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Protein and Peptide Letters

Deshuai Lou, Jun Tan, Liancai Zhu, Shunlin Ji, Shijin Tang, Kaiyi Yao, Jingxuan Han, Bochu Wang
Enhancing thermostability of the 7α-Hydroxysteroid dehydrogenases (7α-HSDHs) is beneficial to its industrial application broadly. Based on unfolding free energy changes (ΔΔG) prediction seven mutations of Clostridium absonum (CA) 7α-HSDH were selected and experimentally verified, and these mutants fitted three-state denaturation model well, among which S22L located in the αA possessed the greatest TmN→I increase (>8 °C). Mutants P124L, L197E, N171L and Y259E also became more stable than wild type CA 7α-HSDH with different ranges...
November 12, 2017: Protein and Peptide Letters
Yunxiao Sun, Zhen Peng, Libiao Zhang, Huaqian Wang, Xiangyang He, Xingwen Peng, Qin Zhang, Hui Liu, Junhua Rao, Haifeng Wang, Jie Wu
Experimental autoimmune encephalomyelitis (EAE) induced by self-myelin antigen is a widely used multiple sclerosis (MS) model for preclinical studies of new therapeutics and potential pathogenesis. By comparison with rodent EAE models, EAE models in primates are more similar to MS. Some groups have developed EAE models in primates by using common marmoset (Callithrix jacchus). However, this model has some limitations. EAE in cynomolgus monkey (Macaque fasciculrais) could overcome these limitations. In this study, EAE was induced in cynomolgus monkey by immunizing with the recombinant human myelin oligodendrocyte glycoprotein extracellular domain (1-125) (rhMOG1-125) and a synthetic peptide, representing peptide 34-56 of human myelin oligodendrocyte glycoprotein (MOG34-56) in complete Freund's adjuvant (CFA) and in combination with intravenous injection of pertussis toxin, and subsequent booster immunizations with the same dose of antigen in incomplete Freund's adjuvant (IFA) until the animals developed clinically significant EAE (score≥2)...
November 9, 2017: Protein and Peptide Letters
Man Man Zong, Guang Fang Zhou, Yang Zheng, Yuan Nan Yu, Chuan Jie Zhou, Xiu Li Feng, Rui Bin Cao, Pu Yan Chen, Mei Yang
The bursa of Fabricius (BF) is an acknowledged central immune organ, and is important to the B cell differentiation. Bursal hexapeptide (BHP) is the recently reported bursal-derived peptide, while its inducing function on immune response is uncertain. In this study, mice were immunized with Japanese encephalitis virus (JEV) vaccine and BHP from 0.01 mg/mL to 0.25 mg/mL to detect antibody response and cellular immune response, respectively. The results showed that BHP co-immunization with JEV vaccine generated significant increased antibody levels, neutralizing antibody titers and spleen lymphocyte viability, compared to that of vaccine control...
October 30, 2017: Protein and Peptide Letters
Luciana Malavolta, Ricardo Lobato Chianarelli, Danielle Vieira Sobral, Clovis Ryuichi Nakaie
This work developed an approach to first examine chain association propensities of several amyloidogenic peptides: SNNFGAILSS from the islet amyloid polypeptide (coded IAPP), NAGDVAFV from the protein responsible for corneal amyloidosis (coded Lactoferrin), and (1-42) β-amyloid (coded Amyloid). The fluorescence and light scattering experiments results indicated that Amyloid revealed a surprising reduction in the aggregation process as a function of time (decrease of about 20-30% in 3 days) through both methods...
October 30, 2017: Protein and Peptide Letters
Xiaoguo Ji, Jihong Huang, Ming Hui, Yaqi Zhang, Yi Zhao
Wheat germ, one of the byproducts of flour milling, contains many physiologically active components. In this study, the immunoregulatory effects of wheat germ globulin (WGG) on immunosuppressed mice induced by cyclophosphamide were investigated, and the immunological activity of this molecule was explored. The main components, functions and metabolic signaling pathways of this protein were analyzed by a combination of proteomic technology and bioinformatics. The structure of WGG was predicted with the Phyre2 tool...
October 26, 2017: Protein and Peptide Letters
Fotini Pouliou, Fereniki Perperopoulou, Nikolaos E Labrou
In the present work, we report the comparative analysis of the structural and functional features of two isoenzymes (GmGSTU5-5 and GmGSTU8-8) of the glutathione transferase (GST) family from Glycine max. Full-length cDNA clones of GmGSTU5-5 and GmGSTU8-8 were derived by RT-PCR of RNA isolated from Glycine max leaves and were cloned into a T7 expression vector. Τhe recombinant enzymes were expressed in E. coli and purified by affinity chromatography. Analysis of substrate specificity using a range of thiol substrates and xenobiotic compounds suggested that both isoenzymes display a range of broad and overlapping specificities and are capable of detoxifying major stress-induced toxic products...
October 26, 2017: Protein and Peptide Letters
Yi Wu, Min Yu, Zujun Sun, Weihua Hou, Yuxiong Wang, Qingyun Yuan, Wei Mo
Bispecific antibodies, BsAbs, are molecules with the ability to bind to two different epitopes on the same or different antigens. The goal of this study was to create a novel bispecific antibody targeting both cellular-mesenchymal to epithelial transition factor(c-MET) and programmed death-1(PD-1) as an anti-cancer therapeutic candidate. Upon binding to the c-MET antigen on a tumor cell and the PD-1 antigen on a T cell, a BsAb can redirect T cells for tumor killing. Based on the original sequences of PD-1 and c-MET mAbs, a BsAb gene was designed, and cloned into the pCEP4 vector...
October 17, 2017: Protein and Peptide Letters
Pooneh Rahimi, Rouhollah Vahabpour, Fatemeh Motevalli, Mohammad Shayestehpour, Mohammad Reza Amiran, Shaghayegh Yazdani, Sara Bagheri
HCV E2 glycoprotein which mediates attachment to target cells via cell surface receptors is one of the most attractive proteins for designing an effective vaccine. The hydrophobic carboxyl-terminal (C-terminal) region of this protein act as the membrane anchors. A truncated form of E2 protein from HCV genotype 2a (JFH1) was amplified by PCR using specific primers and cloned into a pET28a(+) vector. E-coli DH5α strain was transformed by this recombinant vector through heat shock method. Truncated E2 protein was expressed in BL21 (DE3), and its specific antibody was detected in serum samples from HCV infected patients...
October 9, 2017: Protein and Peptide Letters
Divya Rupa Venkata Pandala, Jogeswar Gadi, Kiranmayi Patnala
Zinc transporter (tzn-1) of Neurospora crassa plays a crucial role in conidiation pathway, as its removal results in aconidiation which was reported in our earlier studies. Present study depicts the role of tzn-1 in conidiation process, by comparing knockout (KO) mutants zinc transporter KO (Δtzn-1) and aconidiating gene KO (Δacon-3) with wild oak ridge (OR) 74 'A' strain by 'Proteo-genomic' approach. Firstly, nine commonly overexpressed protein spots from the two sets (Δtzn-1 to wild and Δacon-3 to wild) were analyzed by combining 2-Dimensional gel electrophoresis (2DE) with Matrix Associated Laser Desorption/Ionization mass spectrometry -Peptide Mass Fingerprint (MALDI-PMF)...
September 19, 2017: Protein and Peptide Letters
Aabgeena Naeem, Taqi Khan, Tauqir Alam, Qayyum Husain
Concanavalin A (Con A) immobilized on the hexagonal zinc oxide (ZnO) nanoparticles, using various techniques like atomic force microscopy, scanning electron microscopy and X-ray diffraction showed changes in morphology of Con A and ZnO nanoparticles. FT-IR spectroscopy confirmed the linking of Con A amino groups with ZnO nanoparticles. Con A immobilized nanoparticles in contrast to native lectin showed visible changes in hemagglutination activity as confirmed by pH dependence studies using fluorimetry. The Con A coated ZnO nanoparticles did not induce any significant damage to whole cell DNA as revealed from comet assay or plasmid DNA...
September 19, 2017: Protein and Peptide Letters
Cuijuan Yuan, Yangchen Lin, Yushu Ma, Dongzhi Wei
The histidine kinase response regulator hybrid protein was identified as a member of two-component signaling systems in Gluconobacter oxydans by gene annotation, but the function of this protein remains unknown. In this research, its specific interaction with a 2-keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase was screened by yeast two-hybrid assay. The interaction between the two proteins was further identified by GST pull-down assay and bimolecular fluorescence complementation assay in vitro and in vivo, respectively...
September 19, 2017: Protein and Peptide Letters
Baoliang Ma, Fan Zhang, Yujie Liu, Jinbing Xie, Xiaofei Wang
Investigating how small molecules affect the amyloid aggregation of proteins is of importance for preventing and treating various neurodegenerative diseases. Here, the influences of resveratrol on the conformation and amyloid aggregation of β-lactoglobulin were studied. The results showed that resveratrol altered the conformation and increased the hydrophobicity of β-LG. In addition, it was observed that the increase in resveratrol concentration resulted in shorter lag time and faster growth of fibrils. However, we also observed that the yield of amyloid fibrils was significantly reduced by the addition of resveratrol...
September 18, 2017: Protein and Peptide Letters
Azadeh Hekmat, Zahra Hajebrahimi, Amir Motamedzade
Nowadays, the biological effects of microgravity have been the subject of various experimental researches. The purpose of this study was to investigate the probable biological effects of microgravity on the human serum albumin (HSA) structure after 3 and 24 h exposure via various spectroscopic instruments. The UV-Visible, near-UV-CD and intrinsic fluorescence spectroscopy represented that microgravity can remarkably change the tertiary structure of HSA. Additionally, the ANS affinity for HSA incremented when the protein was exposed to simulate microgravity compared to unexposed HSA, which may possibly have appeared attributable to expansion of the structure of simulated HSA...
September 17, 2017: Protein and Peptide Letters
Adriana Patarroyo Vargas, Yaremis Meriño Cabrera, José Cola Zanuncio, Francelina Rocha, Wellington Garcia Campos, Maria Goreti de Almeida Oliveira
The kinetics of Anticarsia gemmatalis Hübner gut serine proteases with tripeptidyl substrates, and the enzymatic inhibition model using synthetic peptides Gor 3, Gor 4, and Gor 5 are described. The enzymes were purified on p-aminobenzamidine agarose affinity column in an FPLC system using electrophoresis with 12.5% polyacrylamide gel. Michaelis-Menten constants and the inhibition model were determined according to the Dixon methodology and Lineweaver-Burk's double reciprocal. The KM values and catalytic constants of peptide substrates show that A...
September 17, 2017: Protein and Peptide Letters
Sarbashri Bank, Pradipta Jana, G V Girish, Asru Sinha, Smarajit Maiti
Organic "nitro" compounds such as nitroglycerine, isosorbate nitrate are useful in the control of chest pain in acute coronary syndrome. But the mechanism of it in pain regulation remains speculative. Here, increase of NO production was investigated by the possible regulation of constitutive nitric oxide synthase (cNOS) function from goat arterial endothelial cells. This protein was purified and sequence wise characterized as protein disulfide isomerase (PDI) in response to different nitro compounds. The NO generating protein was isolated from arterial endothelial cells and prepared to homogeneity...
September 11, 2017: Protein and Peptide Letters
Razvan T Radulescu
Three important aspects of tumor suppressor protein (TSP) function are highlighted here: anti-inflammatory activity, wound healing and storage. These features should foster both TSP understanding and drug design.
August 30, 2017: Protein and Peptide Letters
Ana Flavia Almeida-Santos, Lucas Miranda Kangussu, Maria Jose Campagnole-Santos
A large body of studies characterized the renal and cardiovascular effects of the peptides of the renin-angiotensin system (RAS). We now recognize that, in addition to angiotensin (Ang) II and Ang III, other peptides, such as, Ang-(1-7), Ang-(1-9), Ang IV and Alamandine can mediate the actions of RAS in different tissues, including the brain. The effects elicited by angiotensins in the brain are complex, site specific and dependent on the interaction with selective receptors, angiotensin type 1 receptor (AT1), AT2, Mas or MrgD, which present widespread distribution in the central nervous system...
August 22, 2017: Protein and Peptide Letters
Caihong Liu, Yurou Cao, Jihong Yang, Haobin Zhao, Kashif Rafiq Zahid, Jin Zhao, Chao Qi, Jinlin Liu
Identification of immunogenic antigens is an important step for the vaccine improvement of infectious diseases. Previous studies indicated that Actinobacillus pleuropneumoniae PalA is homologous to a Haemophilus influenzae protective antigen Hi-PAL (P6) protein. However, PalA protein adversely affects the Apx toxin-based subunit vaccine. In this study, we confirmed that recombinant PalA is immunogenic in animals. Mice immunized with bacterin and PalA (BacPal) showed significantly less antibodies against Apx toxins, relative to that of animals immunized with bacterin alone (Bac) before challenge...
August 22, 2017: Protein and Peptide Letters
Jin Zheng, Tian Yuan, Yan Dongmei, Li Dong, Zhu Xun
OBJECTIVE: In our previous study, we showed that Bacillus Calmette-Guerin (BCG)-activated macrophages have the ability to directly kill tumor cells. One of the main properties of these macrophages is the high expression of tripartite motif family protein 59 (TRIM59). This study was conducted to investigate the mechanism of BCG-induced TRIM59 expression on macrophages and to identify the subcellular localization of TRIM59. METHODS: TRIM59 expression and TNF-α secretion were compared in RAW264...
August 18, 2017: Protein and Peptide Letters
Abhijit Chakrabarti, Sushanta Debnath, Bikram Nath
Misfolding of proteins often leads to aggregation. Accumulation of diverse protein aggregates in various cells, tissue and organs is the hallmark of many diseases, such as Alzheimer's disease and Parkinson's disease. In this report, we present a novel method of characterization of protein aggregates, associated with differential toxicity with different size and composition in vitro using flow cytometry. Autofluorescence and scattering intensities could distinguish between amyloid and non-amyloid aggregates...
August 18, 2017: Protein and Peptide Letters
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