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Journal of Reproduction and Development

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https://www.readbyqxmd.com/read/28190810/visualization-of-the-spatial-arrangement-of-nuclear-organization-using-three-dimensional-fluorescence-in-situ-hybridization-in-early-mouse-embryos-a-new-easi-fish-chamber-glass-for-mammalian-embryos
#1
Masataka Nakaya, Hideyuki Tanabe, Shingo Takamatsu, Misaki Hosokawa, Tasuku Mitani
The fertilized oocyte begins cleavage, leading to zygotic gene activation (ZGA), which re-activates the resting genome to acquire totipotency. In this process, genomic function is regulated by the dynamic structural conversion in the nucleus. Indeed, a considerable number of genes that are essential for embryonic development are located near the pericentromeric regions, wherein the heterochromatin is formed. These genes are repressed transcriptionally in somatic cells. Three-dimensional fluorescence in situ hybridization (3D-FISH) enables the visualization of the intranuclear spatial arrangement, such as gene loci, chromosomal domains, and chromosome territories (CTs)...
February 11, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28163265/significant-heparin-effect-on-bovine-embryo-development-during-sexed-in-vitro-fertilization
#2
Li-You An, Sanjeev A Chaubal, Yanhong Liu, Yexiang Chen, Tshimangadzo L Nedambale, Jie Xu, Fei Xue, Juan F Moreno, Shenghong Tao, Giorgio Antonio Presicce, Fuliang DU
The aim of this study was to investigate the effect of different heparin concentrations in the course of sexed in vitro fertilization (IVF), on bovine embryonic development and development to term following embryo transfer (ET). With a total of 9156 oocytes for IVF, sorted as well as unsorted sperm from four bulls had different heparin requirements for achieving the highest rate of development in vitro. However, when optimal heparin concentrations were used (40 to 80 µg/ml), the performance of X-sorted sperm (0...
February 5, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28163263/decrease-in-body-surface-temperature-before-parturition-in-ewes
#3
Hisashi Nabenishi, Atushi Yamazaki
This study investigated the correlation between the body surface temperature (BST) and core body temperature of ewes and changes in BST during the prepartum stage in pregnant ewes. Four non-pregnant adult ewes were used in the first experiment. The BST of the upper neck, vaginal temperature (VT), and ambient temperature (AT) were measured every 10 min for seven days and analyzed for correlations. The mean (± SD) BST and VT of ewes during the study period were 35.4 ± 1.7°C and 39.1 ± 0.4°C, respectively, with a correlation of r = 0...
February 5, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28132978/positive-correlations-of-age-and-parity-with-plasma-anti-m%C3%A3-llerian-hormone-concentrations-in-japanese-black-cows
#4
Motoya Koizumi, Hiroya Kadokawa
Anti-Müllerian hormone (AMH) is secreted from the preantral and small antral follicles. It regulates follicle development and inhibits follicular atresia. This study examined how age, parity, and time after parturition affect plasma AMH concentrations in Japanese Black cows. We measured plasma AMH concentrations in primiparous, secundiparous, and multiparous (third parity or higher) cows at four time points: day 2 (day 0 = parturition), day 8, 2 days before first postpartum ovulation (pre-1stOv), and 12 days after first ovulation (post-1stOV)...
January 27, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28111381/establishment-of-dna-methylation-patterns-of-the-fibrillin1-fbn1-gene-in-porcine-embryos-and-tissues
#5
Yoshikazu Arai, Kazuhiro Umeyama, Kenta Takeuchi, Natsumi Okazaki, Naomi Hichiwa, Sayaka Yashima, Kazuaki Nakano, Hiroshi Nagashima, Jun Ohgane
DNA methylation in transcriptional regulatory regions is crucial for gene expression. The DNA methylation status of the edges of CpG islands, called CpG island shore, is involved in tissue/cell-type-specific gene expression. Haploinsufficiency diseases are caused by inheritance of one mutated null allele and are classified as autosomal dominant. However, in the same pedigree, phenotypic variances are observed despite the inheritance of the identical mutated null allele, including FBN1, which is responsible for development of the haploinsufficient Marfan disease...
January 22, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28090002/easy-detection-of-hormone-secretion-from-l%C3%AE-t2-cells-by-using-gaussia-luciferase
#6
Kazuhiro Satou, Yuta Mochimaru, Takashi Nakakura, Tomoyuki Kusada, Jun Negishi, Shiori Musha, Nanaka Yoshimura, Yukio Kato, Hideaki Tomura
Reproduction is regulated by gonadotropins secreted from gonadotrophs. The production and secretion of gonadotropins are mainly regulated by gonadotropin-releasing hormone (GnRH). Agonists or antagonists that influence GnRH action on gonadotrophs are important to regulate reproduction; however, these factors have not been fully characterized due to the lack of simple and easy-to-use techniques to detect gonadotropin secretion from gonadotropin-producing cells. In the present study, we found that Gaussia luciferase (Gluc), which was expressed in LβT2 cells, can be secreted like a luteinizing-hormone (LH) upon stimulation with GnRH...
January 15, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28070055/superovulatory-responses-in-cynomolgus-monkeys-macaca-fascicularis-depend-on-the-interaction-between-donor-status-and-superovulation-method-used
#7
Ji-Su Kim, Seung-Bin Yoon, Kang-Jin Jeong, Bo-Woong Sim, Seon-A Choi, Sang-Il Lee, Yeung Bae Jin, Bong-Seok Song, Sang-Rae Lee, Sun-Uk Kim, Kyu-Tae Chang
The current study was performed to investigate the effect of oocyte donor status, including age and body weight, on metaphase II (MII) oocyte recovery using two superovulation methods in cynomolgus monkeys. The use of Method A [recombinant gonadotrophin (75 IU/kg, 3×, 3-day intervals) and human chorionic gonadotropin (hCG)] led to great increases in ovary size and the mean number of MII oocytes retrieved in age- and body-weight-dependent manner; in contrast, both the parameters were similar in Method B [recombinant gonadotrophin (60 IU, twice daily, 6 days), recombinant gonadotropin and recombinant human luteinizing hormone (rhLH) (60 IU, twice daily, 3 days), and hCG]...
January 9, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28163264/effects-of-exosome-like-vesicles-on-cumulus-expansion-in-pigs-in-vitro
#8
Yuta Matsuno, Asuka Onuma, Yoshie A Fujioka, Kazuma Yasuhara, Wataru Fujii, Kunihiko Naito, Koji Sugiura
Cell-secreted vesicles, such as exosomes, have recently been recognized as mediators of cell communication. A recent study in cattle showed the involvement of exosome-like vesicles in the control of cumulus expansion, a prerequisite process for normal ovulation; however, whether this is the case in other mammalian species is not known. Therefore, this study aimed to examine the presence of exosome-like vesicles in ovarian follicles and their effects on cumulus expansion in vitro in pigs. The presence of exosome-like vesicles in porcine follicular fluid (pFF) was confirmed by transmission electron microscopic observation, the detection of marker proteins, and RNA profiles specific to exosomes...
5, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28049872/involvement-of-calcium-channels-and-intracellular-calcium-in-bull-sperm-thermotaxis
#9
Md Anisuzzaman Mondal, Yuji Takagi, Shoji A Baba, Koh-Ichi Hamano
Thermotaxis that sperm migrate to higher temperature area has been confirmed in rabbit and human. In this study, we examined the migration ability of bull sperm in a temperature gradient to confirm thermotaxis and elucidate the involvement of calcium in such thermotaxis, as well as the relation between sperm capacitation and bull fertility. Thermotaxis was evaluated in a temperature gradient of 34-42ºC using a cross-type column 22-mm long, 40-mm wide, and 100-μm deep. Significantly more sperm migrated to the high-temperature area of 39ºC in a 2ºC temperature gradient, and to 40ºC in a 1ºC temperature gradient...
December 31, 2016: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/27980237/prediction-of-blastocyst-development-and-implantation-potential-in-utero-based-on-the-third-cleavage-and-compaction-times-in-mouse-pre-implantation-embryos
#10
Jihyun Kim, Seok Hyun Kim, Jin Hyun Jun
Cytokinesis and cell division during pre-implantation embryonic development occur as an orchestrated spatiotemporal program. Cleavage, compaction, and blastulation in pre-implantation embryos are essential for successful implantation and pregnancy. Their alteration is associated with chromosomal imbalance and loss of developmental competence. In this study, we evaluated the time of cleavage and compaction as predictors for in vitro pre- and peri-implantation development and in utero implantation potential by time-lapse monitoring...
December 16, 2016: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/27980236/expression-of-uterine-lipocalin-2-and-its-receptor-during-early-to-mid-pregnancy-period-in-mares
#11
Shingo Haneda, Kentaro Nagaoka, Yasuo Nambo, Masato Kikuchi, Yasuko Nakano, Junyou Li, Motozumi Matsui, Yo-Ichi Miyake, Kazuhiko Imakawa
From previous cDNA subtraction studies analyzing gene expression in equine endometrium, high lipocalin 2 (LCN2) mRNA expression was found in the gravid endometrium. In the uterus, LCN2 may transport hydrophobic molecules and siderophores with iron, or may form a complex with another protein, however, the expression of uterine LCN2 beyond day 20 of equine pregnancy and its receptor has not been characterized. To study the expression and potential roles of uterine LCN2 from pre-implantation to mid-gestation period, stage-specific endometrial samples were obtained from day 13 (day 0 = ovulation) cyclic and days 13, 19, 25, and 60 to 131 pregnant mares...
December 16, 2016: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/27941302/effects-of-downregulating-tead4-transcripts-by-rna-interference-on-early-development-of-bovine-embryos
#12
Nobuyuki Sakurai, Kazuki Takahashi, Natsuko Emura, Tsutomu Hashizume, Ken Sawai
Transcription factor TEA domain family transcription factor 4 (Tead4) is one of the key factors involved in the differentiation of the trophectoderm (TE) in murine embryos. However, knowledge on the roles of TEAD4 in preimplantation development during bovine embryos is currently limited. This study examined the transcript and protein expression patterns of TEAD4 and attempted to elucidate the functions of TEAD4 during bovine preimplantation development using RNA interference. TEAD4 mRNA was found to be upregulated between the 16-cell and morula stages, and nuclear localization of the TEAD4 protein was detected at the morula stage, as well as in subsequent developmental stages...
December 11, 2016: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/27904014/male-infertility-related-molecules-involved-in-sperm-oocyte-fusion
#13
Lisha Mou, Ni Xie
Male infertility has become a very serious problem in the human reproduction system, but the molecular mechanism of infertility remains largely unknown. Fertilization is the phenomenon in which a sperm and oocyte find each other, interact, and fuse. Sperm-oocyte fusion-related factors on the sperm side play crucial roles in male infertility. For example, IZUMO1 is well-known as a sperm protein essential for fusion of a sperm and oocyte, but its dysfunction or mutation can result in male infertility. Recent studies showed a novel sperm protein named Bactericidal/permeability-increasing protein (BPI), which takes part in the sperm-oocyte fusion process...
December 1, 2016: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/27890874/development-of-a-lipopolysaccharide-lps-supplemented-adjuvant-and-its-effects-on-cell-mediated-and-humoral-immune-responses-in-male-rats-immunized-against-sperm
#14
Junko Noguchi, Shinya Watanabe, Thanh Q Dang Nguyen, Kazuhiro Kikuchi, Hiroyuki Kaneko
Supplementation with lipopolysaccharide (LPS) from non-pathogenic Escherichia coli was found to enhance the adjuvant effects of a veterinary vaccine adjuvant (ISA 71VG(®)). Sperm immunization using 71VG as an adjuvant in the immature period induced infertility in 25% of male rats, whereas this increased to 62.5% after immunization with 71VG + LPS or Freund's complete adjuvant (FCA). Mean testicular weight of non-sterile males in the 71VG + LPS group was significantly lower than that in the 71VG or FCA group...
November 28, 2016: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/27890873/overabundance-of-sika-deer-and-immunocontraception
#15
Junko Noguchi
The impact of deer overabundance is a worldwide problem. Along with habitat expansion and population increase, damage by sika deer to the forest ecosystem and agriculture has become a serious issue in Japan. Deer also transmit a number of diseases and parasites to humans and livestock. The overabundance of deer is a result of their strong fecundity, and therefore the present situation should, in theory, be tackled by experts in reproductive biology.
November 28, 2016: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/27904015/deletion-of-conserved-sequences-in-ig-dmr-at-dlk1-gtl2-locus-suggests-their-involvement-in-expression-of-paternally-expressed-genes-in-mice
#16
Takeshi Saito, Satoshi Hara, Moe Tamano, Hiroshi Asahara, Shuji Takada
Expression regulation of the Dlk1-Dio3 imprinted domain by the intergenic differentially methylated region (IG-DMR) is essential for normal embryonic development in mammals. In this study, we investigated conserved IG-DMR genomic sequences in eutherians to elucidate their role in genomic imprinting of the Dlk1-Dio3 domain. Using a comparative genomics approach, we identified three highly conserved sequences in IG-DMR. To elucidate the functions of these sequences in vivo, we generated mutant mice lacking each of the identified highly conserved sequences using the CRISPR/Cas9 system...
February 16, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/27725347/lack-of-calcium-oscillation-causes-failure-of-oocyte-activation-after-intracytoplasmic-sperm-injection-in-pigs
#17
Michiko Nakai, Junya Ito, Shun-Ichi Suzuki, Dai-Ichiro Fuchimoto, Shoichiro Sembon, Misae Suzuki, Junko Noguchi, Hiroyuki Kaneko, Akira Onishi, Naomi Kashiwazaki, Kazuhiro Kikuchi
In pigs, the efficiency of embryo production after intracytoplasmic sperm injection (ICSI) is still low because of frequent failure of normal fertilization, which involves formation of two polar bodies and two pronuclei. To clarify the reasons for this, we hypothesized that ICSI does not properly trigger sperm-induced fertilization events, especially intracellular Ca(2+) signaling, also known as Ca(2+) oscillation. We also suspected that the use of in vitro-matured oocytes might negatively affect fertilization events and embryonic development of sperm-injected oocytes...
December 20, 2016: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/27725346/efficient-pig-icsi-using-percoll-selected-spermatozoa-evidence-for-the-essential-role-of-phospholipase-c-%C3%AE-in-icsi-success
#18
Michiko Nakai, Shun-Ichi Suzuki, Junya Ito, Dai-Ichiro Fuchimoto, Shoichiro Sembon, Junko Noguchi, Akira Onishi, Naomi Kashiwazaki, Kazuhiro Kikuchi
In pigs, the damaged sperm membrane leads to leakage of phospholipase C-ζ (PLCζ), which has been identified as a sperm factor, and a reduction of oocyte-activating ability. In this study, we investigated whether sperm selected by Percoll gradient centrifugation (Percoll) have sufficient PLCζ, and whether the efficiency of fertilization and blastocyst formation after intracytoplasmic sperm injection (ICSI) using Percoll-selected sperm can be improved. Percoll-selected sperm (Percoll group) or sperm without Percoll selection (Control group) were used...
December 20, 2016: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/27665783/meiotic-cohesin-subunits-rad21l-and-rec8-are-positioned-at-distinct-regions-between-lateral-elements-and-transverse-filaments-in-the-synaptonemal-complex-of-mouse-spermatocytes
#19
Mei Rong, Atsushi Matsuda, Yasushi Hiraoka, Jibak Lee
Cohesins containing a meiosis-specific α-kleisin subunit, RAD21L or REC8, play roles in diverse aspects of meiotic chromosome dynamics including formation of axial elements (AEs), assembly of the synaptonemal complex (SC), recombination of homologous chromosomes (homologs), and cohesion of sister chromatids. However, the exact functions of individual α-kleisins remain to be elucidated. Here, we examined the localization of RAD21L and REC8 within the SC by super-resolution microscopy, 3D-SIM. We found that both RAD21L and REC8 were localized at the connection sites between lateral elements (LEs) and transverse filaments (TFs) of pachynema with RAD21L locating interior to REC8 sites...
December 20, 2016: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/27647534/adenylation-by-testis-specific-cytoplasmic-poly-a-polymerase-papolb-tpap-is-essential-for-spermatogenesis
#20
Shin-Ichi Kashiwabara, Satsuki Tsuruta, Keitaro Okada, Yutaro Yamaoka, Tadashi Baba
The testis-specific cytoplasmic poly(A) polymerase PAPOLB/TPAP is essential for spermatogenesis. Although this enzyme is responsible for poly(A) tail extension of a subset of mRNAs in round spermatids, the stability and translational efficiency of these mRNAs are unaffected by the absence of PAPOLB. To clarify the functional importance of this enzyme's adenylation activity, we produced PAPOLB-null mice expressing a polyadenylation-defective PAPOLB mutant (PAPOLB(D114A)), in which the catalytic Asp at residue 114 was mutated to Ala...
December 20, 2016: Journal of Reproduction and Development
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