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Revista Iberoamericana de Micología

Janire De-La-Torre, Guillermo Quindós, Cristina Marcos-Arias, Xabier Marichalar-Mendia, María Luisa Gainza, Elena Eraso, Amelia Acha-Sagredo, José Manuel Aguirre-Urizar
BACKGROUND: Candida can be implicated in the pathology of chronic periodontitis. AIMS: To analyze the oral Candida carriage in patients suffering from chronic periodontitis (CP) and its correlation with the severity of this condition. METHODS: Microbiological samples were taken from 155 patients using the oral rinse (OR) technique and by using paper points in the periodontal pockets (GPP). These patients were divided into 3 groups: 89 patients without CP (control), 47 with moderate CP, and 19 with severe CP...
August 3, 2018: Revista Iberoamericana de Micología
Ivana Maldonado, Silvana Cataldi, Claudia Garbasz, Silvia Relloso, Pablo Striebeck, Liliana Guelfand, Laura López Moral
BACKGROUND: Invasive fungal infections are increasing, and Candida yeasts are the main cause. Species other than Candida albicans are becoming more frequent, and some of them may have variable patterns of susceptibility to antifungal agents, making it important to identify them correctly. Conventional identification methods used by most laboratories may present with drawbacks. Mass spectrometry (MALDI-TOF MS) has emerged as an alternative method. AIMS: The aim of this study was to evaluate the concordance of the identification, at species level, by conventional methods (API) and MALDI-TOF MS...
August 3, 2018: Revista Iberoamericana de Micología
Rosa Belén Mohedano Del Pozo, Margarita Rubio Alonso, Mª Soledad Cuétara García
BACKGROUND: The role of culture-independent techniques (galactomannan, (1-3)-β-d-glucan) in the early diagnosis of invasive fungal diseases (IFD) is well assessed in hematological patients, but there are no clear conclusions in patients with chronic obstructive pulmonary disease (COPD). AIMS: To study the usefulness of nonculture-based techniques in the diagnosis of IFD in COPD-patients at risk for IFD. METHODS: A prospective observational study based on monitoring COPD patients at risk for IFD during 2007-2010 was carried out...
August 2, 2018: Revista Iberoamericana de Micología
Susan Maria, Gaurav Barnwal, Anil Kumar, Karthika Mohan, Vivek Vinod, Aswathi Varghese, Raja Biswas
BACKGROUND: Candida parapsilosis is recognized as a species complex: Candida parapsilosis sensu stricto, Candida orthopsilosis and Candida metapsilosis are three distinct but closely related species. AIMS: To determine the species and antifungal susceptibility of members of the C. parapsilosis complex, isolated from clinical samples. METHODS: Isolates identified as C. parapsilosis complex by VITEK® 2 system were included. Antifungal susceptibility test was done using the VITEK® 2 semi-automated system...
July 8, 2018: Revista Iberoamericana de Micología
Berta Pino-Calm, Diego García Martínez de Artola, Helena Gil-Campesino, Julia Alcoba-Flórez
No abstract text is available yet for this article.
April 3, 2018: Revista Iberoamericana de Micología
María Virginia Ozcariz-Fermoselle, Raúl Fraile-Fabero, Tomás Girbés-Juan, Oscar Arce-Cervantes, Juan Andrés Oria de Rueda-Salgueiro, Anabela Marisa Azul
BACKGROUND: The wastes of pecan nut (Carya illinoinensis (Wangenh.) K. Koch) production are increasing worldwide and have high concentrations of tannins and phenols. AIMS: To study the biodegradation of lignocellulosic wastes of pecan used as solid substrate for the cultivation of the white-rot fungus Ganoderma lucidum (Curtis) P. Karst. METHODS: Six formulations of pecan wastes were used as solid substrate: pecan shells (PS100), pecan pericarp (PP100), pecan wood-chips (PB100), and the combinations PS50+PP50, PB50+PS50 and PB50+PP50...
April 2018: Revista Iberoamericana de Micología
José Luis Barrios Andrés, Leyre Mónica López-Soria, Ana Alastruey Izquierdo, Jaime Echevarría Ecenarro, Raquel Feijoó Lera, Jesus Garrido Fierro, Francisco Javier Cabrerizo Nuñez, Andrés Canut Blasco
BACKGROUND: Although fortunately very rare in countries with a temperate climate, certain factors, such as clinical or pharmacological immunosuppression, may cause Fusarium-related fungal infections to become an emerging problem. Moreover, Fusarium is one of the most important etiological agents in exogenous endophthalmitis, which is often favored by the disruption of the epithelial barriers. AIMS: The aim of this series of clinical cases is to identify characteristic clinical findings that may allow an early diagnosis and more efficient management of this ophthalmologic emergency...
April 2018: Revista Iberoamericana de Micología
Laura Theill, Catiana Dudiuk, Soraya Morales-Lopez, Indira Berrio, José Yesid Rodríguez, Adriana Marin, Soledad Gamarra, Guillermo Garcia-Effron
BACKGROUND: Candida auris and Candida haemulonii are emerging and multiresistant pathogens. C. auris has produced hospital outbreaks and is misidentified by phenotypic-based methods. The only reliable identification methods are DNA sequencing and MALDI-TOF. AIMS: To develop a classical-PCR method capable of rapidly and accurately identify C. auris and C. haemulonii. METHODS: A multiplex PCR was carried out in one tube that included an internal control and oligonucleotides that specifically hybridize to the ITS2 region of C...
April 2018: Revista Iberoamericana de Micología
Saeid Amanloo, Masoomeh Shams-Ghahfarokhi, Mohammad Ghahri, Mehdi Razzaghi-Abyaneh
BACKGROUND: Candida glabrata is a yeast that can cause hazardous fungal infections with high mortality and drug resistance. AIMS: The aim of this study was to determine the profile of drug susceptibility in clinical isolates of C. glabrata and review the resistance mechanisms to caspofungin. METHODS: A total of 50 C. glabrata clinical isolates from Iran were tested for in vitro susceptibilities to amphotericin B, caspofungin, fluconazole and voriconazole...
April 2018: Revista Iberoamericana de Micología
Iker Falces-Romero, Inmaculada Quiles-Melero, Julio García-Rodríguez
No abstract text is available yet for this article.
April 2018: Revista Iberoamericana de Micología
Mariana Mazza, Nicolás Refojo, Graciela Davel, Nelson Lima, Nicolina Dias, Cosme Marcelo Furtado Passos da Silva, Cristina Elena Canteros
BACKGROUND: No reliable data are available in the province of Buenos Aires regarding the frequency of dermatophytoses and other fungal diseases. The distribution of the clinical forms and the species involved are also unknown. AIMS: To present the data collected by the laboratories participating in the Mycology Network of the province of Buenos Aires (MNPBA) from a retrospective epidemiological survey on dermatophytoses. METHODS: A descriptive and exploratory analysis was performed on the cases of dermatophytoses gathered between 2002 and 2007 by the Mycology Network of the province of Buenos Aires...
April 2018: Revista Iberoamericana de Micología
Felipe F B Tuon, Khaiany Lino Florencio, Clóvis Arns da Cunha, Jaime Luis Lopes Rocha
BACKGROUND: Posaconazole is used for the prophylaxis of invasive fungal disease (IFD). Previous studies have shown it to be cost-effective compared to fluconazole/itraconazole. However, posaconazole has never been economically evaluated in developing countries. AIMS: The aim of the present study was to perform a cost-effectiveness analysis of posaconazole compared to fluconazole in public (SUS) and private hospitals (PHS) in Brazil. METHODS: A cost-effectiveness simulation was conducted on the basis of a pivotal study on the use of posaconazole in acute myeloid leukemia (AML) patients, adjusting the costs to Brazilian data...
April 2018: Revista Iberoamericana de Micología
Angel Bernal-Treviño, Ana María González-Amaro, Verónica Méndez González, Amaury Pozos-Guillen
BACKGROUND: Microbiological identification in endodontic infections has focused mainly on bacteria without giving much attention to yeasts, which, due to their virulence factors, can affect the outcomes of root canal treatment. AIMS: To determine the frequency of Candida in anaerobic conditions in root canals with primary and persistent endodontic infection, as well as to evaluate a microbiological sampling method using aspiration compared to the traditional absorption method with paper points...
April 2018: Revista Iberoamericana de Micología
Ricardo Andreotti Siqueira, Andre Mario Doi, Paulo Petrus de Petrus Crossara, Paula Celia Mariko Koga, Alexandre Gimenes Marques, Fabiane Gomes Nunes, Jacyr Pasternak, Marines Dalla Valle Martino
BACKGROUND: An increased incidence of fungal infections caused by Candida species, especially Candida glabrata and Candida krusei, which are less susceptible to azoles, has been observed. Standardized susceptibility testing is essential for clinical management and for monitoring the epidemiology of resistance. AIMS: We evaluated the performance of two different susceptibility testing commercial methods, Vitek 2® and Sensititre YeastOne® , and compared them with the standard broth microdilution method (CLSI)...
April 2018: Revista Iberoamericana de Micología
Komathy Munusamy, Jamuna Vadivelu, Sun Tee Tay
BACKGROUND: Biofilm is known to contribute to the antifungal resistance of Candida yeasts. Aureobasidin A (AbA), a cyclic depsipeptide targeting fungal sphingolipid biosynthesis, has been shown to be effective against several Candida species. AIMS: The aim of this study was to investigate Candida biofilm growth morphology, its biomass, metabolic activity, and to determine the effects of AbA on the biofilm growth. METHODS: The biofilm forming ability of several clinical isolates of different Candida species from our culture collection was determined using established methods (crystal violet and XTT assays)...
April 2018: Revista Iberoamericana de Micología
Bryan Ortiz, Erika Pérez-Alemán, Carmen Galo, Gustavo Fontecha
BACKGROUND: Candiduria is a common infection among hospitalised patients. Although the clinical relevance of yeasts in urine is not clearly defined, fungal urinary tract infections have increased significantly in the last decades, becoming a growing public health problem. Candida albicans is the most commonly reported species in urinary infections, although other species of the genus are becoming particularly important, because some of them are linked with resistance to antifungal drugs...
April 2018: Revista Iberoamericana de Micología
Leticia Aguilar Doroteo, Paola Berenice Zárate Segura, Ramón Villanueva Arce, Jorge Yáñez Fernández, María Eugenia Garín Aguilar, Paula Cecilia Guadarrama Mendoza, Gustavo Valencia Del Toro
BACKGROUND: Molecular characterisation of wild type Pleurotus species is important for germplasm conservation and its further use for genetic improvement. No molecular studies have been performed with monokaryons used for producing hybrid strains, either with the reconstituted strains obtained by pairing those monokaryons. The molecular characterisation of parental dikaryons, hybrid, and reconstituted strains as well as monokaryotic strains, is therefore of utmost importance. AIMS: To carry out the molecular identification of Pleurotus djamor strains, i...
January 2018: Revista Iberoamericana de Micología
Rogelio de J Treviño-Rangel, Cynthia D Peña-López, Pedro A Hernández-Rodríguez, Dinael Beltrán-Santiago, Gloria M González
BACKGROUND: Candidemia is one of the most common nosocomial infections globally and it is associated with considerable excess mortality and costs. Abreast, biofilm-forming strains are associated with even higher mortality rates and poor prognosis for the patient. AIMS: To evaluate a possible association between the biofilm-forming capability of Candida bloodstream isolates and the clinical evolution in patients with candidemia. METHODS: An observational, retrospective study was conducted at a tertiary care university hospital during 9 years (2006-2015)...
January 2018: Revista Iberoamericana de Micología
Rogelio de J Treviño-Rangel, Byron A Bodden-Mendoza, Alexandra M Montoya, Hiram Villanueva-Lozano, Mariana Elizondo-Zertuche, Efrén Robledo-Leal, Gloria M González
BACKGROUND: Candida tropicalis is an increasingly important human pathogen which usually affects neutropenic oncology patients with common hematogenous seeding to peripheral organs and high mortality rates. Candida pathogenicity is facilitated by several virulence attributes, including secretion of hydrolytic enzymes; however, little is known regarding the C. tropicalis ability to secrete them and their role in the disease. AIMS: To confirm by molecular means the identification of 187 clinical isolates (127 from blood, 52 from urine, and 8 from diverse clinical origins) phenotypically identified as C...
January 2018: Revista Iberoamericana de Micología
Alexandra M Montoya, Mariana Elizondo-Zertuche, Rogelio de J Treviño-Rangel, Miguel Becerril-García, Gloria M González
BACKGROUND: Trichosporon asahii is a yeast-like fungus that has recently gained importance as a cause of opportunistic systemic infections. The pathogenicity and virulence factors of T. asahii remain largely unknown. Because of the association between invasive infections and the use of catheters and related devices, the ability of the microorganism to adhere and form biofilms may play an important role in the pathogenicity during a trichosporonosis. AIMS: The aim of this study is to identify an association between biofilm formation by T...
January 2018: Revista Iberoamericana de Micología
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