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Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopía Electronica ... Et. Al

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https://www.readbyqxmd.com/read/24432464/molecular-mechanisms-in-cell-signaling-and-gene-expression-abstracts-november-6-7-2013-buenos-aires-argentina
#1
(no author information available yet)
No abstract text is available yet for this article.
November 2013: Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopía Electronica ... Et. Al
https://www.readbyqxmd.com/read/24392581/autophagy-apoptosis-and-organelle-features-during-cell-exposure-to-cadmium
#2
Cristiane dos Santos Vergilio, Edésio José Tenório de Melo
Cadmium (Cd) induces several effects in different tissues, but our knowledge of the toxic effects on organelles is insufficient. To observe the progression of Cd effects on organelle structure and function, HuH-7 cells (human hepatic carcinoma cell line) were exposed to CdCl2 in increasing concentrations (1 microM - 20 microM) and exposure times (2 h - 24 h). During Cd treatment, the cells exhibited a progressive decrease in viability that was both time- and dose-dependent. Cd treated cells displayed progressive morphological changes that included cytoplasm retraction and nuclear condensation preceding a total loss of cell adhesion...
August 2013: Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopía Electronica ... Et. Al
https://www.readbyqxmd.com/read/24392580/creatine-supplementation-in-trained-rats-causes-changes-in-myenteric-neurons-and-intestinal-wall-morphometry
#3
Solange Marta Franzói De Moraes, Thais Andréia Brogio, Jacqueline Nelisis Zanoni, Mariana Cristina Vicente Umada Zapater, Sidney Barnabé Peres, Luzmarina Hernandes
Creatine is widely used by athletes as an ergogenic resource. The aim of this study was to evaluate the influence of creatine supplementation on the duodenum of rats submitted to physical training. The number and myenteric neuronal cell bodies as well mucosal and muscular tunic morphometry were evaluated. Control animals received a standard chow for 8 weeks, and the treated ones received the standard chow for 4 weeks and were later fed with the same chow but added with 2% creatine. Animals were divided in groups: sedentary, sedentary supplemented with creatine, trained and trained supplemented with creatine...
August 2013: Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopía Electronica ... Et. Al
https://www.readbyqxmd.com/read/24392579/confocal-laser-scanning-microscopy-and-immunohistochemistry-of-cerebellar-lugaro-cells
#4
Orlando J Castejón
The present paper shows by means of confocal laser scanning microscopy the immunoreactivity of rat cerebellar Lugaro cells for calbindin, synapsin-I, PSD-95, GluR1, CaMKII alpha, and N-cadherin. Lugaro cells were easily characterized by their location beneath Purkinje cells. Calbindin revealed immunoreactivity in the cell body, and the axonal and dendritic processes. Synapsin-I labelled the presynaptic endings on Lugaro cells. Synapsin-I and PSD-95 immunoreactivity demonstrated the localization of presynaptic and postsynaptic endings surrounding cell soma, corresponding to afferent extrinsic and intrinsic cerebellar fibers...
August 2013: Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopía Electronica ... Et. Al
https://www.readbyqxmd.com/read/24392578/post-treatment-with-plant-extracts-used-in-brazilian-folk-medicine-caused-a-partial-reversal-of-the-antiproliferative-effect-of-glyphosate-in-the-allium-cepa-test
#5
Viviane Dal-Souto Frescura, Andrielle Wouters Kuhn, Haywood Dail Laughinghouse, Juçara Terezinha Paranhos, Solange Bosio Tedesco
Species of the genus Psychotria are used for multiple purposes in Brazilian folk medicine, either as water infusions, baths or poultices. This study was aimed to evaluate the genotoxic and antiproliferative effects of infusions of Psychotria brachypoda and P. birotula on the Allium cepa test. Exposure to distilled water was used as a negative control, while exposure to glyphosate was used as a positive control. The interaction of extracts (as a post-treatment) with the effects of glyphosate was also studied...
August 2013: Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopía Electronica ... Et. Al
https://www.readbyqxmd.com/read/24392577/age-related-interaction-of-dopamine-and-serotonin-synthesis-in-striatal-synaptosomes
#6
Manoochehr Messripour, Azadeh Mesripour
Tyrosine hydroxylase and tryptophan hydroxylase are key rate limiting enzymes in the biosynthesis of dopamine and serotonin, respectively. Since both enzymes are active in striatum, and affected by age, this study was undertaken to investigate interaction between dopamine and serotonin synthesis in brain striatal synaptosomes of aging rat. Male Wistar rats (3 and 30 month old) were killed by decapitation and brain striatal synaptosomes were prepared by discontinuous Ficoll/sucrose gradient technique. Synaptosomes were incubated in the presence of added pargiline (monoamineoxidase inhibitor), dopamine or serotonin synthesized during 25 min was measured by HPLC, employing electrochemical detection...
August 2013: Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopía Electronica ... Et. Al
https://www.readbyqxmd.com/read/24396997/meta-analysis-of-the-cell-cycle-related-c12orf48
#7
Lokman Varisli
The cell cycle is a conserved process from yeast to mammals and focuses on mechanisms that regulate the timing and frequency of DNA replication and cell division. The temporal and spatial expression of the genes is tightly regulated to ensure accurate replication and transmission of DNA to daughter cells during the cycle. Although the genes involved in interphase are well studied, most of the genes which are involved in mitotic events still remain unidentified. Since, the discovery of mitosis related genes is still incomplete, we performed a co-expression and gene ontology analysis for revealing novel mitosis regulated genes...
April 2013: Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopía Electronica ... Et. Al
https://www.readbyqxmd.com/read/24396996/high-passage-numbers-induce-resistance-to-apoptosis-in-c2c12-muscle-cells
#8
Lucia Pronsato, Anabela La Colla, Ana Carolina Ronda, Lorena Milanesi, Ricardo Boland, Andrea Vasconsuelo
Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (< 20) or high (> 60) passage numbers (identified as 1-C2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H2O2 induction of apoptosis...
April 2013: Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopía Electronica ... Et. Al
https://www.readbyqxmd.com/read/23682430/pollen-viability-of-polygala-paniculata-l-polygalaceae-using-different-staining-methods
#9
Viviane Dal-Souto Frescura, Haywood Dail Laughinghouse, Thais Scotti do Canto-Dorow, Solange Bosio Tedesco
Polygala paniculata L. is a medicinal plant that grows in the Brazilian Atlantic coast, known as 'barba-de-São-João', 'barba-de-bode', 'vassourinha branca', and 'mimosa'. In this study, pollen viability was estimated by three different staining methods: 2% acetic orcein, 2% acetic carmine, and Alexander's stain. The young inflorescences of twenty accessions were collected and fixed in a solution of ethanol: acetic acid (3:1) for 24 hours, then stored in ethanol 70% under refrigeration. Six slides per plant, two for each stain, were prepared by squashing, and 300 pollen grains per slide were analyzed...
December 2012: Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopía Electronica ... Et. Al
https://www.readbyqxmd.com/read/23682429/structural-analysis-of-flagellar-axonemes-from-inner-arm-dynein-knockdown-strains-of-trypanosoma-brucei
#10
Randi Zukas, Alex J Chang, Marian Rice, Amy L Springer
Trypanosoma brucei is a protozoan flagellate that causes African sleeping sickness. Flagellar function in this organism is critical for life cycle progression and pathogenesis, however the regulation of flagellar motility is not well understood. The flagellar axoneme produces a complex beat through the precisely coordinated firing of many proteins, including multiple dynein motors. These motors are found in the inner arm and outer arm complexes. We are studying one of the inner arm dynein motors in the T. brucei flagellum: dynein-f...
December 2012: Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopía Electronica ... Et. Al
https://www.readbyqxmd.com/read/23682428/a-likely-role-for-a-novel-ph-domain-containing-protein-pepp2-in-connecting-membrane-and-cytoskeleton
#11
Yi Zou, Wenping Zhong
PH domains (pleckstrin homology) are well known to bind membrane phosphoinositides with different specificities and direct PH domain-containing proteins to discrete subcellular apartments with assistances of alternative binding partners. PH domain-containing proteins are found to be involved in a wide range of cellular events, including signalling, cytoskeleton rearrangement and vesicular trafficking. Here we showed that a novel PH domain-containing protein, PEPP2, displayed moderate phosphoinositide binding specificity...
December 2012: Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopía Electronica ... Et. Al
https://www.readbyqxmd.com/read/23682427/knockdown-of-apoptosis-inducing-factor-disrupts-function-of-respiratory-complex-i
#12
Miroslav Varecha, Daniela Páclová, Jirina Procházková, Pavel Matula, Dusan Cmarko, Michal Kozubek
Recent findings suggest that apoptotic protein apoptosis-inducing factor (AIF) may also play an important non-apoptotic function inside mitochondria. AIF was proposed to be an important component of respiratory chain complex I that is the major producer of superoxide radical. The possible role of AIF is still controversial. Superoxide production could be used as a valuable measure of complex I function, because the majority of superoxide is produced there. Therefore, we employed superoxide-specific mitochondrial fluorescence dye for detection of superoxide production...
December 2012: Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopía Electronica ... Et. Al
https://www.readbyqxmd.com/read/23682426/mechanisms-involved-in-the-cytotoxic-effects-of-berberine-on-human-colon-cancer-hct-8-cells
#13
Li-Na Xu, Bi-Nan Lu, Ming-Ming Hu, You-Wei Xu, Xu Han, Yan Qi, Jin-Yong Peng
Berberine, a constituent of some traditional Chinese medicinal plants, has been reported to have cytotoxicity effects on different human cancer cell lines. There is no available information about the effects and mechanism of action of berberine on human colon cancer cell line HCT-8. In this paper, the cytotoxicity ofberberine on HCT-8 cancer cells was investigated by MTT assay, fluorescence microscopy and flow cytometry analysis. Our data revealed that berberine could significantly inhibit the growth of HCT-8 cells in a dose- and time-dependent manner...
December 2012: Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopía Electronica ... Et. Al
https://www.readbyqxmd.com/read/23682425/reproductive-performance-of-the-mesa-silverside-chirostoma-jordani-woolman-1894-under-natural-and-controlled-photoperiods
#14
José Luis Arredondo-Figueroa, Laura Georgina Núñez-García, Paloma Adriana Heredia-Guzmán, Jesús T Ponce-Palafox
Chirostoma jordani is a native annual species inhabiting lacustrine waters of the Central Mexico Plateau. It is widely distributed and is currently facing high environmental pressures. Five experiments were performed to study the reproductive performance of this species. Four of the experiments were conducted in 270-L indoor recirculation tanks. Two males and one female at the first stage of reproduction were included in each test. A photoperiod of 14 light hours and 10 dark hours was used. In a fifth experiment, 10 females and 15 males were kept in an outdoor 3,000-L recirculation tank under natural photoperiod...
December 2012: Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopía Electronica ... Et. Al
https://www.readbyqxmd.com/read/23682424/effects-of-rotational-culture-on-morphology-nitric-oxide-production-and-cell-cycle-of-endothelial-cells
#15
Chaojun Tang, Xue Wu, Linqi Ye, Xiang Xie, Guixue Wang
Devices for the rotational culture of cells and the study of biological reactions have been widely applied in tissue engineering. However, there are few reports exploring the effects of rotational culture on cell morphology, nitric oxide (NO) production, and cell cycle of the endothelial cells from human umbilical vein on the stent surface. This study focuses on these parameters after the cells are seeded on the stents. Results showed that covering of stents by endothelial cells was improved by rotational culture...
December 2012: Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopía Electronica ... Et. Al
https://www.readbyqxmd.com/read/23577362/abstracts-of-the-48th-annual-meeting-of-the-argentine-society-for-biochemistry-and-molecular-biology-october-29-november-1-2012-mendoza-argentina
#16
(no author information available yet)
No abstract text is available yet for this article.
October 2012: Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopía Electronica ... Et. Al
https://www.readbyqxmd.com/read/23185784/modulation-of-il-10-il-10r-expression-by-mafosfamide-a-derivative-of-4-hydroxycyclophosphamide-in-a-rat-b-cell-lymphoma
#17
COMPARATIVE STUDY
Maria J Rico, Pablo Matar, O Graciela Scharovsky
We have already shown that IL-10 plays an important role in immunosuppression and metastatic dissemination in the rat B-cell lymphoma L-TACB model. It was suggested that the up-regulation of IL-10 production and IL-10 receptor (IL-10R) expression would be part of the transition from primary tumor to metastatic phenotype and that IL-10, besides its immunosuppressive activity, may act as a growth factor for metastatic L-TACB cells. The treatment of L-TACB-bearing rats with a single low-dose cyclophosphamide decreased IL-10 production, reverted immunosuppression and induced the immunologic rejection of tumor metastasis without any effect on primary tumor growth...
August 2012: Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopía Electronica ... Et. Al
https://www.readbyqxmd.com/read/23185783/histological-analysis-of-pollen-pistil-interactions-in-sour-passion-fruit-plants-passiflora-edulis-sims
#18
Hérika Chagas Madureira, Telma Nair Santana Pereira, Maura Da Cunha, Denise Espellet Klein
The success of sexual plant reproduction is directly influenced by specific interactions between the pollen and pistil. Light, fluorescence and scanning electron microscopy techniques were used to evaluate the steps of pollination in sour passion fruit plants (Passiflora edulis Sims). In the compatible interaction, pollen tubes grow through stigma projections towards the ovary. The pollen grain surface was found to be spheroidal and to consist of heteroreticulate exine with six colpi. Furthermore, analysis in vivo of pollen-pistil interactions indicated that stigmas of flowers 24 hours before anthesis are unable to discriminate compatible (genetically unrelated) and incompatible (genetically related) pollen grains...
August 2012: Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopía Electronica ... Et. Al
https://www.readbyqxmd.com/read/23185782/human-umbilical-artery-smooth-muscle-exhibits-a-2-apb-sensitive-capacitative-contractile-response-evoked-by-vasoactive-substances-and-expresses-mrnas-for-stim-orai-and-trpc-channels
#19
Ana Rocio Roldán Palomo, Pedro Martín, Alejandro Rebolledo, Nicolás Enrique, Luis E Flores, Verónica Milesi
After depletion of intracellular Ca2+ stores the capacitative response triggers an extracellular Ca2+ influx through store-operated channels (SOCs) which refills these stores. Our objective was to explore if human umbilical artery smooth muscle presented this response and if it was involved in the mechanism of serotonin- and histamine-induced contractions. Intracellular Ca2+ depletion by a Ca(2+)-free extracellular solution followed by Ca2+ readdition produced a contraction in artery rings which was inhibited by the blocker of Orai and TRPC channels 2-aminoethoxydiphenyl borate (2-APB), suggesting a capacitative response...
August 2012: Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopía Electronica ... Et. Al
https://www.readbyqxmd.com/read/23185781/butein-imparts-free-radical-scavenging-anti-oxidative-and-proapoptotic-properties-in-the-flower-extracts-of-butea-monosperma
#20
COMPARATIVE STUDY
Anuradha Sehrawat, Vijay Kumar
The flower of Butea monosperma (Lam.) (Fabaceae) has been used in traditional Indian medicine in the treatment of many ailments including liver disorders. To understand the pharmacological basis of its beneficial effects, the extracts of dried flowers in water, methanol, butanol, ethyl acetate and acetone were evaluated for free radical scavenging and pro-apoptotic activities in cell cultures (human hepatoma Huh-7 cell line and immortalized AML-12 mouse hepatocytes). Butrin and butein -the active constituents of flower extracts- were used as reference molecules...
August 2012: Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopía Electronica ... Et. Al
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