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Microbiological Research

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https://www.readbyqxmd.com/read/28527765/lignocellulosic-sugar-management-for-xylitol-and-ethanol-fermentation-with-multiple-cell-recycling-by-kluyveromyces-marxianus-iipe453
#1
Diptarka Dasgupta, Debashish Ghosh, Sheetal Bandhu, Dilip K Adhikari
Optimum utilization of fermentable sugars from lignocellulosic biomass to deliver multiple products under biorefinery concept has been reported in this work. Alcohol fermentation has been carried out with multiple cell recycling of Kluyveromyces marxianus IIPE453. The yeast utilized xylose-rich fraction from acid and steam treated biomass for cell generation and xylitol production with an average yield of 0.315±0.01g/g while the entire glucose rich saccharified fraction had been fermented to ethanol with high productivity of 0...
July 2017: Microbiological Research
https://www.readbyqxmd.com/read/28527764/differential-stress-response-of-saccharomyces-hybrids-revealed-by-monitoring-hsp104-aggregation-and-disaggregation
#2
Claudia Kempf, Klaus Lengeler, Jürgen Wendland
Proteotoxic stress may occur upon exposure of yeast cells to different stress conditions. The induction of stress response mechanisms is important for cells to adapt to changes in the environment and ensure survival. For example, during exposure to elevated temperatures the expression of heat shock proteins such as Hsp104 is induced in yeast. Hsp104 extracts misfolded proteins from aggregates to promote their refolding. We used an Hsp104-GFP reporter to analyze the stress profiles of Saccharomyces species hybrids...
July 2017: Microbiological Research
https://www.readbyqxmd.com/read/28527763/aluminium-resistant-plant-growth-promoting-bacteria-induce-overexpression-of-aluminium-stress-related-genes-in-arabidopsis-thaliana-and-increase-the-ginseng-tolerance-against-aluminium-stress
#3
Mohamed El-Agamy Farh, Yeon-Ju Kim, Johan Sukweenadhi, Priyanka Singh, Deok-Chun Yang
Panax ginseng is an important cash crop in the Asian countries due to its pharmaceutical effects, however the plant is exposed to various abiotic stresses, lead to reduction of its quality. One of them is the Aluminum (Al) accumulation. Plant growth promoting bacteria which able to tolerate heavy metals has been considered as a new trend for supporting the growth of many crops in heavy metal occupied areas. In this study, twelve bacteria strains were isolated from rhizosphere of diseased Korean ginseng roots located in Gochang province, Republic of Korea and tested for their ability to grow in Al-embedded broth media...
July 2017: Microbiological Research
https://www.readbyqxmd.com/read/28527762/effects-of-rhamnolipids-on-microorganism-characteristics-and-applications-in-composting-a-review
#4
REVIEW
Binbin Shao, Zhifeng Liu, Hua Zhong, Guangming Zeng, Guansheng Liu, Mingda Yu, Yang Liu, Xin Yang, Zhigang Li, Zhendong Fang, Juntao Zhang, Chenghao Zhao
Biosurfactant rhmnolipids have been applied in many fields, especially in environmental bioremediation. According to previous researches, many research groups have studied the influence of rhamnolipids on microorganism characteristics and/or its application in composting. In this review, the effects of rhamnolipids on the cell surface properties of microorganisms was discussed firstly, such as cell surface hydrophobicity (CSH), electrical, surface compounds, etc. Moreover, the deeper mechanisms were also discussed, such as the effects of rhamnolipids on the structural characteristics and functional characteristics of the cell membrane, and the effects of rhamnolipids on the related enzymes and genes...
July 2017: Microbiological Research
https://www.readbyqxmd.com/read/28527761/bead-based-flow-cytometry-for-semi-quantitative-analysis-of-complex-membrane-vesicle-populations-released-by-bacteria-and-host-cells
#5
Charlotte Volgers, Birke J Benedikter, Gert E Grauls, Paul H M Savelkoul, Frank R M Stassen
During infection, the release of nano-sized membrane vesicle is a process which is common both for bacteria and host cells. Host cell-derived membrane vesicles can be involved in innate and adaptive immunity whereas bacterial membrane vesicles can contribute to bacterial pathogenicity. To study the contribution of both membrane vesicle populations during infection is highly complicated as most vesicles fall within a similar size range of 30-300nm. Specialized techniques for purification are required and often no single technique complies on its own...
July 2017: Microbiological Research
https://www.readbyqxmd.com/read/28527760/restoration-of-cefixime-induced-gut-microbiota-changes-by-lactobacillus-cocktails-and-fructooligosaccharides-in-a-mouse-model
#6
Ying Shi, Qixiao Zhai, Dongyao Li, Bingyong Mao, Xiaoming Liu, Jianxin Zhao, Hao Zhang, Wei Chen
Probiotics have been used to rebuild the antibiotic-induced dysfunction in gut microbiota, but whether the different strains of probiotics result in similar or reverse effects remains unclear. In this study, the different recovery effects of two cocktails (each contains four strains) of Lactobacillus and fructooligosaccharide against cefixime-induced change of gut microbiota were evaluated in C57BL/6J mice. The results show that the use of cefixime caused a reduction in the diversities of the microbial community and led to significantly decreasing to one preponderant Firmicutes phylum, which was difficult to restore naturally in the short term...
July 2017: Microbiological Research
https://www.readbyqxmd.com/read/28527759/selection-of-new-lactic-acid-bacteria-strains-bearing-probiotic-features-from-mucosal-microbiota-of-healthy-calves-looking-for-immunobiotics-through-in-vitro-and-in-vivo-approaches-for-immunoprophylaxis-applications
#7
Sávio Sandes, Luige Alvim, Bruno Silva, Leonardo Acurcio, Cinara Santos, Márcia Campos, Camila Santos, Jacques Nicoli, Elisabeth Neumann, Álvaro Nunes
From the birth, since their mucosal microbiota and immune system are not fully developed, newborn calves are susceptible to several mucosal pathogenic microorganisms. Operating through humoral and non-humoral mechanisms in the host, several lactic acid bacteria strains bearing probiotic features are often employed in livestock as food supplement, improving animal production performance, promoting health and reducing the severity of mucosal infections. Accordingly, we isolated, species-level identified and screened for their probiotic potentials seventy lactic acid bacteria strains from upper airway, vaginal and intestinal mucosa of healthy calves...
July 2017: Microbiological Research
https://www.readbyqxmd.com/read/28454714/growth-promotion-of-peanut-arachis-hypogaea-l-and-maize-zea-mays-l-plants-by-single-and-mixed-cultures-of-efficient-phosphate-solubilizing-bacteria-that-are-tolerant-to-abiotic-stress-and-pesticides
#8
María Soledad Anzuay, María Gabriela Ruiz Ciancio, Liliana Mercedes Ludueña, Jorge Guillermo Angelini, Germán Barros, Nicolás Pastor, Tania Taurian
The aims of this study were, to analyze in vitro phosphate solubilization activity of six native peanut bacteria and to determine the effect of single and mixed inoculation of these bacteria on peanut and maize plants. Ability to produce organic acids and cofactor PQQ, to solubilize FePO4 and AlPO4 and phosphatase activity were analyzed. Also, the ability to solubilize phosphate under abiotic stress and in the presence of pesticides of the selected bacteria was determined. The effect of single and mixed bacterial inocula was analyzed on seed germination, maize plant growth and in a crop rotation plant assay with peanut and maize...
June 2017: Microbiological Research
https://www.readbyqxmd.com/read/28454713/fengycin-produced-by-bacillus-subtilis-9407-plays-a-major-role-in-the-biocontrol-of-apple-ring-rot-disease
#9
Haiyan Fan, Jinjiang Ru, Yuanyuan Zhang, Qi Wang, Yan Li
Apple ring rot, caused by Botryosphaeria dothidea, is a serious apple disease in China. Bacillus subtilis 9407 was isolated from healthy apples and showed strong antifungal activity against B. dothidea. To identify the primary antifungal compound of B. subtilis 9407 and determine its role in controlling apple ring rot, a transposon mutant library was constructed using TnYLB-1, and a mutant completely defective in antifungal activity was obtained. The gene inactivated in the antifungal activity mutant had 98...
June 2017: Microbiological Research
https://www.readbyqxmd.com/read/28454712/transcriptome-based-analysis-of-the-saprophytic-fungus-abortiporus-biennis-response-to-oxalic-acid
#10
Marcin Grąz, Anna Jarosz-Wilkołazka, Grzegorz Janusz, Andrzej Mazur, Jerzy Wielbo, Piotr Koper, Kamil Żebracki, Agnieszka Kubik-Komar
In this study, the transcriptomic-based response of the white rot fungus Abortiporus biennis to oxalic acid induction was reported. The whole transcriptome of A. biennis was analysed using the RNA-based sequencing technology and Solid 5500 platform. De novo assembly of reads generated 37,719 contigs. A molecular function for 26,280 unique transcripts was assigned. The analysis of the A. biennis transcriptome predicted 635 hypothetical open reading frames encoding carbohydrate active enzymes distributed in 122 families...
June 2017: Microbiological Research
https://www.readbyqxmd.com/read/28454711/microbial-interference-mitigates-meloidogyne-incognita-mediated-oxidative-stress-and-augments-bacoside-content-in-bacopa-monnieri-l
#11
Rupali Gupta, Akanksha Singh, P V Ajayakumar, Rakesh Pandey
Microbial interference plays an imperative role in plant development and response to various stresses. However, its involvement in mitigation of oxidative stress generated by plant parasitic nematode in plants remains elusive. In the present investigation, the efficacy of microbe's viz., Chitiniphilus sp. MTN22 and Streptomyces sp. MTN14 single and in combinations was examined to mitigate oxidative stress generated by M. incognita in medicinal plant, Bacopa monnieri. Microbial combination with and without pathogen also enhanced the growth parameters along with secondary metabolites (bacoside) of B...
June 2017: Microbiological Research
https://www.readbyqxmd.com/read/28454710/identification-of-putative-effector-genes-and-their-transcripts-in-three-strains-related-to-candidatus-phytoplasma-aurantifolia
#12
Ameneh Anabestani, Keramat Izadpanah, Simona Abbà, Luciana Galetto, Abozar Ghorbani, Sabrina Palmano, Majid Siampour, Flavio Veratti, Cristina Marzachì
Molecular mechanisms underlying phytoplasma interactions with host plants are largely unknown. In this study attempts were made to identify effectors of three phytoplasma strains related to 'Ca. P. aurantifolia', crotalaria phyllody (CrP), faba bean phyllody (FBP), and witches' broom disease of lime (WBDL), using information from draft genome of peanut witches' broom phytoplasma. Seven putative effectors were identified in WBDL genome (SAP11, SAP21, Eff64, Eff115, Eff197, Eff211 and EffSAP67), five (SAP11, SAP21, Eff64, Eff99 and Eff197) in CrP and two (SAP11, Eff64) in FBP...
June 2017: Microbiological Research
https://www.readbyqxmd.com/read/28454709/involvement-of-phenazine-1-carboxylic-acid-in-the-interaction-between-pseudomonas-chlororaphis-subsp-aureofaciens-strain-m71-and-seiridium-cardinale-in-vivo
#13
Aida Raio, Pierluigi Reveglia, Gerardo Puopolo, Alessio Cimmino, Roberto Danti, Antonio Evidente
Pseudomonas chlororaphis subsp. aureofaciens encompasses bacterial strains that effectively control phytopathogenic fungi through the production of the natural antibiotics named phenazines. In this work, the involvement of phenazine production in the interaction between the biological control agent P. chlororaphis subsp. aureofaciens M71 and the fungus Seiridium cardinale, a serious cypress pathogen, was investigated. Field trials were carried out to assess the role of phenazines in the control of S. cardinale in vivo...
June 2017: Microbiological Research
https://www.readbyqxmd.com/read/28454708/functional-characterisation-of-burkholderia-pseudomallei-biotin-protein-ligase-a-toolkit-for-anti-melioidosis-drug-development
#14
Thomas E H Bond, Alanna E Sorenson, Patrick M Schaeffer
Burkholderia pseudomallei (Bp) is the causative agent of melioidosis. The bacterium is responsible for 20% of community-acquired sepsis cases and 40% of sepsis-related mortalities in northeast Thailand, and is intrinsically resistant to aminoglycosides, macrolides, rifamycins, cephalosporins, and nonureidopenicillins. There is no vaccine and its diagnosis is problematic. Biotin protein ligase (BirA) which is essential for fatty acid synthesis has been proposed as a drug target in bacteria. Very few bacterial BirA have been characterized, and a better understanding of these enzymes is necessary to further assess their value as drug targets...
June 2017: Microbiological Research
https://www.readbyqxmd.com/read/28454707/piriformospora-indica-promotes-growth-seed-yield-and-quality-of-brassica-napus-l
#15
Zhen-Zhu Su, Ting Wang, Neeraj Shrivastava, You-Yuan Chen, Xiaoxi Liu, Chao Sun, Yufeng Yin, Qi-Kang Gao, Bing-Gan Lou
In current scenario, crop productivity is being challenged by decreasing soil fertility. To cope up with this problem, different beneficial microbes are explored to increase the crop productivity with value additions. In this study, Brassica napus L., an important agricultural economic oilseed crop with rich source of nutritive qualities, was interacted with Piriformospora indica, a unique root colonizing fungus with wide host range and multifunctional aspects. The fungus-treated plants showed a significant increase in agronomic parameters with plant biomass, lodging-resistance, early bolting and flowering, oil yield and quality...
June 2017: Microbiological Research
https://www.readbyqxmd.com/read/28454706/microbial-diversity-of-the-hypersaline-and-lithium-rich-salar-de-uyuni-bolivia
#16
Götz Haferburg, Janosch A D Gröning, Nadja Schmidt, Nicolai-Alexeji Kummer, Juan Carlos Erquicia, Michael Schlömann
Salar de Uyuni, situated in the Southwest of the Bolivian Altiplano, is the largest salt flat on Earth. Brines of this athalassohaline hypersaline environment are rich in lithium and boron. Due to the ever- increasing commodity demand, the industrial exploitation of brines for metal recovery from the world's biggest lithium reservoir is likely to increase substantially in the near future. Studies on the composition of halophilic microbial communities in brines of the salar have not been published yet. Here we report for the first time on the prokaryotic diversity of four brine habitats across the salar...
June 2017: Microbiological Research
https://www.readbyqxmd.com/read/28454705/two-low-complexity-ultra-high-throughput-methods-to-identify-diverse-chemically-bioactive-molecules-using-saccharomyces-cerevisiae
#17
Katarina Petrovic, Martin Pfeifer, Christian N Parker, Sven Schuierer, John Tallarico, Dominic Hoepfner, N Rao Movva, Gunther Scheel, Stephen B Helliwell
The budding yeast S. cerevisiae is widely used as a eukaryotic model organism to elucidate the mechanism of action of low molecular weight compounds. This report describes the development of two high throughput screening methods based on cell viability either by monitoring the reduction of alamarBlue(®) (resazurin) or by direct optical measurement of cell growth. Both methods can be miniaturized to allow screening of large numbers of samples, and can be performed using S. cerevisiae in 384 and 1536-well format...
June 2017: Microbiological Research
https://www.readbyqxmd.com/read/28454704/the-normal-vaginal-and-uterine-bacterial-microbiome-in-giant-pandas-ailuropoda-melanoleuca
#18
Xin Yang, Guangyang Cheng, Caiwu Li, Jiang Yang, Jianan Li, Danyu Chen, Wencheng Zou, SenYan Jin, Hemin Zhang, Desheng Li, Yongguo He, Chengdong Wang, Min Wang, Hongning Wang
While the health effects of the colonization of the reproductive tracts of mammals by bacterial communities are widely known, there is a dearth of knowledge specifically in relation to giant panda microbiomes. In order to investigate the vaginal and uterine bacterial diversity of healthy giant pandas, we used high-throughput sequence analysis of portions of the 16S rRNA gene, based on samples taken from the vaginas (GPV group) and uteri (GPU group) of these animals. Results showed that the four most abundant phyla, which contained in excess of 98% of the total sequences, were Proteobacteria (59...
June 2017: Microbiological Research
https://www.readbyqxmd.com/read/28285663/an-lrr-receptor-kinase-regulates-growth-development-and-pathogenesis-in-phytophthora-capsici
#19
Asma Safdar, Qi Li, Danyu Shen, Linlin Chen, Feng He, Rongbo Wang, Meixiang Zhang, Joseph Juma Mafurah, Sajid Aleem Khan, Daolong Dou
Leucine-rich repeats (LRRs) domain containing kinase proteins (LRR-RK) perform various functions in eukaryotic organisms. However, their functions in Oomycetes are still largely unknown. Here, we identified an LRR-RK (PcLRR-RK1) gene and characterized its functions in Phytophthora capsici, a model oomycete specie and a major plant destroyer of solanaceous and cucurbitaceous vegetable crops. We showed that PcLRR-RK1-silenced P. capsici transformants exhibited reduced growth and produced highly branched fluffy hyphae...
May 2017: Microbiological Research
https://www.readbyqxmd.com/read/28285662/corrigendum-to-cellulolytic-potential-of-probiotic-bacillus-subtilis-ams6-isolated-from-traditional-fermented-soybean-churpi-an-in-vitro-study-with-regards-to-application-as-an-animal-feed-additive-microbiol-res-186-187-2016-62-70
#20
Ajay K Manhar, Yasir Bashir, Devabrata Saikia, Dhrubajyoti Nath, Kuldeep Gupta, Bolin K Konwar, Rahul Kumar, Nima D Namsa, Manabendra Mandal
No abstract text is available yet for this article.
May 2017: Microbiological Research
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