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Journal of Microbiology and Biotechnology

Ji Eun Ahn, Hangeun Kim, Dae Kyun Chung
Lipoteichoic acid isolated from Lactobacillus plantarum K8 (pLTA) alleviates lipopolysaccharide (LPS)-induced excessive inflammation through inhibition of TNF-α and interleukin (IL)-6. In addition, pLTA increases the survival rate of mice in a septic shock model. In the current study, we have found that pLTA contributes to homeostasis through regulation of pro- and anti-inflammatory cytokine production. In detail, pLTA decreased the production of IL-10 by phorbol-12-myristate-13-acetate (PMA)-differentiated THP-1 cells stimulated with prostaglandin E2 (PGE-2) and LPS...
November 2, 2018: Journal of Microbiology and Biotechnology
Ayeong Lee, Ji Yea Kim, Jina Heo, Dae-Hyun Cho, Hee-Sik Kim, In-Sook An, Sungkwan An, Seunghee Bae
Abnormal melanin synthesis results in several hyperpigmentary disorders such as freckles, melanoderma, age spots, and other hyperpigmentation syndromes. In this study, we investigated the anti-melanogenic effects of an extract from the microalgae Chlamydomonas reinhardtii (CE) and potential mechanisms responsible for its inhibitory effect in B16F10, normal human epidermal melanocyte cells, and human skin-equivalent models. The CE extract showed significant dose-dependent inhibitory effects on α-melanocyte-stimulating hormone-induced melanin synthesis in cells...
November 2, 2018: Journal of Microbiology and Biotechnology
Setu Bazie Tagele, Hyun Gu Lee, Sang Woo Kim, Youn Su Lee
In this study, strain KNU17Pc1 was tested for its antifungal activity against Rhizoctonia solani AG-1(IA), which causes banded leaf and sheath blight (BLSB) of maize. KNU17Pc1 was tested further for its broad-spectrum antifungal activity and in vitro plant growth promoting (PGP) traits. In addition, the in vivo effects of KNU17Pc1 on reduction of BLSB severity and seedling growth promotion of two maize cultivars under greenhouse conditions were investigated. On the basis of multilocus sequence analysis (MLSA), KNU17Pc1 was confirmed as P...
November 2, 2018: Journal of Microbiology and Biotechnology
Lijun Wang, Baihua Zhou, Xue Zhou, Yang Wang, Hongwei Wang, Shengying Jia, Zhipeng Zhang, Chao Chu, Jianjun Mu
Statins are a class of lipid lowering drugs commonly used in the prevention of cardiovascular diseases. However, statin therapy present many limitations, which lead to an increased interest in non-drug therapies, such as probiotics, to improve blood cholesterol levels. Indeed, probiotic strains such as Lactobacillus acidophilus have been found to improve blood lipid profiles, especially in reducing total cholesterol and LDL-C levels. In this study, we established a high-cholesterol rat model and studied the effect of Lactobacillus acidophilus administration alone or in conjunction with rosuvastatin...
November 2, 2018: Journal of Microbiology and Biotechnology
Jing Liu, Guilian Yang, Xing Gao, Zan Zhang, Yang Liu, Xin Yang, Chunwei Shi, Qqiong Liu, Yanlong Jiang, Chunfeng Wang
As a member of lactic acid bacteria species, Lactobacillus plantarum ( L. plantarum ) has been used extensively for vaccine delivery previously. Considering to the critical role of dendritic cells in stimulating host immune response, in this study, we constructed a novel CD11c targeting L. plantarum strain with surface displayed the variable fragments of anti-CD11c single chain antibody (scFv-CD11c). The newly designed L. plantarum strain, named 409-aCD11c, could adhere and invade more efficiently to bone marrow-derived DCs (BMDCs) in vitro due to the specific interaction between scFv-CD11c and CD11c located on the surface of BMDCs...
November 2, 2018: Journal of Microbiology and Biotechnology
Hemraj Rimal, Sang-Cheol Yu, Byeongsan Lee, Young-Soo Hong, Tae-Jin Oh
Geldanamycin and its derivatives, inhibitor of heat shock protein 90, are considered as potent anticancer drug, and their biosynthetic pathway has not yet been fully elucidated. The key step of conversion of 4,5-dihydrogeldanamycin to geldanamycin was expected to catalyse by a P450 monooxygenase, Gel16. The adequate bioconversions by cytochrome P450 mostly rely upon its interaction with redox partners. Several numbers of ferredoxin and ferredoxin reductase are available in a genome of organism, but only few suitable partners can operate in full efficiency...
November 2, 2018: Journal of Microbiology and Biotechnology
Ayaz Anwar, Ruqaiyyah Siddiqui, Muhammad Raza Shah, Naveed Ahmed Khan
Parasitic infections have remained a significant burden on human and animal health. In part, this is due to lack of clinically-approved novel antimicrobials and a lack of interest by the pharmaceutical industry. An alternative approach is to modify existing clinically-approved drugs for efficient drugs delivery formulations to ensure minimum inhibitory concentration is achieved at the target site. Nanotechnology offers the potential to enhance the therapeutic efficacy of drugs through modification of nanoparticles with ligands...
November 2, 2018: Journal of Microbiology and Biotechnology
Byung-Joo Park, Soon-Tag Jung, Chang-Sun Choi, Jin-Jong Myoung, Hee-Seop Ahn, Sang-Hoon Han, Yong-Hyun Kim, Hyeon-Jeong Go, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, In-Soo Choi
Norovirus is the most common cause of acute gastroenteritis. Its pathogenesis is poorly understood owing to the difficulty of establishing viral infection in animal models. Here, post-weaning gnotobiotic pigs were infected with human norovirus genogroup II genotype 4 (HuNoV GII.4) to investigate the pathogenesis and replication of the virus. Three groups of four pigs were infected with 1×105 , 1×106 , or 1×107 genomic equivalent (GE) copies of HuNoV GII.4. Four pigs were used as negative controls. Blood and rectal swab samples were collected after viral infection, and gross legions were examined after necropsy...
November 2, 2018: Journal of Microbiology and Biotechnology
Jae Pil Lee, Eun-Sun Shin, Min Yeol Cho, Kyung-Dong Lee, Hoon Kim
An endo-β-1,4-glucanase gene, cel5 L, was cloned using the shot-gun method from Bacillus sp.. The gene, which contained a predicted signal peptide, encoded a protein of 496 amino acid residues, and the molecular mass of the mature Cel5L was estimated to be 51.8 kDa. Cel5L contained a catalytic domain of glycoside hydrolase (GH) family 5 and a carbohydrate-binding module family 3 (CBM_3). Chromatography using HiTrap Q and CHT-II resulted in the isolation of two truncated forms corresponding to 50 (Cel5L-p50) and 35 kDa (Cel5L-p35, CBM_3-deleted form)...
October 26, 2018: Journal of Microbiology and Biotechnology
Do-Won Jeong, Byunghoon Lee, Hyundong Lee, Keuncheol Jeong, Mihyun Jang, Jong-Hoon Lee
In 2015, Bacillus paralicheniformis was separated from B. licheniformis on the basis of phylogenomic and phylogenetic studies, and urease activity was reported as a phenotypic property able to differentiate between the two species. Subsequently, we have found that the urease activity of B. paralicheniformis is strain-specific, and does not reliably discriminate between species, as strains having the same urease gene cluster were identified in B. licheniformis and B. sonorensis , the closest relatives of B. paralicheniformis ...
October 26, 2018: Journal of Microbiology and Biotechnology
Yun-Qing Li, Qing-Jie Xue, Yuan-Yuan Yang, Hui Wang, Xiu-Zhen Li
The spatial landmark protein Bud8 plays a crucial role in bipolar budding in the budding yeast Saccharomyces cerevisiae . The unconventional yeast Yarrowia lipolytica can also bud in a bipolar pattern, but is evolutionarily distant from S. cerevisiae . It encodes the protein YALI0F12738p, which shares the highest amino acid sequence homology with S. cerevisiae Bud8, sharing a conserved transmembrane domain at the C-terminus. Therefore, we named it YlBud8. Deletion of YlBud8 in Y. lipolytica causes cellular separation defects, resulting in budded cells remaining linked with one another as cell chains or multiple buds from a single cell, which suggests that YlBud8 may play an important role in cell separation, which is distinct from the function of Bud8 in S...
October 26, 2018: Journal of Microbiology and Biotechnology
Muhammad Waqas, Huyen Trang Trinh, Sungeun Lee, Dae-Hwan Kim, Sang Yeol Lee, Kevin K Choe, Chongsuk Ryou
Based on previous studies reporting anti-prion activity of poly-L-lysine and poly-L-arginine, cationic poly-L-ornithine (PLO) and poly-L-histidine (PLH), anionic poly-L-glutamic acid (PLE) and uncharged poly-L-threonine (PLT) were investigated in cultured cells chronically infected by prions to determine anti-prion efficacy. While PLE and PLT did not alter the level of PrPSc , PLO and PLH exhibited potent PrPSc inhibition in ScN2a cells. These results suggest that anti-prion activity of poly-basic amino acids is correlated with cationicity of their functional groups...
October 26, 2018: Journal of Microbiology and Biotechnology
Hongjuan Jing, Huanhuan Liu, Zhang Lu, Jie Gao, Haoran Song, Xiaorong Tan
Ethanol accumulation inhibited the growth of Saccharomyces cerevisiae during wine fermentation. Autophagy and the release of reactive oxygen species (ROSs) were also induced under ethanol stress. However, the relation between autophagy and ethanol stress was still unclear. In the study, expression of autophagy genes ATG1 and ATG8 and production of ROS under ethanol treatment in yeast were measured. The results showed that ethanol stress very significantly induced expression of ATG1 and ATG8 genes and the production of peroxide hydrogen (H₂O₂) and superoxide anion (O₂·- )...
October 26, 2018: Journal of Microbiology and Biotechnology
Sajid Ali, Soon-Ki Park, Won-Chan Kim
Several genetic strategies have been proposed for the successful transformation and expression of microbial transgenes in model and crop plants. Here, we bring into focus the prominent applications of microbial transgenes in plants for the development of disease resistance; mitigation of stress conditions; augmentation of food quality; and use of plants as " bioreactors " for the production of recombinant proteins, industrially important enzymes, vaccines, antimicrobial compounds, and other valuable secondary metabolites...
October 26, 2018: Journal of Microbiology and Biotechnology
Juyoung Byun, Sena Yoon, Yunji Jeong, Uitaek Oh, Sujin Cho, Jeongsoo Park, Yongsu Jeong, Kwanghee Baek, Jaeseung Yoon
Development of stable rCHO cell lines is still time-consuming and labor-intensive, although it is a critical step in commercial development of recombinant antibodies. The current work demonstrates, for the first time, that electroporation of CHO cells with DMSO can enhance stable expression of recombinant antibodies in rCHO cells. Eelectroporation with DMSO resulted in an average 3.7-fold and 2.8-fold increases in expression levels of aflibercept and pembrolizumab, respectively, in pools of stable rCHO cells...
October 26, 2018: Journal of Microbiology and Biotechnology
Wen-Wen Zhang, Xue-Fei Zhou, Ya-Lei Zhang, Peng-Fei Cheng, Rui Ma, Wen-Long Cheng, Hua-Qiang Chu
The natural astaxanthin mainly derives from the microalgae producer, Haematococcus pluvialis . The induction of nitrogen starvation and high light intensity is particularly significant for boosting astaxanthin production. However, the different responses of light intensity and nitrogen starvation should be analyzed for biomass growth and astaxanthin accumulation. The results showed that the highest level of astaxanthin was achieved in nitrogen starvation, which was 1.64 times higher than the control group at 11 days...
October 26, 2018: Journal of Microbiology and Biotechnology
So-Ra Ko, Young-Ki Lee, Ankita Srivastava, Seung-Hwan Park, Chi-Yong Ahn, Hee-Mock Oh
Fusaricidin analogs, produced by Paenibacillus polymyxa , were tested for selective control of a major bloom-forming cyanobacterium Microcystis sp. Fusaricidin (A and B mixtures) and four analogs were isolated from P. polymyxa E681 and investigated for their inhibition of cyanobacterial cell growth. Among the four fusaricidin analogs, fraction 915 Da (designated as Fus901) showed growth inhibition activity for Microcystis aeruginosa but not for Anabaena variabilis and Scenedesmus acutus . Microcystin concentration decreased up to 70% and its content per cell also decreased over 50% after 3 days...
October 26, 2018: Journal of Microbiology and Biotechnology
So-Jin Yang, Jin-A Ko, Hae-Soo Kim, Min-Ho Jo, Ha-Nul Lee, Bo-Ram Park, YoungMin Kim
Cycloisomaltooligosaccharide glucanotransferase (CITase) was isolated from alkaliphilic Paenibacillus daejeonensis via an amino acid homology search for the reported CITase. The recombinant alkaliphilic CITase (PDCITase) from P. daejeonensis was expressed in an Escherichia coli expression system and purified as a single protein band of 111 kDa. PDCITase showed optimum activity at pH 8.0 and retained 100% of activity within a broad pH range (7.0-11.5) after 18 h, indicating alkaliphilic or alkalistable CITase properties...
October 26, 2018: Journal of Microbiology and Biotechnology
David Quintero, Jamie Carrafa, Lena Vincent, Hee Jong Kim, James Wohlschlegel, David Bermudes
Sunflower trypsin inhibitor (SFTI) is a 14 amino acid bicyclic peptide that contains a single internal disulfide bond. We initially constructed chimeras of SFTI with N-terminal secretion signals from the Escherichia coli and the Pseudomonas aeruginosa , but only detected small amounts of protease inhibition resulting from these constructs. A substantially higher degree of protease inhibition was detected from a C-terminal SFTI fusion with E. coli YebF, which radiated more than a centimeter from a single colony of E...
October 19, 2018: Journal of Microbiology and Biotechnology
Hye Jeong Gil, Sunmin Lee, Digar Singh, ChoongHwan Lee
In this study, we investigated the altered enzymatic activities and metabolite profiles of koji fermented using varying permutations of Aspergillus oryzae and/or Bacillus amyloliquefaciens . Notably, the protease and β -glucosidase activities were manifold increased in co-inoculated (CO) koji samples (co-inoculation of A. oryzae and B. amyloliquefaciens ). Furthermore, gas chromatography-mass spectrometry (GC-MS)-based metabolite profiling indicates that levels of amino acids, organic acids, sugars, sugar alcohols, fatty acids, nucleosides, and vitamins were distinctly higher in CO, SA (sequential inoculation of A...
October 19, 2018: Journal of Microbiology and Biotechnology
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