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Pakistan Journal of Pharmaceutical Sciences

Sara Qadeer, Shaista Emad, Tahira Perveen, Sarwat Yousuf, Sheeza Sheikh, Yousra Sarfaraz, Sana Sadaf, Saida Haider
Stress has become an integral feature of everyday living. Each individual that lives encounters some manifestation of stress in life. Stress causes certain alterations in the structure and functions of the body and is considered to be a major factor in many health problems. Many synthetic and natural compounds are used for the attenuation of stress induced changes in the body. Medicinal plants are used since ancient times to prevent from neurological disorders. Lavender (Lavandula angustifolia) is very efficacious and possesses the ability to improve several neurological disorders...
July 2018: Pakistan Journal of Pharmaceutical Sciences
Arooj Arshad, Muhammad Anjum Zia, Muhammad Asgher, Faiz Ahmad Joyia, Muhammad Arif
Streptokinase (SK) is a fibrinolytic protein used for the treatment of cardiovascular disorders. In the present study, enhanced production of SK was achieved by determining the optimum fermentation conditions for the maximum growth of Streptococcus agalactiae EBL-31 using response surface methodology (RSM). Four process variables (pH, temperature, incubation time and inoculum size) with five levels were evaluated in 30 experimental runs. Central composite rotatable design (CCRD) was employed to predict the effect of independent variables on SK activity...
July 2018: Pakistan Journal of Pharmaceutical Sciences
Roman Farooq Alvi, Bilal Aslam, Naveed Shahzad, Muhammad Hidayat Rasool, Muhammad Shafique
Antibiotic resistant Klebsiella pneumoniae, is associated with various nosocomial infections that are difficult to treat. This study is designed to find out the patterns of resistance against commonly used antibiotics in K. pneumoniae clinical isolates with special attention to fluoroquinolones. A total number of 200 K. pneumoniae clinical isolates collected from various tertiary care hospitals of Punjab, Pakistan for a span of 1 year were investigated. Isolates were identified biochemically and genetically using VITEK® system and species-specific PCR, respectively...
July 2018: Pakistan Journal of Pharmaceutical Sciences
Shagufta Kamal, Naheed Akhter, Samreen Gul Khan, Shumaila Kiran, Tahir Farooq, Muhammad Akram, Syed Muhammad Ali Shah, Imtiaz Mahmood Tahir, Muhammad Akhlaq, Fahad Said, Jawad Zaheer
Lovastatin is a natural competitive inhibitor of 3-hydroxy-3-methyl glutaryl coenzyme-A (HMG-CoA) reductase and inhibits specifically rate limiting step in cholesterol biosynthesis. Further, lovastatin in comparison with synthetic drugs has no well-reported side effects. Four pure isolated filamentous fungal strains including Aspergillus niger IBL, Aspergillus terreus FFCBP-1053, Aspergillus flavus PML and Aspergillus nidulans FFCBP-014 have been cultured by solid state fermentation (SSF) using rice straw as substrate for the synthesis of lovastatin...
July 2018: Pakistan Journal of Pharmaceutical Sciences
Madiha Moid, Sidra Afzal, Najia Rahim, Tariq Ali, Wajiha Iffat, Lubna Bashir, Shazia Naz
A precise, sensitive and quick High Performance Liquid Chromatographic (HPLC) method for the determination of rosuvastatin calcium in bulk and tablet dosage forms has been validated. The chromatographic scheme involved: Sil-20A auto sampler, LC-20A pump, SPD-20A UV/visible detector with separation attained by C18 column at 40ºC temperature through a mobile phase of acetonitrile and buffer (50:50) at a flow rate of 1.0ml/min. The method is precise (%RSD for intra-day and inter-day extended between 1.06-1.54% and 0...
July 2018: Pakistan Journal of Pharmaceutical Sciences
Zeqing Huang, Nan Wang, Liangyuan Suo, Mingyu Wang
General anesthesia and surgery have been associated with acute cognitive impairment in several elderly individuals. Present study was conducted to determine whether the general anaesthesia exposure and cognitive dysfunction are linked or not. This is a China-based retrospective, population-based and case-control study. Using Chinese database inhabitants of Shenyang, China, incident cases detected with cognitive abnormalities between January 2007 and December 2012 were identified. With respective to every incident case, age- and gender-matched control subject was chosen among the general population pool of Shenyang inhabitants who were not having cognitive anomalies in the year...
July 2018: Pakistan Journal of Pharmaceutical Sciences
Shazia Anwer Bukhari, Muhammad Masood Ahmed, Fozia Anjum, Haseeb Anwar, Syed Ali Raza Naqvi, Tauseef Zahra, Uzma Batool
Prevalence of hepatitis C virus (HCV) has been seen in more than 15% of Pakistani population. For the treatment of this infection, only two medicines, interferon, and ribavirin were approved in 1998. The concerned physicians evaluate side effects of these two antiviral drugs only during the treatment period. The long-term extra hepatic side effects are being neglected. This retrospective study was conducted with reference to induced infertility in HCV treated 40 male patients from the period 2008-2015. Possible effects of interferon therapy on fertility hormones and seminal parameters were assessed...
July 2018: Pakistan Journal of Pharmaceutical Sciences
Javaria Altaf, Muhammad Hidayat Rasool, Saira Akhtar, Maleeha Manzoor, Tahira Younas, Bakhtawer Ansari, Gulraiz Ahmad, Farhat Jabeen, Muhammad Ali, Rizwan Munir
The aim of this study is to explore the presence of antimicrobial bioactive agents in the foot muscle extracts of snails belonging to genus Physa and Ceciloides. Antibacterial activity of foot extracts belonging to species named as P. fontinalis, P. gyrina, P. acuta, C. acicula, C. eulima, C. petitiana, was checked and compared against three bacterial strains i.e. E.coli, P. auroginosa, S. aureus by using disc diffusion method. The results were highly significant with maximum zone of inhibition of 20.10 mm in the P...
July 2018: Pakistan Journal of Pharmaceutical Sciences
Naheed Niaz, Asma Bano, Fehmida Fasim, Rehana Kausar, Barkat Ali Khan, Naheed Zafar, Samiullah Khan, Bushra Uzair
Bacterial resistance is spreading globally due to excessive use of antibiotics, making it one of our times biggest challenges. To address this issue present study was conducted to evaluate the antibacterial activity of copper oxide nanoparticles against methicillin-resistant S. aureus (MRSA). Copper oxide nanoparticles were synthesized by chemical precipitation method and were characterized by UV-Visible, FT-IR spectroscopy, X-ray diffraction (XRD) and Scanning Electron Microscopy. These nanoparticles of 27nm were assessed for antibacterial activity using disc diffusion method...
July 2018: Pakistan Journal of Pharmaceutical Sciences
Shagufta Kamal, Naheed Akhter, Samreen Gul Khan, Shumaila Kiran, Tahir Farooq, Muhammad Akram, Syed Muhammad Ali Shah, Imtiaz Mahmood Tahir, Muhammad Akhlaq, Fahad Said, Jawad Zaheer
Diabetes is a condition where the fasting blood glucose level elevated above the normal range (80-120mg/dL). This increase in blood glucose level may be due to the insulin deficiency i.e. insulin dependent diabetes mellitus (IDDM or type I) or due to insulin resistance i.e. non-insulin dependent diabetes mellitus (NIDDM or type II). Diabetes leads to severe complications in the body even life treating complications e.g. nephropathy, retinopathy, neuropathy increased vascular permeability and delayed wound healing if left untreated...
July 2018: Pakistan Journal of Pharmaceutical Sciences
Sonia Siddiqui, Aiman Kanwal, Khawar Saeed Jamali, Priya Tufail, Aisha Kamal
Interactions of cancer cells with their microenvironment play a significant role in defining the severity of the disease. In search of novel compounds with anti-inflammatory and anticancerous capabilities, the effects of purified compound piperine were investigated in Neuro-2a cell line. The neuronal lineage of Neuro-2a cell line was confirmed by using antibody against β-III tubulin protein. The cells were treated with different concentrations of piperine (μM: 10, 50 and 100) for 48 hrs at 37ºC. A dose of 100 µM was selected that induces a 50% inhibition in the cell growth calculated by MTT and morphometery assays...
July 2018: Pakistan Journal of Pharmaceutical Sciences
Samiullah Burki, - Mehjabeen, Zeba Gul Burki, Zafar Ali Shah, Muhammad Imran, Muhammad Khan
Owing to its pharmacological versatility, the current study focuses the evaluation of Monotheca buxifolia (M. buxifolia) bark crude extract and its fractions for phytochemical and pharmacological analysis. Phytochemical investigation of bark extract was carried out through GC-MS, LC-MS and FT-IR. ICP-OES was used for analyzing essential metals in bark extract. Plant samples were further investigated for their in vitro antioxidant and in vivo neuropharmacological activities in mice. Phytochemical analysis of bark extract revealed the presence of various active constituents such as serotonin, α-tocopherol, 3-deoxyestradiol, ascorbyl palmitate and cirsimaritin...
July 2018: Pakistan Journal of Pharmaceutical Sciences
Haytham Mahmoud Daradka, Omar Flah Khabour, Mohammad Kdaimes Alotaibi
Oxidative stress is a condition that might predispose the individuals to diseases including cancer. The 8-hydroxydeoxyguanosine (8-OHdG) is a marker that reflects oxidative DNA damage in the body. In this study, seven Saudi medicinal plants were investigated for their potential against oxidative DNA damage using the 8-OHdG assay in cultured human lymphocytes. Extracts at 10-100μg/mL from Nigella sativa black seeds, Olea chrysophylla (aerial parts) and Pulicaria crispa (aerial parts) significantly decreased levels of 8-OHdG (P<0...
July 2018: Pakistan Journal of Pharmaceutical Sciences
Naeem Akhtar Virk, Azizur Rehman, Muhammad Athar Abbasi, Sabahat Zahra Siddiqui, Umer Rashid, Javed Iqbal, Muhammad Saleem, Muhammad Ashraf, Wardah Shahid, Syed Adnan Ali Shah
N-(Substituted)-5-(1-(4-methoxyphenylsulfonyl)piperidin-4-yl)-4H-1,2,4-triazol-3-ylthio) acetamide were synthesized by following conventional as well as microwave assisted protocol through five consecutive steps under the impact of various reaction conditions to control the reaction time and the yield of product. Starting from 4-methoxybenzenesulfonyl chloride and ethyl isonipecotate, product 3 was obtained which was converted into product 4 by treating with hydrazine hydrate. In step 3, the product 4 was refluxed with methyl isothiocyanate and KOH to yield compound 5 which was finally treated with variety of N-substituted acetamides to yield an array of different new compounds (8a-k)...
July 2018: Pakistan Journal of Pharmaceutical Sciences
Muhammad Tauqeer Ahmed, Muhammad Yameen, Bushra Munir, Sadia Asim, Muhammad Usman, Syed Ali Raza Naqvi, Jawad Akhtar Hussain Gillani, Rashid Rasheed, Muhammad Adeel Shahzad
Bacterial infection is one of the vital sources of morbidity and mortality. The development of single photon emission computed tomography (SPECT) radiotracer agents using antibiotics, for targeting in-vivo bacteria, helps in antibiotic dose calibration, targeted infection therapy and reduction in mortality rate. The aim of this study was to appraised 99m Tc-labeling sulfadiazine as a radiopharmaceutical for bacillus infections imaging. Radiolabeling of sulfadiazine with technetium-99m was carried out by subsequent addition of 1...
July 2018: Pakistan Journal of Pharmaceutical Sciences
Syed Imran Ali, Nighat Razvi, Syed Mohammad Farid Hasan
Pioglitazone is widely used for the management of type-II diabetes mellitus. The objective of the present study was to develop a simple and cost-effective HPLC method for the quantification of pioglitazone in human plasma. The mobile phase comprises of Acetonitrile, 0.1 M ammonium acetate and glacial acetic acid (25:25:1 v/v/v) at a flow rate of 1.2 mL/min., using Macherey-Nagel Column C18, (dimensions: 5 μm; 250 × 4.6mm) with a guard column. The UV detector was set at 269nm. The method was validated according to FDA guidelines...
July 2018: Pakistan Journal of Pharmaceutical Sciences
Jaweria Anwar, Muhammad Asif Zahoor, Muhammad Kashif Zahoor, Abu Baker Siddique, Zeeshan Nawaz, Muhammad Hidayat Rasool, Muhammad Usman Qamar, Muhammad Waseem, Syed Zajif Hussain, Aysha Yasmin
In current study we investigated the efficacy of organic extracts of Azadirachta indica leaves against Methicillin Resistant Staphylococcus aureus (MRSA) clinical isolates. For this purpose fresh leaves were used to prepare ethanol, methanol and chloroform extract. Secondly, a cross sectional study was conducted to isolate MRSA in clinical samples from patients having surgical/ non-surgical wounds from Allied Hospital and District Head Quarter Hospital, Faisalabad. The S. aureus isolates were initially identified by biochemical characterization, followed by identification of MRSA using cefoxitin disc diffusion test that was finally confirmed by genomic amplification of mecA gene, responsible for resistance...
July 2018: Pakistan Journal of Pharmaceutical Sciences
Tahira Younis, Azhar Rasul, Faiza Jabeen, Ghulam Hussain, Javaria Altaf, Laila Jafri, Rehana Rani, Muhammad Rashid Khan, Iqra Sarfraz, Muhammad Ali
Roots, bark, stem/twigs, and leaves of Fraxinus xanthoxyloides are being used regionally for the cure of malaria, jaundice, internal injuries, pneumonia, pain, rheumatism and also in fracture of bones. Our objective was to assess the methanolic leaves extract of F. xanthoxyloides for its antioxidant capability against oxidative stress induced by carbon tetrachloride (CCl4 ) in the kidney of Sprague-Dawley rats. Duration of this experiment was 30 days and doses were given on alternative days. Urine of rats was assessed for kidney function and renal tissues for antioxidant enzymes activity, biochemical markers, comet assay and histopathology...
July 2018: Pakistan Journal of Pharmaceutical Sciences
Farya Zafar, Sohail Khan, Huma Ali, Shabana N Shah, Rabia Bushra, Ghazala Raza Naqvi, Kashif Maroof, Maqsood Ahmed Khan, Zebun Nisa
In this research work biowaiver studies of newly developed and optimized Meloxicam 7.5mg and 15mg water dispersible formulations were carried out at different dissolution media i.e. 0.1N HCl, phosphate buffer pH 4.5, pH 6.8, and pH 7.5 at 50 rpm. For this purpose reference (MA9 and MB9 ) and tests (MA2 , MA4 , MA6 , MA7 and MA8 (15 mg) and MB2, MB4, MB6, MB7and MB8 (7.5 mg) formulations were compared. In vitro patterns were analyzed by using model-independent and model-dependent methods. Results indicated that all formulation at pH 0...
July 2018: Pakistan Journal of Pharmaceutical Sciences
Muhammad Jalil Khan, Muhammad Mukhtiar, Muhammad Murtaza Qureshi, Syed Umer Jan, - IzharUllah, Abid Hussain, Muhammad Farid Khan, Rahman Gul, Nisar Ahmad Shahwani, Imran Rabbani
Thallium has been shown to significantly influence various tissues of living organisms; Exposure to Thallium can disturb mitochondrial function, degenerate neurons, and interfere with the function of critical metabolic enzymes and co-enzymes. Glutathione (GSH) an essential biomarker is considered a key factor in harnessing the thallium toxicity. In the present study the interaction of Thallium (Thallium Chloride) and glutathione was investigated spectro-photo-metrically in aqueous media. The renowned Elman's experimental protocol was followed at a wavelength of 412nm for Glutathione quantification in each sample...
July 2018: Pakistan Journal of Pharmaceutical Sciences
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