shrimp in biofloc systems. In this study, these parameters of white shrimp in a biofloc system were compared at three stocking densities: 300 orgs m(-3) as low stocking density (LD), 400 orgs m(-3) as medium stocking density (MD), and 500 orgs m(-3) as high stocking density (HD)...
May 15, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28522418/immunotoxicity-of-nanoparticle-ntio2-to-a-commercial-marine-bivalve-species-tegillarca-granosa
#13
Wei Shi, Yu Han, Cheng Guo, Xinguo Zhao, Saixi Liu, Wenhao Su, Shanjie Zha, Yichen Wang, Guangxu Liu
The increasing production and extensive application of nanoparticles (NPs) inevitably leads to increased release of NPs into the marine environment and therefore poses a potential threat to marine organisms, especially the sessile benthic bivalves. However, the impacts of NPs on the immunity of commercial and ecological important bivalve species, Tegillarca granosa, still remain unknown to date. In addition, the molecular mechanism of the immunotoxicity of NPs still remains unclear in marine invertebrates. Therefore, the immunotoxicity of nTiO2 exposure to T...
May 15, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28511951/proteome-profiling-reveals-immune-responses-in-japanese-flounder-paralichthys-olivaceus-infected-with-edwardsiella-tarda-by-itraq-analysis
#14
Lei Wang, Changwei Shao, Wenteng Xu, Qian Zhou, Na Wang, Songlin Chen
The liver is an important organ for bacterial pathogen attack in fish. The differential proteomic response of the Japanese flounder liver to Edwardsiella tarda infection was examined using isobaric tags for relative and absolute quantitation (iTRAQ) labeling followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). A total of 3290 proteins were identified and classified into categories related to biological process (51.4%), molecular function (63.6%), and cellular component (57.7%). KEGG enrichment analysis indicated the complement and coagulation cascade pathways and the mineral absorption pathway were significantly enriched...
May 13, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28501446/the-involvement-of-cathepsin-f-gene-ctsf-in-turbot-scophthalmus-maximus-l-mucosal-immunity
#15
Chengbin Gao, Qiang Fu, Baofeng Su, Huanhuan Song, Shun Zhou, Fenghua Tan, Chao Li
Cathepsin F (CTSF) is a recently described papain-like cysteine protease and unique among cathepsins due to an elongated N-terminal pro-region, which contains a cystatin domain. CTSF likely plays a regulatory role in processing the invariant chain which is associated with the major histocompatibility complex (MHC) class II. In this regard, we identified the CTSF gene of turbot as well as its protein structure, phylogenetic relationships, and expression patterns in mucosal tissues following Vibrio anguillarum and Streptococcus iniae challenge...
May 10, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28501445/local-and-systemic-humoral-immune-response-in-farmed-atlantic-salmon-salmo-salar-l-under-a-natural-amoebic-gill-disease-outbreak
#16
Mar Marcos-López, Cristóbal Espinosa Ruiz, Hamish D Rodger, Ian O'Connor, Eugene MacCarthy, M Ángeles Esteban
Amoebic gill disease (AGD), caused by the protozoan parasite Neoparamoeba perurans, is one of the most significant infectious diseases for Atlantic salmon (Salmo salar L.) mariculture. The present study investigated the humoral immune response (both local in gill mucus and systemic in serum) of farmed Atlantic salmon naturally infected with N. perurans in commercial sea pens, at two different stages of the disease and after freshwater treatment. Parameters analysed included activity of immune related enzymes (i...
May 10, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28499967/molecular-cloning-expression-and-antioxidative-activity-of-2-cys-peroxiredoxin-from-freshwater-mussel-cristaria-plicata
#17
Xiaobo Wang, Baoqing Hu, Chungen Wen, Ming Zhang, Shaoqing Jian, Gang Yang
Peroxiredoxins (Prxs) play an important role against various oxidative stresses by catalyzing the reduction of hydrogen peroxide (H2O2) and organic hydroperoxides to less harmful form. A 2-cys peroxiredoxin, designated as CpPrx, was cloned from hemocytes of freshwater mussel Cristaria plicata. The full length cDNA of CpPrx is 1247 bp, which includes an open reading frame (ORF) of 591bp, encoding 196 amino acids. CpPrx possesses two conserved cysteine residues (Cys49, Cys170). The deduced amino acid sequence of CpPrx showed a high level (67-74%) of sequence similarity to 2-Cys Prxs from other species...
May 9, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28499966/interferon-induced-protein-56-ifi56-is-induced-by-vhsv-infection-but-not-by-bacterial-infection-in-olive-flounder-paralichthys-olivaceus
#18
Jee Youn Hwang, Sang Jung Ahn, Mun-Gyeong Kwon, Jung Soo Seo, Seong Don Hwang, Maeng-Hyun Son
Interferon-inducible protein 56 (IFI56, also known as ISG56/IFIT1, interferon-induced protein with tetratricopeptide repeats 1) is strongly induced in response to interferon and a potent inhibitor of viral replication and translational initiation. Here, we describe the identification of IFI56 (OfIFI56) in olive flounder, its characteristic features, and expression levels in various tissues before and after viral hemorrhagic septicemia virus (VHSV) infection. The full-length OfIFI56 sequence was identified from rapid amplification of cDNA ends PCR...
May 9, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28499965/effects-of-dietary-dandelion-extracts-on-growth-performance-body-composition-plasma-biochemical-parameters-immune-responses-and-disease-resistance-of-juvenile-golden-pompano-trachinotus-ovatus
#19
Xiaohong Tan, Zhenzhu Sun, Shu Chen, Silin Chen, Zhong Huang, Chuanpeng Zhou, Cuiyun Zou, Qingying Liu, Huaqun Ye, Heizhao Lin, Chaoxia Ye, Anli Wang
The present study was conducted to investigate the effects of dietary dandelion extracts (DE) supplementation on growth performance, feed utilization, body composition, plasma biochemical indices, immune responses, hepatic antioxidant abilities, and resistance to the pathogen Vibrio harveyi in Trachinotus ovatus. A basal diet supplemented with DE at 0, 0.50, 1.00, 2.00, 4.00 and 10.00 g kg(-1) were fed to golden pompano for 8 weeks. The study indicated that dietary supplementation with DE could significantly improve final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR), feed efficiency ratio (FER), feed intake (FI), protein efficiency ratio (PER) and protein deposit rate (PDR) (P < 0...
May 9, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28495510/in%C3%A2-vitro-characterization-of-grass-carp-ctenopharyngodon-idella-il-26-in-regulating-inflammatory-factors
#20
Xingyang Qiu, Mengyuan Lv, Xiaoyu Jian, Di Chen, Hong Zhou, Anying Zhang, Xinyan Wang
Interleukin 26 (IL-26) gene has been identified in human, amphibian and teleost but not in rodents. It is well accepted that IL-26 was a crucial member of IL-10 family which acts as a pro-inflammatory cytokine in human. However, the role of IL-26 in regulating inflammation in lower vertebrates including teleost has not been defined yet. In the present study, grass carp IL-26 (gcIL-26) coding sequence was isolated and identified. Its chromosomal synteny was also analyzed, showing that gcIL-26 gene is flanked by IL-22 and IFN-γ genes with the same transcriptional orientation as seen in human, amphibian and zebrafish...
May 8, 2017: Fish & Shellfish Immunology
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