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Pakistan Journal of Pharmaceutical Sciences

Amtul Fayyaz, Junaid Ali Khan, Muhammad Mudassar Ashraf, Naheed Akhter, Bilal Aslam, Muhammad Fiaz Khalid, Sidra Altaf, Rana Dawood Naseer, Muhammad Akram, Syed Muhammad Ali Shah, Muhammad Waqas Khadam, Imtiaz Mahmood Tahir
Aim of present study was to investigate the pharmacokinetic behavior of Montelukast in the healthy male volunteers under indigenous conditions. One tablet of montelukast 10 mg was administered in each subject and blood at different time intervals. Concentration of montelukast in plasma samples was analyzed by high performance liquid chromatography method to calculate pharmacokinetic parameters. The plasma concentration of montelukast was in the range of 1.31-1.76μg/mL at 0.5-12 hours with Cmax value of 1.59±0...
November 2017: Pakistan Journal of Pharmaceutical Sciences
Yongjun Hu, Zhaofen Zheng, Hongwei Pan, Jianqiang Peng, Yangping Luo, Bo Cui, Xin Zhong
Programmed cell death plays an important role in cardio protection, and Notch1 was an important factor related to programmed cell death. The role of Notch1 on ischemia myocardium remains unclear.H9C2 myocardial cells were cultured with routine medium, transfected with Notch1 over expression plasmid, Notch1-siRNA-overexpression plasmid and vehicle plasmid for further hypoxic experiment. Condition of hypoxic experiment was 1% oxygen centration and culturing for 12hours, then the cell proliferation activity and apoptosis rate was assessed by MTS kit and flow cytometry, respectively...
November 2017: Pakistan Journal of Pharmaceutical Sciences
Sidrah Shahzad, Saara Ahmad, Syeda Madiha, Saima Khaliq, Laraib Liaquat, Sadia Sadir, Sahar Rafiq, Saiqa Tabassum, Zehra Batool, Saida Haider
Schizophrenia (SZ) is categorized as neuropsychiatric disorder with reduced lifespan and significant impairments in social and vocational functioning. One of the best proposed pharmacological animal models is dizocilpine, as it can mimic the full spectrum of schizophrenic disorder including positive and negative symptoms along with cognitive deficits. Dizocilpine is N-methyl-D-aspartate (NMDA) receptor antagonist known to induce hyper-locomotion and stereotyped behavior in rodents. Present study was designed to develop an animal model of SZ via intraperitoneal administration of dizocilpine in rats (100-150g) at a dose of 0...
November 2017: Pakistan Journal of Pharmaceutical Sciences
Tuba Siddiqui, Iyad Naeem Muhammad, Muhammad Naseem Khan, Shazia Naz, Lubna Bashir, Nosheen Sarosh, Rida Masood, Aatka Ali, Sakina Fatima, Talat Naqvi
This assessment aims to determine the prevalence of methicillin resistance and multidrug resistance (MDR) among the clinical isolates of Staphylococcus aureus and antimicrobial susceptibility profile of methicillin resistant Staphylococcus aureus (MRSA) to the frequently prescribed antibiotics in Karachi. Isolates of MRSA, recovered from various clinical samples were included in this prospective, cross-sectional study from Jan 2015 to June 2017. Agar diffusion method was employed according to the protocols of Clinical Laboratory Standards Institute...
November 2017: Pakistan Journal of Pharmaceutical Sciences
Sabahat Naeem, Shamim Akhtar, Zi-Ning Lei, Kimberly Lu, Shaista Zafar, Ahsaan Ahmed, Mohsin Ali, Mansoor Ahmed, Zhe-Sheng Chen
Cancer is ultimately the result of cells that hysterically grow and do not die. Cells can experience uncontrolled growth if there are mutations to DNA, and therefore, alterations to the genes involved in cell division. Cancer occurs when a cell's gene mutations make the cell unable to correct DNA damage and is unable to destroy itself. There are over 100 different types of cancer each classified by the type of initially affected cell. Isoniazid, a well-known antitubercular agent has been reported to exhibit some cytotoxic activity...
November 2017: Pakistan Journal of Pharmaceutical Sciences
Muhammad Mukhtiar, Syed Umer Jan, Ihsan Ullah, Abid Hussain, Izhar Ullah, Rahman Gul, Essa Ali, Abdul Jabbar, Zulfiqar Hussan Kuthu, Muhammad Wasim, Muhammad Farid Khan
Thiol groups are extensively present across biological systems being found in range of small molecules (e.g. Glutathione, Homo-cysteine) and proteins (e.g. albumin, haemo-globin). Albumin is considered to be a major thiol containing protein present in circulating Plasma. Albumin contains a single thiolate group located at cysteine-34(cys-34) at its active site. Albumin also binds a wide variety of metals and metals complexes at various sites around the protein. Usually heavy metals are preferentially attached with the thiol group of albumin...
November 2017: Pakistan Journal of Pharmaceutical Sciences
Mohsin Ali, Mansoor Ahmed, Saleem Hafiz, Mustafa Kamal, Majid Mumtaz, Muhammad Hanif, Khalid Mohammed Khan
The development and spreading of Multi Drug Resistant TB strains is hampering endeavours for the control and administration of tuberculosis (TB). The expansion episodes of multi-medication safe strains of Mycobacterium tuberculosis against first and second line antituberculosis drugs on one side and the unfavourable effects of these drugs on the other side has led the enthusiasm of researcher towards the synthesis of metal complexes of various medication. This approach is born with the expectation of finding new antituberculous operators without or least reactions as well as being active against the resistant strains of Mycobacterium tuberculosis...
November 2017: Pakistan Journal of Pharmaceutical Sciences
Bushra Uzair, Neelam Firdous, Barkat Ali Khan, Samiullah Khan, Sammer Fatima, Rehana Kausar, Asma Bano
The emergence of multi drug resistant microbial pathogens has become a global health challenge and set a dire requirement of searching new effective antimicrobials. Soil is an ultimate reservoir of biologically active micro flora, which harbors trillions of microbial strains producing compounds of commercial interest. Hence aim of the present study was an attempt to isolate and identify the antibiotic producing microbial strains from the red soil of Himalayan an unexplored region of Pakistan. In this study from 10 different soil samples only one bacterial strain was isolated capable of antimicrobial activity...
November 2017: Pakistan Journal of Pharmaceutical Sciences
Lamiaa A Shaala, Ameen Almohammadi
Investigation of the cytotoxic fraction of the extracts of the Red Sea sponge Suberea sp. resulted in the identification of two new compounds, 1-(hydroxy(1H-pyrrol-2-yl)methyl)guanidine and 4-(2-amino-3-methylbut-3-en-1-yl)phenol (1 and 2) together with the previously reported 2-(3,5-dibromo-4-hydroxyphenyl)acetamide (3), subereaphenol C (4), dibromoverongiaquinol (5) and bromochloroverongiaquinol (6). The compounds were assigned by assignment of their one- and two-dimensional NMR and MS spectral data. The cytotoxic activities of the compounds against two cancer cell lines were evaluated...
November 2017: Pakistan Journal of Pharmaceutical Sciences
Bushra Munir, Bilal Ahmed, Shumaila Kiran, Fatima Jalal, Muhammad Kashif Zahoor, Saba Shehzadi, Sadaf Oranab, Sayed Kashif Kamran, Abdul Ghaffar
Hepatitis C is the most common health problem worldwide and is major cause of death due to proliferation of hepatocellular carcinoma. The medicines available for HCV treatment overcome up-to 95% complications of HCV. However, liver cancer needs some additional care. Normally Sorafenib tosylate 200 mg is recommended for liver cancer. There is no such trial in which this drug could effectively be used in combination of direct acting antivirals for HCV. The study was conducted for HCV patients (n=30) with liver cancer having decompensated stage...
November 2017: Pakistan Journal of Pharmaceutical Sciences
Amber Nawaz, Zehra Batool, Saara Ahmed, Saiqa Tabassum, Saima Khaliq, Bushra Jabeen Mehdi, Irfan Sajid, Shoaib Ahmad, Sadia Saleem, Fizza Naqvi, Faizan Naqvi, Saida Haider
This study was designed to investigate the role of enriched environment in preventing and/or reducing the neurobehavioral deficits produced after nicotine administration in albino Wistar rats. Equal numbers of rat in two groups were either placed in social environment (control group) or social along with physically enriched environment for four weeks before the administration of nicotine. Exposure to different environmental conditions was followed by the intraperitoneal injection of nicotine at the dose of 0...
November 2017: Pakistan Journal of Pharmaceutical Sciences
P N Prasad Maddisetty, V A Doss, Mohanasundaram S, Srinivasarao V, Mohanbabu T
In this study, we have aimed to analyze the phytochemical composition of this plant and the concentration of strychnine and brucine. The identification of bioactive compounds was done by GC-MS with NIST Library. Strychnine and Brucine were quantified using HPLC. Twenty one medicinal bio active compounds were identified from the Strychnos nux-vomica leaf ethanolic extract. Strychnine is showing 28.43% purity and brucine was not detected in GCMS analysis. Quantified the concentration of strychnine (0.6 mg in 500mg of extract) and brucine (1...
November 2017: Pakistan Journal of Pharmaceutical Sciences
Hasan Afarnegan, Ali Shahraki, Jafar Shahraki
Paraquat is extensively used as a strong nitrogen-based herbicide for controlling weeds in agriculture. This herbicide is extremely toxic to humans and induces multiorgan failure due to accumulation in the cells. So far, many instances of fatal poisoning have been reported. Paraquat is metabolized primarily in the liver. Accordingly, the effects of aquatic Levisticum officinale extract on biochemical factors and oxidative status were evaluated in hepatocytes exposed to paraquat in this study. The results showed that paraquat-induced hepatocyte destruction is mediated by reactive oxygen species (ROS) production...
November 2017: Pakistan Journal of Pharmaceutical Sciences
Sana Ghayas, Muhammad Harris Shoaib, Fahad Siddiqui, Rabia Ismail Yousuf, M Ali Masood, Fakhsheena Anjum, Rabia Bushra, Lubna Bashir, Shazia Naz, Iyad Naeem Muhammad
A swift, precise and simple HPLC bioanalytical technique with UV detection was established and validated for quantitative estimation of valsartan in human plasma. The analyte was separated from plasma by protein precipitation with acetonitrile and chromatographically separated on Zorbax SB-C18 (5μm, 4.6mm × 15cm) column. The solvent mixture system consisting of acetonitrile, water and glacial acetic acid (40:59:1 v/v), was pumped using isocratic mode at 1mL/min flow rate. Samples' detection of drug was made spectrophotometrically at a wavelength of 264nm...
November 2017: Pakistan Journal of Pharmaceutical Sciences
Muhammad Adeel Shahzad, Syed Ali Raza Naqvi, Rashid Rasheed, Muhammad Yameen, Fozia Anjum, Muhammad Tauqeer Ahmed, Zaib Hussain, Syed Jawad Hussain Gillani
Benzylpenicillin acts through binding with beta-lactamase enzyme and inhibiting the bacterial cell wall biosynthesis. Therefore, the radiolabeling of benzylpenicillin with lutetium-177 is expected to serve as a theranostic agent for deep-seated bacterial infections. The radiolabeling of benzylpenicillin resulted ~93% radiochemical yield at optimized reaction conditions. Radiochemical purity analysis was tested with the help of Whatman No. 2 paper and instant thin layer chromatography. Biodistribution study with healthy New Zeeland white rabbit revealed moderate accumulation in different organs...
November 2017: Pakistan Journal of Pharmaceutical Sciences
Attaur Rehman, Anwar Ejaz Beg, Rabia Bushra, Maria Ashfaq, Farya Zafar, Huma Ali, Mehwish Rizvi
A simple stability indicating UV-spectrophotometric method has been developed and validated for the determination of cinitapride hydrogen tartrate (CHT) in bulk and solid pharmaceutical dosage form. Drug absorption was measured in different analytical mediums however; maximum absorption was seen in 0.1 N HCl at wavelength (λmax) of 266 nm. The calibration curve was found to be linear over the concentration range from 6 to14μg/mL with the correlation coefficient value (r) of 0.999. The LOD and LOQ were estimated to be 0...
November 2017: Pakistan Journal of Pharmaceutical Sciences
Ikram Ilahi, Ali Muhammad Yousafzai
The present research is aimed to investigate the susceptibility of Cx. quinquefasciatus larvae (2nd and 4th instars), pupae and adults during exposure to various concentrations of C. nardus n-hexane extract. Increase in mortality of larvae and pupae was observed with increase in concentration of the extract solution (R2>0.90). LC50 values of extract for 2nd and 4th instar larvae were 451.8 and 599.6 ppm, respectively. The LC<inf>90</inf> values of extract for 2nd and 4th instar larvae were 2519...
November 2017: Pakistan Journal of Pharmaceutical Sciences
Tehsin Kamal, Muhammad Sarfraz, Mosab Arafat, Momir Mikov, Nisarur Rahman
The aim was to prepare cross linked polymer of 5-fluorouracil loaded microspheres containing guar gum and sodium borate for colon-targeted drug delivery systems. Micro spheres were prepared using emulsification cross linking method. The influence of drug polymer ratio, cross linker agent concentrations and cross linking timing on in vitro drug release and characteristics in terms of drug loading, entrapment efficiency and yielding percentage were investigated. The optimum drug loading, entrapment efficiency and percent yield were obtained from formulations with the lowest content of cross linker agent over 2 h of cross linking timing but with the highest drug to polymer ratio 1:11...
November 2017: Pakistan Journal of Pharmaceutical Sciences
Kun-Hao Hong, Jian-Ke Pan, Hui Xie, Da Guo, Wei-Yi Yang, Hai-Tao Su, Shu-Chai Xu, Jun Liu
This is the first meta-analysis to assess the clinical efficiency, safety and potential advantages regarding the use of ABT drains compared with no drainage which is controversial in total knee arthroplasty (TKA).A comprehensive literature search was carried out in March 2015 using the PubMed, Embase, and Cochrane Library databases. A metaanalysis was carried out on two retrospective comparative studies (RCSs) and five randomized controlled trials (RCTs). The number of patients receiving homologous blood transfusion was the primary outcome of the meta-analysis; the secondary outcome measure was the mean drop in Hb level in comparison to the mean pre-operative HB level, the range of flexion of the knee joint, and infections of the wound after surgery...
November 2017: Pakistan Journal of Pharmaceutical Sciences
Sabira Sultana, Hafiz Muhammad Asif
Hypertension (HTN) or high blood pressure is a medical condition that accounts 9.4 million deaths all over the world every year. It is leading cause of morbidity and mortality worldwide. It increases the risk of cardiovascular diseases, including heart failure, diffuse atherosclerosis, cognitive impairment and dementia. Clinically, synthetic antihypertensive drugs have been used to treat hypertension. However, the efficacy of these drugs is low and also produces side effects which include dry mouth, dizziness, emotional distress, gastrointestinal disturbance, visual disorders etc...
November 2017: Pakistan Journal of Pharmaceutical Sciences
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