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Journal of Microbiology and Biotechnology

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https://www.readbyqxmd.com/read/30199924/hepatitis-e-virus-methyltransferase-inhibits-type-i-interferon-induction-by-targeting-rig-i
#1
Sangmin Kang, Changsun Choi, Insoo Choi, Kwi-Nam Han, Seong Woon Roh, Jongsun Choi, Joseph Kwon, Mi-Kyung Park, Seong-Jun Kim, Jinjong Myoung
The type I interferons (IFNs) play a vital role in activation of innate immunity in response to viral infection. Accordingly, viruses have evolved to employ various survival strategies to evade innate immune responses induced by type I IFNs. For example, HEV encoded papain-like cysteine protease (PCP) has been shown to inhibit IFN activation signaling by suppressing K63-linked de-ubiquitination of retinoic acid-inducible gene I (RIG-I) and TANK-binding kinase 1 (TBK1), thus effectively inhibiting down-stream activation of IFN signaling...
September 10, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30199923/growth-performance-and-post-weaning-diarrhoea-in-piglets-fed-a-diet-supplemented-with-probiotic-complexes
#2
Xuhong Lu, Ming Zhang, Liang Zhao, Keshan Ge, Zongyi Wang, Luo Jun, Fazheng Ren
Weaning stress can affect the growth performance and intestinal health of piglets. Dietary alternatives to antibiotics, such as dietary probiotics, especially those containing multiple microbial species, are a preventive strategy for effectively controlling post-weaning diarrhoea. In this study, we investigated forty-eight crossbred piglets in three treatment groups for 21 days: the control and experimental group supplemented with Enterococcus faecium DSM 7134, Bacillus subtilis AS1.836 plus Saccharomyces cerevisiae ATCC 28338 (EBS) or Lactobacillus paracasei L9 CGMCC No...
September 10, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30196596/complete-genome-of-bacillussubtilis-subsp-subtilis-kctc-3135-t-and-variation-in-cell-wall-genes-of-b-subtilis-strains
#3
Seonjoo Ahn, Sangmi Jun, Hyun-Joo Ro, Ju Han Kim, Seil Kim
The type strain Bacillus subtilis subsp. subtilis KCTC 3135T was deeply sequenced and annotated, replacing a previous draft genome in this study. The tar and tag genes were involved in synthesizing wall teichoic acids (WTAs), and these genes and their products were previously regarded as the distinguishing difference between B. s. subtilis and B. s. spizizenii . However, a comparative genomic analysis of B. subtilis spp. revealed that both B. s. subtilis and B. s. spizizenii had various types of cell walls...
September 3, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30196595/high-level-production-of-high-purity-human-and-murine-recombinant-prion-proteins-functionally-compatible-to-in-vitro-seeding-assay
#4
Hae-Gwang Hwang, Dae-Hwan Kim, Jeongmin Lee, Youngwon Mo, Se-Hoon Lee, Yongjin Lee, Jae Wook Hyeon, Sol Moe Lee, Yong-Pil Cheon, Eun-Kyoung Choi, Su Yeon Kim, Yeong Seon Lee, Young-Jin Son, Chongsuk Ryou
Recombinant (rec) prion protein (PrP) is an extremely useful resource to study protein misfolding and subsequent protein aggregation events. Here, we report mass production of high-purity rec-polypeptide encoding the C-terminal globular domain of PrP; (90-230) for human and (89-231) for murine PrP. These proteins were expressed as His-tagged fusion proteins in E. coli . cultured by ahigh cell density aerobic fermentation method. RecPrPs recovered from inclusion bodies were slowly refolded under reducing conditions...
September 3, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30196594/proteolytic-system-of-streptococcus-thermophilus-a-review
#5
Gabriela Mariana Rodríguez-Serrano, Jose Mariano Garcia-Garibay, Alma Elizabeth Cruz-Guerrero, Lorena Del Carmen Gomez-Ruiz, Alexis Ayala-Nino, Araceli Castaneda-Ovando, Luis Guillermo Gonzalez-Olivares
The growth of lactic acid bacteria (LAB) generates a high number of metabolites related to aromas and flavors in fermented dairy foods. These microbial proteases are involved in protein hydrolysis that produce necessary peptides for their growth,releasing different molecules of interest like bioactive peptides, during their activity. Each genus in particular has its proteolytic system to hydrolyze the necessary proteins to meet its requirements. This review aims at highlighting the differences between the proteolytic system of Streptococcus thermophilus and other lactic acid bacteria ( Lactococcus and Lactobacillus ) since it is a microorganism that is frequently used in combination with other LAB in the elaboration of fermented dairy products...
September 3, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30196593/-fusarium-mangiferae-as-new-cell-factories-for-producing-silver-nanoparticles
#6
Haider Mousa Hamzah, Reyam Faris Salah, Mohammed Nadheer Maroof
Finding a safe and broad spectrum medication is a goal of scientists, pharmacists, and physicians, but developing and fabricating the right medicine can be challenging. The current study describes the formation of silver nanoparticles (AgNPs) by Fusarium mangiferae . It involves the antibiofilm activity of the nanoparticles against Staphylococcus aureus . It also involves cytotoxic effect against mammalian cell lines. Well-dispersed nanoparticles are formed by F. mangiferae . The sizes of the nanoparticles were found to range from 25 to 52 nm, and UV-Vis scan showed absorption around 416-420 nm...
September 3, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30196592/transcriptional-response-and-enhanced-of-intestinal-adhesion-ability-of-lactobacillus-rhamnosus-gg-after-acid-stress
#7
Miseon Bang, ChengChung Yong, Hyeok-Jin Ko, In-Geol Choi, Sejong Oh
Lactobacillus rhamnosus GG (LGG) is a probiotic commonly used in fermented dairy products. In this study, RNA-sequencing was performed to unravel the effects of acid stress on LGG.The transcriptomics data revealed that the exposure of LGG to acid at pH 4.5 (resembling the final pH of fermented dairy products) for 1 h or 24 h provoked a stringent-type transcriptomic response wherein stress response- and glycolysis-related genes were upregulated, whereas genes involved in gluconeogenesis, amino acids metabolisms, and nucleotide metabolism were suppressed...
September 3, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30196591/inhibitory-effect-of-standardized-curcuma-xanthorrhiza-supercritical-extract-on-lps-induced-periodontitis-in-rats
#8
Kyo Eun Kook, Changhee Kim, Wonku Kang, Jae-Kwan Hwang
Periodontitis, which is a severe inflammatory disease by endotoxins secreted from oral pathogens, destructs gingival tissue and alveolar bone. Curcuma xanthorrhiza , commonly called Java tumeric, has been shown to possess anti-bacterial and anti-inflammatory activities. The present study evaluated the inhibitory effect of C. xanthorrhiza supercritical extract (CXS) standardized with xanthorrhizol on lipopolysaccharide (LPS)-induced periodontitis in animal model. LPS was topically injected to the periodontium of Sprague-Dawley rats to induce periodontitis and CXS (30 and 100 mg·kg-1 ·day-1 ) was orally administered after day 12...
September 3, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30196590/emodin-successfully-inhibited-invasion-of-brucella-abortus-via-modulating-adherence-microtubule-dynamics-and-erk-signaling-pathway-in-raw-264-7-cells
#9
Tran Xuan Ngoc Huy, Alisha Wehdnesday Bernardo Reyes, Huynh Tan Hop, Lauren Togonon Arayan, Vu Hai Son, Wongi Min, Hu Jang Lee, Suk Kim
The aim of this work is to investigate the protective efficacy of emodin, an active naturally occurring anthraquinone derivative of several traditional Chinese herbs, against Brucella abortus infection in macrophages. Brucella were incubated with different concentrations of emodin and showed that bacterial survival rates were markedly reduced in a dose-dependent manner at increasing incubation time points. Through bacterial infection assay, the highest non-cytotoxic concentration of emodin demonstrated attenuated invasion of Brucella into macrophages, however it did not inhibit the growth of these pathogens within the host cells...
September 3, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30178651/fermentation-mediated-enhancement-of-ginseng-s-anti-allergic-activity-against-ige-mediated-passive-cutaneous-anaphylaxis-in-vivo-and-in-vitro
#10
Seon-Weon Hwang, Xiao Sun, Jun-Hyuk Han, Tae-Yeon Kim, Sushruta Koppula, Tae-Bong Kang, Jae-Kwan Hwang, Kwang Ho Lee
Ginseng (the root of Panax ginseng Meyer) fermented by Lactobacillus plantarum has been found to attenuate allergic responses in in vitro and in vivo experimental models.Ginseng has been reported to also possess various biological functions including anti-inflammatory activity. The present study was aimed at comparing the anti-allergic effect of ginseng and fermented ginseng extracts on IgE-mediated passive cutaneous anaphylaxis in vitro in a murine cell line and in vivo in mice.Fermented ginseng extract (FPG) showed higher inhibitory effect against in vitro and in vivo allergic responses when compared with ginseng extract (PG)...
August 24, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30178650/tio2-nanoparticles-from-bakers-yeast-a-potent-antimicrobial
#11
Mudara Peiris, Chinthika Gunasekara, Pradeep Jayaweera, Neluka Fernando
Titanium dioxide (TiO2 ) has wide applications in food industry, cosmetics, pharmaceuticals and manufacturing industries due to its' many properties such as photocatalytic activity and stability. In this study, biosynthesis of TiO2 nanoparticles was achieved by using Baker's yeast. TiO2 nanoparticles were characterized by X-ray Diffraction (XRD), UV-Visible spectroscopy, Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Energy Dispersive X-ray analysis (EDX) studies. The XRD pattern confirmed the formation of pure anatase TiO2 nanoparticles...
August 24, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30178649/novel-approach-into-efficient-antifungal-drug-action
#12
Heejeong Lee, Dong Gun Lee
The emergence of multidrug-resistant microorganisms, as well as fungal infectious diseases that further threaten health, especially in immunodeficient populations, is a major global problem. The development of new antifungal agents in clinical trials is inferior to the incidence of drug resistance, and the available antifungal agents are restricted. Their mechanisms aim at certain characteristics of the fungus in order to avoid biological similarities with the host.The synthesis of cell wall and ergosterol is mainly targeted for clinical use...
August 24, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30178648/saccharification-of-brown-macroalgae-using-an-arsenal-of-recombinant-alginate-lyases-potential-application-in-the-biorefinery-process
#13
Javier A Gimpel, María Cristina Ravanal, Oriana Salazar, Maria Elena Lienqueo
Alginate lyases (endo and exo-lyases) are required for the degradation of alginate into its constituting monomers. Efficient bioethanol production and extraction of bioactives from brown algae requires intensive use of these enzymes. Nonetheless, there are few commercial alginate lyase preparations, and their costs make them unsuitable for large scale experiments. A recombinant expression protocol has been developed in this study for producingseven endo-lyases and three exo-lyases as soluble and highly active preparations...
August 24, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30178647/laboratory-investigation-of-trends-in-bacterial-pneumonia-in-cheonan-korea-from-january-2008-to-september-2017
#14
Young-Sam Yook, Jae-Sik Jeon, Ji On Park, Jae Kyung Kim
BACKGROUND: Bacterial pneumonia is one of the most common causes of mortality in Korea. In 2016, the mortality rate from pneumonia was 16,476 deaths per 100,000, which was an 11% increase from the previous year. The aim of our study was to determine the distribution of the bacterial pathogens causing respiratory symptoms in different age groups over a 10-year period. MATERIALS AND METHODS: Between January 2008 and September 2017, 1,861 specimens from 1,664 patients admitted to DankookUniversity Hospital with respiratory symptoms were examined...
August 24, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30178646/genes-related-to-intracellular-survival-of-brucella-abortus-in-thp-1-macrophage-cells
#15
Soojin Shim, Young Bin Im, Myunghwan Jung, Woo Bin Park, Han Sang Yoo
Brucella abortus can survive and replicate within host macrophages, and great efforts have been made to demonstrate the genes involved in pathogenicity such as internalization in Brucella research. Therefore, intracellular responses were compared between THP-1 macrophage cells stimulated with B. abortus wild-type and four mutants (C1, C10, C27, and C32) using microarray to demonstrate the role of genes related to intracellular survival and replication. These mutants were generated by deleting genes encoding BAB_RS13225 (4-hydrobenzoate 3-monooxygenase, PHBH), BAB_RS00455 (heme exporter protein cytochrome C, CcmC), BAB_RS03675 (exopolyphosphatase, PPX), and BAB_RS13225 (peptidase M24)...
August 24, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30178645/anti-inflammatory-effect-of-asterias-amurensis-fatty-acids-through-nf-%C3%AE%C2%BAb-and-mapk-pathways-against-lps-stimulated-raw264-7-cells
#16
Chaiwat Monmai, Seok Hyeon Go, Il-Shik Shin, SangGuan You, Dae-Ok Kim, SeokBeom Kang, Woo Jung Park
Asterias amurensis is a marine organism that damages the fishing industry, but also is a potential source of functional materials. The present study was conducted to analyze the profiles of fatty acids extracted from A. amurensis tissues and their anti-inflammatory effects on RAW264.7 macrophage cells. In different tissues, the component ratios of saturated fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids differed; particularly,polyunsaturated fatty acids such as dihomo-gamma linolenic acid (20:3n-6) and eicosapentaenoic acid (20:5n-3) were considerably different...
August 24, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30178644/optimized-expression-purification-and-rapid-detection-of-recombinant-influenza-nucleoproteins-expressed-in-sf9-insect-cells
#17
Sung-Jin Yoon, Young-Jun Park, Hyun Ju Kim, Jinwoo Jang, Sang Jun Lee, Sunwoo Koo, Moo-Seung Lee
Accurate and rapid diagnosis of influenza infection is essential to enable early antiviral treatment and reduce the mortality associated with seasonal and epidemic infections.Immunochromatography is one of the most common methods used for the diagnosis of seasonal human influenza; however, it is less effective for the diagnosis of pandemic influenza virus.Currently, rapid diagnosis kits for pandemic influenza virus rely on the detection of nucleoprotein (NP) or hemagglutinin (HA). NP detection shows higher specificity and is more sensitive than HA detection...
August 24, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30178643/adverse-effect-of-the-methanotroph-methylocystis-sp-m6-on-the-non-methylotroph-microbacterium-sp-nm2
#18
So-Yeon Jeong, Kyung-Suk Cho, Tae Gwan Kim
Several non-methylotrophic bacteria have been reported to improve the growth and activity of methanotrophs; however, their interactions remain to be elucidated. We investigated theinteraction between Methylocystis sp. M6 and Microbacterium sp. NM2.A batch co-culture experiment showed that NM2 markedly increased the biomass and methane removal of M6. qRT-PCR analysis revealed that NM2 enhanced both thegrowth and methane-monooxygenase gene expression of M6.A fed-batch experiment showed that co-culture was more efficient in removing methane than M6 alone (28...
August 24, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30178642/metabolic-engineering-of-deinococcus-radiodurans-for-the-production-of-phytoene
#19
Sun-Wook Jeong, Chang Keun Kang, Yong Jun Choi
A metabolically engineered Deinococcus radiodurans R1 strain capable of producing phytoene, a colorless C40 carotenoid and a promising antioxidant, was developed. First, to make a base strain, the crtI gene encoding phytoene desaturase was deleted to block the conversion of phytoene to other carotenoids such as lycopene and γ-carotene. This engineered strain produced 0.413 ± 0.023 mg/l of phytoene from 10 g/l of fructose. Further enhanced production of phytoene up to 4.46 ± 0.19 mg/l was achieved by overexpressing the crtB gene encoding phytoene synthase and the dxs genes encoding 1-deoxy-D-xylulose-5-phosphate synthase gene, and by deleting the crtD gene...
August 24, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30176714/the-positive-charge-of-arginine-residues-on-histone-h4-tail-is-required-for-the-maintenance-of-mating-type-in-saccharomyces-cerevisiae
#20
Soojin Yeom, Junsoo Oh, Eun-Jin Lee, Jung-Shin Lee
The transcriptional gene silencing is regulated by the chromatin structure changed by various factors including histones. Saccharomyces cerevisiae contains transcriptionally silenced regions such as telomeric regions and hidden mating (HM) loci. The positively-charged amino acids on the histone H4 tail were reported to be critical for the telomeric silencing in yeast, by interacting Dot1, a specific methyltransferase for the 79th lysine on histone H3. However, Dot1 did not affect gene silencing within HM loci, but whether the positively-charged amino acids on the H4 tail affects HM silencing has not been defined...
August 10, 2018: Journal of Microbiology and Biotechnology
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