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https://www.readbyqxmd.com/read/28323135/effect-of-bioaugmentation-by-cellulolytic-bacteria-enriched-from-sheep-rumen-on-methane-production-from-wheat-straw
#1
E Gozde Ozbayram, Sabine Kleinsteuber, Marcell Nikolausz, Bahar Ince, Orhan Ince
The aim of this study was to determine the potential of bioaugmentation with cellulolytic rumen microbiota to enhance the anaerobic digestion of lignocellulosic feedstock. An anaerobic cellulolytic culture was enriched from sheep rumen fluid using wheat straw as substrate under mesophilic conditions. To investigate the effects of bioaugmentation on methane production from straw, the enrichment culture was added to batch reactors in proportions of 2% (Set-1) and 4% (Set-2) of the microbial cell number of the standard inoculum slurry...
March 17, 2017: Anaerobe
https://www.readbyqxmd.com/read/28323134/effect-of-operating-temperature-on-anaerobic-digestion-of-the-brazilian-waterweed-egeria-densa-and-its-microbial-community
#2
Keiko Watanabe, Mitsuhiko Koyama, Junko Ueda, Syuhei Ban, Norio Kurosawa, Tatsuki Toda
To develop an effective treatment for the globally invasive Brazilian waterweed Egeria densa, anaerobic digestion was observed at 37 °C, 55 °C, and 65 °C. The average methane production rate at 55 °C was 220 mL L(-1) day(-1), which was two-fold that at 37 °C and 65 °C. Volatile fatty acid accumulation was detected under thermophilic conditions; however, although there was methane production, the system did not shutdown. The microbial communities differed between mesophilic (37 °C) and thermophilic (55 °C and 65 °C) conditions...
March 16, 2017: Anaerobe
https://www.readbyqxmd.com/read/28323133/the-effect-of-penicillin-administration-in-early-life-on-murine-gut-microbiota-and-blood-lymphocyte-subsets
#3
Jaroslaw Daniluk, Urszula Daniluk, Malgorzata Rusak, Milena Dabrowska, Joanna Reszec, Magdalena Garbowicz, Kinga Humińska, Andrzej Dabrowski
BACKGROUND AND AIM: Antibiotics have many beneficial effects but their uncontrolled use may lead to increased risk of serious diseases in the future. Our hypothesis is that an early antibiotic exposition may affect immune system by altering gut microbiota. Therefore, the aim of the study was to determine the effect of penicillin treatment on gut microorganisms and immune system of mice. METHODS: 21-days old C57BL6/J/cmdb male mice were treated with low-dose of penicillin (study group) or water only (control group) for 4 weeks...
March 16, 2017: Anaerobe
https://www.readbyqxmd.com/read/28315382/characterization-of-vaginal-lactobacillus-species-by-rplk-based-multiplex-qpcr-in-russian-women
#4
Vladimir V Demkin, Stanislav I Koshechkin
We describe a multiplex qPCR assay for identification and quantitative assessment of a set of vaginal Lactobacillus species, including L. acidophilus, L. crispatus, L. gasseri, L. helveticus, L. iners, and L. jensenii. The assay extends the previously developed qPCR method for Lactobacillus detection and total quantification based on targeting the rplK gene. Both assays use only single pair of primers and a set of probes combined in three reactions, comprising a vaginal Lactobacillus diagnostic assay panel...
March 14, 2017: Anaerobe
https://www.readbyqxmd.com/read/28300642/distribution-and-phylogeny-of-brachyspira-spp-in-human-intestinal-spirochetosis-revealed-by-fish-and-16s-rrna-gene-analysis
#5
Pablo Rojas, Annett Petrich, Julia Schulze, Alexandra Wiessner, Christoph Loddenkemper, Hans-Jörg Epple, William Sterlacci, Michael Vieth, Judith Kikhney, Annette Moter
During six years as National Reference Laboratory for Spirochetes we investigated 149 intestinal biopsies from 91 patients, which were histopathologically diagnosed with human intestinal spirochetosis (HIS), using fluorescence in situ hybridization (FISH) combined with 16S rRNA gene PCR and sequencing. Aim of this study was to complement histopathological findings with FISH and PCR for definite diagnosis and species identification of the causative pathogens. HIS is characterized by colonization of the colonic mucosa of the human distal intestinal tract by Brachyspira spp...
March 11, 2017: Anaerobe
https://www.readbyqxmd.com/read/28288825/demand-driven-biogas-production-by-flexible-feeding-in-full-scale-process-stability-and-flexibility-potentials
#6
E Mauky, S Weinrich, H F Jacobi, H J Nägele, J Liebetrau, M Nelles
For future energy supply systems with high proportions from renewable energy sources, biogas plants are a promising option to supply demand-driven electricity to compensate the divergence between energy demand and energy supply by uncontrolled sources like wind and solar. Apart expanding gas storage capacity a demand-oriented feeding with the aim of flexible gas production can be an effective alternative. The presented study demonstrated a high degree of intraday flexibility (up to 50% compared to the average) and a potential for an electricity shutdown of up to 3 days (decreasing gas production by more than 60%) by flexible feeding in full-scale...
March 10, 2017: Anaerobe
https://www.readbyqxmd.com/read/28285095/quantitative-proteomic-analysis-of-extracellular-matrix-extracted-from-mono-and-dual-species-biofilms-of-fusobacterium-nucleatum-and-porphyromonas-gingivalis
#7
Marwan Mansoor Ali Mohammed, Veronika Kuchařová Pettersen, Audun H Nerland, Harald G Wiker, Vidar Bakken
The Gram-negative bacteria Fusobacterium nucleatum and Porphyromonas gingivalis are members of a complex dental biofilm associated with periodontal disease. In this study, we cultured F. nucleatum and P. gingivalis as mono- and dual-species biofilms, and analyzed the protein composition of the biofilms extracellular polymeric matrix (EPM) by high-resolution liquid chromatography-tandem mass spectrometry. Label-free quantitative proteomic analysis was used for identification of proteins and sequence-based functional characterization for their classification and prediction of possible roles in EPM...
March 9, 2017: Anaerobe
https://www.readbyqxmd.com/read/28285096/periprosthetic-joint-infection-by-propionibacterium-acnes-clinical-differences-between-monomicrobial-versus-polymicrobial-infection
#8
Raúl Figa, David Muñetón, Lucía Gómez, A Matamala, Mayli Lung, Eva Cuchi, Pablo S Corona
OBJECTIVE: To compare a series of monomicrobial Propionibacterium acnes (P. acnes) knee and hip periprosthetic joint infection (PJI) cases with cases of polymicrobial PJI which included P. acnes. We hypothesized that the presence of a polymicrobial P. acnes infection would lead to worse outcomes than those in cases of monomicrobial P. acnes PJI. METHODS: Retrospective multicentre study. All patients with hip or knee PJIs including P. acnes diagnosed from August-2002 to July-2013 in two university hospitals were included...
March 8, 2017: Anaerobe
https://www.readbyqxmd.com/read/28286023/peritoneal-infection-due-to-peptoniphilus-harei-in-a-patient-with-intestinal-occlusion
#9
Fernando Cobo, Javier Rodríguez-Granger, Antonio Sampedro, José María Navarro-Marí
Peptoniphilus harei is a Gram-positive anaerobic coccus mainly involved in polymicrobial infections. We report a case of peritoneal infection in a patient with intestinal occlusion. A 48-year-old woman presented with ascitis after an occlusive syndrome. Culture of peritoneal fluid resulted on P. harei isolation. Treatment was performed and resolution of this infection was documented.
March 7, 2017: Anaerobe
https://www.readbyqxmd.com/read/28286022/first-report-of-human-infection-by-christensenella-minuta-a-gram-negative-strickly-anaerobic-rod-that-inhabits-the-human-intestine
#10
Beatriz Lopez Alonso, Alvaro Irigoyen von Sierakowski, Juan Antonio Sáez Nieto, Antonio Beltran Rosel
Christensenella minuta is a Gram-negative strictly anaerobic short rod that inhabits the human gut. This bacterium was isolated in a mixed infection with Desulfovibrio desulfuricansfrom the blood of a patient with a diagnosis of acute appendicitis. The strain was identified by 16S rRNA sequence analysis. As far as we know, this is the first time C.minuta has been isolated from a human clinical specimen.
March 7, 2017: Anaerobe
https://www.readbyqxmd.com/read/28279858/characterization-of-isolates-of-eisenbergiella-tayi-a-strictly-anaerobic-gram-stain-variable-bacillus-recovered-from-human-clinical-materials-in-canada
#11
Kathryn Bernard, Tamara Burdz, Deborah Wiebe, Brittany M Balcewich, Tina Zimmerman, Philippe Lagacé-Wiens, Linda M N Hoang, Anne-Marie Bernier
Eisenbergiella gen. nov. was proposed in 2014 to describe an obligate anaerobic, structurally Gram-positive but Gram-stain-negative-appearing bacillus recovered from the blood culture of an elderly Israeli man. Here, we describe features for eight blood culture isolates as well one appendix-derived isolate, recovered from seven patients located in two Canadian provinces, which by 16S rRNA gene sequencing, were identifiable as Eisenbergiella tayi, the sole validly- named species in this genus. After whole genome sequencing, isolates were found to be essentially identical (96...
March 7, 2017: Anaerobe
https://www.readbyqxmd.com/read/28279860/inhibition-of-spore-germination-growth-and-toxin-activity-of-clinically-relevant-c-%C3%A2-difficile-strains-by-gut-microbiota-derived-secondary-bile-acids
#12
Rajani Thanissery, Jenessa A Winston, Casey M Theriot
The changing epidemiology of Clostridium difficile infection over the past decades presents a significant challenge in the management of C. difficile associated diseases. The gastrointestinal tract microbiota provides colonization resistance against C. difficile, and growing evidence suggests that gut microbial derived secondary bile acids (SBAs) play a role. We hypothesized that the C. difficile life cycle; spore germination and outgrowth, growth, and toxin production, of strains that vary by age and ribotype will differ in their sensitivity to SBAs...
March 6, 2017: Anaerobe
https://www.readbyqxmd.com/read/28279859/factors-associated-with-clostridium-difficile-infection-a-nested-case-control-study-in-a-three-year-prospective-cohort
#13
Nagham Khanafer, Philippe Vanhems, Frédéric Barbut, Christine Luxemburger
BACKGROUND: Clostridium difficile infection (CDI) is a serious medical condition that is associated with substantial morbidity and mortality. Identification of risk factors associated with CDI and prompt recognition of patients at risk is key to successfully preventing CDI. METHODS: A 3-year prospective, observational, cohort study was conducted in a French university hospital and a nested case-control study was performed to identify risk factors for CDI. Inpatients aged 18 years or older, suffering from diarrhea suspected to be related to CDI, were asked to participate...
March 6, 2017: Anaerobe
https://www.readbyqxmd.com/read/28279857/performance-of-two-blood-culture-systems-to-detect-anaerobic-bacteria-is-there-any-difference
#14
Manica Mueller-Premru, Samo Jeverica, Lea Papst, Elisabeth Nagy
We studied the performance characteristics of two blood culture (BC) bottles/systems, (i) BacT/ALERT-FN Plus/3D (bioMérieux, Marcy l'Étoile, France) and (ii) BACTEC-Lytic/9000 (Becton Dickinson, Sparks, USA) for detection of growth and time-to-positivity (TTP) against a balanced and diverse collection of anaerobic bacterial strains (n = 48) that included reference strains (n = 19) and clinical isolates (n = 29) of 32 species (15 Gram-negative and 17 Gram-positive) was tested. Standard suspension of bacteria was inoculated to each bottle in duplicates and incubated in the corresponding system...
March 6, 2017: Anaerobe
https://www.readbyqxmd.com/read/28249795/early-exposure-to-agricultural-soil-accelerates-the-maturation-of-the-early-life-pig-gut-microbiota
#15
Nguyen Vo, Tsung Cheng Tsai, Charles Maxwell, Franck Carbonero
Reduced microbial exposure in early childhood is postulated to be associated with subsequent immune deficiencies and associated health conditions. This corollary to the "hygiene hypothesis" has grown of popularity in the medical field, but can only be really tested with animal models. Based on previous observation that access to outdoor environment improves piglets' growth performance, we simulated early microbial exposure by providing pigs with topsoil during the lactation phase. Specifically, pigs from 20 litters were assigned to either control treatments (C) or soil treatments (S): pigs exposed to topsoil from day 4 postpartum to the end of lactation...
February 27, 2017: Anaerobe
https://www.readbyqxmd.com/read/28246035/functional-gene-profiling-through-metarnaseq-approach-reveals-diet-dependent-variation-in-rumen-microbiota-of-buffalo-bubalus-bubalis
#16
Ankit T Hinsu, Nidhi R Parmar, Neelam M Nathani, Ramesh J Pandit, Anand B Patel, Amrutlal K Patel, Chaitanya G Joshi
Recent advances in next generation sequencing technology have enabled analysis of complex microbial community from genome to transcriptome level. In the present study, metatranscriptomic approach was applied to elucidate functionally active bacteria and their biological processes in rumen of buffalo (Bubalus bubalis) adapted to different dietary treatments. Buffaloes were adapted to a diet containing 50:50, 75:25 and 100:0 forage to concentrate ratio, each for 6 weeks, before ruminal content sample collection...
February 27, 2017: Anaerobe
https://www.readbyqxmd.com/read/28242337/survival-of-vaginal-microorganisms-in-three-commercially-available-transport-systems
#17
Allison L DeMarco, Lorna K Rabe, Michele N Austin, Kevin A Stoner, Hilary A Avolia, Leslie A Meyn, Sharon L Hillier
Transport systems are used to collect and maintain the viability of microorganisms. Two Amies media based transport systems, BD CultureSwab™ MaxV(+) Amies Medium without Charcoal (MaxV(+)) and Fisherfinest(®) with Amies gel Transport Medium without charcoal (Fisherfinest(®)) were compared to a Cary-Blair media based transport system, Starswab(®) Anaerobic Transport System (Starswab(®)), for their capacity to maintain the viability of 17 clinical microorganisms commonly isolated from the vagina (Lactobacillus crispatus, L...
February 24, 2017: Anaerobe
https://www.readbyqxmd.com/read/28238846/first-report-on-sepsis-caused-by-porphyromonas-pogonae
#18
Percy Schröttner, Katharina Heidrich, Wolfram W Rudolph, Friedrich Stölzel, Enno Jacobs, Florian Gunzer
We report on a 62 year old patient who developed sepsis due to an infection caused by Porphyromonas pogonae, a recently described species of the bacterial genus Porphyromonas. This is the first case of an invasive infection with this pathogen.
February 24, 2017: Anaerobe
https://www.readbyqxmd.com/read/28238845/multilocus-sequence-typing-analyses-of-clostridium-perfringens-type-a-strains-harboring-tpel-and-netb-genes
#19
V Nakano, A Ignacio, L Llanco, V Bueris, M P Sircili, M J Avila-Campos
Clostridium perfringens is an anaerobic bacterium ubiquitous in various environments, especially in soil and the gastrointestinal tract of healthy humans and animals. In this study, multilocus sequence typing protocol was used to investigate genotypic relationships among 40 C. perfringens strains isolated from humans and broiler chicken with necrotic enteritis [NE]. The results indicated a few clonal populations, mainly observed in human strains, with 32.5% of all strains associated with one of three clonal complexes and 30 sequences types...
February 24, 2017: Anaerobe
https://www.readbyqxmd.com/read/28238844/bacterial-species-colonizing-the-vagina-of-healthy-women-are-not-associated-with-race
#20
May A Beamer, Michele N Austin, Hilary A Avolia, Leslie A Meyn, Katherine E Bunge, Sharon L Hillier
The vaginal microbiota of 36 white versus 25 black asymptomatic women were compared using both cultivation-dependent and -independent identification. Significant differences by race were found in colonization and density of bacterial species. However, exclusion of 12 women with bacterial vaginosis by Nugent criteria resulted in no significant differences by race.
February 24, 2017: Anaerobe
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