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Microbiological Research

Sravani Ankati, T Swaroopa Rani, Appa Rao Podile
Plant growth promoting rhizobacteria (PGPR) promote plant growth and activate defense response against phytopathogens. At the subcellular level plant-PGPR interaction is less understood, which would be essential for future improvement(s) of PGPR formulations. In a rigorous screening process, that also involved efficient PGPR strains, Bacillus sonorensis RS4 was selected to study partner-triggered interactions. The potential of B. sonorensis RS4 to improve growth of groundnut, efficiency to colonize roots, and influence on root topology was assessed...
December 2018: Microbiological Research
Yuichi Sakamoto, Shiho Sato, Miyuki Ito, Yuki Ando, Kiyoshi Nakahori, Hajime Muraguchi
Light and nutrients are crucial environmental factors influencing fungal sexual reproduction. Blue light induces simultaneous hyphal knot formation in Coprinopsis cinerea mycelia grown on low-glucose media but not in mycelia grown on high-glucose media. Many hyphal knots are visible in the arc near the edge of the colony one day after 15 min of blue light stimulation. These findings collectively suggest that blue light accelerates hyphal knot induction in nutrient-limited conditions. Transcriptome analysis revealed that gene expression after light exposure is divided into at least two major stages...
December 2018: Microbiological Research
Luisa F Posada, J C Álvarez, Magally Romero-Tabarez, Luz de-Bashan, Valeska Villegas-Escobar
Bacillus subtilis EA-CB0575 is a plant growth-promoting bacterium (PGPB) associated with banana and tomato crops. Root colonization is an important trait for PGPB microorganisms and potentiates the bacterial effect related to the mechanisms of plant growth promotion. Therefore, detection of bacterial colonization of roots in different culture systems is important in the study of plant-microorganism interactions. In this study, fluorescent in situ hybridization (FISH) and catalyzed reporter deposition-FISH (CARD-FISH) were evaluated to determine the colonization ability of B...
December 2018: Microbiological Research
Marika Rossi, Massimo Pesando, Marta Vallino, Luciana Galetto, Cristina Marzachì, Raffaella Balestrini
Many aspects of plant diseases caused by phytoplasmas are still unknown, as these pathogens are phloem restricted, uncultivable wall-less bacteria and must be studied always in association with their host. Phytoplasma transcripts are strongly underrepresented within host tissues and this poses problems for gene expression analyses. In this study, a procedure was established to infect the model plant Arabidopsis thaliana with the phytoplasma Flavescence dorée, a serious threat to European viticulture. Rates of phytoplasma infective insects and transmission efficiency to A...
December 2018: Microbiological Research
Miaochun Fan, Zhenshan Liu, Lijun Nan, Entao Wang, Weimin Chen, Yanbing Lin, Gehong Wei
Multiple heavy metals (HMs) commonly coexist in mining areas, which highlights the necessity to select multiple HM-resistant plant growth-promoting bacteria for improving phytoremediation efficiency. In this study, we isolated and characterized 82 endophytic bacteria from the root nodules of black locust (Robinia pseudoacacia) grown in a Pb-Zn mining area. There were 80 isolates showing resistance to four HMs, 0.01-18.0 mM/L for Cd, 0.2-40.0 mM/L for Zn, 0.3-2.2 mM/L for Pb, and 0.2-1.4 mM/L for Cu...
December 2018: Microbiological Research
Bamisope Steve Bamisile, Chandra Kanta Dash, Komivi Senyo Akutse, Ravindran Keppanan, Oluwatoyin Grace Afolabi, Mubasher Hussain, Muhammad Qasim, Liande Wang
Entomopathogenic fungi (EPF) can be established as endophytes in the host plants to offer a long-term preventive measure for pests and diseases. This practice serves as a better alternative to the common practice of periodic direct application of EPF on plants or the target pests as a short-term defense strategy against pests and diseases. These fungal endophytes, aside from their role in pests and diseases prevention, also act as plant growth promoters. Several fungal endophytes have been associated with improvement in plant height, dry and wet weight and other growth parameters...
December 2018: Microbiological Research
Sana Azaiez, Imen Ben Slimene, Ines Karkouch, Rym Essid, Selim Jallouli, Naceur Djebali, Salem Elkahoui, Ferid Limam, Olfa Tabbene
Four hundred and fifty bacteria were evaluated for antagonistic activity against bacterial soft rot of potato caused by Pectobacterium carotovorum sp strain II16. A strain Ar10 exhibiting potent antagonist activity has been identified as Bacillus amyloliquefaciens on the basis of biochemical and molecular characterization. Cell free supernatant showed a broad spectrum of antibacterial activity against human and phytopathogenic bacteria in the range of 10-60 AU/mL. Incubation of P. carotovorum cells with increasing concentrations of the antibacterial compound showed a killing rate of 94...
December 2018: Microbiological Research
María Ordóñez-Robles, Antonio Rodríguez-García, Juan F Martín
Chitin is the second most abundant carbohydrate biopolymer present in soils and is utilized by antibiotic-producing Streptomyces species. Its monomer, N-acetylglucosamine (GlcNAc), regulates the developmental program of the model organism Streptomyces coelicolor. GlcNAc blocks differentiation when growing on rich medium whilst it promotes development on poor culture media. However, it is unclear if the same GlcNAc regulatory profile observed in S. coelicolor applies also to other industrially important Streptomyces species...
December 2018: Microbiological Research
Bin Wang, Xuejie Li, Dou Yu, Xiaoyi Chen, Jioji Tabudravu, Hai Deng, Li Pan
Epigenetic modification is an important regulatory mechanism in the biosynthesis of secondary metabolites in Aspergillus species, which have been considered to be the treasure trove of new bioactive secondary metabolites. In this study, we reported that deletion of the epigenetic regulator gcnE, a histone acetyltransferase in the SAGA/ADA complex, resulted in the production of 12 polyketide secondary metabolites in A. niger FGSC A1279, which was previously not known to produce toxins or secondary metabolites...
December 2018: Microbiological Research
Xinqing Huang, Jianming Zhu, Zhonghua Cai, Yongmin Lao, Hui Jin, Ke Yu, Boya Zhang, Jin Zhou
The complicated relationships among environmental microorganisms are regulated by quorum sensing (QS). Understanding QS-based signals could shed light on the interactions between microbial communities in certain environments. Although QS characteristics have been widely discussed, few studies have been conducted on the role of QS in phycospheric microorganisms. Here, we used metagenomics to examine the profile of AI-1 (AinS, HdtS, LuxI) and AI-2 (LuxS) autoinducers from a deeply sequenced microbial database, obtained from a complete dinoflagellate bloom...
December 2018: Microbiological Research
Aehtesham Hussain, Muzafar Ahmad Rather, Mohd Saleem Dar, N A Dangroo, Mushtaq A Aga, Arem Qayum, Aabid Manzoor Shah, Zahoor Ahmad, Mohd Jamal Dar, Qazi Parvaiz Hassan
No abstract text is available yet for this article.
December 2018: Microbiological Research
Yang Guanhua, Cheng Wang, Xuetong Wang, Ruiqing Ma, Huajun Zheng, Qin Liu, Yuanxing Zhang, Yue Ma, Qiyao Wang
Vibrio anguillarum is a notorious bacterial pathogen that causes vibriosis in various marine farmed fish species. The highly pathogenic V. anguillarum strain MVM425 has been isolated from moribund turbot in the China Yellow Sea, but the genetic basis for its pathogenesis is undefined. Complete genome sequencing uncovered that the bacterium encodes 3985 protein-coding sequences (CDSs) on two chromosomes and 62 CDSs on the virulence plasmid pEIB1. To investigate the genes that are essential for its in vitro and in vivo growth, we applied transposon insertion sequencing technology (Tn-seq) to screen a highly saturated transposon insertion mutant library grown in Luria-Bertani broth with 2% NaCl (LB20) and in its natural host turbot...
November 2018: Microbiological Research
Andrea Santana de Oliveira, Isabella Inês Rodrigues Rosa, Evandro Novaes, Lucas Silva de Oliveira, Lilian Cristiane Baeza, Clayton Luiz Borges, Lennart Marlinghaus, Célia Maria de Almeida Soares, Marcia Giambiagi-deMarval, Juliana Alves Parente-Rocha
Staphylococcus saprophyticus is a gram-positive microorganism responsible for urinary tract infections (UTIs). Although some virulence factors are characterized, such as urease, autolysins, adhesins and hemagglutinins, large-scale proteomic studies have not been performed within this species. We performed the characterization of the exoproteome from three S. saprophyticus strains: the reference strain ATCC 15,305, a non-capsular strain 7108 and the 9325 strain containing a thick capsule which were cultured in BHI medium and culture supernatants were analysed by using mass spectrometry approach...
November 2018: Microbiological Research
Semcheddine Cherrad, Aline Charnay, Catalina Hernandez, Herve Steva, Lassaad Belbahri, Sébastien Vacher
The grapevine powdery mildew Erysiphe necator (E. necator) is an obligate pathogen. Powdery mildew-diseased vines show an important reduction in plant size, winter hardiness and grape yield. Even a low-level infection with powdery mildew was shown to taint wine and ultimately reduce wine quality. For many years, succinate dehydrogenase inhibitor (SDHI) fungicides, mainly the new generation active ingredients (AIs) boscalid, penthiopyrad and fluopyram, have been widely used to control powdery mildew in grapevines...
November 2018: Microbiological Research
Dan Peng, Xuan Li, Pin Liu, Xipeng Zhou, Mei Luo, Kewen Su, Shuai Chen, Zhongshuang Zhang, Qiang He, Jingfu Qiu, Yingli Li
RcsAB is an atypical two-component regulatory system that can regulate exopolysaccharide biosynthesis and is involved in the virulence of K. pneumoniae. The gene galF is well known as a gene involved in the biosynthesis of capsular polysaccharide (CPS). The specific DNA identification sequence for transcriptional regulation of RcsAB was found to be present in the promoter region of galF. This study aimed to detect the function of RcsAB in virulence and in biofilm and CPS formation. In addition, the transcriptional regulation of the galF gene in K...
November 2018: Microbiological Research
Afshan Majeed, M Kaleem Abbasi, Sohail Hameed, Sumera Yasmin, Muhammad Kashif Hanif, Tahir Naqqash, Asma Imran
Plant growth promoting rhizobacteria (PGPR) are capable to increase the growth and yield of crops in eco-friendly and sustainable manner. To evaluate the response of sunflower towards inoculation with PGPR, a sunflower root associated bacterium AF-54 isolated from Diyar Gali Himalayan Mountain region, Azad Jammu and Kashmir (AJK), identified as Pseudomonas sp. by 16S rRNA sequence analysis and was characterized using polyphasic approach. The bacterium produced 23.9 μgmL-1 indole-3-acetic acid in tryptophan-supplemented medium, showed 44...
November 2018: Microbiological Research
Abeer F Elhagrassy
A total of 35 actinomycetes were isolated from the surface of mural paintings of snu- sort- ankh in El Leasht, Egypt during four seasons all over 2016-2017. In 2009 this tomb was deteriorated by "Aspergillus niger, A. flavus, Fusarium moniliforme, Alternaria alternate, Rhizopus stolonifera, Bacillus subtilis, Bacillus cereus, and micrococcus iuteus." In 2017 the isolation of swabs presents only Aspergillus niger and about 35 actinomycetes classified to three different groups "Streptomyces, Nocardia, and Micromonospora" only five species belong to Streptomyces group showed antimicrobial activity against the previous microorganisms...
November 2018: Microbiological Research
Chinyere A Knight, Michael J Bowman, Lafayette Frederick, Agnes Day, Clarence Lee, Christopher A Dunlap
A strain of bacteria in the Bacillus subtilis species complex was isolated from a building's air vent in the Washington DC area, USA, and produced strong antifungal activity with in vitro assays. This strain, designated (HU Biol-II), showed pronounced inhibitory effects on mycelial growth of a wide spectrum of fungi. The objectives of this study were to use genome sequencing to confirm the taxonomy of HU Biol-II, evaluate its antifungal activity and implement genome mining and HPLC-MS/MS to characterize the bioactive secondary metabolites...
November 2018: Microbiological Research
Huei-Ru Lin, Hung-Yu Shu, Guang-Huey Lin
Indole-3-acetic acid (IAA) is an important plant hormone, and many types of bacteria interact with plants by producing or degrading IAA in the rhizosphere. The iac (indole-3-acetic acid catabolism) gene locus in Acinetobacter baumannii ATCC19606 was previously associated with IAA degradative capability, and in this study, transcriptome analysis results derived from A. baumannii cultured with IAA showed that the expression of catechol-degrading and phenylacetate-degrading genes was elevated, indicating that IAA is likely degraded through these pathways...
November 2018: Microbiological Research
Zhiping Han, Liisa Kautto, Wieland Meyer, Sharon C-A Chen, Helena Nevalainen
One of the micro-environmental stresses that fungal pathogens, such as Scedosporium aurantiacum, colonising human lungs encounter in vivo is hypoxia, or deficiency of oxygen. In this work, we studied the impacts of a hypoxic micro-environment (oxygen levels ≤1%) on the growth of a clinical S. aurantiacum isolate (WM 06.482; CBS 136046) and an environmental strain (S. aurantiacum WM 10.136; CBS 136049) on mucin-containing synthetic cystic fibrosis sputum medium. Additionally, profiles of secreted proteases were compared between the two isolates and protease activity was assessed using class-specific substrates and inhibitors...
November 2018: Microbiological Research
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