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Fish & Shellfish Immunology

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https://www.readbyqxmd.com/read/28442417/cloning-expression-analysis-and-antibacterial-propertiesof-three-serum-amyloid-a-in-common-carp-cyprinus-carpio
#1
Juhua Yu, Yongkai Tang, Jianlin Li, Hongxia Li, Fan Yu, Wenjuan Yu, Feng He, Chunjie Fu, Shuntao Mao
Three serum amyloid A (SAA) genes were identified from the common carp (Cyprinus carpio) by PCR and RT-PCR. Considering both direction and sequence similarity with mammal's orthologs, they were named CcSAA3a, CcSAA3b and CcSAA1. CcSAA3b and CcSAA1 are adjacent on contig LHQP01017858, suggesting that the prototype of or the simplest SAA multigene family have occurred in common carp. A phylogenetic analysis of the SAAs indicated that the fish SAAs were closer to those of invertebrates and Ornithorhynchus anatinus, a primitive mammal, than to mammalian SAAs...
April 23, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28442419/a-sulfated-galactans-supplemented-diet-enhances-the-expression-of-immune-genes-and-protects-against-vibrio-parahaemolyticus-infection-in-shrimp
#2
Tawut Rudtanatip, Nantavadee Boonsri, Somluk Asuvapongpatana, Boonsirm Withyachamnarnkul, Kanokpan Wongprasert
A sulfated galactans (SG) supplemented diet was evaluated for the potential to stimulate immune activity in shrimp Penaeus vannamei (P. vannamei). Shrimp given the SG supplemented diet (0.5, 1 and 2% w/w) for 7 days showed enhanced expression of the downstream signaling mediator of lipopolysaccharide and β-1,3-glucan binding protein (LGBP) and immune related genes including p-NF-κB, IMD, IKKβ and IKKε, antimicrobial peptide PEN-4, proPO-I and II. Following immersion with Vibrio parahaemolyticus (V. parahaemolyticus) for 14 days, the shrimp given the SG supplemented diet (1 and 2% w/w) showed a decrease in bacterial colonies and bacterial toxin gene expression, compared to shrimp given a normal diet, and they reached 50% mortality at day 14...
April 22, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28442418/tlr22-structure-and-expression-characteristic-of-barbel-chub-squaliobarbus-curriculus-provides-insights-into-antiviral-immunity-against-infection-with-grass-carp-reovirus
#3
Rong-Hua Wang, Wei Li, Yu-Ding Fan, Qiao-Lin Liu, Ling-Bing Zeng, Tiao-Yi Xiao
Grass carp reovirus (GCRV) is the most virulent agent to Grass carp, Ctenopharyngodon idella, and causes a severe infectious disease called hemorrhagic disease of grass carp. Generally, barbel chub, Squaliobarbus curriculus, a genetically closely related species to grass carp, exhibits significant resistance against GCRV infection compared to grass carp. To investigate whether the Toll-like receptor 22 (tlr22) has got a vital role against the GCRV infection, the full cDNA sequence of tlr22 from barbel chub (Sctlr22) was cloned by RACE-PCR, and the structure and expression feature were studied...
April 22, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28442416/molecular-characterization-and-expression-of-interleukin-10-and-interleukin-22-in-golden-pompano-trachinotus-ovatus-in-response-to-streptococcus-agalactiae-stimulus
#4
Yinhui Peng, Xiaohui Cai, Guoyin Zhang, Junlin Wang, Yuan Li, Zhiwen Wang, Bei Wang, Xiangying Xiong, Zaohe Wu, Jichang Jian
In the present study, members of the interleukin (IL)-10 family of cytokines, including IL-10 (TOIL-10) and IL-22 (TOIL-22) of golden pompano (Trachinotus ovatus), were cloned for the first time, and their expression patterns and 3D structures analyzed. The full-length cDNA sequences of TOIL-10 and TOIL-22 contained open reading frames of 564 and 567 bp, respectively. TOIL-10 and TOIL-22 shared higher homology (78%-89%) with the corresponding genes from various fish relative to other species (25%-34%) and contained the IL-10 family signature and four cysteine residues that are well conserved in other vertebrate IL-10 members...
April 22, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28433720/establishment-of-a-non-invasive-method-for-stress-evaluation-in-farmed-salmon-based-on-direct-fecal-corticoid-metabolites-measurement
#5
Yanran Cao, Ann-Kristin Tveten, Anne Stene
Fish welfare is an important issue for growth of the aquaculture industry. Stress responses represent animal's natural reactions to challenging conditions and could be used as a welfare indicator. Cortisol level is relevant to fish welfare condition, and is a readily measured component of the primary stress response system. Generally, cortisol is measured by blood sampling. However, fish blood cortisol level could be instantly influenced by handling-stress at sampling. Fecal corticoid metabolites (FCM) are a mixture of several different metabolites with a wide range of polarities...
April 19, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28433719/isolation-and-characterization-of-monocyte-macrophage-from-peripheral-blood-of-half-smooth-tongue-sole-cynoglossus-semilaevis
#6
Zhenxia Sha, Linqing Wang, Luming Sun, Yadong Chen, Yuan Zheng, Mei Xin, Chen Li, Songlin Chen
In the present study, the peripheral blood cells of half smooth tongue sole (Cynoglossus semilaevis) were examined by blood smear under the light microscopy. The proportion of main types of blood cells are as following: erythrocyte occupied the majority (92.3%), followed by thrombocyte (4.15%), granulocyte (1.7%), lymphocyte (1.5%) and monocyte (0.3%), respectively. Meanwhile, the isolation method of monocytes was established, by density gradient centrifugation to isolate mononuclear leukocytes of peripheral blood...
April 19, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28433718/three-new-piscidins-from-orange-spotted-grouper-epinephelus-coioides-phylogeny-expression-and-functional-characterization
#7
Ze-Rui Zhuang, Xiao-Dong Yang, Xia-Zi Huang, Hui-Xian Gu, Han-Yin Wei, Yi-Jun He, Li Deng
The present study reports the identification, and characterization of three new putative piscidin paralogues, ecPis-2, ecPis-3 and ecPis-4, from orange-spotted grouper (Epinephelus coioides). The cDNA of the three piscidins with the 207, 216, and 231 nt open reading frame encoded respectively a 68-, 71-, and 76-amino acid preprotein consisting of the predicted signal peptide, and putative mature peptide and prodomain. The phylogenetic analysis indicated that multiple piscidin paralogues in one fish species are highly diversified, the analysis suggested that the piscidins should be a family belonging to the superfamily of ancient cationic, linear, and amphipathic host defence peptides widespread across invertebrate and vertebrate taxa comprising insect cecropins and ceratotoxins, and the amphibian dermaseptins...
April 19, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28433717/response-a-chronic-effects-of-pbde-47-up-regulations-of-hsp60-and-hsp70-expression-in-freshwater-bivalve-anodonta-woodiana
#8
Xichao Xia, Shipeng Xue, Xiying Wang, Qingyuan Zhang, Chuanfeng Huang, Lianghong Guo, Lunguang Yao
Heat shock proteins (HSPs) play an important role in adaption of environmental stress by protein folding, membrane translocation, degradation of misfolded proteins and other regulatory processes. Our previous study showed oxidative stress generated from polybrominated diphenyl ether-47 (PBDE-47) could cause an acute toxicity on freshwater bivalve Anodonta Woodiana, but the effect of chronic toxicity need to be elucidated. In order to further investigate the chronic effect of PBDE-47, clams A. Woodiana were randomly divided into the PBDE-47 treated group administrated with PBDE-47 at a concentration 3...
April 19, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28433716/development-of-a-monoclonal-antibody-against-the-cd3%C3%AE%C2%B5-of-olive-flounder-paralichthys-olivaceus-and-its-application-in-evaluating-immune-response-related-to-cd3%C3%AE%C2%B5
#9
Jae Wook Jung, Jung Seok Lee, Young Rim Kim, Se Pyeong Im, Si Won Kim, Jassy Mary S Lazarte, Jaesung Kim, Kim D Thompson, Jong Pyo Suh, Tae Sung Jung
The T cell receptor (TCR) is the binding site of antigen and is responsible for specifically activating the adaptive immune response. CD3, an essential component of the CD3-TCR complex, is known to be composed of γδ and ε chains in teleost. However, there are few monoclonal antibodies (mAb) available to identify these molecules on T cells, so we aimed to produce a mAb against CD3ε to improve our understanding of T cell immune response in olive flounder (Paralichthys olivaceus). CD3ε recombinant protein was expressed in yeast, the expression of which was confirmed by SDS-PAGE, MALDI-TOF/TOF MS and Western blot analysis...
April 19, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28433715/aloe-vera-enhances-the-innate-immune-response-of-pacu-piaractus-mesopotamicus-after-transport-stress-and-combined-heat-killed-aeromonas-hydrophila-infection
#10
Fábio S Zanuzzo, Rafael E Sabioni, Luz Natalia F Montoya, Gisele Favero, Elisabeth C Urbinati
In this study, pacu (Piaractus mesopotamicus) were fed with diets containing Aloe vera for 10 days prior to transport stress and infection with heat killed Aeromonas hydrophila. A. vera is popular around the world due to its medicinal properties, including immunostimulatory effects which was observed in this study. The results show that transport causes immunosuppression, an effect that was prevented by A. vera. Specifically, A. vera prevented reductions of both leukocyte respiratory burst and hemolytic activity of complement system caused by transport...
April 19, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28433714/effects-of-dietary-supplementation-with-fenugreek-seeds-alone-or-in-combination-with-probiotics-on-gilthead-seabream-sparus-aurata-l-skin-mucosal-immunity
#11
F A Guardiola, A Bahi, A Bakhrouf, M A Esteban
Despite increasing interest in modulating the immune response of fish, providing a combination of probiotics and herbal immunostimulants in aquafeed has rarely has been studied. The effects on gilthead seabream (Sparus aurata L.) of the dietary administration of fenugreek (Trigonella foenum graecum) seeds alone (FE), or combined with one of the following probiotic strains: Bacillus licheniformis (FEBL), Lactobacillus plantarum (FELP) or Bacillus subtilis (FEBS) were evaluated. Fish were fed a control or one of the supplemented diets for 3 weeks...
April 19, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28428059/the-decreased-growth-performance-and-impaired-immune-function-and-structural-integrity-by-dietary-iron-deficiency-or-excess-are-associated-with-tor-nf-%C3%AE%C2%BAb-p38mapk-nrf2-and-mlck-signaling-in-head-kidney-spleen-and-skin-of-grass-carp-ctenopharyngodon-idella
#12
Yan-Lin Guo, Wei-Dan Jiang, Pei Wu, Yang Liu, Xiao-Qiu Zhou, Sheng-Yao Kuang, Ling Tang, Wu-Neng Tang, Yong-An Zhang, Lin Feng
This study was conducted to investigate the effects of dietary iron on the growth, and immune function and structural integrity in head kidney, spleen and skin as well as the underlying signaling of young grass carp (Ctenopharyngodon idella). Total 630 grass carp (242.32 ± 0.58 g) were fed diets containing graded levels of iron at 12.15 (basal diet), 35.38, 63.47, 86.43, 111.09, 136.37 mg/kg (diets 2-6 were added with ferrous fumarate) and 73.50 mg/kg (diet 7 was added with ferrous sulfate) diet for 60 days...
April 18, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28428060/effects-of-chronic-ammonia-exposure-on-ammonia-metabolism-and-excretion-in-marine-medaka-oryzias-melastigma
#13
Na Gao, Limei Zhu, Zhiqiang Guo, Meisheng Yi, Li Zhang
Ammonia is highly toxic to aquatic organisms, but whether ammonia excretion or ammonia metabolism to less toxic compounds is the major strategy for detoxification in marine fish against chronic ammonia exposure is unclear to date. In this study, we investigated the metabolism and excretion of ammonia in marine medaka Oryzias melastigma during chronic ammonia exposure. The fish were exposed to 0, 0.1, 0.3, 0.6, and 1.1 mmol l(-1) NH4Cl spiked seawater for 8 weeks. Exposure of 0.3-1.1 mmol l(-1) NH4Cl had deleterious effects on the fish, including significant reductions in growth, feed intake, and total protein content...
April 17, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28419854/a-formalin-inactivated-vaccine-provides-good-protection-against-vibrio-harveyi-infection-in-orange-spotted-grouper-epinephelus-coioides
#14
Hai Trong Nguyen, Thuy Thi Thu Nguyen, Ming-An Tsai, E Ya-Zhen, Pei-Chyi Wang, Shih-Chu Chen
Vibrio harveyi is one of the most common threats to farmed grouper, so considerable efforts are in practice to control the pathogen. This study presents a highly effective vaccine against V. harveyi in the orange-spotted grouper with the help of a single intraperitoneal immunization. The vaccine candidate was in form of whole, formalin-inactivated V. harveyi cells combined with a metabolizable ISA763 AVG adjuvant. Our results indicated that the vaccine triggered a remarkably higher expression level of interleukin (IL)-1β, IL-6, IL-8, and IL-10 in the groupers' kidneys and spleens, as recorded after 1 and 3 days of immunization...
April 15, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28416423/dietary-administration-of-sodium-alginate-ameliorated-stress-and-promoted-immune-resistance-of-grouper-epinephelus-coioides-under-cold-stress
#15
Pai-Po Lee, Yu-Hung Lin, Min-Chieh Chen, Winton Cheng
Grouper, Epinephelus coioides, fed a diet containing sodium alginate at 0 (control, named C) and 1.0 g kg(-1) (named S) at a temperature of 28 °C for 12 days, were then further individually transferred to 28 (two groups named C-28 and S-28) or 20 °C (two groups named C-20 and S-20), and immune parameters and stress indexes were measured at the beginning and after 6, 12, 24 and 48 h of exposure. Examination of immune parameters revealed that the alternative complement activity (ACH50), lysozyme activity, phagocytic activity, superoxide dismutase, and respiratory bursts significantly increased in groupers fed the sodium alginate-containing diet for 12 days, and were higher in the S-28 than those of the C-28 and S-20 groups, which were higher than those of the C-20 group from 6 to 48 h except for ACH50 at 48 h, respiratory bursts at 48 h, and lysozymes at 6 h...
April 14, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28416091/corrigendum-to-glucose-enhances-tilapia-against-edwardsiella-tarda-infection-through-metabolome-reprogramming-fish-and-shellfish-immunol-61-2017-34-43
#16
Zao-Hai Zeng, Chao-Chao Du, Shi-Rao Liu, Hui Li, Xuan-Xian Peng, Bo Peng
No abstract text is available yet for this article.
April 13, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28408239/protective-responses-of-two-paralogs-of-granulocyte-colony-stimulating-factor-gcsf-in-rock-bream-oplegnathus-fasciatus-during-bacterial-and-viral-infection
#17
Joseph Jeswin, Ji-Min Jeong, Jae-Dong Shim, Jin-Sol Bae, Chan-Il Park
Granulocyte colony stimulating factor (GCSF) has a key role in the production of neutrophilic granulocytes during normal hematopoietic development and release of neutrophils into the blood circulation. In this study we have identified and characterized two paralogs of GCSF (RbGCSF) in rock bream. Although RbGCSF-1 and RbGCSF-2 share low sequence conservation, its domains and protein structure still share significant similarity. Basal levels of RbGCSF-1 gene expression was high in peripheral blood leukocytes (PBLs), spleen and intestine whereas the RbGCSF-2 was highly expressed in PBLs and kidney, of healthy animals...
April 10, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28400215/levamisole-enhances-the-innate-immune-response-and-prevents-increased-cortisol-levels-in-stressed-pacu-piaractus-mesopotamicus
#18
Eduardo Pahor-Filho, Adriana Soliris Corredor Castillo, Nycolas Levy Pereira, Fabiana Pilarski, Elisabeth Criscuolo Urbinati
We analyzed the effects of levamisole on stress and the innate immune responses of pacu (Piaractus mesopotamicus). A total of 300 fish (180 ± 1.27 g) were fed a diet containing levamisole hydrochloride (LHC) for 15 days, then distributed into the following groups: T0 (control group); T1 (100), T2 (150), T3 (300) and T4 (500) mg kg(-1) LHC (15 fish per group and four replicates per treatment). After this, fish (n = 8 per treatment) were exposed to air for three minutes to simulate stress conditions and were then challenged with the bacterium Aeromonas hydrophila to stimulate the immune system...
April 8, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28400214/characterization-of-pmsp%C3%B3-tzle-1-from-the-black-tiger-shrimp-peneaus-monodon
#19
Sittichai Boonrawd, Ravi Mani, Sirikwan Ponprateep, Premruethai Supungul, Promsin Masrinoul, Anchalee Tassanakajon, Vichien Rimphanitchayakit
Spätzle is a signaling ligand in innate immune response that signals pathogenic infection via Toll receptor and Toll pathway into the cells for the synthesis of antimicrobial proteins. Herein, three PmSpӓtzle isoforms were identified in Penaeus monodon, namely PmSpz1, 2 and 3. The PmSpz1 was chosen for detailed study. The PmSpz1 gene was expressed in all nine tissues tested including the hemocytes, stomach, hepatopancreas, gill, lymphoid tissue, eyestalk, muscle, intestine and heart. Its expression was up-regulated upon white spot syndrome virus (WSSV) infection...
April 8, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28400213/investigation-of-8-ohdg-cyp1a-hsp70-and-transcriptional-analyses-of-antioxidant-defence-system-in-liver-tissues-of-rainbow-trout-exposed-to-eprinomectin
#20
Gonca Alak, Aslı Çilingir Yeltekin, Ismail Hakkı Tas, Arzu Ucar, Veysel Parlak, Ahmet Topal, Esat Mahmut Kocaman, Muhammed Atamanalp
Eprinomectin (EPM), a member of avermectin family, is a semi-synthetic antibiotic. It has been known that avermectin family enters the aquatic environments and adversely affects the aquatic organisms. Effects of EPM is fully unknown in aquatic organisms especially fish, thus the aim of the present study was to investigate transcriptional changes (sod, cat, gpx) and activities of some antioxidant enzymes (superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) and malondialdehyde (MDA) levels, oxidative DNA damage (8-hydroxy-2-deoxyguanosine (8-OHdG)) and transcriptional changes of heat shock protein 70 (HSP70), and cytochromes P4501A (CYP1A) in liver tissues of rainbow trout exposed to sublethal EPM concentration (0...
April 8, 2017: Fish & Shellfish Immunology
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