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Journal of Microbiology and Biotechnology

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https://www.readbyqxmd.com/read/28081360/pinus-densiflora-bark-extract-pinexol-decreased-adiposity-in-mice-by-down-regulation-of-hepatic-de-novo-lipogenesis-and-adipogenesis-in-white-adipose-tissue
#1
Hyemyoung Ahn, Gwang-Woong Go
PineXol, extracted from Korean red pine bark, has beneficial effects, such as antioxidant, anti3 inflammatory, and anti-lipogenic activities in vitro. We tested the hypothesis that PineXol supplementation could have anti-obesity effects on mice fed a high-fat diet (HFD). Four-week old male C57BL/6 mice were fed normal chow (18% kcal from fat) or HFD (60% kcal from fat). HFD-fed animals were also subjected to PineXol treatment at a dose of 10 or 50 mg/kg body weight (BW) (PX10 or PX50, respectively) body weight...
January 12, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28081359/benzoic-acid-enhances-embryo-implantation-through-lif-dependent-expression-of-integrin-%C3%AE-v%C3%AE-3-and-%C3%AE-v%C3%AE-5
#2
Hee-Jung Choi, Tae-Wook Chung, Mi-Ju Park, Hyung Sik Kim, Sooseong You, Myeong Soo Lee, Bo Sun Joo, Kyu Sup Lee, Keuk-Jun Kim, Gabbine Wee, Choong-Yong Kim, Cheorl-Ho Kim, Ki-Tae Ha
The embryo implantation is crucial step for successful pregnancy. Diverse factors including adhesion molecules, growth factors, and cytokines are important to the embryo implantation through improving endometrial receptivity. Benzoic acid (BA), a component of various plants, has been shown to have anti-fungal and anti-oxidant effects. However, the effect of BA on the embryo implantation remains unknown. Here, we showed the contribution of BA for the enhancement of endometrial receptivity through the leukemia inhibitory factor (LIF)-dependent increase of integrin αV, β3, and β5 expression...
January 12, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28081358/microbial-diversity-during-fermentation-of-sweet-paste-a-chinese-traditional-seasoning-using-pcr-denaturing-gradient-gel-electrophoresis
#3
Ping Mao, Yuanliang Hu, Tingting Liao, Zhaoting Wang, Shumiao Zhao, Yunxiang Liang, Yongmei Hu
The aim of this study was to elucidate the changes in the microbial community and biochemical properties of a traditional sweet paste during fermentation. PCR-denaturing gradient gel electrophoresis (DGGE) analysis showed that Aspergillus oryzae was the predominant species in the koji and the majority of the fungi isolated belonged to 2 Zygosaccharomyces species in the mash. The bacterial DGGE profiles revealed the presence of Bacillus subtilis during fermentation, and Lactobacillus acidipiscis, Lactobacillus pubuzihii, Lactobacillus sp, Staphylococcus kloosi and several uncultured bacteria were also detected in the mash after 14 days of main fermentation...
January 12, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28081357/assessment-of-neuronal-cell-based-cytotoxicity-of-neurotoxins-from-an-estuarine-nemertean-in-the-han-river-estuary
#4
Yeo Seon Kwon, Seul Ki Min, Seung Ju Yeon, Jin Ha Hwang, Jae-Sang Hong, Hwa Sung Shin
A heteronemertean, Yininemertes pratensis was collected in Han River Estuary, South Korea. This estuarine nemertean has been known for harmful effects by the local fishermen to the glass eels, juveniles of Japanese eels Anguilla japonica, migrating to fresh water. The present study confirmed the neurotoxic effects of this heteronemertean ribbon worm at the cellular level. 5, 11-dideoxy TTX (288 m/z) and 11-norTTX-6(S)-01 (m/z 305.97), derivative types of neurotoxic TTX, were identified through HPLC and MALDI-TOF MS...
January 12, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28081356/canna-edulis-leaf-extract-mediated-preparation-of-stabilized-silver-nanoparticles-characterization-antimicrobial-activity-and-toxicity-studies
#5
S V Otari, S H Pawar, Sanjay K S Patel, Raushan K Singh, Sang-Yong Kim, Jai Hyo Lee, Liaoyuan Zhang, Jung-Kul Lee
A novel approach to synthesize silver nanoparticles (AgNPs) using leaf extract of Canna edulis Ker-Gawl. (CELE) under ambient conditions is reported here. The as-prepared AgNPs were analyzed by UV-visible spectroscopy, transmission emission microscopy, X-ray diffraction, Fourier transform-infra red spectroscopy, energy-dispersive analysis of X-ray spectroscopy, zeta potential, and dynamic light scattering. The AgNPs showed excellent antimicrobial activity against various pathogens, including bacteria and various fungi...
January 12, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28081355/oral-delivery-of-probiotics-in-poultry-using-ph-sensitive-tablets
#6
Tao Jiang, Hui-Shan Li, Geon Goo Han, Bijay Singh, Sang-Kee Kang, Jin-Duck Bok, Dae-Duk Kim, Zhong-Shan Hong, Yun-Jaie Choi, Chong-Su Cho
As alternatives to antibiotics in live stocks, probiotics have been used although most of them in the form of liquid or semisolid formulations which show low cell viability after oral administration. Therefore, suitable dry dosage forms should be developed for live stocks to protect probiotics against the low pH in the stomach such that the products have higher probiotics survivability. Here, in order to develop a dry dosage forms of probiotics for poultry, we used hydroxyproyl methylcellulose phthalate 55 (HPMCP 55) as a tablet forming matrix to develop probiotics in a tablet form for poultry...
January 12, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28068666/mitigation-of-membrane-biofouling-in-mbr-using-a-cellulolytic-bacterium-undibacterium-sp-dm-1-isolated-from-activated-sludge
#7
Chang Hyun Nahm, Seonki Lee, Sang Hyun Lee, Kibaek Lee, Jaewoo Lee, Hyeokpil Kwon, Kwang-Ho Choo, Jung-Kee Lee, Jae Young Jang, Chung-Hak Lee, Pyung-Kyu Park
Biofilm formation on membrane surface results in the loss of permeability in membrane bioreactors (MBRs) for wastewater treatment. Studies have revealed that cellulose is not only produced by a number of bacterial species but also plays a key role during formation of their biofilm. Hence, in this study, cellulase was introduced to MBR as a cellulose-induced biofilm control strategy. For practical application of cellulase to MBR, a cellulolytic (i.e., cellulase-producing) bacterium, Undibacterium sp. DM-1, was isolated from a lab-scale MBR for wastewater treatment...
January 9, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28068665/potent-inhibition-of-monoamine-oxidase-b-by-a-piloquinone-from-a-marine-derived-streptomyces-sp-cnq-027
#8
Hyun Woo Lee, Hansol Choi, Sang-Jip Nam, William Fenical, Hoon Kim
Two piloquinone derivatives isolated from Streptomyces sp. CNQ-027 were tested for the inhibitory activities of two isoforms of monoamine oxidase (MAO), which catalyzes monoamine neurotransmitters. The piloquinone 4,7-dihydroxy-3-methyl-2-(4-methyl-1-oxopentyl)-6H-dibenzo[b,d]pyran-6-one (1) was found to be a highly potent inhibitor of human MAO-B, with an IC₅₀ value of 1.21 µM; in addition, it was found to be highly effective against MAO-A, with an IC₅₀ value of 6.47 µM. Compound 1 was selective, but not extremely so, for MAO-B compared to MAO-A, with a selectivity index value of 5...
January 9, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28068664/fusion-peptide-improves-stability-and-bioactivity-of-single-chain-antibody-against-rabies-virus
#9
Hualong Xi, Kaixin Zhang, Yanchun Yin, Tiejun Gu, Qing Sun, Linqing Shi, Renxia Zhang, Chunlai Jiang, Wei Kong, Yongge Wu
The combination of rabies immunoglobulin (RIG) with a vaccine is currently effective against rabies infections, but improvements are needed. Genetic engineering antibody technology is an attractive approach for developing novel antibodies to replace RIG. In our previous study, a single-chain variable fragment, scFv57R, against rabies virus glycoprotein (RVGP) was constructed. However, its inherent weak stability and short half-life compared with the parent RIG may limit its diagnostic and therapeutic application...
January 9, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28035121/combined-treatment-with-low-concentrations-of-aqueous-and-gaseous-chlorine-dioxide-inactivates-escherichia-coli-o157-h7-and-salmonella-typhimurium-inoculated-on-paprika
#10
Hyun-Gyu Kim, Kyung Bin Song
Combined treatment with gaseous and aqueous chlorine dioxide (ClO₂) was performed to improve the microbiological safety and quality of paprika. A single treatment of 50 ppmv ClO₂ gas for 30 min decreased the populations of Escherichia coli O157:H7 and Salmonella Typhimurium by 2.33 and 2.91 log CFU/g, respectively. In addition, a single treatment of aqueous ClO₂ (50 ppm) for 5 min decreased these populations by 1.86 and 1.37, respectively. The most dramatic effects were achieved by combined treatment of 50 ppm aqueous and gaseous ClO₂ for 30 min, which decreased populations of E...
December 30, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28035120/heme-derived-from-corynebacterium-glutamicum-a-potential-iron-additive-for-swine-and-an-electron-carrier-additive-for-lactic-acid-bacterial-culture
#11
Su-In Choi, Jihoon Park, Pil Kim
To investigate the potential applications of bacterial heme, ALA synthase (HemA) was expressed in a Corynebacterium glutamicum HA strain that had been adaptively evolved against oxidative stress. The red pigment from the constructed strain was extracted and it exhibited the typical heme absorbance at 408 nm from the spectrum. To investigate the potential of this strain as an iron additive for swine, a prototype feed additive was manufactured in pilot scale by culturing the strain in a 5-ton fermenter followed by spray-drying the biomass with flour as an excipient (biomass: flour = 1:10 w/w)...
December 30, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27994215/antimicrobial-activity-of-basil-oregano-and-thyme-essential-oils
#12
REVIEW
Hercules Sakkas, Chrissanthy Papadopoulou
For centuries plants have been used for a wide variety of purposes, from treating infectious diseases to food preservation and perfume production. Presently, the increasing resistance of microorganisms to currently used antimicrobials in combination with the appearance of emerging diseases requires urgently the development of new, more effective drugs. Plants, due to the large biological and structural diversity of their components, constitute a unique and renewable source for the discovery of new antibacterial, antifungal and antiparasitic treatments...
December 20, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27994214/microbial-%C3%AE-galactosidase-of-pediococcus-pentosaceus-id-7-isolation-cloning-and-molecular-characterization
#13
Ji-Yeong Lee, Mi-Sun Kwak, Jong-Bok Roh, Kwang Kim, Moon-Hee Sung
Pediococcus pentosaceus ID-7 was isolated from Kimchi, a Korean fermented food, and it showed high activity for lactose hydrolysis. The β-galactosidase of P. pentosaceus ID-7 belongs to the GH2 group, which is composed of two distinct proteins. The heterodimeric LacLM type of β-galactosidase found in P. pentosaceus ID-7 consists of two genes partially overlapped, lacL and lacM encoding the LacL (72.2 kDa) and LacM (35.4 kDa). In this study, Escherichia coli MM294 was used for the production of LacL, LacM, and LacLM...
December 20, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27994213/host-cell-nuclear-localization-of-shigella-flexneri-effector-ospf-is-facilitated-by-sumoylation
#14
Kyungmin Jo, Eun Jin Kim, Hyun Jin Yu, Cheol-Heui Yun, Dong Wook Kim
When Shigella infect host cells, various effecter molecules are delivered into cytoplasm of the host cell through the type III secretion system (TTSS) to facilitate their invasion process and control the host immune responses. Among these effectors, the S. flexneri effector OspF dephosphorylates mitogen-activated protein kinases (MAPK) and translocates itself to the nucleus, thus preventing histone H3 modification to regulate expression of pro-inflammatory cytokines. Despite the critical role of OspF, the mechanism by which OspF localizes in the nucleus has remained to be elucidated...
December 20, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27994212/intracellular-trafficking-modulation-by-ginsenoside-rg3-inhibits-brucella-abortus-uptake-and-intracellular-survival-within-raw-264-7-cells
#15
Tran Xuan Ngoc Huy, Alisha Wehdnesday Bernardo Reyes, Huynh Tan Hop, Lauren Togonon Arayan, WonGi Min, Hu Jang Lee, Man Hee Rhee, Hong Hee Chang, Suk Kim
Ginsenoside Rg3, a saponin extracted from Ginseng, has various pharmacological and biological activities, however its effects against Brucella infection are still unclear. Herein, the inhibitory effects of ginsenoside Rg3 against intracellular parasitic Brucella infection were evaluated through bacterial infection, adherence assays, and LAMP-1 colocalization, as well as immunoblotting and FACS for detecting MAPKs signaling proteins and F-actin polymerization, respectively. The internalization, intracellular growth and adherence of Brucella (B...
December 20, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27994211/proteome-changes-in-penicillium-expansum-grown-in-a-medium-derived-from-host-plant
#16
Xiaoshuang Xia, Huan Li, Fei Liu, Ye Zhang, Qi Zhang, Yun Wang, Peiwu Li
Penicillium expansum causes blue mould rot, a prevalent post-harvest disease of pome fruit, and is also the main producer of the patulin. However, knowledge on the molecular mechanisms involved in this pathogen-host interaction remains largely unknown. In this work, a two-dimensional gel electrophoresis (2-DE)-based proteomic approach was applied to probe changes in P. expansum cultivated in apple juice medium, which was used to mimic the in planta condition. The results showed that pH value and reducing sugar content in the apple juice medium decreased whereas the patulin content increased with the growing of P...
December 20, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27974738/significance-of-viable-but-nonculturable-escherichia-coli-induction-detection-and-control
#17
REVIEW
Tian Ding, Yuanjie Suo, Qisen Xiang, Xihong Zhao, Shiguo Chen, Xingqian Ye, Donghong Liu
Diseases caused by foodborne or waterborne pathogens are emerging. Many pathogens can enter into the viable but nonculturable (VBNC) state, which is a survival strategy when exposed to harsh environmental stresses. Pathogens in the VBNC state have the ability to evade conventional microbiological detection methods that pose a significant and potential health risk. Therefore, controlling the VBNC bacteria in food processing and environment is of great importance. As the typical one of the Gram-negatives, Escherichia coli (E...
December 14, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27974737/genomic-insights-and-its-comparative-analysis-with-yersinia-enterocolitica-reveals-the-potential-virulence-determinants-and-further-pathogenicity-for-food-borne-outbreaks
#18
Gopalsamy Gnanasekaran, Eun Jung Na, Han Young Chung, Suyeon Kim, You-Tae Kim, Woori Kwak, Heebal Kim, Sangryeol Ryu, Sang Ho Choi, Ju-Hoon Lee
Yersinia enterocolitica is a well-known food-borne pathogen causing gastrointestinal infections worldwide. The strain Y. enterocolitica FORC_002, isolated from the gill of flatfish (plaice) and its genome was sequenced. The genomic DNA consists of 4,837,317 bp with a GC content of 47.1%, and is predicted to contain 4,221 open reading frames (ORFs), 81 tRNA genes, and 26 rRNA genes. Interestingly, genomic analysis revealed pathogenesis and host immune evasion-associated genes encoding guanylate cyclase (Yst), invasin (Ail and Inv), outer membrane protein (Yops), autotransporter adhesin A (YadA), RTX-like toxins, and a type III secretion system (TTSS)...
December 14, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27974736/hed1-promotes-meiotic-crossover-formation-in-saccharomyces-cerevisiae
#19
Yoon-Ju Kong, Jeong-Hwan Joo, Keun Pil Kim, Soogil Hong
Homologous recombination (HR) occurs between homologous chromosomes and is significantly involved in programmed double-strand breaks (DSB) repair. Activation of two recombinases, Rad51 and Dmc1, is essential for an inter-homolog bias during meiosis. Rad51 participates in both mitotic and meiotic recombination, and its strand exchange activity is regulated by an inhibitory factor during meiosis. Thus, activities of Rad51 and Dmc1 are coordinated to promote homolog bias. It has been reported that Hed1, a meiosis-specific protein in budding yeast, regulates Rad51-dependent recombination activity...
December 14, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27974735/deinococcus-rubrus-sp-nov-a-bacterium-isolated-from-antarctic-coastal-sea-water
#20
Sathiyaraj Srinivasan, Sangyong Lim, Jae-Hyun Lim, Hee-Young Jung, Myung Kyum Kim
Two Gram-staining-negative, red-pinkish, coccus-shaped, non-motile, and aerobic bacterial strains, designated Ant21(T) and Ant22, were isolated from the Antarctic coastal sea water. Strains Ant21(T) and Ant22 showed UVC and gamma radiation resistance. Phylogenetic analyses based on 16S rRNA gene sequences determined that these strains belonged to the genus Deinococcus. Through the analyses of the 16S rRNA gene sequences, strains Ant21(T) and Ant22 were found to have 97.7% and 97.8% similarity to Deinococcus marmoris DSM 12784T and 97...
December 14, 2016: Journal of Microbiology and Biotechnology
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