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Journal of Microbiology and Biotechnology

Jongwook Kim, Mi-Bo Kim, Jun Gon Yun, Jae Kwan Hwang
Anti-photoaging effects of standardized Siegesbeckia glabrescens extract (SGE) and its major active compound kirenol were investigated using Hs68 human dermal fibroblasts and hairless mice, respectively. UVB-irradiated hairless mice that received oral SGE (600 mg/kg/day) showed reduced wrinkle formation and skinfold thickness compared to the UVB-irradiated control. Furthermore, SGE treatment increased the mRNA levels of collagen synthesis genes (COL1A1, COL3A1, COL4A1, and COL7A1) and activated antioxidant enzyme (catalase), while suppressing the matrix metalloproteinases (MMPs; MMP-2, -3, -9, and -13) expression...
November 23, 2016: Journal of Microbiology and Biotechnology
Hee Seon Lim, In-Tae Cha, Seong Woon Roh, Hae-Hun Shin, Myung-Ji Seo
This study evaluated the effects of culture conditions, including carbon and nitrogen sources, L-monosodium glutamate (MSG), and initial pH, on gamma-aminobutyric acid (GABA) production by Lactobacillus brevis HYE1 isolated from kimchi, a Korean traditional fermented food. L. brevis HYE1 was screened by the production analysis of GABA and genetic analysis of glutamate decarboxylase gene, resulting in 14.64 mM GABA after 48 h of cultivation in MRS media containing 1% (w/v) MSG. In order to increase GABA production by L...
November 23, 2016: Journal of Microbiology and Biotechnology
Yingyiing Zhang, Bin Tang, Guocheng Du
Carbon catabolite repression is a crucial regulation in microorganism, while its characteristic in Rhizopus is unclear now. We extracted a carbon regulation gene cre that encoded a carbon catabolite repressor protein (CRE) from Rhizopus stolonifer TP-02, and studied the regulation of CRE by real-time qPCR. CRE responded to the glucose in a certain range, wherein it could significantly regulate part of cellulases genes (eg, bg and cbh2) without cbh1. Compared with the responding of cre and four cellulases genes to the carboxymethylcellulose sodium (CMC) and the simple carbon source (lactose), the effect of CRE was only related to the concentration of reducing sugars...
November 23, 2016: Journal of Microbiology and Biotechnology
Kyung Min Kim, Jaeho Lim, Jae Jung Lee, Byung-Serk Hurh, Inhyung Lee
Initially, we screened 18 A. sojae-like strains from Aspergillus spp. isolated from meju (Korean traditional fermented soybean bricks) according to their morphological characteristics. Because members of Aspergillus section Flavi are often incorrectly identified because of their phylogenetic similarity, we re-identified these strains at morphological and molecular genetic levels. Fourteen strains were finally identified as A. sojae. The isolates produced protease and α-amylase with ranges of 2.66-10.64 and 21...
November 23, 2016: Journal of Microbiology and Biotechnology
Xiang Wang, Yanqiu Huang, Yanqing Sheng, Pei Su, Yan Qiu, Caihuan Ke, Danqing Feng
Mussels are major fouling organisms causing serious technical and economic problems. In this study, antifouling activity towards mussel was found in three compounds isolated from a marine bacterium associated with the sea anemone Haliplanella sp.. This bacterial strain, called PE2 was identified as Vibrio alginolyticus using morphology, biochemical tests and phylogenetic analysis based on sequences of 16S rRNA and four housekeeping genes (rpoD, gyrB, rctB and toxR). Three small-molecule compounds (indole, 3-formylindole and cyclo (Pro-Leu)) were purified from the ethyl acetate extract of V...
November 23, 2016: Journal of Microbiology and Biotechnology
Hyun Woo Lee, Yeon Ji Kim, Sang-Jip Nam, Hoon Kim
Alternariol monomethyl ether (AME), a dibenzopyrone derivative, was isolated from Alternaria brassicae along with altertoxin II (ATX-II). The compounds were tested for the inhibitory activity of monoamine oxidase (MAO), which catalyzes neurotransmitting monoamines. AME was found to be a highly potent and selective inhibitor of human MAO-A with an IC₅₀ value of 1.71 µM; however, it was found to be ineffective for MAO-B inhibition. ATX-II was not effective for the inhibition of either MAO-A or MAO-B. The inhibition of MAO-A using AME was apparently instantaneous...
November 14, 2016: Journal of Microbiology and Biotechnology
Woo-Shik Shin, Dohoon Lee, Sangyong Kim, Yong-Seob Jeong, Gie-Taek Chun
The task of improving a fungal strain is both expensive and time-consuming due to the fact that it requires a large number of flasks and fermenters in order to obtain a library with enough diversity. In addition, fermentations (particularly those for fungal cells) are typically performed in high-volume (100~250 mL) shake-flasks. In this study, for large and rapid screening of high-yielding mutants of itaconic acid (IA)-producing Aspergillus terreus, we attempted to develop a miniaturized culture method using 12-well and 24-well microtiter plates (MTPs, working volume = 1~2 mL)...
November 14, 2016: Journal of Microbiology and Biotechnology
Hadi Bayat, Meysam Omidi, Masoumeh Rajabibazl, Suriana Sabri, Azam Rahimpour
Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPR) in association with CRISPR associated protein (Cas) is an adaptive immune system, playing a pivotal role in the defense of bacteria and archaea. Ease of handling and cost effectiveness make CRISPR-Cas system an ideal programmable nuclease tool. Recent advances in understanding the CRISPR-Cas system have tremendously improved its efficiency. For instance, it is possible to recapitulate the chronicle CRISPR-Cas from its infancy and inaugurate a developed version by generating novel variants of Cas proteins, subduing off-target effects and optimization of innovative strategies...
November 14, 2016: Journal of Microbiology and Biotechnology
Mi Yeon Kim, Changman Kim, Jungheun Moon, Jinhee Heo, Sokhee P Jung, Jung Rae Kim
Poly-lactic acid (PLA) has been highlighted as an alternative renewable polymer for the replacement of petroleum-based plastic materials, and is considered to be biodegradable. On the other hand, the biodegradation of PLA by terminal degraders, such as microorganisms, requires a lengthy period in the natural environment and its mechanism is not completely understood. PLA biodegradation studies have been conducted using mainly undefined mixed cultures, but only few bacterial strains have been isolated and examined...
November 14, 2016: Journal of Microbiology and Biotechnology
Faysal Bin Hamid, Jinsun Kim, Cha-Gyun Shin
The foamy viruses are currently considered essential for development as vectors for gene delivery. Previous studies demonstrated that prototype foamy virus (PFV) can infect and replicate prevalently in a variety of cell types for its exclusive replication strategy. But the virus-host interaction, especially PFV-transportin3 (TNPO3) is still poorly understood. To investigate the role of TNPO3 in PFV infection, we found lower virus production in TNPO3 knockdown (KD) cells comparing to wild type 293T cells. PCR analysis revealed that viral DNAs are mostly altered to circular forms: both 1-long terminal repeat (1-LTR) and 2-LTR in TNPO3 KD cells...
November 14, 2016: Journal of Microbiology and Biotechnology
Hang Liu, Linan Xie, Jing Wang, Qiannan Guo, Shengnan Yang, Pei Liang, Chengshu Wang, Min Lin, Yuquan Xu, Liwen Zhang
Beauveria bassiana infects numbers of pest species and is known to produce insecticidal substances, e.g., the nonribosomal peptides (NRPs) beauvericin and bassianolide. However, most NRPs and their biological roles in B. bassiana remain undiscovered. To identify NRPs that potentially contribute to pathogenesis, the 21 predicted NRP synthetases (NRPSs) or NRPS-like proteins of B. bassiana ARSEF2860 were primarily ranked into three functional groups: basic metabolism (7 NRPSs), pathogenicity (12 NRPSs) and unknown function (2 NRPSs)...
November 14, 2016: Journal of Microbiology and Biotechnology
Dong Seop Kang, Soo Young Moon, Hwa Jin Cho, Jong Min Lee, In-Soo Kong
Peptidyl prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds preceding prolines. We investigated the protein-protein interaction between a 22-kDa PPIase (VaFKBP22, an FK506-binding protein) and the molecular chaperone DnaK derived from Vibrio anguillarum O1 (VaDnaK) using GST pull-down assays and a bacterial two-hybrid system for in vivo and in vitro studies, respectively. Furthermore, we analyzed the three-dimensional structure of the protein-protein interaction...
November 14, 2016: Journal of Microbiology and Biotechnology
Ahmed E Gomaa, Zhiping Deng, Zhimin Yang, Liguo Shang, Yuhua Zhan, Wei Lu, Min Lin, Yongliang Yan
The complexity of the bacterial recombination system is a barrier for the construction of bacterial mutants for the further functional investigation of specific genes. Several protocols have been developed to inactivate genes from the genus Pseudomonas. Those protocols are complicated and time-consuming and mostly do not enable easy construction of multiple knock-ins/outs. The current study describes a single and double crossover-recombination system using an optimized Vector-Free Allele Exchange (VFAE) protocol for gene disruption and gene replacement in a single species of the family Pseudomonadaceae...
November 4, 2016: Journal of Microbiology and Biotechnology
Kyu Ri Lee, Sun Min Yang, Sung Min Cho, Myunghee Kim, Sung-Yong Hong, Soo Hyun Chung
Aflatoxins are classified as Group 1 (carcinogenic to humans) by the International Agency for Research on Cancer (IARC). In this study, a total of 134 fungal strains were isolated from 65 meju samples, and two fungal isolates were selected as potential aflatoxin B₁ (AFB₁)-biodetoxification fungi. These fungi were identified as Aspergillus oryzae MAO103 and A. oryzae MAO104 by sequencing the beta-tubulin gene. The two A. oryzae strains were able to degrade more than 90% of AFB1 (initial concentration: 40 µg/L) in a culture broth in 14 days...
November 4, 2016: Journal of Microbiology and Biotechnology
Xue Li, Yan-Hua Liu, Xin Zhang, Chang-Ming Ge, Ren-Zhe Piao, Wei-Dong Wang, Jun-Cui Zong, Hong-Yan Zhao
The development and utilization of crop straw biogas resources can effectively alleviate the shortage of energy, environmental pollution, and other issues. This study performed a continuous batch test at 35°C to assess methane production potential and volatile organic acid contents using the modified Gompertz equation. Illumina MiSeq platform sequencing, which is a sequencing method based on sequencing-by-synthesis, was used to compare archaeal community diversity, and denaturing gradient gel electrophoresis (DGGE) was used to analyze bacterial community diversity in rice straw, dry maize straw, silage maize straw, and tobacco straw...
November 4, 2016: Journal of Microbiology and Biotechnology
Su-Kyoung Shin, Yong-Joon Cho, Hana Yi
Transfer of Micropolyspora internatus into the genus Saccharomonospora as "Saccharomonospora internatus comb. nov." was proposed by Kurup and Greiner-Mai, but the nomenclatural change has not been validly published. While the inclusion of M. internatus in the genus Saccharomonospora was not being established, the synonymy of "Saccharomonospora internatus" and S. viridis was proposed by Greiner-Mai. A number of recent publications regarded M. internatus as a synonym of S. viridis, but the name M. internatus is still used in some cases instead of S...
November 4, 2016: Journal of Microbiology and Biotechnology
Eun-Seon Hong, Bit-Na Kim, Yang-Hoon Kim, Jiho Min
The transport of lysosomal enzymes into the lysosomes depends on the phosphorylation of their chains and the binding of the phosphorylated residues to mannose-6-phosphate receptors. The efficiency of separation depends more on the phosphodiesterases (PDEs) than on the activity of the phosphorylation of mannose residues and can be determined in vitro. PDEs play important roles in regulation of the activation of lysosomes. The expression of proteins was confirmed by Western blotting. All PDE4 series protein expression was reduced in high concentrations of rolipram...
November 4, 2016: Journal of Microbiology and Biotechnology
Ji Young Lee, Do Youn Jun, Ki Yun Kim, Eun Ji Ha, Mi Hee Woo, Jee Youn Ko, Young Ho Yun, In-Seok Oh, Young Ho Kim
Exposure of Jurkat T cell clone (J/Neo cells) to acacetin (5,7-dihydroxy-4'-methoxyflavone), which is present in barnyard millet [Echinochloa esculenta (A. Braun)] grains, resulted in cytotoxicity, accumulation of apoptotic sub-G1 cells, Bak activation, loss of mitochondrial membrane potential (Δψm), activation of caspase-9 and caspase-3, cleavage of poly (ADP-ribose) polymerase (PARP), and FITC-Annexin V-stainable phosphatidylserine exposure on the external surface of the cytoplasmic membrane without accompanying necrosis...
November 4, 2016: Journal of Microbiology and Biotechnology
Won-Yong Song, Yong-Seok Song, Hyung Won Ryu, Sei-Ryang Oh, JinTae Hong, Do-Young Yoon
In human airway, mucus exists to protect respiratory system as a primary barrier of innate immune system. However, hyper-expressed mucus limits airflow, resulting in decrease of lung function. Among more than 20 mucin family, MUC5AC and MUC5B are major glycoproteins in human airway mucus. Epidermal growth factor receptor (EGFR) signaling pathway is one of the mechanisms of these mucins expression and Specificity protein-1 (Sp1) transcription factor is the downstream signal of this pathway, playing pivotal roles in mucin expression...
October 28, 2016: Journal of Microbiology and Biotechnology
Hyo Ryoung Kim, Il Joon Lee, Dong-Eun Kim
Detection of exon 19 deletion mutation in epidermal growth factor receptor (EGFR) gene, which results in increased and sustained phosphorylation of EGFR, is important for diagnosis and treatment guideline in non-small-cell lung cancer (NSCLC). Here, we have developed a simple and convenient detection system using the interaction between G-quadruplex and fluorophore thioflavin T (ThT) for discriminating EGFR exon 19 deletion mutant DNA from wild type without label and quencher. In the presence of exon 19 deletion mutant DNA, the probe DNAs annealed to the target sequences were transformed into G-quadruplex structure...
October 28, 2016: Journal of Microbiology and Biotechnology
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