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Protein and Peptide Letters

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https://www.readbyqxmd.com/read/28641564/investigation-on-the-interaction-of-pseudomonas-syringae-effector-avrpto-with-atrabe1d-gtpase
#1
Xiaomin Hou, Yi Gao
The tomato bacterial speck is a worldwide disease. It is caused by the infection of pathogenic pseudomonas syringae pv. tomato which delivers the effector AvrPto into the host cells via the type III secretion system. AvrPto interacts Rab8 subfamily protein in the GTP-bound form and participates in the response to pathogen infection, but the pathogenic mechanism involved by Rab8 subfamily remains elusive. Here, we investigated on the interrelationship of AvrPto8-159 with Arabidopsis thaliana GTP-restricted Rab8 subfamily protein AtRabE1d13-185Q74L through some biophysical and biochemical methods...
June 21, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28641563/pentapeptide-prosequence-enhances-expression-and-structure-folding-of-recombinant-thermomyces-lanuginosus-lipase-in-pichia-pastoris
#2
Haiying Cai, Minjie Zhao, Yang Li, Jianwei Mao, Chenggang Cai, Fengqin Feng
Propeptides have been demonstrated to influence many properties of their mature proteins as intramolecular chaperones. In our study, the role of pentapeptide prosequence of Thermomyces lanuginosus lipase (TLL) was determined through its effect on the recombinant expression of TLL in P. pastoris. The result showed that the average lipase activity of recombinant strains GS-pTL reached 434.32 U/mL, higher than that of GS-TL 377.71 U/mL, indicating that the propeptide "SPIRR-" sequence is beneficial for enhancing TLL expression...
June 21, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28641565/d-amino-acid-analogues-of-the-antimicrobial-peptide-cdt-exhibit-anti-cancer-properties-in-a549-a-human-lung-adenocarcinoma-cell-line
#3
Deborah L Heyl, Hedeel Guy Evans, Jeff Guthrie, Murali Jujjavarapu, Nathan Hendrickson, Anna Eitel, Yeji Park, Jennifer Garvey, Rebecca Newman, Daniel Esckilsen
The importance of the antitumor activity of some antimicrobial peptides is being increasingly recognized. The antimicrobial peptide, tachyplesin, has been shown to exhibit anticancer properties and a linear, cysteine deleted analogue (CDT), was found to retain its antibacterial function. We used sequence reversal and D-amino acids to synthesize four CDT analogues. We found that D-CDT exhibited the best bactericidal properties with the highest selectivity index. While the MIC was found to improve only modestly against S...
June 20, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28641562/structural-characterization-of-amyloid-%C3%AE-17-42-dimer-by-potential-of-mean-force-analysis-insights-from-molecular-dynamics-simulations
#4
Mary Dutta, Rajkalyan Chutia, Venkata Satish Kumar Mattaparthi
Recent experiments with Amyloid β1-42 peptide have indicated that the initial dimerization of Aβ1-42 monomers to form amyloid dimers stand out as a key event in the generation of toxic oligomers. However, the structural characterization of Aβ1-42 dimer at the atomistic level and the dimerization mechanism by which Aβ1-42 peptides co-aggregate still remains not clear. In the present study, the process of Aβ17-42 peptide dimerization which is known to play an important role in the plaque formation was evaluated in terms of potential of mean force, which provided free energies along the reaction coordinates...
June 20, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28641561/induction-of-humoral-and-cellular-immune-responses-in-mice-by-multiepitope-vaccines-composing-of-both-t-and-b-lymphocyte-epitopes-of-mage-a3which-are-recombined-into-hbcag
#5
Qingxin Chen, Wenshu Li, Pengfei Wang, Huanyi Shao, Yujie Ding, Wenhuan Wang, Danwei Cen, Yiqi Cai, Xiangyang Xue, Lifang Zhang, Guanbao Zhu
MAGE-A3 is highly expressed in many kinds of malignant diseases, and considered to be an ideal candidate for anti-tumor vaccine. To improve the immunogenicity of epitopes of MAGE-A3, hepatitis B virus core antigen (HBcAg) was used as an immune-carrier by insertion epitopes at HBcAg major immunodominant region (HBcAg(MIR)). In present study, we designed three kinds of MAGE-A3 multiepitopes containing T and B-cell dominant epitope-rich peptides, and constructed three recombinant chimeras of HBcAg(MIR)/MAGE-A3(EPI-1), HBcAg(MIR)/MAGE-A3(EPI-2), and HBcAg(MIR)/MAGE-A3(EPI-3) with prokaryotic codon optimization...
June 20, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28641560/functional-and-catalytic-characterization-of-the-detoxifying-enzyme-haloalkane-dehalogenase-from-rhizobium-leguminosarum
#6
Nikolaos Georgakis, Evangelia Chronopoulou, Maria Anna Gad, Dimitrios Skliros, Rodica Efrose, Emmanouil Flemetakis, Nikolaos E Labrou
Haloalkanes are acutely toxic to animals and humans and their toxic effects are mainly observed in the liver, kidneys and central nervous system. In the present work, the haloalkane dehalogenase from Rhizobium leguminosarum bv. trifolii (DrlA) was characterized. Reverse transcription polymerase chain reaction analysis and enzyme activity assays revealed that the DrlA gene expression in R. leguminosarum bv. trifolii is induced by 1,2-dibromoethane (1,2-DBE) during the early exponential phase. The gene of the enzyme was isolated, cloned and expressed in E...
June 20, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28641559/a-stepwise-approach-for-the-isolation-and-the-identification-of-chemically-reactive-exofacial-protein-thiols
#7
Gabriele Candiani, Elisa Fasoli
The plasma membrane controls the selective internalization of (macro)molecules through different mechanisms, often relaying on specialized outward-facing carriers such as exofacial proteins thiols (EPTs). Although the interchange of critical thiols and disulphides between EPTs and exogenous cargoes is the first critical event, the identification of specific cell interactors remains to be thoroughly explored. We herein devise an enabling methodology for the isolation and identification of a subset of EPTs, named chemically reactive EPS, which are potential theranostic targets...
June 20, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28464764/the-relation-between-lipase-thermostability-and-dynamics-of-hydrogen-bond-and-hydrogen-bond-network-based-on-long-time-molecular-dynamics-simulation
#8
Leiyu Zhang, Yanrui Ding
Hydrogen Bonds (HBs) play an important role in stabilizing the protein. Long time molecular dynamics simulation was performed at different temperatures to study the dynamics of HBs and HB networks of Wild Type Lipase (WTL) (Tm=56℃) and mutant 6B (Tm = 78.2℃). The persistence values of HBs formed by mutations A15S, A20E, G111D, M137P and N166Y are different from those of WTL, especially for mutation N166Y. The higher HB persistence value generally means that the HB is more stable. These mutations directly improve the stability of these HBs referring to their persistence values, which show that mutations strengthen the ability of HBs to withstand high temperature and then stabilize the secondary structure...
May 2, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28462713/antimicrobial-and-immunomodulatory-activity-of-pmap-23-derived-peptides
#9
Edwin J A Veldhuizen, Maaike R Scheenstra, Johanna L M Tjeerdsma-van Bokhoven, Maarten Coorens, Viktoria A F Schneider, Floris J Bikker, Albert van Dijk, Henk P Haagsman
The Porcine Myeloid Antibacterial Peptide (PMAP)-23 is a porcine host defence peptide with described strong antibacterial activity against Gram-positive and Gram-negative bacteria, and fungi. In this study, PMAP-23 and short and/or mutated derivatives were tested for antibacterial and immunomodulatory activities to determine core elements of the peptide required for functionality. Shortening of the peptide on either N- or C-terminus did not lead to a strong reduction in antibacterial activity, but interestingly, all C-terminal truncated forms were strongly inhibited by salt addition...
April 28, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28462712/improved-hydrolysis-of-organophosphorus-compounds-by-engineered-human-prolidases
#10
Hyeongseok Yun, Sungrae Lee, Sumi Kim, Jiyeon Yu, Nari Lee, Jinhee Lee, Nam Doo Kim, Chiho Yu, Jaerang Rho
Human prolidase is a proline-specific metallopeptidase that catalyzes the hydrolysis of dipeptides containing a proline residue at the C-terminus. Human prolidase also has weak hydrolytic activity for toxic organophosphorus compounds, including diisopropyl fluorophosphate (DFP), sarin and soman. In this study, we performed in silico analysis to improve the catalytic activity of human prolidase towards DFP hydrolysis. We predicted that single amino acid substitution mutations of either A252R or P365R could form a salt bridge between the A252R mutant residue and the E249 residue or the P365R mutant residue and the E424 residue...
April 28, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28462722/the-regulatory-functions-of-a-new-tetrapeptide-from-the-bursa-of-fabricius-on-aiv-vaccine-immunization-and-antibody-production
#11
Man Man Zong, Chuan Jie Zhou, Yuan Nan Yu, Yang Zheng, Xiu Li Feng, Pu Yan Chen, Mei Yang
The bursa of Fabricius (BF) is the acknowledged humoral central immune organ and plays vital role on B lymphocyte development. In the study, a new tetrapeptide of GERA isolated from BF was named bursal peptide tetrapeptide (BP4). To investigate the immunomodulatory function of BP4 on antigen-specific immune responses, BALB/c mice were immunized with inactivated influence virus (AIV, H9N2 subtype) vaccine and BP4 at three experimental dosages. The results showed that BP4 enhanced the increased AIV-specific antibody and cytokine production, and modified T cell subtype population...
April 25, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28462721/the-expression-and-purification-of-the-delta-sleep-inducing-peptide-fused-with-protein-transduction-domain-and-human-serum-albumin-in-pichia-pastoris
#12
Xin-Guo Zhang, Wen-Na Wang, Chun-Sheng Zhang, Kun Li, Guo-Di Ma, Jian-Yong Li
Delta sleep-inducing peptide (DSIP) is a nonapeptide that could promote sleep through the induction of slow wave sleep. To further study the pharmacological effect of DSIP on insomnia, we designed a fusion protein containing N-terminal TAT-based transduction domain followed by human serum albumin and DSIP and designated this protein as PHD fusion protein. We expressed PHD fusion protein in Pichia pastoris, purified it to near homogeneity by three-step chromatography and performed bioactivity assay. The pharmacological activity of PHD fusion protein was studied using classic pentobarbital-induced sleep test in healthy mice...
April 25, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28425860/a-novel-recombinant-multi-epitope-vaccine-could-induce-specific-cytotoxic-t-lymphocyte-response-in-vitro-and-in-vivo
#13
Yahong Wu, Wenjie Zhai, Meng Sun, Zhe Zou, Xiuman Zhou, Guodong Li, Zhongyi Yan, Yuanming Qi, Yanfeng Gao
Cytotoxic CD8+ T lymphocytes (CTLs) play an important role in killing tumor cells. To develop therapeutic vaccines that can induce specific immune response, we designed a novel recombinant multiepitope vaccine YL66 which consists of HLA-A2-restricted CTL epitopes from two widely expressed tumor antigen cyclooxygenase-2 and MAGE-4, linked with membrane permeable Tat-PTD and the universal T helper epitope. The DNA fragment of YL66 was cloned into pGEX-4T-2-YL66 and pcDNA3.1(+)-YL66, respectively. Then, we tested whether the expressed protein GST-YL66 and DNA vaccine pcDNA3...
April 19, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28425861/role-of-cysteine-residues-in-regulation-of-peptidyl-prolyl-cis-trans-isomerase-activity-of-wheat-cyclophilin-tacypa-1
#14
Gundeep Kaur, Harpreet Singh, Kirandeep Kaur, Suchismita Roy, Ashwani Pareek, Prabhjeet Singh
Oxidative conditions result in inhibition of peptidyl-prolyl cis-trans isomerase (PPIase) activity of several cyclophilins. Thiol groups have been implicated in redox regulation of these proteins. In our previous study, we proposed that activity of wheat cyclophilin, TaCYPA-1, may be modulated through a novel dual mechanism of redox regulation. To further understand the regulation of PPIase activity of TaCYPA-1, we generated mutants of TaCYPA-1 by substituting cysteine residues at positions -40 and -122 with serine, and at -126 with proline...
April 17, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28413971/structure-of-c-terminal-domain-of-peptidyl-prolyl-cis-trans-isomerase-from-pseudomonas-syringae-pv-tomato-str-dc3000-at-1-6%C3%A3-resolution
#15
Yu Gao, Hong-Mei Zhang, Piwu Wang, Mei Li
Peptidyl-prolyl cis-trans isomerase (PPIase) catalyzes the conversion between the cis and trans configurations of proline which switches the backbone conformation of peptides. We have determined the crystal structure of the C-terminal domain of a PPIase from Pseudomonas syringae pv. tomato DC3000 (PSPTO DC3000) at 1.6 Å resolution. Structural comparison of PSPTO-PPIase with its homologues suggests that the successive arrangement of residues with short side chains in loop 4 contributes to its substrate specificity...
April 13, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28412901/an-analysis-of-central-residues-between-ligand-bound-and-ligand-free-protein-structures-based-on-network-approach
#16
Amala Arumugam, Arnold Emerson Isacc
Depiction of protein structures as networks of interacting residues has enabled us to understand the structure and function of the protein. Previous investigations on closeness centrality have identified protein functional sites from three- dimensional structures. It is well recognized that ligand binding to a receptor protein induces a wide range of structural changes. An interesting question is, how central residues function during conformational changes triggered during ligand binding? The aim of this study is to comprehend at what extent central residues change during ligand binding to receptor proteins...
April 13, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28393686/impact-of-salt-concentration-and-ph-on-surface-charged-residues-controlling-protein-association-pathways-in-carboxylesterase-estgta2
#17
Jessica K Moisan, Fatma Meddeb-Mouelhi, David M Charbonneau, Marc Beauregard
Understanding the relationship between enzymatic stability and the amino acid sequence encoding such enzymes is of utmost importance. Here we thoroughly characterized the behavior of the carboxylesterase EstGtA2 from Geobacillus thermodenitrificans during thermal denaturation at different pH with various salt concentrations. EstGtA2 was characterized by circular dichroism regarding conformation and thermal stability, by dynamic light scattering for detection of association/aggregation, by enzymatic assays for activity, and by monitoring the impact of heat treatments on activity...
April 4, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28393685/immunoproteomics-approach-for-screening-vaccine-candidates-against-the-intestinal-botulism
#18
Arti Sharma, Sarkaraisamy Ponmariappam, Sarita Rani
Intestinal botulism is an infectious form of botulism in which disease results from ingesting spores, which is followed by spore germination and intraluminal production of botulinum neurotoxins over an extended period. Botulinum neurotoxin is produced by endospore forming bacteria called C. botulinum. The immunoproteomic is used to screen the cross reactive immunogenic proteins of C. botulinum type B using C. botulinum type B live spore antiserum. The whole cell proteins are separated by two dimensional gel electrophoresis and transferred to polyvinylidene difluoride membranes...
April 4, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28393684/generation-of-the-fluorescent-hmgb1-gfp-fusion-protein-in-insect-cells-and-evaluation-of-its-immunogenicity-in-two-mice-models
#19
Ali Anvar, Rouhollah Vahabpour, Iman Salahshourifar, Azam Bolhassani
High mobility group box 1 (HMGB1) is a highly conserved protein present in the nuclei and cytoplasm of cells which has an important role as a mediator of inflammation in the extracellular environment. HMGB1 was identified as an innate adjuvant that induces immune responses against soluble antigens in vivo. In the current study, we used a baculovirus expression system for insect cells that was based on expression of HMGB1 with target gene (GFP), and purified the recombinant HMGB1-GFP fusion protein. We then demonstrated whether immunogenicity of GFP changes in the presence or absence of recombinant HMGB1 acting as an adjuvant in C57BL/6 and BALB/c mice...
April 4, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28393683/identification-and-characterization-of-strychnine-binding-peptide-using-phage-display-screening
#20
Fang Zhang, Min Wang, Zheng Qiu, Xiao-Meng Wang, Chun-Lei Xu, Xia Zhang
Phage display has been proved in drug development as a high-throughput method for identifying specific cellular target of different drugs. However, phage display remains an intricate process for insoluble small chemicals because of the difficulty of presenting the molecules to the phages without occupying or destroying the very limited functional groups. In the present study, with Strychnine (Stry) selected as a model compound, we have developed an alternative in vitro bio-panning method against insoluble suspension...
April 4, 2017: Protein and Peptide Letters
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