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Acta Microbiologica et Immunologica Hungarica

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https://www.readbyqxmd.com/read/27848253/role-of-health-care-professionals-in-preventative-activities-and-vaccination-programs-during-outbreaks
#1
Sándor Balogh, Renáta Papp, Csilla Busa, Ágnes Csikós
PURPOSE: The general purpose of TELL ME study was to give an insight into the experiences of European family physicians with management of H1N1 pandemic flu. METHODS: Qualitative research methods (focus group discussions, one-to-one interviews, and online data collection) were used to explore family doctors' opinion and suggestions. Overall 158 family physicians took part in the study from six European countries. RESULTS: Family doctors' most important experience was that the official campaign was not able to compensate negative effects of the mass media...
November 16, 2016: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/27842452/role-of-bola-and-rpos-genes-in-biofilm-formation-and-adherence-pattern-by-escherichia-coli-k-12-mg1655-on-polypropylene-stainless-steel-and-silicone-surfaces
#2
Mohd Adnan, Ana Margarida Sousa, Idalina Machado, Maria Olivia Pereira, Saif Khan, Glyn Morton, Sibte Hadi
Escherichia coli has developed sophisticated means to sense, respond, and adapt in stressed environment. It has served as a model organism for studies in molecular genetics and physiology since the 1960s. Stress response genes are induced whenever a cell needs to adapt and survive under unfavorable growth conditions. Two of the possible important genes are rpoS and bolA. The rpoS gene has been known as the alternative sigma (σ) factor, which controls the expression of a large number of genes, which are involved in responses to various stress factors as well as transition to stationary phase from exponential form of growth...
November 15, 2016: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/27842441/identification-of-two-substrates-of-fts_1067-protein-an-essential-virulence-factor-of-francisella-tularensis
#3
Petra Spidlova, Iva Senitkova, Marek Link, Jiri Stulik
Francisella tularensis is a highly virulent intracellular pathogen with the capacity to infect a variety of hosts including humans. One of the most important proteins involved in F. tularensis virulence and pathogenesis is the protein DsbA. This protein is annotated as a lipoprotein with disulfide oxidoreductase/isomerase activity. Therefore, its interactions with different substrates, including probable virulence factors, to assist in their proper folding are anticipated. We aimed to use the immunopurification approach to find DsbA (gene locus FTS_1067) interacting partners in F...
November 15, 2016: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/27842453/aflatoxigenic-aspergillus-flavus-and-aspergillus-parasiticus-strains-in-hungarian-maize-fields
#4
Flóra Sebők, Csaba Dobolyi, Dóra Zágoni, Anita Risa, Csilla Krifaton, Mátyás Hartman, Mátyás Cserháti, Sándor Szoboszlay, Balázs Kriszt
Due to the climate change, aflatoxigenic Aspergillus species and strains have appeared in several European countries, contaminating different agricultural commodities with aflatoxin. Our aim was to screen the presence of aflatoxigenic fungi in maize fields throughout the seven geographic regions of Hungary. Fungi belonging to Aspergillus section Flavi were isolated in the ratio of 26.9% and 42.3% from soil and maize samples in 2013, and these ratios decreased to 16.1% and 34.7% in 2014. Based on morphological characteristics and the sequence analysis of the partial calmodulin gene, all isolates proved to be Aspergillus flavus, except four strains, which were identified as Aspergillus parasiticus...
November 14, 2016: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/27682850/book-reviewreviews
#5
Orsolya Nagy, Mária Takács
Review of the book "RNA/DNA and Cancer" written by Joseph G. Sinkovics (Springer International Publishing, Switzerland, 2016, ISBN 978-3-319-22278-3, DOI 10.1007/978-3-319-22279-0).
September 2016: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/27682849/banana-fruits-affected-by-fusarium-post-harvest-disease-as-source-of-human-fusariosis
#6
David Triest
No abstract text is available yet for this article.
September 2016: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/27682848/dr3-regulation-of-apoptosis-of-naive-t-lymphocytes-in-children-with-acute-infectious-mononucleosis
#7
Elena Nikolaevna Filatova, Elena Viktorovna Anisenkova, Nataliya Borisovna Presnyakova, Oleg Vladimirovich Utkin
Acute infectious mononucleosis (AIM) is a widespread viral disease that mostly affects children. Development of AIM is accompanied by a change in the ratio of immune cells. This is provided by means of different biological processes including the regulation of apoptosis of naive T-cells. One of the potential regulators of apoptosis of T-lymphocytes is a death receptor 3 (DR3). We have studied the role of DR3 in the regulation of apoptosis of naive CD4(+) (nTh) and CD8(+) (nCTL) T-cells in healthy children and children with AIM...
September 2016: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/27682847/quantification-of-biofilm-formation-on-silicone-intranasal-splints-an-in-vitro-study
#8
Bojan Pavlović, Dragana D Božić, Jovica Milovanović, Ana Jotić, Vojko Djukić, Slobodanka Djukić, Neda Konstantinović, Ivana Ćirković
OBJECTIVES: Biofilms are associated with persistent infections and resistant to conventional therapeutic strategies. The aim of this study was to investigate the quantity of biofilm produced on silicone intranasal splints. METHODS: Quantity of biofilm formation on silicone splints (SS) was tested on 15 strains of Staphylococcus aureus and Moraxella catarrhalis, respectively. Antimicrobial susceptibility testing was performed in accordance with European Committee on Antimicrobial Susceptibility Testing recommendations...
September 2016: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/27576919/laboratory-diagnosis-of-acanthamoeba-keratitis-in-hungary
#9
Erika Orosz, Ágnes Farkas, István Kucsera
Acanthamoeba species are free-living amebae that can be found in almost every range of environments. Within this genus, numerous species are recognized as human pathogens, potentially causing Acanthamoeba keratitis (AK). AK is a corneal disease that is predominantly associated with contact lens use, the epidemiology of which is related to the specific genotype of Acanthamoeba. This study reports seven (7/16; 43.75%) positive cases. Detection of Acanthamoeba in corneal scrapings is based on cultivation and polymerase chain reaction (PCR) combined with the molecular taxonomic identification method...
September 2016: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/27539331/plasmid-mediated-quinolone-resistance-among-extended-spectrum-beta-lactamase-producing-enterobacteriaceae-from-bloodstream-infections
#10
Judit Domokos, Katalin Kristóf, Dóra Szabó
The purpose of this study was to determine prevalence and molecular characterization of plasmid-mediated quinolone resistance (PMQR) genes [qnrA, qnrB, qnrC, qnrD, qnrS, aac(6')-Ib-cr, qepA, and oqxAB] among extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella spp. isolates from bloodcultures in Hungary. A total of 103 isolates were tested for quinolone susceptibility by microdilution method and PMQR genes were detected by polymerase chain reaction. About 40 ESBL-producing E. coli (39%) and 50 ESBL-producing Klebsiella spp...
August 19, 2016: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/27539330/immunomodulatory-effects-of-anti-microbial-peptides
#11
Laszlo Otvos
Anti-microbial peptides (AMPs) were originally thought to exert protecting actions against bacterial infection by disintegrating bacterial membranes. Upon identification of internal bacterial targets, the view changed and moved toward inhibition of prokaryote-specific biochemical processes. However, the level of none of these activities can explain the robust efficacy of some of these peptides in animal models of systemic and cutaneous infections. A rapidly growing panel of reports suggests that AMPs, now called host-defense peptides (HDPs), act through activating the immune system of the host...
August 19, 2016: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/27539329/lectins-and-tetrahymena-a-review
#12
György Csaba
The unicellular ciliate Tetrahymena is a complete organism, one of the most highly developed protozoans, which has specialized organelles performing each of the functions characteristic to the cells of higher ranked animals. It is also able to produce, store, and secrete hormones of higher ranked animals and also react to them. It produces lectins that can bind them and has functions, which are influenced by exogenous lectins. The review lists the observations on the relationship between lectins and Tetrahymena and try to construe them on the basis of the data, which are at our disposal...
August 19, 2016: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/27539229/ebola-virus-disease-awareness-among-dental-students-in-hungary
#13
Katalin Károlyházy, Balázs Fazekas, Janka Fazekas, Péter Hermann, Krisztina Márton
INTRODUCTION: The Ebola epidemic has claimed thousands of lives in Africa, and there has been a mounting pressure on the healthcare systems around the globe to prepare for the showing up of patients infected with this virus. Junior clinicians are at the forefront of medical teams, often coming into contact with patients first, during clerking and admissions. This study assesses the level of knowledge of Ebola virus disease (EVD) among dental students at Semmelweis University, Budapest, Hungary...
August 19, 2016: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/27352976/equine-like-g3-rotavirus-in-hungary-2015-is-it-a-novel-intergenogroup-reassortant-pandemic-strain
#14
Renáta Dóró, Szilvia Marton, Anett Horváth Bartókné, György Lengyel, Zsófia Agócs, Ferenc Jakab, Krisztián Bányai
Novel, intergenogroup reassortant G3 rotavirus strains are spreading in at least three continents: Asia, Australia, and Europe. The present study provides evidence that a closely related G3P[8] strain circulated in Hungary during 2015. Whole genome sequencing and phylogenetic analysis showed that the identified strain continues to evolve by reassortment. This observation demonstrates the genomic plasticity of the novel strain, which is thought to be a prerequisite of the success of emerging rotavirus genotypes...
June 2016: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/27352975/microbiological-investigations-on-the-water-of-a-thermal-bath-at-budapest
#15
Sára Szuróczki, Zsuzsa Kéki, Szandra Káli, Anett Lippai, Károly Márialigeti, Erika Tóth
Thermal baths are unique aquatic environments combining a wide variety of natural and anthropogenic ecological factors, which also appear in their microbiological state. There is limited information on the microbiology of thermal baths in their complexity, tracking community shifts from the thermal wells to the pools. In the present study, the natural microbial community of well and pool waters in Gellért bath was studied in detail by cultivation-based techniques. To isolate bacteria, 10% R2A and minimal synthetic media (with "bath water") with agar-agar and gellan gum were used after prolonged incubation time; moreover, polyurethane blocks covered with media were also applied...
June 2016: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/27352974/education-of-hand-rubbing-technique-to-prospective-medical-staff-employing-uv-based-digital-imaging-technology
#16
Ákos Lehotsky, László Szilágyi, Annamária Demeter-Iclănzan, Tamás Haidegger, György Wéber
The aim of this study was to objectively assess the hand hygiene performance of medical students. Hand rubbing technique was evaluated by employing innovative UV-light-based imaging technology, identifying patterns and trends in missed areas after applying WHO's six-step protocol. This specially designed hand hygiene education and assessment program targeted 1,344 medical students at two distant sites in Central Europe. Students were introduced to a short video, presenting the basics of hand hygiene, and then received further demonstration from professional trainers, focusing on the correct execution of WHO's six-step technique...
June 2016: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/27352973/salmonella-infection-in-healthy-pet-reptiles-bacteriological-isolation-and-study-of-some-pathogenic-characters
#17
Fabrizio Bertelloni, Marianne Chemaly, Domenico Cerri, Françoise Le Gall, Valentina Virginia Ebani
The fecal samples from 213 captive reptiles were examined, and 29 (13.61%) Salmonella enterica isolates were detected: 14/62 (22.58%) from chelonians, 14/135 (10.37%) from saurians, and 1/16 (6.25%) from ophidians. The isolates were distributed among 14 different serotypes: Miami, Ebrie, Hermannsweder, Tiergarten, Tornov, Pomona, Poona, Goteborg, Abaetetube, Nyanza, Kumasi, Typhimurium, 50:b:z6, 9,12:z29:1,5, and a non-motile serotype with antigenic formula 1,4,[5],12:-:-. Salmonella typhimurium and 50:b:z6 isolates showed the spv plasmid virulence genes, responsible of the capability to induce extra-intestinal infections...
June 2016: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/27352972/a-rough-set-based-rational-clustering-framework-for-determining-correlated-genes
#18
Jeba Emilyn Jeyaswamidoss, Kesavan Thangaraj, Kadarkarai Ramar, Muthusamy Chitra
Cluster analysis plays a foremost role in identifying groups of genes that show similar behavior under a set of experimental conditions. Several clustering algorithms have been proposed for identifying gene behaviors and to understand their significance. The principal aim of this work is to develop an intelligent rough clustering technique, which will efficiently remove the irrelevant dimensions in a high-dimensional space and obtain appropriate meaningful clusters. This paper proposes a novel biclustering technique that is based on rough set theory...
June 2016: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/27352971/laboratory-identification-of-anaerobic-bacteria-isolated-on-clostridium-difficile-selective-medium
#19
Cristina Rodriguez, Nathalie Warszawski, Nicolas Korsak, Bernard Taminiau, Johan Van Broeck, Michel Delmée, Georges Daube
Despite increasing interest in the bacterium, the methodology for Clostridium difficile recovery has not yet been standardized. Cycloserine-cefoxitin fructose taurocholate (CCFT) has historically been the most used medium for C. difficile isolation from human, animal, environmental, and food samples, and presumptive identification is usually based on colony morphologies. However, CCFT is not totally selective. This study describes the recovery of 24 bacteria species belonging to 10 different genera other than C...
June 2016: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/27352970/effect-of-topical-anti-streptococcus-mutans-igy-gel-on-quantity-of-s-mutans-on-rats-tooth-surface
#20
Endang W Bachtiar, Anggraeni Afdhal, Ratna Meidyawati, Retno D Soejoedono, Erni Poerwaningsih
This study aims to evaluate the effect of anti-Streptococcus mutans IgY gel on quantity of S. mutans on rats' tooth surface. Sprague Dawley rats were exposed intra-orally with S. mutans Xc and were fed a caries-inducing diet 2000. The 24 rats were divided into four groups: group A had their teeth coated with IgY gel; group B received sterilized water as a control; group C had their teeth coated with IgY gel starting on the 29(th) day; and group D had their teeth coated with a gel without IgY. Plaque samples were swabbed from the anterior teeth for S...
June 2016: Acta Microbiologica et Immunologica Hungarica
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