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Journal of Microbiology and Biotechnology

Young Woong Lee, Young Eun Hwang, Ju Young Lee, Jung-Hoon Sohn, Bong Hyun Sung, Sun Chang Kim
RNA interference provides an effective tool for developing antitumor therapies. Cell-penetrating peptides (CPPs) are delivery vectors widely used to efficiently transport small-interfering RNA (siRNA) to intracellular targets. In this study, we investigated the efficacy of the cancer-specific CPP carrier BR2 to specifically transport siRNA to cancer-target cells. Our results showed that BR2 formed a complex with anti-vascular endothelial growth factor siRNA (siVEGF) that exhibited the appropriate size and surface charge for in vivo treatment...
January 11, 2018: Journal of Microbiology and Biotechnology
Tae-Eui Lee, Kyung-Chul Shin, Deok-Kun Oh
D-Allose is a potential medical sugar because it has anti-cancer, anti-hypertensive, anti-inflammatory, anti-oxidative, and immunosuppressant activities. Allose production from fructose as a cheap substrate was performed byone-pot reaction using Flavonifractor plautiiD-allulose 3-epimerase (FP-DAE) and Clostridium thermocellumribose 5-phosphate isomerase (CT-RPI). The optimal reaction conditions for allose production were pH 7.5, 60°C,0.1 g/l FP-DAE, 12 g/l CT-RPI, and 600 g/l fructose in the presence of 1 mM Co²⁺...
January 11, 2018: Journal of Microbiology and Biotechnology
Hee-Young Ahn, Young-Moo Choo, Young-Su Cho
We have previously found that mycelia culture broth of eight kinds of traditional herbal extracts fermented with Phellinus linteus (previously named as 8-HsPLCB) not only inhibited melanin and tyrosinase activity, but also reduced the contents of melanogenesis-related proteins including tyrosinase and microphthalmia-associated transcription factor (MITF) in 3- isobutyl-1-methylxanthine (IBMX)-stimulated B16F0 melanoma cells. For a further study, the effect of 8-HsPLCB against skin pigmentation in brown guinea pigs of ultraviolet B (UVB)-induced hyperpigmentation was investigated...
January 11, 2018: Journal of Microbiology and Biotechnology
In Young Choi, JooHyeon Park, Kwang-Pyo Kim, Jun-Hyun Oh, Mi-Kyung Park
This study investigated the feasibility of the lytic, tailed Bacillus cereus-specific phage for use in a ferromagnetoelastic (FME) biosensor as a novel recognition element. The phage was immobilized at various concentrations through a direct adsorption or a combination of 11-MUA and EDC/NHS. The effects of time and temperature on its lytic properties were investigated through the exposure of B. cereus (4 and 8 logCFU/ml) to the phage (8 logPFU/ml) for various incubation periods at 22°C and at various temperatures for 30 and 60 min...
January 11, 2018: Journal of Microbiology and Biotechnology
Ji-Sun Kim, Ji-Ho Jeong, Yongae Kim
We have previously derived a novel antimicrobial peptide, LPcin-YK3(YK3), based on lactophoricin and have successfully studied and reported on the relationship between structure and function.In this study, antimicrobial peptides with improved antimicrobial activity, less cytotoxicity and shorter length were devised and characterized based on YK3, and named YK5, YK8, YK11.The peptide design was based on a variety of knowledge, and a total of nine analog peptides consist of one to three amino acid substitutions and C-terminal deletions...
January 11, 2018: Journal of Microbiology and Biotechnology
Bong-Soo Kim, Dong-Hun Han, Ho Lee, Bumjo Oh
Salivary microbiota alterations can correlate with dental caries development in children,and mechanisms mediating this association need to be studied in further detail. Our study explored salivary microbiota shifts in children and their association with the incidence of dental caries with dentin involvement. Salivary samples were collected from children with caries and their subsequently matched caries-free controls before and after caries development. The microbiota was analyzed by 16S rRNA gene-based high-throughput sequencing...
January 11, 2018: Journal of Microbiology and Biotechnology
Yesol Bak, Hui-Joo Jang, Ji-Hye Seo, Su-Hyun No, Jung-Il Chae, Jintae Hong, Do-Young Yoon
Benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon, is a principal component of cigarette smoke. B[a]P can cause lung carcinogenesis and plays a key role in lung cancer progression. The role of B[a]P has been reported in lung cancer, but its effects on lung cancer stem cells have not been investigated. Emerging evidence indicates that cancer stem cells (CSCs) are associated with carcinogenesis, tumor initiation, relapse, and metastasis. Therefore, targeting CSCs to defeat cancer is a challenging issue in the clinic...
January 11, 2018: Journal of Microbiology and Biotechnology
Heung-Shick Lee, Younhee Kim
Candida albicans infections are problematic to treat often due to antifungal resistance, since most infections of the pathogen are associated with biofilms. The abilities of C. albicans to switch from budding yeast to filamentous hyphae, and adhere to host cells or various surfaces support biofilm formation. We previously reported that the ethanol extract from Paeonia lactiflora not only inhibits cell wall synthesis but also causes depolarization and permeabilization of cell membrane in C. albicans. In this study, we found that the P...
January 11, 2018: Journal of Microbiology and Biotechnology
Su-Jin Lee, Woo-Seok Choi, Gun-Hoo Park, Tae-Ho Kim, Chulhong Oh, Soo-Jin Heo, Do-Hyung Kang
Microalgae hold promise as a renewable energy source for the production of biofuel, as they can convert light energy into chemical energy through photosynthesis. However, cost-efficient harvest of microalgae remains a major challenge to commercial-scale algal biofuel production. We firstly investigated the potential of electrolytic water as a flocculantfor harvesting Tetraselmis sp.Alkaline electrolytic water (AEW) is produced at cathode through water electrolysis.It contains mineral ions such as Na⁺, K⁺, Ca²⁺ and Mg²⁺ that can act as flocculant...
January 11, 2018: Journal of Microbiology and Biotechnology
Sujan Piya, Tae-Hyoung Kim
Lipopolysaccharide (LPS), a component of the cell wall of gram-negative bacteria, elicits the secretion of cytokines, such as interferons, that stimulate the host defense system. Previously, we demonstrated that interferons induce interferon regulatory factors (IRFs) 1, 3, and 7 that regulate transcription of Noxa and alter expression profiles of Bcl-2 family proteins in tumors. However, the immediate consequences of LPS stimulation on Noxa and BH3 expression in tumor cells remain uncharacterized. Here, we determined that LPS induced Noxa expression in CT26 cells...
January 11, 2018: Journal of Microbiology and Biotechnology
Hui Yang, Kwang-Hoon Oh, Hyun Jin Kim, Young Ho Cho, Yung Choon Yoo
It is well known that Korean red ginseng has various biological activities. However, there is little knowledge about antiviral activity of Korean red ginseng and its ginsenosides. In this study, we addressed whether oral administration of ginsenoside-Rb2 and -Rg3 were able to protect against rotavirus (RV) infection. Protective effect of ginsenosides against RV infection was examined using an in vivo experiment model in which newborn mice (10-day-old) were inoculated perorally (p.o.) with 1.5X106 plaque-forming units (PFU)/mouse of RV strain SA11...
January 11, 2018: Journal of Microbiology and Biotechnology
Youngdae Yoon, Yerin Kang, Woonwoo Lee, Sunghoon Kim, Ki-Cheol Oh, Bong-Gyu Kim
In Escherichia coli, the transcription of genes related to metal homeostasis is activated by the presence of target metals. The promoter regions of those genes can be fused with reporter genes to generate whole-cell bioreporters (WCBs); these organisms sense the presence of target metals through reporter gene expression. However, the limited number of available promoters for sensing domains restricts the number of WCB targets. In this study, we have demonstrated an alternative method to generate novel WCBs, based on the notion that since the sensing mechanisms of WCBs are related to metal transportation systems, their properties can be modulated by disrupting metal homeostasis...
December 8, 2017: Journal of Microbiology and Biotechnology
Ramesh Prasad Pandey, Luan Luong Chu, Tae-Su Kim, Jae Kyung Sohng
Single vessel multi-enzyme UDP-α-D-glucose recycling system was coupled with a forward glucosylation reaction to produce glucose moiety conjugated novel derivatives of different tetracycline antibiotic analogues. Among five tetracycline analogues used for the reaction, four molecules (chlorotetracycline, doxytetracycline, meclotetracycline, and minotetracycline) were accepted by a glycosyltransferase enzyme, YjiC, from Bacillus licheniformis to produce glucoside derivatives. However, the enzyme was unable to conjugate sugar unit to rolitetracycline...
December 8, 2017: Journal of Microbiology and Biotechnology
Mi-Ae Han, Seong-Joo Hong, Z-Hun Kim, Byung-Kwan Cho, Hookeun Lee, Hyung-Kyoon Choi, Choul-Gyun Lee
Lipids in microalgae are energy-rich compounds and considered as an attractive feedstock for biodiesel production. To redirect carbon flux from competing pathways to the fatty acid synthesis pathway of Tetraselmis sp., we used three types of chemical inhibitors, which can block the starch synthesis pathway or photorespiration, under nitrogen-sufficient and nitrogen-deficient conditions. The starch synthesis pathway in chloroplasts and the cytosol can be inhibited by 3-(3,4-dichlorophenyl)-1,1-dimethylurea and 1,2-cyclohexanediamine tetra acetic acid, respectively...
December 8, 2017: Journal of Microbiology and Biotechnology
Chaiwat Monmai, Seok Hyeon Go, Il-Sik Shin, SangGuan You, Hyungjae Lee, SeokBeom Kang, Woo Jung Park
Asterias amurensis is a marine organism that causes damage to the fishing industry worldwide; however, it has been considered a promising source of functional components. The present study aimed to investigate the immune-enhancing effects of fatty acids from three organs of A. amurensis on murine macrophages (RAW 264.7 cells). A. amurensis fatty acids boosted production of immune-associated factors such as nitric oxide (NO) and prostaglandin E2 in RAW 264.7 cells. A. amurensis fatty acids also enhanced expression of critical immune-associated genes, including iNOS, TNF-α, IL-1β, and IL-6, as well as COX-2...
December 8, 2017: Journal of Microbiology and Biotechnology
Haebom Kim, Changhee Kim, Do Un Kim, Hee Chul Chung, Jae-Kwan Hwang
Periodontitis, an infective disease caused by oral pathogens and the intrinsic aging process, results in the destruction of periodontal tissues and alveolar bone loss. This study investigated whether Boesenbergia pandurata extract (BPE) standardized with panduratin A exerted anti-periodontitis effects using an aging model representative of naturally occurring periodontitis. In aged rats, the oral administration of BPE (200 mg/kg/day) for 8 weeks significantly reduced the mRNA and protein expression of interleukin (IL)-1β, nuclear factor-kappaB (NF-κB), matrix metalloproteinase (MMP)-2, and MMP-8 in gingival tissues (p < 0...
December 8, 2017: Journal of Microbiology and Biotechnology
Hyun-do Kim, Su-Mi Kim, Jong-Il Choi
In this study, a 107 kDa protease from psychrophilic Janthinobacterium lividum PAMC 26541 was purified by anion exchange chromatography. The specific activity of the purified protease was 264 U/mg, and the overall yield was 12.5%. The J. lividum PAMC 25641 protease showed optimal activity at pH 7.0-7.5 and 40°C. Protease activity was inhibited by PMSF, but not by DTT. Based on the N-terminal sequence of the purified protease, the gene encoding the cold-adapted protease from J. lividum PAMC 25641 was cloned into the pET-28a(+) vector and heterologously expressed in Escherichia coli BL21(DE3) as an intracellular soluble protein...
December 8, 2017: Journal of Microbiology and Biotechnology
In Yung Sunwoo, Trung Hau Nguyen, Pailin Sukwong, Gwi-Teak Jeong, Sung-Koo Kim
The waste seaweed from Gwangalli beach, Busan, Korea was utilized as biomass for ethanol production. Sagassum fulvellum (brown seaweed, Mojaban in Korean name) comprised 72% of the biomass. The optimal hyper thermal acid hydrolysis conditions were obtained as 8% slurry contents, 138 mM sulfuric acid and 160 °C of treatment temperature for 10 min with a low content of inhibitory compounds. To obtain more monosaccharide, enzymatic saccharification was carried out with Viscozyme L for 48 h. After pretreatment, 34 g/L of monosaccharides were obtained...
December 8, 2017: Journal of Microbiology and Biotechnology
Yong-Woo Shin, Deok Bum Park, Myung-Min Choi, Jeong-Hoon Chun, Baik-Lin Seong, Gi-Eun Rhie
Molecular imaging is a powerful method for tracking various infectious disease causing pathogens in host organisms. Currently, a dual molecular imaging method which can provide temporal and spatial information in infected hosts at organism, organ, tissue and cellular levels simultaneously has not been reported for Burkholderia pseudomallei, a high-risk pathogen that causes melioidosis. In this study, we have established an experiment method that provides spatio-temporal information in infected hosts using luminescent and fluorescent dual-labeled B...
December 8, 2017: Journal of Microbiology and Biotechnology
Oh Joo Kweon, Yong Kwan Lim, Hye Ryoun Kim, Tae-Hyoung Kim, Mi-Kyung Lee
Our aim was to determine the detection rate of respiratory viruses (RVs) in feces of patients with acute viral respiratory infection (AVRI) and the detection rate of diarrheal viruses (DVs) in nasopharyngeal samples from patients with acute viral gastroenteritis. The relationships between the presence of fecal RVs or nasopharyngeal DVs and their impacts on the clinical severity were also investigated. Total 144 fecal specimens collected from AVRI patients and 95 nasopharyngeal specimens collected from acute viral gastroenteritis patients...
December 8, 2017: Journal of Microbiology and Biotechnology
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