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Revista Iberoamericana de Micología

Komathy Munusamy, Jamuna Vadivelu, Sun Tee Tay
BACKGROUND: Biofilm is known to contribute to the antifungal resistance of Candida yeasts. Aureobasidin A (AbA), a cyclic depsipeptide targeting fungal sphingolipid biosynthesis, has been shown to be effective against several Candida species. AIMS: The aim of this study was to investigate Candida biofilm growth morphology, its biomass, metabolic activity, and to determine the effects of AbA on the biofilm growth. METHODS: The biofilm forming ability of several clinical isolates of different Candida species from our culture collection was determined using established methods (crystal violet and XTT assays)...
March 12, 2018: Revista Iberoamericana de Micología
Bryan Ortiz, Erika Pérez-Alemán, Carmen Galo, Gustavo Fontecha
BACKGROUND: Candiduria is a common infection among hospitalised patients. Although the clinical relevance of yeasts in urine is not clearly defined, fungal urinary tract infections have increased significantly in the last decades, becoming a growing public health problem. Candida albicans is the most commonly reported species in urinary infections, although other species of the genus are becoming particularly important, because some of them are linked with resistance to antifungal drugs...
January 30, 2018: Revista Iberoamericana de Micología
Leticia Aguilar Doroteo, Paola Berenice Zárate Segura, Ramón Villanueva Arce, Jorge Yáñez Fernández, María Eugenia Garín Aguilar, Paula Cecilia Guadarrama Mendoza, Gustavo Valencia Del Toro
BACKGROUND: Molecular characterisation of wild type Pleurotus species is important for germplasm conservation and its further use for genetic improvement. No molecular studies have been performed with monokaryons used for producing hybrid strains, either with the reconstituted strains obtained by pairing those monokaryons. The molecular characterisation of parental dikaryons, hybrid, and reconstituted strains as well as monokaryotic strains, is therefore of utmost importance. AIMS: To carry out the molecular identification of Pleurotus djamor strains, i...
January 17, 2018: Revista Iberoamericana de Micología
Rogelio de J Treviño-Rangel, Byron A Bodden-Mendoza, Alexandra M Montoya, Hiram Villanueva-Lozano, Mariana Elizondo-Zertuche, Efrén Robledo-Leal, Gloria M González
BACKGROUND: Candida tropicalis is an increasingly important human pathogen which usually affects neutropenic oncology patients with common hematogenous seeding to peripheral organs and high mortality rates. Candida pathogenicity is facilitated by several virulence attributes, including secretion of hydrolytic enzymes; however, little is known regarding the C. tropicalis ability to secrete them and their role in the disease. AIMS: To confirm by molecular means the identification of 187 clinical isolates (127 from blood, 52 from urine, and 8 from diverse clinical origins) phenotypically identified as C...
December 26, 2017: Revista Iberoamericana de Micología
Rogelio de J Treviño-Rangel, Cynthia D Peña-López, Pedro A Hernández-Rodríguez, Dinael Beltrán-Santiago, Gloria M González
BACKGROUND: Candidemia is one of the most common nosocomial infections globally and it is associated with considerable excess mortality and costs. Abreast, biofilm-forming strains are associated with even higher mortality rates and poor prognosis for the patient. AIMS: To evaluate a possible association between the biofilm-forming capability of Candida bloodstream isolates and the clinical evolution in patients with candidemia. METHODS: An observational, retrospective study was conducted at a tertiary care university hospital during 9 years (2006-2015)...
January 2018: Revista Iberoamericana de Micología
Alexandra M Montoya, Mariana Elizondo-Zertuche, Rogelio de J Treviño-Rangel, Miguel Becerril-García, Gloria M González
BACKGROUND: Trichosporon asahii is a yeast-like fungus that has recently gained importance as a cause of opportunistic systemic infections. The pathogenicity and virulence factors of T. asahii remain largely unknown. Because of the association between invasive infections and the use of catheters and related devices, the ability of the microorganism to adhere and form biofilms may play an important role in the pathogenicity during a trichosporonosis. AIMS: The aim of this study is to identify an association between biofilm formation by T...
January 2018: Revista Iberoamericana de Micología
Myrna Sabanero López, Lérida L Flores Villavicencio, Karla Soto Arredondo, Gloria Barbosa Sabanero, Julio César Villagómez-Castro, Gustavo Cruz Jiménez, Gerardo Sandoval Bernal, Haydee Torres Guerrero
BACKGROUND: Sporotrichosis is a fungal infection caused by the Sporothrix schenckii complex. The adhesion of the fungus to the host tissue has been considered the key step in the colonization and invasion, but little is known about the early events in the host-parasite interaction. AIMS: To evaluate the proteolytic activity of S. schenckii on epithelial cells. METHODS: The proteolytic system (at pH 5 and 7) was evaluated using azocoll and zymograms...
January 2018: Revista Iberoamericana de Micología
Roseli Santos de Freitas, Camila Mika Kamikawa, Adriana Pardini Vicentini
BACKGROUND: Current methods for the production of Histoplasma capsulatum antigens are problematic in terms of standardization, specificity, stability, repeatability and reproducibility. AIMS: In this study, we sought to optimize the methodology for producing H. capsulatum antigens, and to evaluate its applicability. METHODS: Antigenic preparations obtained from 12 H. capsulatum isolates were evaluated by double immunodiffusion and immunoblotting assays against homologous and heterologous sera...
January 2018: Revista Iberoamericana de Micología
Fernando Messina, Roxana Depardo, Ricardo Negroni, Mercedes Romero, Laura Walker, Alicia Arechavala, Emmanuel Marín, Cristina Canteros, Gabriela Santiso
The case of a 60 year old woman with hemoptysis and a thin-walled cavitary lesion at the upper lobe of the right lung is presented. The woman presented at the Mycology Unit of the Muñiz Hospital in Buenos Aires City 3 months after the beginning of her clinical manifestations. A hyaline micelial fungus with chlamido-arthroconidias was isolated from the bronchoalveolar lavage. Immunodiffusion and counter-immnunoelectrophoresis with coccidioidin and histoplasmin rendered positive results against both antigents, and skin tests with coccidioidin and histoplasmin were also positive with strong reactions...
January 2018: Revista Iberoamericana de Micología
Mercedes Romero, Fernando Messina, Emmanuel Marín, Alicia Arechavala, Ricardo Negroni, Roxana Depardo, Laura Walker, Andrés Benchetrit, Gabriela Santiso
A 48 year-old immunocompetent woman, who had a nodular lesion in the neck and a dense infiltrate at the lower lobe of the left lung, presented at the Mycology Unit of Muñiz Hospital of Buenos Aires City. The pulmonary infiltrate disappeared spontaneously 3 months later. The histopathological study of the nodular lesion showed capsulated yeasts (mucicarmin and alcian blue positive stains) compatible with Cryptococcus. The mycological study of a new sample, obtained by a nodular puncture, allowed the isolation of yeasts, identified as Cryptococcus gattii (VGII)...
January 2018: Revista Iberoamericana de Micología
Mariana Greco, Alejandro Pardo, Graciela Pose, Andrea Patriarca
BACKGROUND: Xerophilic fungi represent a serious problem due to their ability to grow at low water activities causing the spoiling of low and intermediate moisture foods, stored goods and animal feeds, with the consequent economic losses. AIMS: The combined effect of water activity and temperature of four Eurotium species isolated from animal feeds was investigated. METHODS: Eurotium amstelodami, Eurotium chevalieri, Eurotium repens and Eurotium rubrum were grown at 5, 15, 25, 37 and 45°C on malt extract agar adjusted with glycerol in the range 0...
November 11, 2017: Revista Iberoamericana de Micología
Alicia Arechavala, Ricardo Negroni, Fernando Messina, Mercedes Romero, Emmanuel Marín, Roxana Depardo, Laura Walker, Gabriela Santiso
BACKGROUND: Cryptococcosis is still a life-threatening mycosis that continues to be of serious concern in Latin American countries, especially among HIV+positive population. However, there is not any reliable information about the prevalence of this disease in this region. AIMS: The aim of this study is to report data of 2041 patients with cryptococcosis that were attended at the Infectious Diseases Hospital F. J. Muñiz over a 30 year-period. METHODS: Information about demographic and clinical data, survival time and the applied treatment, was taken from the Mycology Unit database...
November 9, 2017: Revista Iberoamericana de Micología
Guillermo Quindós, Javier Pemán
No abstract text is available yet for this article.
October 2017: Revista Iberoamericana de Micología
María Del Rocío Reyes-Montes, Esperanza Duarte-Escalante, Erick Martínez-Herrera, Gustavo Acosta-Altamirano, María Guadalupe Frías-De León
This study presents a systematic review of the literature on the etiology of superficial and invasive candidiasis in Mexico reported from 2005 to 2015. The data have shown that Candida albicans is the most prevalent species with an increasing tendency of the non-C. albicans Candida species, as reported in other countries. The use of phenotypical methods in the identification of the yeasts limits the identification at the species level, particularly in species that are part of complexes, this is important because the identification only at the genus level leads to inadequate treatment due to the different susceptibility to the antifungals among species...
October 2017: Revista Iberoamericana de Micología
Rafael Henrique Fernandes, Everaldo Antônio Lopes, Darlan Ferreira Borges, Amanda Ferreira Bontempo, José Cola Zanuncio, José Eduardo Serrão
BACKGROUND: Exposure of the nematophagous fungus Pochonia chlamydosporia to solar radiation and elevated temperatures before being incorporated into the soil can reduce its survival and efficiency as biocontrol agent. AIMS: A field experiment was carried out to assess the effect of the exposure period on the viability of P. chlamydosporia applied on the soil surface. METHODS: A commercial bionematicide based on P. chlamydosporia was sprayed on soil, and soil samples were collected before and at 0, 30, 60, 90, 120, and 150min after fungal application...
September 25, 2017: Revista Iberoamericana de Micología
Luis Eduardo Ruiz-González, Juan Antonio Vázquez-Zea, Fernando Vega-Villasante, Laura Guzmán-Dávalos, Saúl Rogelio Guerrero-Galván
BACKGROUND: The consumption of wild mushrooms has increased in recent years. However, not all mushrooms are edible and some of them may cause poisoning. Therefore, their toxicity needs to be studied. Artemia franciscana is a crustacean used in toxicity tests including toxins of fungi. AIMS: To determine the percentage of inhibition and mortality produced by extracts of several basidiomycetes on the hatching of A. franciscana cysts. METHODS: Aqueous extracts were prepared from 15 species of mushrooms collected from Jalisco state, Mexico...
August 19, 2017: Revista Iberoamericana de Micología
Ramón Fernández, Roberto Arenas, Esperanza Duarte-Escalante, María Guadalupe Frías-De León, María Elisa Vega Memige, Gustavo Acosta Altamirano, María Del Rocío Reyes-Montes
BACKGROUND: Coccidioidomycosis is one of the most important endemic mycoses in Northern Mexico. However, diagnosing this disease can be challenging, particularly in patients who do not reside in endemic areas. CASE REPORT: The case of a Mexican HIV+ patient who developed fever, general malaise, a severe cough, and dyspnea during a stay in Acapulco, Guerrero, Mexico, is presented. Since various diseases are endemic to the state of Guerrero, the doctors originally suspected that the patient had contracted influenza A (H1N1), Q fever, or tuberculosis...
August 4, 2017: Revista Iberoamericana de Micología
Dulce Iliana Navarro Vergara, Gloria Barragán Pola, Alexandro Bonifaz, Carlos Núñez Pérez-Redondo, Omar Choreño García, Raúl Cicero Sabido
BACKGROUND: Pulmonary mucormycosis is a rare opportunistic infection with high mortality that is caused by species of Mucorales. The most common species involved are Rhizopus, Mucor, Lichtheimia, and Rhizomucor. CASE REPORT: A 56 year-old woman presented with a clinical history of diabetes mellitus type 2 and chronic renal disease. She underwent a cadaveric kidney transplantation two years before her admission, for which immunomodulating therapy with thymoglobulin, tacrolimus, mofetil-microphenolate and prednisone was established...
July 27, 2017: Revista Iberoamericana de Micología
Elisabeth Gómez Moyano, Leandro Martínez Pilar, Silvestre Martinez Garcia, Vicente Crespo Erchiga
No abstract text is available yet for this article.
July 27, 2017: Revista Iberoamericana de Micología
Felipe Francisco Tuon, Khaiany Lino Florencio, Jaime L Rocha, Francisco Beraldi Magalhaes
No abstract text is available yet for this article.
July 22, 2017: Revista Iberoamericana de Micología
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