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Protein and Peptide Letters

Manjunatha Ks Gowda, Anitha Peter, Mahantesha Bn Naika, P Niranjana, P Shamprasad
Biotic stress affects all organisms, including the silkworm (Bombyx mori L.) which also has a plethora of defense mechanisms against the infecting pathogens. The midgut plays an important role as a natural barrier and source of innate immunity. The present investigation was thus undertaken to identify an antiviral red fluorescent protein formed in the midgut of the silkworm Bombyx mori L. The digestive juice from fifth instar Kolar Gold silkworm larvae was collected and subjected to 40 % ammonium sulfate precipitation and purified by gel filtration chromatography (GFC)...
January 15, 2018: Protein and Peptide Letters
Agnieszka Szkudlarek, Mariusz Mogielnicki, Danuta Pentak, Anna Ploch, Malgorzata Maciazek-Jurczyk
The aim of the study was to evaluate the mechanism of interaction between metoprolol (MTP) and human serum albumin (HSA) using 1H NMR technique. The analysis of the drug-albumin interaction was based on the observed chemical shifts in combination with correlation times, 2D NOESY 1H NMR spectra and association constants and allowed us to observe the interactions between the aromatic rings of the drug and the amino acids of the protein. The binding of the drug, both in the hydrophobic subdomain and on the surface of the albumin, increases in the presence of fatty acids (FAs)...
January 15, 2018: Protein and Peptide Letters
Sepideh Shahbazi, Azam Bolhassani, Arash Arashkia, Esmaeil Sadroddiny
Immunotherapies targeting the human papillomavirus (HPV) oncogenic proteins, E6 and E7, are effective to treat HPV-associated cervical malignancies. In this study, two types of the fusion E7-GFP proteins (i.e., with or without linker) were expressed in different E. coli strains. Then, the efficiency of GFP and E7-GFP transfection was compared with FITC-antibody protein control using TurboFect reagent in the HEK-293T cell line. Our data indicated that both E7-GFP fusion proteins were efficiently produced in M15 E...
January 15, 2018: Protein and Peptide Letters
Phanindra Babu Kasi, Marta Kotorman, Attila Borics, Beata Gabriella Hervay, Kinga Molnar, Lajos Laszlo
People have used ginseng as a traditional herbal medicine for over 2000 years, mainly for its protective effect against neurological disorders. In vitro studies were performed to study the formation of amyloid-like fibrils by the well-known protease trypsin as a model protein in 60% ethanol at pH 7.0. Possible inhibitory effects of ginseng extracts on the formation of amyloid-like fibrils was examined. The aggregation of trypsin was followed via turbidity measurements, aggregation kinetics, the Congo red (CR) binding assay, electronic circular dichroism (ECD) spectroscopy, size exclusion chromatography, and transmission electron microscopy (TEM) in the presence or absence of Panax ginseng extracts, as well as other, previously studied inhibitors of natural origin...
December 29, 2017: Protein and Peptide Letters
Srabani Karmakar, Kali P Das
α-Crystallin, the major eye lens protein in mammals is known to behave as a molecular chaperone. It is a member of the small heat shock protein family and is known to be linked with aging and cataract. Copper homeostasis, oxidative stress and interaction of Cu+2+ with α-crystallin are related to aging related neurodegenerative diseases. In order to understand the role of such interaction, we have studied the chaperone function and stability of α-crystallin by spectroscopic methods and detected the binding of Cu2+ by MALDI TOF mass spectrometry...
December 29, 2017: Protein and Peptide Letters
Kazutoshi Iijima, Mineo Hashizume
Various types of proteins play important roles in the biomineralization of hydroxyapatite (HAp, Ca10(PO4)6(OH)2). The resulting organic-HAp nanohybrids have highly-organized hierarchical structures that show unique morphological, structural, and mechanical properties. A clearer understanding of HAp mineralization mechanisms will provide novel design and fabrication concepts for functional hybrid materials. The roles of proteins in HAp mineralization in vivo were briefly explained in the first part of this review...
December 20, 2017: Protein and Peptide Letters
Kenji Usui, Shin-Ichiro Yokota, Makoto Ozaki, Shungo Sakashita, Takahito Imai, Kin-Ya Tomizaki
It was previously shown that the core sequence (the 9 C-terminal residues) of calcification-associated peptide (CAP-1), isolated from the exoskeleton of the red swamp crayfish, controls calcium carbonate precipitation with chitin, and replacement of the N-terminal serine phosphate with serine alters the precipitation ability of the peptide. In this study, we attempt to evaluate in detail the effect on calcium carbonate mineralization of phosphorylation, dephosphorylation, acetylation and deactylation of the N-terminus of the core sequence without chitin...
December 20, 2017: Protein and Peptide Letters
Hiroshi Tsutsumi, Hisakazu Mihara, Tomoko Shirai, Hiroyuki Ohkusa
Designed glycopeptides were conjugated with gold nanoparticles (GNPs) to produce optical probes for lectin detection and imaging on cell surface. Galactose was selected as a sugar unit for the glycopeptides. RCA120, a galactose binding lectin, was detected by the absorption change of GNPs with the galactose-modified peptides due to the aggregation with RCA120. The aggregation activity of GNPs for RCA120 depended on amino acid residues around the galactose unit of glycopeptides. In addition, a galactose binding protein on the surface of HepG2 cells was selectively visualized using GNPs with the galactose-modified peptides...
December 18, 2017: Protein and Peptide Letters
Amir Syahir, Kotaro Kajikawa, Hisakazu Mihara
Direct bio-monitoring essentially involves optical means since the photon has insignificant effects over biomolecules. Over the years, the laser induced surface plasmon resonance method with various modifications as well as versatile localized plasmon excited by incoherent light have facilitated in recording many nanobiological activities. Yet monitoring interactions of small molecules including drugs requires signal amplification and improvement on signal-to-noise ratio. This paper focused on how the refractive index based nanobio-sensoring gold platform can produce more efficient, adaptable and more practical detection techniques to observe molecular interactions at a high degree of sensitivity...
December 13, 2017: Protein and Peptide Letters
Nur Khaliesah Jamadon, Nurfatini Busairi, Amir Syahir
Mercury (II) ion is among the most common pollutants with the ability to affect the environment. The implications from their elevation in the environment are mainly due to the industrialisation and urbanisation processes. Current determination of heavy metal ions primarily depends on sophisticated instruments for heavy metal ions monitoring. A point-of-care detection method was developed using inhibitive enzymatic reaction monitored through a smartphone. Horseradish peroxidase converted 4-aminoantipyrene into a red coloured product visible with the naked eye...
December 13, 2017: Protein and Peptide Letters
Makoto Ozaki, Shungo Sakashita, Yoshio Hamada, Kenji Usui
Silica-based nanomaterials have been extensively investigated for many years because of their potential application in diverse technologies. The fabrication of such nanomaterials using existing strategies is generally inadequate. One approach to addressing this inadequacy is to mimic biological systems such as biomineralization systems. Considerable progress has been made over the past decade in understanding the molecular mechanisms underpinning biosilicification and the biomimetic synthesis of patterned nanosilica...
December 13, 2017: Protein and Peptide Letters
Toshiki Sawada, Takeshi Serizawa
M13 phages, a type of filamentous virus, have recently been utilized as components for developing novel functional soft materials in various fields. Herein, we demonstrated the self-healing capability of hybrid hydrogels composed of antigen peptide-displaying M13 phages and antibody-immobilized gold nanoparticles (GNPs). The addition of small amounts of buffer solutions to the rupture points of the hybrid hydrogels followed by incubation resulted in self-healing, possibly due to specific interactions between the components...
December 13, 2017: Protein and Peptide Letters
Jose Isagani B Janairo, Tatsuya Sakaguchi, Kenta Mine, Rui Kamada, Kazuyasu Sakaguchi
Properties and functions of inorganic nanomaterials are highly dependent on their size and shape. The peptide is principally responsible for the regulation of the growth and morphology of the inorganic nanostructure during peptide-mediated biomineralization. The 3D properties of the biomineralization peptide is a crucial factor in which the success of creating functional nanomaterials through biomineralization depends on. However, given the relatively short sequence of most biomineralization peptides, controlling the multivalency and spatial orientation of the peptide can be a challenging endeavor...
December 13, 2017: Protein and Peptide Letters
Kin-Ya Tomizaki, Yuichi Yamaguchi, Naoyuki Tsukamoto, Takahito Imai
Gold nanoparticles are promising nanomaterials for catalytic reactions, sensing/imaging systems, photonic/plasmonic devices, and electronics because of their unique physical and chemical properties. To date, significant catalytic activities of gold nanoparticles have been reported for reactions such as carbon monooxide oxidation and 4-nitrophenol reduction, and diverse gold nanoparticle morphologies such as nanospheres, wires, rods, and cubes have been achieved using a variety of capping/stabilizing organic molecules...
December 13, 2017: Protein and Peptide Letters
Kin-Ya Tomizaki, Ryuuichi Nanbu, Makoto Kasuga, Takahito Imai
Gold nanocrystals hold promise for use as catalysts and in photonic/plasmonic devices, electronics, sensing/imaging systems, drug delivery systems, and photothermal therapies due to their unique physical, chemical, and biocompatible properties. Various organic templates have been used to control of the size, shape, structure, surface modification and topology of gold nanocrystals. The synthesis of gold nanorods currently requires a cytotoxic surfactant to control morphology. To control the shape of gold nanocrystals, we previously demonstrated the de novo design and synthesis of a b-sheet-forming nonapeptide (RU006: Ac-AIAKAXKIA-NH2, X = L-2-naphthylalanine, Nal)...
December 13, 2017: Protein and Peptide Letters
Hikaru Nakazawa, Yasuko Seta, Tatsuya Hirose, Yoshitake Masuda, Mitsuo Umetsu
Recent advances in biotechnology now enable rapid identification of peptides that bind to inorganic materials. Here, we used a phage-display library to identify six peptides that bound to a tin oxide (SnO2) nanosheets. Characterization of these peptides resulted in identification of a peptide with a higher binding affinity for a SnO2 nanosheet than for spherical nanoparticles.
December 5, 2017: Protein and Peptide Letters
Kodai Otsubo, Takashi Yoneda, Atsushi Kaneko, Seiya Yagi, Kazuhiro Furukawa, Yoshiro Chuman
Protein phosphorylation is strictly regulated by protein kinases and protein phosphatases, and disordered regulation of protein phosphorylation often causes serious diseases, such as cancer. Protein phosphatases are divided into two major groups: tyrosine (Tyr) phosphatases and serine/threonine (Ser/Thr) phosphatases. Substrate trapping mutants are frequently used to characterize Tyr phosphatases and identify their substrates; however, a rapid and simple method to identify substrates for Ser/Thr phosphatases has yet to be developed...
December 5, 2017: Protein and Peptide Letters
Panchika Prangkio, Sirikran Juntapremjit, Melanie Koehler, Peter Hinterdorfer, Chanan Angsuthanasombat
Adenylate cyclase (CyaA) is one of the major virulence factors of Bordetella pertussis that plays a key role in whooping cough pathogenesis. A putative transmembrane helical hairpin (α2-loop-α3), encompassing residues 529-594 of CyaA hemolysin (CyaA-Hly) domain, was previously proposed to be crucially involved in hemolytic activity against target erythrocytes. Here, to gain more insight into membrane permeabilization of this toxin, synthetic peptides corresponding to this critical helical component, i.e. α2 (W528-G550), α3 (G568-R594) and α2-loop-α3 (W528-R594), were examined on various membrane models in comparison with the purified 130-kDa CyaA-Hly...
December 1, 2017: Protein and Peptide Letters
Mozafar Mohammadi, Peyman Bemani, Ali Hakakian
The most common recombinant antibody format is the single chain fragment variable (scFv) which it contains the complete antigen-binding domains of an intact antibody. ScFv fragments have found vast medical and non-medical applications. Several approaches have been employed to increase the affinity, avidity and structural stability related to these antibody fragments. Most approaches related to scFv improvement have been included in this review.
November 29, 2017: Protein and Peptide Letters
Muhammad Ijaz, Muhammad Shahbaz, Wenjie Jiang, Abdel Hamid Fathy, Effat Un Nesa, Wang Feng Shan
Growth factor receptor-bound protein 2 (Grb2) is a 25 kDa adaptor protein, which was originally discovered to accomplish basic cellular events such as cell growth, cell proliferation, and metabolism. However, recent studies evidenced that Grb2 was largely involved in multiple tumor malignancies. The mature Grb2 is a 217 amino acid sequence, which consists of one Src homology 2 (SH2) domain flanked by two Src homology 3 (SH3) domains. Using these binding motives, the ubiquitously expressed Grb2 acts as an intermediate between cell-surface activated receptors and downstream targets...
November 23, 2017: Protein and Peptide Letters
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