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Journal of Reproduction and Development

Kohta Kikuchi, Keisuke Kozai, Takuo Hojo, Miki Sakatani, Kiyoshi Okuda, Hanako Bai, Manabu Kawahara, Masashi Takahashi
We investigated the electrical impedance of the reproductive tracts (vagina and uterine endometrial tissues) and the expression of mucus-related genes to identify the stage of the estrous cycle in mares. We first examined vaginal impedance in native Hokkaido mares during their estrous cycle and found no significant differences. However, impedance levels tended to decrease towards ovulation. Furthermore, we investigated the estrous cycle by measuring the electrical impedance of the uterine endometrial tissues obtained from carcasses of mares...
January 7, 2018: Journal of Reproduction and Development
Hisashi Funakura, Ayumi Shiki, Yuji Tsubakishita, Shogo Mido, Hiromu Katamoto, Go Kitahara, Takeshi Osawa
This study aimed to clarify the feasibility of a novel timed artificial insemination (TAI) protocol using ultrasonography, and to determine the associations between the ovarian component and fertility. In Experiment 1,272 Japanese Black cows with a corpus luteum (CL) ≥ 18 mm in diameter were divided randomly into either the TRT group (134 cows that were administered gonadotropin-releasing hormone [GnRH] 56 h [day 2] after prostaglandin F2α [PGF] administration [day 0], followed by TAI 16-20 h later) or the CN-1 group (138 cows that were administered PGF followed by AI after estrus detection)...
January 5, 2018: Journal of Reproduction and Development
Nan Zhang, Wei Mao, Ying Zhang, Na Huang, Bo Liu, Long Gao, Shuangyi Zhang, Jinshan Cao
Oviductal glycoprotein 1 (OVGP1), an oviductin, is involved in the maintenance of sperm viability and motility and contributes to sperm capacitation in the oviduct. In this study, the regulatory effects exerted by prostaglandin E2 (PGE2) and F2α (PGF2α) on OVGP1 expression via their corresponding receptors in bovine oviductal epithelial cells (BOECs) were investigated. BOECs were cultured in vitro, and their expression of receptors of PGE2 (PTGER1, PTGER2, PTGER3, and PTGER4) and PGF2α (PTGFR) was measured using RT-qPCR...
December 23, 2017: Journal of Reproduction and Development
Jinsha Liu, Keiji Mochida, Ayumi Hasegawa, Kimiko Inoue, Atsuo Ogura
Although it is known that the susceptibility of mouse spermatozoa to freezing-thawing varies greatly with genetic background, the underlying mechanisms remain to be elucidated. In this study, to map genetic regions responsible for the susceptibility of spermatozoa to freezing-thawing, we performed in vitro fertilization using spermatozoa from recombinant inbred mice derived from the C57BL/6J and DBA/2J strains, whose spermatozoa showed distinct fertilization abilities after freezing. Genome-wide interval mapping identified two suggestive quantitative trait loci (QTL) associated with fertilization on chromosomes 1 and 11...
December 21, 2017: Journal of Reproduction and Development
Ren Watanabe, Naoko Kimura
Around the time of oocyte meiotic arrest, germ cell nest breakdown occurs, and primordial follicle (PF) formation is initiated at the perinatal stage. Recently, autophagy was implicated in this process. Autophagy is induced by nutrient starvation. This study was conducted to understand how starvation affects PF formation and autophagy induction during neonatal life. Suckling of neonatal female mice was blocked immediately after birth for 12-36 h to induce starvation. The numbers of PFs at each stage were subsequently counted from serial sections of ovaries...
December 21, 2017: Journal of Reproduction and Development
Ying Lin, Liu-Cai Sui, Rong-Hua Wu, Ru-Jun Ma, Hai-Yan Fu, Juan-Juan Xu, Xu-Hua Qiu, Li Chen
Brusatol, a quassinoid isolated from the fruit of Bruceajavanica, has recently been shown to inhibit nuclear factor erythroid 2-related factor 2 (Nrf2) via Keap1-dependent ubiquitination and proteasomal degradation or protein synthesis. Nrf2 is a transcription factor that regulates the cellular defense response. Most studies have focused on the effects of Nrf2in tumor development. Here, the critical roles of Nrf2 in mouse early embryonic development were investigated. We found that brusatol treatment at the zygotic stage prevented the early embryo development...
December 16, 2017: Journal of Reproduction and Development
Tungmahasuk Doungrut, Numfa Fungbun, Titaree Laoharatchatathanin, Ryota Terashima, Shiro Kurusu, Mitsumori Kawaminami
Although the expression of gonadotropin-releasing hormone (GnRH) in the ovaries is well established, its physiological role remains unknown. The aim of this study was to determine whether ovarian GnRH mediates the actions of human chorionic gonadotropin (hCG) in the granulosa cells of immature female rats. Follicular growth was induced by administration of pregnant mare serum gonadotropin (PMSG, 15 IU/0.15 ml) on day 25 after birth, and hCG (20 IU/0.2 ml) was administered on day 27 to increase the plasma levels of progesterone...
December 15, 2017: Journal of Reproduction and Development
Maria Grazia Palmerini, Manuel Belli, Stefania Annarita Nottola, Selenia Miglietta, Serena Bianchi, Sara Bernardi, Sevastiani Antonouli, Sandra Cecconi, Giuseppe Familiari, Guido Macchiarelli
Mancozeb, an ethylene bis-dithiocarbamate, is widely used as a fungicide and exerts reproductive toxicity in vivo and in vitro in mouse oocytes by altering spindle morphology and impairing the ability to fertilize. Mancozeb also induces a premalignant status in mouse granulosa cells (GCs) cultured in vitro, as indicated by decreased p53 expression and tenuous oxidative stress. However, the presence and extent of ultrastructural alterations induced by mancozeb on GCs in vitro have not yet been reported. Using an in vitro model of reproductive toxicity, comprising parietal GCs from mouse antral follicles cultured with increasing concentrations of mancozeb (0...
December 11, 2017: Journal of Reproduction and Development
Akane Sato, Borjigin Sarentonglaga, Kazuko Ogata, Mio Yamaguchi, Asuka Hara, Khurchabiling Atchalalt, Naoko Sugane, Rika Fukumori, Yoshikazu Nagao
The maturation rate of canine oocytes during in vitro maturation (IVM) needs to be improved. The present study was designed to evaluate the effects of insulin-like growth factor-1 (IGF-1) on the IVM of canine oocytes. Ovaries were obtained by ovariohysterectomy and were sliced to release cumulus-oocyte complexes (COCs). In Experiment 1, the effects of different concentrations of IGF-1 on the nuclear maturation of oocytes was investigated. The COCs were cultured in a modified medium (mTCM199) with IGF-1 (0, 0...
December 7, 2017: Journal of Reproduction and Development
Chika Higuchi, Natsumi Shimizu, Seung-Wook Shin, Kohtaro Morita, Kouhei Nagai, Masayuki Anzai, Hiromi Kato, Tasuku Mitani, Kazuo Yamagata, Yoshihiko Hosoi, Kei Miyamoto, Kazuya Matsumoto
Maternal RNA/protein degradation and zygotic genome activation (ZGA), occurring during maternal-to-zygotic transition (MZT), are the first essential events for the development of pre-implantation embryos. Previously, we have shown the importance of the ubiquitin-proteasome system (UPS) for initiation of minor ZGA at the 1-cell stage of mouse embryos. However, little is known about the mechanism of involvement of the UPS-degraded maternal proteins in ZGA. In this study, we investigated the effect of inhibiting maternal protein degradation by the reversible proteasome inhibitor, MG132, on post-implantation development and ZGA regulation during early cleavage stages...
December 7, 2017: Journal of Reproduction and Development
Kohta Kikuchi, Keisuke Sasaki, Hiroki Akizawa, Hayato Tsukahara, Hanako Bai, Masashi Takahashi, Yasuo Nambo, Hiroshi Hata, Manabu Kawahara
Insulin-like growth factor 2 (IGF2) is responsible for a broad range of physiological processes during fetal development and adulthood, but genomic analyses of IGF2 containing the 5'- and 3'-untranslated regions (UTRs) in equines have been limited. In this study, we characterized the IGF2 mRNA containing the UTRs, and determined its expression pattern in the fetal tissues of horses. The complete equine IGF2 mRNA sequence harboring another exon approximately 2.8 kb upstream from the canonical transcription start site was identified as a new transcript variant...
November 17, 2017: Journal of Reproduction and Development
Yuko Toishi, Nobuo Tsunoda, Shun-Ichi Nagata, Rikio Kirisawa, Kentaro Nagaoka, Gen Watanabe, Yojiro Yanagawa, Seiji Katagiri, Kazuyoshi Taya
Testosterone (T) concentration is a useful indicator of reproductive function in male animals. However, T concentration is not usually measured in veterinary clinics, partly due to the unavailability of reliable and rapid assays for animal samples. In this study, a rapid chemiluminescent enzyme immunoassay system (CLEIA system) that was developed for the measurement of T concentration in humans use was validated for stallion blood samples. First, serum T concentrations were measured using the CLEIA system and compared with those measured by a fluoroimmunoassay that has been validated for use in stallions...
November 11, 2017: Journal of Reproduction and Development
Hiroaki Okamura, Takashi Yamamura, Yoshihiro Wakabayashi
A population of neurons in the arcuate nucleus (ARC) coexpresses kisspeptin, neurokinin B (NKB), and dynorphin, and therefore they are referred to as KNDy neurons. It has been suggested that KNDy neurons participate in several brain functions, including the control of reproduction. The present study aimed to advance our understanding of the anatomy of the KNDy neural system. We first produced an antiserum against goat kisspeptin. After confirming its specificity, the antiserum was used to histochemically detect kisspeptin-positive signals...
November 7, 2017: Journal of Reproduction and Development
Shin-Ichi Kashiwabara, Satsuki Tsuruta, Yutaro Yamaoka, Kanako Oyama, Chieko Iwazaki, Tadashi Baba
Mutant mice lacking a testis-specific cytoplasmic poly(A) polymerase, PAPOLB/TPAP, exhibit spermiogenesis arrest and male infertility. However, the mechanism by which PAPOLB regulates spermiogenesis remains unclear. In this study, we examined the relationships between PAPOLB and other spermiogenesis regulators present in the chromatoid body (CB). The loss of PAPOLB had no impact either on the abundance of CB components such as PIWIL1, TDRD6, YBX2, and piRNAs, or on retrotransposon expression. In addition, localization of CB proteins and CB architecture were both normal in PAPOLB-null mice...
November 3, 2017: Journal of Reproduction and Development
Iulian Ibănescu, Claus Leiding, Heinrich Bollwein
This study aimed to identify motile sperm subpopulations in extended boar semen and to observe the presumptive seasonal variation in their distribution. Data from 4837 boar ejaculates collected over a two-year period were analyzed in terms of kinematic parameters by Computer Assisted Sperm Analysis (CASA). Individual sperm data were used to determine subgroups of motile sperm within the ejaculates using cluster analysis. Four motile sperm subpopulations (SP) were identified, with distinct movement patterns: SP1 sperm with high velocity and high linearity; SP2 sperm with high velocity but low linearity; SP3 sperm with low velocity but high linearity; and SP4 sperm with low velocity and low linearity...
October 28, 2017: Journal of Reproduction and Development
Alexander A Tokmakov, Ken-Ichi Sato, Vasily E Stefanov
Spawned unfertilized eggs have been found to die by apoptosis in several species with external fertilization. However, there is no necessity for the externally laid eggs to degrade via this process, as apoptosis evolved as a mechanism to reduce the damaging effects of individual cell death on the whole organism. The recent observation of egg degradation in the genital tracts of some oviparous species provides a clue as to the physiological relevance of egg apoptosis in these animals. We hypothesize that egg apoptosis accompanies ovulation in species with external fertilization as a normal process to eliminate mature eggs retained in the genital tract after ovulation...
October 27, 2017: Journal of Reproduction and Development
Ihsan Ali, Hai Xing Liu, Li Zhong-Shu, Ma Dong-Xue, Lijie Xu, Syed Zahid Ali Shah, Obaid Ullah, Fang Nan-Zhu
Endoplasmic reticulum (ER) stress, a dysfunction in protein-folding capacity, is involved in many pathological and physiological responses, including embryonic development. This study aims to determine the developmental competence, apoptosis, and stress-induced gene expression in mouse preimplantation embryos grown in an in vitro culture medium supplemented with different concentrations of the ER stress inducer tunicamycin (TM) and the antioxidant glutathione (GSH). Treatment of zygotes with 0.5 µg/ml TM significantly decreased (P < 0...
October 27, 2017: Journal of Reproduction and Development
Toshiaki Sumiyoshi, Natumi Endo, Tomomi Tanaka, Hideo Kamomae
Relaxation of the intravaginal part of the uterus is obvious around 6 to 18 h before ovulation, and this is considered the optimal time for artificial insemination (AI), as demonstrated in recent studies. Estrous signs have been suggested as useful criteria for determining the optimal time for AI. Therefore, this study evaluated the usefulness of estrous signs, particularly the relaxation of the intravaginal part of the uterus, as criteria for determining the optimal time for AI. A Total of 100 lactating Holstein-Friesian cows kept in tie-stall barns were investigated...
October 27, 2017: Journal of Reproduction and Development
Hiromi Kusaka, Hiroshi Miura, Motohiro Kikuchi, Minoru Sakaguchi
After parturition, the ovary ipsilateral to the side of previous pregnancy exhibits lower functional activity than that exhibited by the contralateral ovary. The local inhibitory effects of the corpus luteum of the previous pregnancy, and/or the presence of a previous gravid uterine horn, may induce the ipsilateral suppression of folliculogenesis. We examined the influence of the side of previous pregnancy on ovulation and folliculogenesis, until completion of the third postpartum ovulation. The ovaries of 30 Holstein cows were scanned by ultrasonography, through the three postpartum ovulation sequences...
October 26, 2017: Journal of Reproduction and Development
Toshimichi Ishii, Kensuke Tomita, Hideo Sakakibara, Satoshi Ohkura
This study was aimed at evaluating the effects of multi-layered cumulus cells (MCCs) during vitrification and in vitro fertilization (IVF) of mature bovine oocytes and embryogenesis after IVF. The rates of cleavage and blastocyst formation were higher in vitrified and fertilized oocytes with MCCs than in denuded oocytes (P < 0.05), but were comparable to the rates in fresh oocytes with MCCs or without (denuded). When the MCC-enclosed oocytes were denuded before IVF, blastocyst formation rate reduced compared with that in vitrified oocytes with MCCs (P < 0...
October 20, 2017: Journal of Reproduction and Development
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