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Protein and Peptide Letters

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https://www.readbyqxmd.com/read/30406735/purification-and-characterization-of-thermostable-cellobiohydrolase-from-thermotoga-petrophila
#1
Muhammad Nauman Aftab, Ikram Ul Haq, Syed Fahid Tahir, Fatima Akram, Ali Nawaz, Hamid Mukhtar
BACKGROUND: Cellulose, being the most abundant biopolymer found in nature, can be utilized for bioethanol production to cater the future energy needs. Due to increased usage of fossil fuel it has been predicted that fossil fuel reserves may be depleted by year 2050. These concerns need serious attention and focus should be diverted to renewable fuels that are based on natural resources. Cellulases including exoglucanase (cellobiohydrolases) are the key enzymes that are produced by cellulolytic micro-organisms for the biodegradation of natural resource (cellulose) into fermentable reducing sugars...
November 7, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/30381058/anti-infective-peptides-to-enhance-the-host-innate-response-design-development-and-delivery
#2
Raymond S Norton
BACKGROUND: Inducible nitric oxide synthase (iNOS or NOS2) produces nitric oxide (NO) and related reactive nitrogen species, which are critical effectors of the host innate response and play key roles in the intracellular killing of bacterial and parasitic pathogens. The SPRY domain-containing SOCS box proteins SPSB1 and SPSB2 are key physiological regulators of this important enzyme. Disrupting the endogenous SPSB-iNOS interaction should prolong the intracellular lifetime of iNOS and enhance the production of NO, and therefore be beneficial in treating chronic and persistent infections such as tuberculosis...
October 31, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/30381057/breakthroughs-in-venom-peptide-screening-methods-to-advance-future-drug-discovery
#3
Tanya Napolitano, Mande Holford
BACKGROUND: Venom peptides are a proven resource for identifying novel drugs, however the process of identifying bioactive venom peptides is labor intensive, costly, and rarely results in pharmaceutical success. As venom peptides are modulators of ion channels and receptors their potential for manipulating cell signals in diseased states are unique and offer an untapped resource for finding new medicines. Recent advances in -omic technologies, and microfluidic biosensing systems have transformed how venom peptides are discovered and characterized...
October 31, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/30381056/esculentin-1a-derived-antipseudomonal-peptides-limited-induction-of-resistance-and-synergy-with-aztreonam
#4
Bruno Casciaro, Maria Rosa Loffredo, Vincenzo Luca, Walter Verrusio, Mauro Cacciafesta, Maria Luisa Mangoni
BACKGROUND: The massive use of antibiotics has led to the selection of resistant bacterial strains that are difficult to eradicate. Among these, Pseudomonas aeruginosa most frequently colonizes and infects the airways of cystic fibrosis patients. Cationic Antimicrobial Peptides (AMPs) represent interesting molecules for the development of new antimicrobial agents. Thanks to their mechanism of action that involves the permeabilization of the bacterial cytoplasmic membrane, the induction of resistance is quite limited...
October 31, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/30381055/peptidomimetics-targeting-protein-protein-interactions-for-therapeutic-development
#5
Gan Zhang, Jessica Andersen, Guillermo Gerona-Navarro
Interactions between proteins play a key role in nearly all cellular process, and therefore, disruption of such interactions may lead to many different types of cellular dysfunctions. Hence, pathologic protein-protein interactions (PPIs) constitute highly attractive drug targets and hold great potential for developing novel therapeutic agents for the treatment of incurable human diseases. Unfortunately, the identification of PPI inhibitors is an extremely challenging task, since traditionally used small molecule ligands are mostly unable to cover and anchor on the extensive flat surfaces that define those binary protein complexes...
October 31, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/30381054/stealth-peptides-target-cellular-powerhouses-to-fight-rare-and-common-age-related-diseases
#6
Hazel H Szeto
Mitochondria are the primary source of energy in most tissues and are particularly important for tissues with high energy demand such as skeletal muscle, heart, brain and retina. Mitochondrial dysfunction results in cellular energy deficiency, triggers the production of reactive oxygen species, and initiates various cell death and inflammatory pathways. This review describes a family of mitochondria-targeted tetrapeptides (SS peptides) that have been shown to improve mitochondrial bioenergetics, reduce mitochondrial oxidative stress, and prevent cell death and inflammation...
October 31, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/30381053/structurally-constrained-insulin-analogs-by-directed-stepwise-cross-linking
#7
Fa Zhang, John Mayer, Vasily Gelfanov, Fa Liu, Richard DiMarchi
BACKGROUND: Research has been directed at the optimization of insulin for medicinal purposes. An insulin analog that could be reversibly activated might provide more precise pharmacokinetic control and broaden the inherent therapeutic index of the hormone. The prospect of using intramolecular structural constraint to reversibly inactive insulin might constitute the first step to achieving such an optimized analog. Chemically crosslinked insulin analogs have been reported where two amines are covalently linked by reaction with symmetrical bifunctional active esters...
October 31, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/30381052/the-synthesis-of-sulfated-ccr5-peptide-surrogates-and-their-use-to-study-receptor-ligand-interactions
#8
Fred Naider, Jacob Anglister
BACKGROUND: Tyrosine sulfation is an important post-translational modification of secreted and membrane proteins in multi-cellular organisms. This modification is catalyzed by tyrosylprotein sulfotransferases that often modify tyrosine residues in their target substrates in a heterogeneous manner. Chemokine receptors such as CCR5, which play roles in inflammation, immunity and viral infection, are sulfated on tyrosine residues in their extracellular N-termini. The heterogeneity of the sulfation has made it difficult to obtain atomic-resolution information on this region of CCR5...
October 31, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/30370842/the-unique-inhibitory-igg-receptor-fc%C3%AE-riib
#9
Zhenzhen Xu, Yanfen Zhang, Zhongcheng Liu, Lipeng Liu, Zhe Cui, Kewei Bi, Nan Zhang
BACKGROUND: FcγRIIb is the only inhibitory IgG receptor, which is divided into three subtypes of FcγRIIb1, FcγRIIb2 and FcγRIIb3. It is mainly responsible for the immune balance in vivo by cross-linking with the activated receptor to intracellular transduction inhibitory signals,and it plays an important biological role in the negative regulation of innate immunity and adaptive immunity. An abnormal expression of FcγRIIb on cells would result in autoimmune diseases, infectious diseases and so forth...
October 26, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/30370841/antimicrobial-peptides-recent-insights-on-biotechnological-interventions-and-future-perspectives
#10
Rajeshwari Sinha, Pratyoosh Shukla
With the unprecedented rise of drug-resistant pathogens, particularly antibiotic-resistant bacteria, and no new antibiotics in the pipeline over the last three decades, the issue of antimicrobial resistance has emerged as a critical public health threat. Antimicrobial peptides have garnered interest as a viable solution to this grave issue and are being explored for their potential antimicrobial applications. Given their low bioavailability in nature, tailoring new AMPs or strategizing approaches for increasing the yield of AMPs, therefore, becomes pertinent...
October 26, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/30345912/identification-of-conotoxins-with-novel-odd-number-of-cysteine-residues-from-the-venom-of-a-marine-predatory-gastropod-conus-leopardus-found-in-andaman-sea
#11
R P Rajesh, Benjamin Franklin Jayaseelan
Conotoxins are neuro-pharmacologically active cysteine rich peptides isolated from the venom complex of marine cone snails. These are usually made of even number of cysteines. In this study we characterised six novel conotoxin sequences from the venom of Conus leopardus collected from the Andaman Sea, namely Le907 [C-C], Le868 [C-C], Le933 [-C-CC], Le949 [-C-CC], Le1988 [C-C-CC-C] and Le1642 [CC-C-C] using de novo mass spectrometry-based sequencing methods. Astonishingly 3 of these peptides possess novel arrangements of cysteine residues with odd number of cysteines [-C-CC; C-C-CC-C], namely Le933, Le949 and Le1988...
October 22, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/30338728/refolding-and-activation-from-bacterial-inclusion-bodies-of-trypsin-i-from-sardine-sardinops-sagax-caerulea
#12
Manuel I Carretas-Valdez, Francisco J Cinco-Moroyoqui, Marina J Ezquerra-Brauer, Enrique Marquez-Rios, Idania E Quintero-Reyes, Alonso A Lopez-Zavala, Aldo Alejandro Arvizu-Flores
Trypsin from fish species is considered as a cold-adapted enzyme that may found potential biotechnological applications. In this work, the recombinant expression, refolding and activation of trypsin I (TryI) from Monterey sardine (Sardinops sagax caerulea) are reported. TryI was overexpressed in Escherichia coli BL21 as a fusion protein of trypsinogen with thioredoxin. Refolding of trypsinogen I was achieved by dialysis from bacterial inclusion bodies with a recovery of 16.32 mg per liter of Luria broth medium...
October 19, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/30338727/effect-of-acyl-homoserine-lactone-on-recombinant-production-of-human-insulin-like-growth-factor-1-in-batch-culture-of-escherichia-coli
#13
Valiollah Babaeipour, Hossein Vahidi, Shamila Alikhani, Javad Ranjbari, Abbas Alibakhshi, Maryam Tabarzad
IGF-I as a human growth factor produced in Escherichia Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.6 kDa. Up to now, E. coli expression system has been widely used as a host to produce rhIGF-1 with high yields. Acyl homoserine lactones (AHLs) are intercellular signaling molecules used in quorum sensing by Gram-negative bacteria. Quorum sensing is a cell density-dependent gene regulation process that allows bacterial cells to express specific genes only when signaling molecules reach a sufficient concentration...
October 19, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/30289060/efficient-expression-and-purification-of-cryptochrome1-from-columbia-livia-in-e-coli
#14
Xiaoxia Yuan, Wenhao Wang, Xuefeng Wang, Wenjian Wu, Jing Wang
Cryptochrome is a flavin-binding blue-light photoreceptor that functions in growth and development in plants, the circadian clock in animals and navigation in birds. However, a lack of purified cryptochrome has hindered studies of the structure and function of this protein. In this study, we obtained a substantial amount of the Columbia livia Cryptochrome1 (ClCry1) protein by using a prokaryotic expression system. In addition, we performed comprehensive experiments to assess the influence of several factors on the purification and yield of ClCry1, such as the inducer that was used, temperature, duration of expression and type of growth medium...
October 4, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/30289063/structural-insights-on-the-obscurin-binding-domains-in-titin
#15
Nathan T Wright, Allyn G Letourneau
INTRODUCTION: The giant muscular proteins titin and obscurin bind to each other at the Z-disk during muscle development. This binding event is mediated through two domains from each protein: ZIg9/10 from titin and Ig58/59 from obscurin. This interaction helps stabilize and organize the sarcomere; ablation of this binding leads to muscular dystrophy. OBJECTIVE: Here we solve the high-resolution solution structure of titin ZIg10 and further delineate which sections of titin bind to obscurin...
October 3, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/30289062/qrpr-and-hcqrpq-two-peptides-from-soybean-have-an-inhibitory-effect-on-the-proliferation-of-hepg2-cells
#16
Fengguang Pan, Lin Wang, Na Guo, Mingdi Zhang, Heqin Xing
In this study, we investigated the possible inhibitory effect of soy peptides QRPR and HCQRPQ on human liver cancer cells (HepG2) and their underlying mechanisms. An MTS assay showed that QRPR used in combination with HCQRPQ had a significant inhibitory effect on the proliferation of HepG2 cells compared to the effect of QRPR or HCQRPQ alone, and the cytotoxicity was low. An Annexin V/PI assay revealed that QRPR used in combination with HCQRPQ induced apoptosis in HepG2 cells; this outcome was also confirmed by the increased expression of caspase-3...
October 3, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/30289061/baltpla2-a-new-phospholipase-a2-from-bothrops-alternatus-snake-venom-with-antiplatelet-aggregation-activity
#17
Edigar Henrique Vaz Dias, Tamires Dos Santos Paschoal, Alisson Pereira da Silva, Deborah Fernanda da Cunha Pereira, Bruna Barbosa de Sousa Simamoto, Mariana Santos Matias, Fernanda Maria Santiago, Jose Cesar Rosa, Andreimar Soares, Norival A Santos-Filho, Fabio de Oliveira, Carla Cristine Neves Mamede
In last decades, snake venoms have aroused great interest of the medicine due to the pathophysiological effects caused by their toxins. These include the phospholipases A2, low molecular weight proteins capable of causing haemorrhagic, myotoxic, inflammatory and neurotoxic effects after an ophidian accident. The present work describes the isolation and biochemical characterization of a new PLA2 isolated from the B. alternatus snake venom, which was named BaltPLA2. The rapid and efficient purification of this toxin was performed using only two chromatography steps (anion exchange and hydrophobic chromatography)...
October 3, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/30255743/serum-proteome-profiling-to-identify-proteins-promoting-pathogenesis-of-non-atopic-asthma
#18
Samina Ejaz, Faiz Ul-Hassan Nasim, Muhammad Ashraf, Sami Ahmad
BACKGROUND: Asthma is the inflammatory disorder of airways highly prevalent in both, children and adults all over the world. The aim of this study was to investigate the serum proteome for the identification of proteins contributing to the pathogenesis of non-atopic asthma. METHODS: Protein expression profiling of sera from non-atopic asthmatic patients (n=73) and controls (n=99) was carried out using 1D SDS PAGE. Differentially expressed protein bands were compared with controls, cut from the gel and identified by LC-Q-TOF_MSMS analysis...
September 25, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/30255742/recent-developments-toward-antibody-engineering-and-affinity-maturation
#19
Abhishek Singh Rathore, Animesh Sarker, Rinkoo Devi Gupta
Monoclonal antibodies have been proven to deliver significant contribution in health industry for the development of both therapeutics and diagnostics. Efforts have been made to achieve immunoglobulin with high antigen specificity and stability. In this regard, smaller fragment of antibody has been constructed as an alternative of full immunoglobulin molecules due to the feasibility of recombinant production in various host cells. Antibody fragments are that part of an immunoglobulin which can form a complete epitope binding site and also retain the binding efficiency and accuracy of a whole antibody...
September 25, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/30255741/peptide-based-anxiolytics-the-molecular-aspects-of-heptapeptide-selank-biological-activity
#20
Tatiana V Vyunova, Lioudmila Andreeva, Konstantin Shevchenko, Nikolay Myasoedov
Anxiety and mood disorders are the most abundant mental health problems worldwide. The commonly used in clinical practice anxiolytics are focused on pharmacological modulation of brain GABA system activity. As a rule, their use presents a wide spectrum of clinical issues such as dependence, memory impairment and etc. There is an increasing appreciation of the role of neuropeptides and bioactive lipids in the pathophysiology of mood and anxiety disorders as "mild" agents. Heptapeptide Selank (Thr-Lys-Pro-Arg-Pro-Gly-Pro) exhibits prolonged anti-anxiety and nootropic effects...
September 25, 2018: Protein and Peptide Letters
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