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Journal of Microbiology and Biotechnology

Chan-Woo Park, Yong-Seok Song, Hee Pom Lee, Jin Tae Hong, Do-Young Yoon
(E)-2-methoxy-4-(3-(4-methoxyphenyl)prop-1-en-1-yl)phenol (MMPP), derived from butenal, is a recently synthesized Maillard reaction (MR) product. Owing to its novelty, little is known about the function of MMPP. In this study, we elucidate the effects of MMPP on apoptosis in cervical cancer by using the HeLa cervical cancer cell line, which is widely used in cancer research. We observed that MMPP was cytotoxic to HeLa cells and induced activation of caspase-3, -8, and -9, without affecting the expression of the viral oncogenes E6 and E7...
May 17, 2017: Journal of Microbiology and Biotechnology
Tingting Wang, Lijun Cheng, Wenhao Zhang, Xiuhong Xu, Qingxin Meng, Xuewei Sun, Huajing Liu, Hongtao Li, Yu Sun
Composting is widely used to transform waste into valuable agricultural organic fertilizer. Anaerobic ammonium-oxidizing (Anammox) bacteria play an important role in the global nitrogen cycle, but their role in composting remains poorly understood. In the present study, the community structure, diversity, and abundance of anammox bacteria were analyzed using cloning and sequencing methods by targeting the 16S rRNA gene and the hydrazine oxidase gene (hzo) in samples isolated from compost produced from cow manure and rice straw...
May 17, 2017: Journal of Microbiology and Biotechnology
Dongbin Park, Yoonsoo Hahn
Complete genome sequences of three new plant RNA viruses, Spinach deltapartitivirus 1 (SpDPV1), Spinach amalgavirus 1 (SpAV1), and Spinach latent virus (SpLV), were identified from a spinach (Spinacia oleracea) transcriptome dataset. The RNA-dependent RNA polymerases (RdRps) of SpDPV1, SpAV1, and SpLV showed 72%, 53%, and 93% amino acid sequence identities with the homologous RdRp of the most closely related virus in each case, respectively, suggesting that SpDPV1 and SpAV1 were novel viruses. Sequence similarity and phylogenetic analyses revealed that SpDPV1 belonged to the genus Deltapartitivirus of the family Partitiviridae, SpAV1 to the genus Amalgavirus of the family Amalgaviridae, and SpLV to the genus Ilarvirus of the family Bromoviridae...
May 17, 2017: Journal of Microbiology and Biotechnology
Liang Qiu, Dong Yang, Xueying Tao, Jun Yu, Hua Xiong, Hua Wei
Trimethylamine N-oxide (TMAO), which is transformed from trimethylamine (TMA) through hepatic flavin-containing monooxygenases, can promote atherosclerosis. TMA is produced from dietary carnitine, phosphatidylcholine, and choline via the gut microbes. Previous works have shown that some small molecules, such as allicin, resveratrol, and 3,3-dimethyl-1-butanol, are used to reduce circulating TMAO levels. However, the use of bacteria as an effective therapy to reduce TMAO levels has not been reported. In the present study, 82 isolates were screened from healthy Chinese fecal samples on a basal salt medium supplemented with TMA as sole carbon source...
May 17, 2017: Journal of Microbiology and Biotechnology
Jimin Wi, Mun Sik Jeong, Hyo Jeong Hong
Hepatitis B virus (HBV) is a major cause of liver cirrhosis and hepatocellular carcinoma. With recent identification of HBV receptor, inhibition of virus entry has become a promising concept in the development of new antiviral drugs. To date, 10 HBV genotypes (A-J) have been defined. We previously generated two murine anti-preS1 monoclonal antibodies (mAbs), KR359 and KR127, that recognize amino acids (aa) 19-26 and 37-45, respectively, in the receptor binding site (aa 13-58, genotype C). Each mAb exhibited virus neutralizing activity in vitro, and a humanized version of KR127 effectively neutralized HBV infection in chimpanzees...
May 4, 2017: Journal of Microbiology and Biotechnology
Mina Kim, Yerin Jin, Hyun-Joo An, Jaehan Kim
The impact of overproduction of a heterologous protein on the expressed proteome of Lactococcus lactis was investigated. L. lactis IL1403 containing two near-identical plasmids driving high and low level expression of the green fluorescent protein (GFP) were examined via shotgun proteomics. Analysis of the two strains via high throughput LC-MS/MS proteomics identified the expression of 294 proteins. The relative amount of each protein in the proteome of both strains was determined by a label-free quantification using the spectral counting method...
May 4, 2017: Journal of Microbiology and Biotechnology
Jin-Hoon Jeong, Yoon-Soo Jo, Chang-Seon Park, Chang-Ho Kang, Jae-Seong So
In this study, the feasibility of introducing calcite-forming bacteria into concrete pavements to improve its mechanical performance was investigated. Lysinibacillus sphaericus WJ-8, which was isolated in a previous study and is capable of exhibiting high urease activity and calcite production, was used. When analyzed via scanning electron microscopy (SEM) and X-ray diffraction (XRD), WJ-8 showed a significant amount of calcite precipitation. Compressive strength of cement mortar mixed with WJ-8 cells and nutrient medium (urea with calcium lactate) increased by 10% compared with that of the controls...
May 4, 2017: Journal of Microbiology and Biotechnology
Bong-Kyu Song, Kyeng Min Kim, Kang-Duk Choi, Wan-Taek Im
The ginsenoside Rh2 has strong anti-cancer, anti-inflammatory, and anti-diabetic effects. However, the application of ginsenoside Rh2 is restricted because of the small amounts found in Korean white and red ginseng. To enhance the production of ginsenoside Rh2-MIX, comprising 20(S)-Rh2, 20(R)-Rh2, Rk2, and Rh3 as a 10-g unit, with high specificity, yield, and purity, a new combination of enzymatic conversion using the commercial enzyme Viscozyme L followed by acid treatment was developed. Viscozyme L treatment at pH 5...
May 4, 2017: Journal of Microbiology and Biotechnology
Hyoung-An Choi, Dae-Eun Cheong, Ho-Dong Lim, Won-Ho Kim, Mi-Hyoun Ham, Myung-Hwan Oh, Yuanzheng Wu, Hyun-Jae Shin, Geun-Joong Kim
Several medicinal plants are ethnomedically used in Korea as agents for treating infection, anti-inflammation, and pain relief. However, beyond typical inhibitory effects on cell growth, little is known about the potential anti-biofilm activity of these herbs, which may help to prevent cavities and maintain good oral health. This study aims to investigate the antimicrobial and anti-biofilm activity of the methanol extracts of 37 Korean medicinal plants against dental pathogens Streptococcus mutans and Candida albicans, which synergize their virulence so as to induce the formation of plague biofilms in the oral cavity...
May 4, 2017: Journal of Microbiology and Biotechnology
Sung Yong Jang, Jaeyoung Heo, Mi Ri Park, Min-Ho Song, Jong Nam Kim, Sung Ho Jo, Do-Youn Jeong, Hak Kyo Lee, Younghoon Kim, Sangnam Oh
Lactobacillus fermentum strain JDFM216, isolated from a Korean infant feces, possesses an enhancement of longevity and immune response on Caenorhabditis elegans host. To explore the molecular characteristics of strain JDFM216, we performed a whole-genome sequencing using PacBio system. The single circular DNA chromosome of 2,076,427 bp was completely assembled and 2,623 encoding sequences were predicted. Phylogenetically featured five genes possibly related to longevity and immune response of host were identified in L...
April 25, 2017: Journal of Microbiology and Biotechnology
Kang Wook Lee, Jae Min Shim, Zhuang Yao, Jeong A Kim, Hyun-Jin Kim, Jeong Hwan Kim
To develop starters for the production of functional foods or materials, lactic acid bacteria producing γ-aminobutyric acid (GABA) were screened from jeotgals, Korean fermented sea foods. One isolate producing high amount of GABA from monosodium L-glutamate (MSG) was identified as Enterococcus avium by 16S rRNA gene sequencing. E. avium M5 produced 18.47 ± 1.26 mg/ml GABA when incubated for 48 h at 37°C in MRS broth with MSG (3%, w/v). A gadB gene encoding glutamate decarboxylase (GAD) was cloned and overexpressed in E...
April 25, 2017: Journal of Microbiology and Biotechnology
Hyun-Hee Lee, Naru Kang, Inmyoung Park, Jungwook Park, Inyoung Kim, Jieun Kim, Namgyu Kim, Jae-Yun Lee, Young-Su Seo
Cordyceps militaris, a member of Ascomycota, a mushroom referred to as caterpillar Dong-chung-ha-cho, is commercially valuable because of its high content of bioactive substances including cordycepin and its potential for artificial cultivation. Cordycepin (3'-deoxyadenosine) is highly associated with the pharmacological effects of C. militaris. C. militaris is heterothallic in that two mating type loci, idiomorph MAT1-1 and MAT1-2, exist discretely in two different spores. In this study, nine C. militaris strains were mated with each other to prepare new strains that produced a larger amount of cordycepin than the parent strains...
April 25, 2017: Journal of Microbiology and Biotechnology
Shalem Raj Padakandla, Jong-Chan Chae
A rod shaped, aerobic, Gram-stain-negative, and motile bacterium, strain AR2(T), was isolated from a water sample of Yeongsan river, Republic of Korea. Strain AR2(T) clustered closely with the members of the genus Chitinimonas and showed the highest 16S rRNA gene sequence similarity with Chitinimonas prasina LY03(T) (96.4%), Chitinimonas viridis HMD2169(T) (96.4%), Chitinimonas taiwanensis cf(T) (96.2%), and Chitinimonas koreensis R2A43-10(T) (94.2%). The predominant fatty acids of strain AR2(T) were identified to be summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c), C16:0, and C10:03-OH...
April 21, 2017: Journal of Microbiology and Biotechnology
Adi Setyo Purnomo, Khoirul Ashari, Farizha Triyogi Hermansyah
DDT (1,1,1-trichloro-2,2-bis(4-chlorophenyl) ethane) is one of the organic synthetic pesticides that has many negative effects for human health and the environment. The purpose of this study was to investigate the synergistic effect of mixed cutures of white-rot fungus, Pleurotus ostreatus, and biosurfactant-producing bacteria, Pseudomonas aeruginosa and Bacillus subtilis, on DDT biodegradation. Bacteria was added into P. ostreatus culture (mycelial wet weight on average by 8.53 g) in concentrations of 1, 3, 5, and 10 mL (1 mL ≈ 1...
April 21, 2017: Journal of Microbiology and Biotechnology
Hyung-Seok Oh, Chang Soo Lee, Ankita Srivastava, Hee-Mock Oh, Chi-Yong Ahn
Geosmin and 2-methylisoborneol (2-MIB), responsible for earthy or musty smell, are a major concern for safe drinking water supplies. This study investigated the effects of environmental factors on odorous compounds production and cell growth in cyanobacterial strains. Anabaena sp. FACHB-1384, a 2-MIB producer, was sensitive to low temperature (<20°C). However, geosmin producers, Anabaena sp. Chusori and Anabaena sp. NIER, were sensitive to high light intensity (>100 μmol/m²/sec), but not to low temperature...
April 21, 2017: Journal of Microbiology and Biotechnology
Hyong Seok Park, Jungwoo Yang, Hee Jung Choi, Kyoung Heon Kim
Microwave sterilization was performed to inactivate the spores of biofilms of Bacillus cereus involved in foodborne illness. The sterilization conditions, such as the amount of water biofilms were immersed in, temperature, and treatment time, were optimized after 15 runs based on the Box-Behnken design. The sterilization conditions, such as the amount of water and the operating temperature and treatment time were optimized using statistical analysis based on 15 runs experimental results designed by the Box- Behnken method...
April 20, 2017: Journal of Microbiology and Biotechnology
Manomi Sarasan, Jayesh Puthumana, Neema Job, Jeonghoon Han, Jae-Seong Lee, Rosamma Philip
Endophytic fungi have currently been acknowledged, as the most promising source of bioactive compounds for drug discovery and considerable progress has been made in exploring their diversity, species richness, and bioprospecting. Fungal endophytes from unique environmental settings offer a pool of potentially useful medicinal entities. Due to the constant stresses imposed on macroalgae by marine environment, it is believed that algae and their associated endophytic symbionts represent a good source of structurally diverse bioactive secondary metabolites...
April 5, 2017: Journal of Microbiology and Biotechnology
Tae Gyu Nam, Bong Han Lee, Hyo-Kyoung Choi, Ahmad Rois Mansur, Sang Gil Lee, Dae-Ok Kim
Rhus verniciflua Stokes (RVS), an herbal medicine found in East Asia, was extracted and further fractionated to investigate its antioxidant capacity and neuroprotective effects. The RVS ethyl acetate (EtOAc) fraction had the highest level of total phenolics and antioxidant capacity among all solvent fractions tested. Pretreatment of PC-12 cells with the EtOAc fraction effectively attenuated H2O2-induced oxidative damage. Furthermore, the EtOAc fraction significantly attenuated caspase-3 activity, resulting in inhibition of H2O2-induced apoptosis...
April 5, 2017: Journal of Microbiology and Biotechnology
Sung-Jong Jeon, Su-Jin Lim
Marasmius scorodonius secretes an extracellular laccase in potato dextrose broth, and this enzyme was purified up to 206-fold using (NH4)2SO4 precipitation and a Hi-trap Q Sepharose column. The molecular weight of the purified laccase was estimated to be ~67 kDa by SDS-PAGE. The UV/vis spectrum of the enzyme is nontypical for laccases, and metal content analysis revealed that the enzyme contains 1 mole of Fe and Zn and 2 moles of Cu per mole of protein. The optimal pH for the enzymatic activity was 3.4, 4.0 and 4...
April 5, 2017: Journal of Microbiology and Biotechnology
Cheol-Ho Jang, Gayeon Lee, Yong-Cheol Park, Kyoung Heon Kim, Do Yup Lee
Understanding phosphorus metabolism in photosynthetic organisms is important in that it is closely associated with enhanced crop productivity and pollution management for natural ecosystems (e.g. algal blooming). Accordingly, we exploited highly time-resolved metabolic responses to different levels of phosphate deprivation in C. reinhardtii, a photosynthetic model organism. We conducted non-targeted primary metabolite profiling using gas-chromatography time-of-flight mass spectrometric analysis. Primarily, we systematically identified main contributors to degree-wise responses corresponding to the deprivation levels of phosphate...
April 3, 2017: Journal of Microbiology and Biotechnology
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