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Protein and Peptide Letters

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https://www.readbyqxmd.com/read/28294044/ca2-binding-and-conformational-switch-of-the-photoprotein-mnemiopsin
#1
Shima Tarahomi, Reza H Sajedi, Hossein Rahmani, Bijan Ranjbar, Majid Taghdir
Bioluminescence in Ca2+-binding photoproteins is an intramolecular reaction triggered by the addition of Ca2+. A comparative study has been done on Ca2+-depleted and Ca2+-loaded apo-mnemiopsin to understand the structural transition of the photoprotein by Ca2+ binding. Ca2+ is removed by TCA (trichloroacetic acid) precipitation to obtain Ca2+-depleted apo-mnemiopsin. UV-visible, CD and fluorescence spectroscopic studies demonstrate that the addition of Ca2+ is brought about by the overall structure of apo-mnemiopsin becomes more open in a concentration-dependent manner without significantly influencing the secondary structure and indicate that the Ca2+-depleted form of apo-mnemiopsin, in contrast to most other EF-hand calcium binding proteins, adopt a closed conformation when compared to the Ca2+-loaded form...
March 10, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28266265/effects-of-dietary-iron-modulation-on-gut-microbial-composition-and-function-in-monogastrics-a-review
#2
Guixin Qin, Rui Han, Bao Zhao, Hailong Jiang, Mohammed Hamdy Farouk, Chun Zhang, Dongsheng Che
Dietary iron is a crucial nutrient element for biological processes of both hosts and gut microbiota. Deficiency in dietary iron is a highly common disorder in the developing locations of the world and can be healed by oral iron administration or complementary iron diet. While the redundant iron that enters the gut lumen leads to negative effects, and modulates the gut microbial composition and function. Such modulation led to a significant effect on vital biological pathways of the host, including metabolic disease (obesity and type 2 diabetes), metabolites (SCFA, blood glucose and cholesterol), bile acid metabolism, endocrine, neural, and other well-being patterns...
March 5, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28260503/in-vivo-neutralization-of-botulinum-neurotoxin-serotype-e-using-rabbit-polyclonal-antibody-developed-against-bont-e-light-chain
#3
Sarita Sarita, Ponmariappan Sarkaraisamy, Arti Sharma, D V Kamboj, A K Jain
Clostridium botulinum is an obligate anaerobic, Gram positive bacterium that secretes extremely toxic substances known as botulinum neurotoxins (BoNTs) that cause serious paralytic illness called botulism. Based upon the serological properties, these neurotoxin have been classified into seven serotypes designated from A to G. Due to extreme toxicity of BoNTs, these neurotoxins have been designated as category A biowarfare agents. There is no commercial neutralizing antibody available for the treatment of botulism...
March 1, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28260502/selective-binding-baff-april-by-the-in-and-outside-conservative-region-of-bcma
#4
Chang Zheng, Xiaojuan Zhang, Zhen Zhao, Xiaofei Hao, Jing Wei, Jian Sun
B-cell activating factor (BAFF) and a proliferation-inducing ligand (APRIL) play vital roles in the pathogenesis of autoimmune diseases. B cell maturation antigen (BCMA), a receptor of both BAFF and APRIL shows higher affinity for APRIL than for BAFF. In this study, the conserved region of BCMA was divided into two segments BCMA1 (NEYFDSLLHACIPC) and BCMA2 (QLRCSSNTPPLT) with the aim to investigate their contribution to the ligands binding, BCMA1 contains the conservative DxL domain while BCMA2 is the segment outside the main functional area...
March 1, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28245756/diosmetin-induces-cell-apoptosis-by-regulating-cyp1a1-cyp1a2-due-to-p53-activation-in-hepg2-cells
#5
Bin Liu, Kaiqiao Jia, Yu Yang, Siyuan Hao, Caijie Lu, Fengming Xu, Dehui Feng, Runzhi Zhu
It was explored that CYP1 family of cytochromes P450 were over-expressed in several types of cancer. Our study aimed to characterize anti-proliferative activity and metabolism of the natural flavonoid diosmetin in the human hepatoma cell HepG2, expressing CYP1 family. Dios-induced cell apoptosis could be reversed due to p53 blockade and the cellular P53 and CYP1A1/CYP1A2 proteins levels were examined. P53 and CYP1A1/CYP1A2 proteins were up-regulated by Dios; when PFT-α was added into cells, the P53 levels were down-regulated accompanied with up-regulated CYP1A1/CYP1A2...
February 27, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28245755/towards-binding-mechanism-of-cu2-on-creatine-kinase-from-pelodiscus-sinensis-molecular-dynamics-simulation-integrating-inhibition-kinetics-study
#6
Yan Cai, Jinhyuk Lee, Wei Wang, Yong-Doo Park, Guo-Ying Qian
Cu2+ is well known to play important roles in living organisms having bifacial distinction: essential microelement that is necessary for a wide range of metabolic processes but hyper-accumulation of Cu2+ can be toxic. The physiological function of Cu2+ in ectothermic animals such as Pelodiscus sinensis (Chinese soft-shelled turtle) has not been elucidated. In this study, we elucidated effect of Cu2+ on the energy producing metabolic enzyme creatine kinase (CK), which might directly affect energy metabolism and homeostasis of P...
February 27, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28240162/effects-of-dietary-non-fiber-carbohydrates-on-composition-and-function-of-gut-microbiome-in-monogastrics-a-review
#7
Hailong Jiang, Dongsheng Che, Guixin Qin, Xiangjie Kong, Mohammed Hamdy Farouk
Non-fiber carbohydrates (NFC) have a crucial function on the gut health of monogastrics. This paper aims to review the relevant published materials on the influence of NFC's on the gut's microbial population and composition in monogastrics, and points out the areas of the required research. Total bacteria count and Lactobacillus sp. were decreased with an increase in composition of dietary NFC intake, as well as accompanied by a decrease in the short-chain fatty acids (SCFAs) levels. Consequently, some metabolites were affected by the accumulation of the bile acids, including molecules which control different gene expression levels, as regulators involved in glucose (FXR and TGR5) and fat metabolism (cholesterol)...
February 23, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28240161/dihydromyricetin-reduces-tgf-%C3%AE-via-p53-activation-dependent-mechanism-in-hepatocellular-carcinoma-hepg2-cells
#8
Xiaojie Huang, Tianming Lian, Xiaoqian Guan, Bin Liu, Zeqing Song, Jingjing Zhang, Shiting Bao, Runzhi Zhu
Natural antineoplastic drug development is crucial to treatment of clinical oncology. Dihydromyricetin, a bioactive flavonoid compound was extracted from the stems and leaves of Ampelopsis grossedentata. It exhibited anticancer activity and induced apoptosis in human hepatocellular carcinoma cells according to our previous studies. In this study, we demonstrated that DHM could significantly inhibit proliferation and induce apoptosis in HepG2 cells with MTT and Flow Cytometry methods. It is very interesting that we found DHM could regulate TGF-β signal pathway and which has a crosstalk with P53, Smad3 and P-Smad2/3 proteins...
February 23, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28240160/dietary-additive-probiotics-modulation-of-the-intestinal-microbiota
#9
Shenglan Hu, Li Wang, Zongyong Jiang
The importance of the intestinal microbiota of animals is widely acknowledged because of its vital role in the health of animals. There are complex communities of microbiota, which colonize the gastrointestinal tract. Intestinal microbiota are conductive to animal health and the development of the host immune system. Probiotics are commonly used dietary additives where they provide the host with many beneficial functions, such as modulating intestinal homeostasis and promoting gut health. These beneficial effects of probiotics may accrue from the inhibiting the growth of pathogenic bacteria and promoting the growth of beneficial flora in the gastrointestinal tract...
February 23, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28240159/supplementation-of-milky-flavors-improves-the-reproductive-performance-and-gut-function-using-sow-model
#10
Long He, Jianjun Zang, Ping Liu, Peixin Fan, Peixia Song, Jingshu Chen, Yongxi Ma, Wei Ding, Xi Ma
This study was conducted to evaluate the effect of flavors on reproductive performance of sows and we also studied its effect on gut barrier function. Forty-eight Landrace × Yarkshire sows were randomly allotted and fed a basal diet added 0%, 0.05% or 0.10% flavor feed, respectively from parturition to day 28 of weaning. The results showed that supplementation of 0.05% or 0.10% flavors increased average daily feed intake (ADFI) of sows and average daily gain (ADG) of piglets, decreased the weight losses of sows, increased the survival ratio of weaning piglets (P < 0...
February 23, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28240158/biodiesel-production-from-citrillus-colocynthis-oil-using-based-catalytic-reaction-and-characterization-studies
#11
Imededdine A Nehdi, Hassen M Sbihi, Lahssen El Blidi, Umer Rashid, Saud I Al-Resayes
In this investigation, an enzyme catalyzed process was optimized to prepare methyl esters from crude Citrullus colocynthis oil (CCO). Novozym 435 was used to synthesize biodiesel by transesterification of CCO with methanol. Use of tert-butanol as a reaction medium eliminated negative effects caused by excess methanol and formation of glycerol byproduct. Several variables affecting the Citrullus colocynthis methyl ester (CCME) yield including lipase quantity, water content, methanol/oil mole ratio, reaction temperature, reaction medium composition, and reaction time were optimized...
February 23, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28240165/diosmetin-a-potential-p53-activator-performs-anticancer-effect-by-regulating-cell-cycling-and-cell-proliferation-in-hepg2-cells
#12
Bin Liu, Miaomiao Zhang, Shuna Liu, Ying Jie, Jingjing Zhang, Weiqiang Zheng, Kurihara Hiroshi, Runzhi Zhu
Diosmetin (Dios), a flavone aglycone, is a major active ingredient of many herbal medicines. Previous studies implicated that the potential antitumor activities of Dios are mediated through multiple cell signaling pathways, however more investigations are required to reveal the targets and mechanisms of Dios. In this study, our results demonstrated that cell cycle progression and proliferation of HepG2 cells were inhibited by Dios. Meanwhile, Dios-induced liver cancer cell apoptosis was related with the p53 activation...
February 22, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28240164/effects-of-dietary-mycotoxins-on-gut-microbiome
#13
Kexin Du, C Wang, Ping Liu, Yuan Li, Xi Ma
The harmful effects of mycotoxins on intestinal health have received worldwide attention. Mycotoxins are toxic secondary metabolites produced by filamentous fungi, and include aflatoxins, ochratoxins, patulin, fumonisins, zearalenone, trichothecenes, and ergot alkaloids. Insuring the absence or low levels of mycotoxins is critical for food and feed safety. Currently, the studies in this field have illuminated the adverse effects of mycotoxins on gut health including intestinal integrity and the gut-associated immune system...
February 22, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28240163/effects-of-environmental-temperature-and-dietary-fat-content-on-the-performance-and-heat-production-and-substrate-oxidation-in-growing-pigs
#14
Rui Han, Hailong Jiang, Dongsheng Che, Nan Bao, Dong Xiang, Feifei Liu, Huaming Yang, Zhibin Ban, Guixin Qin
This study aimed to evaluate the effect of temperature and dietary fat level on growth performance, heat production, nutrient oxidation and nitrogen balance in growing pigs. Thirty-two pigs (Duroc × Landrace × Large White) with initial weight of 25±1.91 kg were assigned to treatments in 2×4 factorial design. All pigs fed with two isoenergetic and isoproteic diets of different fat levels (low fat level: 3.68% fat of dry matter (DM) and high fat level: 8.39% fat of DM) under four environmental temperatures (23, 18, 13 and 8 ºC)...
February 22, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28219317/dietary-fiber-gap-and-host-gut-microbiota
#15
Meng Han, Ping Liu, Defa Li, Yuan Li, Xi Ma
Accumulating evidence are dramatically increasing access to the facts that the gut microbiota plays a pivotal role in host metabolism and health, which revealed the possibility of a plethora of associations between gut bacteria and human diseases. Several functional roles carried out by a major class of host's diet, such as fiber. Fiber is the main source of microbiota-accessible carbohydrate in the diet of humans. In modern diet, it is difficult to intake dietary fiber as enough as recommended standard. The low-fiber diet in the modern life, known as fiber gap, can trigger to a substantial depletion of the human gut microbiota diversity and beneficial metabolites...
February 20, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28219316/from-disorder-to-mis-order-structural-aspects-of-pathogenic-oligomerization-in-conformational-diseases
#16
Chin Pang Benny Yiu, Yu Wai Chen
Proteins implicated in neurological conformational diseases contain substantial amounts of "intrinsic disorder". These native monomeric functional states may transit into some oligomeric states that have high β-sheet contents and seed the formation of insoluble amyloid fibrils. The prevailing view is that these "toxic" oligomers should be targeted for drug development. Here, an overview of the diseases was presented, within the general framework of the oligomerization of intrinsically disordered proteins. These systems pose some specific challenges to structural studies: the toxic oligomers are transient, low in concentration, and often need to be studied in a heterogeneous environment...
February 20, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28201964/plasmodium-falciparum-heat-shock-protein-70-lacks-immune-modulatory-activity
#17
Ofentse Jacob Pooe, Gabriele Köllisch, Holger Heine, Addmore Shonhai
Heat shock protein 70 (Hsp70) family are conserved molecules that constitute a major part of the cell's protein folding machinery. The role of Hsp70s of parasitic origin in host cell immune modulation has remained contentious. This is largely due to the fact that several studies implicating Hsp70 in immune modulation rely on the use of recombinant protein derived from bacteria which is often fraught contamination. Thus, in the current study, we expressed recombinant Plasmodium falciparum Hsp70 (PfHsp70) using in three bacterial expression hosts: E...
February 14, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28201963/inhibition-of-amyloid-like-fibril-formation-of-trypsin-by-red-wines
#18
Márta Kotormán, Phanindra Babu Kasi, László Halász, Attila Borics
The aim of the present study was to examine the potential role and applicability of dietary supplements in reducing the risk of development of amyloid diseases associated with the gastrointestinal tract, such as type II diabetes. Trypsin, a well-known serine protease was used as a model protein in our experiments. The effect of various red wines on the formation of amyloid-like fibrils of trypsin was studied in vitro, in aqueous ethanol, at pH 7.0. Turbidity measurements, aggregation kinetics experiments, Congo red binding assays and electronic circular dichroism spectroscopic measurements were used to follow the aggregation process in the presence or absence of various red wines...
February 14, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28190398/transforming-cytosolic-proteins-into-insoluble-and-membrane-toxic-forms-by-genetic-pathological-or-environmental-factors
#19
Jianxing Song
An increasing spectrum of diseases other than neurodegenerative diseases is characteristic of aggregation of specific proteins, while aggregation of a large number of non-specific proteins are associated with aging down to Escherichia coli. Triggered by disease-causing mutations and aging-associated damages, many well-folded cytosolic proteins become "completely insoluble" in vivo. As facilitated by our discovery in 2005 that "completely insoluble" proteins could be solubilized in unsalted water, we have deciphered that disease- and aging-associated factors act to eliminate the native folds of human VAPB-MSP and SOD1, as well as E...
February 9, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28190397/when-good-goes-awry-the-aggregation-of-protein-therapeutics
#20
Jennifer M Redington, Leonid Breydo, Vladimir N Uversky
Protein therapeutics are playing an increasingly important role in treatment of a variety of human diseases. However, like the rest of proteins, they are susceptible to aggregation. Aggregation of proteinaceous pharmaceuticals can cause a loss of efficacy and, potentially, cytotoxicity and an immunogenic response. This review describes various ways protein therapeutics aggregate and a variety of approaches taken to prevent or minimize this process.
February 9, 2017: Protein and Peptide Letters
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