Read by QxMD icon Read

Protein and Peptide Letters

Luisa Zupin, Katia Rupel, Giulia Ottaviani, Augusto Poropat, Roberto Di Lenarda, Matteo Biasotto, Sergio Crovella
Laser therapy (LT) has been employed for treatment of oral mucositis (OM)due to its anti-inflammatory, analgesic and bio-stimulatory effects. This pilot study investigated the effect of LT in TR146 epithelial cell line, used to mimic oral mucosa,aimed at evaluating the expression of DEFB1, DEFB4, DEFB103 genes encoding for the human β-defensins 1, 2 and 3. In this cellular model LT decreased mRNA defensins' expression 30 minutes after irradiation but not 24 hours later, suggesting that LT is able to influence β-defensins production immediately after treatment, while its efficacy decreases over time...
February 21, 2018: Protein and Peptide Letters
Michael Gromiha, N R Siva Shanmugam, J Fermin Angelo Selvin, K Veluraja
Protein-carbohydrate interactions play vital roles in several biological processes in living organisms. The comparative analysis of binding site residues along with stabilizing residues in protein-carbohydrate complexes provides ample insights to understand the structure, function and recognition mechanism. In this work, we have identified 2.45% binding site residues in a non-redundant dataset of 1130 complexes using distance-based criteria and 7.07% stabilizing residues using the concepts of hydrophobicity, long-range interactions and conservation of residues...
February 21, 2018: Protein and Peptide Letters
Deepika Godugu, Karuna Rupula, Sashidhar Rao Beedu
Andrographolide, sourced from Andrographis paniculata, is an established therapeutic agent with variety of pharmacological properties in treatment of various diseases. The present study is designed to evaluate the interaction and binding affinity of andrographolide with HSA by docking and spectral studies. The docking study for screening the interaction of andrographolide with HSA protein was carried out using Auto Dock Vina software and the binding score of andrographolide was -8.7 kcal mol-1 and formed one hydrogen bond with Arg 218 residue of HSA in sub-domains IIA region...
February 11, 2018: Protein and Peptide Letters
Rajeev Yadav, Bhaswati Sengupta, Shyamashis Das, Pratik Sen
In the present work we have studied the interaction of two surfactants, an anionic (sodium dodecyl sulphate, SDS) and a cationic (cetyltrimethylammonium bromide, CTAB) with domain III of human serum albumin (HSA) using 8-anilino-1-naphthalene-sulphonate (ANS) as a fluorescent marker. The interaction of both the surfactants with HSA is found to be sequential in nature. The most important conclusion revealed from our study is that the nature of protein-surfactant interaction is not same throughout the entire protein...
February 11, 2018: Protein and Peptide Letters
Gopa Mahesh, Priyank Jaiswal, Sandip Dey, Jayati Sengupta, Sujoy Mukherjee
The serotonin 5HT2A G-protein coupled receptor (GPCR) is involved in a plethora of neuromodulatory functions and its dysregulation is linked to psychiatric disorders. However, there is insufficient, direct structural evidence of the structure, dynamics and the mechanism of function of this receptor. Cholesterol plays an important role in the function of many GPCRs and reduced cholesterol levels may be linked to serotonergic pathway disruptions. But cholesterol's effect on the structure, especially the oligomeric states of the 5HT2A receptor is not well understood...
February 6, 2018: Protein and Peptide Letters
Deniz Sahin, Sabriye Karadenizli, Murat Kasap, Berrin Oztas, Hale Maral Kir, Gurler Akpinar, Nurbay Ates
The role of intracellular proteins in the pathogenesis of absence epilepsy were mentioned. These proteins are thought to be related to energy generation, signal transduction, inflammation processes and membrane conductance. The investigation of protein profile of the genetically epileptic rat brains was the main subject of this study. For this, a 2D-gel electrophoresis based comparative proteome analysis was performed using thalamus tissue of genetic absence epileptic WAG/Rij and age matched Wistar rats. Regulated spots displaying differences in their abundance were identified using MALDI-TOF/TOF...
February 6, 2018: Protein and Peptide Letters
Marilia Cavalcanti Coriolano, Jessica de Santana Brito, Leydianne Leite de Siqueira Patriota, Ana Karine de Araújo Soares, Patricia Maria Guedes Paiva, Virginia Maria Barros de Lorena, Thiago Henrique Napoleao, Luana Barroso Coelho, Cristiane Moutinho Lagos de Melo
Moringa oleifera is used in traditional medicine as well as in food, cosmetic, and pharmaceutical industries. Water-soluble M. oleifera lectin (WSMoL) is an anionic protein isolated from the seeds. This work investigated the immunomodulatory effects of WSMoL on human PBMC. Measurement of cytokine and nitric oxide levels and immunophenotyping assays were investigated. PBMC were incubated with WSMoL (10 µg/mL) for 24 h, 48 h and 72 h, and was performed immunophenotyping of lymphocytes and monocytes. Culture supernatants were used to determined cytokine and nitric oxide levels...
January 30, 2018: Protein and Peptide Letters
Nurul Iman A Kameel, Adawiyah Suriza Shuib, Saad Tayyab
The stabilizing effect of different polyols (ethylene glycol, erythritol, xylitol and sorbitol) on the acid-denatured states of champedak galactose-binding (CGB) lectin at pH 2.5 (structured monomeric form) and pH 1.5 (acid-unfolded form) were studied using ANS fluorescence, Trp fluorescence and far-UV circular dichroism measurements. Presence of erythritol, xylitol and sorbitol in the incubation mixture was found to stabilize the CGB lectin at both pH 2.5 and pH 1.5, as evident from the burial of the hydrophobic clusters and decreased polarity around Trp residues...
January 30, 2018: Protein and Peptide Letters
Muhammad Rizwan Javed, Muhammad Hamid Rashid, Muhammad Riaz, Habibullah Nadeem, Muhammad Qasim, Nourin Ashiq
The extracellular β-glucosidases (BGL) from Aspergillus niger NIBGE (P) and its 2-Deoxy-D-glucose resistant mutant derivative NIBGE-06 (M-6), produced under submerged growth conditions were purified to homogeneity level by ammonium sulfate precipitation and FPLC system. The BGLs from both strains were dimeric in nature, with subunit and native molecular masses of 130 kDa and 252 kDa, respectively. The comparative analysis of nucleotides of bgl genes revealed 8 point mutations within the bgl gene of mutant strain as compared to parental strain...
January 30, 2018: Protein and Peptide Letters
Shabih Fatma, Amir Hameed, Muhammad Noman, Temoor Ahmed, Imran Sohail, Muhammad Shahid, Mohsin Tariq, Romana Tabassum
Uprising energy crisis is predicted to be critically challenging in the foreseeable future where existing fossil fuel reservoirs are fast depleting and their extensive usage is posing serious health-related concerns to biological life on earth. To cover up these exhaustible energy resources, extensive efforts are going on to discover new frontiers in generating alternative energy sources on sustainable, renewable, and most importantly environment-friendly basis. In this context, biofuels (biomasses derived fuels) are seemed to be a promising solution considering the economical and applicable aspects...
January 22, 2018: Protein and Peptide Letters
Jianren Xu, Xinqing Zhao, Chenguang Liu, Fengwu Bai
Saccharomyces cerevisiae cannot efficiently consume xylose and other pentose sugars derived from hemicellulose hydrolysis. Although engineering the yeast strains with xylose metabolic pathway has been intensively studied, challenges are still ahead for developing robust strains for bioethanol production from lignocellulose. In this study, the role in xylose utilization of the mutated MIG1 for glucose repression in S. cerevisiae was studied. No difference was observed for S288c growing on Petri dishes supplemented with xylose when it was engineered with the overexpression or deletion of its native MIG1, but the growth was enhanced when overexpressing the MIG1 mutant from the self-flocculating yeast SPSC01...
January 22, 2018: Protein and Peptide Letters
Sana Malik, Ifrah Afzal, Muhammad Aamer Mehmood, Sawsan Abdulaziz Rahimuddin, Munazza Gull, Nazia Nahid
Increasing global demands for drop-in biofuels along with raising environmental concerns have led us to develop sustainable, renewable and cost-effective liquid fuels for transport and industrial sector. Production of a fuel that is economical and compatible with existing infrastructure is crucial. While the lower titer and difficult recovery of the advanced biofuels are the key challenges in this context. By employing metabolic engineering techniques and synthetic biology tools, scientists have developed a variety of potential microbial strains with improved yield of biofuels...
January 22, 2018: Protein and Peptide Letters
Muhammad Sarwar Khan, Ghulam Mustafa, Faiz Ahmad Joyia
Enzymes are one of the most important entities for life. A number of enzymes are isolated from organisms of diverse origin, characterized and over-expressed in planta including, other expression systems. Genes are expressed and enzymes accumulate to high levels with a bona fide structure in plants. Further, plants provide inexpensive production platforms for pharmaceuticals and nutraceuticals given that engineering of plant genomes provides long-term storage without the requirements of cold chain logistics...
January 22, 2018: Protein and Peptide Letters
Wei Zhang, Jigang Yu, Xuecheng Zhang, Hui Peng, Xinxin Li, Jiahai Zhang, Hongbin Sun, Xiaoming Tu
AmyP is an α-amylase which shows preferential degradation to soluble starch. In this substrate preference its starch binding domain (SBD), which was recently assigned to a new carbohydrate binding module (CBM) family 69, plays an important role. In the present study, the SBD of AmyP (AmyP-SBD) was recombinantly expressed, purified, and structurally characterized. Using circular dichroism (CD), intrinsic fluorescence, and nuclear magnetic resonance (NMR) spectroscopy, the structures of AmyP-SBD in the absence and presence of substrate analogue β-cyclodextrin were measured...
January 22, 2018: Protein and Peptide Letters
Muhammad Omar Khan, Muhammad Aamer Mehmood, Zahid Mukhtar, Niaz Ahmad
Chloroplasts are vital photosynthetic organelles in plant cells that carry out several important cellular functions including synthesis of amino acids, fatty acids, and lipids and metabolism of nitrogen, starch, and Sulphur to sustain the homeostasis in plants. These organelles have got their own genome, and related genetic machinery to synthesize required proteins for various plant functions. Genetic manipulations of the chloroplast genome for different biotech applications has been of great interest due to desired features including the availability of operonal mode of gene expression, high copy number, and maternal mode of inheritance (in the most field crops)...
January 21, 2018: Protein and Peptide Letters
Hamid Mukhtar, Syed M Suliman, Aroosh Shabbir, Muhammad W Mumtaz, Umer Rashid, Sawsan A Rahimuddin
Fifty yeast strains were isolated from soil and eleven of them were selected for oil production. Five yeast strains were designated as oleaginous because they produced more than 16% of oil based on their biomass. The isolate IIB-10 identified as Yarrowia lipolytica produced maximum amount of lipids i.e. 22.8%. More amount of biomass was obtained when cane molasses was utilized as carbon source where it produced 29.4 g/L of biomass while sucrose and lactose were not utilized by IIB-10 and no biomass was obtained...
January 21, 2018: Protein and Peptide Letters
Kai Li, Jia-Wei Zhang, Chen-Guang Liu, Feng-Wu Bai
The present study investigated the effects of formic acid, acetic acid, furfural, 5-HMF, and ethanol on activity of two commercial cellulases from Novozyme and Youtell. The carboxylic acid (formic acid and acetic acid) showed little impact on cellulose hydrolysis, but furan derivate (furfural, 5-HMF) performed higher inhibitory effects. The significant decrease of enzyme activity (Novozyme 84%, Youtell 75.8%) happened as addition of 6 g/L furfural. The synthetic solution containing four inhibitors with similar concentration as the acid-pretreated corn stover hydrolysate decreased enzyme activity by ~10%...
January 21, 2018: Protein and Peptide Letters
Muhammad Bilal, Muhammad Zohaib Nawaz, Hafiz M N Iqbal, Jialin Hou, Shahid Mahboob, Khalid A Al-Ghanim, Cheng Hairong
Raising environmental concerns and recent global scenario of cleaner production and consumption are leading the world to design green industrial processes to produce alternative fuels and chemicals. Lignocellulosic biomass is enormous and renewable biological resource to produce ethanol and chemicals on sustainable and environmentally basis. Although, bioethanol is one of the most promising and eco-friendly alternatives to fossil fuels yet its production from food and feed has received much negative criticism...
January 21, 2018: Protein and Peptide Letters
Ifrah Afzal, Muhammad Sajjad Ahmad, Sana Malik, Muhammad Ibrahim, Omar Salim Al-Ayed, Ghulam Qadir, Huda Al Doghaither, Munazza Gull
Eichhornia crassipes is an aquatic plant well known for its role in soil reclamation due to the containment of valuable nutrients. Moreover, its biomass is an abundant and low-cost biological resource. While pyrolysis of a biomass offers one of the cleanest methods to harness the bioenergy stored in the biomass. The present study was focused on evaluating the bioenergy potential of Eichhornia crassipes via pyrolysis. Biomass of E. crassipes was collected from a municipal wastewater pond. Oven dried powdered biomass of E...
January 21, 2018: Protein and Peptide Letters
Aqib Zafar Khan, Ayesha Shahid, Hairong Cheng, Shahid Mahboob, Khalid A Al-Ghanim, Muhammad Bilal, Muhammad Zohaib Nawaz
Microalgae have been appeared as promising feedstocks of significant biotechnological interest due to their enormous potential for the sustainable production of industrially valuable compounds such as lipids/fatty acids, proteins, metabolites, pigments, and biofuels. However, exploitation of algal biomass for commercial purposes is still in infancy due to the dearth of the knowledge regarding state-of-the-art sophisticated technologies. Inarguably, over the past several years, biological sciences have witnessed an explosion of innovative strategies enabling scientific community and research-based organizations to scrutinize entire classes of biomolecules from a cell type or whole organism, collectively titled as 'Omics, including genomics, transcriptomics, proteomics and lipidomics...
January 21, 2018: Protein and Peptide Letters
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"