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Journal of Microbiology and Biotechnology

Da-Wool Jeong, Chi Heung Cho, Jong Suk Lee, Seung Hwan Lee, Taewan Kim, Dae-Ok Kim
Peel of astringent persimmon ( Diospyros kaki Thunb. cv. Cheongdo-Bansi) is a by-product of dried persimmon ( gotgam ).We investigated if deastringentpeel extracts of persimmon cv. Cheongdo-Bansi had antioxidative and neuroprotective properties.Two different extracts were prepared: thermallyand nonthermallytreated persimmon peel extracts (TPE and NTPE, respectively). Both TPE and NTPE were fractionated sequentially in n -hexane, chloroform, ethyl acetate, n -butanol, and water. TPE and NTPE ethyl acetate fractions had the highest total phenolic andflavonoid contents as well as antioxidant capacities among all the fractions...
June 8, 2018: Journal of Microbiology and Biotechnology
Han Sol Seo, Sunmin Lee, Digar Singh, Min Kyung Park, Young-Suk Kim, Hye Won Shin, Sun A Cho, Choong Hwan Lee
Production of good Koji primarily depends upon the selection of substrate materials and fermentative microflora, which together influence the characteristic flavor and aroma. Herein, we performed comparative metabolomic analyses of volatile organic compounds (VOCs) and primary metabolites for Koji samples fermented individually with Bacillus amyloliquefaciens and Aspergillus oryzae . The VOCs and primary metabolites were analyzed using headspace solid phase microextraction (HS-SPME) followed by gas chromatography time-of-flight mass spectrometry (GC-TOF-MS)...
June 8, 2018: Journal of Microbiology and Biotechnology
Sukjung Choi, Gwi-Deuk Jin, Jongbin Park, Inhwan You, Eun Bae Kim
Lactobacillus plantarum is a lactic acid bacterium that promotes animal intestinal health as a probiotic and is found in a wide variety of habitats. Here, we investigated the genomic features of different clusters of L. plantarum strains via pan-genomic analysis. We compared the genomes of 108 L. plantarum strains that were available from the NCBI GenBank database. These genomes were 2.9-3.7 Mbp in size and 44-45% in G+C content. A total of 8,847 orthologs were collected, and 1,709 genes were identified to be shared as core genes by all the strains analyzed...
June 8, 2018: Journal of Microbiology and Biotechnology
Hyesook Hyun, Sunjin Lee, Jong Suk Lee, Kyungyun Cho
DKxanthenes area class of yellow secondary metabolites produced by myxobacterial genera Myxococcus and Stigmatella . We identified a putative 49.5 kb DKxanthene biosynthetic gene cluster from M. stipitatus DSM 14675 by genomic sequence and mutational analysis. The cluster was comprisedof 15 genes (MYSTI_06004-MYSTI_06018) encoding polyketide synthases, non-ribosomal peptide synthases, and proteins with unknown functions. Disruption of the genes by plasmid insertion resulted in defects in the production of yellow pigments...
June 8, 2018: Journal of Microbiology and Biotechnology
Zahra Yahaghi, Mehran Shirvani, Farshid Nourbakhsh, Teodoro Coba de la Peña, José J Pueyo, Majid Talebi
The aim of this study was to isolate and characterize lead (Pb)-solubilizing bacteria from heavy metal-contaminated mine soils and to evaluate their inoculation effects on growth and Pb absorption of Brassica juncea . The isolates were also evaluated for their plant growth promoting characteristics, as well as heavy metal and salt tolerance. A total of 171 Pb-tolerant isolates were identified, of which only 15 bacterial strains were able to produce clear haloes in solid medium containing PbO or PbCO₃, indicating Pb solubilization...
June 8, 2018: Journal of Microbiology and Biotechnology
Xiaojian Ma, Chongde Wu, Jun Huang, Rongqing Zhou, Bi Shi
The aim of this study was to investigate the microbial community of three tannery wastewater treatment plants (WWTPs) involved in nitrification by IlluminaMiSeq sequencing. The results showed that highly diverse communities were observed intannery wastewater. A total of 6 phyla including Proteobacteria (37-41%), Bacteroidetes (6.04-16.80), Planctomycetes (3.65-16.55), Chloroflexi (2.51-11.48), Actinobacteria (1.91-9.21), and Acidobacteria (3.04-6.20) were identified as the main phyla, and Proteobacteria dominated in all thesamples...
June 8, 2018: Journal of Microbiology and Biotechnology
Feng Liu, Yue-Ping Cao
Glyphosate inhibits the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) in the shikimate pathway. A mutant of EPSPS from Pantoea sp. was identified using site-directed mutagenesis (SDM). The mutant significantly improved glyphosate resistance. The mutant had mutations in three amino acids: Gly97 to Ala, Thr 98 to Ile and Pro 102 to Ser. These mutation sites in E.coli have been studied as significant active sites of glyphosate resistance. However, in our research they were found to jointly contribute to the improvement of glyphosate tolerance...
June 6, 2018: Journal of Microbiology and Biotechnology
Lifang Feng, Kuo Zhang, Mengsha Gao, Chunwei Shi, Caiyun Ge, Daofeng Qu, Junli Zhu, Yugang Shi, Jianzhong Han
Vibrio parahaemolyticus contamination, causes serious foodborne illness, and it has become a global health problem. As a disinfectant, aqueous ozone can effectively kill a number of bacteria, viruses, parasites and other microorganisms. In this study, three factors, namely, the aqueous ozone concentration, the exposure time, and the bacterial density, were analyzed by response surface methodology, and the aqueous ozone concentration was the most influential factor in the sterilization ratio. Under low aqueous ozone concentrations (less than 0...
June 6, 2018: Journal of Microbiology and Biotechnology
Siyeon Kim, Kyo Eun Kook, Changhee Kim, Jae-Kwan Hwang
Periodontal disease is triggered by the host immune response to pathogens in the microbial biofilm. Worsening of periodontal disease destroys the tooth-supporting tissues and alveolar bone. As oral inflammation can induce systemic diseases in humans, it is important to prevent periodontal disease. In this study, we demonstrated that Curcuma xanthorrhiza supercritical extract(CXS) and its active compound, xanthorrhizol (XAN), exhibit anti-inflammatory effects on lipopolysaccharide (LPS)-treated HGF-1 cells and anti-osteoclastic effects on receptor activator of nuclear factor kappa B ligand (RANKL)-treated RAW264...
June 6, 2018: Journal of Microbiology and Biotechnology
Song-Yi Kim, MIn Kyung Song, Ju Hyun Jeon, Joong-Hoon Ahn
Phenylethanoids, including 2-phenylethanol, tyrosol, and salidroside are a group of phenolic compounds with a C6-C2 carbon skeleton synthesized by plants. Phenylethanoids display a variety of biological activities, including antibacterial, anticancer, anti-inflammatory, neuroprotective, and anti-asthmatic activities. Recently, successful microbial synthesis of phenylethanoids through metabolic engineering and synthetic biology approaches has been reported and could allow phenylethanoid production from alternative microbial sources...
June 6, 2018: Journal of Microbiology and Biotechnology
Wen Zhao, Jian Zhang, Yunyun Jiang, Xiao Zhao, Xiaona Hao, Liu Li, Zhennai Yang
The exopolysaccharide (EPS) produced by Bacillus amyloliquefaciens GSBa-1 was isolated and purified by ethanol precipitation, DEAE-cellulose and Sepharose CL-6B chromatography. The molecular weight of the purified EPS was determined to be 54 kDa. Monosaccharide analysis showed that the EPS was composed of predominantly glucose, and it was further confirmed by NMR spectroscopy to be α-glucan consisted of a trisaccharide repeating unit with possible presence of two α-(1-->3) and one α-(1-->6) glucosidic linkages...
June 6, 2018: Journal of Microbiology and Biotechnology
Ling Li, Seul Ah Kim, Ruosi Fang, Nam Soo Han
Phosphomannomutase (ManB) converts mannose-6-phosphate (M-6-P) to mannose-1-phosphate (M-1-P), which is a key metabolic precursor for the production of GDP-D -mannose used for production of glycoconjugates and post-translational modification of proteins. The aim of this study was to express the manB gene from Escherichia coli in Lactococcus lactis subsp. cremoris NZ9000 and to characterize the encoded enzyme.The manB gene from E. coli K12, of 1371 bp and encoding 457 amino acids (52 kDa), was cloned and overexpressed in L...
June 6, 2018: Journal of Microbiology and Biotechnology
Yoonju Lee, Youngjae Cho, Eiseul Kim, Hyun-Joong Kim, Hae-Yeong Kim
Jeotgal is Korean traditional fermented seafood with a high concentration of salt. In this study, we isolated lactic acid bacteria (LAB) from galchi ( Trichiurus lepturus , hairtail) and myeolchi ( Engraulis japonicas , anchovy) jeotgal on MRS agar and MRS agar containing 5% NaCl (MRS agar+5% NaCl), followed by identification using 16S rRNA sequencing and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI TOF-MS) asculture-dependent methods. We also performed polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to identify bacterial communities as a culture-independent method...
June 6, 2018: Journal of Microbiology and Biotechnology
Franciane Cabral Pinheiro, Vandreza Cardoso Bortolotto, Stífani Machado Araujo, Márcia Rósula Poetini, Carla Pohl Sehn, José S S Neto, Gilson Zeni, Marina Prigol
This study aimed to evaluate the antimicrobial activity of 2-phenylethynyl-butyltellurium (PEBT) in Escherichia coli and the relation to its pro-oxidant effect. For this, we carried out disk diffusion test, minimum inhibitory concentration (MIC) assay, and survival curve analysis. We also measured the level of extracellular reactive oxygen species (ROS), activity of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), and level of non-protein thiols (NPSH). PEBT at 1.28 and 0.128 mg/disk exhibited antimicrobial capability in disk diffusion test, with MIC value of 1...
May 25, 2018: Journal of Microbiology and Biotechnology
Xiaowen Lv, Huanru Wang, Airong Su, Ying Chu
Silver nanoparticles have widely applied for biomedical areas due to its potent antiviral and anti-bacterial activity. Synthesis of silver nanoparticles using biomacromoleculesis more efficient, environment-friendly and cost-saving compare with traditional approach. In this paper, a novel approach was developed to establish a reaction system with Ag+ -BH4- -sericin to synthesize silver nanoparticles conjugated to sericin (AgNPs-Sericin). Sericin could be as a good dispersant and stabilizing agent, which is able to modify nanoscale-silver particles, the average diameter of which was only 3...
May 25, 2018: Journal of Microbiology and Biotechnology
Pil-Won Seo, Ho-Chang Ryu, Do-Heon Gu, Hee-Sae Park, Suk-Youl Park, Jeong-Sun Kim
2-Keto-3-deoxy-6-phosphogluconate (KDPG) aldolase, which catalyzes aldol cleavage and condensation reactions, has two distinct substrate-binding sites.The substrate-binding mode at the catalytic site and Schiff-base formation have been well studied. However, structural information on the phosphate-binding loop (P-loop) is limited. Zymomonas mobilis KDPG aldolase is one of the aldolases with a wide substrate spectrum. Its structure in complex with the substrate-mimicking 3-phosphoglycerate (3PG) shows that the phosphate moiety of 3PG interacts with the P-loop and a nearby conserved serine residue...
May 25, 2018: Journal of Microbiology and Biotechnology
Ho Sik Rho, Kyungoh Choi
Oxidoreductases are effective biocatalysts, but their practical use is limited by the need for large quantities of NAD(P)H. In this study, a whole-cell biocatalyst for NAD(P)H cofactor regeneration was developed using the economical substrate glycerol. This cofactor regeneration system employs permeabilized Escherichia coli cells in which the glpD and gldA genes were deleted and the gpsA gene, which encodes NAD(P)+ -dependent glycerol-3-phosphate dehydrogenase (G3PDH), was overexpressed. These manipulations were applied to block a side reaction (i...
May 25, 2018: Journal of Microbiology and Biotechnology
Gyunghee Jo, Mun Sik Jeong, Jimin Wi, Doo Hyun Kim, Sangkyu Kim, Dain Kim, Jun-Yeol Yoon, Heesu Chae, Kyun-Hwan Kim, Hyo Jeong Hong
The hepatitis B virus (HBV) envelope contains small (S), middle (M), and large (L) proteins. PreS1 of the L protein contains a receptor-binding motif crucial for HBV infection. This motif is highly conserved among 10 HBV genotypes (A-J), making it a potential target for the prevention of HBV infection. In this study, we successfully generated a neutralizing human monoclonal antibody (mAb), 1A8 (IgG1), that recognizes the receptor-binding motif of preS1 using a phage-displayed human synthetic Fab library. Analysis of the antigen-binding activity of 1A8 for different genotypes indicates that it can specifically bind to the preS1 of major HBV genotypes (A-D)...
May 25, 2018: Journal of Microbiology and Biotechnology
Jose F Garcia-Mazcorro, Romina Pedreschi, Boon Chew, Scot E Dowd, Jorge R Kawas, Giuliana Noratto
Raspberries are polyphenol-rich fruits with the potential to reduce the severity of the clinical signs associated with obesity, a phenomenon that may be related to changes in the gut microbiota. The aim of this study was to investigate the effect of raspberry supplementation on the fecal microbiota using an in vivo model of obesity. Obese diabetic db/db mice were used in this study and assigned to two experimental groups (with and without raspberry supplementation). Fecal samples were collected at the end of the supplementation period (8 weeks) and used for bacterial 16S rRNA gene profiling using aMiSeq instrument (Illumina)...
May 25, 2018: Journal of Microbiology and Biotechnology
Yago Queiroz Dos Santos, Bruno Oliveira de Veras, Anderson Felipe Jácome de França, Krystyna Gorlach-Lira, Jannaina Velasques, Ludovico Migliolo, Elizeu Antunes Dos Santos
A salt-tolerant cellulase secreted by a marine Bacillus sp. SR22 strain with wide resistance to temperature and pH was purified and characterized. The approximate mass was 37 kDa endoglucanase named as Bc22Cel was purified by ammonium sulphate precipitation, gel filtration chromatography and extraction from the gel after non-reducing sodium dodecylsufate-polyacrylamide gel electrophoresis. The optimal pH value and temperature of Bc22Cel were 6.5 and 60 °C, respectively. The purified Bc22Cel showed a considerable halophilic property being able to maintain more than 70% of residual activity even when pre-incubated with 1...
May 11, 2018: Journal of Microbiology and Biotechnology
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