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Microbiological Research

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https://www.readbyqxmd.com/read/28647125/isogenic-mutations-in-the-moraxella-catarrhalis-cyddc-system-display-pleiotropic-phenotypes-and-reveal-the-role-of-a-palindrome-sequence-in-its-transcriptional-regulation
#1
Yosra I Nagy, Manal M M Hussein, Yasser M Ragab, Ahmed S Attia
Moraxella catarrhalis is becoming an important human respiratory tract pathogen affecting significant proportions from the population. However, still little is known about its physiology and molecular regulation. To this end, the CydDC, which is a heterodimeric ATP binding cassette transporter that has been shown to contribute to the maintenance of the redox homeostasis across the periplasm in other Gram-negative bacteria, is studied here. Amino acids multiple sequence alignments indicated that M. catarrhalis CydC is different from the CydC proteins of the bacterial species in which this system has been previously studied...
September 2017: Microbiological Research
https://www.readbyqxmd.com/read/28647124/putative-protein-vc0395_0300-from-vibrio-cholerae-is-a-diguanylate-cyclase-with-a-role-in-biofilm-formation
#2
Divya Bandekar, Om Prakash Chouhan, Swati Mohapatra, Mousumi Hazra, Saugata Hazra, Sumit Biswas
The hallmark of the lifecycle of Vibrio cholerae is its ability to switch between two lifestyles - the sessile, non-pathogenic form and the motile, infectious form in human hosts. One of these changes is in the formation of surface biofilms, when in sessile aquatic habitats. The cell-cell interactions within a V. cholerae biofilm are stabilized by the production of an exopolysachharide (EPS) matrix, which in turn is regulated by the ubiquitous secondary messenger, cyclic di-GMP (c-di-GMP), synthesized by proteins containing GGD(/E)EF domains in all prokaryotic systems...
September 2017: Microbiological Research
https://www.readbyqxmd.com/read/28647123/zinc-solubilizing-bacillus-spp-potential-candidates-for-biofortification-in-maize
#3
Muhammad Zahid Mumtaz, Maqshoof Ahmad, Moazzam Jamil, Tanveer Hussain
Bioaugmentation of Zn solubilizing rhizobacteria could be a sustainable intervention to increase bioavailability of Zn in soil which can be helpful in mitigation of yield loss and malnutrition of zinc. In present study, a number of pure rhizobacterial colonies were isolated from maize rhizosphere and screened for their ability to solubilize zinc oxide. These isolates were screened on the basis of zinc and phosphate solubilization, IAA production, protease production, catalase activity and starch hydrolysis...
September 2017: Microbiological Research
https://www.readbyqxmd.com/read/28647122/effect-of-succinate-on-phosphate-solubilization-in-nitrogen-fixing-bacteria-harbouring-chick-pea-and-their-effect-on-plant-growth
#4
Bhagya Iyer, Mahendrapal Singh Rajput, Shalini Rajkumar
Diverse nitrogen fixing bacteria harbouring chick pea rhizosphere and root nodules were tested for multiple plant growth promoting traits like tricalcium phosphate (TCP) and rock phosphate (RP) solubilization, production of ammonia, indole 3-acetic acid, chitinase, phytase and alkaline phosphatase. Isolates belonged to diverse genus like Enterobacter, Acinetobacter, Erwinia, Pseudomonas, Rhizobium, Sinorhizobium, Ensifer, Klebsiella, etc. Most isolates solubilized TCP and RP along with the lowering of media pH, indicating acidification to be the chief mechanism behind this solubilization...
September 2017: Microbiological Research
https://www.readbyqxmd.com/read/28647121/dna-based-stable-isotope-probing-identifies-formate-metabolizing-methanogenic-archaea-in-paddy-soil
#5
Jiangbing Xu, Zhongjun Jia, Xiangui Lin, Youzhi Feng
Paddy methane (CH4) production is biologically dominated by methanogenic archaea that metabolize a variety of organic and/or inorganic carbon sources. Though formate is easily dissimilated into H2/CO2, formate-metabolizing methanogenic archaea are distinct from CO2-utilizing methanogen taxa. The identity of formate-metabolizing methanogenic archaea in paddy soil remains elusive. In this investigation, molecular approaches based on stable isotope probing (SIP) technique were conducted to identify the formate-metabolizing methanogenic archaea in paddy soil...
September 2017: Microbiological Research
https://www.readbyqxmd.com/read/28647120/drrc-protein-of-streptomyces-peucetius-removes-daunorubicin-from-intercalated-dnri-promoter
#6
Francis Prija, Ranjan Prasad
DrrC is a DNA-binding protein of Streptomyces peucetius that provides self-resistance against daunorubicin, the antibiotic produced by the organism. DrrC was expressed in E.coli and purified by using N-terminal MBP-tag which retained DNA-binding property in spite of the tag. Mobility shift assay confirmed the interaction of 313bp DNA that has the dnrI promoter, daunorubicin and MBP-DrrC in the presence of ATP. Biotinylated and immobilized 313bp DNA was intercalated with daunorubicin to observe the release of the drug when MBP-DrrC is allowed to act on the DNA...
September 2017: Microbiological Research
https://www.readbyqxmd.com/read/28647119/in-vitro-pgpr-properties-and-osmotic-tolerance-of-different-azospirillum-native-strains-and-their-effects-on-growth-of-maize-under-drought-stress
#7
Julia E García, Guillermo Maroniche, Cecilia Creus, Ramón Suárez-Rodríguez, José Augusto Ramirez-Trujillo, María D Groppa
Osmotic variations in the soil can affect bacterial growth diminishing the number of inoculated bacteria. In a scenario of water deficit having tolerant bacteria would be beneficial to achieve a better response of the plant to stress. Thus, selection of more resistant bacteria could be useful to design new inoculants to be used in arid zones. In this sense, a group of Azospirillum isolates deposited in INTA collection was characterized in order to select strains tolerant to osmotic stress. The results obtained demonstrated that Az19 strain has similar in vitro PGPR characteristics to Az39, the most used strain in Argentina for inoculants industries, with the advantage of a better tolerance to osmotic and salt stress...
September 2017: Microbiological Research
https://www.readbyqxmd.com/read/28647118/screening-of-wheat-endophytes-as-biological-control-agents-against-fusarium-head-blight-using-two-different-in-vitro-tests
#8
Morgane Comby, Marie Gacoin, Mathilde Robineau, Fanja Rabenoelina, Sébastien Ptas, Joëlle Dupont, Camille Profizi, Fabienne Baillieul
In order to find biological control agents (BCAs) for the management of Fusarium head blight (FHB), a major disease on wheat crops worldwide, 86 microorganisms isolated from inner tissues of wheat plants were discriminated for their ability to inhibit the growth of Fusarium graminearum and Fusarium culmorum by in vitro dual culture assays. A group of 22 strains appeared very effective to inhibit F. graminearum (inhibition of 30-51%) and they were also globally effective in controlling F. culmorum (inhibition of 15-53%)...
September 2017: Microbiological Research
https://www.readbyqxmd.com/read/28647117/isolation-of-a-potential-biocontrol-agent-paenibacillus-polymyxa-nsy50-from-vinegar-waste-compost-and-its-induction-of-host-defense-responses-against-fusarium-wilt-of-cucumber
#9
Nanshan Du, Lu Shi, Yinghui Yuan, Jin Sun, Sheng Shu, Shirong Guo
Fusarium wilt caused by Fusarium oxysporum f. sp. cucumerinum (FOC) is one of the major destructive soil-borne diseases infecting cucumber. In this study, we screened 60 target strains isolated from vinegar waste compost, from which 10 antagonistic strains were identified to have the disease suppression capacity of bio-control agents. The 16S rDNA gene demonstrated that the biocontrol agents were Paenibacillus polymyxa (P. polymyxa), Bacillus amyloliquefaciens (B. amyloliquefaciens) and Bacillus licheniformis (B...
September 2017: Microbiological Research
https://www.readbyqxmd.com/read/28602403/panacea-in-progress-crispr-and-the-future-of-its-biological-research-introduction
#10
REVIEW
Michael Carroll, Xiaohui Zhou
The elucidation of the CRISPR (clustered, regularly interspaced, short palindromic repeats) adaptive immune system endogenous to most microbial life has culminated in progress in a diversity of scientific disciplines. The concurrently promising and eccentric nature of its theoretically plausible applications has wrought enthusiasm in the research community globally, potentiating advancements in human and animal health, ecological stability, and economic wellbeing, that would hitherto be considered the unattainable fancies of a futurist...
August 2017: Microbiological Research
https://www.readbyqxmd.com/read/28602402/growth-media-affect-the-volatilome-and-antimicrobial-activity-against-phytophthora-infestans-in-four-lysobacter-type-strains
#11
Valentina Lazazzara, Michele Perazzolli, Ilaria Pertot, Franco Biasioli, Gerardo Puopolo, Luca Cappellin
Bacterial volatile organic compounds (VOCs) play important ecological roles in soil microbial interactions. Lysobacter spp. are key determinants of soil suppressiveness against phytopathogens and the production of non-volatile antimicrobial metabolites has been extensively characterised. However, the chemical composition and antagonistic properties of the Lysobacter volatilome have been poorly investigated. In this work, VOC emission profiles of four Lysobacter type strains grown on a sugar-rich and a protein-rich medium were analysed using solid-phase microextraction gas chromatography-mass spectrometry and proton transfer reaction-time of flight-mass spectrometry...
August 2017: Microbiological Research
https://www.readbyqxmd.com/read/28602401/fusarium-infection-causes-genotoxic-disorders-and-antioxidant-based-damages-in-orobanche-spp
#12
Mehmet Aybeke
This study aims to evaluate the toxic effects of Fusarium oxysporum on root parasitic weed, Orobanche spp. Comparative genetic and gene expression studies were conducted on uninfected and fungus-infected orobanches. In genetic studies, isolated total DNA was amplified by RAPD PCR. Fragment properties were analysed by GTS test. According to the results, the fragment properties of control and Fusarium infected (experimental) groups varied widely; and it has been observed that Fusarium has genotoxic effects on the DNA of orobanches...
August 2017: Microbiological Research
https://www.readbyqxmd.com/read/28602400/novel-protein-interactions-with-an-actin-homolog-mreb-of-helicobacter-pylori-determined-by-bacterial-two-hybrid-system
#13
Reyna Cristina Zepeda Gurrola, Yajuan Fu, Isabel Cristina Rodríguez Luna, Claudia Guadalupe Benítez Cardoza, María de Jesús López López, Yolanda López Vidal, Germán Rubén Aguilar Gutíerrez, Mario A Rodríguez Pérez, Xianwu Guo
The bacterium Helicobacter pylori infects more than 50% of the world population and causes several gastroduodenal diseases, including gastric cancer. Nevertheless, we still need to explore some protein interactions that may be involved in pathogenesis. MreB, an actin homolog, showed some special characteristics in previous studies, indicating that it could have different functions. Protein functions could be realized via protein-protein interactions. In the present study, the MreB protein from H. pylori 26695 fused with two tags 10×His and GST in tandem was overexpressed and purified from Escherchia coli...
August 2017: Microbiological Research
https://www.readbyqxmd.com/read/28602399/stpbs2-a-mapk-kinase-gene-is-involved-in-determining-hyphal-morphology-cell-wall-development-hypertonic-stress-reaction-as-well-as-the-production-of-secondary-metabolites-in-northern-corn-leaf-blight-pathogen-setosphaeria-turcica
#14
Xiao-Dong Gong, Sheng-Ze Feng, Jie Zhao, Cong Tang, Lan Tian, Yong-Shan Fan, Zhi-Yan Cao, Zhi-Min Hao, Hui Jia, Jin-Ping Zang, Yun-Feng Zhang, Jian-Min Han, Shou-Qin Gu, Jin-Gao Dong
Mitogen activated protein kinase kinase (MAPKK) is a crucial component in the MAPK signaling pathway. However, the functions of MAPKKs in foliar pathogens remain poorly understood. In the current study, a MAPKK gene designated as StPBS2 was cloned from Setosphaeria turcica and the functions of this gene were investigated by RNAi technology. Four independent StPBS2 gene silence transformants with different efficiencies were confirmed by real time PCR. Compared to the wild type strain (WT), these transformants showed decreased colony growth, shortened hyphae cell length, broadened cell width and an obvious reduction in conidium yield...
August 2017: Microbiological Research
https://www.readbyqxmd.com/read/28602398/in-vitro-and-in-vivo-antifungal-efficacy-of-plant-based-lawsone-against-fusarium-oxysporum-species-complex
#15
S H S Dananjaya, R M C Udayangani, Sang Yeop Shin, M Edussuriya, Chamilani Nikapitiya, Jehee Lee, Mahanama De Zoysa
Fusarium oxysporum is an ascomycete facultative fungus which generally affects to plants. However, it is recently known as a serious emerging opportunistic pathogen of human and other animals. F. oxysporum shows broad resistance to commonly used antifungal agents and therefore development of alternative therapeutic agents is required. In this study, we investigated the antifungal efficacy of plant based natural lawsone against pathogenic F. oxysporum. Antifungal susceptibility test determined the concentration dependent growth inhibition of lawsone against F...
August 2017: Microbiological Research
https://www.readbyqxmd.com/read/28602397/a-new-species-of-scopulariopsis-and-its-synergistic-effect-on-pathogenicity-of-verticillium-dahliae-on-cotton-plants
#16
Xiao-Lin Li, Mohammad Reza Ojaghian, Jing-Ze Zhang, Shui-Jin Zhu
A new species, Scopulariopsis gossypii, was found to be present in the vascular bundles of cotton plants (Gossypium hirsutum) infected by Verticillium dahliae which is an economically important pathogen in Hangzhou, China. The fungus was only present in the diseased plants, but it never became isolated from the healthy plants. Scanning electron microscopy showed that the fungus was present in parenchyma cells enclosing vessels in dark brown vascular tissues of stems, and produced asexual conidia within the tissues...
August 2017: Microbiological Research
https://www.readbyqxmd.com/read/28602396/mycoplasma-fermentans-deacetylase-promotes-mammalian-cell-stress-tolerance
#17
Qingzhou Cheng, Lijuan Wu, Rongfu Tu, Jun Wu, Wenqian Kang, Tong Su, Runlei Du, Wenbin Liu
Mycoplasma fermentans is a pathogenic bacterium that infects humans and has potential pathogenic roles in respiratory, genital and rheumatoid diseases. NAD(+)-dependent deacetylase is involved in a wide range of pathophysiological processes and our studies have demonstrated that expression of mycoplasmal deacetylase in mammalian cells inhibits proliferation but promotes anti-starvation stress tolerance. Furthermore, mycoplasmal deacetylase is involved in cellular anti-oxidation, which correlates with changes in the proapoptotic proteins BIK, p21 and BIM...
August 2017: Microbiological Research
https://www.readbyqxmd.com/read/28527765/lignocellulosic-sugar-management-for-xylitol-and-ethanol-fermentation-with-multiple-cell-recycling-by-kluyveromyces-marxianus-iipe453
#18
Diptarka Dasgupta, Debashish Ghosh, Sheetal Bandhu, Dilip K Adhikari
Optimum utilization of fermentable sugars from lignocellulosic biomass to deliver multiple products under biorefinery concept has been reported in this work. Alcohol fermentation has been carried out with multiple cell recycling of Kluyveromyces marxianus IIPE453. The yeast utilized xylose-rich fraction from acid and steam treated biomass for cell generation and xylitol production with an average yield of 0.315±0.01g/g while the entire glucose rich saccharified fraction had been fermented to ethanol with high productivity of 0...
July 2017: Microbiological Research
https://www.readbyqxmd.com/read/28527764/differential-stress-response-of-saccharomyces-hybrids-revealed-by-monitoring-hsp104-aggregation-and-disaggregation
#19
Claudia Kempf, Klaus Lengeler, Jürgen Wendland
Proteotoxic stress may occur upon exposure of yeast cells to different stress conditions. The induction of stress response mechanisms is important for cells to adapt to changes in the environment and ensure survival. For example, during exposure to elevated temperatures the expression of heat shock proteins such as Hsp104 is induced in yeast. Hsp104 extracts misfolded proteins from aggregates to promote their refolding. We used an Hsp104-GFP reporter to analyze the stress profiles of Saccharomyces species hybrids...
July 2017: Microbiological Research
https://www.readbyqxmd.com/read/28527763/aluminium-resistant-plant-growth-promoting-bacteria-induce-overexpression-of-aluminium-stress-related-genes-in-arabidopsis-thaliana-and-increase-the-ginseng-tolerance-against-aluminium-stress
#20
Mohamed El-Agamy Farh, Yeon-Ju Kim, Johan Sukweenadhi, Priyanka Singh, Deok-Chun Yang
Panax ginseng is an important cash crop in the Asian countries due to its pharmaceutical effects, however the plant is exposed to various abiotic stresses, lead to reduction of its quality. One of them is the Aluminum (Al) accumulation. Plant growth promoting bacteria which able to tolerate heavy metals has been considered as a new trend for supporting the growth of many crops in heavy metal occupied areas. In this study, twelve bacteria strains were isolated from rhizosphere of diseased Korean ginseng roots located in Gochang province, Republic of Korea and tested for their ability to grow in Al-embedded broth media...
July 2017: Microbiological Research
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