Dihua Tian, Xin Qi, Maral Seididamyeh, Huayue Zhang, Anh Phan, Zexi Zhang, Xuhui Geng, Yasmina Sultanbawa, Run Zhang
The development of reliable probe technology for the detection of bisulfite (HSO3 - ) in situ in food and biological samples is contributing significantly to food quality and safety assurance as well as community health. In this work, a responsive probe, EHDI, is developed for ratiometric fluorescence detection of HSO3 - in aqueous solution, meat samples, and living cells. The probe is designed based on the HSO3 - triggered 1,4-addition of electron deficit C = C bond of EHDI. As a result of this specific 1,4-addition, the π-conjugation system was destructed, resulting in blue shifts of the emission from 687 to 440 nm and absorption from 577 to 355 nm...
March 31, 2024: Methods: a Companion to Methods in Enzymology