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Journal of Microbiology and Biotechnology

Han Young Chung, Kyu-Ho Lee, Sangryeol Ryu, Hyunjin Yoon, Ju-Hoon Lee, Hyeun Bum Kim, Heebal Kim, Hee Gon Jeong, Sang Ho Choi, Bong-Soo Kim
Bacillus cereus causes food-borne illness through contaminated foods; therefore, its pathogenicity and genome sequences have been analyzed in several studies. We sequenced and analyzed the B. cereus strain FORC_021 isolated from a sashimi restaurant. The genome sequence consists of 5,373,294 bp with 35.36% GC contents, 5,350 predicted CDSs, 42 rRNA genes, and 107 tRNA genes. Based on in silico DNA-DNA hybridization values, B. cereus ATCC 14579(T) was closest to FORC_021 among the complete genome-sequenced strains...
October 12, 2016: Journal of Microbiology and Biotechnology
Mi-Kyung Park, Bryan A Chin
To date there has been no employment of a magnetoelastic (ME) biosensor method to detect S. Typhimurium in soil. The ME biosensor method needs to be investigated and modified for its successful performance. Filtration, cation-exchange resin method, and combinations of both methods were employed for the extraction of S. Typhimurium from soil, and the number of S. Typhimurium and the resonant frequency shift of the ME sensor were then compared using a brilliant-green-sulfa agar plate and an HP 8751A network analyzer...
October 12, 2016: Journal of Microbiology and Biotechnology
Anawat Pakdeesuwan, Tomohiro Araki, Sakda Daduang, Wisarut Payoungkiattikun, Nisachon Jangpromma, Sompong Klaynongsruang
The hydrolysis of proteins constitutes an invaluable tool granting access to a variety of peptide fragments with potentially interesting biological properties. Therefore, hemoglobin (Hb) hydrolysate of Crocodylus siamensis was generated by digestion under acidic conditions. The antibacterial and antioxidant activity of Hb hydrolysate were assessed in comparison with intact Hb. A disc diffusion assay revealed that the Hb hydrolysate exhibited antibacterial activity against eight strains of Gram-positive bacteria and showed a higher efficacy than intact Hb...
October 6, 2016: Journal of Microbiology and Biotechnology
Ki Seong Eom, Jin Sung Cheong, Seung Jae Lee
Zinc finger proteins are among the most extensively applied metalloproteins in the field of biotechnology due to their unique structural and functional aspects as transcriptional and translational regulators. The classical zinc fingers are the largest family of zinc proteins and they provide critical roles in physiological systems from prokaryotes to eukaryotes. Two cysteine and two histidine residues (Cys₂His₂) coordinate to the zinc ion for the structural functions to generate a ββα fold, and this secondary structure supports specific interactions with their binding partners including DNA, RNA, lipids, proteins, and small molecules...
October 6, 2016: Journal of Microbiology and Biotechnology
Rita Terezinha de Oliveira Carneiro, Maíza Alves Lopes, Marília Lordelo Cardoso Silva, Verônica da Silva Santos, Volnei Brito de Souza, Aurizangela Oliveira de Sousa, Carlos Priminho Pirovani, Maria Gabriela Bello Koblitz, Raquel Guimarães Benevides, Aristóteles Góes-Neto
White-rot basidiomycetes are the organisms that decompose lignin most efficiently and Trametes villosa is a promising species for the ligninolytic enzymes production. There are several publications on Trametes villosa applications for lignin degradation regarding the expression and secretion of laccase (Lac) and manganese peroxidase (MnP) but no reports on the identification and characterization of lignin peroxidase (LiP), a relevant enzyme for the efficient breaking down of lignin. The object of this study was to identify and partially characterize, for the first time, gDNA, mRNA and the corresponding lignin peroxidase (TvLiP) protein from a strain of Trametes villosa CCMB561 from the Brazilian semi-arid region...
October 6, 2016: Journal of Microbiology and Biotechnology
Tae-Hyoung Kim, Oh Joo Kweon, Hye Ryoun Kim, Mi-Kyung Lee
Recently, several studies have revealed that commercial microbial identification systems do not accurately identify the uncommon causative species of candidiasis, including Candida famata, Meyerozyma guilliermondii, and C. auris. We investigated the accuracy of species-level identification in a collection of clinical isolates previously identified as C. famata (N=38), C. lusitaniae (N=12), and M. guilliermondii (N=5) by the Vitek 2 system. All 55 isolates were re-analyzed by the Phoenix system (Becton Dickinson Diagnostics), two matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analyzers (a Vitek MS and a Bruker Biotyper), and by sequencing of internal transcribed space (ITS) regions or 26S rRNA gene D1/D2 domains...
October 6, 2016: Journal of Microbiology and Biotechnology
Kai Zhao, Jiwei Duan, Xueling Ma, Yue Zhang, Xiaohua Wang
Termites are destructive to agriculture, forestry and buildings, but they can also promote agro-ecosystem balance through the degradation of lignocellulose. Termite-triggered cellulose digestion may be clarified through microbial metabolism of cellulose products. In the present study, we characterized the activities of cellulase and its three components synthesized by the cellulase-producing fungal strain HDZK-BYTF620 isolated from the gut of Odontotermes formosanus. The protein components of cellulase were synthesized by strain HDZK-BYTF620, which were isolated and characterized using polyacrylamide gel electrophoresis, and the expression of cellulose was studied at the proteome level...
October 6, 2016: Journal of Microbiology and Biotechnology
Hui Li, Jun Huang, Xinping Liu, Rongqing Zhou, Xiaofei Ding, Qianyin Xiang, Liqiang Zhang, Chongde Wu
It is vital to understand the changing characteristics of interphase microbial communities and interspecies synergisms during the fermentation of Chinese liquors. In this study, microbial communities in the three indispensable phases (pit mud, zaopei and huangshui) of Luzhou-flavored liquor manufacturing pits and their shifts during cellars use were first investigated by polyphasic culture-independent approaches. The archaeal and eubacterial communities in the three phases were quantitatively assessed by combined phospholipid ether lipids (PLEL)/ phospholipid fatty acid (PLFA) analysis and fluorescence in situ hybridization (FISH)...
October 6, 2016: Journal of Microbiology and Biotechnology
Zewang Guo, Xihua Zhao, Yuanzhi He, Tianxing Yang, Huifang Gao, Ganxi Li, Feixue Chen, Meijing Sun, Jung-Kul Lee, Liaoyuan Zhang
Acetoin (AC) is a volatile platform compound with various potential industrial applications. AC contains two stereoisomeric forms: (3S)-AC and (3R)-AC. Optically pure AC is important potential intermediate and widely used as a precursor to synthesize novel optically materials. In this study, chiral (3R)-AC production from meso-2,3-butanediol (meso-2,3-BD) was obtained using recombinant Escherichia coli cells co-expressing meso-2,3-butanediol dehydrogenase (meso-2,3-BDH), NADH oxidase (NOX) and hemoglobin protein (VHB) from Serratia sp...
October 6, 2016: Journal of Microbiology and Biotechnology
Eun-Jin Kim, Hyun-Joo Chang, Soojin Kwak, Jong-Hyun Park
Characteristics of E.coli O157:H7 specific infection bacteriophages (O157 coliphages) and broad-host-range bacteriophages for other E.coli serotypes (broad-host coliphages) were compared. The burst sizes of two groups were ranged from 40 to 176 PFU/infected. Distribution of virulence factors of stx1, stx2, ehxA, and saa between two groups were not differenciated. Broad host coliphages showed a little stable at 70°C than O157 coliphages. However, O157 coliphages showed high acid and ethanol tolerant by reduction of only 22% and 11% phages under pH 3 and 70% ethanol for an hour exposure...
October 6, 2016: Journal of Microbiology and Biotechnology
Hyeon-Jeong Son, Kyung Bin Song
This study examined the antimicrobial activity of flaxseed meal extract (FME) against Staphylococcus aureus and Escherichia coli O157:H7 inoculated on red mustard. With the treatment of 0.7% FME for 3 min, the reduction levels of S. aureus and E. coli O157:H7 populations were 1.23 and 1.83 log CFU/g, respectively. In addition, the combined treatment of 0.7% FME at 50°C for 3 min reduced the populations of the pathogenic bacteria by 2.28 and 2.41 log CFU/g, respectively. Color and vitamin C content were not significantly different between treatments...
October 6, 2016: Journal of Microbiology and Biotechnology
Viviana P Rubio, Alejandra Bravo, Jorge Olmos
In this work we isolated a Surface-Layer-Protein (SLP) from a Bacillus thuringiensis (Bt) strain to evaluate it cytotoxic effects against MDA-MB-231 human breast cancer cells. AP11 was selected from a group of Bt strains using SLP oligonucleotides developed from Bacillus conserved regions. AP11 strain was grown in Luria Bertani (LB) medium until late exponential phase; an 86 kDa protein was extracted using 5 M LiCl and identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). It corresponded to a multispecies S-layer protein highly similar to previously described SLP in B...
October 6, 2016: Journal of Microbiology and Biotechnology
Yajun Chen, Lin Ye, Fuzheng Zhao, Lin Xiao, Shupei Cheng, Xu-Xiang Zhang
Oxidation ditch (OD) is one of the most widely used processes for treating municipal wastewater. However, the microbial communities in the OD systems have not been well characterized and little information about the shift of bacterial community during the startup process of the OD systems is available. In this study, we investigated the bacterial community changes during the startup period (over 100 days) of a full-scale OD. The results showed that the bacterial community dramatically changed during the startup period...
October 6, 2016: Journal of Microbiology and Biotechnology
Wen Ren, Qun Zhang, Xuenan Liu, Shuguo Zheng, Lili Ma, Feng Chen, Tao Xu, Baohua Xu
As one of the most complex human-associated microbial habitats, the oral cavity harbors hundreds of bacteria. Halitosis is a prevalent oral condition that is typically caused by bacteria. The aim of this study was to analyze the microbial communities and predict functional profiles in supragingival plaque from healthy individuals and those with halitosis. Ten preschool children were enrolled in this study; five with halitosis and five without. Supragingival plaque was isolated from each participant and 16S rRNA gene pyrosequencing was used to identify the microbes present...
September 23, 2016: Journal of Microbiology and Biotechnology
Hongyan Yang, Hao Wu, Lijuan Gao, Hongbai Jia, Yuan Zhang, Zongjun Cui, Yuhua Li
To investigate the effects of Lactobacillus curvatus and Leuconostoc mesenteroides on suan cai fermentation, Lactobacillus curvatus and/or Leuconostoc mesenteroides were inoculated into suan cai. Physicochemical indexes were measured, and the microbial dynamics during the fermentation were analyzed by Illumina MiSeq sequencing and quantitative polymerase chain reaction (qPCR). The results showed that inoculation with lactic acid bacteria (LAB) lowered the pH of the fermentation system more rapidly. The decrease in water-soluble carbohydrates in the inoculated treatments occurred more rapidly than in the control...
September 23, 2016: Journal of Microbiology and Biotechnology
Hyunwoo Jeon, Pradeepraj Durairaj, Dowoo Lee, Md Murshidul Ahsan, Hyungdon Yun
Fungal cytochrome P450 (CYP) enzymes catalyze versatile monooxygenase reactions and plays major share in fungal adaptations due to their essential roles in the production of metabolites critical for pathogenesis, detoxification of xenobiotics, and exploitation of substrates. Though, fungal CYP dependent biotransformation for the selective oxidation of organic compounds in yeast system is advantageous, it often suffers due to the shortage of intracellular NADPH. In this study, we aimed to investigate the use of bacterial glucose dehydrogenase (GDH) for the intracellular electron regeneration of fungal CYP monooxygenase in a yeast reconstituted system...
September 23, 2016: Journal of Microbiology and Biotechnology
Wahyu Sri Kunto Nugroho, Dong-Woo Kim, Jong-Cheol Han, Young Baek Hur, Soo-Wan Nam, Hak Jun Kim
Most cold-adapted enzymes possess higher Km and kcat values than those of their mesophilic counterparts to maximize the reaction rate. This characteristic is often ascribed to a high structural flexibility and improved dynamics in the active site. However, this may be less convincing to cold-adapted metabolic enzymes, which work at substrate concentrations near Km. In this respect, cold adaptation of a shikimate kinase (SK) in the shikimate pathway from psychrophilic Colwellia psychrerythraea (CpSK) was characterized by comparing it with a mesophilic Escherichia coli homolog (EcSK)...
September 23, 2016: Journal of Microbiology and Biotechnology
Liaoyuan Zhang, Zewang Guo, Hufang Gao, XiaoQian Peng, Yongyu Li, Shujing Sun, Jung-Kul Lee, Wenxiong Lin
Many plant-pathogenic bacteria were dependent on quorum sensing (QS) to evoke disease. In this study, the population of QS and quorum quenching (QQ) bacteria was analyzed in consecutive monoculture system of Pseudostellaria heterophylla. The isolated QS strains were identified as Serratia marcescens with SwrIR-type QS system and exhibited a significant increase over the years of monoculture. Only one QQ strain was isolated from new planted soil sample and identified as Bacillus thuringiensis which secreted lactonase to degrade QS signal molecules...
September 23, 2016: Journal of Microbiology and Biotechnology
Zhongyang Sun, Jia Deng, Haizhen Wu, Qiyao Wang, Yuanxing Zhang
Edwardsiella tarda is a Gram-negative pathogenic bacterium in aquaculture which can cause hemorrhagic septicemia in fish. Many secreted proteins have been identified as virulent factors of E. tarda. While the virulent phenotype is based on the expression regulation of the virulent genes. It is important to understand the expression profile of the virulent genes. Quantitative RT-PCR is one of the preferred methods for determining different gene expressions. It is important to select the most stable gene as a reference in E...
September 23, 2016: Journal of Microbiology and Biotechnology
Taeyoung Kim, Sukwon Kang, Je Hoon Sung, Young Koo Kang, Young Hwa Kim, Jae Kyung Jang
Polyester cloth (PC) was selected as a prospective inexpensive substitute separator material for microbial fuel cells (MFCs). PC was compared with a traditional Nafion proton exchange membrane (PEM) as an MFC separator by analyzing its physical and electrochemical properties. A single layer of PC showed higher mass transfer (e.g., for O₂/H⁺/ions) than the Nafion PEM; in the case of oxygen mass transfer coefficient (ko), a rate of 50.0 × 10⁻⁵ cm·s⁻¹ was observed compared to a rate of 20.8 × 10⁻⁵ cm·s⁻¹ in the Nafion PEM...
September 23, 2016: Journal of Microbiology and Biotechnology
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