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Journal of Reproduction and Development

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https://www.readbyqxmd.com/read/30626786/pre-ovulatory-follicular-temperature-in-bi-ovular-cows
#1
Fernando López-Gatius, Ronald H F Hunter
In a previous study on monovular cows, follicles revealed a mean antral (follicular fluid) temperature 1.54°C cooler than rectal temperatures in ovulating cows, whereas no such temperature differences were detected in non-ovulating cows. The present study adds to our previous work, this time considering 24 bi-ovular cows (one follicle per ovary). In order to increase the number of pre-ovulatory follicles failing to ovulate, this study was performed under heat-stress conditions. Follicular temperatures of the ovulating follicles (n = 31) were 0...
January 10, 2019: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/30613052/an-azoospermic-factor-gene-ddx3y-and-its-paralog-ddx3x-are-dispensable-in-germ-cells-for-male-fertility
#2
Takafumi Matsumura, Tsutomu Endo, Ayako Isotani, Masaki Ogawa, Masahito Ikawa
About 10% of male infertile patients show abnormalities in spermatogenesis. The microdeletion of azoospermia factor a (AZFa) region of the Y chromosome is thought to be a cause of spermatogenic failure. However, candidate gene responsible for the spermatogenic failure in AZFa deleted patients has not been elucidated yet. Using mice, we explored the function of Ddx3y, a strong candidate gene in the AZFa region, and Ddx3x, a Ddx3y paralog on the X chromosome, in spermatogenesis. We first generated Ddx3y KO male mice using CRISPR/Cas9 and found that the Ddx3y KO male mice show normal spermatogenesis, produce morphologically normal spermatozoa, and sire healthy offspring...
January 7, 2019: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/30606959/behavior-of-acrbp-deficient-mouse-sperm-in-the-female-reproductive-tract
#3
Kiyoshi Nagashima, Tomoyuki Usui, Tadashi Baba
Gene-knockout mice lacking ACRBP, a proacrosin-binding protein localized in the acrosome of sperm, have been shown to exhibit male subfertility, owing to abnormal formation of the acrosome. In this study, to elucidate the mechanism contributing to the subfertility phenotype, we examined the behavior of ACRBP-deficient mouse sperm in the female reproductive tract. When sperm that had migrated into the uterus and oviduct after mating were counted, the number of ACRBP-deficient sperm was noticeably smaller in the oviduct of mice post mating...
December 29, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/30606958/epigenetic-impairments-in-development-of-parthenogenetic-preimplantation-mouse-embryos
#4
Ngan Thi Kim Ho, Thuy Van Thi Nguyen, Thuan Van Nguyen, Hong-Thuy Bui
Parthenogenesis is an activation process of oocytes that occur without the participation of sperm. Evidence suggests that normal development of embryos requires proper expression of several imprinted genes inherited from both the paternal and maternal genomes. Compared to gene expression, histone modifications and chromatin remodeling are not well-documented. In this research, by using immunofluorescence staining for several developmental-associated histone modifications, we investigated whether epigenetic impairments in parthenogenetic embryos act as constraints for proper development...
December 29, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/30606957/resveratrol-supplementation-during-in-vitro-maturation-improves-embryo-development-of-prepubertal-goat-oocytes-selected-by-brilliant-cresyl-blue-staining
#5
Anna-Rita Piras, Irene Menéndez-Blanco, Sandra Soto-Heras, Maria-Gracia Catalá, Dolors Izquierdo, Luisa Bogliolo, Maria-Teresa Paramio
This study aimed to investigate the effect of resveratrol supplementation in maturation medium on the developmental ability and bioenergetic\oxidative status of prepubertal goat oocytes selected by brilliant cresyl blue (BCB). Oocytes collected from slaughterhouse-derived ovaries were selected by 13 µM BCB staining and classified as grown BCB+ and growing BCB- oocytes. All oocytes were matured in vitro in our conventional maturation medium and supplemented with 1 µM (BCB+R and BCB-R) and without (Control groups: BCB+C and BCB-C) resveratrol...
December 29, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/30587665/improved-early-development-of-porcine-cloned-embryos-by-treatment-with-quisinostat-a-potent-histone-deacetylase-inhibitor
#6
Anukul Taweechaipaisankul, Jun-Xue Jin, Sanghoon Lee, Geon A Kim, Yoon Ho Suh, Min Seok Ahn, Se Jun Park, Byeong You Lee, Byeong Chun Lee
Recently, the modification of the epigenetic status of somatic cell nuclear transfer (SCNT) embryos by treatment with histone deacetylase inhibitors (HDACis) has made it possible to alter epigenetic traits and improve the developmental competence of these embryos. In the current study, we examined the effects of an HDACi, quisinostat (JNJ), on the in vitro development of porcine cloned embryos and their epigenetic nuclear reprogramming status. SCNT embryos were cultured under various conditions, and we found that treatment with 100 nM JNJ for 24 h post activation could improve blastocyst formation rates compared to the control (P < 0...
December 27, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/30518723/off-and-on-target-effects-of-genome-editing-in-mouse-embryos
#7
Shinya Ayabe, Kenichi Nakashima, Atsushi Yoshiki
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-based genome editing technology has enabled manipulation of the embryonic genome. Unbiased whole genome sequencing comparing parents to progeny has revealed that the rate of Cas9-induced mutagenesis in mouse embryos is indistinguishable from the background rate of de novo mutation. However, establishing the best practice to confirm on-target alleles of interest remains a challenge. We believe that improvement in editing strategies and screening methods for founder mice will contribute to the generation of quality-controlled animals, thereby ensuring reproducibility of results in animal studies and advancing the 3Rs (replacement, reduction, and refinement)...
December 6, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/30487374/effect-of-hypoxia-on-progesterone-production-by-cultured-bovine-early-and-mid-luteal-cells
#8
Hiroki Hasegawa, Ryo Nishimura, Masamichi Yamashita, Takeshi Yamaguchi, Mitsugu Hishinuma, Kiyoshi Okuda
A major role of the corpus luteum (CL) is to produce progesterone (P4). The CL has immature vasculature shortly after ovulation, suggesting it exists under hypoxic conditions. To elucidate the mechanism involved in regulation of luteal cell function during CL development, we compared the effect of hypoxia on P4 production by cultured bovine early and mid luteal cells. Luteal cells obtained from early and mid CL were incubated under different O2 concentrations (20% and 3%) with or without hCG (1 U/ml) for 6 h and 24 h...
November 28, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/30464155/destabilization-of-spindle-assembly-checkpoint-causes-aneuploidy-during-meiosis-ii-in-murine-post-ovulatory-aged-oocytes
#9
Gaku Shimoi, Masaru Tomita, Marino Kataoka, Yuichi Kameyama
Mammalian oocyte quality degrades over time after ovulation in vitro, which can cause fatal defects such as chromosomal aneuploidy. As various oocyte manipulations employed in assisted reproductive technology are time consuming, post-ovulatory aging is a serious problem to overcome in reproductive medicine or ova research. In this study, we investigated the effects of postovulatory aging on the incidence of chromosome aneuploidy during meiosis II, with a focus on the expression of functional proteins from the spindle assembly checkpoint (SAC)...
November 22, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/30449821/evidence-for-the-involvement-of-fxr-signaling-in-ovarian-granulosa-cell-function
#10
Kentaro Takae, Mizuho Nakata, Takafumi Watanabe, Hiroshi Sasada, Hiroshi Fujii, Ikuo Tomioka
FXR (farnesoid X receptor) is mainly present in enterohepatic tissues and regulates cholesterol, lipid, and glucose homeostasis in coordination with target genes such as SHP and FABP6. Although FXR has been revealed to be expressed in reproductive tissues, FXR function and expression levels in the ovary remain unknown. In this study, we investigated FXR expression in mouse ovaries and its target genes in ovarian granulosa cells. In situ hybridization and immunohistochemical staining showed that FXR was mainly distributed in secondary and tertiary follicles...
November 16, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/30429414/pterostilbene-alleviates-hydrogen-peroxide-induced-oxidative-stress-via-nuclear-factor-erythroid-2-like-2-pathway-in-mouse-preimplantation-embryos
#11
Obaid Ullah, Zhongshu Li, Ihsan Ali, Lijie Xu, Haixing Liu, Syed Zahid Ali Shah, Nanzhu Fang
Pterostilbene (PTS) in blueberries is a phytoalexin with antioxidant properties. PTS exerts strong cytoprotective effects on various cells via Nuclear Factor Erythroid 2 like 2 (NFE2L2) pathway. We evaluated the antioxidant PTS treatment in mouse preimplantation embryos. In vitro culture media were supplemented with different concentrations of PTS. Treatment of zygotes with 0.25 μM PTS improved the development of day 4 blastocysts (P < 0.05). Moreover, H2 O2 treatment significantly increased the reactive oxygen species level and reduced the glutathione level in mouse blastocyst, whereas PTS treatment counteracted these effects...
November 15, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/30416150/ptbp1-contributes-to-spermatogenesis-through-regulation-of-proliferation-in-spermatogonia
#12
Manami Senoo, Takashi Takijiri, Nobuaki Yoshida, Manabu Ozawa, Masahito Ikawa
Polypyrimidine tract-binding protein 1 (PTBP1) is a highly conserved RNA-binding protein that is a well-known regulator of alternative splicing. Testicular tissue is one of the richest tissues with respect to the number of alternative splicing mRNA isoforms, but the molecular role(s) of PTBP1 in the regulation of these isoforms during spermatogenesis is still unclear. Here, we developed a germ cell-specific Ptbp1 conditional knockout (cKO) mouse model by using the Cre-loxP system to investigate the role of PTBP1 in spermatogenesis...
November 12, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/30393273/luteal-blood-flow-measured-by-doppler-ultrasonography-during-the-first-three-weeks-after-artificial-insemination-in-pregnant-and-non-pregnant-bos-indicus-dairy-cows
#13
Mubbashar Hassan, Usman Arshad, Muhammad Bilal, Abdul Sattar, Muhammad Avais, Heinrich Bollwein, Nasim Ahmad
The objective of this study was to determine if there are differences in luteal size (LS), progesterone (P4), and luteal blood flow (LBF) between pregnant and non-pregnant Bos indicus dairy cows during the first three weeks after insemination, and whether these parameters are related to each other. Lactating cows (n = 13) of mixed parity with a body weight of 430 ± 18 kg (mean ± SD), showing regular estrous cycle were used in the study. All cows were artificially inseminated and were classified as pregnant (embryonic heartbeat on day 30; n = 8) or non-pregnant (inter-estrus interval 17 to 21 days, n = 5)...
November 4, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/30393247/automated-detection-of-estrous-behavior-in-tie-stall-housing-using-a-barometer-and-accelerometer
#14
Hisashi Nabenishi, Shunsei Kawakami, Shota Shimo, Kaito Takeshita, Atusi Yamazaki, Kazuhiro Suzuki
Tie-stall housing inhibits movement in cows, thereby restricting the behavioral indicators used by farmers for detecting estrous. In this study, we investigated the changes in patterns of lying and standing times at estrous, and evaluated the potential for automated detection of estrous within tie-stalls using a barometer and accelerometer. On estrous days, total daily standing time was significantly longer than that during non-estrous days (P < 0.05). A practical method was developed for detecting slight altitude changes using a novel device, which consisted of a barometer and accelerometer, and was attached to the neckband...
November 3, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/30333376/neonatal-genistein-exposure-disrupts-ovarian-and-uterine-development-in-the-mouse-by-inhibiting-cellular-proliferation
#15
Guoyun Wu, Quanwei Wei, Debing Yu, Fangxiong Shi
Soy-based formula contains high concentrations of the isoflavone genistein. Genistein possesses estrogenic and tyrosine kinase inhibitory activity and interferes with cellular proliferation and development. To date, the acute and chronic effects of genistein on ovarian and uterine development have not been fully elucidated. In this study, mice at postnatal day 1 were subcutaneously injected with 100 mg/kg genistein for 10 consecutive days, and then their ovaries and uteri were collected on days 10, 21, and 90...
October 16, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/30318498/extracellular-glucose-levels-in-cultures-of-undifferentiated-mouse-trophoblast-stem-cells-affect-gene-expression-during-subsequent-differentiation-with-replicable-cell-line-dependent-variation
#16
Kenta Nishitani, Koji Hayakawa, Satoshi Tanaka
Mouse trophoblast stem cells (TSCs) have been established and maintained using hyperglycemic conditions (11 mM glucose) for no apparent good reason. Because glucose metabolites are used as resources for cellular energy production, biosynthesis, and epigenetic modifications, differences in extracellular glucose levels may widely affect cellular function. Since the hyperglycemic culture conditions used for TSC culture have not been fully validated, the effect of extracellular glucose levels on the properties of TSCs remains unclear...
October 13, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/30101829/comparison-of-the-microdrop-and-minimum-volume-cooling-methods-for-vitrification-of-porcine-in-vitro-produced-zygotes-and-blastocysts-after-equilibration-in-low-concentrations-of-cryoprotectant-agents
#17
Van Khanh Nguyen, Huong Thi Thu Vu, Huong Thi Nguyen, Huu Xuan Quan, Lan Doan Pham, Kazuhiro Kikuchi, Son Thanh Nguyen, Tamas Somfai
We compared the efficacy of the microdrop and minimum volume cooling (MVC) methods for the vitrification of in vitro-produced porcine zygotes and blastocysts after equilibration in low concentrations of cryoprotectant agents. Zygotes and blastocysts were equilibrated in 2% (v/v) ethylene glycol and 2% (v/v) propylene glycol for 13-15 min. Then, they were vitrified in a medium comprised of 17.5% ethylene glycol, 17.5% propylene glycol, 0.3 M sucrose, and 50 mg/ml polyvinylpyrrolidone either by either dropping them directly into liquid nitrogen (microdrop method) or placing them on Cryotop sheets in a minimum volume of medium and plunging into liquid nitrogen (MVC method)...
October 12, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/30078833/effects-of-recombinant-ovgp1-protein-on-in-vitro-bovine-embryo-development
#18
Blanca Algarra, Verónica Maillo, Manuel Avilés, Alfonso Gutiérrez-Adán, Dimitrios Rizos, María Jiménez-Movilla
Previously, our group demonstrated that recombinant porcine oviductin (pOVGP1) binds to the zona pellucida (ZP) of in vitro-matured (IVM) porcine oocytes with a positive effect on in vitro fertilization (IVF). The fact that pOVGP1 was detected inside IVM oocytes suggested that this protein had a biological role during embryo development. The aim of this study was to evaluate the effects of pOVGP1 on bovine in vitro embryo development. We applied 10 or 50 µg/ml of pOVGP1 during IVF, embryonic in vitro culture (IVC), or both, to evaluate cleavage and embryo development...
October 12, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/30033985/gdf9-and-bmp15-induce-development-of-antrum-like-structures-by-bovine-granulosa-cells-without-oocytes
#19
Md Hasanur Alam, Jibak Lee, Takashi Miyano
The role of oocytes in follicular antrum formation is not well understood. We examined the effect of oocyte-derived growth factors, growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15), on the formation of antrum-like structures by cultured bovine oocyte-granulosa cell complexes (OGCs). OGCs containing growing oocytes (105‒115 µm in diameter) were collected from early antral follicles (1.2‒1.8 mm) and used to prepare oocytectomized complexes (OXCs) and granulosa cell complexes (GCs)...
October 12, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/30033984/localization-of-sox2-positive-stem-progenitor-cells-in-the-anterior-lobe-of-the-common-marmoset-callithrix-jacchus-pituitary
#20
Saishu Yoshida, Ken Fujiwara, Takashi Inoue, Erika Sasaki, Yoshie Kametani, Susumu Takekoshi, Naoko Inoshita, Takako Kato, Yukio Kato
Studies on mouse and rat pituitaries reported that Sox2-expressing cells play roles as stem/progenitor cells in the adult pituitary gland. The presence of cells with stem cell-like properties in the pituitary adenoma and SOX2-positive cells has been demonstrated in the human pituitary. However, considering the difficulty in fully examining the stem/progenitor cell properties in the human pituitary, in the present study, we analyzed the SOX2-positive cells in the pituitary of the adult common marmoset (Callithrix jacchus), which is used as a non-human primate model...
October 12, 2018: Journal of Reproduction and Development
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