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Fish & Shellfish Immunology

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https://www.readbyqxmd.com/read/30654028/functional-characterization-of-a-mannose-binding-lectin-mbl-from-nile-tilapia-oreochromis-niloticus-in-non-specific-cell-immunity-and-apoptosis-in-monocytes-macrophages
#1
Liangliang Mu, Xiaoxue Yin, Yanjian Yang, Liting Wu, Hairong Wu, Bingxi Li, Zheng Guo, Jianmin Ye
Mannose-binding lectin (MBL), a soluble pattern recognition receptor, is able to recognize antigen and participate in non-specific cell immunity, such as regulation of inflammation, migration, opsonization, phagocytosis and killing, which plays an important role in innate immunity. In this study, we have investigated the contributing mechanisms and effects of MBL on the cell immunity of Nile tilapia (Oreochromis niloticus) monocytes/macrophages. The mRNA expression level of OnMBL was significantly up-regulated in monocytes/macrophages after in vitro bacterial infection (Streptococcus agalactiae and Aeromonas hydrophila)...
January 14, 2019: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30648626/identification-and-expression-of-the-hepcidin-gene-from-brown-trout-salmo-trutta-and-functional-analysis-of-its-synthetic-peptide
#2
Tianqing Huang, Wei Gu, Bingqian Wang, Yuyong Zhang, Lili Cui, Zuochun Yao, Cheng Zhao, Gefeng Xu
Hepcidin, a hepatic antimicrobial peptide, is a key player of the nonspecific immune system. The structure of hepcidin gene from brown trout (Bthepc) has been characterized at the molecular level. The 1158-bp mRNA generates a coding sequence (CDS) of 267 bp, which encodes an 88-amino acid protein. Molecular evolution analysis classified Bthepc to the family Salmonidae. Amino acid sequence homologies between Bthepc and hepcidin in other species such as Oncorhynchus mykiss, Salmo salar, and Hucho taimen were found to be 93...
January 12, 2019: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30648625/b-cell-receptor-accessory-molecule-cd79-gets-involved-in-response-against-streptococcus-agalactiae-infection-and-bcr-signaling-in-nile-tilapia-oreochromis-niloticus
#3
Liting Wu, Xia Bian, Linghe Kong, Xiaoxue Yin, Liangliang Mu, Siwei Wu, Along Gao, Xiufang Wei, Zheng Guo, Jianmin Ye
CD79, composed of two distinct chains called CD79a and CD79b, is a transmembrane protein that forms a B cell antigen receptor with membrane immunoglobulin, and generates a signal following antigen recognition by the B cell receptor. In this study, the CD79a (OnCD79a) and CD79b (OnCD79b) were cloned and identified from Nile tilapia (Oreochromis niloticus). The cDNA of ORF for OnCD79a and OnCD79b are 669 and 627 bp, coding 222 and 208 amino acids, respectively. The deduced protein analysis showed that both CD79a andCD79b contain an immunoreceptor tyrosine-based activation motif in their intracellular tails that used to propagate a signal in a B cell...
January 12, 2019: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30648624/identification-and-expression-profiling-analysis-of-micrornas-in-nile-tilapia-oreochromis-niloticus-in-response-to-streptococcus-agalactiae-infection
#4
Chengbin Gao, Qiang Fu, Ning Yang, Lin Song, Fenghua Tan, Jiajie Zhu, Chao Li
MicroRNAs (miRNAs) play vital regulatory roles in various biological processes, including in immune responses. Nile tilapia (Oreochromis niloticus) is an important commercial fish species in China. To identify immune-related miRNAs of O. niloticus, 4 libraries from liver during S. agalactiae infection (0 h, 5 h, 50 h, and 7 d) were sequenced by high-throughput sequencing technology in tilapia. We obtained 10,703,531, 11,507,163, 11,180,179 and 13,408,414 clean reads per library, respectively. In our results, a total of 482 miRNAs were identified through bioinformatic analysis, including 220 conserved miRNAs and 262 putative novel miRNAs...
January 12, 2019: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30641187/interleukin-12-receptor-%C3%AE-2-from-grass-carp-molecular-characterization-and-its-involvement-in-aeromonas-hydrophila-induced-intestinal-inflammation
#5
Xue-Hong Song, Jian Tang, Tian-Tian Gao, Xu-Fang Xu, Hui-Xing Yang, Kang Wu, Cai-Gen Yang, Zhong-Qin Cheng, Bing-Yao Sun
Interleukin-12 receptor β2 (IL-12Rβ2) is a signaling subunit of heterodimeric receptors for IL-12 and IL-35. It plays important regulatory functions in the development of Th1 cells and in the expression of inflammatory cytokines in mammals and other higher vertebrates. However, little is known about IL-12Rβ2 in teleost fish. In this work, we have cloned and characterized IL-12Rβ2 from grass carp (Ctenopharyngodon idella). The full-length cDNA of grass carp IL-12Rβ2 is 2875 bp, which encodes a mature protein with 741 amino acids...
January 11, 2019: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30641186/pol-mir-194a-of-japanese-flounder-paralichthys-olivaceus-suppresses-type-i-interferon-response-and-facilitates-edwardsiella-tarda-infection
#6
Xiao-Lu Guan, Bao-Cun Zhang, Li Sun
MicroRNAs (miRNAs) are a type of small non-coding RNAs that participate in diverse cellular processes including microbial invasion and immune defense. In a previous study, we identified a large amount of Japanese flounder (Paralichthys olivaceus) miRNAs responsive to megalocytivirus infection. In the present study, we examined the function of one of these miRNAs, pol-miR-194a, in association with the infectivity of Edwardsiella tarda, an intracellular bacterial pathogen to many fish species including flounder...
January 11, 2019: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30641185/host-defense-peptide-leap-2-contributes-to-monocyte-macrophage-polarization-in-barbel-steed-hemibarbus-labeo
#7
Jie Chen, Yao-Ping Lv, Qing-Min Dai, Ze-Hui Hu, Zi-Ming Liu, Ji-Heng Li
The liver-expressed antimicrobial peptide 2 (LEAP-2) plays a vital role in host immunity against pathogenic organisms. In the present study, cDNA of the LEAP-2 gene was cloned and sequenced from the barbel steed (Hemibarbus labeo). The predicted amino acid sequence of the barbel steed LEAP-2 comprises a signal peptide and a prodomain, which is followed by the mature peptide. Sequence analysis revealed that barbel steed LEAP-2 belongs to the fish LEAP-2A cluster and that it is closely related to zebrafish LEAP-2A...
January 11, 2019: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30639866/identification-of-a-novel-c1q-complement-component-in-razor-clam-sinonovacula-constricta-and-its-role-in-antibacterial-activity
#8
Yan Li, Donghong Niu, Yuqi Bai, Tianyi Lan, Maoxiao Peng, Zhiguo Dong, Jiale Li
The serum complement component C1q mediates a variety of immune regulatory functions. Herein, we identified a globular head C1q (ghC1q) gene in razor clam Sinonovacula constricta. The complete Sc-ghC1q gene was 872 bp long included an 81 bp 5'-untranslated region (UTR), a 95 bp 3'-UTR with a poly(A) tail, and an open reading frame (ORF) of 696 bp. The mRNA expression of Sc-ghC1q was upregulated in hepatopancreas and hemocytes. After Staphylococcus aureus or Vibrio anguillarum challenge, Sc-ghC1q mRNA transcript abundance was significantly upregulated in hemolymph...
January 10, 2019: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30639479/antimicrobial-and-biochemical-characterization-of-a-c-type-lectin-isolated-from-pearl-spot-etroplus-suratensis
#9
Abdul Salam Rubeena, Mani Divya, Baskaralingam Vaseeharan, Sivashanmugam Karthikeyan, Einar Ringø, Elumalai Preetham
The present study reveals purification and characterization of a C-type lectin from the serum of pearl spot, Etroplus suratensis (Es-Lec). The Es-Lec was purified by affinity chromatography with mannose coupled sepharose CL-4B column and it exhibits single band with a molecular weight of 75 kDa in SDS-PAGE. The surface morphology of purified Es-Lec displays the homogeneous nature of protein. A distinct peak with a retention time of 2.958 min was appeared in high performance liquid chromatography (HPLC), X-ray diffraction (XRD) analysis expresses a single peak at 31...
January 10, 2019: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30639478/a-role-of-small-gtpase-rac-1-in-immune-response-and-its-interaction-with-pak-1-in-crayfish-procambarus-clarkii
#10
Yuanyuan Feng, Maolin Ma, Xiaojiao Zhang, Die Liu, Lei Wang, Cen Qian, Guoqing Wei, Baojian Zhu
Ras-related C3 botulinum toxin substrate 1 (Rac1) participates in many biological processes. In this study, a Rac1 gene was identified in the crayfish Procambarus clarkii with an open reading frame of 579 bp that encoded 192 amino acids. This predicted 21.4 kDa protein was highly homologous to those in other invertebrates. Real-time PCR analysis revealed that Pc-Rac1 was expressed in all examined tissues with the highest expression level in hemocytes. The transcriptional expression level of Pc-Rac1 was significantly upregulated in hemocytes and hepatopancreas after lipopolysaccharide (LPS) or polyinosinic: polycytidylic acid (poly I: C) induction...
January 9, 2019: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30639477/innate-immune-responses-and-metabolic-alterations-of-mud-crab-scylla-paramamosain-in-response-to-vibrio-parahaemolyticus-infection
#11
Xusheng Zhang, Xixiang Tang, Ngoc Tuan Tran, Ying Huang, Yi Gong, Yueling Zhang, Huaiping Zheng, Hongyu Ma, Shengkang Li
Vibrio parahaemolyticus is one of the major pathogens caused diseases in cultured mud crab (Scylla paramamosain). Mud crabs lack an adaptive immune system, their defenses depend almost on innate immunity. Evaluation of the molecular responses of mud crabs to pathogens is essential for control of disease occurrence in farmed animals. In this study, the impacts of V. parahaemolyticus on immunity-related genes and metabolites in mud crabs of different groups (PG, SG and MG refer to controlled, survival and moribund groups, respectively) were investigated...
January 9, 2019: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30633962/expression-and-localization-study-of-pigr-in-the-late-stage-of-embryo-development-in-turbot-scophthalmus-maximus
#12
Zhihua Qin, Xiaodong Liu, Zekun Yu, Zhibin Sun, Jian Li, Changtao Guan, Jilin Lei, Aijun Ma, Hu Shan
The receptor responsible for maternofetal transmission of immunoglobulin (Igs) in the teleosts is not clear. Polymeric immunoglobulin receptor (pIgR) specifically binds with IgA and IgM and mediates the transcytosis of intracellular polymeric immunoglobulins (pIgs) at the mucosal surface to protect against pathogens. Hence there is a possibility that it may be involved in the transmission of maternal Igs. The aim of the present study was to detect the expression and localization of pIgR during embryonal development in turbot (Scophthalmus maximus)...
January 8, 2019: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30633961/cgaatase-with-specific-expression-pattern-can-be-used-as-a-potential-surface-marker-for-oyster-granulocytes
#13
Miren Dong, Xiaorui Song, Min Wang, Weilin Wang, Peng Zhang, Yu Liu, Meijia Li, Lingling Wang, Linsheng Song
Granulocytes are known as the main immunocompetent hemocytes that play important roles in the immune defense of oyster Crassostrea gigas. In the present study, an alcohol acyltransferase (designed as CgAATase) with specific expression pattern was identified from oyster C. gigas, and it could be employed as a potential marker for the isolation of oyster granulocytes. The open reading frame (ORF) of CgAATase was of 1431 bp, encoding a peptide of 476 amino acids with a typically conserved AATase domain. The mRNA transcripts of CgAATase were highest expressed in hemocytes, lower expressed in hepatopancreas, mantle, gonad, gill, ganglion, adductor muscle, and labial palp...
January 8, 2019: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30630050/effectiveness-of-traditional-chinese-herbal-medicine-san-huang-san-in-combination-with-enrofloxacin-to-treat-ahpnd-causing-strain-of-vibrio-parahaemolyticus-infection-in-litopenaeus-vannamei
#14
Qianqian Zhai, Jian Li
The effects of oral administration of enrofloxacin (ENR) and San-Huang-San (SHS), singly or in combination, on the survival performance, disease resistance, and immunity of Litopenaeus vannamei were investigated. After challenge with an AHPND-causing strain of Vibrio parahaemolyticus (VPAHPND ), shrimp were immediately fed a drug-free diet, diets containing only ENR (20 mg kg-1 ) or SHS (500 mg kg-1 ) or diets containing low-dose (10 mg kg-1 ENR + 250 mg kg-1 SHS), medium-dose (20 mg kg-1 ENR + 500 mg kg-1 SHS), and high-dose (40 mg kg-1 ENR + 1000 mg kg-1 SHS) drug combinations for 5 days...
January 7, 2019: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30630049/molecular-characterization-phylogenetic-analysis-and-adjuvant-effect-of-channel-catfish-interleukin-1%C3%AE-s-against-streptococcus-iniae
#15
Erlong Wang, Tao Liu, Jie Wu, Kaiyu Wang, Defang Chen, Yi Geng, Xiaoli Huang, Ping Ouyang, Weimin Lai, Xiaohui Ai
Channel catfish is one of the most extensively cultured species worldwide, which is widely used as a classical model for comparative immunology. Interleukin-1β (IL1β) is an immunoregulatory cytokine with the potential to enhance the immune response induced by vaccines in many animals. To characterize the molecular characterization and identify the immunoadjuvant role of channel catfish IL1β, molecular cloning, phylogenetic analysis, and expression of two IL1β genes were performed, the bioactivity of their recombinant proteins (rIL1β1 and rIL1β2) were detected in vitro and their adjuvant effects on a subunit vaccine encoding C5a peptidase (pSCPI) of Streptococcus iniae were evaluated...
January 7, 2019: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30630048/identification-and-expression-analysis-of-il-4-13-receptors-in-grass-carp-ctenopharyngodon-idella
#16
Xinyu Jiang, Jingduo Gao, Yujie Xue, Yuting Qin, Xia Li, Zhaosheng Sun, Haixia Xie, Mingxian Chang, Pin Nie, Jun Zou, Qian Gao
Interleukin (IL)-4 and IL-13 are T helper 2 (Th2) cytokines with pleiotropic functions. IL-4 interacts with two receptors consisting of IL-4Rα/γ chain receptor (γC) and IL-4Rα/IL-13Rα1. In contrast, IL-13 binds to IL-13Rα2 but also shares the receptor complex containing IL-4Rα/IL-13Rα1. In fish, two IL-4/13 homologs have been identified but their phylogenetic relationships with IL-4 and IL-13 are ambiguous. In this study, we identified six putative IL-4/13 receptor homologs in grass carp, including γC1, γC2, IL-4Rα1, IL-13Rα1, IL-13Rα2 and a soluble form of IL-4Rα2...
January 7, 2019: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30630047/identification-and-characterization-of-differentially-expressed-genes-in-hepatopancreas-of-oriental-river-prawn-macrobrachium-nipponense-under-nitrite-stress
#17
Jielun Yu, Xiangshan Ji, Xuepeng Wang, Tongming Li, Hui Wang, Qifan Zeng
The oriental river prawn Macrobrachium nipponense is a highly adaptable, tolerant, and fecund freshwater prawn that inhabits a wide range of aquatic environments. The hepatopancreas of crustaceans is not only a site for secretion of digestive enzymes, and also plays important roles in several metabolic processes, such as lipid and carbohydrate metabolism. It is the main organ for the detoxification and immunity. In this study, high-throughput sequencing techniques were used to detect the effect of nitrite stress (10 mg/L nitrite-N for 48 h) on gene expression in the hepatopancreas of M...
January 7, 2019: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30615989/a-novel-c1q-domain-containing-protein-in-black-rockfish-sebastes-schlegelii-serves-as-a-pattern-recognition-receptor-with-immunoregulatory-properties-and-possesses-binding-activity-to-heat-aggregated-igg
#18
Xue Du, Guang-Hua Wang, Bin Yue, Jing-Jing Wang, Qin-Qin Gu, Shun Zhou, Min Zhang, Yong-Hua Hu
C1q-domain-containing (C1qDC) proteins, which are involved in a series of immune responses, are important pattern recognition receptors in innate immunity in vertebrates and invertebrates. Functional studies of C1qDC proteins in vertebrates are scarce. In the present study, a C1qDC protein (SsC1qDC) from the teleost black rockfish (Sebastes schlegelii) was identified and examined at expression and functional levels. The open reading frame of SsC1qDC is 636 bp, and the predicted amino acid sequence of SsC1qDC shares 62%-69% overall identity with the C1qDC proteins of several fish species...
January 4, 2019: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30615988/intelectin-mediated-phagocytosis-and-killing-activity-of-macrophages-in-blunt-snout-bream-megalobrama-amblycephala
#19
Zhujin Ding, Xiaoheng Zhao, Jixiu Wang, Feng Zhang, Weimin Wang, Hong Liu
Intelectin, a lectin discovered recently, has been identified in various vertebrate species, such as fish, amphibians, and mammals. In one of our previous studies, the efficient bacteria binding and agglutinating activity of the recombinant Megalobrama amblycephala intelectin protein (rMamINTL) and the enhanced immunopositive localization have been observed in the hepatic macrophage-like cells (kupffer cells) post Aeromonas hydrophila infection. Thus, the present study primarily focuses on the regulatory effects of rMamINTL on M...
January 4, 2019: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30615987/development-and-potential-use-of-an-edwardsiella-ictaluri-wzz-mutant-as-a-live-attenuated-vaccine-against-enteric-septicemia-in-pangasius-hypophthalmus-tra-catfish
#20
Tran H Triet, Bui T T Tinh, Le V Hau, Tran V Huong, Nguyen-Quoc Binh
Edwardsiella ictaluri is a causative agent of enteric septicemia of catfish (ESC), a seriously lethal disease in Vietnamese catfish (Pangasius hypophthalmus). A safe and effective vaccine against ESC is currently an urgent demand due to antibiotic overuse in pangasius farms has led to an alarming antimicrobial resistance. In this study, two E. ictaluri wzzE mutants (WzM-L3, deficient in a 1038bp-entire wzzE gene and WzM-S3, a 245bp-partial deletion of wzzE) were developed and their protection efficiacy was evaluated in hatched pangasius against ESC by immersion vaccination...
January 4, 2019: Fish & Shellfish Immunology
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