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Journal of Microbiology and Biotechnology

Shabir Ahmad, Seung Yeup Lee, Raees Khan, Hyun Gi Kong, Geun Ju Son, Nazish Roy, Kihyuck Choi, Seon-Woo Lee
Ralstonia solanacearum causes bacterial wilt in a wide variety of host plant species and produces a melanin-like blackish-brown pigment in stationary phase when grown in minimal medium supplemented with tyrosine. To study melanin production regulation in R. solanacearum, five mutants exhibiting over-production of melanin-like pigments were selected from a transposon (Tn) insertion mutant library of R. solanacearum SL341. Most of the mutants except one (SL341T) were not complemented by the original gene or over-produced melanins...
July 27, 2017: Journal of Microbiology and Biotechnology
Juyoun Shin, Jin Yang Baek, Doo Ryeon Chung, Kwan Soo Ko
In this study, we characterized the blaNDM-5-bearing plasmid in a Klebsiella pneumoniae isolate that had lost the plasmid during serial passage. We determined the complete sequences of the plasmid pCC1410-2, which was extracted from a K. pneumoniae ST709 isolate collected at a Korean hospital from which two NDM-5-producing K. pneumoniae isolates were subsequently isolated. As a result, the pCC1410-2 plasmid had a backbone structure that was similar to those of two plasmids previously reported from the same hospital, but lacked some antibiotic resistance genes (blaTEM-1, rmtB, mphR(A), mrx(A), and mph(A))...
July 27, 2017: Journal of Microbiology and Biotechnology
Seon Yeong Byeon, Myung Kwon Cho, Kyou Hee Shim, Hye Jin Kim, Hyeon Gi Song, Hwa Sung Shin
Cosmetic patches have recently been developed as skin products for personal care due to rapid advances in the technology of delivery of active ingredients, moisture and adhesiveness to skin. Alginate and Spirulina are typical marine resources used in cosmetic products. This research involved the development of a Spirulina extract-impregnated alginate nanofiber cosmetic patch supported by a polycaprolactone (PCL) nanofiber cover (Spi/Alg-PCL NF patch). In addition to the ability of alginate to affect moisture and adhesiveness to skin, the impregnation of Spirulina extract strengthened those abilities as well as its own bioactive effectiveness...
July 14, 2017: Journal of Microbiology and Biotechnology
Sivachandiran Somasundaram, Murali Kannan Maruthamuthu, Irisappan Ganesh, Gyeong Tae Eom, Soon Ho Hong
Gamma-aminobutyric acid is a precusor of nylon-4, which is a promising heat-resistant biopolymer. GABA can be produced from the decarboxylation of glutamate by glutamate decarboxylase. In this study, a synthetic scaffold complex strategy was employed involving Neurospora crassa glutamate decarboxylase (GadB) and Escherichia coli GABA antiporter (GadC) to improve GABA production. To construct the complex, the SH3 domain was attached to the N. crassa GadB, and SH3 ligand was attached to the N-terminus, middle and C-terminus of E...
July 14, 2017: Journal of Microbiology and Biotechnology
Chao Tong, Ning Chen, Xun Liao, Xuemei Yuan, Mengjiao Sun, Xiaoliang Li, Weihuan Fang
Classical swine fever virus (CSFV) is the etiologic agent of classical swine fever (CSF), a highly contagious disease that causes significant economic losses to the swine industry. The lapinized C-strain, a widely used vaccine strain against CSFV, has low growth efficiency in cell culture, thus limits the productivity in vaccine industry. In this study, a recombinant virus derived from C-strain was constructed and subjected to continuous passaging in PK-15 cells with the goal of acquiring high progeny virus yield...
July 14, 2017: Journal of Microbiology and Biotechnology
Guiqiang He, Chongde Wu, Jun Hunag, Rongqing Zhou
This study investigated the effect of proline addition on the salt tolerance of Tetragenococcus halophilus. Salt stress led to the accumulation of intracellular proline in T. halophilus. When 0.5 g/L proline was added to hyperhaline medium, the biomass increased 34.6% (12% NaCl) and 27.7% (18% NaCl) compared with the control (without proline addition), respectively. Metabolomic approach was employed to reveal the cellular metabolic responses and protective mechanisms of proline upon salt stress. The results showed that both cellular membrane fatty acid composition and metabolite profiling were responded by increasing unsaturated and cyclopropane fatty acid proportions, as well as accumulating some specific intracellular metabolites (environmental stress protector)...
July 7, 2017: Journal of Microbiology and Biotechnology
Seulgi Choi, Hyelim Park, Marstella Minelko, Eun-Kyung Kim, Mi-Ra Cho, Jae-Hwan Nam
IK can downregulate interferon (IFN)-γ-induced major histocompatibility complex (MHC) class II expression through the MHC class II transactivator, which suggests that IK can inhibit the interactions between immune cells. We delivered adeno-associated virus serotype 2 (AAV2) encoding the genes for truncated IK (tIK) or green fluorescent protein (GFP) to DBA1/J mice via intravenous injection. Seven weeks after injection, collagen-induced arthritis (CIA) was induced in the AAV2 treated mice. AAV2-tIK injection reduced the severity of arthritis and the percentage of pathogenic Th17 cells compared with AAV2-GFP injection...
July 7, 2017: Journal of Microbiology and Biotechnology
Won-Heong Lee, Yong-Su Jin
In simultaneous saccharification and fermentation (SSF) for production of cellulosic biofuels, engineered Saccharomyces cerevisiae capable of fermenting cellobiose has provided several benefits such as lower enzyme costs and faster fermentation rate compared with wild-type S. cerevisiae fermenting glucose. In this study, the effects of an alternative intracellular cellobiose utilization pathway-a phosphorolytic pathway based on a mutant cellodextrin transporter [CDT-1 (F213L)] and cellobiose phosphorylase (SdCBP)-was investigated by comparing with a hydrolytic pathway based on the same transporter and an intracellular β-glucosidase (GH1-1) for their SSF performances under various conditions...
July 7, 2017: Journal of Microbiology and Biotechnology
Sangnam Oh, Mi Ri Park, Sangdon Ryu, Brighton E Maburutse, Ji-Uk Kim, Younghoon Kim
MicroRNAs (miRNAs) are abundant in bovine milk and milk derived from other livestock, and they have functional roles in infants and in the secretion process of mammary glands. However, few studies have evaluated miRNAs in dairy processes, such as during cheese making and ripening. Thus, we investigated the characteristics of milk-derived miRNAs during the manufacturing and ripening of Camembert cheese as well as the microbiota present using quantitative reverse transcription polymer chain reaction (RT-qPCR) and 16S rRNA pyrosequencing, respectively...
July 7, 2017: Journal of Microbiology and Biotechnology
José Ramos, Yessica Melero, Laura Ramos-Moreno, Carmen Michán, Lourdes Cabezas
Yeasts, filamentous fungi and bacteria colonize the surface of fermented sausages during the ripening process. The source of this microbiota is their surrounding environment, and it is influenced by their maturing conditions and by starter cultures. Debaryomyces hansenii has been previously isolated from several dry-cured meat products, and it has been associated with the lipolytic and proteolytic changes that occur in these products, which influence their taste and flavour. In this work, the yeast microbiota present in casing from different meat products ("lomo", "chorizo" and "salchichón") from the Valle de los Pedroches region, South-Spain, were isolated for the first time...
July 7, 2017: Journal of Microbiology and Biotechnology
Ji Yeong Park, Seon-Ju Jeong, Jeong A Kim, Jeong Hwan Kim
Some promoters were isolated and characterized from genome of Leuconostoc mesenteroides SY2, an isolate from kimchi, a Korean traditional fermented vegetable. Chromosomal DNA of L. mesenteroides SY2 was digested with Sau3AI and ligated with BamHI cut pBV5030, a promoter screening vector containing a promoter-less cat-86. Among E. coli transformants (TFs) resistant against Cm (chloramphenicol), 17 were able to grow in the presence of 1,000 μg/ml Cm and their inserts were sequenced. Transcription start sites were examined for 3 putative promoters (P04C, P25C, and P33C) by primer extension...
July 7, 2017: Journal of Microbiology and Biotechnology
Ye-Ram Kim, Chul-Su Yang
Despite significant efforts to improve tuberculosis (TB) treatment, it remains a worldwide infectious disease due to the limitations of current TB regimens. Recent work on novel TB treatment strategies has suggested that directly targeting host factors is beneficial for TB treatment. Such strategies, termed host-directed therapeutics (HDTs), focus on host-pathogen interactions. HDTs are more beneficial than existed TB drugs, which are limited the long durations of treatment and the emergence of drug-resistant strains...
July 7, 2017: Journal of Microbiology and Biotechnology
Seung Won Shin, Myunghwan Jung, Ho Geun Won, Kuastros Mekonnen Belaynehe, In Joong Yoon, Han Sang Yoo
The rapid dissemination Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli has significantly contributed to hazard of public health globally. A total of 281 E. coli strains recovered from pigs and chickens between 2009 and 2015 in South Korea were analyzed for ESBL-production. ESBL phenotypes were recognized in 14 E. coli isolates; ten and three ESBL-producing isolates carried only blaCTX-M and blaCMY genes, respectively, and one isolate harbored both genes. The predominant CTX-M- and CMY-types were CTX-M-15 (n=8) and CMY-2 (n=3)...
July 7, 2017: Journal of Microbiology and Biotechnology
Youngjae Cho, Eiseul Kim, Sun-Kyung Han, Seung-Min Yang, Mi-Ju Kim, Hyun-Joong Kim, Chang-Gyeom Kim, Dong-Won Choo, Young-Rok Kim, Hae-Yeong Kim
Vibrio species are generally recognized as pathogens predominant in seafood along coastal areas. The food industry has sought to develop efficient microbial detection methods. Due to the limits of conventional methods, this study aimed to establish a rapid identification method for Vibrio isolated from Korea, based on matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Four different preparation procedures were compared to determine the appropriate means to pre-treat Vibrio species, using 17 isolates and five reference strains...
July 7, 2017: Journal of Microbiology and Biotechnology
Hyunbin Seong, Jieun Heo, Kyun Hee Lee, Yoon Bok Lee, Yun Bae Kim, Nam Soo Han
White rose petal extract (WRE) contains large amounts of phenolic compounds and is considered edible. In this study, red and white wines were prepared by the addition of WRE (0.10% or 0.25%, w/v), followed by fermentation at 25°C for 15 days. The fermentation profiles, colors, sensory test results, and antioxidant activities of wines were compared. As reported herein, the fermentation profiles such as the pH, CO2 production rate, and final ethanol concentration were not affected by the addition of WRE, but a slow consumption rate of sugar was observed in 0...
July 7, 2017: Journal of Microbiology and Biotechnology
Jung-Beom Kim, Ok-Kyung Choi, Sun-Mok Kwon, Seung-Hak Cho, Byung-Jae Park, Na Young Jin, Yong Man Yu, Deog-Hwan Oh
The prevalence and toxin characteristics of Bacillus thuringiensis isolated from 39 organic vegetables were investigated. B. thuringiensis was detected in 30 out of the 39 organic vegetables (76.9%) with a mean value of 2.60 log CFU/g. Twenty-five out of the 30 B. thuringiensis isolates (83.3%) showed insecticidal toxicity against Spodoptera exigua. The hblCDA, nheABC, and entFM were found to be the major toxin genes, but the ces gene was not detected in any of the tested B. thuringiensis isolates. The hemolysin BL enterotoxin was detected in all of 30 B...
July 7, 2017: Journal of Microbiology and Biotechnology
Eun Seob Lim, Joo-Sung Kim
The complex roles of cell surface modification in the biofilm formation of Campylobacter jejuni, a major cause of worldwide foodborne diarrheal disease, are poorly understood. In a screen of mutants from random transposon mutagenesis, an insertional mutation in eptC gene (cj0256) resulted in a significant decrease in C. jejuni NCTC11168 biofilm formation (<20%) on major food contact surfaces, such as polystyrene and borosilicate glass, compared with wild-type cells (p < 0.05). In C. jejuni strain 81-176, the protein encoded by eptC modifies cell surface structures, such as lipid A, the inner core of lipooligosaccharide, and the flagellar rod protein (FlgG), by attaching phosphoethanolamine...
July 7, 2017: Journal of Microbiology and Biotechnology
Sumei Yu, Chunying Teng, Xin Bai, Jinsong Liang, Tao Song, Liying Dong, Yu Jin, Juanjuan Qu
In this study, the siderophore-producing characteristics and conditions of Bacillus sp. PZ-1 were investigated and the enhancement of siderophores on Pb uptake and translocation in Brassica juncea were determined. Results of single factor experiment showed that glucose, pH and Pb(NO3)2 could stimulate PZ-1 growth and siderophore production. The maximum siderophore production of 90.52% siderophore units was obtained by response surface methodology optimization at glucose concentration of 21.84 g/l, pH 6.18 and Pb(NO3)2 concentration of 245...
June 21, 2017: Journal of Microbiology and Biotechnology
Jingyeong Shin, Young Beom Kim, Jong Hun Jeon, Sangki Choi, In Kyu Park, Young Mo Kim
Anaerobic mono- and co-digestion of sewage sludge (SS) and food waste leachate (FWL) were performed by assessing methane production and characterizing microbial communities. Anaerobic digestion (AD) of waste activated sludge (WAS) alone produced the lowest methane production (281 mL-CH4), but an approximately 80% increase in methane production was achieved via co-digestion of WAS and FWL (506 mL-CH4). There were less differences in the diversity of bacterial communities in anaerobic digesters, while archaeal (ARC) and bacterial (BAC) amounts reflected AD's performance...
June 21, 2017: Journal of Microbiology and Biotechnology
Rit Bahadur Gurung, So Youn Gong, Tuoi Thi Le, Dipesh Dhakal, Narae Jung, Hye Jin Jung, Tae Jin Oh, Jae Kyung Sohng
Curcumin is a natural polyphenolic compound, widely acclaimed for its antioxidant, anti-inflammatory, antibacterial and anti-cancerous properties. However, its use has been limited due to low-aqueous solubility and poor bioavailability, rapid clearance and low cellular uptake. In order to assess the effect of glycosylation on pharmacological properties of curcumin, the one-pot multi-enzyme (OPME) chemoenzymatic glycosylation reactions with UDP-α-D-glucose or UDP-α-D-2-deoxyglucose as donor substrate were employed...
June 21, 2017: Journal of Microbiology and Biotechnology
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