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Protein and Peptide Letters

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https://www.readbyqxmd.com/read/28799506/antibody-epitope-analysis-to-investigate-folded-structure-allosteric-conformation-and-evolutionary-lineage-of-proteins
#1
Sienna Wong, J-P Jin
Study of folded structure of proteins provides insights into their biological functions, conformational dynamics and molecular evolution. Current methods of elucidating folded structure of proteins are laborious, low-throughput, and constrained by various limitations. Arising from these methods is the need for a sensitive, quantitative, rapid and high-throughput method not only analyzing the folded structure of proteins, but also to monitor dynamic changes under physiological or experimental conditions. In this focused review, we outline the foundation and limitations of current protein structure-determination methods prior to discussing the advantages of an emerging antibody epitope analysis for applications in structural, conformational and evolutionary studies of proteins...
August 11, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28799505/single-molecular-level-probing-of-structure-and-dynamics-of-papain-under-denaturation
#2
Bhaswati Sengupta, Apala Chaudhury, Nilimesh Das, Pratik Sen
INTRODUCTION: Papain is a cysteine protease enzyme present in papaya and known to help in digesting peptide. Thus the structure and function of the active site of papain is of interest. OBJECTIVE: The objective of present study is to unveil the overall structural transformation and the local structural change around the active site of papain as a function of chemical denaturant. METHODS: Papain has been tagged at Cys-25 with a thiol specific fluorescence probe N-(7-dimethylamino-4-methylcoumarin-3-yl) iodoacetamide (DACIA)...
August 11, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28799504/glycan-phosphorylases-in-multi-enzyme-synthetic-processes
#3
Robert Field, Giulia Pergolizzi, Sakonwan Kuhaudomlarp, Eeshan Kalita
Glycoside phosphorylases catalyse the reversible synthesis of glycosidic bonds by glycosylation with concomitant release of inorganic phosphate. The equilibrium position of such reactions can render them of limited synthetic utility, unless coupled with a secondary enzymatic step where the reaction lies heavily in favour of product. This article surveys recent works on the combined use of glycan phosphorylases with other enzymes to achieve synthetically useful processes.
August 11, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28782479/candida-infections-and-human-defensins
#4
Vania Polesello, Ludovica Segat, Sergio Crovella, Luisa Zupin
Candida species infections are an important worldwide health issue since they do not only affect immunocompromised patients but also healthy individuals. Indeed Candida spp. are present as commensal flora in the host human body but they can switch into a pathological form inducing cellular damage and disease. Among Candida strains, C. albicans is the most prevalent in both mucosal and systemic infections. The host developed different mechanisms of protection against Candida infections; specifically the immune system and the innate immune response are the first line of defence...
August 7, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28782478/a-comparative-study-of-the-impact-of-calcium-ion-on-structure-aggregation-and-chaperone-function-of-human-%C3%AE-a-crystallin-and-its-cataract-causing-r12c-mutant
#5
Reza Yousefi, Sadaf Saba, Maryam Ghahramani
Several mutations in the genes encoding α-crystallin (α-Cry) subunits have been indicated to cause cataract diseases in human. Also, elevation in calcium level has been shown to induce aggregation of α-Cry which is accompanied with a significant reduction in its chaperone function. In some pathological conditions, the simultaneous incidence of α-Cry mutation and lens electrolyte imbalance might exacerbate the cataract phenotype. Therefore, in this study, the impact of calcium ion was investigated on structure, chaperone activity and amyloidogenic properties of the recombinant wild-type and mutant R12C αA-Cry, in a comparative study...
August 7, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28758598/synthetic-peptides-derived-from-ribosomal-proteins-of-leishmania-spp-in-mucocutaneous-leishmaniasis-diagnostic-usefulness
#6
Magda Melissa Florez, Camila Indiani de Oliveira, Concepción Puerta, Fanny Guzmán, Martha Ayala, Gladis Montoya, Gabriela Delgado
The serological diagnostic methods currently available for mucocutaneous leishmaniasis (MCL) lack specificity when complete parasites are used; however, such specificity increases when protein fractions are used. Ribosomal proteins have been reported to induce antibodies in animal and humans infected with the parasite, making them a worth candidate to assess its diagnosis potential. This study was thus aimed at evaluating synthetic peptides derived from Leishmania braziliensis ribosomal proteins S25 and S5 as antigen candidates for diagnosing MCL by ELISA; 4 of these 21 peptides (P4, P6, P19 and P21) had the greatest sensitivity (21...
July 28, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28758597/preterm-birth-and-renin-angiotensin-aldosterone-system-evidences-of-activation-and-impact-on-developmental-cardiovascular-disease-risks
#7
Mariane Bertagnolli
Preterm birth (< 37 gestational weeks) reaches 10% of total births worldwide. Early exposure to an ex utero environment can alter organogenesis and maturation in the newborn. This early onset of events can further promote long-term developmental alterations and cardiovascular disease risks. Mechanisms activated during preterm birth and promoting such cardiovascular alterations have just recently been investigated. As a major candidate, the renin angiotensin aldosterone system (RAAS) can be acutely altered during preterm birth and persistently activated in later life...
July 28, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28758596/effects-of-sleeve-gastrectomy-on-the-metabolic-profile-and-on-the-expression-of-renin-angiotensin-system-in-adipose-tissue-of-obese-rats
#8
Thaísa Soares Crespo, João Marcus Oliveira Andrade, Alanna Fernandes Paraíso, Deborah de Farias Lelis, Pablo Vinicyus Ferreira Chagas, Antônio Sérgio Barcala Jorge, Wagner Leite Ferreira, Alfredo Maurício Batista de Paula, André Luiz Sena Guimarães, Sérgio Henrique Sousa Santos
Sleeve gastrectomy (SG) has been used as a multipurpose surgical procedure for the treatment of obesity. The present study assessed the effects of SG on the metabolic and inflammatory profile and renin-angiotensin system (RAS) expression in the white adipose tissue of male rats with obesity induced by a high-fat diet. Male Wistar rats were treated with a standard diet or high-fat diet and submitted to SG or sham surgery. The glycemic and lipid profiles and gene expression of inflammatory markers and RAS components in adipose tissue were evaluated...
July 28, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28758595/vasodilator-effect-of-angiotensin-1-7-on-vascular-coronary-bed-of-rats-role-of-mas-ace-and-ace2
#9
Patrícia Lanza Moraes, Lucas Miranda Kangussu, Carlos Henrique Castro, Robson As Santos, Anderson José Ferreira, Alvair P Almeida
Angiotensin(Ang)-(1-7) is a biologically active member of the renin-angiotensin system that participates of the regulation of blood pressure. Although Ang-(1-7) is able to potentiate the vasodilator effect of bradykinin in coronary bed of rats, a direct vasodilator effect of Ang-(1-7) in this vascular bed has not been characterized. Thus, the aim of this study was to evaluate the mechanisms involved in the vasodilator effect of Ang-(1-7) in the vasculature of isolated rat hearts perfused according to the Langendorff technique at constant flow...
July 28, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28758594/effects-of-resveratrol-and-ace-inhibitor-enalapril-on-glucose-and-lipid-profiles-in-mice
#10
Thaisa de Almeida Pinheiro, Thales de Almeida Pinheiro, Jonh David Feltenberger, João Marcus Oliveira Andrade, Emillio César Ferreira, Deborah De Farias Lelis, Andre Luiz Sena Guimarães, Alfredo Maurício Batista de Paula, Antônio Prates Caldeira, Sergio Sousa Santos
Lipogenesis is a process that involves the synthesis of fatty acids. Resveratrol and enalapril are being studied for their beneficial physiological properties on the glucose and lipid metabolism. The aim of the present study was to evaluate the oral administration of resveratrol and enalapril on the glucose and lipid metabolism, evaluating the white pad lipogenesis genes expression in mice. Swiss male mice were divided into four groups and treated for eight weeks as follows: Standard diet ad libitum (G1); Standard diet + Resveratrol (G2); Standard diet + Enalapril (G3); Standard diet + Resveratrol + Enalapril (G4), where resveratrol was administered with the food and enalapril with the water...
July 28, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28758593/physical-exercise-and-ace2-angiotensin-1-7-mas-receptor-axis-of-the-renin-angiotensin-system
#11
Albená Nunes-Silva, Guilherme Carvalho Rocha, Daniel Massote Magalhaes, Lucas Neves Vaz, Marcelo Henrique Salviano Faria, Ana Cristina Simões E Silva
Many physiological responses of the Renin Angiotensin System (RAS) are associated with two opposite pathways: (1) a classical one formed by angiotensin-converting enzyme (ACE), Angiotensin II (Ang II) and Angiotensin type 1 (AT1) receptor, which is associated to vasoconstriction, cell proliferation, organ hypertrophy, sodium retention and aldosterone release and (2) a counter-regulatory or vasodilator pathway comprising angiotensin-converting enzyme 2 (ACE2), Angiotensin-(1-7) [Ang-(1-7)] and Mas receptor, which is involved in vasodilation, anti-proliferation, anti-hypertrophy, cardioprotective and renoprotective actions...
July 28, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28758592/the-angiotensin-converting-enzyme-2-ace2-gut-microbiota-and-cardiovascular-health
#12
João Marcus Oliveira Andrade, Deborah de Farias Lelis, Valeria Mafra, Junio Cota
The renin-angiotensin system (RAS) is an important enzymatic system responsible for the regulation of biological functions, such as the arterial pressure, hydroelectrolytic control and vascular vasodilatation/vasoconstriction. The RAS component ACE2 acts as a mediator in vasoprotection, and it is highly expressed in the kidney, heart, and testis. Recently, the association between the ACE2 and gut microbiota has been discussed. It is shown that the ACE2/Ang-(1-7) axis modulates the immune response, influencing the microbiota composition, and thus being one of the causes for some diseases physiophatologies, such as diarrhea and intestinal inflammatory disease...
July 28, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28758591/brain-angiotensin-ii-involvement-in-chronic-mental-disorders
#13
Osvaldo Martin Basmadjian, Victoria Belen Occhieppo, Natalia Andrea Marchese, Gustavo Baiardi, Claudia Bregonzio
The functioning of the central nervous system is complex and it implies tight and coordinated interactions among multiple components. Neurotransmitters systems imbalance is a hallmark in central nervous system (CNS) disorders. These pathologies profoundly impact on social, cultural, and economic perspective worldwide. The etiopathology of CNS illnesses is still poorly understood making their treatment difficult. Brain angiotensin II (Ang II), through its AT1 receptors, modulates dopaminergic, glutamatergic and GABAergic neurotransmission, which are responsible for movement control, cognition, emotions and stress responses...
July 28, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28758590/renin-angiotensin-system-in-diabetes
#14
Michael Bader, Johannes Rein
The renin-angiotensin system (RAS) has two different axes, the classical one with the effector peptide angiotensin II and the new one with the effector peptide angiotensin (1-7). Both peptides have been shown to be involved in the pathogenesis of diabetes mellitus and its consequences, nephropathy, retinopathy and cardiomyopathy in animal models and patients. In diabetes, angiotensin II acts mostly deleterious and angiotensin (1-7) protective. In this review we summarize the knowledge about the role of the different RAS axes in diabetes mellitus and the use of drugs interfering with the RAS in the therapy of the disease...
July 28, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28741467/status-quo-in-antibody-drug-conjugates-can-glyco-enzymes-solve-the-current-challenges
#15
Florentina Kubizek, Britta Eggenreich, Oliver Spadiut
Over the last years, a novel class of anti-cancer drugs named antibody-drug conjugates (ADCs) has been developed. Due to their limited off-target toxicity but highly potent cytotoxicity at tumor sites, ADCs have proven to be a good alternative to ordinary cancer treatment, such as chemotherapy or combination therapy. Numerous enhancements in antibody-drug engineering led to highly potent tumor targeting drugs with a wide therapeutic window. Two ADCs (Brentuximab vedotin and Trastuzumab emtansine) are already on the market and many others in clinical trials...
July 24, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28741466/binding-of-cxcr4-transmembrane-peptides-to-the-bacterial-chaperonin-groel
#16
Xiaoqiang Wang, Haixia Chi, Shixin Li, Yan Xu
: Newly translated membrane protein CXCR4 was observed to gain significant folding enhancement upon interacting with the bacterial chaperonin GroEL-GroES. The main fold of CXCR4 consists of 7 transmembrane α-helices distinct from soluble proteins that have dominated research on the chaperonin-assisted folding. This work extends our previous observation to the binding of GroEL with CXCR4 transmembrane peptides, which are mainly composed of hydrophobic residues characteristic of the substrates recognized by the chaperonin...
July 24, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28741465/engineering-of-chimeric-protein-based-on-e-protein-domain-iii-of-tick-borne-encephalitis-virus-and-ompf-porin-of-yersinia-pseudotuberculosis
#17
Anna M Stenkova, Natalia S Chopenko, Ludmila A Davydova, Andrey N Mazeika, Evgeniya P Bystritskaya, Olga Y Portnyagina, Stanislav D Anastyuk, Dmitrii S Kulbatskii, Ekaterina N Lyukmanova, Dmitriy A Dolgikh, Eduard Y Kostetsky, Nina M Sanina
Tick-borne encephalitis poses a serious public health threat in the endemic regions. The disease treatment is restricted to symptomatic therapy, so great expectations are in the development of the prophylactic and therapeutic vaccines. The domain III of E protein of the tick-borne encephalitis virus is the main antigenic domain which includes virus-specific epitopes recognized by neutralizing antibodies. We have expressed, isolated and characterized the chimeric protein based on the fusion of domain III of E protein of the tick-borne encephalitis virus and bacterial porin OmpF from Yersinia pseudotuberculosis...
July 24, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28741464/expression-of-mastoparan-b-a-venom-peptide-via-escherichia-coli-c43-de3-coupled-with-an-artificial-oil-body-cyanogen-bromide-technology-platform
#18
Tzyy-Rong Jinn, Sheng-Kuo Hsieh, Yu-Jen Yu, Nou-Ying Tang, Jhao-Ren Lin
Here, we successfully use the oleosin-based fusion expression strategy coupled with the artificial oil body (AOB)-cyanogen bromide (CNBr) platform to produce bioactive MPB peptide which, in a manner identical to that of its native counterpart. In this study, the MPB peptide was interlinked with recombinant oleosin (rOle(127M→L)) by a methionine residue, a CNBr cleavage site. The recombinant rOle(127M→L)-MPB fusion gene was cloned into the pET30a(+) vector and was expressed in E. coli (DE3) after IPTG induction under optimized conditions...
July 24, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28741463/effect-of-organic-solvents-on-porcine-pancreatic-lipase-thermal-aggregation
#19
Mahsa Vaezzadeh, Marjan Sabbaghian, Parichehreh Yaghmaei, Azadeh Ebrahim-Habibi
Proteins tend to form inactive aggregates under harsh conditions used in industrial processes. Lipases are enzymes that hydrolyze triglycerides to glycerol and free fatty acids, but are able to catalyze various other transformations in the presence of organic solvents. Considering this potential, a study on lipases behavior at high temperature and in presence of organic solvents is appropriate. Heat-induced aggregation of porcine pancreatic lipase (PPL) was followed by UV-visible spectroscopy at 400 nm wavelength for 600 seconds, at the isoelectric point (pH 5, phosphate solution) and 50°C, and in presence or absence of various percentages of dimethyl sulfoxide (DMSO), propanol, isopropanol, acetone and trifluoroethanol (TFE)...
July 24, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28741462/development-of-a-solid-phase-array-assay-for-the-screening-of-galactose-oxidase-activity-and-for-fast-identification-of-inhibitors
#20
Martin J Weissenborn, Damien P Debecker, Bruno Linclau, Nicolas J Turner, Sabine Flitsch
Galactose oxidase catalyses the highly selective oxidation of terminal galactosides on a wide range of natural glycoconjugates and has found wide applications in biotechnology, particularly in biocatalysis. Here we show that the enzyme is able to oxidise solid gold-SAM surfaces functionalised with galactose. This array platform allows for the fast identification of galactose oxidase substrates and inhibitors from a library of deoxy-fluorinated sugars using MALDI-Tof spectrometry as a label -free read-out method...
July 24, 2017: Protein and Peptide Letters
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