journal
MENU ▼
Read by QxMD icon Read
search

Fish & Shellfish Immunology

journal
https://www.readbyqxmd.com/read/28803959/thymosin-%C3%AE-4-is-involved-in-the-antimicrobial-immune-response-of-golden-pompano-trachinotus-ovatus
#1
Yun Sun, Xiaojuan Chen, Yue Xu, Qiaohong Liu, Xue Jiang, Shifeng Wang, Weiliang Guo, Yongcan Zhou
Thymosin beta belongs to the thymosin family, which consists of a series of highly conserved peptides involved in various biological processes. In teleosts, understanding of the immunological functions of thymosin beta is limited, particularly in vivo, which is essentially unknown. In the current study, we cloned and identified thymosin beta 4 from the teleost fish Golden pompano (Trachinotus ovatus), which we have named TroTβ4. We investigated the expression patterns and functions of TroTβ4 in both in vivo and in vitro assays...
August 10, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28803958/identification-and-characterization-of-lymph-organ-micrornas-in-red-swamp-crayfish-procambarus-clarkii-infected-with-white-spot-syndrome-virus
#2
Zhi-Qiang Du, Xiao-Yun Leng, Xiu-Li Shen, Yan-Hui Jin, Xin-Cang Li
MicroRNAs (miRNAs) were important post-transcriptional regulators and played vital roles in innate immunity system of invertebrates, especially in the aspect of antivirus. In this study, using high-throughput small RNAs Illumina sequencing system, differently expressed miRNAs (DEMs) from lymph organs in red swamp crayfish, Procambarus clarkii, infected with white spot syndrome virus, were identified. As a result, 32 known miRNAs and 7 novel miRNAs were identified in crayfish lymph organ small RNAs library of NG and WG...
August 10, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28797638/a-novel-tnfaip8-gene-mediates-l-arginine-metabolism-in-apostichopus-japonicus
#3
Yina Shao, Huahui Chen, Miao Lv, Chenghua Li, Weiwei Zhang, Ye Li, Xuelin Zhao, Yongbo Bao
Tumor necrosis factor (TNF)-α-induced protein 8 (TNFAIP8) family is a newly identified protein with vital roles in maintaining immune homeostasis. In the current study, we first cloned and characterized a TNFAIP8 gene from the invertebrate sea cucumber Apostichopus japonicus. The gene was designated as AjTNFAIP8. The full-length cDNA of AjTNFAIP8 was 1455 bp long and encoded a matured protein of 201 amino acid residues. Structural analysis indicated that AjTNFAIP8 had a death effector domain (DED)-like domain and composed of six α-helices...
August 7, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28797637/temporal-changes-in-innate-immunity-parameters-epinecidin-gene-expression-and-mortality-in-orange-spotted-grouper-epinephelus-coioides-experimentally-infected-with-a-fish-pathogen-vibrio-harveyi-jml1
#4
Edgar C Amar, Joseph P Faisan, Mary Jane S Apines-Amar, Rolando V Pakingking
Changes in innate immunity parameters and epinecidin mRNA transcript levels were examined to characterize the non-specific immune response of E. coioides to pathogenic V. harveyi JML1 isolated from affected cage-cultured fish. After fish had been injected with bacteria at a dose causing 30% mortality, blood and tissue samples were collected at 0, 6, 12, 24, 48, 72, 96, 120, and 240 h post-infection (hpi) for assessment of indices such as the oxidative burst (OB) and phagocytic index (PI) of head kidney cells, and lysozyme activity (LYS) and total immunoglobulin (Total Ig) levels of the plasma...
August 7, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28774847/dietary-supplementation-of-organic-selenium-improves-growth-survival-antioxidant-and-immune-status-of-meagre-argyrosomus-regius-juveniles
#5
Abdallah Tag-Eldein Mansour, Ashraf Abdelsamee Goda, Eglal Ali Omar, Hala Saber Khalil, Maria Ángeles Esteban
Oxidative stress is considered one of the main obstacles to immune competence and high mortality rate of marine fish larvae due to the high dietary polyunsaturated fatty acids and accelerated metabolic rate. This study was carried out to assess the effect of different dietary selenium (Se) yeast on growth, antioxidant status, hematological changes, and cellular and humoral immune parameters in meagre (Argyrosomus regius). Juvenile specimens (3.20 ± 0.17 g) were randomly assigned to four experimental groups, a control group fed a basal diet and another three groups fed Se-supplemented diets at doses of 1, 2 and 3 mg Se-yeast kg(-1) diet for 63 days and the final analyzed selenium concentrations were 0...
July 31, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28774846/inhibited-fatty-acid-%C3%AE-oxidation-impairs-stress-resistance-ability-in-nile-tilapia-oreochromis-niloticus
#6
Han Pan, Ling-Yu Li, Jia-Min Li, Wei-Li Wang, Samwel Mchele Limbu, Pascal Degrace, Dong-Liang Li, Zhen-Yu Du
Energy metabolism plays important roles in stress resistance and immunity in mammals, however, such functions have not been established in fish. In the present study, Nile tilapia (Oreochromis niloticus) was fed with mildronate, an inhibitor of mitochondrial fatty acid (FA) β-oxidation, for six weeks subsequently challenged with Aeromonas hydrophila and ammonia nitrogen exposure. Mildronate treatment reduced significantly l-carnitine concentration and mitochondrial FA β-oxidation efficiency, while it increased lipid accumulation in liver...
July 31, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28764985/function-of-two-ficolin-like-proteins-in-innate-immune-defense-of-the-oriental-river-prawn-macrobrachium-nipponense
#7
Tingting Li, Lei Wu, Min Jin, Futong Ma, Xin Huang, Qian Ren
Ficolins have crucial functions in recognizing and eliminating pathogens in innate immunity. In this study, we identified two ficolin-like genes from the oriental river prawn, Macrobrachium nipponense. These genes were designated as MnFico1 and MnFico2. MnFico1 cDNA has 1600 bp, whereas MnFico2 has 1486 bp. In addition to a coiled-coil region or a low complexity region, the two ficolins both contained a signal peptide and a fibrinogen-related domain. qRT-PCR results showed that the highest expression level of MnFico1 expression was in the gills, whereas that of MnFico2 was in the heart...
July 29, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28757200/silver-nanoparticles-enhance-wound-healing-in-zebrafish-danio-rerio
#8
Seung Beom Seo, S H S Dananjaya, Chamilani Nikapitiya, Bae Keun Park, Ravi Gooneratne, Tae-Yoon Kim, Jehee Lee, Cheol-Hee Kim, Mahanama De Zoysa
Silver nanoparticles (AgNPs) were successfully synthesized by a chemical reduction method, physico-chemically characterized and their effect on wound-healing activity in zebrafish was investigated. The prepared AgNPs were circular-shaped, water soluble with average diameter and zeta potential of 72.66 nm and -0.45 mv, respectively. Following the creation of a laser skin wound on zebrafish, the effect of AgNPs on wound-healing activity was tested by two methods, direct skin application (2 μg/wound) and immersion in a solution of AgNPs and water (50 μg/L)...
July 27, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28757199/comparative-transcriptome-analysis-of-zebrafish-danio-rerio-brain-and-spleen-infected-with-spring-viremia-of-carp-virus-svcv
#9
Yeda Wang, Hao Zhang, Yuanan Lu, Fang Wang, Liyue Liu, Jingxia Liu, Xueqin Liu
Spring viremia of carp virus (SVCV) is the pathogen of spring viremia of carp (SVC) and often causes acute hemorrhagic symptoms in various kinds of cyprinids and induces serious environmental and economic losses. However, the molecular mechanisms of infection remain poorly understood, especially at the individual level. In this study, zebrafish was employed as the infection model to explore the pathogenesis of SVCV. 4 groups of zebrafish tissues were set and RNA sequencing (RNA-Seq) technology was employed to analyze the differentially expressed genes (DEGs) after SVCV-infection...
July 27, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28757198/dissecting-the-immune-pathways-stimulated-following-injection-vaccination-of-rainbow-trout-oncorhynchus-mykiss-against-enteric-redmouth-disease-erm
#10
Eakapol Wangkahart, Christopher J Secombes, Tiehui Wang
Enteric redmouth disease (ERM or yersiniosis) is one of the most important diseases of salmonids and leads to significant economic losses. It is caused by the Gram-negative bacterium Yersinia ruckeri but can be controlled by bacterin vaccination. The first commercial ERM vaccine was licenced in 1976 and is one of the most significant and successful health practices within the aquaculture industry. Although ERM vaccination provides complete protection, knowledge of the host immune response to the vaccine and the molecular mechanisms that underpin the protection elicited is limited...
July 27, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28756289/generation-of-a-recombinant-viral-hemorrhagic-septicemia-virus-vhsv-expressing-olive-flounder-paralichthys-olivaceus-interferon-%C3%AE-and-its-effects-on-type-i-interferon-response-and-virulence
#11
Jun Soung Kwak, Min Sun Kim, Ki Hong Kim
Rhabdoviruses including viral hemorrhagic septicemia virus (VHSV) are highly susceptible to type I interferon (IFN) responses, and IFN-γ that is belonging to the type II IFN has been known to enhance type I IFN responses in mammals. In this study, we generated a recombinant VHSV that can express olive flounder IFN-γ (rVHSV-A-IFNγ) using reverse genetics technology, and analyzed the effect of rVHSV-A-IFNγ infection on type I IFN response in Epithelioma papulosum cyprini (EPC) cells. Furthermore, the virulence of rVHSV-A-IFNγ was evaluated by infection to olive flounder (Paralichthys olivaceus)...
July 27, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28756288/production-of-rnase-iii-knockout-auxotrophic-edwardsiella-tarda-mutant-for-delivery-of-long-double-stranded-rna-and-evaluation-of-its-immunostimulatory-potential
#12
Min Sun Kim, Seung Hyuk Choi, Jeong In Yang, Ki Hong Kim
The artificially synthesized polyinosinic-polycytidylic acid (poly IC) has been widely used to induce type I IFN responses in various vertebrates including fish. However, as poly IC is too expensive to use in aquaculture, the development of another economical long dsRNA producing method is needed to practically use long dsRNAs in aquaculture farms for the control of infectious diseases. In the present study, to produce long dsRNAs economically, we developed a novel long dsRNA production system based on the RNase III gene deleted auxotrophic mutant E...
July 26, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28756287/interplay-between-the-gut-microbiota-and-immune-responses-of-ayu-plecoglossus-altivelis-during-vibrio-anguillarum-infection
#13
Li Nie, Qian-Jin Zhou, Yan Qiao, Jiong Chen
Gut microbiota plays fundamental roles in protection against pathogen invasion. However, the mechanism and extent of responses of gut microbiota to pathogenic infection are poorly understood. This study investigated the gut bacterial communities and immune responses of ayu (Plecoglossus altivelis) upon exposure to Vibrio anguillarum. The succession of V. anguillarum infection was evidenced by increased expression of immune genes and bacterial loads in ayu tissues, which in turn altered the composition and predicted functions of gut bacterial community...
July 26, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28754613/growth-performance-innate-immune-responses-and-disease-resistance-of-fingerling-blunt-snout-bream-megalobrama-amblycephala-adapted-to-different-berberine-dietary-feeding-modes
#14
Wei-Na Xu, Dan-Hong Chen, Qing-Qing Chen, Wen-Bin Liu
A 8-week feeding trial was conducted to evaluate the effect of different berberine-dietary feeding modes on growth, non-specific immune responses and disease resistance of blunt snout bream, Megalobrama amblycephala. Fish (average initial weight 4.70 ± 0.02 g) were fed two fat levels (5% and 10%) diets in three berberine-feeding modes (supplementing 50 mg/kg berberine continuously, two-week or four-week intervals) with four replicates, respectively. Then, fish were challenged by Aeromonas hydrophila and mortality was recorded for the next 96 h after feeding trial...
July 25, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28751272/effect-of-feeding-frequency-on-growth-body-composition-antioxidant-status-and-mrna-expression-of-immunodependent-genes-before-or-after-ammonia-n-stress-in-juvenile-oriental-river-prawn-macrobrachium-nipponense
#15
Zhili Ding, Youqin Kong, Yixiang Zhang, Jingfen Li, Fang Cao, Junbo Zhou, Jinyun Ye
Feeding frequency is important for the improvement of growth performance and immunity of aquatic animals. In this study, the effect of feeding frequency on growth, body composition, antioxidant status and mRNA expression of immunodependent genes before or after ammonia-N stress was examined in Macrobrachium nipponense. Prawns were randomly assigned to one of five feeding frequencies (1, 2, 3, 4 and 6 times/day) following the same ration size over an 8-week growth trial. After the feeding trial, prawns were challenged by ammonia-N...
July 24, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28743630/effect-of-cucurbita-mixta-l-seed-meal-enrichment-diet-on-growth-immune-response-and-disease-resistance-in-oreochromis-mossambicus
#16
Mohamed Saiyad Musthafa, Abdul Rahman Jawahar Ali, Meenatchi Sundaram Arun Kumar, Bilal Ahmad Paray, Mohammad K Al-Sadoon, Chellam Balasundaram, Ramasamy Harikrishnan
The impact of Cucurbita mixta (L.) seed meal enriched diet on growth performance, innate immune response, and disease resistance in Oreochromis mossambicus against Aeromonas hydrophila was investigated. O. mossambicus was fed with 2 g kg(-1), 4 g kg(-1), and 6 g kg(-1) C. mixta seed meal diets for a period of 4 weeks. The results indicated that C. mixta seed meal diets at 4 g kg(-1) and 6 g kg(-1) significantly (P < 0.05) enhances the survival rate, weight gain (WG), protein efficiency ratio (PER), specific growth rate (SGR), feed conversion ratio (FCR), and feed efficiency (FE) from weeks 1-4 when compared to control...
July 23, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28743627/characterization-of-a-novel-non-receptor-tyrosine-kinase-src-from-litopenaeus-vannamei-and-its-response-to-white-spot-syndrome-virus-infection
#17
Defu Yao, Lingwei Ruan, Jingxiang Xu, Hong Shi, Xun Xu
Src family kinases (SFKs), a class of non-receptor tyrosine kinases, mediate a wide aspect of cellular signaling pathways that regulate cell proliferation, differentiation, motility and survival. In this study, we identified and characterized for the first time a novel SFK homologue from Litopenaeus vannamei (designated as LvSrc). Sequence analysis showed that LvSrc had a high homology with the identified SFKs, especially those from invertebrates. LvSrc contained the conserved SH3, SH2 and tyrosine kinase domains, as well as the potential phosphorylation and lipid modification sites...
July 23, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28743632/comparative-analysis-of-dual-specificity-protein-phosphatase-genes-1-2-and-5-in-response-to-immune-challenges-in-japanese-flounder-paralichthys-olivaceus
#18
Shuo Li, Gaixiang Hao, Jiafang Li, Weijiao Peng, Xuyun Geng, Jinsheng Sun
Dual-specificity MAP kinase (MAPK) phosphatases (DUSPs) are well-established negative modulators in regulating MAPK signaling in mammalian cells and tissues. Our previous studies have shown the involvement of DUSP6 in regulating innate immunity in Japanese flounder Paralichthys olivaceus. In order to gain a better understanding of the role of DUSPs in fish innate immunity, in the present study we identified and characterized three additional DUSP genes including DUSP1, 2 and 5 in P. olivaceus. The three Japanese flounder DUSP proteins share common domain structures composed of a conserved N-terminal Rhodanase/CDC25 domain and a C-terminal catalytic phosphatase domain, while they show only less than 26% sequence identities, indicating that they may have different substrate selectivity...
July 22, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28743631/molecular-characterization-and-functional-analysis-of-the-hepcidin-gene-from-roughskin-sculpin-trachidermus-fasciatus
#19
Yingying Liu, Xiaodi Han, Xuezhao Chen, Shanshan Yu, Yingmei Chai, Tongjie Zhai, Qian Zhu
Hepcidin is a kind of cysteine-rich antimicrobial peptide that plays a vital role in host innate immune activity and iron regulation. Here, we report the molecular characterization and functional analysis of a novel hamp1 hepcidin isoforms Tf-Hep from roughskin sculpin, Trachidermus fasciatus. A cDNA fragment of 988 bp with an ORF of 273 bp was obtained. The coding sequence encodes for a signal peptide of 24 amino acids coupled with a prodomain of 40 amino acids and a mature peptide of 26 amino acids. Tissue distribution analysis indicated that Tf-Hep was most abundant in the liver...
July 22, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28743629/administration-of-a-polyphenol-enriched-feed-to-farmed-sea-bass-dicentrarchus-labrax-l-kidney-melanomacrophages-response
#20
Marcella Arciuli, Daniela Fiocco, Sergio Fontana, Mattia Pia Arena, Maria Antonia Frassanito, Anna Gallone
The reinforcement of the defense mechanism of fish, through the administration of immunostimulants, is considered as a promising alternative to vaccines. Natural immunostimulants such as polyphenols, flavanoids, pigments and essential oils can modulate the innate immune response. In lower vertebrates, melano-macrophage centres, i.e. clusters of pigment-containing cells forming the extracutaneous pigment system, are wide-spread in the stroma of the haemopoietic tissue, mainly in kidney and spleen. In fishes, melano-macrophage centres play an important role in the immune response against antigenic stimulants and pathogens...
July 22, 2017: Fish & Shellfish Immunology
journal
journal
32215
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"