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Journal de Mycologie Médicale

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https://www.readbyqxmd.com/read/29779647/in-vitro-susceptibility-and-multilocus-sequence-typing-of-fusarium-isolates-causing-keratitis
#1
P Dallé da Rosa, A Nunes, R Borges, B Batista, A Meneghello Fuentefria, L Z Goldani
Fungal keratitis is recognized as a significant cause of ocular morbidity and blindness especially in developing countries. In this study, we aimed to present the molecular identification and susceptibility of Fusarium isolates causing fungal keratitis in a university hospital in southern Brazil. The samples were identified using the second largest subunit of the RNA polymerase gene (RPB2) and the translation elongation factor 1-alpha (TEF1), while the antifungal susceptibility was tested by the broth microdilution method according to the Clinical and Laboratory Standards Institute (CLSI) methodology...
May 17, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29778633/comparative-analysis-of-proteinase-phospholipase-hydrophobicity-and-biofilm-forming-ability-in-candida-species-isolated-from-clinical-specimens
#2
S Dabiri, M Shams-Ghahfarokhi, M Razzaghi-Abyaneh
Candida species are the commensal organisms of human and animal mucosa that cause a wide range of debilitating diseases in immunocompromised patients and other susceptible individuals. The present study aimed to investigate the ability of clinical isolates of various Candida species to produce proteinase and phospholipase, hydrophobicity and biofilm forming ability that assumed to have a vital role in Candida pathogenicity. Eighty-four Candida strains belonged to Candida albicans (44.1%), C. glabrata (5.9%), C...
May 16, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29773436/non-sinonasal-isolated-facio-orbital-mucormycosis-a-case-report
#3
S K Swain, M C Sahu, A Banerjee
Mucormycosis is a rare clinical entity, often affect immunocompromised patients. It is an emergency situation and has poor prognosis. Prompt diagnosis with tissue biopsy, local control of the disease by aggressive surgical debridement and appropriate systemic antifungal treatment improve the prognosis and survival of the patients. Treatment of mucormycosis needs antifungal agents such as Amphotericin B and wide surgical debridement. Early diagnosis and treatment is often needed for survival of the patients...
May 14, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29773435/epidemiology-of-antifungal-susceptibility-review-of-literature
#4
REVIEW
I Hadrich, A Ayadi
Fungal infections are a major cause of morbidity and mortality despite the latest developments of diagnostic tools and therapeutic options. Early initiation of the appropriate antifungal therapy has been demonstrated to have a direct impact on the patient's outcome. Antifungal susceptibility testing methods are available to detect antifungal resistance and to determine the best treatment for a specific fungus. American and European standards have been developed, as well as equivalent commercial systems, which are more appropriate for clinical laboratories...
May 14, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29773434/tinea-blepharo-ciliaris-in-a-13-year-old-girl-caused-by-trichophyton-benhamiae
#5
Fang-Ying Wang, Pei-Lun Sun
Tinea blepharo-ciliaris is a rare form of dermatophyte infection which involves eyelids and associated eyelashes. We report a 13-year-old girl with type I diabetes mellitus who had right eyelid swelling and eyelash loss for two weeks. The lesions were presented as erythematous patches with scales and tiny pustules on the right upper and lower eyelids with broken eyelashes. Two additional annular erythematous patches with scaly active borders were found on her right forearm and right thigh. Microscopic examination of broken eyelashes demonstrated many chains of arthroconidia and hyaline hyphae in an endothrix invasion pattern...
May 14, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29773433/-cerebral-and-pulmonary-nocardiosis-to-nocardia-abscessus-in-an-immunocompetent-algerian-patient
#6
D Arrache, H Zait, V Rodriguez-Nava, E Bergeron, T Durand, M Yahiaoui, F Grenouillet, A Amrane, F Chaouche, A Baiod, K Madani, B Hamrioui
Nocardial brain abscess is often occurring in immunocompromised patients. It is uncommon in immunocompetent individuals. Here, the authors describe a case of cerebral and pulmonary nocardiosis mimicking a metastatic tumor in an apparently health 40-year-old Algerian male. The patient presented multiple brain abscess revealed by inaugural epileptic seizure. He was afebrile and presented with left hemiparesis. Staging imaging showed a nodular lung lesion in the apical segment of the right lower lobe. The patient underwent double craniotomy for resection of the lesion...
May 14, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29753721/efficacy-of-modified-leeming-notman-media-in-a-resazurin-microtiter-assay-in-the-evaluation-of-in-vitro-activity-of-fluconazole-against-malassezia-furfur-atcc-14521
#7
F E Far, M M J Al-Obaidi, M N M Desa
BACKGROUND: Malassezia furfur is lipodependent yeast like fungus that causes superficial mycoses such as pityriasis versicolor and dandruff. Nevertheless, there are no standard reference methods to perform susceptibility test of Malassezia species yet. AIMS: Therefore, in this study, we evaluated the optimized culture medium for growth of this lipophilic yeast using modified leeming-Notman agar and colorimetric resazurin microtiter assay to assess antimycotic activity of fluconazole against M...
May 9, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29730129/anticandidal-synergistic-activity-of-ocimum-sanctum-and-fluconazole-of-azole-resistance-strains-of-clinical-isolates
#8
K U Zaidi, F Shah, R Parmar, V Thawani
Candida albicans is the most prevalent fungal pathogen in humans. It is the causative agent and most associated with serious fungal infection, accounting for more than 90% of cases. It is a most common cause of deep mycoses and vulvovaginal candidiasis. In the present study we found that methanolic extract of O. sanctum in combination of fluconazole shows higher zone of inhibition and lesser MIC values as compared to methanolic extract of leaves of O. sanctum or fluconazole when used alone. Synergistic antimicrobial activity was found when methanolic extract of leaves of O...
May 2, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29724543/fusarium-solani-infection-as-an-initial-manifestation-of-aml-transformation-in-myelodysplastic-syndrome-a-case-report
#9
D P Borges, A W A Santos, S M M Magalhaes, J J Sidrim, M F G Rocha, R A Cordeiro, R S N Brilhante, S P Bandeira, J T Valença Junior, R F Pinheiro
Severely immunocompromised patients are at increased risk for uncommon infectious diseases with atypical presentations. Fusarium sp., has been reported in patients with hematological malignancies and prompt diagnosis is necessary due to high mortality. We report a myelodysplastic syndrome (MDS) patient who presented Fusarium solani infection associated with granulocytic sarcoma as an initial presentation of acute myeloid leukemia (AML) transformation. We performed histological examination, immunohistochemistry analysis, culture of the biopsy tissue and DNA sequencing to make a conclusive diagnosis of F...
April 30, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29709267/orbital-cellulitis-secondary-to-a-fungal-sinusitis-caused-by-scopulariopsis-the-first-case-in-tunisia
#10
S Kammoun, M Rekik, H Trabelsi, S Neji, J Feki, A Ayadi
We report a case of invasive fungal sinusitis caused by Scopulariopsis in 57 year-old man who had recurrence of orbital cellulitis. CT-scan and magnetic resonance imaging found an orbital cellulitis associated to a left frontal sinusitis with bone erosion and calcification. Patient was treated by surgical debridement and voriconazole. Culture of excised tissue was positive for Scopulariopsis.
April 27, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29709266/saprochaete-capitata-geotrichum-capitatum-an-emerging-fungal-infection-in-kidney-transplant-recipients
#11
N Rizk, Z Hajar, W Medawar
We are reporting the case of an 82-year-old Yemeni patient, renal transplant recipient who was admitted to our institution and who subsequently developed disseminated infection with Saprochaete capitata. This pathogenic fungus is rarely reported in patients with solid organ trans-plants. Saprochaete capitata is an emerging fungal pathogen, ubiquitously spread in the environment. This is the second case to our knowledge of infection with Saprochaete capitata in a renal transplant patient. Our patient was treated for multiple nosocomial infections with prolonged antibiotic courses...
April 27, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29685520/in-vitro-susceptibility-of-candida-spp-to-fluconazole-itraconazole-and-voriconazole-and-the-correlation-between-triazoles-susceptibility-results-from-a-five-year-study
#12
J Lei, J Xu, T Wang
Candida spp. is a common cause of invasive fungal disease. The aim of this study was to examine the susceptibility of Candida spp. to fluconazole, itraconazole and voriconazole and explore the correlation between triazoles susceptibility. The antifungal susceptibility in the present study was measured by ATB Fungus 3 method, and the potential relationship was examined by obtaining the correlation of measured minimal inhibitory concentrations (MICs) of Candida spp. isolates. A total of 2099 clinical isolates of Candida spp...
April 20, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29673771/posaconazole-vs-voriconazole-in-the-prevention-of-invasive-fungal-diseases-in-patients-with-haematological-malignancies-a-retrospective-study
#13
L Tang, X-F Yang, M Qiao, L Zhang, X-W Tang, H-Y Qiu, D-P Wu, A-N Sun
BACKGROUND: Posaconazole is superior to fluconazole or itraconazole in preventing invasive fungal diseases (IFDs) in patients with haematological malignancies; however, there have been reports of the comparing posaconazole and voriconazole. METHODS: This single-centre, retrospective study in China enrolled AML, ALL and MDS patients, among others. Prophylaxis with posaconazole or voriconazole was administered for patients recovering from neutropenia or who had achieved complete remission...
April 16, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29673770/the-in-vitro-efficacy-of-sunsmile-%C3%A2-fruit-vegetable-rinse-against-pathogenic-strains-of-prototheca-algae-that-cause-mastitis-in-cows
#14
B Grzesiak, H Krukowski, A Głowacka
The research concerns algae of the genus Prototheca. They are found in the natural environment and they can cause a disease in animals and humans called protothecosis. The aim of the study was to evaluate the in vitro activity of the fruit and vegetable rinse agent SunSmile® Fruit & Vegetable Rinse (Sunrider International) against P. zopfii isolates. The materials consisted of ten P. zopfii strains isolated from the milk of cows with mastitis. The following antifungal chemotherapeutic agents were also used in the study for comparison: nystatin, ketoconazole, amphothericin B, miconazole, clotrimazole, econazole, fluconazole, and flucytosine...
April 16, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29673769/successful-treatment-of-a-recurrent-aspergillus-niger-otomycosis-with-local-application-of-voriconazole
#15
M Chappe, S Vrignaud, L de Gentile, G Legrand, F Lagarce, Y Le Govic
Fungal otitis (otomycosis) is a common infection encountered by otolaryngologists. Nevertheless, its management can be challenging because of its high recurrence rate and of the limited therapeutic options. A 45-year-old woman suffered from recurrent otomycosis. The ineffectiveness of successive antibiotic cures and repeated topical treatments with nystatin and then with econazole cream led to perform microbiological analyses. Culture of ear swab grew Aspergillus niger. The use of a 1% voriconazole sterile solution previously validated for treatment of eye infections was considered after ensuring the absence of known ototoxic effects of the antifungal and of the excipients...
April 16, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29661606/potential-effect-of-2-isopropyl-5-methylphenol-thymol-alone-and-in-combination-with-fluconazole-against-clinical-isolates-of-candida-albicans-c-glabrata-and-c-krusei
#16
A Sharifzadeh, A R Khosravi, H Shokri, H Shirzadi
Limitations of antifungals used in the treatment of candidiasis, as the development of resistant strains, are known by the scientific community. In this context, the aim of this study was to investigate the activity of 2-isopropyl-5-methylphenol (thymol) in combination with fluconazole (FLZ) against clinical Candida strains. The antifungal activity of thymol along with FLZ was evaluated by the Clinical Laboratory Standards Institute (CLSI) M27-A2 broth microdilution method. In addition, synergism was observed for clinical strains of Candida spp...
April 14, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29661607/catheter-related-bloodstream-infection-due-to-rhodotorula-mucilaginosa-with-normal-serum-1%C3%A2-3-%C3%AE-d-glucan-level
#17
T Kitazawa, S Ishigaki, K Seo, Y Yoshino, Y Ota
Rhodotorula species are environmental basidiomycete yeasts that have emerged as a cause of fungemia in immunocompromised hosts. The insertion of a central venous catheter was identified as a major risk factor for Rhodotorula fungemia. Few cases reports have reported (1→3)-β-D-glucan testing at the onset of Rhodotorula mucilaginosa fungemia. We report a case of catheter-related bloodstream infection due to R. mucilaginosa. Serum β-D-glucan level was normal at the onset of the bloodstream infection. It took 5 days to culture the isolate...
April 13, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29650464/clioquinol-an-alternative-antimicrobial-agent-against-common-pathogenic-microbe
#18
Z You, X Ran, Y Dai, Y Ran
Skin and soft tissue infections (SSTIs) are very common in dermatology and the use of antimicrobial formulations are important in treating these diseases. With the increasing of drug-resistant strains, researchers need to find ways to enhance the effectiveness and/or reduce the drug resistance. Clioquinol was one of antiseptics that can inactivate microbes. It was lack of data of antimicrobial activity; meanwhile it was infrequently used in infection. In order to research the antimicrobial spectrum and activity of topical 3% clioquinol cream among common pathogenic microorganisms compared with other common topical pharmaceuticals, we used modified agar diffusion assay to judge drug susceptibility and compared with broth microdilution assay...
April 10, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29628352/-identification-of-yeasts-from-commercial-atti%C3%A3-k%C3%A3-in-abidjan-c%C3%A3-te-d-ivoire-preliminary-study
#19
C G Kouadio-Yapo, G S P Dou, N A D Aka, K D Zika, K D Adoubryn, M Dosso
OBJECTIVE: This study aims to identify yeasts from commercial attiéké. PATIENTS AND METHODS: This descriptive cross-sectional survey involving 103 samples of attiéké sold on the market from August to September 2017. It was conducted from the mycology laboratory of the Institut Pasteur of Côte d'Ivoire. The samples were seeded on YGC medium. The colonies of positive culture were counted and seeded again on chromogen medium. Identification of yeasts species was carried out by MALDI-TOF mass spectrometry (Vitek MS bioMérieux)...
April 6, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29605542/non-culture-based-assays-for-the-detection-of-fungal-pathogens
#20
REVIEW
S Otašević, S Momčilović, N M Stojanović, M Skvarč, K Rajković, V Arsić-Arsenijević
Traditional, culture based methods for the diagnosis of fungal infections are still considered as gold standard, but they are time consuming and low sensitive. Therefore, in order to overcome the limitations, many researchers have focused on the development of new immunological and molecular based rapid assays that could enable early diagnosis of infection and accurate identification of fungal pathogens causing superficial and invasive infection. In this brief review, we highlighted the advantages and disadvantages of conventional diagnostic methods and possibility of non-culture based assays in diagnosis of superficial fungal infections and presented the overview on currently available immunochromatographic assays as well as availability of biomarkers detection by immunodiagnostic procedures in prompt and accurate diagnosis of invasive fungal infections...
March 29, 2018: Journal de Mycologie Médicale
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