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Croatian Medical Journal

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https://www.readbyqxmd.com/read/28613042/allele-frequencies-of-15-str-loci-in-bosnian-and-herzegovinian-population
#1
Amela Pilav, Naris Pojskić, Anesa Ahatović, Mirela Džehverović, Jasmina Čakar, Damir Marjanović
AIM: To determine newest the most accurate allele frequencies for 15 short tandem repeat (STR) loci in the Bosnian and Herzegovinian population, calculate statistical parameters, and compare them with the relevant data for seven neighboring populations. METHODS: Genomic DNA was obtained from buccal swabs of 1000 unrelated individuals from all regions of Bosnia and Herzegovina. Genotyping was performed using PowerPlex® 16 System to obtain allele frequencies for 15 polymorphic STR loci including D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX, and FGA...
June 14, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28613041/genotyping-horse-epithelial-cells-from-fecal-matter-by-isolation-of-polymerase-chain-reaction-products
#2
Pero Dimsoski
AIM: To show that application of the polymerase chain reaction (PCR) method modified for amplification of a low-copy number DNA samples, ie, the isolation of PCR products (IPCRp), would represent improvement in obtaining genotypes from a fecal DNA compared with previously used genotyping methods. METHODS: The DNA from the horse fecal matter was extracted by modified Qiagen DNA Stool Mini Kit protocol. Following the extraction, the DNA genotypes from fecal samples were obtained by the most powerful PCR amplification method, the IPCRp...
June 14, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28613040/chromosomal-microarray-in-clinical-diagnosis-a-study-of-337-patients-with-congenital-anomalies-and-developmental-delays-or-intellectual-disability
#3
Ivona Sansović, Ana-Maria Ivankov, Adriana Bobinec, Mijana Kero, Ingeborg Barišić
AIM: To determine the diagnostic yield and criteria that could help to classify and interpret the copy number variations (CNVs) detected by chromosomal microarray (CMA) technique in patients with congenital and developmental abnormalities including dysmorphia, developmental delay (DD) or intellectual disability (ID), autism spectrum disorders (ASD) and congenital anomalies (CA). METHOD: CMA analysis was performed in 337 patients with DD/ID with or without dysmorphism, ASD, and/or CA...
June 14, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28613039/sex-estimation-standards-for-medieval-and-contemporary-croats
#4
Željana Bašić, Ivana Kružić, Ivan Jerković, Deny Anđelinović, Šimun Anđelinović
AIM: To develop discriminant functions for sex estimation on medieval Croatian population and test their application on contemporary Croatian population. METHODS: From a total of 519 skeletons, we chose 84 adult excellently preserved skeletons free of antemortem or postmortem changes and took all standard measurements. Sex was estimated/determined using standard anthropological procedures and ancient DNA (amelogenin analysis) where pelvis was insufficiently preserved or where sex morphological indicators were not consistent...
June 14, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28613038/investigation-of-the-str-loci-noise-distributions-of-powerseq%C3%A2-auto-system
#5
Xiangpei Zeng, Jonathan L King, Bruce Budowle
AIM: To characterize the noise and stutter distribution of 23 short tandem repeats (STRs) included in the PowerSeqTM Auto System. METHODS: Raw FASTQ files were analyzed using STRait Razor v2s to display alleles and coverage. The sequence noise was divided into several categories: noise at allele position, noise at -1 repeat position, and artifact. The average relative percentages of locus coverage for each noise, stutter, and allele were calculated from the samples used for this locus noise analysis...
June 14, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28613037/results-of-a-collaborative-study-on-dna-identification-of-aged-bone-samples
#6
Daniel Vanek, Bruce Budowle, Jitka Dubska-Votrubova, Angie Ambers, Jan Frolik, Martin Pospisek, Ahmed Anwar Al Afeefi, Khalid Ismaeil Al Hosani, Marie Allen, Khudooma Saeed Al Naimi, Dina Al Salafi, Wafa Ali Rashid Al Tayyari, Wendy Arguetaa, Michel Bottinelli, Magdalena M Bus, Jan Cemper-Kiesslich, Olivier Cepil, Greet De Cock, Stijn Desmyter, Hamid El Amri, Hicham El Ossmani, Ruth Galdies, Sebastian Grün, Francois Guidet, Anna Hoefges, Cristian Bogdan Iancu, Petra Lotz, Alessandro Maresca, Marion Nagy, Jindrich Novotny, Hajar Rachid, Jessica Rothe, Marguerethe Stenersen, Mishel Stephenson, Alain Stevanovitch, Juliane Strien, Denilce R Sumita, Joanna Vella, Judith Zander
AIM: A collaborative exercise with several institutes was organized by the Forensic DNA Service (FDNAS) and the Institute of the Legal Medicine, 2nd Faculty of Medicine, Charles University in Prague, Czech Republic, with the aim to test performance of different laboratories carrying out DNA analysis of relatively old bone samples. METHODS: Eighteen laboratories participating in the collaborative exercise were asked to perform DNA typing of two samples of bone powder...
June 14, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28613036/a-tribute-to-dragan-primorac-on-the-twentieth-anniversary-of-the-international-society-of-applied-biological-sciences-and-its-tenth-biennial-conference
#7
EDITORIAL
Stanimir Vuk-Pavlović, Moses Schanfield
No abstract text is available yet for this article.
June 14, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28422458/publication-ethics-standards-should-be-the-same-for-all-peer-reviewed-journals
#8
LETTER
Armen Yuri Gasparyan
No abstract text is available yet for this article.
April 19, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28422457/echoes-of-the-sarajevo-declaration-on-integrity-and-visibility-of-scholarly-publications
#9
LETTER
Simeon Grazio, Branimir Anić, Frane Grubišić
No abstract text is available yet for this article.
April 19, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28409501/successful-treatment-of-fulminant-lyme-myocarditis-with-mechanical-circulatory-support-in-a-young-male-adult-a-case-report
#10
Željko Župan, Dino Mijatović, Igor Medved, Snježana Kraljić, Jurica Juranić, Berislav Barbalić, Marin Oštrić
We describe the case of fulminant myocarditis due to Lyme disease and use of mechanical circulatory support (MCS) for the treatment of the Lyme carditis associated with refractory cardiogenic shock. Fulminant Lyme myocarditis in young adult male patient led to a sudden onset of acute, severe biventricular heart failure with progressive cardiogenic shock, and multiorgan failure immediately after admission.
April 14, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28409500/tissue-protective-activity-of-selenomethionine-and-d-panthetine-in-b16-melanoma-bearing-mice-under-doxorubicin-treatment-is-not-connected-with-their-ros-scavenging-potential
#11
Rostyslav R Panchuk, Nadia R Skorokhyd, Yuliya S Kozak, Liliya V Lehka, Andrey G Moiseenok, Rostyslav S Stoika
AIM: To evaluate molecular mechanisms of tissue-protective effects of antioxidants selenomethionine (SeMet) and D-pantethine (D-Pt) applied in combination with doxorubicin (Dx) in B16 melanoma-bearing-mice. METHODS: Impact of the chemotherapy scheme on a survival of tumor-bearing animals, general nephro- and hepatotoxicity, blood cell profile in vivo, and ROS content in B16 melanoma cells in vitro was compared with the action of Dx applied alone. Nephrotoxicity of the drugs was evaluated by measuring creatinine indicator assay, hepatotoxicity was studied by measuring the activity of ALT/AST enzymes, and myelotoxicity was assessed by light microscopic analysis of blood smears...
April 14, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28409499/use-of-placental-vascularization-indices-and-uterine-artery-peak-systolic-velocity-in-early-detection-of-pregnancies-complicated-by-gestational-diabetes-chronic-or-gestational-hypertension-and-preeclampsia-at-risk
#12
Ábel T Altorjay, Andrea Surányi, Tibor Nyári, Gábor Németh
AIM: We aimed to investigate correlations between uterine artery peak systolic velocity (AUtPSV), and placental vascularization in groups of normal blood pressure (NBP) and hypertensive disorders of pregnancy (chronic hypertension (CHT), gestational hypertension (GHT) and preeclampsia (PE)) alone or in combination with gestational diabetes mellitus (GDM), and hypothesized that AUtPSV rises when GDM complicates pregnancy hypertension. METHODS: Placental 3-dimensional power Doppler indices, such as vascularization index (VI), flow index (FI), and vascularization-flow index (VFI), and uterine artery peak systolic velocity (AUtPSV) were measured in CHT (N=43), CHT+GDM (N=15), GHT (N=57), GHT+GDM (N=23) and PE (N=17) pregnancies, and compared to NBP (N=109)...
April 14, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28409498/differential-pro-apoptotic-effects-of-synthetic-4-thiazolidinone-derivative-les-3288-doxorubicin-and-temozolomide-in-human-glioma-u251-cells
#13
Lesya I Коbylinska, Olga Yu Klyuchivska, Iryna I Grytsyna, Natalia Finiuk, Rostyslav R Panchuk, Marina O Starykovych, Lilya Lehka, Roman B Lesyk, Borys S Zіmenkovsky, Rostyslav S Stoika
AIM: To compare various pro-apoptotic effects of synthetic 4-thiazolidinone derivative (Les-3288), doxorubicin (Dox) and temozolomide (TMZ) in the treatment of human glioma U251 cells to improve treatment outcomes of glioblastoma and avoid anticancer drug resistance. METHODS: The cytotoxic effects of drugs used in human glioma U251 cells were measured by cell viability and proliferation assay (MTT), Trypan blue exclusion test, and Western-blot analysis of the apoptosis-related proteins...
April 14, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28409497/identification-of-myoelectric-signals-of-pregnant-rat-uterus-new-method-to-detect-myometrial-contraction
#14
Kálmán F Szűcs, György Grosz, Miklós Süle, Anikó Nagy, Zita Tiszai, Reza Samavati, Róbert Gáspár
AIM: To develop an electromyography method for pregnant rat uterus in vivo and to separate myometrial signals from the gastrointestinal tract signals. METHODS: Pregnant Sprague-Dawley rats (n=8) were anaesthetized and their stomach, small intestine, and large intestine were removed from the abdomen. A pair of thread electrodes was inserted into the uterus, while a pair of disk electrodes was placed subcutaneously above the myometrium. Additionally, a strain gauge sensor was fixed on the surface of the myometrium and cecum for the parallel detection of mechanical contractions in rats (n=18) with intact gastrointestinal tract...
April 14, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28409496/4-thiazolidinone-derivative-les-3833-effectively-inhibits-viability-of-human-melanoma-cells-through-activating-apoptotic-mechanisms
#15
Nataliya Finiuk, Nataliya Boiko, Olga Klyuchivska, Lesya Коbylinska, Iryna Kril, Borys Zimenkovsky, Roman Lesyk, Rostyslav Stoika
AIM: To evaluate cytotoxic action of 4-thiazolidinone derivative Les-3833 and study the mechanisms of its pro-apoptotic action toward human melanoma cells and human tumor cell lines of other tissue origin. METHODS: The effect of Les-3833 or doxorubicin on the viability of 9 cell lines was studied using MTT assay, while human melanoma cells of WM793 line were additionally examined using light and fluorescent microscopies for evaluating cytomorphological changes. The Western-blot and flow cytometric analyses were carried out to study signaling pathways of melanoma cell cycling and death...
April 14, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28409495/antiplatelet-and-anti-proliferative-action-of-disintegrin-from-echis-multisquamatis-snake-venom
#16
Volodymyr Chernyshenko, Natalia Petruk, Darya Korolova, Ludmila Kasatkina, Olha Gornytska, Tetyana Platonova, Tamara Chernyshenko, Andriy Rebriev, Olena Dzhus, Liudmyla Garmanchuk, Eduard Lugovskoy
AIM: To purify the platelet aggregation inhibitor from Echis multisquamatis snake venom (PAIEM) and characterize its effect on platelet aggregation and HeLa cell proliferation. METHODS: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) were used for PAIEM identification. Platelet aggregation in the presence of PAIEM was studied on aggregometer Solar-AP2110. The changes of shape and granularity of platelets in the presence of PAIEM were studied on flow cytometer COULTER EPICS XL, and degranulation of platelets was estimated using spectrofluorimetry...
April 14, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28409494/association-between-metabolically-healthy-central-obesity-in-women-and-levels-of-soluble-receptor-for-advanced-glycation-end-products-soluble-vascular-adhesion-protein-1-and-the-activity-of-semicarbazide-sensitive-amine-oxidase
#17
Ivana Koborová, Radana Gurecká, Melinda Csongová, Katarína Volkovová, Éva Szökő, Tamás Tábi, Katarína Šebeková
AIM: To determine the levels of circulating soluble receptor for advanced glycation end products (sRAGE), as a biomarker of risk of metabolic syndrome and cardiovascular disease development in centrally obese (CO) women considered metabolically healthy (COH) in comparison with those metabolically unhealthy (COU). METHODS: 47 lean healthy, 17 COH (presenting waist-to-height ratio ≥0.5 but not elevated blood pressure, atherogenic lipid profile, and insulin resistance), and 50 COU (CO presenting ≥2 risk factors) women aged 40-45 years were included...
April 14, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28409493/obesity-in-pregnancy-a-novel-concept-on-the-roles-of-adipokines-in-uterine-contractility
#18
REVIEW
Judit Hajagos-Tóth, Eszter Ducza, Reza Samavati, Sandor G Vari, Robert Gaspar
Obesity is a global health problem even among pregnant women. Obesity alters quality of labor, such as preterm labor, prolonged labor, and higher oxytocin requirements in pregnant women. The most important factors to play a role in the altered gestational period and serve as drug targets to treat the consequences are female sexual hormones, calcium channels, adrenergic system, oxytocin, and prostaglandins. However, we have limited information about the impact of obesity on the pregnant uterine contractility and gestation time...
April 14, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28409492/resisting-small-cake-phenomenon-sharing-resources-and-knowledge-makes-you-rich
#19
EDITORIAL
Srećko Gajović
No abstract text is available yet for this article.
April 14, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28409491/obesity-rubensian-beauty-turned-into-major-health-problem
#20
EDITORIAL
Sandor G Vari
No abstract text is available yet for this article.
April 14, 2017: Croatian Medical Journal
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