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Xenotransplantation

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https://www.readbyqxmd.com/read/29322561/effect-of-an-oxygen-generating-scaffold-on-the-viability-and-insulin-secretion-function-of-porcine-neonatal-pancreatic-cell-clusters
#1
Eun Mi Lee, Ji-In Jung, Zahid Alam, Hee-Gyeong Yi, Heejin Kim, Jin Woo Choi, Sunghoon Hurh, Young June Kim, Jong Cheol Jeong, Jaeseok Yang, Kook-Hwan Oh, Hee Chan Kim, Byeong Chun Lee, Inho Choi, Dong-Woo Cho, Curie Ahn
BACKGROUND: Islet encapsulation techniques have shown limited success in maintaining islet survival and function because encapsulation decreases oxygen supply. In this study, an oxygen-generating scaffold was fabricated to prevent hypoxic cell damage and improve the viability and insulin secretion of islets. METHODS: We fabricated an oxygen-generating scaffold by mixing calcium peroxide (CaO2 ) with polydimethylsiloxane (PDMS). We evaluated the effects of the oxygen-generating PDMS + CaO2 scaffold on viability, caspase-3 and caspase-7 activity, oxygen consumption rate (OCR), glucose-stimulated insulin secretion (GSIS), hypoxic cell marker expression, and reactive oxygen species (ROS) levels in porcine neonatal pancreatic cell clusters (NPCCs)...
January 10, 2018: Xenotransplantation
https://www.readbyqxmd.com/read/29250828/cd4-cd25hi-foxp3-regulatory-t-cells-in-long-term-cardiac-xenotransplantation
#2
Avneesh K Singh, Joshua L Chan, Caleb N Seavey, Philip C Corcoran, Robert F Hoyt, Billeta G T Lewis, Marvin L Thomas, David L Ayares, Keith A Horvath, Muhammad M Mohiuddin
BACKGROUND: CD4+CD25Hi FoxP3+ T (Treg) cells are a small subset of CD4+ T cells that have been shown to exhibit immunoregulatory function. Although the absolute number of Treg cells in peripheral blood lymphocytes (PBL) is very small, they play an important role in suppressing immune reactivity. Several studies have demonstrated that the number of Treg cells, rather than their intrinsic suppressive capacity, may contribute to determining the long-term fate of transplanted grafts. In this study, we analyzed Treg cells in PBL of long-term baboon recipients who have received genetically modified cardiac xenografts from pig donors...
December 17, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29218794/examining-the-potential-for-porcine-derived-islet-cells-to-harbour-viral-pathogens
#3
Claire Crossan, Zoe O'Hara, Nizar Mourad, Pierre Gianello, Linda Scobie
With an onus on safety in the potential use of porcine islet cells as a treatment for diabetes, the use of animals lacking exogenous pathogens is clearly important and multilevel screening strategies have been presented on testing animals and the product. In this study, we wished to investigate whether islet cells indeed harboured the same viral pathogens of concern in the source animal. PMBC and islet cells from both adult and neonatal source animals were directly compared and tested for PCMV, PLHV, PCV2, PPV and HEV using both molecular and serological assays...
December 7, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29210476/delayed-revascularization-of-islets-after-transplantation-by-il-6-blockade-in-pig-to-non-human-primate-islet-xenotransplantation-model
#4
Byoung-Hoon Min, Jun-Seop Shin, Jong-Min Kim, Seong-Jun Kang, Hyun-Je Kim, Il-Hee Yoon, Su-Kyoung Park, Ji-Won Choi, Min-Suk Lee, Chung-Gyu Park
BACKGROUND: Pancreatic islet transplantation is currently proven as a promising treatment for type 1 diabetes patients with labile glycemic control and severe hypoglycemia unawareness. Upon islet transplantation, revascularization is essential for proper functioning of the transplanted islets. As IL-6 is important for endothelial cell survival and systemic inflammation related to xenograft, the effect of IL-6 receptor antagonist, tocilizumab, on revascularization of the transplanted islets was examined in pig to non-human primate islet xenotransplantation model...
December 6, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29193342/miscellaneous
#5
David K C Cooper, Peter Cowan, Jay A Fishman, Bernhard J Hering, Muhammad M Mohiuddin, Richard N Pierson, David H Sachs, Henk-Jan Schuurman, John U Dennis, Ralf R Tönjes
No abstract text is available yet for this article.
November 28, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29171695/meeting-report-the-first-international-conference-on-xenotransplantation-in-chengdu-china
#6
Xiang Li, Yi Wang, Shaoping Deng
No abstract text is available yet for this article.
November 24, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29171094/perv-inactivation-is-necessary-to-guarantee-absence-of-pig-to-patient-pervs-transmission-in-xenotransplantation
#7
Marc Güell, Dong Niu, Yinan Kan, Haydy George, Tao Wang, I-Hsiu Lee, Gang Wang, George Church, Luhan Yang
No abstract text is available yet for this article.
November 23, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29168238/xenotransplantation-literature-update-september-october-2017
#8
Eric M Walters, Christopher Burlak
No abstract text is available yet for this article.
November 22, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29135052/enhanced-effect-of-human-mesenchymal-stem-cells-expressing-human-tnf-%C3%AE-r-fc-and-ho-1-gene-on-porcine-islet-xenotransplantation-in-humanized-mice
#9
Han-Sin Lee, Sanghyun Song, Du Yeon Shin, Geun-Soo Kim, Jong-Hyun Lee, Chan Woo Cho, Kyo Won Lee, Hyojun Park, Curie Ahn, Jaeseok Yang, Heung-Mo Yang, Jae Berm Park, Sung-Joo Kim
BACKGROUND: Porcine islet xenotransplantation is considered an attractive alternative treatment for type 1 diabetes mellitus. However, it is largely limited because of initial rejection due to Instant Blood-Mediated Inflammatory Reaction (IBMIR), oxidative stress, and inflammatory responses. Recently, soluble tumor necrosis factor-ɑ receptor type I (sTNF-αR) and heme oxygenase (HO)-1 genes (HO-1/sTNF-αR) have been shown to improve the viability and functionality of porcine islets after transplantation...
November 14, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29131463/inactivation-of-porcine-endogenous-retrovirus-in-pigs-using-crispr-cas9-editorial-commentary
#10
Linda Scobie, Joachim Denner, Henk-Jan Schuurman
No abstract text is available yet for this article.
November 12, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29131417/culturing-with-modified-egm2-medium-enhances-porcine-neonatal-islet-like-cell-clusters-resistance-to-apoptosis-in-islet-xenotransplantation
#11
Xiaoqian Ma, Cejun Yang, Juan Zhang, Jia Wang, Wei Li, Chang Xu, Pengfei Rong, Bin Ye, Minghua Wu, Jianhui Jiang, Shounan Yi, Wei Wang
BACKGROUND: Neonatal pig islet-like cell clusters (NICC) are an attractive source of insulin-producing tissue for potential transplantation treatment of type 1 diabetic patients. However, a considerable loss of NICC after their transplantation due to apoptosis resulted from islet isolation and instant blood-mediated inflammatory reaction remains to be overcome. METHODS: EGM2 medium depleted with hydrocortisone and supplemented with 50 mmol/L isobutylmethylxanthine, 10 mmol/L nicotinamide, and 10 mmol/L glucose was used to culture NICC at day 1, the day after isolation and changed every other day...
November 12, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29131413/the-effect-of-epitope-based-ligation-of-icam-1-on-survival-and-retransplantation-of-pig-islets-in-nonhuman-primates
#12
Jae-Il Lee, Jiyeon Kim, Yun-Jung Choi, Hi-Jung Park, Hye-Jin Park, Hae Joo Wi, Sunok Yoon, Jun-Seop Shin, Jin Kyun Park, Kyeong Cheon Jung, Eun Bong Lee, Hee Jung Kang, Eung Soo Hwang, Sang-Joon Kim, Chung-Gyu Park, Seong Hoe Park
BACKGROUND: Pig islet xenotransplantation is a promising alternative to allogeneic transplantation. However, the wide immunologic barrier between pigs and primates limits the long-term survival of the graft. MD-3, a novel monoclonal antibody (mAb) that recognizes a particular epitope of human ICAM-1, can render T cells tolerant to a xenograft by arresting dendritic cell maturation. We report the long-term survival of adult wild-type pig islets and successful retransplantation in nonhuman primates using a protocol comprising induction with MD-3 mAb and maintenance with anti-CD154 mAb and sirolimus...
November 12, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29067747/effects-of-carbon-monoxide-on-early-dysfunction-and-microangiopathy-following-galt-ko-porcine-pulmonary-xenotransplantation-in-cynomolgus-monkeys
#13
Hisashi Sahara, Mitsuhiro Sekijima, Yuichi Ariyoshi, Akihiro Kawai, Kohei Miura, Shiori Waki, Louras Nathan, Yusuke Tomita, Takehiro Iwanaga, Kazuaki Nakano, Hitomi Matsunari, Hiroshi Date, Hiroshi Nagashima, Akira Shimizu, Kazuhiko Yamada
BACKGROUND: Despite progress in the current genetic manipulation of donor pigs, most non-human primates were lost within a day of receiving porcine lung transplants. We previously reported that carbon monoxide (CO) treatment improved pulmonary function in an allogeneic lung transplant (LTx) model using miniature swine. In this study, we evaluated whether the perioperative treatment with low-dose inhalation of CO has beneficial effects on porcine lung xenografts in cynomolgus monkeys (cynos)...
October 25, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29067741/synthetic-liver-function-is-detectable-in-transgenic-porcine-livers-perfused-with-human-blood
#14
Arielle Cimeno, Beth M French, Jessica M Powell, Carol Phelps, David Ayares, Natalie A O'Neill, Christopher T Laird, Richard N Pierson, Agnes M Azimzadeh, Rolf N Barth, John C LaMattina
In addition to immune barriers, molecular incompatibilities between species are predicted to limit pig liver survival in primate xenotransplantation models. Assessment and measurement of synthetic function of genetically modified porcine livers after ex vivo perfusion with human blood have not previously been described. Eight porcine livers from α1,3-galactosyl transferase knockout and human membrane cofactor (GalTKO.hCD46), six livers from GalTKO.hCD46 and N-glycolylneuraminic acid knockout (GalTKO.hCD46...
October 25, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29067714/low-anti-pig-antibody-levels-are-key-to-the-success-of-solid-organ-xenotransplantation-but-is-this-sufficient
#15
David K C Cooper, Mohamed B Ezzelarab, Hidetaka Hara
No abstract text is available yet for this article.
October 25, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29057561/pre-clinical-results-in-pig-to-non-human-primate-islet-xenotransplantation-using-anti-cd40-antibody-2c10r4-based-immunosuppression
#16
Jun-Seop Shin, Jong-Min Kim, Byoung-Hoon Min, Il Hee Yoon, Hyun Je Kim, Jung-Sik Kim, Yong-Hee Kim, Seong-Jun Kang, Jiyeon Kim, Hee-Jung Kang, Dong-Gyun Lim, Eung-Soo Hwang, Jongwon Ha, Sang-Joon Kim, Wan Beom Park, Chung-Gyu Park
BACKGROUND: Islet transplantation is an effective therapy for selected patients with type 1 diabetes with labile glycemic control and hypoglycemic unawareness, but donor organs are limited. Islet xenotransplantation using porcine islets will potentially solve this problem. Although successful proof of concept studies using clinically inapplicable anti-CD154 monoclonal antibody (mAb) in pig-to-non-human primate (NHP) islet xenotransplantation has been demonstrated by several groups worldwide, potentially clinically applicable anti-CD40 (2C10R4) mAb-based studies have not been reported...
October 22, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29057512/induction-of-porcine-specific-regulatory-t-cells-with-high-specificity-and-expression-of-il-10-and-tgf-%C3%AE-1-using-baboon-derived-tolerogenic-dendritic-cells
#17
Mingqian Li, Judith Eckl, Jan-Michael Abicht, Tanja Mayr, Bruno Reichart, Dolores J Schendel, Heike Pohla
BACKGROUND: Regulatory T cells (Treg) play an important role in maintenance of homeostasis in vivo. Treg application to alleviate allo-organ rejection is being studied extensively. However, natural Treg (nTreg) expansion in vitro is laborious and expensive. Antigen-specific Treg are more effective and require lower cell numbers than use of nTreg for immune control. The baboon, as a non-human primate experimental animal model, is widely used in xenotransplantation research. An effective method to generate baboon xeno-specific Treg would benefit research on immune tolerance in xenotransplantation using this model system...
October 22, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29057510/release-of-pig-leukocytes-and-reduced-human-nk-cell-recruitment-during-ex-vivo-perfusion-of-hla-e-human-cd46-double-transgenic-pig-limbs-with-human-blood
#18
Gisella Puga Yung, Anjan K Bongoni, Amandine Pradier, Natacha Madelon, Maria Papaserafeim, Riccardo Sfriso, David L Ayares, Eckhard Wolf, Nikolai Klymiuk, Andrea Bähr, Mihai A Constantinescu, Esther Voegelin, David Kiermeir, Hansjörg Jenni, Robert Rieben, Jörg D Seebach
BACKGROUND: In pig-to-human xenotransplantation, interactions between human natural killer (NK) cells and porcine endothelial cells (pEC) are characterized by recruitment and cytotoxicity. Protection from xenogeneic NK cytotoxicity can be achieved in vitro by the expression of the non-classical human leukocyte antigen-E (HLA-E) on pEC. Thus, the aim of this study was to analyze NK cell responses to vascularized xenografts using an ex vivo perfusion system of pig limbs with human blood...
October 22, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29057501/different-approaches-to-heart-valve-decellularization-a-comprehensive-overview-of-the-past-30%C3%A2-years
#19
REVIEW
Filippo Naso, Alessandro Gandaglia
Xenogeneic decellularized heart valve scaffolds have the potential to overcome the limitations of existing bioprosthetic heart valves that have limited duration due to calcification and tissue degeneration phenomena. This article presents a review of 30 years of decellularization approaches adopted in cardiovascular tissue engineering, with a focus on the use, either individually or in combination, of different detergents. The safety and efficacy of cell-removal procedures are specifically reported and discussed, as well as the structure and biomechanics of the treated extracellular matrix (ECM)...
October 22, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29057592/the-role-of-sialic-acids-in-the-immune-recognition-of-xenografts
#20
REVIEW
Beth M French, Selin Sendil, Richard N Pierson, Agnes M Azimzadeh
Presentation of sialic acid (Sia) varies among different tissues and organs within each species, and between species. This diversity has biologically important consequences regarding the recognition of cells by "xeno" antibodies (Neu5Gc vs Neu5Ac). Sia also plays a central role in inflammation by influencing binding of the asialoglycoprotein receptor 1 (ASGR-1), Siglec-1 (Sialoadhesin), and cellular interactions mediated by the selectin, integrin, and galectin receptor families. This review will focus on what is known about basic Sia structure and function in association with xenotransplantation, how changes in sialylation may occur in this context (through desialylation or changes in sialyltransferases), and how this fundamental pathway modulates adhesive and cell activation pathways that appear to be particularly crucial to homeostasis and inflammation for xenografts...
November 2017: Xenotransplantation
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