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Journal of Microbiology and Biotechnology

Jin-Moo Lee, JunWon Kim, Ilhwan Ryu, Hye-Min Woo, Tae Gyun Lee, Woong Jung, Sanggyu Yim, Yong-Joo Jeong
The surface protein hemagglutinin (HA) mediates the attachment of influenza virus to host cells containing sialic acid and thus facilitate viral infection. Therefore, HA is considered as a good target for the development of diagnostic tools for influenza virus. Previously, we reported the isolation of single-stranded aptamers that can distinguish influenza subtype H1 from H5. In this study, we described a method for the selective electrical detection of H1 using the isolated aptamer as a molecular probe. After immobilization of the aptamer on Si wafer, enzyme-linked immunosorbent assay (ELISA) and field emission scanning electron microscope (FE-SEM) showed that the immobilized aptamer specifically bound to only the H1 subtype but not the H5 subtype...
September 15, 2017: Journal of Microbiology and Biotechnology
Jin-Moo Lee, Jin-Beom Cho, Hee-Chul Ahn, Woong Jung, Yong-Joo Jeong
We have discovered a novel chemical compound, (E)-3-(furan-2-yl)-N-(4-sulfamoylphenyl)acrylamide, that suppresses the enzymatic activities of SARS coronavirus helicase. To determine the inhibitory effect, ATP hydrolysis and double-stranded DNA unwinding assays were performed in the presence of various concentrations of the compound. Through these assays, we have obtained IC50 values of 2.09 ± 0.30 µM (ATP hydrolysis) and 13.2 ± 0.9 µM (DNA unwinding), respectively. Moreover, we have found that the compound did not have any significant cytotoxicity when 40 µM of the compound was used...
September 15, 2017: Journal of Microbiology and Biotechnology
Bo-Min Kim, Ha Young Choi, Geon-Woo Kim, Chang-Ji Zheng, Young-Ho Kim, Won-Gon Kim
FtsZ, a bacterial cell-division protein, is an attractive antibacterial target. In the screening for an inhibitor of Staphylococcus aureus FtsZ, madurahydroxylactone (1) and its related derivatives 2-5 were isolated from Nonomuraea sp. AN1000570. Compound 1 inhibited S. aureus FtsZ with an IC50 of 53.4 µM and showed a potent antibacterial activity against S. aureus and MRSA with an MIC of 1 µg/mL, while 2-5 were weak or inactive. Importantly, 1 induced cell elongation in the cell division phenotype assay, while 2-5 didn't...
September 15, 2017: Journal of Microbiology and Biotechnology
Yincong Li, Seockmo Ku, Myeong Soo Park, Zhipeng Li, Geun Eog Ji
Recently, soy bean isoflavone aglycones (i.e. daidzein and genistein) and γ-aminobutyric acid (GABA) have begun to receive considerable consumer attention due to their potential as nutraceuticals. To produce these ingredients, multiple microorganisms and their enzymes are commonly used for catalysis in the nutraceutical industry. In this work, we introduce a novel fermentation process that uses whole cell biocatalysis to accelerate γ-aminobutyric acid and isoflavone aglycones production in doenjang (traditional Korean soybean paste)...
September 15, 2017: Journal of Microbiology and Biotechnology
Soojin Park, Jae-Won Huh, Taekil Eom, Naeun Na, Youngjeon Lee, Ju-Sung Kim, Sun-Uk Kim, Insop Shim, Sang-Rae Lee, Ekyune Kim
The main pathological hallmark of Alzheimer's disease is the deposition of amyloid-β peptide (Aβ) in the brain. Aβ has been widely used to mimic several aspects of Alzheimer's disease. However, several characteristics of amyloid-induced Alzheimer's disease pathology are not well established, especially in mice. The present study aimed to develop a new Alzheimer's disease model by investigating how amyloid beta can be effectively aggregated using prokaryotes and eukaryotes. To express the Aβ42 complex in HEK293 cells, we cloned the Aβ42 region repeatedly and incorporated the resulting construct into a eukaryotic expression vector...
September 15, 2017: Journal of Microbiology and Biotechnology
Joon Seob Seong, Song Hua Xuan, So Hyun Park, Keon Soo Lee, Young Min Park, Soo Nam Park
Lespedeza cuneata G. Don is a traditional herb that has been shown to associate with multiple biological activities. In this study, we investigated the antioxidative/antiaging activities and performed an active component analysis of the non-fermented and fermented (using Lactobacillus pentosus) extracts of Lespedeza cuneata G. Don. The antioxidative activities of the fermented extract were higher than those of non-fermented extracts. The elastase inhibitory activity, inhibitory effects on UV-induced MMP-1 expression, and ability to promote type I procollagen synthesis were investigated in Hs68 human fibroblasts cells...
September 15, 2017: Journal of Microbiology and Biotechnology
Zhen-Quan Yang, Yu Xue, Mi Zhang, Sheng-Qi Rao, Lu Gao, Yong-Qi Yin, Da-Wei Chen, Xiao-Hui Zhou, Xin-An Jiao
Piliated Lactobacillus rhamnosus (pLR) strains possess higher adherent capacity than non-piliated strains. The objective of this study was to isolate and characterise probiotic pLR strains in human faecal samples. To this end, mouse polyclonal antiserum (anti-SpaA) against the recombinant pilus protein (SpaA) of L. rhamnosus strain GG (LGG) was prepared and tested for its reactivity and specificity. With the anti-SpaA, a method combining the de Man Rogosa and Sharpe (MRS) agar plating separation and colony immunoblotting (CIB) was developed to isolate pLR from 124 human faecal samples...
September 15, 2017: Journal of Microbiology and Biotechnology
Soon Yong Park, Ae-Jin Jeong, Geun-Young Kim, Ara Jo, Joo Eon Lee, Sun-Hee Leem, Joung-Hahn Yoon, Sang Kyu Ye, Jin Woong Chung
Mesenchymal stem cells (MSCs) have been suggested as a primary candidate for cell therapy applications because they have self-renewal and differentiation capabilities. Although they can be expanded in ex vivo system, clinical application of these cells is still limited because they poorly survive and undergo senescence or apoptosis when transplanted and exposed to environmental factors such as oxidative stress. Thus, reducing oxidative stress is expected to improve the efficacy of MSCs therapy. A milk protein, lactoferrin is the multifunctional iron-binding glycoprotein and plays various roles including reduction of oxidative stress...
September 5, 2017: Journal of Microbiology and Biotechnology
Huynh Tan Hop, Lauren Togonon Arayan, Alisha Wehdnesday Bernardo Reyes, Tran Xuan Ngoc Huy, Eun Jin Baek, WonGi Min, Hu Jang Lee, Chun Hee Lee, Man Hee Rhee, Suk Kim
In this study, we evaluated the inhibitory effect of a rice bran mixture extract (RBE) on Brucella (B.) abortus pathogenesis in professional (RAW 264.7) and non-professional (HeLa) phagocytes. We fermented the rice bran mixture and then extracted with 50% ethanol followed by gas chromatography-mass spectrometry to identify components in RBE. Our results clearly showed that RBE caused a significant reduction in the adherence of B. abortus in both cell lines. Furthermore, analysis of phagocytic signaling proteins by Western blot revealed that RBE pretreatment resulted in inhibition of phosphorylation of JNK, ERK and p38, leading to decline of internalization compared with the controls...
September 5, 2017: Journal of Microbiology and Biotechnology
Tae-Hun Lee, Jae-Kyung Jang, Hyun-Woo Kim
Mixotrophic microalgal growth gives a great premise for wastewater treatment based on photoautotrophic nutrient utilization and heterotrophic organic removal while producing renewable biomass. There remains a need for a control strategy to enrich them in a photobioreactor. This study performed a series of batch experiments using a mixotroph, Chlorella sorokiniana, to characterize optimal guidelines of mixotrophic growth based on the statistical design of experiment. Using a central composite design, this study evaluated how temperature and light irradiance is associated with the CO₂ capture and organic carbon respiration through biomass production and ammonia removal kinetics...
September 5, 2017: Journal of Microbiology and Biotechnology
Mee-Jung Han
The Escherichia coli K-12 and B strains are among the most frequently used bacterial hosts for scientific research and biotechnological applications. However, omics analyses have revealed that E. coli K-12 and B exhibit notably different genotypic and phenotypic attributes, even though they were derived from the same ancestor. In a previous study, we identified a limited number of proteins from the two strains using two-dimensional gel electrophoresis and tandem mass spectrometry (MS/MS). In this study, an in-depth analysis of the physiological behavior of the E...
September 5, 2017: Journal of Microbiology and Biotechnology
Nam Keun Lee
The aim of this study was to examine the efficiency of Rhodosporidium toruloides as a new tool to evaluate the triglyceride (TG) reduction effects of anti-obesity candidate materials. Unfermented and fermented persimmon leaf hot water extracts (UFPLE and FPLE) were used as anti-obesity agents. The content of TG in R. toruloides treated with FPLE was less than those with UFPLE by about 11% (p < 0.05) compared to the control (R. toruloides incubated in YPD medium without the agents). Fat reduction in 3T3-L1 cells achieved FPLE was about 13% higher than that achieved by UFPLE...
September 5, 2017: Journal of Microbiology and Biotechnology
Keum-Ok Moon, Heejin Park, Myungsoo Joo, Ki-Tae Ha, Nam-In Baek, Cheon-Seok Park, Jaeho Cha
In this study, we synthesized a glycosylated derivative of caffeic acid phenethyl ester (CAPE) using the amylosucrase from Deinococcus geothermalis with sucrose as a substrate and examined its solubility, chemical stability, and anti-inflammatory activity. Nuclear magnetic resonance spectroscopy showed that the resulting glycosylated CAPE (G-CAPE) was the new compound caffeic acid phenethyl ester-4-O-α-D-glucopyranoside. G-CAPE was 770 times more soluble than CAPE and highly stable in Dulbecco's modified Eagle's medium and buffered solutions, as estimated by its half-life...
September 5, 2017: Journal of Microbiology and Biotechnology
Hyemyoung Ahn, Jeongho Jeong, Knowledge Mudhibadi Moyo, Yungsun Ryu, Bokkee Min, Seong Ho Yun, Hwa Yeon Kim, Wooki Kim, Gwang Woong Go
Korean red pine (Pinus densiflora) bark extract, PineXol (PX), was investigated on its potential anti-oxidant and anti-inflammation effects in vitro. It was hypothesized that PX treatment (25-150 µg/mL) would reduce the lipid synthesis in HepG2 hepatocytes as well as lipid accumulation in 3T3-L1 adipocytes. Hepatocytes' intracellular triglycerides and cholesterol were decreased in the PX 150 µg/mL treatment group compared to the control (p<0.05). Consequently, de novo lipogenic proteins; acetyl-CoA carboxylase 1, stearoyl-CoA desaturase 1, elongase of very long chain fatty acids 6, glycerol-3-phosphate acyltransferase 1, and sterol regulatory element-binding protein 1, were significantly decreased in hepatocytes by PX 150 µg/mL treatment compared to the control (p<0...
September 5, 2017: Journal of Microbiology and Biotechnology
Sukyung Kim, Hoonhee Seo, Hafij Al Mahmud, Md Imtiazul Islam, Yong-Sik Kim, Jiwon Lyu, Kung-Woo Nam, Byung-Eui Lee, Kee-In Lee, Ho-Yeon Song
DFC-2, a methyl 5-[2-(dimethylamino)ethoxy]-7,12-dioxo-7,12-dihydrodinaphtho[1,2-b:2',3'-d]furan-6-carboxylate, is reported to have antitubercular effects against Mycobacterium tuberculosis. At concentrations ranging from 0.19 to 0.39 µg/ml, DFC-2 inhibited both drug-susceptible and -resistant strains of M. tuberculosis. Microarray analyses were employed to gain insights into the molecular mechanisms of DFC-2's action in M. tuberculosis. The most affected functional gene category was "lipid biosynthesis", which is involved in mycolic acid synthesis...
September 5, 2017: Journal of Microbiology and Biotechnology
Gi Hoon Jung, Eun Seob Lim, Min-Ah Woo, Joo Young Lee, Joo-Sung Kim, Hyun-Dong Paik
Campylobacter jejuni and C. coli are important foodborne pathogenic bacteria, especially in poultry meat. In this study, the presence of extracellular DNase activity was investigated for biofilm-deficient Campylobacter strains versus biofilm-forming Campylobacter strains isolated from chickens to understand the relation between extracellular DNase activity and biofilm formation. Biofilm-forming reference strain, C. jejuni NCTC11168, was co-incubated with biofilm non-forming strains isolated from raw chickens or their supernatants...
September 5, 2017: Journal of Microbiology and Biotechnology
Tae-Yoon Kim, Jin-Jae Lee, Bong-Soo Kim, Sang Ho Choi
Sea cucumber (Apostichopus japonicus) is a popular seafood source in Asia, including South Korea, and its consumption has recently increased with recognition of its medicinal properties. However, because raw sea cucumber contains various microbes, its ingestion can cause foodborne illness. Therefore, analysis of the microbiota in the whole body of sea cucumber can extend our understanding of foodborne illness caused by microorganisms and help to better manage products. We collected 40 sea cucumbers from four different sites in August and November, which are known as the maximum production areas in Korea...
August 31, 2017: Journal of Microbiology and Biotechnology
Hoa Thi Nguyen, Nan Hee Yu, Ae Ran Park, Hae Woong Park, In Son Kim, Jin-Cheol Kim
This study aimed to isolate and characterize an antibacterial metabolite from Pharbitis nil seeds and investigate antibacterial activity against various plant pathogenic bacteria. The methanol extract of P. nil seeds showed the strongest activity against Xanthomonas arboricola pv. pruni (Xap) with minimum inhibition concentration (MIC) value of 250 μg/ml. Among the three solvent layers obtained from the methanol extract of P. nil seeds, only butanol layer displayed the activity with MIC value of 125 μg/ml against Xap...
August 31, 2017: Journal of Microbiology and Biotechnology
Yongsheng Tian, Jing Xu, Lei Chen, Xiao-Yan Fu, Ri-He Peng, Quan-Hong Yao
Thermostability is an important property of xylanase because high temperature is required for its applications, such as wood pulp bleaching, baking, and animal feedstuff processing. In this study, XynB from Thermobacillus composti, a moderately thermophilic Gram-negative bacterium, was modified via site-directed mutagenesis based on 3D structure to obtain thermostable xylanase, and the properties of this enzyme were analyzed. Results revealed that the half-life of xylanase at 65 °C increased from 10 min to 50 min after a disulfide bridge was introduced between α-helix and its adjacent β-sheet at S98 and N145...
August 31, 2017: Journal of Microbiology and Biotechnology
Meisam Jeirani Khameneh, Farzaneh Moshiri, Soheil Keyhan Falasafi, Alireza Zomorodipour
Secretion efficiency of a protein in a SEC-type secretion system is mainly determined by an N-terminal signal peptide and its combination with its cognate protein. Five signal peptides, including; two synthetic Sec-type and three Bacillus licheniformis alpha-amylase derived signal peptides were compared for periplasmic expression of the human growth hormone (hGH) in E. coli. Based on in silico predictions on the signal peptides' cleavage efficiencies and their corresponding mRNA secondary structures, a number of amino acid substitutions and silent mutations were considered in the modified signal sequences...
August 31, 2017: Journal of Microbiology and Biotechnology
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