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Fish & Shellfish Immunology

Luz Natalia Franco Montoya, Gisele Cristina Favero, Fabio Sabbadin Zanuzzo, Elisabeth Criscuolo Urbinati
This study investigated the effects of two β-glucan molecules with different purities and isolated by different biotechnological processes on the immune response of matrinxã (Brycon amazonicus) prior and after challenge with Aeromonas hydrophila. In this sense, we evaluated serum cortisol and plasma glucose levels, the number of leukocytes (lymphocytes, neutrophils and monocytes), as well as the respiratory activity of leukocytes prior to, 6 and 24 h post infection (hpi). During 15 days, fish were fed with diets containing 0...
September 13, 2018: Fish & Shellfish Immunology
Kai Luo, Jun Di, Panpan Han, Shuhuan Zhang, Lihai Xia, Guangming Tian, Wenbing Zhang, Dan Dun, Qiaoqing Xu, Qiwei Wei
Dabry's sturgeon (Acipenser dabryanus), as a living fossil, is considered a critically endangered aquatic animal in China. To date, the immune system of this species remains largely unknown, with limited available sequence information. In addition, increasing incidence of bacterial pathogenic diseases has been reported. Hence, the present study aimed to characterize comprehensively transcriptome profile of the head kidney from Dabry's sturgeon infected with Aeromonas hydrophila using Illumina platform. Over 42 million high-quality reads were obtained and de novo assembled into a final set of 195240 unique transcript fragments (unigenes), with an average length of 564 bp...
September 13, 2018: Fish & Shellfish Immunology
Xupeng Li, Xianhong Meng, Sheng Luan, Kun Luo, Baoxiang Cao, Baolong Chen, Jie Kong
Raf is a member in the Ras/Raf/MAPKK/MAPK signaling transduction pathway. To obtain a better understanding of Raf in the interaction between the Chinese shrimp Fenneropenaeus chinensis and white spot syndrome virus (WSSV), the sequence of cDNA of Raf from F. chinensis (FcRaf) was obtained. The FcRaf gene contained a 2421 bp open reading frame (ORF). The FcRaf shared most characteristic of Raf protein, such as the Raf-like Ras-binding domain (RBD), phorbol esters/diacylglycerol binding domain (C1 domain), and catalytic domain of the serine/threonine kinases, Raf (STKc_Raf)...
September 13, 2018: Fish & Shellfish Immunology
Liqing Zhou, Dan Zhao, Biao Wu, Xiujun Sun, Zhihong Liu, Feng Zhao, Zhenming Lv, Aiguo Yang, Qing Zhao, Gaowei Zhang, Chunyan Ma
Scapharca broughtonii is one of the most important Arcidae aquaculture species in the Asia-Pacific region. We aimed to investigate the immune responses of hemocytes from ark shell S. broughtonii hemolymph against pathogens. Hemocyte ultrastructure and immunological activity in response to Vibrio anguillarum challenge were observed by scanning and transmission electron microscopy. Before ultrastructure observation, we used the API ZYM semi-quantitative kit to evaluate the levels of hydrolytic enzymes in the plasma and hemocytes following V...
September 13, 2018: Fish & Shellfish Immunology
M A Henry, F Gai, P Enes, A Peréz-Jiménez, L Gasco
The present study investigated the effects of 25 and 50% dietary inclusion of yellow mealworm, Tenebrio molitor (TM), larvae on the intestinal antioxidant enzymes, lipid peroxidation and immune system of rainbow trout, Oncorhynchus mykiss. Fish were fed for 90 days with a control diet (TM0) or with 2 test diets (TM25 and TM50) where 35 and 67% of the fishmeal was replaced by TM larvae meal respectively. At the end of the growth trial, humoral immune parameters were assessed in the sera of the fish and the activities of antioxidant enzymes were measured in intestine homogenates...
September 13, 2018: Fish & Shellfish Immunology
Matt D Johansen, Elinor Hortle, Joshua A Kasparian, Alejandro Romero, Beatriz Novoa, Antonio Figueras, Warwick J Britton, Kumudika de Silva, Auriol C Purdie, Stefan H Oehlers
Changes to lipid metabolism are well-characterised consequences of human tuberculosis infection but their functional relevance are not clearly elucidated in these or other host-mycobacterial systems. The zebrafish-Mycobacterium marinum infection model is used extensively to model many aspects of human-M. tuberculosis pathogenesis but has not been widely used to study the role of infection-induced lipid metabolism. We find mammalian mycobacterial infection-induced alterations in host Low Density Lipoprotein metabolism are conserved in the zebrafish model of mycobacterial pathogenesis...
September 13, 2018: Fish & Shellfish Immunology
Valerio Matozzo, Maria Gabriella Marin, Luciano Masiero, Martina Tremonti, Sara Biamonte, Silvia Viale, Livio Finos, Giulio Lovato, Paolo Pastore, Sara Bogialli
The effects of the breakdown products of herbicides on aquatic species are largely unknown. In a recent study, we evaluated the effects of glyphosate on the mussel Mytilus galloprovincialis. This study was designed to evaluate for the first time the impact of aminomethylphosphonic acid (AMPA) - the main breakdown product of glyphosate - on cellular and biochemical parameters of the mussel Mytilus galloprovincialis. Bivalves were exposed for 7, 14 and 21 days to 1, 10 and 100 μg/L of AMPA and various biomarkers were measured in haemolymph (total haemocyte counts, haemocyte diameter and volume, haemolymph pH, haemocyte proliferation, haemolymph lactate dehydrogenase activity, haemocyte lysate lysozyme and acid phosphatase activities), as well as in gills and digestive gland (superoxide dismutase, catalase, glutathione S-transferase and acetylcholinesterase activities)...
September 12, 2018: Fish & Shellfish Immunology
Shengzhen Jin, Xin Zhao, Hongquan Wang, Jianming Su, Jing'an Wang, Chunhua Ding, Yaoguo Li, Tiaoyi Xiao
The barbel chub (Squaliobarbus curriculus) is a kind of small size commercial fish species that is widely spread in Asia and has shown significant resistance to disease. In this study, the full-length cDNA sequences of Toll-like receptor (TLR) 7 and 8 from S. curriculus, designated as ScTLR7 and ScTLR8, were cloned, and their differences in the structure and the responses to the grass carp (GCRV) infection and lipopolysaccharide stimulation were investigated. The full-length 3715 base pair (bp) cDNA of ScTLR7 contained a complete open reading frame of 3162 bp and encoded a putative polypeptide of 1053 amino acid residues...
September 12, 2018: Fish & Shellfish Immunology
Seunghan Lee, Kumar Katya, Ali Hamidoghli, Jeongwhui Hong, Dae-Jung Kim, Sungchul C Bai
This study evaluated the synergistic effects of dietary Bacillus subtilis WB60 and mannanoligosaccharide (MOS) in juvenile Japanese eel, Anguilla japonica. Seven treatment diets were formulated to contain three different levels of B. subtilis (0.0, 0.5, and 1.0 × 107  CFU/g diet denoted as BS0 , BS0.5 , and BS1 , respectively) with two MOS levels (0 and 5 g/kg diet denoted as M0 and M5 , respectively), and one diet with oxytetracycline (OTC) at 5 g/kg diet. Each diet (BS0 M0 (CON), BS0 M5 , BS0.5 M0, BS0...
September 11, 2018: Fish & Shellfish Immunology
Kexin Duan, Xiaojing Hua, Yuting Wang, Yanxue Wang, Yaping Chen, Wen Shi, Lijie Tang, Yijing Li, Min Liu
Infectious pancreatic necrosis virus (IPNV) infects wild and cultured salmonid fish causing high mortality with serious economic losses to salmonid aquaculture. Ideally, the method of oral immunization should prevent the infection of rainbow trout juveniles with IPNV. In the present study, genetically engineered Lactobacillus casei 393 pPG-612-VP2/L. casei 393 and pPG-612-CK6-VP2/L. casei 393 constitutively expressing VP2 protein of IPNV were constructed. The recombinant strains pPG-612-CK6-VP2/L. casei 393 and pPG-612-VP2/L...
September 11, 2018: Fish & Shellfish Immunology
Lan Zhang, Luqing Pan, Lijun Xu, Lingjun Si
Immune responses and intracellular signaling pathways were examined after hemolymph of Litopenaeus vannamei being incubated in Crustacean hyperglycemic hormone (CHH), dopamine (DA) and DA antagonist (Y). The results showed that the effect CHH and CHH + DA + Y on viability of hemocytes were no significant changes compared to the control group. However, in DA, DA + Y and CHH + DA groups, the viability of hemocytes decreased significantly. The phagocytic activity and the antibacterial activity of CHH group were increased significantly within 12h...
September 11, 2018: Fish & Shellfish Immunology
Yu Liu, Zhao-Zhe Xin, Xiao-Yu Zhu, Ying Wang, Dai-Zhen Zhang, Sen-Hao Jiang, Hua-Bin Zhang, Chun-Lin Zhou, Qiu-Ning Liu, Bo-Ping Tang
The mudflat crab Helice tientsinensis is one of the most economically important aquaculture species in China. Nevertheless, it is susceptible to various diseases caused by viruses, bacteria and rickettsia-like organisms. A better understanding of the immune system and genes related to the responses to bacterial and viral infection is required. Herein, the hepatopancreas transcriptome of H. tientsinensis was analyzed by comparing control and lipopolysaccharide (LPS)-stimulated RNA-Seq data, yielding 91,885,038 bp and 13...
September 11, 2018: Fish & Shellfish Immunology
Xin Huang, Ling-Hong Miao, Yan Lin, Wen-Jing Pan, Ming-Chun Ren, Xian-Ping Ge, Bo Liu, Qun-Lan Zhou
High glucose levels are known to impair growth and immune function in fish. Here we investigated the role of glucose-6-phosphate dehydrogenase (G6PD) and NADPH oxidase (NOX) in high glucose-associated impairment of leukocyte respiratory burst activity in Megalobrama amblycephala. We cultured peripheral leukocytes isolated from M. amblycephala with media containing no glucose (non-glucose group), 11.1 mmol/L d-glucose (physiologic glucose group), 22.2 mmol/L d-glucose (high-glucose group), or 11.1 mmol/L d-glucose + 100 μmol/L dehydroepiandrosterone (DHEA) (DHEA-treated group)...
September 10, 2018: Fish & Shellfish Immunology
Juan-Juan Li, Yun Zhang, Li-Wen Han, Qing-Ping Tian, Qiu-Xia He, Xi-Min Wang, Chen Sun, Jian Han, Ke-Chun Liu
Marsdenia tenacissima exhibits biological activity with heat-clearing and detoxifying properties, relieving coughs and asthma and exerting anticancer and anti-HIV effects. Tenacissioside H (TH) is a Chinese medicine monomer extracted from the dried stem of Marsdenia tenacissima. We investigated the in vivo anti-inflammatory activity of TH using three different zebrafish inflammation models: local inflammation induced by tail cutting, acute inflammation induced by CuSO4, and systemic inflammation induced by lipopolysaccharide (LPS)...
September 10, 2018: Fish & Shellfish Immunology
Baoying Guo, Shuobo Liu, Jiji Li, Zhi Liao, Huihui Liu, Hu Xia, Pengzhi Qi
Myeloid differentiation factor 88 (MyD88) is a pivotal adapter protein that involved in interleukin-1 receptor/toll-like receptor (IL-1R/TLR) signal transduction, which could spur downstream cascades and eventually drawn into innate immune response. MyD88 has been extensively studied in vertebrates, however, the information ascribe to MyD88 in invertebrates is still very scarce especially its function annotation remains extremely obscure. At here, three novel MyD88 isoforms termed McMyD88a, McMyD88b and McMyD88c were firstly cloned from thick shell mussel Mytilus coruscus...
September 8, 2018: Fish & Shellfish Immunology
Guo Qiao, Mingming Zhang, Ye Li, Chen Xu, De-Hai Xu, Zhigang Zhao, Jialin Zhang, Qiang Li
Gibel carp (Carassius auratus gibelio), a major aquaculture species in China, has emerged in a seriously epizootic disease caused by Cyprinid herpesvirus 2 (CyHV-2). There are no effective methods to prevent or control this serious disease. Biofloc technology (BFT) can improve water quality, reduce pathogens introduction, enhance cultured species immunity and disease resistance. In this study, a 30-day experiment was conducted to investigate the effect of BFT on innate immune response and disease resistance of gibel carp against CyHV-2 infection...
September 8, 2018: Fish & Shellfish Immunology
Fei Zhu, Xiyi Qian, Ziyan Wang
The minichromosome maintenance protein (MCM7) is a member of the MCM protein family which participates in the MCM complex by playing a role in the cell replication cycle and chromosome initiation in eukaryotes. The 2270 bp cDNA sequence of MCM7, including a 2127-bp open reading frame (ORF) encoding a 709-aa protein, was cloned from Scylla paramamosain using RT-PCR and RACE. Data showed that MCM7 was highly expressed in the digestive organ and hepatopancreas of S. paramamosain. Furthermore, MCM7 expression was down-regulated by infection with white spot syndrome virus (WSSV) or Vibrio alginolyticus...
September 8, 2018: Fish & Shellfish Immunology
Yundong Li, Falin Zhou, Jianhua Huang, Lishi Yang, Song Jiang, Qibin Yang, Jianguo He, Shigui Jiang
Ammonia is a major aquatic environmental pollutant that negatively impacts shrimp health and commercial productivity. However, we currently do not fully understand the underlying molecular mechanisms of ammonia stress in shrimp. We therefore performed transcriptomic analysis of hepatopancreas from black tiger shrimp (Penaeus monodon) treated with ammonia-stress. We obtained 146,410,174 and 115,241,048 clean reads for the control and treatment groups, respectively. A total of 64,475 unigenes with an average length of 1275 bp and a N50 value of 2158 bp were assembled...
September 8, 2018: Fish & Shellfish Immunology
Nan Chen, Jingjing Jiang, Xiaojian Gao, Xixi Li, Yue Zhang, Xiaodan Liu, Hui Yang, Xuwen Bing, Xiaojun Zhang
Hemorrhagic septicemia of mandarin fish (Siniperca chuatsi) was mainly caused by Aeromonas hydrophila which was an opportunistic pathogen. In recent years, the disease has caused tremendous economic loss with high morbidity and mass mortality in the mandarin fish breeding industry. Histopathological analysis and the immune related gene expression profiles of mandarin fish (S. chuatsi) infected with A. hydrophila were investigated in this study. Transmission electron microscopy (TEM) images showed that the cells of A...
September 7, 2018: Fish & Shellfish Immunology
Wenting Xu, Chenglong Jiao, Pengcheng Bao, Qin Liu, Pengbo Wang, Ruilin Zhang, Xiaohong Liu, Yuanxing Zhang
Turbot (Scophthalmus maximus L.) is a commercially important fish species in China. Despite of its great economic potential, fish farms often suffer severe economic losses due to certain fish diseases. Vaccination has become a common strategy to prevent diseases caused by pathogens in aquaculture industry. However, no inactivated vaccine against Vibrio harveyi of turbot has been reported so far. In this study, we developed an inactivated vaccine using formalin-killed cells of V. harveyi and the efficacy of a commercial adjuvant Montanide™ ISA 763 A VG on the inactivated vaccine was evaluated...
September 7, 2018: Fish & Shellfish Immunology
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