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Journal of Microbiology and Biotechnology

Nguyen Hong Phuong, Chaewon Kwak, Chang-Kyu Heo, Eun Wie Cho, Jihyun Yang, Haryoung Poo
Influenza, which is a highly contagious disease caused by the influenza A virus, continues to be a major health concern worldwide. Although the accurate and early diagnosis of influenza virus infection is important for controlling the spread of this disease and rapidly initiating anti-viral therapy, the current influenza diagnostic kits are limited by their low sensitivity. In this study, we developed several new influenza nucleoprotein (NP)-specific monoclonal antibodies (mAbs) and compared their sensitivity and specificity with those of commercially available anti-NP mAbs...
April 12, 2018: Journal of Microbiology and Biotechnology
Jong Min Oh, Jae Pil Lee, SeungCheol Baek, Yang Do Jo, Hoon Kim
A cellulolytic fungus YDJ14 was is 31 olated from compost and identified as an Aspergillus sp. strain. Three extracellular β-glucosidases, BGL-A1, BGL-A2, and BGL-A3, were separated using ultrafiltration, ammonium sulfate fractionation, and High-Q chromatography. The molecular masses of the three enzymes were estimated to be 100, 45, and 40 kDa, respectively, by SDS-PAGE. The optimum pH and temperature of BGL A3 were 5.0 and 50°C, respectively, whereas the optimum pH and temperature of BGL A1 and BGL-A2 were identical (4...
April 12, 2018: Journal of Microbiology and Biotechnology
Jeong Yeon Seo, Ji Won Choi, Jae Yeon Lee, Young Shik Park, Yong Il Park
Although ginseng marc is a by-product obtained during manufacturing of various commercial ginseng products and has been routinely discarded as a waste, it still contains considerable amounts of potential bioactive compounds, including saponins and polysaccharides. Previously, we reported that ginseng oligosaccharides derived from ginseng marc polysaccharides by enzymatic hydrolysis exert immunostimulatory activities in macrophages and these activated macrophages are in turn able to inhibit the growth of skin melanoma cells by inducing apoptosis...
April 12, 2018: Journal of Microbiology and Biotechnology
Hwan Hee Lee, Hyosun Cho
Curcumin is known to possess various biological functions including anti-inflammatory, antioxidative, and anti-cancer activity. Natural killer (NK) cells are large lymphocytes that directly kill cancer cells. However, many aggressive cancers including breast cancer were reported to escape the successful killing of NK cells in a tumor microenvironment. In this study, we investigated the anti-cancer effect of curcumin in coculture of human breast carcinoma MDA-MB231 and NK (NK-92) cells. We found that curcumin had an immune8 stimulatory effect on NK-92 by increasing the surface expression of the CD16+ and CD56dim population of NK-92...
April 12, 2018: Journal of Microbiology and Biotechnology
Wei-Jie Wu, Byung-Young Ahn
Optimization of the culture medium to maximize menaquinone-7 (MK-7) production by Bacillus subtilis strain KCTC 12392BP in static culture was carried out using statistical experimental methods including one factor at a time, fractional factorial design, and response surface methodology (RSM). Maltose (carbon source), tryptone (nitrogen source), and glycerol (activator) were identified as the key medium components for MK-7 synthesis by a fraction factorial design, and were selected for statistical optimization by RSM...
April 12, 2018: Journal of Microbiology and Biotechnology
Mira Oh, Jae Woo Han, Chanhui Lee, Gyung Ja Choi, Hun Kim
In the course of screening for finding of microbes with nematicidal activity, we found that Enterobacter asburiae HK169 displayed promising nematicidal activity against root-knot nematode Meloidogyne incognita along with plant growth-promoting properties. Soil drenching of a culture of HK169 reduced gall formation by 66% while also increasing root and shoot weights by 251 and 160%, respectively, compared to an untreated control. The cell-free culture filtrate of the HK169 culture killed all juveniles of M. incognita within 48 h...
April 12, 2018: Journal of Microbiology and Biotechnology
Jin Wook Lee, Yoon Joong Kang, Hyun Kyung Choi, Young Geol Yoon
Coptis chinensis (CC) is widely used in Asian countries to treat inflammatory diseases. We investigated the anti-inflammatory activity of the aqueous fraction separated from CC extract and of berberine, its key bioactive component, in human keratinocytes and the possible molecular mechanisms underlying this. Treating HaCaT keratinocytic cells with heat-killed Propionibacterium acnes induced nitric oxide and proinflammatory cytokine [e.g., tumor necrosis factor-α, interleukin (IL)-1β, and IL-8] production and their mRNA expressions; these effects were suppressed by pretreatment with the aqueous fraction or berberine, which also suppressed the phosphorylation of ERK, JNK, and p38 kinases and the nuclear expression of the nuclear factor (NF)-κB p65 in P...
April 12, 2018: Journal of Microbiology and Biotechnology
Eun-Ha Kim, Hyeok-Il Kwon, Su-Jin Park, Young-Il Kim, Young-Jae Si, In-Won Lee, Se Mi Kim, Soo-In Kim, Dong-Ho Ahn, Young Ki Choi
As shown during the 2009 pandemic H1N1 [A(H1N1)pdm09] outbreak, egg-based influenza vaccine production technology is insufficient to meet global demand during an influenza pandemic. Therefore, there is a need to adapt cell culture-derived vaccine technology using suspended cell lines for more rapid and larger-scale vaccine production. In this study, we attempted to generate a high growth influenza vaccine strain in MDCK cells using an A/Puerto/8/1934 (H1N1) vaccine seed strain. Following 48 serial passages with four rounds of virus plaque purification in MDCK cells, we were able to select several MDCK-adapted plaques that could grow over 108 PFU/ml...
April 12, 2018: Journal of Microbiology and Biotechnology
Junho Lee, Areum Lee, Keumok Moon, Kyoung-Hwa Choi, Jaeho Cha
Previously, a cytosolic trehalase(TreH) from the hyperthermophilic archaeon Sulfolobusacidocaldarius was reported, however, a gene responsible for the trehalase activity was not identified. Two genes, saci_1816 and saci_1250,that encode the glycoside hydrolase family 15 type glucoamylase-like proteinsin S.acidocaldarius were targeted and expressed in Escherichia coli , and their abilities to hydrolyze trehalosewere examined. Recombinant Saci_1816 hydrolyzeda trehaloseexclusively without any help from acofactor...
April 12, 2018: Journal of Microbiology and Biotechnology
Wonho Choi, Min-Ho Yoon, Jung-Ho Park
Bacterial programmed cell death (PCD) is regulated by the toxin-antitoxin (TA) system. YhaV (toxin)and Pr1F (antitoxin) have been recently identified as a type II TA system in Escherichia coli . YhaV homologs have conserved active residues within the C terminus, and to characterize the function of this region, we purified native YhaV protein (without denaturing) and constructed YhaV proteins of varying lengths. Here, we report a new low-temperature method of purifying native YhaV, which is notable given the existing challenges of purifying this highly toxic protein...
April 12, 2018: Journal of Microbiology and Biotechnology
Insuk Sim, Keun-Tae Park, Gayeung Kwon, Jong-Ho Koh, Young-Hee Lim
Probiotics including Enterococcus faecium confer a health benefit on the host. An Enterococcus strain was isolated from healthy chicken cecum, identified as E. faeciumby 16S rDNA gene sequence analysis, and designatedas E. faecium L11.Toevaluate the potential of E. faecium L11 as a probiotics, the gastrointestinal tolerance, immunomodulatory activity, and lifespan extension properties of the strain were assayed. E.faecium L11 showed >66% and >62% survival in artificial gastric juices (0.3% pepsin, pH 2...
April 12, 2018: Journal of Microbiology and Biotechnology
Da-Eun Jeong, Younju So, Hayeon Lim, Seung-Hwan Park, Soo-Keun Choi
Bacillus strains produce various types of antibiotics, and random mutagenesis has traditionally been used to overproduce these natural metabolites. However, this method leads to the accumulation of unwanted mutations in the genome. Here, we used rationally designed a single nucleotide substitution in the degU gene to generate a B. subtilis strain displaying increased plipastatin production in a foreign DNA-free manner. The mutant strain (BS1028u) showed improved antifungal activity against Pythium ultimum ...
April 12, 2018: Journal of Microbiology and Biotechnology
Zhimin Ou, Jiahui Xu, Lihua Du, Lan Tang, Yangping Niu, Jian Cui
In this study, interface-assembled carbonyl reductase (IACR) is prepared and used in the synthesis of S-licarbazepine in a toluene/Tris-HCl biphasic system. The carbonyl reductase (CR) is conjugated with polystyrene to form a surfactant-like structure at the interface of the toluene/Tris-HCl biphasic system. The interface-assembled efficiency of IACR reached 83% when the CR (180 U/mg) and polystyrene concentration were 0.8 g/l and 3.75 g/l, respectively. The conversion reached 95.6% and the enantiometric excess of S-licarbazepine was 98...
April 6, 2018: Journal of Microbiology and Biotechnology
Hongyu Wen, Xin Yin, Zhenya Yuan, Xiuying Wang, Siting Su
Diarrhea is a global disease with the high morbidity and mortality rate in children. In this study, 25 fecal samples were collected from children under 5 years old. Seven samples have been taken from healthy children without diarrhea and marked as the healthy control group; eight samples have been sampled from the children with diarrhea caused by dyspepsia and defined as thenon-infectious group, and ten samples have been taken from children with diarrhea induced by intestinal infections and identified as the infectious group...
April 6, 2018: Journal of Microbiology and Biotechnology
Yu-Ru Zhao, Hong-Mei Li, Meilin Zhu, Jing Li, Tao Ma, Qiang Huo, Young-Soo Hong, Cheng-Zhu Wu
Heat shock protein 90 (Hsp90) is treated as a molecular therapeutic target for the prevention and treatment of cancer. Geldanamycin (GA) was the first identified natural Hsp90 inhibitor, but hepatotoxicity has limited its clinical application. Nevertheless, a new GA analog (WK-88-1) with the non-benzoquinone skeleton, obtained from the genetically engineered Streptomyces hygroscopicus , was found to haveanticancer activity against two human breast cancer cell lines.WK-88-1 produced concentration-dependent inhibition in cell proliferation, cell cycle arrest, and apoptosis inestrogen receptor (ER)-positive MCF-7 andER-negativeMDA-MB-231 cell lines...
April 6, 2018: Journal of Microbiology and Biotechnology
Qianhui Huang, Xing Han, Irum Mukhtar, Lingling Gao, Rongmei Huang, Liping Fu, Junjie Yan, Yongxin Tao, Bingzhi Chen, Baogui Xie
Expansins are cell wall proteins that mediate cell wall loosening and promote specific tissue and organ morphogenesis in plants and in some microorganisms. Unlike plant expansins, the biological functions of fungal expansin-like proteins haverarely been discussed. In the present study, an expansin-like protein encoding gene, fvexpl1 , was identified from F. velutipes by using local BLAST. It consisted of 5 exons with a total length 822bp. The deduced protein FVEXPL1contained 274 amino acids with predicted molecular weight and isoelectric point 28589 Da and pH4...
April 6, 2018: Journal of Microbiology and Biotechnology
Heo-Myung Jung, Yeon-Hee Kim
A copy number amplification system for yeast artificial chromosome (YAC) was combined with simultaneous overexpression of genes integrated intoa YAC. The chromosome VII (1,105 kb) was successfully split to 887 kb, 44 kb containing the element for copy number amplification, and 184 kb split-YAC. The 44 kb split-mini YAC was amplified maximum 9 fold, and the activity of the reporter enzymes integrated intothe split-mini YAC increasedabout 5 to 7 fold. These results demonstrate that the mini-YAC containing a targeted chromosome region can be readily amplified, and the specific genes in the mini-YAC could be overexpressed by increasing the copy number...
March 16, 2018: Journal of Microbiology and Biotechnology
Youngdeuk Lee, Eunyoung Jo, Yeon-Ju Lee, Sachithra Amarin Hettiarachchi, Gun-Hoo Park, Su-Jin Lee, Soo-Jin Heo, Do-Hyung Kang, Chulhong Oh
The agarase gene gaa16a was identified from a draft genome sequence of Gilvimarinus agarilyticus JEA5, an agar-utilizing marine bacterium. Recently, three agarase-producing bacteria, G. chinensis , G. polysaccharolyticus , and G. agarilyticus , in the genus Gilvimarinus were reported. However, there have been no reports of the molecular characteristics and biochemical properties of these agarases. In this study, we analyzed the molecular characteristics and biochemical properties of agarases in Gilvimarinus ...
March 16, 2018: Journal of Microbiology and Biotechnology
Sasikumar Arunachalam Palaniyandi, Karthiyaini Damodharan, Joo-Won Suh, Seung Hwan Yang
The present study was focused on the production, characterization and in vitro prebiotic evaluation of exopolysaccharide (EPS) from Bacillus sonorensis MJM60135isolated from Ganjang (fermented soy sauce). StrainMJM60135 showed the highest production(8.4±0.8 g/L) of EPS compared to other isolates that were screened for EPS production based on ropy culture morphology. Further, MJM60135 was cultured in 5L medium and EPS was extracted by ethanol precipitation. Emulsification activity of EPS was higher in toluene than o -xylene...
March 16, 2018: Journal of Microbiology and Biotechnology
Dayoung Kang, Zhipeng Li, Geun Eog Ji
Ginseng and probiotics have anti-obese effects in mice fed a high-fat diet(HFD). Absorption of ginsenoside and colonization of probiotics occur in the intestine. In this study, the mixture of fermented ginseng and two probiotics, Bifidobacterium longum BORI and Lactobacillus paracasei CH88, was administered to HFD fed mice for 9 weeks. The mixture significantly suppressed weight gain ( p < 0.05, n = 8) and lipid deposition in the liver and adipose tissues as well as increased the mice's food intake. The adipocyte size of the adipose tissue was significantly decreased in the mixture group, especially when 0...
March 16, 2018: Journal of Microbiology and Biotechnology
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