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Protein and Peptide Letters

Nan Lin, Qing Liu, Xiaoyu Wang, Jianmei Ma, Yuyuan Li
: Backgroud: Neuromyelitis optica (NMO) is an autoimmune inflammatory disorder, which is characterized by severe attacks of optic neuritis and myelitis. Antibodies (Ab) to aquaporin-4 (AQP4) (or NMO-IgG) as a serological biomarker of NMO have been widespread used. Nevertheless, some NMO patients remain seronegative for AQP4-Ab and/or have no detected optic nerve involvement. In addition, no consensus exists on the association between AQP4-Ab serostatus and visual outcome in NMO. To drive a more precise estimate of this postulated relationship, a meta-analysis was performed based on existing relevant studies...
January 10, 2017: Protein and Peptide Letters
Dominic Agyei, Ishtiaq Ahmed, Zain Akram, Hafiz M N Iqbal, Michael Kobina Danquah
Bioactive proteins and peptides are recognised as novel therapeutic molecules with varying biological properties for potential medical applications. Development of protein and peptide-based therapeutic products for human use is growing steadily as they continue to receive an increasing rate of approval by the United States Food and Drugs Administration (US FDA). In this short review, we describe the current status and methodologies involved in the synthesis of protein and peptide biopharmaceuticals with an emphasis on the drivers and restrains to their exploitation in the therapeutic products sector...
December 22, 2016: Protein and Peptide Letters
Lourdes Santiago-López, Aarón F González-Córdova, Adrián Hernández-Mendoza, Belinda Vallejo-Cordoba
In recent years, major developments in the field of inflammatory pathophysiology have clearly shown that arthritis, diabetes, intestinal bowel diseases, and obesity, which affect many people around the world, are essentially inflammatory in nature. Different anti-inflammatory drugs have been used to treat these conditions. Some people are able to take these drugs without difficulty, yet others experience negative side effects. Hence, the search for new, natural anti-inflammatory alternatives has rapidly increased in recent years...
December 22, 2016: Protein and Peptide Letters
Harvinder Kour Khera, Susheel Kumar Singh, Rafia Mir, Vikram Bhardwaj, Subhash Singh
Chorismate synthase (Cs) catalyzes the last step of Shikimate pathway involving a unique biochemical reaction of anti-1,4 elimination of 3-phosphate group and the C-(6proR) hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) leading to the formation of chorismate, which is the common precursor for aromatic amino acid, ubiquinone, and folate biosynthesis in plants and several bacterial, fungal, and parasitic pathogens. Absence of Shikimate pathway in the vertebrate host, make Cs an appealing target for drug discovery against these pathogens...
December 22, 2016: Protein and Peptide Letters
Somayeh Talebi, Azam Bolhassani, Talat Mokhtari Azad, Arash Arashkia, Mohammad Hossein Modaresi
Up to now, different protein vaccine modalities against human papillomavirus (HPV) have been designed to control cervical cancer. The important issue is to increase their immunogenicity using appropriate adjuvants. Among heat shock proteins (HSPs), glycoprotein 96 (Gp96) and its N-terminal region (NT-gp96) have attracted a specific interest in stimulation of antigen-specific immune responses in vivo. Furthermore, the potency of high mobility group box 1 (HMGB1) protein and its fragment (Hp91) was reported to enhance the desired immune responses against various disorders...
December 20, 2016: Protein and Peptide Letters
Joanna Stadnik, Paulina Kęska
The aim of this study was to evaluate the antimicrobial activity of meat protein-derived peptides against selected Gram-positive and Gram-negative bacteria. The in silico and in vitro approach was combined to determine the potency of antimicrobial peptides derived from pig (Sus scrofa) and cow (Bos taurus) proteins. The in silico studies consisted of an analysis of the amino acid composition of peptides obtained from the CAMPR3 database, their molecular weight and other physicochemical properties (isoelectric point, molar extinction coefficient, instability index, aliphatic index, hydropathy index and net charge)...
December 20, 2016: Protein and Peptide Letters
Indira P Sarethy
Bioactive peptides from plants are an underexplored domain in the field of proteomics and peptidomics. Recent evidences of plant peptides being more than chemical signals and indeed, functioning in the responses of plants to diverse environmental cues such as stress and infection by pathogens, suggests their potentially wide applicability in agrochemical and pharmaceutical industries. With cutting-edge technologies available, studies on plant peptides still lag behind those from food or microorganisms. The current mini-review provides an overview of the major bioactivity documented of plant-based peptides...
December 20, 2016: Protein and Peptide Letters
Tsogbadrakh Mishig-Ochir, Davaadulam Gombosuren, Altanchimeg Jigjid, Badamkhatan Tuguldur, Galbadrakh Chuluunbaatar, Enerelt Urnukhsaikhan, Chinar Pathak, Bong-Jin Lee
Naturally occurring antimicrobial peptides important for innate immunity are widely studied for their antimicrobial and anticancer activity. The primary target of these AMPs is believed to be the bacterial cytoplasmic membrane. However, the interaction between cytoplasmic membrane and the antimicrobial peptides remains poorly understood. Therefore to focus on the target membrane composition that is required by AMPs to interact with membranes, we have examined the interaction of the antimicrobial and anticancer active 11-residue GA-K4 (FLKWLFKWAKK) peptide with model and intact cell membranes...
December 16, 2016: Protein and Peptide Letters
Jad Abbass, Jean-Christophe Nebel
Protein structure prediction is still considered a main challenge in computational biology. The biannual international competition, Critical Assessment of protein Structure Prediction (CASP), has shown in its eleventh version that free modelling targets' predictions are still beyond reliable accuracy, therefore, much efforts should be done for ab initio methods. Arguably, Rosetta is considered as the most competitive method when it comes to targets with no homologues. Relying on fragments of length 9 and 3 from known structures, Rosetta creates putative structures by assembling candidate fragments...
December 16, 2016: Protein and Peptide Letters
Selvaraj Samuel, Harihar Balasubramanian
Predicting the unfolding rates of proteins remains complicated due to the intricacy present in the unfolding pathway of proteins and further it was observed that the experimental unfolding data were less while compared to folding kinetics. The aim of our present work is to show the variation in long-range contacts observed in various sequence separation bins belonging to all-α, all-β and mixed structural classes of 52 two-state proteins. In this work linear regression technique have been used and regression equations were developed using long-range contacts observed from various sequence separation bins...
December 16, 2016: Protein and Peptide Letters
Zhenglin Gong, Yi Feng, Bo Yu, Ruizhi Zhang, Ping Zhang, Menglong Cao, Haoliang Liu, Tiantian Yang, Rui Luo
Checkpoint kinase 2 (Chk2) is a significant mediator of diverse responses to DNA damage. The present study was aimed to identify possible interactive proteins of Chk2 and try to clarify the underlying mechanism regarding Chk2 chromatin loading and its phosphorylation to DNA damage response in oral squamous cell carcinoma (OSCC). Differently tagged Chk2 and minichromosome maintenance (MCM) complex (MCM2, MCM3, MCM5, and MCM6) were overexpressed into SCC-4 cells. After 48 h of transfection cell fractionation was performed to localize proteins...
December 12, 2016: Protein and Peptide Letters
Osama Chahrour, John Malone
Recent advances in inductively coupled plasma mass spectrometry (ICP-MS) hyphenated to different separation techniques have promoted it as a valuable tool in protein/peptide quantification. These emerging ICP-MS applications allow absolute quantification by measuring specific elemental responses. One approach quantifies elements already present in the structure of the target peptide (e.g. phosphorus and sulphur) as natural tags. Quantification of these natural tags allows the elucidation of the degree of protein phosphorylation in addition to absolute protein quantification...
December 12, 2016: Protein and Peptide Letters
K M Sharnabai, M Krishnamurthy, N R Sagar, L Santhosh, Sureshbabu V Vommina
An efficient oxidative chlorination of thiols to Nα-protected amino alkyl sulfonyl azides is delineated. The reaction involves in situ generation of sulfonyl chloride employing N-chlorosuccinimide and tetrabutylammonium chloride-water in acetonitrile, followed by the reaction with sodium azide. The protocol is simple, straight forward, mild and high yielding. Amino acids with simple as well as bifunctional side chains were used to obtain Nα-protected amino alkyl sulfonyl azides. Further, sulfonyl azides were utilized to synthesize unnatural amino acids via Cu(OAc)2...
November 30, 2016: Protein and Peptide Letters
Reza Fotovat, Mehdi Alikhani, Mostafa Valizadeh, Mehdi Mirzaei, Ghasem Hossein Salekdeh
Plants reproductive phase, when grain yield and consequently farmers' investment is most in jeopardy, is considered as the most sensitive stage to drought stress. In this study, we aimed to explore the proteomic response of wheat anther at meiosis stage in a drought tolerant, Darab, and susceptible, Shiraz, wheat genotypes. Wheat plants were exposed to drought stress at meiosis stage for four days under controlled environmental conditions. Then, anthers from both genotypes were sampled, and their proteomes were examined via quantitative proteomics analysis...
November 30, 2016: Protein and Peptide Letters
Xianyang Li, Xiaoting Hua, Liang Song, Qingyou Xia, Fei Wang
Src is a non-receptor protein tyrosine kinase ubiquitously expressed in animals. It is involved in various cellular processes, including the innate immune response in mammals. However, less is known about the function of insect Src. Here we presented a homologue of Src in silkworm (Bombyx mori), named as BmSrc by phylogenetic analysis, homologous comparison and domain prediction. BmSrc contains the conserved phosphorylation residues and possesses tyrosine kinase activity. The expression pattern of BmSrc mRNA was specific in stages and tissues...
November 29, 2016: Protein and Peptide Letters
Yinliang Ma, Guohui Bai, Yaqi Cui, Jing Zhao, Zenglin Yuan, Xiuhua Liu
Peptidoglycan (PG) is an essential component of the cell wall, and undergoes reconstruction by various PG hydrolases during cell growth, development and division. The murein- tripeptide (Mtp) amidase MpaA belongs to PG hydrolase family and is responsible for cleaving the γ-D-Glu-meso-Dap amide bond in the Mtp released during PG turnover. The current paper reports the crystal structure of MpaA from Escherichia coli (E. coli) O157 at 2.6 Å resolution. The asymmetric unit consists of two protein molecules and each monomer represents the common α/β fold of metallo-carboxypeptidases (MCP)...
November 28, 2016: Protein and Peptide Letters
Heba El Gamal, Shankar Munusamy
Diabetic nephropathy (DN) is one of the most serious microvascular complications of diabetes mellitus and the leading cause of end stage renal disease. One of the key pathways activated in DN is the polyol pathway, in which glucose is converted to sorbitol (a relatively non-metabolizable sugar) by the enzyme aldose reductase (AR). Shunting of glucose into this pathway causes disruption to glucose metabolism and subsequently damages the tissues via increased oxidative stress, protein kinase c activation and production of advanced glycation end products (AGE) in the kidney...
November 28, 2016: Protein and Peptide Letters
Anna Walaszczyk, Monika Pietrowska, Anna Wojakowska, Agata Abramowicz, Aleksandra Chmura, Barbara Masłyk, Paweł Rodziewicz, Joanna Polańska, Katarzyna Behrendt, Elżbieta Nowicka, Rafał Tarnawski, Piotr Widłak
Adjuvant chemo- and/or radiotherapy is applied in majority of patients treated because of early stage breast cancer even though only a small fraction of them is at high risk of metastasis or recurrence. Hence, knowledge of biomarkers associated with risk of disease progression might help planning of an optimal therapy, and to protect large group of patients from toxicity of unnecessary treatment. Here we aimed to characterize serum proteome of patients diagnosed with early stage breast cancer who either showed no evidence of disease five years after the end of therapy or suffered from metastasis, relapse or second cancer during corresponding follow-up...
November 28, 2016: Protein and Peptide Letters
Lucía Brown, Esteban Vera Pingitore, Fernanda Mozzi, Lucila Saavedra, Josefina M Villegas, Elvira M Hebert
There is a growing interest in the incorporation of functional foods in the daily diet to achieve health promotion and disease risk reduction. Numerous studies have focused on the production of biologically active peptides as nutraceuticals and functional food ingredients due to their health benefits. These short peptides, displaying antihypertensive, antioxidant, mineral binding, immunomodulatory and antimicrobial activities are hidden in a latent state within the primary sequences of food proteins requiring enzymatic proteolysis for their release...
November 23, 2016: Protein and Peptide Letters
Suvra Roy, Vikash Kumar, Vikas Kumar, B K Behera
The acute phase proteins are biochemically and functionally unrelated protein predominantly synthesized in the liver. The local inflammatory cells i.e. macrophages and neutrophils secretes various cytokines like IL-1, IL-6, IL-8 (interleukins) and TNF-α into bloodstream in response to injury and tissue damage, which stimulate hepatocytes to produce protein and release them into the circulation; these proteins are called as acute phase protein (e.g. C-reactive protein (CRP), serum amyloid A (SAA), metal binding protein, lysozyme, lectin, etc...
November 21, 2016: Protein and Peptide Letters
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