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Protein and Peptide Letters

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https://www.readbyqxmd.com/read/28425860/a-novel-recombinant-multi-epitope-vaccine-could-induce-specific-cytotoxic-t-lymphocyte-response-in-vitro-and-in-vivo
#1
Yahong Wu, Wenjie Zhai, Meng Sun, Zhe Zou, Xiuman Zhou, Guodong Li, Zhongyi Yan, Yuanming Qi, Yanfeng Gao
Cytotoxic T lymphocytes (CTLs) against various tumor antigens play an important role in elimination of tumor cells. To develop therapeutic vaccines that can induce specific immune response, we designed a novel recombinant multiepitope vaccine YL66 which consists of HLA-A2-restricted CTL epitopes from two widely expressed tumor antigen cyclooxygenase-2 and MAGE-4, linked with membrane permeable Tat-PTD and the universal T helper epitope. The DNA fragment of YL66 was cloned into pGEX-4T-2-YL66 and pcDNA3.1(+)-YL66, respectively...
April 19, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28425861/role-of-cysteine-residues-in-regulation-of-peptidyl-prolyl-cis-trans-isomerase-activity-of-wheat-cyclophilin-tacypa-1
#2
Prabhjeet Singh, Gundeep Kaur, Harpreet Singh, Kirandeep Kaur, Ashwani Pareek
Oxidative conditions result in inhibition of peptidyl-prolyl cis-trans isomerase (PPIase) activity of several cyclophilins. Thiol groups have been implicated in redox regulation of these proteins. In our previous study, we proposed that activity of wheat cyclophilin, TaCYPA-1, may be modulated through a novel dual mechanism of redox regulation. To further understand the regulation of PPIase activity of TaCYPA-1, we generated mutants of TaCYPA-1 by substituting cysteine residues at positions -40 and -122 with serine, and at -126 with proline...
April 17, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28413971/structure-of-c-terminal-domain-of-peptidyl-prolyl-cis-trans-isomerase-from-pseudomonas-syringae-pv-tomato-str-dc3000-at-1-6%C3%A3-resolution
#3
Yu Gao, Hong-Mei Zhang, Piwu Wang, Mei Li
Peptidyl-prolyl cis-trans isomerase (PPIase) catalyses the conversion between the cis and trans configurations of proline which switches the backbone conformation of peptides. We determined the crystal structure of the C-terminal domain of PPIase from Pseudomonas syringae pv. tomato DC3000 (PSPTO DC3000) at 1.6Å resolution. Structural comparison of PSPTO-PPIase with its homologues suggested that the successive arrangement of residues with small side-chain in loop 4 contributes to its substrate specificity...
April 13, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28412901/an-analysis-of-central-residues-between-ligand-bound-and-ligand-free-protein-structures-based-on-network-approach
#4
Amala Arumugam, Arnold Emerson Isacc
Depiction of protein structures as networks of interacting residues have enabled us to understand the structure and function of the protein. Previous investigations on closeness centrality have identified protein functional sites from three- dimensional structures. It is well recognized that ligand binding to a receptor protein induces a wide range of structural changes. An interesting question is, how the central residues are functioning during conformational changes triggered by the ligand binding? The aim of this study is to analyze at what extent the central residues are changing upon ligand binding to receptor proteins...
April 13, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28393686/impact-of-salt-concentration-and-ph-on-surface-charged-residues-controlling-protein-association-pathways-in-carboxylesterase-estgta2
#5
Jessica K Moisan, Fatma Meddeb-Mouelhi, David M Charbonneau, Marc Beauregard
Understanding the relationship between enzymatic stability and the amino acid sequence encoding such enzymes is of utmost importance. Here we thoroughly characterized the behavior of the carboxylesterase EstGtA2 from Geobacillus thermodenitrificans during thermal denaturation at different pH with various salt concentrations. EstGtA2 was characterized by circular dichroism regarding conformation and thermal stability, by dynamic light scattering for detection of association/aggregation, by enzymatic assays for activity, and by monitoring the impact of heat treatments on activity...
April 4, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28393685/immunoproteomics-approach-for-screening-vaccine-candidates-against-the-intestinal-botulism
#6
Arti Sharma, Sarkaraisamy Ponmariappam, Sarita Rani
Intestinal botulism is an infectious form of botulism in which disease results from ingesting spores, which is followed by spore germination and intraluminal production of botulinum neurotoxins over an extended period. Botulinum neurotoxin is produced by endospore forming bacteria called C. botulinum. The immunoproteomic is used to screen the cross reactive immunogenic proteins of C. botulinum type B using C. botulinum type B live spore antiserum. The whole cell proteins are separated by two dimensional gel electrophoresis and transferred to polyvinylidene difluoride membranes...
April 4, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28393684/generation-of-the-fluorescent-hmgb1-gfp-fusion-protein-in-insect-cells-and-evaluation-of-its-immunogenicity-in-two-mice-models
#7
Ali Anvar, Rouhollah Vahabpour, Iman Salahshourifar, Azam Bolhassani
High mobility group box 1 (HMGB1) is a highly conserved protein present in the nuclei and cytoplasm of cells which has an important role as a mediator of inflammation in the extracellular environment. HMGB1 was identified as an innate adjuvant that induces immune responses against soluble antigens in vivo. In the current study, we used a baculovirus expression system for insect cells that was based on expression of HMGB1 with target gene (GFP), and purified the recombinant HMGB1-GFP fusion protein. We then demonstrated whether immunogenicity of GFP changes in the presence or absence of recombinant HMGB1 acting as an adjuvant in C57BL/6 and BALB/c mice...
April 4, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28393683/identification-and-characterization-of-strychnine-binding-peptide-using-phage-display-screening
#8
Fang Zhang, Min Wang, Zheng Qiu, Xiao-Meng Wang, Chun-Lei Xu, Xia Zhang
Phage display has been proved in drug development as a high-throughput method for identifying specific cellular target of different drugs. However, phage display remains an intricate process for insoluble small chemicals because of the difficulty of presenting the molecules to the phages without occupying or destroying the very limited functional groups. In the present study, with Strychnine (Stry) selected as a model compound, we have developed an alternative in vitro bio-panning method against insoluble suspension...
April 4, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28294044/ca2-binding-and-conformational-switch-of-the-photoprotein-mnemiopsin
#9
Shima Tarahomi, Reza H Sajedi, Hossein Rahmani, Bijan Ranjbar, Majid Taghdir
Bioluminescence in Ca2+-binding photoproteins is an intramolecular reaction triggered by the addition of Ca2+. A comparative study has been done on Ca2+-depleted and Ca2+-loaded apo-mnemiopsin to understand the structural transition of the photoprotein by Ca2+ binding. Ca2+ is removed by TCA (trichloroacetic acid) precipitation to obtain Ca2+-depleted apo-mnemiopsin. UV-visible, CD and fluorescence spectroscopic studies demonstrate that the addition of Ca2+ is brought about by the overall structure of apo-mnemiopsin becomes more open in a concentration-dependent manner without significantly influencing the secondary structure and indicate that the Ca2+-depleted form of apo-mnemiopsin, in contrast to most other EF-hand calcium binding proteins, adopt a closed conformation when compared to the Ca2+-loaded form...
March 10, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28266265/effects-of-dietary-iron-modulation-on-gut-microbial-composition-and-function-in-monogastrics-a-review
#10
Guixin Qin, Rui Han, Bao Zhao, Hailong Jiang, Mohammed Hamdy Farouk, Chun Zhang, Dongsheng Che
Dietary iron is a crucial nutrient element for biological processes of both hosts and gut microbiota. Deficiency in dietary iron is a highly common disorder in the developing locations of the world and can be healed by oral iron administration or complementary iron diet. While the redundant iron that enters the gut lumen leads to negative effects, and modulates the gut microbial composition and function. Such modulation led to a significant effect on vital biological pathways of the host, including metabolic disease (obesity and type 2 diabetes), metabolites (SCFA, blood glucose and cholesterol), bile acid metabolism, endocrine, neural, and other well-being patterns...
March 5, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28260503/in-vivo-neutralization-of-botulinum-neurotoxin-serotype-e-using-rabbit-polyclonal-antibody-developed-against-bont-e-light-chain
#11
Sarita Sarita, Ponmariappan Sarkaraisamy, Arti Sharma, D V Kamboj, A K Jain
Clostridium botulinum is an obligate anaerobic, Gram positive bacterium that secretes extremely toxic substances known as botulinum neurotoxins (BoNTs) that cause serious paralytic illness called botulism. Based upon the serological properties, these neurotoxin have been classified into seven serotypes designated from A to G. Due to extreme toxicity of BoNTs, these neurotoxins have been designated as category A biowarfare agents. There is no commercial neutralizing antibody available for the treatment of botulism...
March 1, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28260502/selective-binding-baff-april-by-the-in-and-outside-conservative-region-of-bcma
#12
Chang Zheng, Xiaojuan Zhang, Zhen Zhao, Xiaofei Hao, Jing Wei, Jian Sun
B-cell activating factor (BAFF) and a proliferation-inducing ligand (APRIL) play vital roles in the pathogenesis of autoimmune diseases. B cell maturation antigen (BCMA), a receptor of both BAFF and APRIL shows higher affinity for APRIL than for BAFF. In this study, the conserved region of BCMA was divided into two segments BCMA1 (NEYFDSLLHACIPC) and BCMA2 (QLRCSSNTPPLT) with the aim to investigate their contribution to the ligands binding, BCMA1 contains the conservative DxL domain while BCMA2 is the segment outside the main functional area...
March 1, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28245756/diosmetin-induces-cell-apoptosis-by-regulating-cyp1a1-cyp1a2-due-to-p53-activation-in-hepg2-cells
#13
Bin Liu, Kaiqiao Jia, Yu Yang, Siyuan Hao, Caijie Lu, Fengming Xu, Dehui Feng, Runzhi Zhu
It was explored that CYP1 family of cytochromes P450 were over-expressed in several types of cancer. Our study aimed to characterize anti-proliferative activity and metabolism of the natural flavonoid diosmetin in the human hepatoma cell HepG2, expressing CYP1 family. Dios-induced cell apoptosis could be reversed due to p53 blockade and the cellular P53 and CYP1A1/CYP1A2 proteins levels were examined. P53 and CYP1A1/CYP1A2 proteins were up-regulated by Dios; when PFT-α was added into cells, the P53 levels were down-regulated accompanied with up-regulated CYP1A1/CYP1A2...
February 27, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28245755/towards-binding-mechanism-of-cu2-on-creatine-kinase-from-pelodiscus-sinensis-molecular-dynamics-simulation-integrating-inhibition-kinetics-study
#14
Yan Cai, Jinhyuk Lee, Wei Wang, Yong-Doo Park, Guo-Ying Qian
Cu2+ is well known to play important roles in living organisms having bifacial distinction: essential microelement that is necessary for a wide range of metabolic processes but hyper-accumulation of Cu2+ can be toxic. The physiological function of Cu2+ in ectothermic animals such as Pelodiscus sinensis (Chinese soft-shelled turtle) has not been elucidated. In this study, we elucidated effect of Cu2+ on the energy producing metabolic enzyme creatine kinase (CK), which might directly affect energy metabolism and homeostasis of P...
February 27, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28240162/effects-of-dietary-non-fiber-carbohydrates-on-composition-and-function-of-gut-microbiome-in-monogastrics-a-review
#15
Hailong Jiang, Dongsheng Che, Guixin Qin, Xiangjie Kong, Mohammed Hamdy Farouk
Non-fiber carbohydrates (NFC) have a crucial function on the gut health of monogastrics. This paper aims to review the relevant published materials on the influence of NFC's on the gut's microbial population and composition in monogastrics, and points out the areas of the required research. Total bacteria count and Lactobacillus sp. were decreased with an increase in composition of dietary NFC intake, as well as accompanied by a decrease in the short-chain fatty acids (SCFAs) levels. Consequently, some metabolites were affected by the accumulation of the bile acids, including molecules which control different gene expression levels, as regulators involved in glucose (FXR and TGR5) and fat metabolism (cholesterol)...
February 23, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28240161/dihydromyricetin-reduces-tgf-%C3%AE-via-p53-activation-dependent-mechanism-in-hepatocellular-carcinoma-hepg2-cells
#16
Xiaojie Huang, Tianming Lian, Xiaoqian Guan, Bin Liu, Zeqing Song, Jingjing Zhang, Shiting Bao, Runzhi Zhu
Natural antineoplastic drug development is crucial to treatment of clinical oncology. Dihydromyricetin, a bioactive flavonoid compound was extracted from the stems and leaves of Ampelopsis grossedentata. It exhibited anticancer activity and induced apoptosis in human hepatocellular carcinoma cells according to our previous studies. In this study, we demonstrated that DHM could significantly inhibit proliferation and induce apoptosis in HepG2 cells with MTT and Flow Cytometry methods. It is very interesting that we found DHM could regulate TGF-β signal pathway and which has a crosstalk with P53, Smad3 and P-Smad2/3 proteins...
February 23, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28240160/dietary-additive-probiotics-modulation-of-the-intestinal-microbiota
#17
Shenglan Hu, Li Wang, Zongyong Jiang
The importance of the intestinal microbiota of animals is widely acknowledged because of its vital role in the health of animals. There are complex communities of microbiota, which colonize the gastrointestinal tract. Intestinal microbiota are conductive to animal health and the development of the host immune system. Probiotics are commonly used dietary additives where they provide the host with many beneficial functions, such as modulating intestinal homeostasis and promoting gut health. These beneficial effects of probiotics may accrue from the inhibiting the growth of pathogenic bacteria and promoting the growth of beneficial flora in the gastrointestinal tract...
February 23, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28240159/supplementation-of-milky-flavors-improves-the-reproductive-performance-and-gut-function-using-sow-model
#18
Long He, Jianjun Zang, Ping Liu, Peixin Fan, Peixia Song, Jingshu Chen, Yongxi Ma, Wei Ding, Xi Ma
This study was conducted to evaluate the effect of flavors on reproductive performance of sows and we also studied its effect on gut barrier function. Forty-eight Landrace × Yarkshire sows were randomly allotted and fed a basal diet added 0%, 0.05% or 0.10% flavor feed, respectively from parturition to day 28 of weaning. The results showed that supplementation of 0.05% or 0.10% flavors increased average daily feed intake (ADFI) of sows and average daily gain (ADG) of piglets, decreased the weight losses of sows, increased the survival ratio of weaning piglets (P < 0...
February 23, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28240158/biodiesel-production-from-citrillus-colocynthis-oil-using-based-catalytic-reaction-and-characterization-studies
#19
Imededdine A Nehdi, Hassen M Sbihi, Lahssen El Blidi, Umer Rashid, Saud I Al-Resayes
In this investigation, an enzyme catalyzed process was optimized to prepare methyl esters from crude Citrullus colocynthis oil (CCO). Novozym 435 was used to synthesize biodiesel by transesterification of CCO with methanol. Use of tert-butanol as a reaction medium eliminated negative effects caused by excess methanol and formation of glycerol byproduct. Several variables affecting the Citrullus colocynthis methyl ester (CCME) yield including lipase quantity, water content, methanol/oil mole ratio, reaction temperature, reaction medium composition, and reaction time were optimized...
February 23, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28240165/diosmetin-a-potential-p53-activator-performs-anticancer-effect-by-regulating-cell-cycling-and-cell-proliferation-in-hepg2-cells
#20
Bin Liu, Miaomiao Zhang, Shuna Liu, Ying Jie, Jingjing Zhang, Weiqiang Zheng, Kurihara Hiroshi, Runzhi Zhu
Diosmetin (Dios), a flavone aglycone, is a major active ingredient of many herbal medicines. Previous studies implicated that the potential antitumor activities of Dios are mediated through multiple cell signaling pathways, however more investigations are required to reveal the targets and mechanisms of Dios. In this study, our results demonstrated that cell cycle progression and proliferation of HepG2 cells were inhibited by Dios. Meanwhile, Dios-induced liver cancer cell apoptosis was related with the p53 activation...
February 22, 2017: Protein and Peptide Letters
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