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Journal of Microbiology and Biotechnology

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https://www.readbyqxmd.com/read/28423891/effective-thermal-inactivation-of-the-spores-of-bacillus-cereus-biofilms-using-microwave
#1
Hyong Seok Park, Jungwoo Yang, Hee Jung Choi, Kyoung Heon Kim
Microwave sterilization was performed to inactivate the spores of biofilms of Bacillus cereus involved in foodborne illness. The sterilization conditions, such as the amount of water biofilms were immersed in, temperature, and treatment time, were optimized after 15 runs based on the Box-Behnken design. The sterilization conditions, such as the amount of water and the operating temperature and treatment time were optimized using statistical analysis based on 15 runs experimental results designed by the Box- Behnken method...
April 20, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28376612/marine-algicolous-endophytic-fungi-a-promising-drug-resource-of-the-era
#2
REVIEW
Manomi Sarasan, Jayesh Puthumana, Neema Job, Jeonghoon Han, Jae-Seong Lee, Rosamma Philip
Endophytic fungi have currently been acknowledged, as the most promising source of bioactive compounds for drug discovery and considerable progress has been made in exploring their diversity, species richness, and bioprospecting. Fungal endophytes from unique environmental settings offer a pool of potentially useful medicinal entities. Due to the constant stresses imposed on macroalgae by marine environment, it is believed that algae and their associated endophytic symbionts represent a good source of structurally diverse bioactive secondary metabolites...
April 5, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28376611/rhus-verniciflua-stokes-extract-and-its-flavonoids-protect-pc-12-cells-against-h%C3%A2-o%C3%A2-induced-cytotoxicity
#3
Tae Gyu Nam, Bong Han Lee, Hyo-Kyoung Choi, Ahmad Rois Mansur, Sang Gil Lee, Dae-Ok Kim
Rhus verniciflua Stokes (RVS), an herbal medicine found in East Asia, was extracted and further fractionated to investigate its antioxidant capacity and neuroprotective effects. The RVS ethyl acetate (EtOAc) fraction had the highest level of total phenolics and antioxidant capacity among all solvent fractions tested. Pretreatment of PC-12 cells with the EtOAc fraction effectively attenuated H2O2-induced oxidative damage. Furthermore, the EtOAc fraction significantly attenuated caspase-3 activity, resulting in inhibition of H2O2-induced apoptosis...
April 5, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28376610/purification-and-characterization-of-the-laccase-involved-in-dye-decolorization-by-the-white-rot-fungus-marasmius-scorodonius
#4
Sung-Jong Jeon, Su-Jin Lim
Marasmius scorodonius secretes an extracellular laccase in potato dextrose broth, and this enzyme was purified up to 206-fold using (NH4)2SO4 precipitation and a Hi-trap Q Sepharose column. The molecular weight of the purified laccase was estimated to be ~67 kDa by SDS-PAGE. The UV/vis spectrum of the enzyme is nontypical for laccases, and metal content analysis revealed that the enzyme contains 1 mole of Fe and Zn and 2 moles of Cu per mole of protein. The optimal pH for the enzymatic activity was 3.4, 4.0 and 4...
April 5, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28372038/highly-time-resolved-metabolic-reprogramming-toward-differential-levels-of-phosphate-in-chlamydomonas-reinhardtii
#5
Cheol-Ho Jang, Gayeon Lee, Yong-Cheol Park, Kyoung Heon Kim, Do Yup Lee
Understanding phosphorus metabolism in photosynthetic organisms is important in that it is closely associated with enhanced crop productivity and pollution management for natural ecosystems (e.g. algal blooming). Accordingly, we exploited highly time-resolved metabolic responses to different levels of phosphate deprivation in C. reinhardtii, a photosynthetic model organism. We conducted non-targeted primary metabolite profiling using gas-chromatography time-of-flight mass spectrometric analysis. Primarily, we systematically identified main contributors to degree-wise responses corresponding to the deprivation levels of phosphate...
April 3, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28372037/construction-of-methanol-sensing-escherichia-coli-by-the-introduction-of-paracoccus-denitrificans-mxay-based-chimeric-two-component-system
#6
Irisappan Ganesh, Selvamani Vidhya, Gyeong Tae Eom, Soon Ho Hong
Escherichia coli, which does not have methanol sensing apparatus, was engineered to sense methanol by employing chimeric two-component system (TCS) strategy. A chimeric MxaY/EnvZ (MxaYZ) TCS was constructed by fusing the Paracoccus denitrificans MxaY with the E. coli EnvZ. Real-time quantitative PCR analysis and GFP-based fluorescence analysis showed that the ompC expression level and the fluorescence were increased as methanol concentration increased up to 0.01 %, respectively. These results suggested that E...
April 3, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28372036/bioethanol-production-from-hydrodictyon-reticulatum-by-fed-batch-fermentation-using-saccharomyces-cerevisiae-kctc7017
#7
Seul Ki Kim, Cuong Mai Nguyen, Eun Hye Ko, In-Chul Kim, Jin-Seog Kim, Jin-Cheol Kim
The aim of this study was to develop a potential process for bioethanol production from Hydrodictyon reticulatum (HR), a filamentous freshwater alga, using Saccharomyces cerevisiae (KCTC7017). From the sugar solutions prepared by the four different hydrolysis methods, bioethanol production ranged from 11.0 g/100 g dried material (acid hydrolysis) to 22.3 g/100 g dried material (enzymatic hydrolysis, EH). Bioethanol was fermented from a highly concentrated sugar solution obtained by decompression-mediated (vacuum) enrichment method (VE)...
April 3, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28372035/immunosuppressive-effects-of-bryoria-sp-lichen-forming-fungus-extracts-via-inhibition-of-cd8-t-cells-proliferation-and-il-2-production-in-cd4%C3%A2-%C2%BA-t-cells
#8
Yun-Ho Hwang, Sung-Ju Lee, Kyung-Yun Kang, Jae-Seoun Hur, Sung-Tae Yee
Lichen forming fungi are known to have various biological activities, such as antioxidant, antimicrobial, antitumor, antiviral, anti-inflammation, anti-proliferative activities. However, their immunosuppressive effects of Bryoria sp. extract (BSE) have not previously been investigated. In this study, the inhibitory activity of BSE extract on the proliferation of CD8⁺ T cells and the mixed lymphocytes reaction (MLR) was evaluated in vitro. BSE is non-toxic in spleen cells and suppresses the growth of splenocytes induced by anti-CD3...
April 3, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28372034/molecular-identification-enzyme-assay-and-metabolic-profiling-of-trichoderma-spp
#9
Soo-Jung Bae, Young-Hwan Park, Hyeun-Jong Bae, Junhyun Jeon, Hanhong Bae
The goal of this study was to identify and characterize the selected Trichoderma isolates by metabolic profiling and enzyme assay for evaluation of their potential as biocontrol agents against plant pathogens. Trichoderma isolates were obtained from the Rural Development Administration (RDA) Genebank Information Center (GIC) (Wanju, Republic of Korea). Eleven Trichoderma isolates were re-identified using ribosomal DNA internal transcribed spacer (ITS) regions. ITS sequence results showed new identification of Trichoderma isolates...
April 3, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28335590/roles-of-budding-yeast-hrr25-in-recombination-and-sporulation
#10
Min-Su Lee, Jeong Hwan Joo, Keunpil Kim
Hrr25, a casein kinase 1 δ/ε homolog in budding yeast, is essential to set up mono-orientation of sister kinetochores during meiosis. Hrr25 kinase activity coordinates sister chromatid cohesion via cohesin phosphorylation. Here, we investigated the prophase roles of Hrr25 using the auxin-inducible degron system and by ectopic expression of Hrr25 during yeast meiosis. Hrr25 mediates nuclear division in meiosis I but does not affect DNA replication. We also found that initiation of meiotic double-strand breaks as well as joint molecule formation were normal in HRR25-deficient cells...
March 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28335589/global-functional-analysis-of-butanol-sensitive-escherichia-coli-and-its-evolved-butanol-tolerant-strain
#11
Haeyoung Jeong, Seung-Won Lee, Sun Hong Kim, Eun-Youn Kim, Sinyeon Kim, Sung Ho Yoon
Butanol is a promising alternative to ethanol and is desirable for use in transportation fuels and additives to gasoline and diesel fuels. Microbial production of butanol is challenging primarily due to its toxicity and low titer of production. Herein, we compared the transcriptome and phenome of wild-type Escherichia coli and its butanol-tolerant evolved strain to understand the global cellular physiology and metabolism responsible for butanol tolerance. When the ancestral butanol-sensitive E. coli was exposed to butanol, gene activities involved in respiratory mechanisms and oxidative stress were highly perturbed...
March 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28335588/recovery-of-nk-cd56-cd3-cells-after-1-year-of-tenofovir-therapy-for-chronic-hepatitis-b-infection
#12
Hwan Hee Lee, Hyojeung Kang, Hyosun Cho
Natural killer (NK) cells have been reported to be dysfunctional in chronic hepatitis B (CHB) infection. However, the functional recovery of NK cells was not clearly understood under anti-viral therapeutic agents in CHB. In this study, we investigated the phenotypic changes of NK(CD56+CD3-) cells in terms of their functional markers (CD16, NKG2A, NKG2D) during tenofovir therapy in CHB. The frequency of NK(CD56+CD3-) cells in CHB patients was significantly increased after 12 months of tenofovir therapy when compared to baseline...
March 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28335587/neuronatin-is-associated-with-anti-inflammatory-role-in-the-white-adipose-tissue
#13
Hye In Ka, Sora Han, Ae Lee Jeong, Sunyi Lee, Hyo Jeong Yong, Ariundavaa Boldbaatar, Hyun Jeong Joo, Su Jung Soh, Ji Young Park, Jong-Seok Lim, Myung Sok Lee, Young Yang
Neuronatin (NNAT) is known to regulate ion channels during brain development and plays a role in maintaining the structure of the nervous system. A previous in silico analysis showed that Nnat was overexpressed in the adipose tissue of an obese rodent model relative to the wild-type. Therefore, the aim of the present study was to investigate the function of Nnat in the adipose tissue. Because obesity is known to systemically induce low-grade inflammation, the Nnat expression level was examined in the adipose tissue obtained from C57BL/6 mice administered lipopolysaccharide (LPS)...
March 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28301920/burkholderia-tropica-as-a-potential-microalgal-growth-promoting-bacterium-in-the-biosorption-of-mercury-from-aqueous-solutions
#14
Ana Maráa ZÁrate, July Ziret Florez, Edgardo Angulo, Lourdes Varela-Prieto, Cherlys Infante, Fredy Barrios, Beatriz Barraza, Diego Gallardo, Jorge Valdés
The use of microalgal biomass is an interesting technology for the removal of heavy metals due to its high metal-binding capacity from aqueous solutions but the interactions with bacteria as a strategy for the removal of toxic metals have been poorly studied. The goal of the current research is to investigate the potential of Burkholderia tropica co-immobilized with Chlorella sp. in polyurethane discs for the biosorption of Hg(II) from aqueous solutions and to evaluate the influence of different Hg(II) concentrations (0...
March 17, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28301919/clostridium-difficile-toxin-a-induces-reactive-oxygen-species-production-and-p38mapk-activation-to-exert-cellular-toxicity-in-neuronal-cells
#15
Peng Zhang, Ji Hong, I Na Yoon, Jin Ku Kang, Jae Sam Hwang, Ho Kim
Clostridium difficile releases two exotoxins, toxin A and toxin B, which disrupt the epithelial cell barrier in the gut to increase mucosal permeability and trigger inflammation with severe diarrhea. Many studies have suggested that enteric nerves are also directly involved in the progression of this toxin-mediated inflammation and diarrhea. C. difficile toxin A is known to enhance neurotransmitter secretion, increase gut motility, and suppress sympathetic neurotransmission in the guinea pig colitis model. Although previous studies have examined the pathophysiological role of enteric nerves in gut inflammation, the direct effect of toxins on neuronal cells and the molecular mechanisms underlying toxin-induced neuronal stress remained to be unveiled...
March 17, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28301918/pseudomonas-aeruginosa-biofilm-a-programmed-bacterial-life-for-fitness
#16
REVIEW
Keehoon Lee, Sang Sun Yoon
Biofilm is a community of microbes that typically inhabits on surfaces and is encased in an extracellular matrix. Biofilms display very dissimilar characteristics to their planktonic counterparts. Biofilms are ubiquitous in the environments and influence our life tremendously in both positive and negative ways. Pseudomonas aeruginosa is a bacterium, known to produce robust biofilms. P. aeruginosa biofilms cause severe problems in immunocompromised patients including those with cystic fibrosis or wound infection...
March 17, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28297751/preparation-of-vsv-g-viral-envelope-glycoprotein-from-pichia-pastoris-enhances-transfection-of-dna-into-animal-cells
#17
Xin Liu, Ying Dong, Jingquan Wang, Long Li, Zhenmin Zhong, Yun-Pan Li, Shao-Jun Chen, Yu-Cai Fu, Wen-Can Xu, Chi-Ju Wei
Vesicular stomatitis virus G glycoprotein (VSV-G) has been widely used for pseudotyping retroviral, lentiviral and artificial viral vectors. The objective of this study was to establish a potential approach for large scale production of VSV-G. To this end, VSV-G was cloned with a N-terminal His-tag into Pichia pastoris expression vector pPIC3.5K. Three clones (Mut(s)) containing the VSV-G expression cassette were identified by PCR. All clones proliferated normally in expansion medium, while the proliferation was reduced significantly under induction conditions...
March 15, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28297750/ros-scavenging-and-anti-wrinkle-effects-of-clitocybin-a-isolated-from-the-mycelium-of-the-mushroom-clitocybe-aurantiaca
#18
Joo-Eun Lee, Ik-Soo Lee, Kwan-Chul Kim, Ick-Dong Yoo, Han-Mo Yang
Clitocybin A, an isoindolinone from Clitocybe aurantiaca, was investigated to assess its anti-wrinkle properties, through reactive oxygen species (ROS) scavenging and elastase inhibitory activities, procollagen synthesis, and matrix metalloproteinase-1 (MMP-1) expression, in human dermal fibroblast (HDF-N) cells. Clitocybin A exhibited no significant cytotoxicity up to 10 ppm in HDF-N cells, with cell viability and cell proliferation activity greater than 94.6% and 91.9%, respectively. Strong and concentration-dependent ROS radical scavenging activities of clitocybin A were observed following irradiation with UVB at 30 mJ/cm²...
March 15, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28297749/endophytic-diaporthe-sp-ed2-produces-a-novel-anti-candidal-ketone-derivative
#19
Woei Yenn Tong, Chean Ring Leong, Wen Nee Tan, Melati Khairuddean, Latiffah Zakaria, Darah Ibrahim
This study was aimed to ascertain the anti-candidal efficacy of a novel ketone derivative isolated from Diaporthe sp. ED2, an endophytic fungus residing in medicinal herb Orthosiphon stamieus Benth. The ethyl acetate extract of the fungal culture was separated by open column and reverse phase- high performance liquid chromatography. The eluent of the second peak at retention time 5.64 minute in the HPLC system was the only peak that exhibited anti-candidal activity on Kirby Bauer assay. The structure of the compound was also elucidated by nuclear magnetic resonance and spectroscopy techniques...
March 15, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28297748/bioconversion-using-lactic-acid-bacteria-ginsenosides-gaba-and-phenolic-compounds
#20
REVIEW
Na-Kyoung Lee, Hyun-Dong Paik
Lactic acid bacteria (LAB) are used as fermentation starters in vegetable and dairy products and influence the pH and flavors of foods. For many centuries, LAB have been used to manufacture fermented foods; therefore, they are generally regarded as safe. LAB produce various substances such as lactic acid, β-glucosidase, or β-galactosidase, making them useful as fermentation starters. Existing functional substances have been tried as fermentation substrates for better bioavailability or other functions. Representative materials that were bioconverted using LAB have been reported and include minor ginsenoside, γ-amino butyric acid, equol, aglycone, bioactive isoflavones, genistein, daidzein, and others...
March 15, 2017: Journal of Microbiology and Biotechnology
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